| expression of a functional nad-reducing [nife] hydrogenase from the gram-positive rhodococcus opacus in the gram-negative ralstonia eutropha. | the actinomycete rhodococcus opacus mr11 harbors a bidirectional nad-reducing [nife] hydrogenase (sh). this cytoplasmic enzyme is composed of two heterodimeric modules which catalyze distinct enzymatic activities. the hydrogenase moiety mediates h(2):benzyl viologen oxidoreductase activity and the fmn-containing diaphorase module displays nadh:benzyl viologen oxidoreductase activity. the sh of rh. opacus resembles [nife] hydrogenases present in strains of the proteobacterium ralstonia eutropha a ... | 2002 | 11807565 |
| pulmonary infection caused by rhodococcus equi in hiv-infected patients: report of four patients from northern thailand. | we report four human immunodeficiency virus (hiv)-infected patients (3 men and one woman, average age, 34.3 years) with pulmonary infection (two with pneumonia and two with lung abscess) caused by rhodococcus equi. these patients, who presented with fever and productive cough, were admitted to nakornping hospital in northern thailand. chest roentgenograms showed pulmonary infiltration and/or cavitary lesions. their conditions were poor because of severe anemia, and transfusion was necessary in t ... | 2000 | 11810571 |
| a cycle of enzymatic reactions that behaves like a neuronal circuit. | a cycle of four enzymatic reactions with repression or allosteric inhibition of the enzymes has been proposed by analogy to a neural oscillator. the system is analysed in a situation remote from full symmetry. asymmetry has been introduced by treating one of the reagents as a reservoir substance with constant concentration and having essentially different rate constants for forward and backward reactions. it is demonstrated that, for certain values of parameters, the system can work as a strengt ... | 2002 | 11812176 |
| metabolic engineering of indene bioconversion in rhodococcus sp. | we have applied the methodology of metabolic engineering in the investigation of the enzymatic bioreaction network in rhodococcus sp. that catalyzes the bioconversion of indene to (2r)-indandiol suitable for the synthesis of cis-1-amino-2-indanol, a precursor of the hiv protease inhibitor, crixivan. a chemostat with a novel indene air delivery system was developed to facilitate the study of steady state physiology of rhodococcus sp. 124. prolonged cultivation of this organism in a continuous flo ... | 2001 | 11816813 |
| dna-based and culture-based characterization of a hydrocarbon-degrading consortium enriched from arctic soil. | a hydrocarbon-degrading consortium was enriched from fuel-contaminated soil from the northeastern tip of ellesmere island (82 degrees 30'n, 62 degrees 19'w). the enrichment culture was grown on jet a-1 fuel at 7 degrees c. bacterial 16s rna gene (rdna) fragments were amplified by polymerase chain reaction (pcr) from members of the above consortium and cloned into a plasmid vector. partial sequences (approximately 500 bp) were determined for 29 randomly selected rdna clones. the majority of seque ... | 2001 | 11822837 |
| comparison of the emulsion characteristics of rhodococcus erythropolis and escherichia coli soxc-5 cells expressing biodesulfurization genes. | biodesulfurization of fuel oils is a two-phase (oil/water) process which may offer an interesting alternative to conventional hydrodesulfurization due to the mild operating conditions and reaction specificity afforded by the biocatalyst. for biodesulfurization to realize commercial success, a variety of process considerations must be addressed including reaction rate, emulsion formation and breakage, biocatalyst recovery, and both gas and liquid mass transport. this study evaluates emulsion form ... | 2002 | 11822905 |
| chemostat approach for the directed evolution of biodesulfurization gain-of-function mutants. | chemostat enrichment is a classical microbiological method that is well suited for use in directed-evolution strategies. we used a two-phase sulfur-limited chemostat to select for gain-of-function mutants with mutations in the biodesulfurization (dsz) system of rhodococcus erythropolis igts8, enriching for growth in the presence of organosulfur compounds that could not support growth of the wild-type strain. mutations arose that allowed growth with octyl sulfide and 5-methylbenzothiophene as sol ... | 2002 | 11823208 |
| [alive microorganism in the workplace ambient air in plants disposing communal waste]. | this paper presents the results of the identification and determination of alive microorganisms in the air samples collected from the breath zone of workers employed in the waste collection and disposal. samples were taken during waste collection and in the waste reloading, sorting and composting plants. in addition, samples were taken at stationary sites: in the city and inside flats. different kinds of species of gram-positive bacteria (micrococcus, staphylococcus, enterococcus, bacillus, list ... | 2001 | 11828848 |
| enhancement of enzyme activity and enantioselectivity via cultivation in nitrile metabolism by rhodococcus sp. cgmcc 0497. | racemic 2-phenylpropionitrile was resolved enantioselectively by nitrile-converting enzymes in cells of rhodococcus sp. cgmcc 0497 to s-(+)-2-phenylpropionic acid and r-(-)-2-phenylpropionamide. by optimization of the culture conditions, great enhancement of enzyme activity and enantioselectivity was achieved. furthermore, the relationship between cell-growth periodicity and enzyme accumulation was studied; the addition of inducer was delayed by 1 day and the reaction was further improved. this ... | 2002 | 11834131 |
| rhodococcus equi pneumonia: highly active antiretroviral therapy helps but does not cure lung infection. | | 2002 | 11834975 |
| medical management of pneumonia caused by rhodococcus equi in a renal transplant recipient. | rhodococcus equi is an animal pathogen that occasionally causes opportunistic infections in immunocompromised patients. the most common clinical picture is one of necrotizing pneumonia with a tendency toward cavitation and the formation of abscesses. we report a case of pneumonia caused by r equi in a renal transplant patient. an excellent response was shown to antibiotic treatment. symptoms regressed, and the progressive disappearance of the lesion was confirmed on follow-up computed tomography ... | 2002 | 11840398 |
| rapid immunohistochemical detection of rhodococcus equi in impression smears from affected foals on postmortem examination. | the first objective of this study was to develop an immunohistochemical procedure for rapid detection of rhodococcus equi in impression smears from affected organs of foals on postmortem examination. the second aim was to demonstrate whether r. equi can be detected in smears of tracheal exudates collected from the same foals using an immunohistochemical method. impression smears and cryostat and paraffin-embedded sections were made from the lungs and mediastinal lymph nodes of three foals (a, b ... | 2001 | 11846020 |
