| adhesion-induced tyrosine phosphorylation of the p130 src substrate. | adhesion of cells to the extracellular matrix leads to an increase in the tyrosine phosphorylation of a specific set of proteins, three of which have now been identified as the focal adhesion proteins pp125fak, paxillin and tensin. in addition, we have previously noted the adhesion-induced tyrosine phosphorylation of a fourth protein, with an apparent molecular mass of 130. as in the case of fak, paxillin and tensin, a 130 kda protein is also found to be highly tyrosine phosphorylated in rous sa ... | 1995 | 7542255 |
| isolation and characterization of a highly attenuated respiratory syncytial virus (rsv) vaccine candidate by mutagenesis of the incompletely attenuated rsv a2 ts-1 ng-1 mutant virus. | ts-1, a temperature sensitive (ts) mutant of rsv, was previously derived from rsv a2 virus by mutagenesis with 5-fluorouracil (5-fu). ts-1 was attenuated for adult volunteers and seropositive children but retained a low level of virulence in seronegative infant vaccinees as indicated by the occurrence of upper respiratory tract disease. ts-1 ng-1, a more defective derivative of ts-1, was produced by mutagenesis of ts-1 with nitrosoguanidine. however, ts-1 ng-1 still retained a low level of virul ... | 1995 | 7543716 |
| cell-mediated immune responses of lambs to challenge with bovine respiratory syncytial virus. | the lamb is a good model to study the pathogenesis and immune responses to infections with respiratory syncytial virus (rsv) as lambs experimentally infected with bovine or human rsv may develop overt clinical disease. in the present study the development of cellular cytotoxic responses was studied in splenic, pulmonary and peripheral blood mononuclear cells obtained from lambs after primary and secondary infection with bovine rsv. infection with bovine rsv was followed by the appearance of cyto ... | 1995 | 7544251 |
| evaluation of abbott testpack rsv and an in-house rsv elisa for detection of respiratory syncytial virus in respiratory tract aspirates. | the abbott testpack rsv was compared with an in-house rsv enzyme-linked immunosorbent assay (elisa) for detection of respiratory syncytial virus (rsv) antigen. nasopharyngeal specimens were obtained from 121 inpatients. rsv antigen was detected in 46 specimens by the abbott testpack, 42 of these being confirmed by the in-house rsv elisa. of the 75 specimens tested negative in the abbott testpack rsv, one was found positive by the in-house rsv elisa. the sensitivity and specificity of the abbott ... | 1995 | 7546643 |
| [experimental and clinical study of shuanghuanglian aerosol in treating acute respiratory tract infection]. | two hundrde and two cases of acute respiratory tract infection (ari) were treated with shuanghuanglian (shl) aerosol. in these cases, the majority of cases were virus infection and 64% of them was caused by the respiratory syncytial virus (rsv). the virostatic and bacteriostatic test were done in vitro by the cell culture method and it was shown that shl could inhibit the rsv, para-influenza i-iv and 23 kinds of pathogenic bacteria such as staphylococcus aureus etc. the bacteriostatic effect was ... | 1995 | 7549385 |
| respiratory syncytial virus replication in human lung epithelial cells: inhibition by tumor necrosis factor alpha and interferon beta. | respiratory syncytial virus (rsv) is the major pathogen causing severe lung disease in children. rsv initially replicates efficiently in the respiratory tract but becomes undetectable by 7 to 21 d after infection in normal children, suggesting that intrinsic cellular mechanisms, as yet undefined, may restrict virus replication. to provide an in vitro model to examine mechanisms that restrict rsv replication, three human lung epithelial cell lines were exposed to rsv in vitro and virus replicatio ... | 1995 | 7551395 |
| immunity and immunopathology to respiratory syncytial virus. the mouse model. | infection with respiratory syncytial virus (rsv) is a major unsolved challenge for vaccine development. rsv is worldwide in distribution and infects almost all children during the first 2 yr of life. the mouse model of rsv lung disease has been very successful in reproducing many aspects of the human disease. in particular, the role of antiviral t cells in both eliminating virus and causing enhanced disease has been shown dramatically. this immunopathologic paradox is now more clearly understood ... | 1995 | 7551415 |
| pathogenesis of respiratory syncytial virus vaccine-augmented pathology. | respiratory syncytial virus (rsv) is an important respiratory pathogen for which vaccine development has been thwarted by the legacy of vaccine-enhanced illness. a formalin-inactivated, alum-precipitated, whole virus vaccine did not protect children from infection and was associated with severe illness. clues from clinical studies of rsv and vaccine-induced atypical measles illness suggest that an aberrant cd4+ lymphocyte response occurred in vaccinees. there is a growing body of evidence from m ... | 1995 | 7551416 |
| the induction of respiratory syncytial virus-specific cytotoxic t-cell responses following immunization with a synthetic peptide containing a fusion peptide linked to a cytotoxic t lymphocyte epitope. | previously published work has shown that a cytotoxic t-cell epitope (ctl) representing residues 82-90 of the m2 protein of respiratory syncytial virus (rsv) is the target for a protective response against the virus. in this report, we demonstrate that a synthetic peptide representing residues 81-95, when covalently linked to a fusion peptide derived from the conserved n-terminal 19 residues of the f1 protein of measles virus efficiently primes rsv-specific ctls in vivo following immunization wit ... | 1995 | 7558120 |
| cytokine (il-8, il-6, tnf-alpha) and soluble tnf receptor-i release from human peripheral blood mononuclear cells after respiratory syncytial virus infection. | during the initial phase of respiratory syncytial virus (rsv) infection, when a low virus-cell ratio is most probable, signs of inflammation are detectable in the infected respiratory tissue. therefore we analysed the release of the proinflammatory cytokines interleukin-6 (il-6), il-8, tumour necrosis factor-alpha (tnf-alpha), and the soluble form of the tnf receptor-i (stnfr-i), from peripheral blood mononuclear cells (pbmc) after exposure to low infectious rsv doses (multiplicity of infection, ... | 1995 | 7558123 |
| respiratory syncytial virus--is it preventable? | respiratory syncytial virus infection spreads readily among hospital wards, nurseries and intensive care units. careful handwashing is the cornerstone in the prevention of nosocomial transmission. use of rapid diagnostic tests permits the cohorting of patients and staff which has been shown to limit the spread in hospital wards. in addition, the use of gowns and gloves and limiting visitors have been recommended. in the future passive immunization with respiratory syncytial virus (rsv) immune gl ... | 1995 | 7560988 |
| identification of the respiratory syncytial virus-induced immunosuppressive factor produced by human peripheral blood mononuclear cells in vitro as interferon-alpha. | respiratory syncytial virus (rsv) can inhibit the proliferative response of human peripheral blood mononuclear cells (pbmc) in vitro. this inhibition is mediated by an extracellular rsv-induced factor. in the present study, the factor was clearly identified as interferon (ifn)-alpha. the rsv-induced ifn-alpha bound strongly to pbmc and inhibited the anti-rsv proliferative response only when added within the first few days of stimulation. there was, however, no concomitant decrease in the product ... | 1995 | 7561210 |
