| tolerance of listeria monocytogenes to quaternary ammonium sanitizers is mediated by a novel efflux pump encoded by emre. | a novel genomic island (lgi1) was discovered in listeria monocytogenes isolates responsible for the deadliest listeriosis outbreak in canada, in 2008. to investigate the functional role of lgi1, the outbreak strain 08-5578 was exposed to food chain-relevant stresses, and the expression of 16 lgi1 genes was measured. lgi1 genes with putative efflux (l. monocytogenes emre [emrelm]), regulatory (lmo1851), and adhesion (sel1) functions were deleted, and the mutants were exposed to acid (hcl), cold ( ... | 2015 | 26590290 |
| determination of evolutionary relationships of outbreak-associated listeria monocytogenes strains of serotypes 1/2a and 1/2b by whole-genome sequencing. | we used whole-genome sequencing to determine evolutionary relationships among 20 outbreak-associated clinical isolates of listeria monocytogenes serotypes 1/2a and 1/2b. isolates from 6 of 11 outbreaks fell outside the clonal groups or "epidemic clones" that have been previously associated with outbreaks, suggesting that epidemic potential may be widespread in l. monocytogenes and is not limited to the recognized epidemic clones. pairwise comparisons between epidemiologically related isolates wi ... | 2015 | 26590286 |
| processing-dependent and clonal contamination patterns of listeria monocytogenes in the cured ham food chain revealed by genetic analysis. | the quantitative and qualitative patterns of environmental contamination by listeria monocytogenes were investigated in the production chain of dry-cured parma ham. standard arrays of surfaces were sampled in processing facilities during a single visit per plant in the three compartments of the food chain, i.e., ham production (19 plants) and postproduction, which was divided into deboning (43 plants) and slicing (25 plants) steps. the numbers of sampled surfaces were 384 in ham production, with ... | 2015 | 26590278 |
| listeria monocytogenes: strain heterogeneity, methods, and challenges of subtyping. | listeria monocytogenes is a food-borne bacterial pathogen that is associated with 20% to 30% case fatality rate. l. monocytogenes is a genetically heterogeneous species, with a small fraction of strains (serotypes 1/2a, 1/2b, 4b) implicated in human listeriosis. monitoring and source tracking of l. monocytogenes involve the use of subtyping methods, with the performance of genetic-based methods found to be superior to phenotypic-based ones. various methods have been used to subtype l. monocytoge ... | 2015 | 26588067 |
| smad4 represses the generation of memory-precursor effector t cells but is required for the differentiation of central memory t cells. | the transcriptional regulation underlying the differentiation of cd8(+) effector and memory t cells remains elusive. here, we show that 18-month-old mice lacking the transcription factor smad4 (homolog 4 of mothers against decapentaplegic, drosophila), a key intracellular signaling effector for the tgf-β superfamily, in t cells exhibited lower percentages of cd44(hi)cd8(+) t cells. to explore the role of smad4 in the activation/memory of cd8(+) t cells, 6- to 8-week-old mice with or without smad ... | 2015 | 26583325 |
| economic cost of a listeria monocytogenes outbreak in canada, 2008. | estimates of the economic costs associated with foodborne disease are important to inform public health decision-making. in 2008, 57 cases of listeriosis and 24 deaths in canada were linked to contaminated delicatessen meat from one meat processing plant. costs associated with the cases (including medical costs, nonmedical costs, and productivity losses) and those incurred by the implicated plant and federal agencies responding to the outbreak were estimated to be nearly $242 million canadian do ... | 2015 | 26583272 |
| health trajectories reveal the dynamic contributions of host genetic resistance and tolerance to infection outcome. | resistance and tolerance are two alternative strategies hosts can adopt to survive infections. both strategies may be genetically controlled. to date, the relative contribution of resistance and tolerance to infection outcome is poorly understood. here, we use a bioluminescent listeria monocytogenes (lm) infection challenge model to study the genetic determination and dynamic contributions of host resistance and tolerance to listeriosis in four genetically diverse mouse strains. using convention ... | 2015 | 26582028 |
| a detailed view of the intracellular transcriptome of listeria monocytogenes in murine macrophages using rna-seq. | listeria monocytogenes is a bacterial pathogen and causative agent for the foodborne infection listeriosis, which is mainly a threat for pregnant, elderly, or immunocompromised individuals. due to its ability to invade and colonize diverse eukaryotic cell types including cells from invertebrates, l. monocytogenes has become a well-established model organism for intracellular growth. almost 10 years ago, we and others presented the first whole-genome microarray-based intracellular transcriptome o ... | 2015 | 26579105 |
| growth and inactivation of salmonella enterica and listeria monocytogenes in broth and validation in ground pork meat during simulated home storage abusive temperature and home pan-frying. | ground pork meat with natural microbiota and inoculated with low initial densities (1-10 or 10-100 cfu/g) of salmonella enterica or listeria monocytogenes was stored under abusive temperature at 10°c and thermally treated by a simulated home pan-frying procedure. the growth and inactivation characteristics were also evaluated in broth. in ground pork meat, the population of s. enterica increased by less than one log after 12-days of storage at 10°c, whereas l. monocytogenes increased by 2.3 to 2 ... | 2015 | 26579079 |