| optimizing bioconversion pathways through systems analysis and metabolic engineering. | we demonstrate a general approach for metabolic engineering of biocatalytic systems comprising the uses of a chemostat for strain improvement and radioisotopic tracers for the quantification of pathway fluxes. flux determination allows the identification of target pathways for modification as validated by subsequent overexpression of the corresponding gene. we demonstrate this method in the indene bioconversion network of rhodococcus modified for the overproduction of 1,2-indandiol, a key precur ... | 2002 | 11854482 |
| in vitro evaluation of azd2563, a new oxazolidinone, tested against unusual gram-positive species. | the recently introduced oxazolidinone, linezolid, has a spectrum and potency directed against gram-positive organisms, including antimicrobial-resistant isolates. the newest agent in this class, azd2563 was tested against uncommonly isolated gram-positive species to establish the breadth of its spectrum. by reference broth microdilution methods, 120 strains were tested (48 corynebacterium spp., 10 species; 27 listeria spp., 2 species; 11 micrococcus spp., 2 species; 23 bacillus spp., 3 species; ... | 2002 | 11858907 |
| rhodococcus equi infection in non-hiv-infected patients. two case reports and review. | objective: to review two recent cases in hiv-negative subjects in the light of literature reports (52 patients without hiv infection till 1994). methods: epidemiology (animal contacts, risk factors, year, country), clinical presentation, diagnostic methods (x-ray, tomography, microbiological techniques), therapeutic approach (antibiotics, surgery) and outcome were evaluated on the basis of clinical literature reports. results: tumors constituted an important predisposing factor and less frequent ... | 1997 | 11864070 |
| secondary prophylaxis of rhodococcus equi pneumonia in hiv infection: breakthrough despite rifampicin/erythromycin suppressive therapy. | | 1997 | 11864093 |
| reactive oxygen species modulate zn(2+)-induced apoptosis in cancer cells. | some recent evidence has suggested a protective role of zinc against cancer. the mechanism by which zinc exerts this action has not been defined and, in particular, it has not been clarified whether zinc may directly act on cancer cells and the molecular mechanisms involved in this effect. in this study, we examined the in vitro effect of zinc on the apoptosis of mouse ts/a mammary adenocarcinoma cells, studying the zinc-dependent modulation of the intracellular levels of reactive oxygen species ... | 2002 | 11864783 |
| rhodococcus equi virulence-associated antigens and specific antibody response in aids patients infected with r. equi. | objectives: to analyze the expression of the 15- to 17-kda plasmid-encoded antigens from rhodococcus equi isolates of 7 aids patients and determine the immunologic response to these proteins in the patients' sera. methods: the expression of the virulence proteins in r. equi isolates and the specific antibody response were investigated by immunoblotting. plasmid dna was analyzed by agarose gel electrophoresis. results: the only patient infected with a strain carrying the virulence 85-kb plasmid a ... | 1995 | 11866716 |
| aseptic meningitis after neurosurgery: a demonstration of bacterial involvement. | objective: to evaluate the presence of bacteria in samples from patients suffering from 'aseptic' meningitis following craniotomy. methods: prospective study in which cerebrospinal fluid (csf) from patients suffering from post-craniotomy meningitis and negative control patients were submitted to conventional culture and to polymerase chain reaction (pcr) using bacterial 16s rrna universal primers, followed in some cases by dna sequencing of the pcr product and phylogenetic analysis. results: csf ... | 1996 | 11866771 |
| formaldehyde removal in synthetic and industrial wastewater by rhodococcus erythropolis upv-1. | rhodococcus erythropolis strain upv-1 is able to grow on phenol as the only carbon and energy source and to remove formaldehyde completely from both synthetic and industrial wastewater. the rate of formaldehyde removal is independent of either initial biomass or formaldehyde concentration. the presence of viable, intact cells is strictly necessary for this removal to take place. discontinuous and continuous formaldehyde-feed systems were successfully tested with synthetic wastewater in shaken fl ... | 2002 | 11876421 |
| [soft-tissue abscess caused by rhodococcus equi in a patient on hemodialysis]. | | 2001 | 11881436 |
| isocitrate lyase of the facultative intracellular pathogen rhodococcus equi. | isocitrate lyase is the first enzyme of the glyoxylate shunt which is required for the assimilation of fatty acids and acetate. the intracellular pathogen rhodococcus equi contains high activities of this enzyme following growth on acetate and lactate, indicating that it plays an important role in the metabolism of these substrates. the gene encoding isocitrate lyase (acea) was cloned and sequenced. it specifies a 46846 da protein, which was shown to be functional by expressing it in escherichia ... | 2002 | 11882714 |
| npd gene functions of rhodococcus (opacus) erythropolis hl pm-1 in the initial steps of 2,4,6-trinitrophenol degradation. | rhodococcus (opacus) erythropolis hl pm-1 grows on 2,4,6-trinitrophenol (picric acid) or 2,4-dinitrophenol (2,4-dnp) as sole nitrogen source. a gene cluster involved in picric acid degradation was recently identified. the functional assignment of three of its genes, npdc, npdg and npdi, and the tentative functional assignment of a fourth one, npdh, is reported. the genes were expressed in escherichia coli as his-tag fusion proteins that were purified by ni-affinity chromatography. the enzyme act ... | 2002 | 11882715 |
| peripheral benzodiazepine receptor ligands reverse apoptosis resistance of cancer cells in vitro and in vivo. | the mitochondrial peripheral benzodiazepine receptor (mpbr) is involved in a functional structure designated as the permeability transition pore, which controls apoptosis. binding of fas/apo-1/cd95 triggers a prototypic apoptosis-inducing pathway. using four different human tumor cell lines (t-cell jurkat, neuroblastoma shep, osteosarcoma 143n2, and glioblastoma snb79 cell lines), all of which express cd95 and mpbr, we investigated the potential role of mpbr ligands in cd95-induced apoptosis. we ... | 2002 | 11888910 |
| a novel streptomyces gene, samr, with different effects on differentiation of streptomyces ansochromogenes and streptomyces coelicolor. | a 1.4-kb dna fragment from streptomyces ansochromogenes accelerated mycelium formation of s. ansochromogenes when present on a multicopy plasmid. the dna fragment contains one complete open reading frame, designated samr, encoding a protein with 213 amino acids that contains a likely dna-binding helix-turn-helix motif close to its n-terminus. the deduced samr protein resembles the product of the hppr gene, which is involved in the regulation of catabolism of 3-(3-hydroxyphenyl) propionate in rho ... | 2002 | 11907684 |
| [the application of genetic engineering to the petroleum biodesulfurization]. | the developed course and reaction mechanisms of petroleum biodesulfurization were introduced. the recent development of genetic engineering technology, which used in desulfuration strain's construction, reconstruction and other fields, was summarized emphatically. its current research situation internal and overseas and the developing prospect were simply analyzed, and our research designs were submitted. | 2001 | 11910751 |