| transcriptional stimulation of the retina-specific qr1 gene upon growth arrest involves a maf-related protein. | the avian neural retina (nr) is derived from proliferating neuroectodermal precursors which differentiate after terminal mitosis and become organized in cell strata. proliferation of postmitotic nr cells can be induced by infection with rous sarcoma virus (rsv) and requires the expression of a functional v-src protein. qr1 is a retina-specific gene expressed exclusively at the stage of growth arrest and differentiation during retinal development. in nr cells infected with tspa101, an rsv mutant ... | 1995 | 7565708 |
| diaphragmatic flutter in three babies with bronchopulmonary dysplasia and respiratory syncytial virus bronchiolitis. | abnormalities of respiratory control, especially apnea, have been reported previously in infants with respiratory syncytial virus (rsv) infections. this is the first report of yet another abnormality of respiratory control, diaphragmatic flutter (df), in infants with rsv infection. the presentation of these infants did not differ from the usual clinical presentation of rsv infection. while being monitored with respiratory inductive plethysmography for occurrences of apnea known to be common in r ... | 1995 | 7567207 |
| a cotton rat model of effectors of immunity to respiratory syncytial virus other than serum antibody. | a model for studying effectors of immunity to respiratory syncytial virus (rsv) was developed. paris of inbred cotton rats (sigmodon hispidus) were joined surgically using the technique of parabiosis. one week later, one animal of each pair was primed intranasally with a small volume of rsv suspension. fourteen days after priming, both animals of each pair were bled for determination of serum neutralizing antibody titers, and challenged intranasally with a standard dose of rsv suspension. single ... | 1995 | 7567215 |
| microbial inciters of acute asthma in urban nigerian children. | in tropical africa the role of microbial agents of acute respiratory infections in acute exacerbations of bronchial asthma remains largely unexplored. however, empirical antibacterial therapy is frequently initiated in moderate to severe cases of acute asthma with symptoms of acute respiratory infection. a study was set up to determine how often acute respiratory infection is associated with acute asthma, to identify the associated pathogens, and to proffer appropriate therapeutic suggestions. | 1995 | 7570407 |
| different kinetics of antibody responses between iga and igg classes in nasopharyngeal secretion in infants and children during primary respiratory syncytial virus infection. | the secretory antibody responses in 34 infants and children (20 days-17 months old) with lower respiratory tract disease following primary respiratory syncytial virus (rsv) infection were determined using a sensitive tissue culture enzyme linked immunosorbent assay. none of the patients in the acute phase showed iga antibody responses. in contrast significant igg antibody responses which were thought to be maternally derived were observed in infants younger than 2 months of age. in the convalesc ... | 1995 | 7572146 |
| prior infection by respiratory syncytial virus or parainfluenza viruses augments virus-specific igg responses induced by the measles/mumps/rubella vaccine. | we found previously that immunizing cyclophosphamide-treated mice with one paramyxoviridae virus mixed with dimethyl dioctadecyl ammonium bromide induces t cells which apparently also recognize other paramyxoviridae viruses. this finding and the fact that respiratory syncytial virus (rsv) and parainfluenza viruses (pivs) infect children early in life led us to ask if prior rsv or piv infections influence the antibody response to measles and mumps vaccine viruses. detection of virus-specific igg ... | 1994 | 7576035 |
| hydrophobicity engineering to facilitate surface display of heterologous gene products on staphylococcus xylosus. | protein engineering has been employed to investigate the effect of specific amino acid changes on the targeting of heterologous proteins to the outer cell surface of the gram-positive bacterium staphylococcus xylosus. three different variants, corresponding to a 101 amino acid region of the major glycoprotein (g protein) of human respiratory syncytial virus (rsv), were generated in which multiple hydrophobic phenylalanine residues were either substituted or deleted. the different g protein fragm ... | 1995 | 7576540 |
| interleukin-1 alpha mediates the enhanced expression of intercellular adhesion molecule-1 in pulmonary epithelial cells infected with respiratory syncytial virus. | the mechanisms of virus-induced enhancement of intercellular adhesion molecule-1 (icam-1) expression in epithelial cells are unknown. in the present study, the effect of respiratory syncytial virus (rsv) infection on the expression of icam-1 in human pulmonary type ii-like epithelial (a549) cells was evaluated. conditioned rsv media (crsv) produced from growth of rsv in a549 cells induced a significant increase in the expression of icam-1. treatment of the cells with noninfectious crsv prepared ... | 1995 | 7576697 |
| [characteristics of monoclonal antibodies to rs virus in immunoenzyme and immunofluorescence techniques]. | according to the competitive elisa test data, new preparations of monoclonal antibodies (mab) to respiratory syncytial virus (rsv) differed in their blocking activity and they were directed to 3 different virus epitopes. mab 9c5 and mab 131-2a (cdc, atlanta) competed against each other strongly and they were directed to epitope f1a of rsv f-protein. mabs 8c5 and 10d8 showed a two-way blocking and were presumably topologically linked. mab 8b10 did not compete against other mabs. the fact that mab ... | 1995 | 7580416 |
| mucosal t cells recovered from mice after infection with respiratory syncytial virus display a memory/activation phenotype. | pgp-1 expression was studied as a marker of memory/activation on systemic and mucosal t cells of balb/c and c57bl/6 mice after infection with respiratory syncytial virus (rsv), using two-color dual fluorescence flow cytometry employing anti-l3t4 (cd4), anti-ly2 (cd8), and anti-pgp-1 (cd44) monoclonal antibodies. pgp-1 was expressed in relatively low densities on t cells of c57bl/6 mice, allowing differentiation of a dual population of pgp-1(10) and pgp-1hi t cells after antigenic stimulation in ... | 1995 | 7581721 |
| testing models predicting severity of respiratory syncytial virus infection on the picnic rsv database. pediatric investigators collaborative network on infections in canada. | to determine the sensitivity and specificity of published prognostic models to predict morbidity resulting from lower respiratory tract disease caused by respiratory syncytial virus in an independent pediatric population and to assess the accuracy of single risk factors in predicting adverse outcome. | 1995 | 7581752 |
| role of ecmo in the treatment of respiratory syncytial virus bronchiolitis: a collaborative report. | to report the collaborative experience of extracorporeal membrane oxygenation (ecmo) in the treatment of respiratory syncytial virus (rsv) bronchiolitis between april 1989 and january 1995. | 1995 | 7583613 |
| immunohistochemical analysis of cells infiltrating rous sarcoma virus-induced tumors in chickens. | the aim of our experiments was the analysis of peripheral blood lymphocytes (pbl) and tumor infiltrating lymphocytes (til) in chicken inbred congenic lines, cb line with regressing and cc line with progressing rsv-induced sarcomas. for serological analysis, monoclonal antibodies to cd4, cd8, tcr1, tcr2, mhc class i and class ii antigens were used. significant differences determined by flow cytometry in cd4+ and cd8+ populations of pbl between cb chickens with tumors in progressive phase and cc c ... | 1994 | 7589704 |