| structure of the bacterial cell division determinant gpsb and its interaction with penicillin-binding proteins. | each bacterium has to co-ordinate its growth with division to ensure genetic stability of the population. consequently, cell division and growth are tightly regulated phenomena, albeit different bacteria utilise one of several alternative regulatory mechanisms to maintain control. here we consider gpsb, which is linked to cell growth and division in gram-positive bacteria. δgpsb mutants of the human pathogen listeria monocytogenes show severe lysis, division and growth defects due to distortions ... | 2016 | 26575090 |
| autophagy regulates t lymphocyte proliferation through selective degradation of the cell-cycle inhibitor cdkn1b/p27kip1. | the highly conserved cellular degradation pathway, macroautophagy, regulates the homeostasis of organelles and promotes the survival of t lymphocytes. previous results indicate that atg3-, atg5-, or pik3c3/vps34-deficient t cells cannot proliferate efficiently. here we demonstrate that the proliferation of atg7-deficient t cells is defective. by using an adoptive transfer and listeria monocytogenes (lm) mouse infection model, we found that the primary immune response against lm is intrinsically ... | 2015 | 26569626 |
| a delayed cerebral vasospasm with infarction is secondary to listeria monocytogenes meningitis: mri and mra are diagnostically useful. | among patients with bacterial meningitis, a cerebral vasospasm typically occurs during the acute phase. we experienced a case of delayed cerebral vasospasm with infarction that was secondary to listeria monocytogenes meningitis. an 82-year-old woman with listeria monocytogenes meningitis, whose symptoms had been improving after the initiation of antibacterial therapy, fell into a coma on day 15 and developed generalized seizure. magnetic resonance imaging (mri) and mr angiography (mra) indicated ... | 2015 | 26568013 |
| pectin and xyloglucan influence the attachment of salmonella enterica and listeria monocytogenes to bacterial cellulose-derived plant cell wall models. | minimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. these pathogens must attach to the produce in order to be transmitted. cut surfaces of produce that expose cell walls are particularly vulnerable. little is known about the roles that different structural components (cellulose, pectin, and xyloglucan) of plant cell walls play in the attachment of foodborne bacterial pathogens. using bacterial cellulose-derived plant cell wall models ... | 2015 | 26567310 |
| adam17 controls il-6 signaling by cleavage of the murine il-6rα from the cell surface of leukocytes during inflammatory responses. | the cytokine il-6 is part of a regulatory signaling network that controls immune responses. il-6 binds either to the membrane-bound il-6 receptor-α (classic signaling) or to the soluble il-6 receptor-α (trans-signaling) to initiate signal transduction via gp130 activation. because classic and trans-signaling of il-6 fulfill different tasks during immune responses, controlled shedding of the membrane-bound il-6 receptor-α from the surface of immune cells can be considered a central regulator of i ... | 2016 | 26561568 |
| an interactomic approach for identification of putative drug targets in listeria monocytogenes. | a wide variety of human population is infected with listeria monocytogenes, which causes listeriosis, a deadly disease with mortality rate of about 30%. the major hindrance to cure listeriosis is the unavailability of specific or selectable drug targets. at present, antibiotics used to cure the disease are not specific and insufficient to manage the disease efficiently. therefore, in order to search specific drugs, here, we used interactome analysis to search specific drug targets which may prov ... | 2015 | 26561317 |
| antimicrobial and antioxidant activities of enterococcus species isolated from meat and dairy products. | abstractlactic acid bacteria (lab) have an important role in a great variety of fermented foods. in addition to their contribution to sensory characteristics, they enhance food preservation and can be used as probiotics. in this study, the antimicrobial and antioxidant activities of culture supernatants and cell free extracts of 16 lab isolated from meat and dairy products were investigated. the bacterial were identified by 16s rrna sequencing. genbank blast analysis revealed that all the isolat ... | 2015 | 26560661 |
| prevalence of foodborne pathogens in freshwater fish in latvia. | the aim of this study was to detect the prevalence of salmonella spp., listeria monocytogenes, and yersinia enterocolitica in freshwater fish in latvia. in total, 235 samples, including freshly caught fish from fives lakes (n = 129) and fish from retail markets (n = 106), were collected from april 2014 to december 2014 in latvia. samples were tested according to international organization for standardization methods. no salmonella spp. were found in fresh fish from lakes or in commercially avail ... | 2015 | 26555535 |
| predominance and distribution of a persistent listeria monocytogenes clone in a commercial fresh mushroom processing environment. | a longitudinal study was conducted to determine the prevalence of listeria spp. in a commercial fresh mushroom slicing and packaging environment. samples were collected at three different sampling periods within a 13-month time interval. of the 255 environmental samples collected, 18.8% tested positive for l. monocytogenes, 4.3% for l. innocua, and 2.0% for l. grayi. l. monocytogenes was most often found on wet floors within the washing and slicing and packaging areas. each of the 171 l. monocyt ... | 2015 | 26555522 |
| disease burden of invasive listeriosis and molecular characterization of clinical isolates in taiwan, 2000-2013. | the information about disease burden and epidemiology of invasive listeriosis in asia is scarce. from 2000 to 2013, a total of 338 patients with invasive listeriosis (bacteremia, meningitis, and peritonitis) were treated at four medical centers in taiwan. the incidence (per 10,000 admissions) of invasive listeriosis increased significantly during the 14-year period among the four centers (0.15 in 2000 and >1.25 during 2010-2012) and at each of the four medical centers. among these patients, 45.9 ... | 2015 | 26555445 |
