| intraspecies variability of desulfovibrio desulfuricans strains determined by the genetic profiles. | fifteen (soil and intestinal) strains of desulfovibrio desulfuricans species were typed by pcr method with the use of primers specific for repetitive extragenic palindromic (rep) and enterobacterial repetitive intergenic consensus (eric) sequences. as a result, characteristic dna fingerprints for the strains were obtained. moreover, the genetic profiles were found to be useful for typing and distinguishing the strains of d. desulfuricans. according to cluster analysis, pcr with primers complemen ... | 2003 | 12594025 |
| mechanism of flavin mononucleotide cofactor binding to the desulfovibrio vulgaris flavodoxin. 1. kinetic evidence for cooperative effects associated with the binding of inorganic phosphate and the 5'-phosphate moiety of the cofactor. | the pathway(s) by which the flavin cofactor binds to the apoflavoprotein is the subject of some debate. the crystal and nmr structures of several different flavodoxins have provided some insight, although there is disagreement about the location of the initial interaction between the flavin mononucleotide (fmn) and the apoflavodoxin and the degree of protein conformational change associated with cofactor binding [genzor, c. g., perales-alcon, a., sancho, j., and romero, a. (1996) nat. struct. bi ... | 2003 | 12600198 |
| mechanism of flavin mononucleotide cofactor binding to the desulfovibrio vulgaris flavodoxin. 2. evidence for cooperative conformational changes involving tryptophan 60 in the interaction between the phosphate- and ring-binding subsites. | a mechanism has been proposed for the binding of flavin mononucleotide (fmn) and riboflavin to the apoflavodoxin from desulfovibrio vulgaris [murray, t. a., and swenson, r. p. (2003) biochemistry 42, 2307-2316]. in this model, the binding of the flavin isoalloxazine ring is dependent on the presence of a phosphate moiety in the phosphate-binding subsite, suggesting a cooperative interaction between that region and the ring-binding subsite. in the absence of inorganic phosphate, fmn can bind thro ... | 2003 | 12600199 |
| biological activity of desulfovibrio desulfuricans lipopolysaccharides evaluated via interleukin-8 secretion by caco-2 cells. | although desulfovibrio desulfuricans species, besides existing in the natural environment, is also found in the human digestive tract, no information is currently available on its role in the intestinal ecosystem and its activity in regard to the intestinal mucosa. bacterial products (lipopolysaccharides, lpss) are generally known for their ability to trigger inflammatory response by stimulating cytokine expression, such as interleukin-8 (il-8). | 2003 | 12608468 |
| cytochrome c nitrite reductase from desulfovibrio desulfuricans atcc 27774. the relevance of the two calcium sites in the structure of the catalytic subunit (nrfa). | the gene encoding cytochrome c nitrite reductase (nrfa) from desulfovibrio desulfuricans atcc 27774 was sequenced and the crystal structure of the enzyme was determined to 2.3-a resolution. in comparison with homologous structures, it presents structural differences mainly located at the regions surrounding the putative substrate inlet and product outlet, and includes a well defined second calcium site with octahedral geometry, coordinated to propionates of hemes 3 and 4, and caged by a loop non ... | 2003 | 12618432 |
| activity of sulfate-reducing bacteria in human periodontal pocket. | samples of subgingival dental tissues were examined for the presence of sulfate-reducing bacteria (srb). using enrichment cultures, srbs were detected in 9 of 17 individuals. a pure culture of srb was obtained from one sample collected from a patient with type iv periodontal disease. the characterization of this isolate showed that it belongs to the genus desulfovibrio. the isolate used pyruvate, lactate, glucose, fructose, and ethanol as the sole source of carbon. however, the isolate was unabl ... | 2002 | 12619823 |
| isolation of sulfate-reducing bacteria from human thoracoabdominal pus. | to evaluate the prevalence of sulfate-reducing bacteria in septic processes, we searched for these bacteria by culture in 100 consecutive abdominal and pleural pus specimens. twelve isolates were obtained from abdominal samples and were identified by a multiplex pcr as desulfovibrio piger (formerly desulfomonas pigra) (seven strains), desulfovibrio fairfieldensis (four strains), and desulfovibrio desulfuricans (one strain). | 2003 | 12624073 |