| the ser36-ser37 pair in hela nuclear protein p21/siir mediates ser/thr phosphorylation and is essential for rous sarcoma virus long terminal repeat repression. | phosphorylation of hela sii (or tfiis)-related nuclear protein p21/siir was demonstrated in transfected cos-1 cells. to test for a possible functional link between phosphorylation and the previously described rous sarcoma virus (rsv) long terminal repeat (ltr) repression (yeh, c.h., and shatkin, a.j. (1994) proc. natl. acad. sci. u.s.a. 91, 11002-11006), p21/siir mutants were constructed and assayed for phosphorylation level and effect on rsv ltr-driven chloramphenicol acetyltransferase (cat) re ... | 1995 | 7592688 |
| respiratory syncytial virus induces interleukin-10 by human alveolar macrophages. suppression of early cytokine production and implications for incomplete immunity. | respiratory syncytial virus (rsv) causes repeated infections thought to be due to an ineffective immune response. we examined the hypothesis that incomplete immunity may result, in part, from rsv-infected alveolar macrophage production of il-10 which can interfere with the production of immunoregulatory cytokines. we also assessed whether rsv induced the expression of the 2',5' oligoadenylate (2-5a)-dependent rnase l, an endoribonuclease involved in the antiviral activities of interferons. human ... | 1995 | 7593633 |
| the role of bronchodilators in the management of bronchiolitis: a clinical trial. | a randomized clinical trial was conducted on young children with bronchiolitis admitted to hospital with moderate illness to determine the efficacy of the bronchodilators salbutamol and ipratropium bromide, either as a single drug or in combination, given as a nebulized solution, compared with a normal saline placebo. eighty-nine patients, aged from 23 days to 11 months, were randomized into four groups, depending on administered drug or placebo, as follows: group 1--salbutamol (n = 20); group 2 ... | 1995 | 7598441 |
| hydrophobicity engineering to increase solubility and stability of a recombinant protein from respiratory syncytial virus. | site-directed mutagenesis has been employed to engineer the hydrophobic properties of a 101-amino-acid fragment from the human respiratory syncytial virus (rsv) major glycoprotein (g protein). when this protein was produced in escherichia coli, more than 70% of the gene product was found as inclusion bodies, and the product recovered from the soluble fraction was severely degraded. substitution of two cysteine residues for serine residues, did not significantly change the solubility or stability ... | 1995 | 7601124 |
| inhibition of herpes simplex virus type 1, respiratory syncytial virus and echovirus type 11 by peroxidase-generated hypothiocyanite. | the human mouth is an important route of viral transmission and evidence exists that human saliva can neutralize some viruses, e.g. herpes simplex type 1 (hsv-1) and human immunodeficiency virus (hiv) in vitro. however, little is known of the actual antiviral agents in saliva. we have analyzed how hypothiocyanite (hoscn/-oscn) ions, present in human saliva and generated by salivary peroxidase systems, affect the viability of three different types of viruses; hsv-1 (capable of inducing oral lesio ... | 1995 | 7605114 |
| respiratory syncytial virus (rsv) or rous sarcoma virus (rsv)? | | 1995 | 7606981 |
| antiviral activity of herpes simplex virus vectors expressing murine alpha 1-interferon. | mutant herpes simplex virus type i (hsv-1) vectors were engineered to express murine alpha 1 interferon (ifn) and assessed for their ability to inhibit the replication of challenge viruses in infection of monolayer cell cultures. the alpha 1 ifn gene was placed under control of the rous sarcoma virus (rsv) long terminal repeat (ltr) region in the thymidine kinase (tk) locus of both the wild-type hsv-1 strain kos and the replication-defective kos mutant dl20, in which both copies of the icp4 imme ... | 1995 | 7614249 |
| occurrence of respiratory syncytial virus subtypes in mexican infants with acute lower respiratory tract disease. | the occurrence of respiratory syncytial virus (rsv) subtypes in mexico city was studied by searching for the virus in nasopharyngeal aspirates of 82 infants, less than 12 months of age, hospitalized for acute lower respiratory tract disease with diagnosis of bronchitis, bronchiolitis, pneumonia and laryngotracheobronchitis. the presence of respiratory virus was determined by observation of cytopathic effect in cell monolayers; samples which produced a clear syncytia were considered positive and ... | 1995 | 7620276 |
| respiratory syncytial virus and influenza a infections in the hospitalized elderly. | respiratory syncytial virus (rsv) infections in the institutionalized elderly have been described; however, there is little information on the impact of rsv infection on community-dwelling elderly. the purpose of this study was to determine the relative numbers of hospitalizations associated with rsv infection and compare the clinical manifestations with influenza a infection. between november and april during 1989-1992, persons > or = 65 years old hospitalized with acute cardiopulmonary conditi ... | 1995 | 7622882 |
| oxidant tone regulates il-8 production in epithelium infected with respiratory syncytial virus. | respiratory syncytial virus (rsv) is an important respiratory pathogen that preferentially infects epithelial cells in the airway, and causes a local inflammatory response. although it has been previously demonstrated that rsv-infected airway epithelial produce cytokines, including interleukin-8 (il-8), which contributes to the inflammatory response, the regulation of this effect of rsv is unknown. to further characterize the mechanisms by which rsv infection triggers release of il-8, we first e ... | 1995 | 7626291 |
| a novel line of transgenic mice (rsv/ltr-bgh) expressing growth hormone in cardiac and striated muscle. | in order to further investigate the deleterious effects of gh overexpression, we generated a novel line of transgenic mice featuring stable and specific expression of bovine gh in the heart and striated muscle. a dna construct, containing a region with promoter activity from the long terminal repeat of rous sarcoma virus (rsv-ltr) and the entire structural gene of bovine gh (bgh), was microinjected by standard techniques in male pronuclei of fertilized mice eggs. transgenic mice expressed bgh mr ... | 1995 | 7627093 |
| [analysis of expression of the rsv-lacz-gene in transgenic embryos of the loach misgurnus fossilis l. during different variations of injection]. | several series of microinjection of the rsv-lacz gene (the escherichia coli beta-galactosidase gene under the control of the long terminal repeat of the raus sarcoma virus) into fertilized mud loach eggs were carried out. the expression of the transgene in 3-5 day-old fry was shown to depend neither upon the stage of fry development at which the rsv-lacz gene was introduced (early blastodisc, late blastodisc, 2-blastomere embryo) nor upon the region of transgene injection (blastodisc cytoplasm o ... | 1995 | 7635315 |
| sequential respiratory syncytial virus and cytomegalovirus pneumonia following bone marrow transplantation. | a 6-month-old child with familial hemophagocytic lymphohistiocytosis (fhl) experienced early sequential pneumonia due to respiratory syncytial virus (rsv) and cytomegalovirus (cmv) following bone marrow transplantation (bmt). the patient was deficient in natural killer (nk) cell activity (as found frequently in patients with fhl), and this risk factor may have played a major role in the concomitant infection by the two viral pathogens. rapid diagnostic methods for both viruses are essential and ... | 1995 | 7636507 |