| listeria monocytogenes associated kerato-conjunctivitis in four horses in norway. | listeria monocytogenes has been reported to cause various infectious diseases in both humans and animals. more rarely, ocular infections have been reported. to our knowledge, only two cases of listeria keratitis have been described in horses. we report kerato-conjunctivitis in four norwegian horses associated with l. monocytogenes. clinically, all cases were presented with recurrent unilateral kerato-conjunctivitis. l. monocytogenes bacteria were isolated from swab samples from all cases, and cy ... | 2015 | 26552393 |
| induced systemic listeriosis in alectoris chukar chicks: clinical, histopathological and microbiological findings. | 1. systemic listeriosis was induced in 14-d-old chukar partridge chicks, alectoris chukar, by intravenous injection of a suspension containing 10(6) cfu/ml of viable listeria monocytogenes organisms to study the course of infection. 2. septicaemic and encephalitic forms of listeriosis were observed in all birds. infection resulted in a fever response 8-h post-inoculation. disease rapidly developed over a 24-h period with decreased activity, lethargy, ruffled feathers, huddling, listlessness, ina ... | 2015 | 26551997 |
| mir-150 regulates differentiation and cytolytic effector function in cd8+ t cells. | micrornas regulate most mammalian genes, and they control numerous aspects of immune system development and function. their precise roles in the cd8+ t cell response, however, remain unclear. in this report, we show that in the absence of the microrna mir-150, cd8+ t cells fail to undergo robust expansion and differentiation into short-lived terminal effector cells in response to primary infection with listeria monocytogenes or vaccinia virus. notably, even after transitioning into the memory po ... | 2015 | 26549197 |
| the opposite effects of dna and protein components of listeria monocytogenes and toxoplasma gondii on immunologic characteristics of dendritic cells. | the innate immune system utilizes pattern recognition receptors (prrs) to recognize microbes. pathogens contain various molecules with diverse effects on immune response. in this study, we evaluated the effect of dna and protein components derived from two intracellular microorganisms including listeria monocytogenes (l. monocytogenes) and toxoplasma gondii (t. gondii) on dendritic cells (dcs) activation and ensuing adaptive immune responses. dna and protein components of l. monocytogenes and t. ... | 2015 | 26546901 |
| up-regulation of cyld enhances listeria monocytogenes induced apoptosis in thp-1 cells. | listeria monocytogenes (lm), a facultative anaerobic gram-positive bacterium, causes listeriosis. immune cell apoptosis is considered to be one pathogenic factor for listeriosis. as a deubiquitinase, cyld is an important regulator both in innate immune response and apoptosis by negatively modulating nf-κb pathway. however the role of cyld in lm induced apoptosis remains unclear. here we found that cyld is significantly up-regulated in macrophages upon its infection. there is a moderate decrease ... | 2016 | 26545866 |
| foodborne pathogens prevention and sensory attributes enhancement in processed cheese via flavoring with plant extracts. | cheese contaminations with foodborne bacterial pathogens, and their health outbreaks, are serious worldwide problems that could happen from diverse sources during cheese production or storage. plants, and their derivatives, were always regarded as the potential natural and safe antimicrobial alternatives for food preservation and improvement. the extracts from many plants, which are commonly used as spices and flavoring agents, were evaluated as antibacterial agents against serious foodborne pat ... | 2015 | 26540146 |
| survival of unstressed and acid-, cold-, and starvation-stress-adapted listeria monocytogenes in ham extract with hops beta acids and consumer acceptability of hba on ready-to-eat ham. | the efficacy of hops beta acids (hba) against unstressed and stress-adapted listeria monocytogenes in ham extract and the consumers' acceptability of hba on ready-to-eat (rte) hams were investigated. unstressed or acid-, cold-, or starvation-stress-adapted l. monocytogenes was inoculated (1.3-1.5 log cfu/ml) into 10% ham extract, without (control) or with hba (4.44 or 10.0 µg/ml). survival/growth of the pathogen during storage (7.2 °c, 26 days) was monitored periodically. sensory evaluation (30 ... | 2015 | 26539527 |
| impact of moderate heat, carvacrol, and thymol treatments on the viability, injury, and stress response of listeria monocytogenes. | the microbial safety and stability of minimally processed foods are based on the application of combined preservative factors. since microorganisms are able to develop adaptive networks to survive under conditions of stress, food safety may be affected, and therefore understanding of stress adaptive mechanisms plays a key role in designing safe food processing conditions. in the present study, the viability and the sublethal injury of listeria monocytogenes exposed to moderate heat (55 °c) and/o ... | 2015 | 26539510 |
| twenty years of listeria in brazil: occurrence of listeria species and listeria monocytogenes serovars in food samples in brazil between 1990 and 2012. | listeria spp. isolated from different food products and collected from 12 brazilian states were sent to the laboratory of bacterial zoonoses (oswaldo cruz institute, brazil) for identification. the aims of this study were to characterize these isolates, from 1990 to 2012, by using biochemical, morphological, and serotyping tests, and to analyze the distribution of l. monocytogenes serotypes on different food products and geographical locations. serotyping was performed using polyclonal somatic a ... | 2015 | 26539507 |