| the nature of the di-iron site in the bacterioferritin from desulfovibrio desulfuricans. | the first crystal structure of a native di-iron center in an iron-storage protein (bacterio)ferritin is reported. the protein, isolated from the anaerobic bacterium desulfovibrio desulfuricans, has the unique property of having fe-coproporphyrin iii as its heme cofactor. the three-dimensional structure of this bacterioferritin was determined in three distinct catalytic/redox states by x-ray crystallography (at 1.95, 2.05 and 2.35 a resolution), corresponding to different intermediates of the di- ... | 2003 | 12627224 |
| an engineered two-iron superoxide reductase lacking the [fe(scys)4] site retains its catalytic properties in vitro and in vivo. | superoxide reductases (sors) contain a characteristic square-pyramidal [fe(nhis)(4)(scys)] active site that catalyzes reduction of superoxide to hydrogen peroxide in several anaerobic bacteria and archaea. some sors, referred to as two-iron sors (2fe-sors), also contain a lower-potential [fe(scys)(4)] site that is presumed to have an electron transfer function. however, the intra- and inter-subunit distances between [fe(scys)(4)] and [fe(nhis)(4)(scys)] iron centers within the 2fe-sor homodimer ... | 2003 | 12637682 |
| 1h, 13c and 15n assignment of the hydroquinone form of flavodoxin from desulfovibrio vulgaris (hildenborough) and comparison of the chemical shift differences with respect to the oxidized state. | | 2003 | 12652141 |
| the effect of 5-aminosalicylic acid-containing drugs on sulfide production by sulfate-reducing and amino acid-fermenting bacteria. | the toxic, bacterial metabolite sulfide is implicated in ulcerative colitis. ulcerative colitis patients taking 5-aminosalicylic acid-containing drugs have lower fecal sulfide levels than those not taking these drugs. the effects of sulfasalazine, balsalazide, olsalazine, and 5-aminosalicylic acid on sulfide production were studied in a three-stage chemostat pulsed on days 1 to 3 with 5 g sulfasalazine (40 mm) and in pure cultures of amino acid-fermenting and sulfate-reducing bacteria. by the th ... | 2003 | 12656132 |
| desulfovibrio hydrothermalis sp. nov., a novel sulfate-reducing bacterium isolated from hydrothermal vents. | mesophilic, hydrogenotrophic, sulfate-reducing bacteria were isolated from a deep-sea hydrothermal chimney sample collected at 13 degrees n on the east-pacific rise at a depth of 2,600 m. two strains (bl5 and h9) were found to be phylogenetically similar to desulfovibrio profundus (similarity >99%), whereas two other strains (h1 and am13t) were found to be phylogenetically distinct (similarity 96.4%) from desulfovibrio zosterae, their closest relative. strain am13t was characterized further. it ... | 2003 | 12656169 |
| structure of dimeric cytochrome c3 from desulfovibrio gigas at 1.2 a resolution. | the structure of dimeric cytochrome c(3) from the sulfate-reducing bacterium desulfovibrio gigas, didg, obtained by ab initio methods was further refined to 1.2 a resolution, giving final reliability factors of r(free) = 14.8% and r = 12.4%. this cytochrome is a dimer of tetraheme cytochrome c(3) molecules covalently linked by two solvent-accessible disulfide bridges, a characteristic unique to members of the cytochrome c(3) superfamily. anisotropic analysis using the semi-rigid tls method shows ... | 2003 | 12657783 |
| [effect of various anions on the rate of microbe-induced corrosion]. | experimental corroboration of correctness of theoretical thermodynamic calculations of e.m.f. of corrosion reactions induced by soil microorganisms is obtained in the work. a hypothesis is put forward on possible mechanism for stimulation of microbe-induced corrosion by chloride ions. the results obtained permit revealing the reasons of low efficiency conditions of cathode protection in cases of active involvement of soil microorganisms into corrosion processes which are used for maintenance of ... | 2002 | 12664552 |
| [effect of sulfate-reducing bacteria on steel corrosion in the presence of inhibitors]. | steel 08kp corrosion was studied as affected by inhibitors in presence of sulphate-reducing bacteria (srb). organic compounds, containing functional groups with nitrogen, oxygen and sulphur atoms, were investigated as corrosion inhibitors. it is shown that the studied inhibitors may be divided into three groups as to the mechanism of protective action. it has been established that cation-active nitrogen-containing surfactants ([symbol: see text] x, [symbol: see text]-1, [symbol: see text]-1m, ca ... | 2002 | 12664553 |
| mase1 and mase2: two novel integral membrane sensory domains. | escherichia coli proteins yege and yaic contain n-terminal integral membrane regions, followed by the putative diguanylate cyclase (ggdef, duf1) domains. the membrane domains of these proteins, named mase1 (membrane-associated sensor) and mase2, respectively, were found in other bacterial signaling proteins, such as histidine kinases (mase1) and an adenylate cyclase (mase2). although the nature of the signals sensed by mase1 and mase2 is still unknown, mase1-containing receptors appear to play i ... | 2003 | 12673057 |