| rna replication by respiratory syncytial virus (rsv) is directed by the n, p, and l proteins; transcription also occurs under these conditions but requires rsv superinfection for efficient synthesis of full-length mrna. | previously, a cdna was constructed so that transcription by t7 rna polymerase yielded a approximately 1-kb negative-sense analog of genomic rna of human respiratory syncytial virus (rsv) containing the gene for chloramphenicol acetyltransferase (cat) under the control of putative rsv transcription motifs and flanked by the rsv genomic termini. when transfected into rsv-infected cells, this minigenome was "rescued," as evidenced by high levels of cat expression and the production of transmissible ... | 1995 | 7637014 |
| comparison of rapid immunofluorescence procedure with testpack rsv and directigen flu-a for diagnosis of respiratory syncytial virus and influenza a virus. | a rapid immunofluorescence format requiring 20 min for completion was as effective as conventional indirect and direct immunofluorescence procedures for detecting respiratory syncytial virus and influenza a virus antigens in clinical specimens. rapid immunofluorescence was more sensitive than testpack rsv and comparable to directigen flu-a immunosorbent assays, which require 20 min for completion. | 1995 | 7650206 |
| interleukin-12 treatment during immunization elicits a t helper cell type 1-like immune response in mice challenged with respiratory syncytial virus and improves vaccine immunogenicity. | adjuvant effects of exogenous interleukin (il)-12 on induction of immune responses against respiratory syncytial virus (rsv) infection in mice were evaluated. giving recombinant il-12 at the time of immunization with a formalin-inactivated alum-precipitated rsv preparation resulted in significant reduction of virus replication in lungs 4 days after rsv challenge. intraperitoneal or intramuscular il-12 was effective when given at the time of immunization but not at the time of challenge. il-12 tr ... | 1995 | 7658066 |
| albuterol responsiveness in infants with respiratory failure caused by respiratory syncytial virus infection. | to assess the bronchodilator effect of inhaled albuterol in the acute stage of severe respiratory syncytial virus (rsv) infection. | 1995 | 7658285 |
| in vitro analysis of a primary, major histocompatibility complex (mhc)-restricted, cytotoxic t-lymphocyte response to avian leukosis virus (alv), using target cells expressing mhc class i cdna inserted into a recombinant alv vector. | the interaction between the major histocompatibility complex (mhc) and cytotoxic t lymphocytes (ctls) is an important component of the host's resistance to viral infections and tumor formation. in this study, an avian leukosis virus (alv) vector system, rcasbp, expressing mhc chicken class i (b-f) cdna was used to develop target cells expressing the chicken class i glycoproteins complexed with alv antigens on the cell surface. peripheral blood from chickens inoculated with alv was shown to conta ... | 1995 | 7666545 |
| self-assembly in vitro of purified ca-nc proteins from rous sarcoma virus and human immunodeficiency virus type 1. | the internal structural proteins of retroviruses are proteolytically processed from the gag polyprotein, which alone is able to assemble into virus-like particles when expressed in cells. all gag proteins contain domains corresponding to the three structural proteins ma, ca, and nc. we have expressed the ca and nc domains together as a unit in escherichia coli, both for rous sarcoma virus (rsv) and for human immunodeficiency virus type 1 (hiv-1). we also expressed a similar hiv-1 protein carryin ... | 1995 | 7666550 |
| incidence of mycoplasma pneumoniae, chlamydia trachomatis, and viral infections in pneumonia cases under six months of age, bangkok, thailand. | the incidence of infections by mycoplasma pneumoniae, chlamydia trachomatis and respiratory viruses was investigated in 76 pneumonic patients aged under 6 months who attended ramathibodi and siriraj hospitals in bangkok during two study periods. m. pneumoniae infection was not found in any case from either hospital by serological diagnosis. by the isolation method, c. trachomatis infection was found in 7(16.7%) of 42 patients from ramathibodi hospital and 5(21.7%) of 23 patients from siriraj hos ... | 1994 | 7667709 |
| influence of expression and cis-acting sequences from avian leukosis viruses (alvs) on stability of (alv)-based retrovirus vectors. | defective avian leukosis virus (alv)-based vectors expressing the neo and lacz genes were constructed under the control of cis-acting elements originated from 4 avian retroviruses: avian erythroblastosis virus (aev), rous associated viruses 1 (rav-1) and 2 (rav-2), and the schmidt ruppin strain of rous sarcoma virus subgroup d (sr-rsv-d). we used these vectors to study the long-term stability of beta-galactosidase expression (encoded by the lacz gene) in a permanent cell line from quail fibrobla ... | 1995 | 7671000 |
| differentiation of pc12 cells by infection with an hsv-1 vector expressing nerve growth factor. | we have developed an hsv-1 vector with mutations in the viral ie 3 and vp16 genes that expresses mouse beta nerve growth factor (ngf) from a latency associated transcript (lat) promoter modified by insertion of a rous sarcoma virus (rsv) enhancer. the backbone double mutant vector has reduced cytotoxicity compared with a single mutant deleted for ie 3 and is able to express the reporter luciferase gene in rat pheochromocytoma (pc12) cells at low but relatively stable levels in vitro. intracellul ... | 1995 | 7671108 |
| three antigenic variant groups in human respiratory syncytial virus subgroup b isolated in japan. | nineteen hybridomas producing monoclonal antibodies (mabs) against the structural proteins of strain 58-17, a subgroup b field strain of respiratory syncytial virus (rsv) isolated in japan, were obtained by fusion of x63 myeloma cells with spleen cells from balb/c mice immunized with the virus-infected hep-2 cells. seven clones were found to produce antibodies against the fusion protein (f), five against the large glycoprotein (g), five against the nucleoprotein (np) and two against the 22k prot ... | 1993 | 7678192 |
| profound differences in potassium current properties of normal and rous sarcoma virus-transformed chicken embryo fibroblasts. | the membrane currents of chicken embryo fibroblasts (cefs) transformed by rous sarcoma virus (rsv) were compared with the currents of their nontransformed counterparts by using the whole-cell patch-clamp technique. in nontransformed cefs, the main membrane current is a delayed outward k+ current that is sensitive to tetraethylammonium ion but insensitive to 4-aminopyridine. this k+ current is almost independent of the intracellular ca2+ concentration and becomes completely inactivated at positiv ... | 1993 | 7682700 |
| immunization of mice with vaccinia virus-m2 recombinant induces epitope-specific and cross-reactive kd-restricted cd8+ cytotoxic t cells. | the m2 protein of respiratory syncytial virus (rsv) is a protective antigen in h-2d, but not h-2b or h-2k mice. none of the other rsv proteins, excluding the surface glycoproteins that induce neutralizing antibodies, is protective in mice bearing these haplotypes. thus, the m2 protein stands alone as a nonglycoprotein-protective antigen of rsv. the m2 protein is a target for murine kd-restricted cytotoxic t lymphocytes (ctls), and the resistance induced by infection with a vaccinia virus-rsv m2 ... | 1993 | 7685408 |