| molecular epidemiology of invasive listeriosis due to listeria monocytogenes in a spanish hospital over a nine-year study period, 2006-2014. | we investigated the pathogenicity, invasiveness, and genetic relatedness of 17 clinical listeria monocytogenes stains isolated over a period of nine years (2006-2014). all isolates were phenotypically characterised and growth patterns were determined. the antimicrobial susceptibility of l. monocytogenes isolates was determined in e-tests. invasion assays were performed with epithelial hela cells. finally, l. monocytogenes isolates were subtyped by pfge and mlst. all isolates had similar phenotyp ... | 2015 | 26539467 |
| multiplex pcr serogrouping of listeria monocytogenes isolated in japan. | pcr serogrouping methods were used to examine strains of l. monocytogenes isolated in japan. among 187 strains, 99.5% were classified into 4 pcr serogroups corresponding to conventional serotypes. only one isolate had a new pcr profile, which may be a variant of serogroup ivb. | 2016 | 26537550 |
| highly invasive listeria monocytogenes strains have growth and invasion advantages in strain competition. | multiple listeria monocytogenes strains can be present in the same food sample; moreover, infection with more than one l. monocytogenes strain can also occur. in this study we investigated the impact of strain competition on the growth and in vitro virulence potential of l. monocytogenes. we identified two strong competitor strains, whose growth was not (or only slightly) influenced by the presence of other strains and two weak competitor strains, which were outcompeted by other strains. cell co ... | 2015 | 26529510 |
| comparative evaluation of veriflow® listeria monocytogenes to usda and aoac culture based methods for the detection of listeria monocytogenes in food. | veriflow® listeria monocytogenes (lm) is a molecular based assay for the presumptive detection of listeria monocytogenes from environmental surfaces, dairy, and ready-to-eat (rte) food matrixes (hot dogs and deli meat). the assay utilizes a pcr detection method coupled with a rapid, visual, flow-based assay that develops in 3 min post pcr amplification and requires only 24 h of enrichment for maximum sensitivity. the veriflow lm system eliminates the need for sample purification, gel electrophor ... | 2015 | 26525251 |
| method modification of the thermo scientific suretect listeria monocytogenes assay for raw meat, dairy, produce, and seafood. | the thermo scientific™ suretect™ listeria monocytogenes assay is a real-time pcr assay for the detection of listeria monocytogenes in food and environmental samples, which was certified during 2013 by the aoac research institute (ri) as performance tested method(sm) (ptm) 061302 for a representative range of key food matrixes and production surfaces. this report details the method modification study, which was conducted during 2014, using the aoac-ri ptm program to extend the validated matrix cl ... | 2015 | 26525250 |
| biofilm formation of the l. monocytogenes strain 15g01 is influenced by changes in environmental conditions. | listeria monocytogenes 15g01, a strain belonging to the persistent pulsotype 5132, was isolated from a seafood processing plant in new zealand. simple monoculture assays using crystal violet staining showed good biofilm formation for this strain and it was therefore chosen to be further investigated in regard to its biofilm forming ability. to evaluate its behaviour in different conditions commonly encountered in food processing environments, biofilm assays and growth studies were performed usin ... | 2015 | 26524221 |
| tracing sources of listeria contamination in traditional italian cheese associated with a us outbreak: investigations in italy. | in 2012 a us multistate outbreak of listeriosis was linked to ricotta salata imported from italy, made from pasteurized sheep's milk. sampling activities were conducted in italy to trace the source of listeria monocytogenes contamination. the cheese that caused the outbreak was produced in a plant in apulia that processed semi-finished cheeses supplied by five plants in sardinia. during an 'emergency sampling', 179 (23·6%) out of 758 end-products tested positive for l. monocytogenes, with concen ... | 2016 | 26522501 |
| effect of x-ray treatments on escherichia coli o157:h7, listeria monocytogenes, shigella flexneri, salmonella enterica and inherent microbiota on whole mangoes. | the aims of this investigation were to; (i) study the effect of x-ray treatments in reducing escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri on whole mangoes, and (ii) study the effect of x-ray treatments on microflora counts (mesophilic counts, psychrotrophic counts and yeast and mould counts) of whole mangoes during storage at ambient temperature (22°c) for 30 days. a mixture of three strains of each test organism was spot inoculated (100 μl; approx. ... | 2016 | 26518738 |
| listeria monocytogenes isolates from food and food environment harbouring tetm and ermb resistance genes. | listeria monocytogenes is a foodborne pathogen that has become an important cause of human and animal diseases worldwide. the purpose of this study was to evaluate the serotypes, virulence potential, antimicrobial resistance profile, and genetic relationships of 50 l. monocytogenes isolates from food and food environment in southern brazil. in this study, the majority of l. monocytogenes isolates belonged to the serotypes 1/2b (42%) and 4b (26%), which are the main serotypes associated with huma ... | 2016 | 26518475 |
| synergistic effect of copper and low temperature over listeria monocytogenes. | the capacity to grow at low temperatures has allowed listeria monocytogenes to become one of the primary food pathogens to date, representing a major public health problem worldwide. several works have described the homeostatic response of l. monocytogenes under different copper (cu) treatments growing at mild temperature (30 °c). the aims of this report were to evaluate if changes in the external concentration of cu affected viability and cu homeostasis of l. monocytogenes growing at low temper ... | 2015 | 26515293 |