| spatial and temporal analysis of the microbial community in slow sand filters used for treating horticultural irrigation water. | an experimental slow sand filter (ssf) was constructed to study the spatial and temporal structure of a bacterial community suppressive to an oomycete plant pathogen, phytophthora cryptogea. passage of water through the mature sand column resulted in complete removal of zoospores of the plant pathogen. to monitor global changes in the microbial community, bacterial and fungal numbers were estimated on selective media, direct viable counts of fungal spores were made, and the atp content was measu ... | 2003 | 12676691 |
| reduction of np(v) and precipitation of np(iv) by an anaerobic microbial consortium. | a combination of experimental, analytical, and modeling investigations shows that an anaerobic, sulfate-reducing consortium reduced np(v) to np(iv), with subsequent precipitation of a np(iv) solid. precipitation of np(iv) during growth on pyruvate occurred before sulfate reduction began. h2 stimulated precipitation of np(iv) when added alone to growing cells, but it slowed precipitation when added along with pyruvate. increasing concentrations of pyruvate also retarded precipitation. accumulatio ... | 2002 | 12688585 |
| a procedure for quantitation of total oxidized uranium for bioremediation studies. | a procedure was developed for the quantitation of complexed u(vi) during studies on u(vi) bioremediation. these studies typically involve conversion of soluble or complexed u(vi) (oxidized) to u(iv) (the reduced form which is much less soluble). since u(vi) freely exchanges between material adsorbed to the solid phase and the dissolved phase, uranium bioremediation experiments require a mass balance of u in both its soluble and adsorbed forms as well as in the reduced sediment bound phase. we se ... | 2003 | 12689712 |
| computational study of the non-heme iron active site in superoxide reductase and its reaction with superoxide. | the ferrous square-pyramidal [fe(nhis)4(scys)] site of superoxide reductases (sors) has been shown to reduce superoxide at a nearly diffusion-controlled rate. the final products of the reaction are hydrogen peroxide and the ferric hexacoordinated sor site, with a carboxylate group from a conserved glutamate serving as the sixth ligand trans to the cysteine sulfur. a transient intermediate absorbing at approximately 600 nm in the reaction of the ferrous pentacoordinated site with superoxide has b ... | 2003 | 12693226 |
| a novel electrobiotechnology for the recovery of precious metals from spent automotive catalysts. | platinum group metals are routinely used in automotive catalysts but recycle technology lags behind demand. there is no available 'dean technology' and leach solutions (e.g. aqua regia) to solubilise the metals from scrap are highly aggressive. a microwave-assisted leaching method was developed which gave 80% metals recovery, with the leach time reduced from 2 h to 15 min using 50% (aq.) diluted aqua regia to give potentially a more biocompatible leachate. desulfovibrio desulfuricans reduces sol ... | 2003 | 12703854 |
| stable carbon isotope fractionation by sulfate-reducing bacteria. | biogeochemical transformations occurring in the anoxic zones of stratified sedimentary microbial communities can profoundly influence the isotopic and organic signatures preserved in the fossil record. accordingly, we have determined carbon isotope discrimination that is associated with both heterotrophic and lithotrophic growth of pure cultures of sulfate-reducing bacteria (srb). for heterotrophic-growth experiments, substrate consumption was monitored to completion. sealed vessels containing s ... | 2003 | 12732570 |
| sequence versus structure for the direct detection of 16s rrna on planar oligonucleotide microarrays. | a two-probe proximal chaperone detection system consisting of a species-specific capture probe for the microarray and a labeled, proximal chaperone probe for detection was recently described for direct detection of intact rrnas from environmental samples on oligonucleotide arrays. in this study, we investigated the physical spacing and nucleotide mismatch tolerance between capture and proximal chaperone detector probes that are required to achieve species-specific 16s rrna detection for the diss ... | 2003 | 12732571 |