| alterations of the three short open reading frames in the rous sarcoma virus leader rna modulate viral replication and gene expression. | the rous sarcoma virus (rsv) leader rna has three short open reading frames (orf1 to orf3) which are conserved in all avian sarcoma-leukosis retroviruses. effects on virus propagation were determined following three types of alterations in the orfs: (i) replacement of aug initiation codons in order to prohibit orf translation, (ii) alterations of the codon context around the aug initiation codon to enhance translation of the normally silent orf3, and (iii) elongation of the orf coding sequences. ... | 1993 | 7685415 |
| identification of t-cell epitopes adjacent to neutralizing antigenic domains on the fusion protein of respiratory syncytial virus. | f glycoprotein has been identified as an important target structure in the immunological response following respiratory syncytial virus (rsv) infection. two sequential b epitopes corresponding to amino acids 200 to 225 and 255 to 278 have already been defined with anti-rsv rabbit serum. the t helper response to peptides which belong to these sequences was investigated in this study. proliferative t-cell responses to these peptides were analysed in balb/c mice (h-2d) and others strains: sjl (h-2s ... | 1993 | 7685537 |
| decrease in coupling of gs in v-src-transformed nih-3t3 fibroblasts: possible involvement of tyrosine phosphorylation of gs by pp60v-src. | in rous sarcoma virus (rsv)-transformed nih-3t3 fibroblasts expressing pp60v-src as tyrosine protein kinase, isoproterenol-stimulated camp accumulation was much lower than in normal cells. the reduction in v-src-transformed cells seemed to be mainly due to a decrease in the number of beta 2-adrenoceptors. when the membranes were phosphorylated with atp, however, the binding affinity of isoproterenol to beta 2-adrenoceptors was reduced in transformed cell membranes by 34% compared to that in norm ... | 1993 | 7686735 |
| morphological transformation, tumorigenicity and src-specific cytotoxic t-lymphocyte-mediated tumor immunity induced by murine 3t3 cells expressing src oncogenes encoding novel non-myristylated n-terminal domains. | we previously reported the development of a src-specific tumor regression system in chickens in which preinfection with rasv1702, a mutant of rous sarcoma virus (rsv) encoding non-myristylated src product with a novel n-terminal domain, results in the immune suppression of challenge tumors induced by rsv. in order to adapt this system to the mouse, we have developed nih3t3 and balb/c3t3 (b3t3) cell lines that express 1702src, v-src, c-src, and other src variants, either by transfection or by inf ... | 1993 | 7692370 |
| human corneal epithelial primary cultures and cell lines with extended life span: in vitro model for ocular studies. | to develop an in vitro model of human corneal epithelium that can be propagated in serum-free medium that is tissue specific, species specific, and continuously available. | 1993 | 7693609 |
| intranasal monoclonal immunoglobulin a against respiratory syncytial virus protects against upper and lower respiratory tract infections in mice. | the role of secretory antibody in protection against respiratory syncytial virus (rsv) infection was examined by using monoclonal immunoglobulin a (iga) antibody for intranasal passive immunization of mice. eight anti-rsv iga hybridomas were produced by fusing myeloma cells with lung lymphocytes from rsv-immunized mice. five iga antibodies recognized rsv strains of both the a and the b subgroups, and two of these neutralized virus in a plaque reduction assay. monoclonal iga antibody hnk20, which ... | 1994 | 7695263 |
| respiratory syncytial virus (rsv) infection in preterm infants and the protective effects of rsv immune globulin (rsvig). respiratory syncytial virus immune globulin study group. | to evaluate the safety and efficacy of respiratory syncytial virus immune globulin (rsvig) in the prevention of severe respiratory syncytial virus (rsv) lower respiratory tract infection (lrti) in infants born prematurely with or without bronchopulmonary dysplasia (bpd). | 1995 | 7700741 |
| asthma and immunoglobulin e antibodies after respiratory syncytial virus bronchiolitis: a prospective cohort study with matched controls. | to study the occurrence of bronchial obstructive symptoms and immunoglobulin (ig) e antibodies after respiratory syncytial virus (rsv) bronchiolitis in infancy. previous studies of this subject have mostly been retrospective or without controls, or the controls have not been followed prospectively. | 1995 | 7700748 |
| hyperimmune globulins in prevention and treatment of respiratory syncytial virus infections. | respiratory syncytial virus (rsv) is an important community and nosocomial respiratory pathogen for infants and young children. rsv causes especially severe disease in the prematurely born or those with chronic cardiopulmonary diseases. elderly persons and those with t-cell deficiencies, such as bone marrow transplant recipients, are also at high risk for serious lower respiratory tract infections. to date, prevention of rsv infections by vaccination has proven elusive and no preventive drugs ex ... | 1995 | 7704893 |
| evolution of subgroup a respiratory syncytial virus: evidence for progressive accumulation of amino acid changes in the attachment protein. | the variability of the attachment (g) proteins of 48 subgroup a isolates of respiratory syncytial virus (rsv) isolated over 38 years has been examined. nucleotide sequences of two variable regions of the g protein genes were determined following amplification by pcr. the isolates showed temporal rather than geographical clustering, and there was evidence for progressive accumulation of amino acid changes at an average rate of approximately 0.25% per year estimated over the entire protein. the co ... | 1995 | 7707517 |
| rsv and the high-risk infant. | respiratory syncytial virus (rsv) is a common cause of lower respiratory tract infection in infants and young children. it is most devastating to infants with certain underlying conditions such as cardiovascular and pulmonary disease, immunosuppressed infants, and those with a history of prematurity. management of the high-risk infant with rsv is a complex process and requires an understanding of epidemiology, mode of transmission, pathophysiology, clinical manifestations, diagnosis, medical tre ... | 1994 | 7708456 |
| the first and third uorfs in rsv leader rna are efficiently translated: implications for translational regulation and viral rna packaging. | rous sarcoma virus (rsv) rna leader contains three short upstream open reading frames. we have shown recently that both uorfs 1 and 3 influence in vivo translation of the downstream gag gene and are involved in the virus rna packaging process. in this report, we have studied the translational events occurring at the upstream augs in vivo. we show that (i) the first and third augs are efficient translational initiation sites; (ii) ribosomes reinitiate efficiently at aug3; and (iii) deletions in t ... | 1995 | 7708504 |
| inhibition of replication of human respiratory syncytial virus by 6-diazo-5-oxo-l-norleucine. | the effect of 6-diazo-5-oxo-l-norleucine (l-don), a glutamine analog, on rsv replication was studied. at a concentration of 0.01 mm l-don, 99% of rsv replication in treated cv-1 cells was inhibited. at this concentration of l-don, the level of cellular protein synthesis was identical to untreated control cells. trypan blue staining revealed that all the cells remained viable even at concentrations of l-don as high as 10 mm. in addition, l-don added as late as 24 h post infection can effectively ... | 1994 | 7710273 |