| efflux pump-mediated benzalkonium chloride resistance in listeria monocytogenes isolated from retail food. | in this study, efflux pump-mediated benzalkonium chloride (bc) resistance, including plasmid-encoded (qac protein family and bcrabc) and chromosome-borne efflux pumps, was investigated in listeria monocytogenes from retail food in china. among the 59 l. monocytogenes strains, 13 (22.0%) strains were resistant to bc. the pcr results showed that bcrabc was harbored by 2 of 13 bc resistant strains. however, none of the qac genes were detected among the 59 strains. the bcrabc was absent in both of t ... | 2016 | 26513255 |
| the intrinsic cephalosporin resistome of listeria monocytogenes in the context of stress response, gene regulation, pathogenesis and therapeutics. | intrinsic resistance to antibiotics is a serious therapeutic problem in the case of many bacterial species. the gram-positive human pathogen listeria monocytogenes is intrinsically resistant to broad spectrum cephalosporin antibiotics, which are commonly used in therapy of bacterial infections. besides three penicillin-binding proteins the intrinsic cephalosporin resistome of l. monocytogenes includes multidrug resistance transporter transporters, proteins involved in peptidoglycan biosynthesis ... | 2016 | 26509460 |
| a tail of two phages: genomic and functional analysis of listeria monocytogenes phages vb_lmos_188 and vb_lmos_293 reveal the receptor-binding proteins involved in host specificity. | the physical characteristics of bacteriophages establish them as viable candidates for downstream development of pathogen detection assays and biocontrol measures. to utilize phages for such purposes, a detailed knowledge of their host interaction mechanisms is a prerequisite. there is currently a wealth of knowledge available concerning gram-negative phage-host interaction, but little by comparison for gram-positive phages and listeria phages in particular. in this research, the lytic spectrum ... | 2015 | 26500641 |
| cd8 t cells enter the splenic t cell zones independently of ccr7, but the subsequent expansion and trafficking patterns of effector t cells after infection are dysregulated in the absence of ccr7 migratory cues. | ccr7 is an important chemokine receptor that regulates t cell trafficking and compartmentalization within secondary lymphoid organs. however, the t cell-intrinsic role of ccr7 during infection in the spleen is not well understood. this study was designed to understand how ccr7-dependent localization and migration of cd8(+) t cells in different compartments of the spleen affected the primary and recall responses after infection. to this end, we used adoptive transfer of naive ag-specific cd8 t ce ... | 2015 | 26500349 |
| the connection between persistent, disinfectant-resistant listeria monocytogenes strains from two geographically separate iberian pork processing plants: evidence from comparative genome analysis. | the aim of this study was to investigate the basis of the putative persistence of listeria monocytogenes in a new industrial facility dedicated to the processing of ready-to-eat (rte) iberian pork products. quaternary ammonium compounds, which included benzalkonium chloride (bac), were repeatedly used as surface disinfectants in the processing plant. clean and disinfected surfaces were sampled to evaluate if resistance to disinfectants was associated with persistence. of the 14 isolates obtained ... | 2015 | 26497458 |
| enhanced antimicrobial effect of organic acid washing against foodborne pathogens on broccoli by vacuum impregnation. | this study was undertaken to evaluate the effect of vacuum impregnation applied to the washing process for removal of salmonella typhimurium and listeria monocytogenes from broccoli surfaces. broccoli was inoculated with the two foodborne pathogens and treated with simple dipping washing or with vacuum impregnation in 2% malic acid for 5, 10, 20, or 30 min. there were two methods of vacuum impregnation: continuous and intermittent. after 30 min of 101.3 kpa (=14.7 psi, simple dipping), 61.3 kpa ... | 2016 | 26496412 |
| clonal clustering using 10-gene multilocus sequence typing reveals an association between genotype and listeria monocytogenes maximum growth rate in defined medium. | we used a 10-gene (10g) multilocus sequence typing scheme to investigate the diversity and phylogenetic distribution of 124 listeria monocytogenes strains across major lineages, major serotypes, and seven epidemic clones that have been previously associated with outbreaks. the 124 isolates proved to be diverse, with a total of 81 sequence types (10g-sts) belonging to 13 clonal complexes (ccs), where all sts of the same cc differ from one another in up to 3 of the 10 alleles (named as 10g-triple- ... | 2015 | 26495863 |
| ring-enhancing brain lesions: listeria monocytogenes abscesses. | | 2016 | 26494469 |
| outbreak of hospital-acquired gastroenteritis and invasive infection caused by listeria monocytogenes, finland, 2012. | during one week in july 2012, two patients from the same ward at the municipal hospital in vaasa, finland, were diagnosed with septicaemia caused by listeria monocytogenes. an outbreak investigation revealed eight concomitant cases of febrile gastroenteritis caused by l. monocytogenes on the same ward. median age of the cases was 82 years and median incubation time for listerial gastroenteritis was 21 h (range 9-107). an additional 10 cases of invasive listeriosis caused by the same outbreak str ... | 2016 | 26493730 |
| antagonizing peroxisome proliferator-activated receptor α activity selectively enhances th1 immunity in male mice. | females exhibit more robust th1 responses than males. our previous work suggested that this sex disparity is a consequence of higher activity of the androgen-induced gene peroxisome proliferator-activated receptor α (pparα) in male cd4(+) t cells. the objective of this study was to elucidate the cellular and molecular mechanism of how pparα inhibits th1 responses in male mice. in this study, we found that pparα functions within cd4(+) and cd8(+) t lymphocytes and nkt cells to negatively regulate ... | 2015 | 26491197 |