| redox-bohr and other cooperativity effects in the nine-heme cytochrome c from desulfovibrio desulfuricans atcc 27774: crystallographic and modeling studies. | the nine-heme cytochrome c is a monomeric multiheme cytochrome found in desulfovibrio desulfuricans atcc 27774. the polypeptide chain comprises 296 residues and wraps around nine hemes of type c. it is believed to take part in the periplasmic assembly of proteins involved in the mechanism of hydrogen cycling, receiving electrons from the tetraheme cytochrome c3. with the purpose of understanding the molecular basis of electron transfer processes in this cytochrome, we have determined the crystal ... | 2003 | 12750363 |
| copper resistance in desulfovibrio strain r2. | a sulfate-reducing bacterium, designated as strain r2, was isolated from wastewater of a ball-bearing manufacturing facility in tomsk, western siberia. this isolate was resistant up to 800 mg cu/l in the growth medium. by comparison, cu-resistance of reference cultures of sulfate-reducing bacteria ranged from 50 to 75 mg cu/l. growth experiments with strain r2 showed that cu was an essential trace element and, on one hand, enhanced growth at concentrations up to 10 mg/l but, on the other hand, t ... | 2003 | 12755486 |
| epr and endor evidence for a 1-his, hydroxo-bridged mixed-valent diiron site in desulfovibrio vulgaris rubrerythrin. | key features differentiating the coordination environment of the two irons in the mixed-valent (fe(2+),fe(3+)) diiron site of desulfovibrio vulgaris rubrerythrin (rbr(mv)) were determined by continuous wave (cw) and pulsed endor spectroscopy at 35ghz. (14)n endor evidence indicates that a nitrogen is bound only to the fe(2+) ion of the mixed-valent site. assuming that this nitrogen is from his131ndelta, the same one that furnishes an iron ligand in the crystal structure of the diferric site, the ... | 2003 | 12755623 |
| cloning and expression of the superoxide dismutase gene from the obligate anaerobic bacterium desulfovibrio vulgaris (miyazaki f). | we identified the sod gene in the obligate anaerobic bacterium desulfovibrio vulgaris (miyazaki f) and constructed a high-level expression system in escherichia coli. a 2.6-kbp dna fragment isolated from d. vulgaris (miyazaki f) by double digestion with ecori and smai contained the sod gene and part of another open reading frame. the amino acid sequence deduced from the sod gene, which was composed of 238 amino acid residues, showed high homogeneity with iron-containing sod (fe-sod) and predicte ... | 2003 | 12761175 |
| docking and electron transfer studies between rubredoxin and rubredoxin:oxygen oxidoreductase. | the interaction and electron transfer (et) between rubredoxin (rd) and rubredoxin:oxygen oxidoreductase (roo) from desulfovibrio gigas is studied by molecular modelling techniques. experimental kinetic assays using recombinant proteins show that the rd reoxidation by roo displays a bell-shaped dependence on ionic strength, suggesting a non-trivial electrostatic dependence of the interaction between these two proteins. rigid docking studies reveal a prevalence for rd to interact, in a very specif ... | 2003 | 12761668 |
| reduced hybrid cluster proteins (hcp) from desulfovibrio desulfuricans atcc 27774 and desulfovibrio vulgaris (hildenborough): x-ray structures at high resolution using synchrotron radiation. | the hybrid cluster proteins from the sulfate reducing bacteria desulfovibrio desulfuricans atcc 27774 ( dd) and desulfovibrio vulgaris strain hildenborough ( dv) have been isolated and crystallized anaerobically. in each case, the protein has been reduced with dithionite and the crystal structure of the reduced form elucidated using x-ray synchrotron radiation techniques at 1.25 a and 1.55 a resolution for dd and dv, respectively. although the overall structures of the proteins are unchanged upo ... | 2003 | 12764602 |
| spectroscopic characterization of the [fe(his)(4)(cys)] site in 2fe-superoxide reductase from desulfovibrio vulgaris. | the electronic and vibrational properties of the [fe(his)(4)(cys)] site (center ii) responsible for catalysis of superoxide reduction in the two-iron superoxide reductase (2fe-sor) from desulfovibrio vulgaris have been investigated using the combination of epr, resonance raman, uv/visible/near-ir absorption, cd, and vtmcd spectroscopies. deconvolution of the spectral contributions of center ii from those of the [fe(cys)(4)] site (center i) has been achieved by parallel investigations of the c13s ... | 2003 | 12764688 |
| inhibition of bacterial u(vi) reduction by calcium. | the rapid kinetics of bacterial u(vi) reduction and low solubility of uraninite (uo2,cr) make this process an attractive option for removing uranium from groundwater. nevertheless, conditions that may promote or inhibit u(vi) reduction are not well-defined. recent descriptions of ca-uo2-co3 complexes indicate that these species may dominate the aqueous speciation of u(vi) in many environments. we monitored the bacterial reduction of u(vi) in bicarbonate-buffered solution in the presence and abse ... | 2003 | 12775057 |