| a bk virus episomal vector for constitutive high expression of exogenous cdnas in human cells. | a bk virus (bkv) episomal vector (prpneocmv) was constructed for expression of cdnas under control of the cytomegalovirus (cmv) immediate-early promoter. transfection of prpneocmv for expression of the chloramphenicol acetyltransferase (cat) gene in several human cell lines showed that the cmv promoter is more efficient than the hiv-1 and rsv ltrs in directing gene expression from episomal vectors. in 293 human cells prpneocmv/cat is twenty times more active in cat expression than the well known ... | 1995 | 7710358 |
| optimization of pcr and automated sequencing of clinical isolates of respiratory syncytial virus. | a streamlined protocol was developed to carry out nucleotide sequence analysis on pcr products obtained from regions of the rna genome of respiratory syncytial virus (rsv). whole cell rna extracted from tissue culture cells inoculated with rsv-infected nasopharyngeal aspirates was used as a template for single-tube cdna synthesis and pcr amplification of specific regions of the rsv fusion (f) protein gene. the products were then purified using sephacryl spin columns and digested using lambda-exo ... | 1994 | 7714055 |
| routine diagnosis of seven respiratory viruses and mycoplasma pneumoniae by enzyme immunoassay. | a composite eia, using 8-well microstrips, was used for the rapid detection of seven respiratory viruses and m. pneumoniae. the viruses included influenza a and b, parainfluenza 1, 2 and 3, adenovirus and respiratory syncytial virus. during the 61 month period--june 1988 to june 1993--17326 respiratory specimens, submitted from three states, were tested by this eia. the specimens were mainly from a paediatric population (hospitals and private physicians). rsv was the predominant virus detected, ... | 1994 | 7714062 |
| nonstructural protein 2 (ns2) of respiratory syncytial virus (rsv) detected by an antipeptide serum. | the human respiratory syncytial virus (rsv) is often associated with airway obstruction and is suspected to induce bronchial hyperreactivity. interactions of viral proteins with cellular components may be responsible for epithelial damage leading to bronchial hyperreactivity. in this study, we describe the localization of the 14.7-kd nonstructural protein 2 (ns2) in rsv-infected cells. the detection of ns2 was performed using antipeptide antibodies elicited against amino acids 109-123 of the pre ... | 1995 | 7716351 |
| tryptase and ige concentrations in the respiratory tract of infants with acute bronchiolitis. | it has been proposed that a specific ige response contributes to the immunopathology of acute respiratory syncytial virus (rsv) bronchiolitis but previous work has been difficult to replicate. indirect evidence that might support this contention was sought by measuring total ige concentrations in bronchoalveolar lavage (bal) samples obtained from intubated infants and by attempting to detect mrna for ige in cells obtained from both the upper and lower respiratory tract. evidence of significant m ... | 1995 | 7717746 |
| a bcl-2-related gene is activated in avian cells transformed by the rous sarcoma virus. | the oncoprotein p60v-src encoded by the rous sarcoma virus (rsv) genome is the prototype of non-receptor tyrosine kinases. more than 50 targets of p60v-src have been described to date. however, the precise mechanisms of rsv transformation remain to be elucidated. here, we present the study of a new v-src-activated gene, nr-13, which encodes a protein identified as a new member of the bcl-2 family. this protein is localized in the membrane with a pattern already observed with bcl-2. in quail embr ... | 1995 | 7729415 |
| characterization of the promoter of human pulmonary surfactant protein b gene. | pulmonary surfactant protein b (sp-b) is required for normal surfactant function and for survival at birth. to further study sp-b gene expression, we sequenced genomic clones and examined promoter activity of sp-b dna fragments by transient transfection. a plasmid construct containing human sp-b fragment -1039/+431 linked to chloramphenicol acetyltransferase (cat) reporter gene was readily expressed in h441 cells, which are derived from a human lung adenocarcinoma, but was < 4% as active in hep ... | 1995 | 7733308 |
| sequelae of respiratory syncytial virus infections. a role for intervention studies. | the association between respiratory syncytial virus (rsv) infections in infancy and respiratory abnormalities later in life has been attributed both to a direct effect of the infection itself and to an inherent susceptibility. observational studies do not allow a rigorous test of these hypotheses. respiratory syncytial virus infection is universal in the first years of life and no uninfected control group exists. randomized, controlled trials using new prophylactic agents such as vaccines or spe ... | 1995 | 7735633 |
| [in vitro and in vivo study of the activity of secreted alkaline phosphatase mrna ribozyme gene]. | a ribozyme was constructed of the catalytic domain of tobacco ringspot virus satellite rna and flanking sequences complementary to the target, secreted alkaline phosphatase (seap) mrna. the ribozyme specifically cleaved the substrate in vitro; compared with that constant temperature. the relationship between specific endoribonuclease activity of the ribozyme and mg2+ concentration was shown. the ribozyme was active in 293 cell line which was cotransfected with plasmids carrying the seap gene und ... | 1994 | 7739597 |
| transplacental transfer of antibodies to some respiratory viruses. | a group of 200 paired mother-newborn sera (umbilical sera) were used for the determination, by hemagglutination inhibition test of antibody levels for parainfluenza virus types 1, 2 and 3 and, by elisa test of class igg antibodies for respiratory syncytial virus (rsv). in 54 paired sera the rsv antibodies also were determined in igg subclasses and in 4 paired sera rsv protein antibodies were determined by western-blot in igg class and igg subclasses. parainfluenza virus antibodies were found to ... | 1994 | 7740985 |
| respiratory syncytial virus (rsv) antibodies in different immunoglobulin classes in small children. | percent of rsv antibodies in the individual immunoglobulin classes and igg subclasses was determined by elisa and the immune response to rsv infection was evaluated in children 0-2 years of age. in the first 6 months percent of sera containing igg-rsv declined to reach minimal in the 7th-9th month, then it rose to 100% in children 2 years of age. percent of positive igm-rsv and iga-rsv gradually increased with age. the ige antibodies occurred in highest percent (20%) in the youngest children, an ... | 1994 | 7740986 |
| the immune response of small children by antibodies of different classes to respiratory syncytial virus (rsv) proteins. | a group of 18 paired sera, obtained from rsv-infected children were assayed by western-blot for antibodies for rsv proteins in the immunoglobulin classes igg, igm, iga and ige and in the igg subclasses. the igg antibodies for the protein g were found in almost half of the sera tested, while in the other immunoglobulin classes the antibodies occurred in a much lower number of the sera. the antibodies for protein f1 were detected first of all in the igg class and subclasses. the antibodies for pro ... | 1994 | 7740987 |