| synergism between carvacrol or thymol increases the antimicrobial efficacy of soy sauce with no sensory impact. | here, we examined the antimicrobial effects of soy sauce containing essential oils (eos) against escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes at 22°c and 4°c. to screen a variety of combined effects, soy sauce was mixed with six different eos (carvacrol, thymol, eugenol, trans-cinnamaldehyde, β-resorcylic acid, and vanillin), each at a concentration of 1mm for 10 min. none of the oils showed bactericidal activity when used alone. soy sauce combined with carvacrol ... | 2016 | 26490647 |
| the listeria monocytogenes core-genome sequence typer (lmcgst): a bioinformatic pipeline for molecular characterization with next-generation sequence data. | next-generation sequencing provides a powerful means of molecular characterization. however, methods such as single-nucleotide polymorphism detection or whole-chromosome sequence analysis are computationally expensive, prone to errors, and are still less accessible than traditional typing methods. here, we present the listeria monocytogenes core-genome sequence typing method for molecular characterization. this method uses a high-confidence core (hcc) genome, calculated to ensure accurate identi ... | 2015 | 26490433 |
| systemic tolerance mediated by melanoma brain tumors is reversible by radiotherapy and vaccination. | immune responses to antigens originating in the central nervous system (cns) are generally attenuated, as collateral damage can have devastating consequences. the significance of this finding for the efficacy of tumor-targeted immunotherapies is largely unknown. | 2016 | 26490306 |
| novel active surface prepared by embedded functionalized clays in an acrylate coating. | the research on a self-decontaminating surface has received significant attention because of the growth of pathogenic microorganisms on surfaces. in this study, a novel and simple technique for producing an active surface with antimicrobial functionality is demonstrated. a tethering platform was developed by grafting the biocide ampicillin (amp) to a nanoclay and dispersing the nanoclay in a uv-curable acrylate coating applied on polypropylene films as the substrate. a coupling agent, [3-(glycid ... | 2015 | 26488557 |
| prevalence, enumeration, and pheno- and genotypic characteristics of listeria monocytogenes isolated from raw foods in south china. | listeria monocytogenes is an important foodborne pathogen that can cause serious illness in immunocompromised individuals, pregnant women, the elderly, and newborns. the aim of this study was to: (i) evaluate the prevalence and contamination level [most probable number (mpn)] of l. monocytogenes in 567 retail raw foods (fishery products, n = 154; raw/fresh meat, n = 123; frozen foods, n = 110; edible fungi, n = 108; vegetables, n = 72) collected from south china and (ii) to gain further knowledg ... | 2015 | 26483765 |
| the first mollusk spätzle homolog gene in the clam, paphia undulate. | spätzle, is the only identified endogenous toll receptor ligand, plays a critical role in initiatinge innate immune responses and controlling dorsal-ventral axis formation in drosophila. here we identified the first spätzle gene homolog, pu-spz, in the marine mollusk paphia undulate. the full-length of pu-spz cdna is 1248 bp, including an open reading frame (orf) of 702 bp, a 5'-untranslated region (utr) of 26 bp and a 3'-utr of 203 bp. the orf encodes a 233-amino-acid protein with conserved dom ... | 2015 | 26477575 |
| occurrence of salmonella, listeria monocytogenes, and enterotoxigenic staphylococcus in goat milk from small and medium-sized farms located in minas gerais state, brazil. | consumption of goat milk has been increasing due to its nutritional characteristics and health benefits. therefore, assessment of the presence of foodborne pathogens in this product is critical to ensure its safety to consumers. the present study aimed to identify common foodborne pathogens in raw goat milk. fifty-three samples of raw goat milk from 11 farms were collected and cultured for the presence of salmonella spp. and listeria monocytogenes, as well as for enumeration and isolation of coa ... | 2015 | 26476939 |
| virus recovering from strawberries: evaluation of a skimmed milk organic flocculation method for assessment of microbiological contamination. | skimmed milk organic flocculation method was adapted, optimized and compared with polyethylene glycol (peg) precipitation and filtration methods for recovering viruses from a strawberry matrix. spiking experiments with norovirus genogroup ii genotype 4 (nov gii.4) and murine norovirus 1 (mnv-1) demonstrated that the organic flocculation method associated with a glycine elution buffer, filter bag and cetyltrimethylammonium bromide (ctab) showed a recovery percentage of 2.5 and 32 times higher tha ... | 2016 | 26476572 |
| genetic diversity, virulence potential and antimicrobial susceptibility of listeria monocytogenes recovered from different sources in india. | listeria monocytogenes isolates (n = 36) recovered from human and animal clinical cases and foods from different geographical regions of india were characterized using multiplex pcr-based serotyping, pulsed field gel electrophoresis (pfge), in vitro and in vivo pathogenicity tests and antibiogram profiling. multiplex pcr-based serotyping distributed l. monocytogenes isolates into 3 serogroups, of which 91.67% belonged to 4b, 4d, 4e serogroup, followed by 5.56% to 1/2a, 3a and 2.78% to 1/2b, 3b s ... | 2015 | 26476275 |