| response of a strict anaerobe to oxygen: survival strategies in desulfovibrio gigas. | the biochemical response to oxygen of the strictly anaerobic sulfate-reducing bacterium desulfovibrio gigas was studied with the goal of elucidating survival strategies in oxic environments. cultures of d. gigas on medium containing lactate and sulfate were exposed to oxygen (concentration 5-120 micro m). growth was fully inhibited by oxygen, but the cultures resumed growth as soon as they were shifted back to anoxic conditions. following 24 h exposure to oxygen the growth rate was as high as 70 ... | 2003 | 12777491 |
| desulfovibrio desulfuricans bacteremia and review of human desulfovibrio infections. | one case of primary desulfovibrio desulfuricans bacteremia in an immunocompetent man is presented, and 15 other reported cases are reviewed. while most isolates have not been identified to the species level, desulfovibrio fairfieldensis and d. desulfuricans have been associated with incidents of bacteremia and d. vulgaris has been associated with intra-abdominal infections. in vitro studies suggest that empirical therapy with either imipenem or metronidazole should be considered. | 2003 | 12791922 |
| a novel membrane-bound ech [nife] hydrogenase in desulfovibrio gigas. | in the present study, we report the identification of an operon with six coding regions for a multisubunit membrane-bound [nife] hydrogenase in the genome of desulfovibrio gigas. sequence analysis of the deduced polypeptides reveals a high similarity to subunits of proteins belonging to the family of ech hydrogenases. the operon is organised similarly to the operon coding for the ech hydrogenase from methanosarcina barkeri, suggesting that both encode very similar hydrogenases. expression of the ... | 2003 | 12804572 |
| valid publication of the genus name thermodesulfobacterium and the species names thermodesulfobacterium commune (zeikus et al. 1983) and thermodesulfobacterium thermophilum (ex desulfovibrio thermophilus rozanova and khudyakova 1974). opinion 71. | the judicial commission of the international committee on systematics of prokaryotes decided that the date of valid publication of the genus name thermodesulfobacterium and of the species names thermodesulfobacterium commune and thermodesulfobacterium thermophilum is 1995. thermodesulfobacterium mobile rozanova and pivovarova 1988 is an illegitimate, later synonym of thermophilum. | 2003 | 12807221 |
| nitrite reductase activity of sulphate-reducing bacteria prevents their inhibition by nitrate-reducing, sulphide-oxidizing bacteria. | sulphate-reducing bacteria (srb) can be inhibited by nitrate-reducing, sulphide-oxidizing bacteria (nr-sob), despite the fact that these two groups are interdependent in many anaerobic environments. practical applications of this inhibition include the reduction of sulphide concentrations in oil fields by nitrate injection. the nr-sob thiomicrospira sp. strain cvo was found to oxidize up to 15 mm sulphide, considerably more than three other nr-sob strains that were tested. sulphide oxidation inc ... | 2003 | 12823193 |
| toxicity of al to desulfovibrio desulfuricans. | the toxicity of al to desulfovibrio desulfuricans g20 was assessed over a period of 8 weeks in a modified lactate c medium buffered at four initial phs (5.0, 6.5, 7.2, and 8.3) and treated with five levels of added al (0, 0.01, 0.1, 1.0, and 10 mm). at ph 5, cell population densities decreased significantly and any effect of al was negligible compared to that of the ph. at phs 6.5 and 7.2, the cell population densities increased by 30-fold during the first few days and then remained stable for s ... | 2003 | 12839782 |
| evidence for chimeric sequences formed during random arbitrarily primed pcr. | chimeric sequences were observed to occur abundantly (48% of clones) during random arbitrarily primed polymerase chain reaction (rap-pcr) experiments designed to examine differential expression of genes involved in metal resistance in sulfate-reducing bacteria (srb). some of the chimeric sequences were composed of sequence from a gene differentially expressed under the imposed conditions and a sequence of the 16s or 23s rrna gene. the remainder were composed of two rrna sequences. experiments us ... | 2003 | 12842491 |
| hydrogenases in sulfate-reducing bacteria function as chromium reductase. | the ability of sulfate-reducing bacteria (srb) to reduce chromate vi has been studied for possible application to the decontamination of polluted environments. metal reduction can be achieved both chemically, by h(2)s produced by the bacteria, and enzymatically, by polyhemic cytochromes c(3). we demonstrate that, in addition to low potential polyheme c-type cytochromes, the ability to reduce chromate is widespread among [fe], [nife], and [nifese] hydrogenases isolated from srb of the genera desu ... | 2003 | 12861426 |