| [phylogenetic diversity of aerobic saprotrophic bacteria isolated from the daqing oil field]. | a diverse and active microbial community in the stratal waters of the daqing oil field (china), which is exploited with the use of water-flooding, was found to contain aerobic chemoheterotrophic bacteria (including hydrocarbon-oxidizing ones) and anaerobic fermentative, sulfate-reducing, and methanogenic bacteria. the aerobic bacteria were most abundant in the near-bottom zones of injection wells. twenty pure cultures of aerobic saprotrophic bacteria were isolated from the stratal waters. under ... | 2002 | 11910798 |
| rhodococcus erythropolis atcc 25544 as a suitable source of cholesterol oxidase: cell-linked and extracellular enzyme synthesis, purification and concentration. | the suitability of the strain rhodococcus erythropolis atcc 25544 grown in a two-liter fermentor as a source of cholesterol oxidase has been investigated. the strain produces both cell-linked and extracellular cholesterol oxidase in a high amount, that can be extracted, purified and concentrated by using the detergent triton x-114. | 2002 | 11914155 |
| pyogranulomatous skin disease and cellulitis in a cat caused by rhodococcus equi. | this report describes a case of rhodococcus equi infection causing pyogranulomatous skin disease and cellulitis in a two-year-old female domestic shorthaired cat. the case differed from previously reported cases in cats in its clinical presentation and in the locations of the lesions, which were similar to those seen in horses. the presence of an intracellular organism was confirmed by cytology and on histopathology. the aetiological diagnosis was confirmed by routine biochemical tests specific ... | 2002 | 11916057 |
| growth and production of cholesterol oxidase by alginate-immobilized cells of rhodococcus equi no. 23. | rhodococcus equi no. 23 was immobilized in calcium alginate. no detrimental effect on the viability of the test organism was observed during the immobilization procedure. approx. 98% of the cell population originally present in the alginate solution were immobilized in the gel beads. when the cells of an equal volume of the culture, obtained respectively at exponential phase (12 h preculture), late-exponential phase (20 h preculture) or stationary phase (36 h preculture) were immobilized, the ge ... | 2002 | 11916448 |
| rhodococcus jostii sp. nov., isolated from a medieval grave. | the taxonomic position of a bacterial strain isolated from the femur of the remains of jost lucemburský, margrave in moravia, brno (czech republic), was investigated by phenotypic, chemotaxonomic and molecular taxonomic methods. the chemotaxonomic characteristics, including the cell-wall amino acid and sugar compositions, the quinone system and the fatty acid profile, were in good agreement with those of the genus rhodococcus. the g+c content of the dna was 67.4 mol%. comparative 16s rrna gene s ... | 2002 | 11931149 |
| [role of cytology in the diagnosis of rhodococcus equi infection]. | a 59-year-old hiv-positive man developed necrotizing right-lobe pneumonia with abscess formation. bronchial aspirates and bronchoalveolar lavage showed numerous inflammatory cells, mainly histiocytes and neutrophils, and intracellular and extracellular gram- and ziehl-nielsen-positive cocci and coccobacilli. rhodococcus equi was identified. the patient died one week after diagnosis and necropsy confirmed the presence of r. equi nectrotizing pneumonia. | 2002 | 11938003 |
| placentitis, fetal pneumonia, and abortion due to rhodococcus equi infection in a thoroughbred. | rhodococcus equi is a rare cause of equine abortion. this report describes pyogranulomatous placentitis and fetal pneumonia in a case of abortion from a thoroughbred mare. numerous gram-positive coccobacilli were noted histologically within macrophages in placental and pulmonary lesions. rhodococcus equi was isolated in pure culture from the placenta, lung, liver, kidney, and stomach content. this is the first description of placentitis due to rhodococcus equi infection in a horse. | 2002 | 11939338 |
| [bacterial succession on n-alkanes under the conditions of sulfate reduction]. | the dynamics of species composition of a hydrocarbon-oxidizing bacteriocenosis of a ground suspension of mozhaisk reservoir has been studied. the bacteriocenosis was undergoing development in a paraffin film (model association composed of sulfate-reducing bacteria and hydrocarbon-oxidizing bacteria). the type of bacterial succession did not depend on the depth, from which ground samples were collected. two microbial species (pseudomonas sp. and arthrobacter globiformis) were absolutely dominant. ... | 2002 | 11962208 |
| dissemination of catabolic plasmids among desiccation-tolerant bacteria in soil microcosms. | the dissemination of catabolic plasmids was compared to bioaugmentation by strain inoculation in microcosm experiments. when rhodococcus erythropolis strain t902, bearing a plasmid with trichloroethene and isopropylbenzene degradation pathways, was used as the inoculum, no transconjugant was isolated but the strain remained in the soil. this plasmid had a narrow host range. pseudomonas putida strain c8s3 was used as the inoculum in a second approach. it bore a broad host range conjugative plasmi ... | 2001 | 11963851 |
| researchers wrestle with spread and control of emerging infections. | | 2002 | 11966362 |
| development of symbionts in triatomine bugs and the effects of infections with trypanosomatids. | in the intestinal tract of fifth instars of the hematophagous reduviid bugs rhodnius prolixus and triatoma infestans blood ingestion induced an initial decrease of the concentration of the respective symbiotic bacteria rhodococcus rhodnii and nocardia sp. and then within 10 days a 15- or 18-fold increase of the total population/bug to about 0.8 x 10(9) colony-forming units in r. prolixus and 1.8 x 10(9) colony-forming units in t. infestans. about 95-99% of the total populations of both symbionts ... | 2002 | 11971650 |
| extracellular polysaccharides of rhodococcus rhodochrous s-2 stimulate the degradation of aromatic components in crude oil by indigenous marine bacteria. | rhodococcus rhodochrous s-2 produces extracellular polysaccharides (s-2 eps) containing d-glucose, d-galactose, d-mannose, d-glucuronic acid, and lipids, which is important to the tolerance of this strain to an aromatic fraction of (af) arabian light crude oil (n. iwabuchi, n. sunairi, h. anzai, m. nakajima, and s. harayama, appl. environ. microbiol. 66:5073-5077, 2000). in the present study, we examined the effects of s-2 eps on the growth of indigenous marine bacteria on af. indigenous bacteri ... | 2002 | 11976106 |
| rhodococcus equi: an emerging pathogen. | more than 100 cases of rhodococcus equi infection have been reported since the first description of human disease caused by this organism. the vast majority of patients infected with r. equi are immunocompromised, and two-thirds have human immunodeficiency virus infection. the clinical manifestations of r. equi infection are diverse, although 80% of patients have some pulmonary involvement. the organism is easily cultured from specimens of infected tissue or body fluid, but it may be misdiagnose ... | 2002 | 11981734 |