| outbreak of listeria monocytogenes in pheasants. | listeria monocytogenes is capable of infecting almost all animals. however, outbreaks of listeriosis are infrequent in birds. this report describes an outbreak of listeriosis in a small pheasant (phasianus colchicus) breeder farm with more than 2,000 pheasants from hubei province of the people's republic of china. the affected flock consisted of adult and young birds. approximately 300 young birds and a few adult birds were found dead within a few days of the onset of clinical signs. twenty-five ... | 2015 | 26476090 |
| macrophages mediate flagellin induced inflammasome activation and host defense in zebrafish. | the inflammasome is an innate immune complex whose rapid inflammatory outputs play a critical role in controlling infection; however, the host cells that mediate inflammasome responses in vivo are not well defined. using zebrafish larvae, we examined the cellular immune responses to inflammasome activation during infection. we compared the host responses with two listeria monocytogenes strains: wild type and lm-pyro, a strain engineered to activate the inflammasome via ectopic expression of flag ... | 2016 | 26468080 |
| antilisterial peptides released by enzymatic hydrolysis from grass carp proteins and activity on controlling l. monocytogenes inoculated in surimi noodle. | the primary objective of this study was to prepare the antilisterial peptides by enzymatic (pepsin, trypsin, protamex, neutrase, flavourzyme, papain, alcalase, and acid protease) hydrolysis of grass carp proteins with various degree of hydrolysis. the second objective was to evaluate the antilisterial activity of grass carp proteins hydrolysates (gcph) at differnet levels in the surimi noodle samples with or without boiling treatment inoculated with 10(4) cfu/g of listeria monocytogenes for stor ... | 2015 | 26467537 |
| comparative genomic analysis reveals that the 20k and 38k prophages in listeria monocytogenes serovar 4a strains lm850658 and m7 contribute to genetic diversity but not to virulence. | listeria monocytogenes is a foodborne pathogen of considerable genetic diversity with varying pathogenicity. initially, we found that the strain m7 was far less pathogenic than the strain lm850658 though both are serovar 4a strains belonging to the lineage iii. comparative genomic approaches were then attempted to decipher the genetic basis that might govern the strain-dependent pathotypes. there are 2,761 coding sequences of 100% nucleotide identity between the two strains, accounting for 95.7% ... | 2016 | 26464378 |
| growth of listeria monocytogenes within a caramel-coated apple microenvironment. | a 2014 multistate listeriosis outbreak was linked to consumption of caramel-coated apples, an unexpected and previously unreported vehicle for listeria monocytogenes. this outbreak was unanticipated because both the ph of apples (<4.0) and the water activity of the caramel coating (<0.80) are too low to support listeria growth. in this study, granny smith apples were inoculated with approximately 4 log10 cfu of l. monocytogenes (a cocktail of serotype 4b strains associated with the outbreak) on ... | 2015 | 26463161 |
| identification and characterization of species-specific nanobodies for the detection of listeria monocytogenes in milk. | listeria monocytogenes (lm), one of the eight species belonging to the genus listeria, is pathogenic for both humans and animals. in this study, two novel lm-specific clones, designated l5-78 and l5-79, were isolated from a phage display antibody library that was derived from the variable domain of heavy-chain antibodies (vhhs) of non-immunized alpaca. these two clones were expressed, purified, and characterized. results showed that both isolated vhhs recognize three serotypes (1/2a, 1/2b, and 4 ... | 2016 | 26456330 |
| comparison of multiple‑locus variable number tandem repeat analysis and pulsed field gel electrophoresis in molecular subtyping of listeria monocytogenes isolates from italian cheese. | subtyping of listeria monocytogenes strains is an essential epidemiological tool to trace contamination and determine evolutionary relationships among different strains. pulsed field gel electrophoresis (pfge) is the current gold standard method for listeria characterization. multiple-locus variable-number tandem repeat analysis (mlva) is a rapid subtyping method based on polymerase chain reaction (pcr) amplification that has been successfully developed for subtyping bacterial genera. the purpos ... | 2015 | 26455371 |
| effect of honokiol on exotoxin proteins listeriolysin o and p60 secreted by listeria monocytogenes. | listeria monocytogenes is considered one of the most important foodborne pathogens. the virulence-related proteins listeriolysin o (llo) and p60 are critical factors involved in listeria pathogenesis. in the present study, we investigated the effect of honokiol on llo and p60 secreted from l. monocytogenes. a listeriolysin assay was used to investigate the haemolytic activities of l. monocytogenes exposed to honokiol, and the secretion of llo and p60 was detected by immunoblot analysis. addition ... | 2015 | 26445991 |
| development of listeriabase and comparative analysis of listeria monocytogenes. | listeria consists of both pathogenic and non-pathogenic species. reports of similarities between the genomic content between some pathogenic and non-pathogenic species necessitates the investigation of these species at the genomic level to understand the evolution of virulence-associated genes. with listeria genome data growing exponentially, comparative genomic analysis may give better insights into evolution, genetics and phylogeny of listeria spp., leading to better management of the diseases ... | 2015 | 26444974 |
| drosophila melanogaster natural variation affects growth dynamics of infecting listeria monocytogenes. | we find that in a listeria monocytogenes/drosophila melanogaster infection model, l. monocytogenes grows according to logistic kinetics, which means we can measure both a maximal growth rate and growth plateau for the microbe. genetic variation of the host affects both of the pathogen growth parameters, and they can vary independently. because growth rates and ceilings both correlate with host survival, both properties could drive evolution of the host. we find that growth rates and ceilings are ... | 2015 | 26438294 |