| gene expression analysis of energy metabolism mutants of desulfovibrio vulgaris hildenborough indicates an important role for alcohol dehydrogenase. | comparison of the proteomes of the wild-type and fe-only hydrogenase mutant strains of desulfovibrio vulgaris hildenborough, grown in lactate-sulfate (ls) medium, indicated the near absence of open reading frame 2977 (orf2977)-coded alcohol dehydrogenase in the hyd mutant. hybridization of labeled cdna to a macroarray of 145 pcr-amplified d. vulgaris genes encoding proteins active in energy metabolism indicated that the adh gene was among the most highly expressed in wild-type cells grown in ls ... | 2003 | 12867442 |
| density functional theory investigation of the active site of [fe]-hydrogenases: effects of redox state and ligand characteristics on structural, electronic, and reactivity properties of complexes related to the [2fe]h subcluster. | the effects of redox state and ligand characteristics on structural, electronic, and reactivity properties of complexes related to the [2fe](h) subcluster of [fe]-hydrogenases have been investigated by dft calculations and compared with experimental and theoretical data obtained investigating both the enzyme and synthetic model complexes. our results show that fe(ii)fe(ii) species characterized by oh or h(2)o groups terminally coordinated to the iron atom distal to the terminal sulfur ligand (fe ... | 2003 | 12870970 |
| a sulfate-reducing bacterium that can detoxify u(vi) and obtain energy via nitrate reduction. | bacterial strain ufz b 490, which was isolated from a uranium dump and is closely related to desulfovibrio vulgaris oxamicus(t) (dsm 1925(t)), is able to detoxify u(vi) in aqueous media. in experiments reported here, u(vi) was used as an electron acceptor and lactate as electron donor. the reduction of soluble u(vi) to solid u(iv) (uraninite) did not provide energy for growth of strain ufz b 490. however, the isolate is able to grow when supplied with nitrate as sole electron acceptor and nitrog ... | 2003 | 12872316 |
| sulphate-reducing bacteria, palladium and the reductive dehalogenation of chlorinated aromatic compounds. | the surfaces of cells of desulfovibrio desulfuricans, desulfovibrio vulgaris and a new strain, desulfovibrio sp. 'oz-7' were used to manufacture a novel bioinorganic catalyst via the reduction of pd(ii) to pd(0) at the cell surface using hydrogen as the electron donor. the ability of the palladium coated (palladised) cells to reductively dehalogenate chlorophenol and polychlorinated biphenyl species was demonstrated. dried, palladised cells of d. desulfuricans, d. vulgaris and desulfovibrio sp. ... | 2003 | 12877464 |
| the cytochrome c3-[fe]-hydrogenase electron-transfer complex: structural model by nmr restrained docking. | cytochrome c(3) (m(r) 13000) is a low redox potential cytochrome specific of the anaerobic metabolism in sulfate-reducing bacteria. this tetrahemic cytochrome is an intermediate between the [fe]-hydrogenase and the cytochrome hmc in desulfovibrio vulgaris hildenborough strain. the present work describes the structural model of the cytochrome c(3)-[fe]-hydrogenase complex obtained by nuclear magnetic resonance restrained docking. this model connects the distal cluster of the [fe]-hydrogenase to h ... | 2003 | 12885397 |
| kinetic behavior of desulfovibrio gigas aldehyde oxidoreductase encapsulated in reverse micelles. | we report the kinetic behavior of the enzyme aldehyde oxidoreductase (aor) from the sulfate reducing bacterium desulfovibrio gigas (dg) encapsulated in reverse micelles of sodium bis-(2-ethylhexyl) sulfosuccinate in isooctane using benzaldehyde, octaldehyde, and decylaldehyde as substrates. dg aor is a 200-kda homodimeric protein that catalyzes the conversion of aldehydes to carboxylic acids. ultrasedimentation analysis of dg aor-containing micelles showed the presence of 100-kda molecular weigh ... | 2003 | 12890482 |
| inhibiting mild steel corrosion from sulfate-reducing bacteria using antimicrobial-producing biofilms in three-mile-island process water. | biofilms were used to produce gramicidin s (a cyclic decapeptide) to inhibit corrosion-causing, sulfate-reducing bacteria (srb). in laboratory studies these biofilms protected mild steel 1010 continuously from corrosion in the aggressive, cooling service water of the amergen three-mile-island (tmi) nuclear plant, which was augmented with reference srb. the growth of both reference srb (gram-positive desulfosporosinus orientis and gram-negative desulfovibrio vulgaris) was shown to be inhibited by ... | 2004 | 12898064 |