| cell surface hydrophobicity and mycolic acid composition of rhodococcus strains isolated from activated sludge foam. | the bacteria causing foaming in activated sludge plants are considered to be hydrophobic, and their hydrophobicity is assumed to be a crucial factor in their foam-forming ability. this study showed no consistent relationship between cell surface hydrophobicity (csh), as determined by microbial adherence to hydrocarbons, of three rhodococcus spp. isolated from activated sludge foam and their ability to produce a stable foam. there also appeared to be no correlation between the mycolic acid compos ... | 2002 | 11986930 |
| identification of phenyldecanoic acid as a constituent of triacylglycerols and wax ester produced by rhodococcus opacus pd630. | phenyldecane supported growth and lipid accumulation of rhodococcus opacus pd630 during cultivation under nitrogen-limiting conditions. the results of this study suggested that the hydrocarbon phenyldecane was degraded by monoterminal oxidation, followed by beta-oxidation of the alkyl side-chain to phenylacetic acid, and by an additional degradative route for the oxidation of the latter to intermediates of the central metabolism. alpha-oxidation of phenyldecanoic acid also occurred to some exten ... | 2002 | 11988514 |
| intraspecific diversity of brevibacterium linens, corynebacterium glutamicum and rhodococcus erythropolis based on partial 16s rdna sequence analysis and fourier-transform infrared (ft-ir) spectroscopy. | the intraspecific diversity of 31 strains of brevibacterium linens, 27 strains of corynebacterium glutamicum and 29 strains of rhodococcus erythropolis was determined by partial 16s rdna sequence analysis and fourier-transform infrared (ft-ir) spectroscopy. as a prerequisite for the analyses, 27 strains derived from culture collections which had carried invalid or wrong species designations were reclassified in accordance with polyphasic taxonomical data. ft-ir spectroscopy proved to be a rapid ... | 2002 | 11988527 |
| formation and resuscitation of "non-culturable" cells of rhodococcus rhodochrous and mycobacterium tuberculosis in prolonged stationary phase. | after growth of rhodococcus rhodochrous in sauton's medium, and further incubation for about 60 h in stationary phase, there was a transient (up to 5 log) decrease in the c.f.u. count, whereas the total count remained similar to its initial value. at the point of minimal viability, the most probable number (mpn) count was 10 times greater than the c.f.u. count. this difference was further magnified by 3-4 logs (giving values close to the total count) by incorporating supernatant taken from growi ... | 2002 | 11988533 |
| isobutylidenediurea degradation by rhodococcus erythropolis. | a new enzyme (isobutylidenediurea amidinohydrolase) catalyzing the hydrolysis of isobutylidenediurea (a condensation product of urea and isobutyraldehyde widely used as a slow-release nitrogeneous fertilizer) was characterized from a strain of rhodococcus erythropolis. the enzyme was purified 1,250-fold to apparent homogeneity and shown to hydrolyze the fertilizer to urea and isobutyraldehyde at a molar ratio of 2: 1. no activity was observed with ureido- or other structurally related compounds. ... | 2001 | 11995825 |
| pharmacokinetics of azithromycin in foals after i.v. and oral dose and disposition into phagocytes. | the properties of azithromycin suggest that it may be an alternative to erythromycin for treatment of rhodococcus equi pneumonia in foals. to investigate this possibility, the disposition of azithromycin in plasma, polymorphonuclear leukocytes (pmn), and alveolar cells was examined after a single administration in foals. azithromycin suspension was administered orally (p.o.) at a dose of 10 mg/kg to five healthy 2-3-month-old foals. two weeks later, azithromycin for injection was administered by ... | 2002 | 12000529 |
| reductive deamination as a new step in the anaerobic microbial degradation of halogenated anilines. | in this paper we report the isolation and characterization of an anaerobic enrichment culture as well as of a rhodococcus sp. strain 2 capable of degrading 3,4-dihaloanilines under nitrate reducing conditions. using mass spectrometry several of the intermediates formed in the process of 3,4-dichloroaniline conversion were identified. most interesting is the observation of reductive deamination and the formation of 1,2-dichlorobenzene as one of the intermediates. using 19f nmr and fluorinated 3,4 ... | 2002 | 12007823 |
| survival and naphthalene-degrading activity of rhodococcus sp. strain 1bn in soil microcosms. | the survival and activity of rhodococcus sp. strain 1bn, inoculated into naphthalene-contaminated sandy-loam soil microcosms, were studied using classical and molecular methods. | 2002 | 12010546 |
| phages and other mobile virulence elements in gram-positive pathogens. | | 2002 | 12012871 |
| biodegradation of phenol in synthetic and industrial wastewater by rhodococcus erythropolis upv-1 immobilized in an air-stirred reactor with clarifier. | phenol biodegradation by suspended and immobilized cells of rhodococcus erythropolis upv-1 was studied in discontinuous and continuous mode under optimum culture conditions. phenol-acclimated cells were adsorbed on diatomaceous earth, where they grew actively forming a biofilm of short filaments. immobilization protected cells against phenol and resulted in a remarkable enhancement of their respiratory activity and a shorter lag phase preceding active phenol degradation. under optimum operation ... | 2002 | 12021809 |
| water transformation in the media of biofilters controlled by rhodococcus fascians in treating an ethyl acetate-contaminated airstream. | biofilters do not provide much water for bacteria to grow. to use them efficiently and properly, it is essential to understand the kinetics of water transformation and to control moisture levels. this study aims to clarify whether the metabolism of microorganisms will improve the water-holding capacity of media or will intensify drying. this experiment was conducted in duplicate, that is, both with and without bacterial inoculation. both the constant water content mode and the declining water co ... | 2002 | 12022691 |
| [antagonistic interactions between stress factors during the growth of microorganisms under conditions simulating the parameters of their natural ecotopes]. | two stress factors, hypoxia (microaerobic conditions) and a high salt concentration, if applied simultaneously to aerobic microorganisms, display an antagonistic mode of interaction. as a result, the nacl level that is usually optimal for moderate halophiles (5-6%) becomes optimal for the growth of weak halophiles (rhodococcus erythropolis and shewanella sp. cn32); the halotolerant yeast yarrowia lypolytica acquires halophilic properties (with a growth optimum at a nacl concentration of 10%), an ... | 2002 | 12024812 |