| a cold-passaged, attenuated strain of human respiratory syncytial virus contains mutations in the f and l genes. | in previous studies, a mutant (cp-rsv) of the rsv a2 strain derived from 52 serial cold passages in bovine embryonic tissue culture was highly attenuated in seropositive adults and children but caused upper respiratory tract disease in seronegative infants. we investigated the genetic basis for this attenuation phenotype by comparing the complete genomic rna sequence of this virus with the published sequence of strain a2 as well as with that of its unattenuated wild-type parent (hek-7) virus. rn ... | 1995 | 7747420 |
| morphological characters and disturbance of myofibril assembly in avian myoblasts fused with hvj (sendai virus). | we have been studying myogenic differentiation, especially the mechanism of myotube formation, using quail myoblasts transformed with a temperature-sensitive mutant of rous sarcoma virus, qm-rsv cells. myogenic differentiation of qm-rsv cells can be controlled by the incubation temperature. when cultured at 35.5 degrees c, a permissive temperature for the virus, the cells proliferate but do not differentiate, whereas, when incubated at 41.0 degrees c, a non-permissive temperature, they proceed t ... | 1995 | 7750523 |
| [respiratory tract infection and respiratory syncytial virus in young infants]. | we investigated the role of respiratory syncytial virus (rsv) in respiratory tract infections of up-to-3-months old infants. this prospective study was carried out from april 1993 to march 1994. detection of rsv antigen in nasopharyngeal specimens was done by enzyme immunoassay using testpack rsv at our out patient clinic. during this study period, 65 young infants with respiratory tract infection visited our clinic. seventeen patients (26%) were diagnosed as having rsv infection. fifteen out of ... | 1995 | 7751737 |
| a prototype recombinant vaccine against respiratory syncytial virus and parainfluenza virus type 3. | we have produced a genetically-engineered chimeric protein composed of the external domains of the respiratory syncytial virus (rsv) fusion (f) protein and the parainfluenza virus type 3 (piv-3) hemagglutinin-neuraminidase (hn) protein in insect cells using the baculovirus expression system. the yield of the soluble chimeric frsv-hnpiv-3 protein could be increased approximately 2-fold by using trichoplasia ni (high five) insect cells in place of spodoptera frugiperda (sf9) for expression. the ch ... | 1994 | 7765021 |
| nosocomial viral pneumonia in the intensive care unit. | the role viruses play in nosocomial icu pneumonias is not well documented except for outbreaks of influenza and respiratory syncytial virus (rsv) infections. clinically, viral pneumonias are difficult to differentiate from bacterial pneumonias, and most routine diagnostic tests are able to diagnose viral pathogens. therefore, the incidence of viral pneumonias is almost certainly underestimated. the likelihood of a viral pneumonia is increased if the patient is not responding to antimicrobial age ... | 1995 | 7768085 |
| topical immunoprophylaxis of respiratory syncytial virus (rsv)-challenged mice with rsv-specific immune globulin. | the modulatory effects of passive antibody on viral replication, illness, and immunity were investigated in a murine model of respiratory syncytial virus (rsv) infection. nasally administered rsv-specific immune globulin (rsvig) given prophylactically in a large volume (100 microl) reduced illness and rsv replication in lung after primary challenge. however, passive antibody treatment diminished the host antibody response to primary rsv infection. this resulted in greater susceptibility to reinf ... | 1995 | 7769280 |
| formulation of the purified fusion protein of respiratory syncytial virus with the saponin qs-21 induces protective immune responses in balb/c mice that are similar to those generated by experimental infection. | the feasibility of employing a vaccine composed of the purified fraction 21 of quillaja saponaria (qs-21) and the fusion (f) protein of respiratory syncytial virus (rsv) to induce protective immune responses in the lower respiratory tract of balb/c mice was examined. our goal was to compare local and systemic immune responses with those induced following immunization with the protein adsorbed to aluminium hydroxide (f/aloh) adjuvant or by experimental infection. sera from mice vaccinated with th ... | 1995 | 7793137 |
| current approaches to the development of vaccines against disease caused by respiratory syncytial virus (rsv) and parainfluenza virus (piv). a meeting report of the who programme for vaccine development. | the paramyxoviruses respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) are the two most common agents of severe lower respiratory tract disease in infants and children throughout the world. rsv causes yearly epidemics of bronchiolitis and pneumonia in infants and young children, while piv3 is a common cause of bronchiolitis, pneumonia and croup. together these two agents account for up to 30% of all hospitalizations of infants and young children for respiratory tract disease ... | 1995 | 7793140 |
| respiratory syncytial virus and bronchiolitis. | viral bronchiolitis is a common world-wide disease of infants and children resulting in respiratory failure and occasionally death. the major underlying pathophysiology is airway inflammation of peripheral airways and airway hyperresponsiveness to bronchoprovocation. management is primarily prevention through strict hand washing and avoidance of exposures during respiratory seasons, especially in small infants who have underlying heart or lung disease. careful supportive therapy, including fluid ... | 1995 | 7793284 |
| safety and bioequivalency of three formulations of respiratory syncytial virus-enriched immunoglobulin. | respiratory syncytial virus (rsv) causes serious illness (lower respiratory illness) in preterm infants. rsv antibody-enriched immunoglobulin (rsvig) that was lyophilized (lyo) protected against rsv lower respiratory illness. the food and drug administration now requires an additional viral inactivation step (vi). we compared lyo, lyo-vi, and a more convenient liquid rsvig (liq-vi) in 30 preterm infants (median age, 7 months; median weight, 5.4 kg). infants were randomized to receive lyo (n = 10 ... | 1995 | 7793870 |
| a cis-acting element in rous sarcoma virus long terminal repeat required for promoter repression by hela nuclear protein p21. | hela cell basic nuclear protein (p21), which represses rous sarcoma virus long terminal repeat (rsv ltr) promoter activity, diminished v-src expression and the appearance at permissive temperature of the transformed phenotype in tsrsvla23 rat-1, a cell line transformed with a temperature-sensitive mutant of rsv. nuclear run-on analyses using cos-1 cells cotransfected with p21 cdna and chloramphenicol acetyltransferase reporter indicated that p21 inhibits transcription initiation by targeting a r ... | 1995 | 7797584 |
| protective and disease-enhancing immune responses to respiratory syncytial virus. | the national institutes of health, centers for disease control and prevention, and world health organization jointly sponsored a workshop on protective and disease-enhancing immune responses to respiratory syncytial virus (rsv). the primary purpose of the meeting was to discuss protective and disease-enhancing immune responses to rsv in the context of opportunities and barriers to the development of rsv vaccines. although both live attenuated and subunit vaccines have been developed, it is not y ... | 1995 | 7798649 |