| kinetics of the superoxide reductase catalytic cycle. | the steady state kinetics of a desulfovibrio (d.) vulgaris superoxide reductase (sor) turnover cycle, in which superoxide is catalytically reduced to hydrogen peroxide at a [fe(his)4(cys)] active site, are reported. a proximal electron donor, rubredoxin, was used to supply reducing equivalents from nadph via ferredoxin: nadp+ oxidoreductase, and xanthine/xanthine oxidase was used to provide a calibrated flux of superoxide. sor turnover in this system was well coupled, i.e. approximately 2o*2 red ... | 2003 | 12900405 |
| evaluation of arbitrarily primed pcr for typing of desulfovibrio desulfuricans strains. | arbitrarily primed polymerase chain reaction (ap-pcr) method was applied to the differentiation of 15 (soil and intestinal) desulfovibrio desulfuricans strains. the primer m 13, which is a core sequence of phage m 13, was found to be appropriate for the differentiation of isolates of this species. the analysis revealed characteristic band patterns for all of the examined strains of which two soil strains (dv-7 and dv-8) showed identical dna fingerprints. according to jaccard's coefficient, the s ... | 2003 | 12906391 |
| a novel membrane-bound respiratory complex from desulfovibrio desulfuricans atcc 27774. | in the anaerobic respiration of sulfate, performed by sulfate-reducing prokaryotes, reduction of the terminal electron acceptor takes place in the cytoplasm. the membrane-associated electron transport chain that feeds electrons to the cytoplasmic reductases is still very poorly characterized. in this study we report the isolation and characterization of a novel membrane-bound redox complex from desulfovibrio desulfuricans atcc 27774. this complex is formed by three subunits, and contains two hem ... | 2003 | 12907302 |
| diversity of bacteria and archaea in sulphate-reducing enrichment cultures inoculated from serial dilution of zostera noltii rhizosphere samples. | we have analysed the diversity of culturable sulphate-reducing bacteria (srb) in zostera noltii colonized sediments from bassin d'arcachon (france). four organic substrates have been tested as well as the combination of h2 and co2 to select for lithotrophic srb. all energy sources were supplied in parallel cultures that were amended with yeast extract plus nh4+ and prepared without a source of combined nitrogen, the latter to select for diazotrophic srb. the 10 different enrichment media were in ... | 2003 | 12919411 |
| preparation and x-ray crystallographic analysis of rubredoxin crystals from desulfovibrio gigas to beyond ultra-high 0.68 a resolution. | rubredoxin (d.g. rd), a small non-heme iron-sulfur protein shown to function as a redox coupling protein from the sulfate reducing bacteria desulfovibrio gigas, has been crystallized using the hanging-drop vapor diffusion method and macroseeding method. rubredoxin crystals diffract to an ultra-high resolution 0.68 a using synchrotron radiation x-ray, and belong to the space group p2(1) with unit-cell parameters a=19.44 a, b=41.24 a, c=24.10 a, and beta=108.46 degrees. the data set of single-wave ... | 2003 | 12927773 |
| mercury methylation independent of the acetyl-coenzyme a pathway in sulfate-reducing bacteria. | sulfate-reducing bacteria (srb) in anoxic waters and sediments are the major producers of methylmercury in aquatic systems. although a considerable amount of work has addressed the environmental factors that control methylmercury formation and the conditions that control bioavailability of inorganic mercury to srb, little work has been undertaken analyzing the biochemical mechanism of methylmercury production. the acetyl-coenzyme a (coa) pathway has been implicated as being key to mercury methyl ... | 2003 | 12957930 |
| bacterial diversity and sulfur cycling in a mesophilic sulfide-rich spring. | an artesian sulfide- and sulfur-rich spring in southwestern oklahoma is shown to sustain an extremely rich and diverse microbial community. laboratory incubations and autoradiography studies indicated that active sulfur cycling is occurring in the abundant microbial mats at zodletone spring. anoxygenic phototrophic bacteria oxidize sulfide to sulfate, which is reduced by sulfate-reducing bacterial populations. the microbial community at zodletone spring was analyzed by cloning and sequencing 16s ... | 2003 | 12957951 |
| crystal structure of dissimilatory sulfite reductase d (dsrd) protein--possible interaction with b- and z-dna by its winged-helix motif. | the crystal structure of dsrd from desulfovibrio vulgaris hildenborough has been determined at 1.2 a resolution. dsrd is in a dimeric form in the crystal, and five sulfate anions were located on the surface. the structure of dsrd comprises a winged-helix motif, which shows the highest structural homology to similar motifs found in z-dna binding proteins and some b-dna binding proteins. the core structure of the molecule is constructed by intramolecular interactions of hydrophobic residues, which ... | 2003 | 12962631 |