| [the role of low-molecular-weight nitrogen compounds in the osmotolerance of rhodococcus erythropolis and arthrobacter globiformis]. | investigations showed that rhodococcus erythropolis e-15 and arthrobacter globiformis 2f cells respond to osmotic shock by increasing the synthesis of free amino acids, primarily glutamic acid (80% of the intracellular free amino acid pool). the osmoprotective role of glutamic acid follows from its beneficial effect on the growth of bacteria in high-salinity media. it was found that the addition of this amino acid to the growth medium at a concentration of 2 mm shortened the lag phase and increa ... | 2002 | 12024813 |
| virulence genes of the phytopathogen rhodococcus fascians show specific spatial and temporal expression patterns during plant infection. | the phytopathogenic bacterium rhodococcus fascians provokes shoot meristem formation and malformations on aerial plant parts, mainly at the axils. the interaction is accompanied by bacterial colonization of the plant surface and tissues. upon infection, the two bacterial loci required for full virulence, fas and att, were expressed only at the sites of symptom development, although their expression profiles differed both spatially and temporally. the att locus was expressed principally in bacter ... | 2002 | 12026179 |
| hydrophobicity development, alkane oxidation, and crude-oil emulsification in a rhodococcus species. | the relationship between the phenomena alkane oxidation, extreme hydrophobicity of the cell surface, and crude-oil emulsification in rhodococcus sp. strain 094 was investigated. compounds that induce the emulsifying ability simultaneously induced the cytochrome p450-containing alkane oxidizing system and the transition from low to high cell-surface hydrophobicity. exposed to inducers of crude-oil emulsification, the cells developed a strong hydrophobic character during exponential growth, which ... | 2002 | 12030701 |
| discrimination and taxonomy of geographically diverse strains of nitrile-metabolizing actinomycetes using chemometric and molecular sequencing techniques. | mycolic acid-containing actinomycetes capable of metabolizing nitriles were recovered from deep-sea sediments and terrestrial soils by enrichment culture on acetonitrile, benzonitrile, succinonitrile or bromoxynil. a total of 43 nitrile-degrading strains were isolated and, together with previously recovered nitrile-degrading rhodococci, were identified by a polyphasic taxonomic approach, which included mycolic acid profiles, pyrolysis mass spectrometry (pyms), genomic fingerprinting based on seq ... | 2002 | 12030852 |
| cavitary lung lesions due to coinfection of rhodococcus equi and mycobacterium kansasii in a patient with acquired immunodeficiency syndrome. | | 2002 | 12034423 |
| 4-chlorocatechol 1,2-dioxygenase from the chlorophenol-utilizing gram-positive rhodococcus opacus 1cp: crystallization and preliminary crystallographic analysis. | 4-chlorocatechol 1,2-dioxygenase (4-clc1,2do) from the gram-positive bacterium rhodococcus opacus (erythropolis) 1cp, an enzyme involved in the aerobic biodegradation of chloroaromatic compounds, has been crystallized. 4-clc1,2do, which specifically catalyzes the intradiol cleavage of 4-substituted catechols, which are intermediates in the degradation of a variety of aromatic pollutants, to the corresponding maleylacetates, has recently been purified to homogeneity. the enzyme is an homodimer co ... | 2002 | 12037322 |
| degradation of phenol by rhodococcus erythropolis upv-1 immobilized on biolite in a packed-bed reactor. | a strain of rhodococcus erythropolis has been isolated and identified by 16s rrna sequencing. cells acclimated to phenol can be adsorbed on the external surface of beads of the ceramic support biolite where they grow forming a network of large filaments. exponentially-growing cells were adsorbed faster than their stationary-phase counterparts. immobilization resulted in a remarkable enhancement of the respiratory activity of cells and a shorter lag phase preceding the active phenol degradation. ... | 2002 | 12052678 |
| transfer of tsukamurella wratislaviensis goodfellow et a. 1995 to the genus rhodococcus as rhodococcus wratislaviensis comb. nov.. | a polyphasic study was undertaken to clarify the taxonomic position of the type strain (n805t) of tsukamurella wratislaviensis. this organism showed a combination of phenotypic properties, notably chemotaxonomic markers, consistent with its classification in the genus rhodococcus. comparative 16s rdna sequencing studies indicated that strain 805t falls into the rhodococcus erythropolis subclade, where it forms a monophyletic group with the type strains of rhodococcus opacus and rhodococcus perco ... | 2002 | 12054234 |
| substrate specificity of nickel/cobalt permeases: insights from mutants altered in transmembrane domains i and ii. | hoxn, a high-affinity, nickel-specific permease of ralstonia eutropha h16, and nhlf, a nickel/cobalt permease of rhodococcus rhodochrous j1, are structurally related members of the nickel/cobalt transporter (nicot) family. these transporters have an eight-helix structure and are characterized by highly conserved segments with polar or charged amino acid residues in transmembrane domains (tmds) ii, iii, v, and vi. two histidine residues in a ni2+ binding motif, the signature sequence of nicots, i ... | 2002 | 12057951 |
| rhodococcus equi brain abscess in an immunocompetent patient. | rhodococcus equi brain abscesses usually occur in immunocompromised patients with prolonged and refractory pulmonary infections. herein we report a case of r. equi brain abscess in a 67-y-old man without immunodepression. our patient recovered after neurosurgical resection and prolonged antimicrobial therapy with vancomycin and trimethoprim-sulfamethoxazole. | 2002 | 12064695 |
| h(2)o(2), which causes macrophage-related stress, triggers induction of expression of virulence-associated plasmid determinants in rhodococcus equi. | the response of the intracellular pathogen rhodococcus equi to h(2)o(2) treatment, a situation potentially encountered after the oxidative burst of alveolar macrophages, was analyzed. compared to other bacteria, including deinococcus radiodurans, r. equi showed exceptionally high resistance to this stress. a proteomic approach showed that four polypeptides present in the wild-type strain (85f) are missing in the plasmid-cured strain 85f(p-), and by using a dna macroarray, we identified two plasm ... | 2002 | 12065520 |
| population analysis of a binary bacterial culture by multi-parametric flow cytometry. | to study the degradation of a xenobiotic that requires a mixed culture it is essential to monitor the proportions and to control the population dynamics of the component strains. for these purposes fluorochromising techniques and multi-parametric flow cytometry were used to follow rhodococcus erythropolis k2-3 and ochrobactrum anthropi k2-14, both of which are needed to degrade 4-(2,4-dichlorophenoxy)butyric acid (2,4-db). although the two strains can grow in constant proportions in mixed cultur ... | 2002 | 12067522 |
| a novel lipoarabinomannan from the equine pathogen rhodococcus equi. structure and effect on macrophage cytokine production. | rhodococcus equi is a major cause of foal morbidity and mortality. we have investigated the presence of lipoglycan in this organism as closely related bacteria, notably mycobacterium tuberculosis, produce lipoarabinomannans (lam) that may play multiple roles as virulence determinants. the lipoglycan was structurally characterized by gas chromatography-mass spectrometry following permethylation, capillary electrophoresis after chemical degradation, and (1)h and (31)p and two-dimensional heteronuc ... | 2002 | 12072437 |