| detection of respiratory syncytial virus by rna-polymerase chain reaction and differentiation of subgroups with oligonucleotide probes. | the polymerase chain reaction (rna-pcr) was used for specific detection of respiratory syncytial virus (rsv) genomes in clinical specimens. a set of primers was selected from conserved regions of the 1b and n genes for detection of both subgroups. the primers were found to be rsv specific, all rsv strains generated a 218 bp product, and no rsv specific amplified product was obtained when nucleic acids from a variety of micro-organisms from the respiratory tract were subjected to the rna-pcr. we ... | 1994 | 7798890 |
| epidemiology of viral infections and evaluation of the potential benefit of om-85 bv on the virologic status of children attending day-care centers. | viral investigations were performed during 4 winter seasons (88/89, 89/90, 92/93, 93/94) in children attending day-care centers (dccs) in the rhône département in eastern france. over the total observation period of 4 winter seasons, 780 children were screened with a nasal swab for the presence of viruses. of those, 230 (29.5%) had a positive viral culture. the viruses identified were respiratory syncytial virus (rsv), influenza a and b virus, parainfluenza virus, coronavirus, rhinovirus, adenov ... | 1994 | 7800968 |
| acute respiratory tract infection in daycare centers for older persons. | to evaluate the rate of specific pathogens and clinical syndromes associated with acute respiratory tract infections (arti) in frail older persons attending daycare. | 1995 | 7806736 |
| respiratory syncytial virus infections in human beings and in cattle. | respiratory syncytial virus (rsv) causes yearly outbreaks of respiratory disease in human beings and cattle all over the world. most severe human respiratory syncytial virus (hrsv)-associated disease is observed in children less than 1 year of age while most severe bovine respiratory syncytial virus (brsv)-associated disease is observed in calves less than 6 months of age. two subgroups of hrsv have been identified. the existence of two subgroups of brsv has been repeatedly suggested but is not ... | 1994 | 7806887 |
| safety and immunogenicity of a subunit respiratory syncytial virus vaccine in children 24 to 48 months old. | a subunit vaccine for respiratory syncytial virus (rsv) consisting of purified fusion glycoprotein (designated pfp-1) was tested in children 24 to 48 months old. two doses of 20 micrograms (n = 13) and 50 micrograms (n = 10) were compared with a saline (n = 24) placebo control group. local and systemic reactions, reported within 96 hours postvaccination, were mild, transient, and did not differ significantly from the control cohort. long term follow-up through at least one, and in some cases two ... | 1994 | 7808848 |
| development of nested pcr assays for detection of bovine respiratory syncytial virus in clinical samples. | two nested pcr assays were developed for the detection of bovine respiratory syncytial virus (brsv). primers were selected from the gene encoding the f fusion protein (pcr-f) and the gene encoding the g attachment protein (pcr-g). biotinylated oligonucleotide probes, termed f and g, were selected for the hybridization of the respective pcr products. the sensitivities of the pcr-f and pcr-g assays were similar, both detecting 0.1 tissue culture infective dose of the virus. the pcr-f assay amplifi ... | 1994 | 7814551 |
| cytotoxic t cells specific for a single peptide on the m2 protein of respiratory syncytial virus are the sole mediators of resistance induced by immunization with m2 encoded by a recombinant vaccinia virus. | we have studied the immunobiology of respiratory syncytial virus (rsv), a major cause of respiratory tract morbidity in children. as part of these studies, it was previously found that immunization of balb/c (h-2d) mice with a recombinant vaccinia virus (rvv) which encoded the m2 protein of rsv provided complete protection against infection with rsv. this protection was transient and associated with m2-specific cd8+ t-cell (tcd8+) responses. in this study, we used two approaches to demonstrate t ... | 1995 | 7815502 |
| neutralization of respiratory syncytial virus after cell attachment. | little is known about the mechanisms of antibody-mediated neutralization of respiratory syncytial virus (rsv) which causes recurrent infections in human despite the virtually universal presence of neutralizing serum antibodies. human serum neutralization titers showed strong correlation with post-cell-attachment neutralizing titers for both rsv-convalescent sera and control sera but showed less strong correlation with cell-attachment blocking titers. neutralization was effective for the first 60 ... | 1995 | 7815504 |
| [development of pcr tests for the detection of bovine herpesvirus-1, bovine respiratory syncytial viruses and pestiviruses]. | the development of pcr assays for detection of bhv-1, brsv, bvdv and another pestiviruses is summarized. a polymerase chain reaction assay based on primers selected from the viral gi glycoprotein gene detected 3 fg pure bhv-1 dna, 0.1-1.0 tcid50 or a single infected cell. no amplification was observed with dna from bhv-2, bhv-3, bhv-4, ohv-1 or ohv-2. however, a fragment of the correct size (468 bp) was amplified using dna from herpesviruses isolated from reindeer, red deer and goat. the pcr ass ... | 1994 | 7817501 |
| comparison of various transport media for viability maintenance of herpes simplex virus, respiratory syncytial virus, and adenovirus. | seven viral transport media (vtm) were compared for effectiveness in preserving the infectivity of herpes simplex virus (hsv), respiratory syncytial virus (rsv), and adenovirus. the media tested were richards viral transport, sucrose-phosphate-glutamate (spg), virocult, hh medium, tryptose phosphate broth, cell culture medium, and bartel's viral transport. two laboratory strains of hsv (mcintyre and 333) and two clinical isolates (a0301 and a0386), comprising two hsv-1 types and two hsv-2 types, ... | 1994 | 7820992 |
| 3-cyclopentyl-1-adamantanamines and adamantanemethanamines. antiviral activity evaluation and convulsions studies. | the synthesis of 3-cyclopentyl-1-adamantanamines and adamantanemethanamines and some of their thioureas is described. the antiviral activity examination of these compounds indicated that some of them inhibited respiratory syncytial virus (rsv) infection at concentrations that were slightly (up to 5-fold) lower than the cytotoxic concentration. behavioral and convulsions studies of the above mentioned amines, in mice, did not show any dopaminomimetic activity and argue in favor of the existence o ... | 1994 | 7826470 |
| treatment of respiratory syncytial virus infections in children. | treatment of the infections caused by the respiratory syncytial virus (rsv) has varied largely in different centres. recently, however, management practices have become more clear based on a number of studies. an infant with rsv bronchiolitis should be hospitalized in case of insufficient oxygenation, as measured by pulse oximetry, and additional oxygen should be supplied. mist treatment and physiotherapy are not beneficial. bronchodilators seem to be the drug of choice in most infants with bron ... | 1994 | 7826594 |
| activities and substrate specificity of the evolutionarily conserved central domain of retroviral integrase. | the retroviral integrase (in) is a virus-encoded enzyme that is essential for insertion of viral dna into the host chromosome. in order to map and define the properties of a minimal functional domain for this unique viral enzyme, a series of n- and c-terminal deletions of both rous sarcoma virus (rsv) and human immunodeficiency virus (hiv) ins were constructed. the rsv in deletion mutants were first tested for their ability to remove two nucleotides from the end of a substrate representing the t ... | 1995 | 7831800 |