| [the instability of the characteristics of a strain of sporovibrio]. | | 1952 | 12976742 |
| [metabolism of amino acids and of amides by sulfate-reducing bacteria]. | | 1952 | 13017187 |
| cultivation of sulphate-reducing bacteria. | | 1953 | 13054644 |
| on the nutrition of desulphovibrio desulphuricans; a correction. | | 1953 | 13118091 |
| [the activity and the growth of anaerobic sulfate-reducing bacteria in semi-autotrophic cultures]. | | 1953 | 13124948 |
| presence of cytochrome in an obligate anaerobe. | | 1953 | 13140202 |
| [anaerobic degradation of pyruvates by enzymatic extract of desulfovibrio desulfuricans]. | | 1954 | 13141483 |
| [new species of sporovibrio: s. ferroxydans (n.sp.)]. | | 1954 | 13150588 |
| [role of phosphate in chemical synthesis in sulfate-reducing bacteria]. | | 1954 | 13161771 |
| pyruvate oxidation in desulphovibrio desulphuricans. | | 1954 | 13163003 |
| purification of hydrogenase from desulfovibrio desulfuricans. | | 1954 | 13172252 |
| dependence of sulphate reduction and oxygen utilization on a cytochrome in desulphovibrio. | | 1954 | 13198885 |
| [fermentation of pyruvic acid and dicarboxylic acids by anaerobic sulfate-reducing bacteria]. | | 1954 | 13199530 |
| [preliminary note on the anaerobic degradation of cysteine and cystine by sulfate-reducing bacteria]. | | 1954 | 13199531 |
| the release of oil from petroleum-bearing materials by sulfate-reducing bacteria. | | 1954 | 13229283 |
| cytochrome c3, a bifunctional haematohaematin. | | 1955 | 13276413 |
| the assay of soluble hydrogenase. | | 1955 | 13276416 |
| [not available]. | | 1956 | 13305034 |
| purification and properties of the hydrogenase of desulfovibrio desulfuricans. | | 1956 | 13315304 |
| not available | | 1956 | 13317133 |
| [not available]. | | 1955 | 13329606 |
| cytochrome c3 and desulphoviridin; pigments of the anaerobe desulphovibrio desulphuricans. | | 1956 | 13346018 |
| iron and the synthesis of cytochrome c3. | | 1956 | 13357726 |
| [cytochrome c3 reactivation of hydroxylamine- and nitrite-reductase of sulfate-reducing bacteria]. | | 1956 | 13365197 |
| fractionation of stable isotopes of sulfur by microorganisms and their role in deposition of native sulfur. | | 1957 | 13425528 |
| studies on thermophilic sulfate reducing bacteria. i. identification of sporovibrio desulfuricans as clostridium nigrificans. | | 1957 | 13428685 |
| on the osmotic behaviour of desulphovibrio desulphuricans. | | 1957 | 13439144 |
| sodium chloride and the growth of desulphovibrio desuplphuricans. | | 1957 | 13481319 |
| a method for the rapid cultivation of desulfovibrio aestuarii on filter membranes. | | 1958 | 13509654 |
| [the reduction of hydroxylamine with reference to the hydrogenase activity of desulfovibrio desulfuricans. i. activity of cells and extracts]. | | 1958 | 13535641 |
| [reduction of hydroxylamine by hydrogenase activity of desulfovibrio desulfuricans. ii. nature of the enzyme system & the electron carrier in the reaction]. | | 1958 | 13535733 |
| [coupling of the dehydrogenation of pyruvate to the reduction of nitrite in sulfate-reducing bacteria; nature of the hydrogen carrier]. | | 1958 | 13573708 |
| enzymic oxidation of carbon monoxide. | | 1958 | 13584419 |
| [purification of 4-aspartic acid decarboxylase of desulfovibrio desulfuricans]. | | 1958 | 13618250 |
| [nitrogen fixation by sulfatereducing bacteria; isolation and characterization of the active strains]. | | 1959 | 13627588 |
| [reduction of nitrite at the expense of molecular hydrogen by desulfovibrio desulfuricans and other bacterial species]. | | 1958 | 13629248 |
| a diagnostic reaction of desulphovibrio desulphuricans. | | 1959 | 13632776 |
| a new sulphate-reducing vibrio. | | 1959 | 13654719 |
| on sporulation in sulphate-reducing bacteria. | | 1961 | 13681407 |
| the sensitivity of mixed population of bacteria to inhibitors. i. the mechanism by which desulfovibrio desulfuricans protects ps. aeruginosa from the toxicity of mercurials. | | 1961 | 13685634 |
| biochemical studies on sulfate-reducing bacteria. ix. sulfite reductase. | | 1961 | 13718010 |
| purification and properties of the hydrogenase of desulfovibrio desulfuricans. | | 1961 | 13745271 |
| [study of the bacteriostatic and bactericidal power of some compounds on a pure strain of sulfate-reducing bacteria]. | | 1961 | 13758213 |
| energy coupling in desulfovibrio desulfuricans. | | 1960 | 13768804 |
| culture of desulphovibrio desulphuricans. | | 1960 | 13791397 |
| a sulphate-reducing bacterium from the sheep rumen. | | 1960 | 13811147 |