| cometabolic ring fission of dibenzofuran by gram-negative and gram-positive biphenyl-utilizing bacteria. | thirty-five strains of soil bacteria were grown with biphenyl (bp) and tested for their capacity to cooxidize dibenzofuran (dbf). during metabolism of dbf, the culture medium of 17 strains changed from colorless to orange, indicating a meta-cleavage pathway of dbf degradation. the ring cleavage product of these isolates was shown to be 2-hydroxy-4-(3'-oxo-3' h-benzofuran-2'-yliden)but-2-enoic acid (hobb). the strain sbug 271, studied in detail and identified as rhodococcus erythropolis, degraded ... | 2002 | 12073133 |
| selection of microbial consortia for treating metal-working fluids. | the aim of this research was to determine the effectiveness of a strategy for constructing microbial consortia for treating chemically mixed industrial effluent, based on a more thorough understanding of communities within waste metal-working fluids (mwfs). complementary phenotypic and genotypic methods revealed that the microbial communities in spent mwfs had low diversity and were very similar in species composition in samples originating from different locations and uses. of 65 bacterial isol ... | 2002 | 12080423 |
| family shuffling of a targeted bpha region to engineer biphenyl dioxygenase. | in this work we used a new strategy designed to reduce the size of the library that needs to be explored in family shuffling to evolve new biphenyl dioxygenases (bpdos). instead of shuffling the whole gene, we have targeted a fragment of bpha that is critical for enzyme specificity. we also describe a new protocol to screen for more potent bpdos that is based on the detection of catechol metabolites from chlorobiphenyls. several bpha variants with extended potency to degrade polychlorinated biph ... | 2002 | 12081948 |
| identification of a new class of cytochrome p450 from a rhodococcus sp. | a degenerate set of pcr primers were used to clone a gene encoding a cytochrome p450 (the p450rhf gene) from rhodococcus sp. strain ncimb 9784 which is of unique primary structural organization. surprisingly, analysis of the translation product revealed that the p450 is fused to a reductase domain at the c terminus which displays sequence conservation for dioxygenase reductase proteins. the reductase partner comprises flavin mononucleotide- and nadh-binding motifs and a [2fe2s] ferredoxin-like c ... | 2002 | 12081961 |
| monocyclic aromatic hydrocarbon degradation by rhodococcus sp. strain dk17. | rhodococcus sp. strain dk17 was isolated from soil and analyzed for the ability to grow on o-xylene as the sole carbon and energy source. although dk17 cannot grow on m- and p-xylene, it is capable of growth on benzene, phenol, toluene, ethylbenzene, isopropylbenzene, and other alkylbenzene isomers. one uv-generated mutant strain, dk176, simultaneously lost the ability to grow on o-xylene, ethylbenzene, isopropylbenzene, toluene, and benzene, although it could still grow on phenol. the mutant st ... | 2002 | 12089003 |
| cloning and heterologous expression of an enantioselective amidase from rhodococcus erythropolis strain mp50. | the gene for an enantioselective amidase was cloned from rhodococcus erythropolis mp50, which utilizes various aromatic nitriles via a nitrile hydratase/amidase system as nitrogen sources. the gene encoded a protein of 525 amino acids which corresponded to a protein with a molecular mass of 55.5 kda. the deduced complete amino acid sequence showed homology to other enantioselective amidases from different bacterial genera. the nucleotide sequence approximately 2.5 kb upstream and downstream of t ... | 2002 | 12089004 |
| biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene. | using a combined strategy of random mutagenesis of haloalkane dehalogenase and genetic engineering of a chloropropanol-utilizing bacterium, we constructed an organism that is capable of growth on 1,2,3-trichloropropane (tcp). this highly toxic and recalcitrant compound is a waste product generated from the manufacture of the industrial chemical epichlorohydrin. attempts to select and enrich bacterial cultures that can degrade tcp from environmental samples have repeatedly been unsuccessful, proh ... | 2002 | 12089046 |
| increased pulmonary activation of nuclear factor-kappab (nf-kappab) in foals inoculated with rhodococcus equi is associated with increased expression of inflammatory cytokines. | previous studies revealed that foals inoculated with virulent rhodococcus equi had significantly higher pulmonary levels of interleukin-1beta, interleukin-12 p40, interferon-gamma, and tumor necrosis factor-alpha mrna compared to foals inoculated with an avirulent plasmid-cured derivative. the purpose of this study was to determine if the increases in cytokine expression were associated with increased pulmonary activation of nuclear factor-kappab (nf-kappab). electrophoretic mobility shift assay ... | 2002 | 12102205 |
| [rhodococcus equi infection: a rare cause of cutaneous vasculitis]. | | 2002 | 12108184 |
| alkanotrophic rhodococcus ruber as a biosurfactant producer. | in this report we examined the structure and properties of surface-active lipids of rhodococcus ruber. most historical interest has been in the glycolipids of rhodococcus erythropolis, which have been extensively characterised. r. erythropolis has been of interest due to its great metabolic diversity. only recently has the metabolic potential of r. ruber begun to be explored. one major difference in the two species is that most r. ruber strains are able to oxidise the gaseous alkanes propane and ... | 2002 | 12111164 |
| biodesulfurization of benzothiophene and dibenzothiophene by a newly isolated rhodococcus strain. | rhodococcus sp. kt462, which can grow on either benzothiophene (bt) or dibenzothiophene (dbt) as the sole source of sulfur, was newly isolated and characterized. gc and gc-ms analyses revealed that strain kt462 has the same bt desulfurization pathway as that reported for paenibacillus sp. a11-2 and sinorhizobium sp. kt55. the desulfurized product of dbt produced by this strain, as well as other dbt-desulfurizing bacteria such as r. erythropolis ka2-5-1 and r. erythropolis igts8, was 2-hydroxybip ... | 2002 | 12111165 |
| a two-step, one-pot enzymatic synthesis of cephalexin from d-phenylglycine nitrile. | a cascade of two enzymatic transformations is employed in a one-pot synthesis of cephalexin. the nitrile hydratase (from r. rhodochrous mawe)-catalyzed hydration of d-phenylglycine nitrile to the corresponding amide was combined with the penicillin g acylase (penicillin amidohydrolase, e.c. 3.5.1.11)-catalyzed acylation of 7-adca with the in situ-formed amide to afford a two-step, one-pot synthesis of cephalexin. d-phenylglycine nitrile appeared to have a remarkable selective inhibitory effect o ... | 2002 | 12115424 |
| site-directed mutagenesis for cysteine residues of cobalt-containing nitrile hydratase. | three cysteine residues, which are completely conserved among alpha-subunits in all nitrile hydratases, are thought to be the ligands of a metal ion in the catalytic center of this enzyme. these cysteine residues (i.e. alpha c102, alpha c105 and alpha c107) in the high-molecular-mass nitrile hydratase (h-nhase) of rhodococcus rhodochrous j1 were replaced with alanine by site-directed mutagenesis using the r. rhodochrous atcc12674 host-vector system, and the resultant transformants were investiga ... | 2002 | 12121763 |
| rhodococcus equi pulmonary infection in a pancreas-alone transplant recipient: consequence of intense immunosuppression. | we report the case of a pancreas-alone transplant recipient who developed rhodococcus equi pneumonia after receiving multiple courses of antilymphocyte therapy for the treatment of recurrent acute pancreas allograft rejection. we also review and discuss the diagnosis, clinical course, and treatment of 18 cases of r. equi infection reported in solid organ transplant recipients. the lung is the most common primary site of infection, but r. equi infection is difficult to diagnose because of the ple ... | 2002 | 12123426 |
| rhodococcus equi infection in transplant recipients: a case of mistaken identity and review of the literature. | the incidence of rhodococcus equi infection in solid-organ transplant recipients continues to rise throughout the world. unfortunately, this opportunistic pathogen is still underestimated and potentially disregarded by physicians and microbiology laboratories due to its morphology on gram staining. pulmonary involvement is the most common finding in the immunocompromised host. we report a case of a 63-year-old heart-transplant recipient who presented with increasing fatigue and nonproductive cou ... | 2002 | 12123427 |
| rhodococcus equi: pulmonary cavitation lesion in patient infected with hiv cured by levofloxacin and rifampicin. | | 2002 | 12131231 |
| use of both 16s rrna and engineered functional genes with real-time pcr to quantify an engineered, pcb-degrading rhodococcus in soil. | a real-time pcr (rtm-pcr) assay using fluorescently labeled oligonucleotides (taqman probes) was used to detect and quantify the recombinant rhodococcus sp. strain rha1(fcb) in soil. one primer and probe set targeted a hypervariable region of the 16s rrna gene unique to strain rha1(fcb) and its phylogenetic relatives, and the other set targeted the recombinant 4-chlorobenzoate (4-cba) degradation operon (fcb) and was strain-specific. the method had a 6-log dynamic range of detection (10(2)-10(7) ... | 2002 | 12133610 |
| identification of virulence-associated antigens and plasmids in rhodococcus equi from patients with acquired immune deficiency syndrome and prevalence of virulent r. equi in soil collected from domestic animal farms in chiang mai, thailand. | the prevalence of virulent rhodococcus equi in soil collected from 17 domestic animal farms (from 12 cattle, 1 pig, and 4 horse farms) and in 6 clinical specimens from patients with acquired immune deficiency syndrome (aids) in chiang mai, thailand, was investigated. the isolates were tested for the presence of 15-17-kda antigens (vapa) and a 20-kda antigen (vapb) by immunoblotting and for the presence of virulence plasmid dna. rhodococcus equi was isolated from most soil samples (68 of 80) obta ... | 2002 | 12135268 |
| [the efficient accumulation of cesium ions by rhodococcus cells]. | bacteria of the genus rhodococcus were found to be able to accumulate cesium by means of active transport and nonspecific sorption on the cell surface structures. the maximum removal (up to 97%) of cesium from a medium with ammonium acetate was observed at 28 degrees c, ph 7.8-8.6, and an equimolar content (0.2 mm) of potassium and cesium ions in the medium. the most active cesium-accumulating rhodococcus sp. strains can be used for purification of industrial wastewaters contaminated with radion ... | 2002 | 12138767 |
| a novel enzyme, 2'-hydroxybiphenyl-2-sulfinate desulfinase (dszb), from a dibenzothiophene-desulfurizing bacterium rhodococcus erythropolis ka2-5-1: gene overexpression and enzyme characterization. | dibenzothiophene (dbt), a model of organic sulfur compound in petroleum, is microbially desulfurized to 2-hydroxybiphenyl (2-hbp), and the gene operon dszabc was required for dbt desulfurization. the final step in the microbial dbt desulfurization is the conversion of 2'-hydroxybiphenyl-2-sulfinate (hbpsi) to 2-hbp catalyzed by dszb. in this study, dszb of a dbt-desulfurizing bacterium rhodococcus erythropolis ka2-5-1 was overproduced in escherichia coli by coexpression with chaperonin genes, gr ... | 2002 | 12147352 |
| plasmid-borne genes code for an angular dioxygenase involved in dibenzofuran degradation by terrabacter sp. strain yk3. | the genes responsible for angular dioxygenation of dibenzofuran in actinomycetes were cloned by using a degenerate set of pcr primers designed by using conserved sequences of the dioxygenase alpha subunit genes. one sequence of alpha subunit genes was commonly amplified from four dibenzofuran-utilizing actinomycetes: terrabacter sp. strains yk1 and yk3, rhodococcus sp. strain yk2, and microbacterium sp. strain yk18. a 5.2-kb psti fragment encoding the alpha and beta subunits of the terminal diox ... | 2002 | 12147464 |
| biodesulfurization of naphthothiophene and benzothiophene through selective cleavage of carbon-sulfur bonds by rhodococcus sp. strain wu-k2r. | naphtho[2,1-b]thiophene (nth) is an asymmetric structural isomer of dibenzothiophene (dbt), and in addition to dbt derivatives, nth derivatives can also be detected in diesel oil following hydrodesulfurization treatment. rhodococcus sp. strain wu-k2r was newly isolated from soil for its ability to grow in a medium with nth as the sole source of sulfur, and growing cells of wu-k2r degraded 0.27 mm nth within 7 days. wu-k2r could also grow in the medium with nth sulfone, benzothiophene (bth), 3-me ... | 2002 | 12147483 |
| [study on production of acrylamide by microbial method (ii)--enzyme catalytic kinetics and de-active dynamics of nitrile hydratase]. | the hydration reaction by microbial method is the crisis of the procedure of acrylamide production from acrylonitrile. this research studied the enzyme catalytic kinetics and de-active kinetics of nitrile hydratase in the type of free cell. firstly, the effects of the concentration of cells, the temperature, ph value, the concentration of acrylonitrile and the concentration of acrylamide on the activity of nitrile hydratase was investigated. the result is that the temperature and the concentrati ... | 2002 | 12148289 |
| detection of rhodococcus equi by polymerase chain reaction using species-specific nonproprietary primers. | species-specific primers for the polymerase chain reaction (pcr) for the detection of rhodococcus equi were developed. these primers were based on unique dna fragments produced from r. equi reference strains and field isolates. following random amplification of polymorphic dna from r. equi and r. rhodochrous with a set of 40 arbitrary 10-base pair (bp) primers, a pair of species-specific primers was designed to detect a unique 700-bp fragment of r. equi chromosomal dna. this pcr product was limi ... | 2002 | 12152820 |
| tolerance of bacteria to organic solvents. | organic-solvent-tolerant bacteria are a relatively novel group of extremophilic microorganisms. they overcome the toxic and destructive effects of organic solvents due to the presence of various adaptive mechanisms. extensive studies done on the toluene tolerance of certain pseudomonas strains have led to an understanding of the mechanisms of organic solvent tolerance involving novel adaptations such as the toluene efflux pumps, cis-trans isomerisation of membrane fatty acids, rapid membrane rep ... | 2002 | 12160316 |
| engineering an indene bioconversion process for the production of cis-aminoindanol: a model system for the production of chiral synthons. | cis-aminoindanol, a key chiral precursor to the hiv protease inhibitor crixivan, can be derived from indene oxidation products of (2r) stereochemistry. a number of different microorganisms, notably strains of the genera pseudomonas and rhodococcus, have been isolated that catalyze the oxygenation of indene to indandiol with greater stereospecificity than is achievable through traditional chemical synthesis. the yield and ultimate optical purity of indandiol produced in such biocatalytic processe ... | 2002 | 12172601 |
| p450(camr), a cytochrome p450 catalysing the stereospecific 6- endo-hydroxylation of (1 r)-(+)-camphor. | rhodococcus sp. ncimb 9784 accumulated 6- endo-hydroxycamphor 3 when grown on (1 r)-(+)-camphor 1 as sole carbon source. the structure of 3 has been unambiguously assigned for the first time using x-ray crystallography. a soluble cytochrome p450 hydroxylase, induced by growth on (1 r)-(+)-camphor and designated p450(camr), has been isolated from the bacterium rhodococcus sp. ncimb 9784. using authentic 6- endo hydroxycamphor as standard, a cell-free system consisting of pure p450(camr) and putid ... | 2002 | 12172608 |
| population dynamics of free-floating and attached bacteria in a styrene-degrading biotrickling filter analyzed by denaturing gradient gel electrophoresis. | population dynamics was studied in a 52-l biotrickling filter (btf) operated for 182 days and used to clean air contaminated with styrene vapors. in the btf, biomass grew either as free-floating (planktonic) or attached (sessile) microorganisms. pcr-amplified 16s rdna fragments from planktonic and sessile cells within the bioreactor were analyzed using denaturing gradient gel electrophoresis (dgge). the results indicated that the complexity of biofilm community was always more pronounced than th ... | 2002 | 12172630 |
| evidence that rdx biodegradation by rhodococcus strain dn22 is plasmid-borne and involves a cytochrome p-450. | to investigate the biodegradation of the explosive compound rdx in rhodococcus strain dn22, a bacterium previously isolated for its ability to grow on rdx as sole nitrogen source. | 2002 | 12174045 |
| the principal determinants for the structure of the substrate-binding pocket are located within a central core of a biphenyl dioxygenase alpha subunit. | protein engineering by segment exchange was used to distinguish between regions of major and minor influence on the structure of the substrate-binding pocket of a biphenyl dioxygenase (bdo). eight chimaeric enzyme systems were generated that each consisted of a hybrid hydroxylase alpha subunit (bpha1) containing segments from burkholderia sp. strain lb400 and rhodococcus globerulus p6, and of a hydroxylase beta subunit (bpha2), a ferredoxin (bpha3) and a ferredoxin reductase (bpha4) from strain ... | 2002 | 12177337 |
| molecular and functional characterization of ksha and kshb, encoding two components of 3-ketosteroid 9alpha-hydroxylase, a class ia monooxygenase, in rhodococcus erythropolis strain sq1. | 9 alpha-hydroxylation of 4-androstene-3,17-dione (ad) and 1,4-androstadiene-3,17-dione (add) is catalysed by 3-ketosteroid 9 alpha-hydroxylase (ksh), a key enzyme in microbial steroid catabolism. very limited knowledge is presently available on the ksh enzyme. here, we report for the first time the identification and molecular characterization of genes encoding ksh activity. the ksha and kshb genes, encoding ksh in rhodococcus erythropolis strain sq1, were cloned by functional complementation of ... | 2002 | 12180920 |
| crystal structures of a meta-cleavage product hydrolase from pseudomonas fluorescens ip01 (cumd) complexed with cleavage products. | 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase (cumd) from pseudomonas fluorescens ip01 hydrolyzes a meta-cleavage product generated in the cumene (isopropylbenzene) degradation pathway. the crystal structures of the inactive s103a mutant of the cumd enzyme complexed with isobutyrate and acetate ions were determined at 1.6 and 2.0 a resolution, respectively. the isobutyrate and acetate ions were located at the same position in the active site, and occupied the site for a part of the hydroly ... | 2002 | 12192074 |
| recombinant rhodococcus sp. strain t09 can desulfurize dbt in the presence of inorganic sulfate. | the putative rhodococcus rrn promoter region was cloned from the benzothiophene desulfurizing rhodococcus sp. strain t09, and the dibenzothiophene desulfurizing gene, dsz, was expressed under the control of the putative rrn promoter in the strain t09 using a rhodococcus-e.coli shuttle vector. strain t09 harboring the expression vector, pnt, could desulfurize dibenzothiophene in the presence of inorganic sulfate, methionine, or cysteine, while the dsz phenotype was completely repressed in recombi ... | 2002 | 12192519 |
| rhamnolipid stimulates uptake of hydrophobic compounds by pseudomonas aeruginosa. | the biodegradation of hexadecane by five biosurfactant-producing bacterial strains (pseudomonas aeruginosa ug2, acinetobacter calcoaceticus rag1, rhodococcus erythropolis dsm 43066, r. erythropolis atcc 19558, and strain bcg112) was determined in the presence and absence of exogenously added biosurfactants. the degradation of hexadecane by p. aeruginosa was stimulated only by the rhamnolipid biosurfactant produced by the same organism. this rhamnolipid did not stimulate the biodegradation of hex ... | 2002 | 12200306 |
| practical and convenient enzymatic synthesis of enantiopure alpha-amino acids and amides. | catalyzed by the nitrile hydratase and the amidease in rhodococcus sp. aj270 cells under very mild conditions, a number of alpha-aryl- and alpha-alkyl-substituted dl-glycine nitriles 1 rapidly underwent a highly enantioselective hydrolysis to afford d-(-)-alpha-amino acid amides 2 and l-(+)-alpha-amino acids 3 in high yields with excellent enantiomeric excesses in most cases. the overall enantioselectivity of the biotransformations of nitriles originated from the combined effects of a high l-ena ... | 2002 | 12201779 |
| fighting foam with phages? | seventeen (17) phages infective for the mycolata were isolated from six samples of activated sludge using 21 prospective hosts from the genera dietzia, gordonia, nocardia, rhodococcus, tsukamurella and mycobacterium. their morphology indicated that they were all members of the viral family siphoviridae, but they varied in the size of the icosahedral head and length of non-contractile tail, suggesting they were different. this was confirmed by host-range studies with 47 strains of mycolata, which ... | 2002 | 12216679 |