| a case of listeria monocytogenes endophthalmitis with recurrent inflammation and novel management. | listeria monocytogenes is a rare cause of endogenous endophthalmitis. in the limited number of reported listeria endophthalmitis cases, visual acuity outcomes have been very poor. | 2015 | 26437938 |
| [listeria monocytogenes meningitis in immunocompetent children: unpasteurized cheese likely cause of infection]. | listeria meningoencephalitis is a rare condition, occurring mainly in immunocompromised patients. we present two cases of listeria monocytogenes meningoencephalitis in immunocompetent children, with successful treatment with betalactam/aminoglycoside combination. unpasteurized cheese was postulated as the source of infection. | 2015 | 26436795 |
| characterization of listeria monocytogenes isolated from human listeriosis cases in china. | | 2015 | 26421272 |
| use of e-beam radiation to eliminate listeria monocytogenes from surface mould cheese. | camembert and brie soft cheese varieties were subjected to e-beam irradiation as a sanitation treatment. the effects of treatments on microbiota and selected physicochemical properties were also studied. the absorbed doses required to meet the food safety objective (fso) according to eu and usda criteria for listeria monocytogenes were 1.27 and 2.59 kgy, respectively. the bacterial load, mainly lactic acid bacteria, was reduced by the treatment but injured cells were recovered during storage at ... | 2015 | 26415665 |
| [the stimulating effect of exometabolites of the marine microalgae phaeodactylum tricornutum bohlin on reproduction of listeria monocytogenes]. | the biological activity of ethyl acetate extract of exometabolites from the marine microalgae phaeodactylum tricornutum against a test culture of listeria monocytogenes 4b has been investigated. it was shown that the stimulating effect of algal exometabolites on the growth of pathogenic bacteria increased maximally by 98.3% on day 6 of the cultivation, if the active substances comprising the culture liquid of p. tricornutum were separated by successive extractions with solvents in order of incre ... | 2016 | 26415278 |
| behaviour of listeria monocytogenes and escherichia coli o157:h7 during the cheese making of traditional raw-milk cheeses from italian alps. | the behaviour of listeria monocytogenes and escherichia coli o157:h7 was studied during the manufacture and ripening of two traditional italian alps cheeses. each cheese type was manufactured in a pilot plan from raw cow milk (without the addition of starter cultures) artificially inoculated with l. monocytogenes and e. coli o157:h7 to a final concentration of about 4 log cfu/ml. the pathogens were enumerated throughout the cheese making and ripening processes to study their behaviour. when the ... | 2015 | 27800408 |
| characterization of epidemic clones of listeria monocytogenes serotype 4b isolated from humans and meat products in brazil. | listeria monocytogenes is an important foodborne pathogen and the 4b serotype is responsible for many cases of human listeriosis reported in brazil. several listeriosis outbreaks worldwide have involved a small number of well-defined clonal groups, designated as epidemic clones (ecs). | 2015 | 26409737 |
| behavior of listeria monocytogenes in sliced ready-to-eat meat products packaged under vacuum or modified atmosphere conditions. | the objective of this research was to determine the behavior of listeria monocytogenes in three types of sliced ready-to-eat meat products packaged under vacuum or modified atmosphere conditions and stored at three temperatures. slices of about 25 g of chorizo (a fermented dry pork sausage), jamón (cured ham), and cecina (a salted, dried beef product) were inoculated with l. monocytogenes nctc 11994. slices were packaged in a vacuum or in a modified atmosphere (20% co2, 80% n2). after packaging, ... | 2015 | 26408140 |
| effects of two application methods of plantaricin bm-1 on control of listeria monocytogenes and background spoilage bacteria in sliced vacuum-packaged cooked ham stored at 4°c. | two application methods were used to investigate the effect of plantaricin bm-1 on the control of listeria monocytogenes and background spoilage bacteria in sliced vacuum-packaged cooked ham without the addition of any chemical preservatives, including sodium nitrite, during 35 days of storage at 4°c. regardless of the application method, plantaricin bm-1 treatment (320, 640, or 1,280 arbitrary units [au]/g of sliced cooked ham) significantly (p < 0.05) reduced the survival of l. monocytogenes ( ... | 2015 | 26408132 |
| prevalence and distribution of listeria monocytogenes inla alleles prone to phase variation and inla alleles with premature stop codon mutations among human, food, animal, and environmental isolates. | in listeria monocytogenes, 18 mutations leading to premature stop codons (pmscs) in the virulence gene inla have been identified to date. while most of these mutations represent nucleotide substitutions, a frameshift deletion in a 5' seven-adenine homopolymeric tract (ht) in inla has also been reported. this ht may play a role in phase variation and was first identified among l. monocytogenes lineage ii ribotype dup-1039c isolates. in order to better understand the distribution of different inla ... | 2015 | 26407886 |
| prevalence and virulence characterization of listeria monocytogenes in chilled pork in zhejiang province, china. | listeria monocytogenes is an important foodborne pathogen that can grow in refrigeration temperature and causes severe human infections. the aims of this work were to estimate the prevalence of l. monocytogenes in chilled pork in zhejiang, china and to examine the virulence features of the isolates. of 331 meat samples, 196 were positive for listeria spp., with l. innocua accounting for 54.4%, l. monocytogenes for 11.5%, and l. welshimeri for 4.2%. the most prevalent l. monocytogenes serotype wa ... | 2016 | 26393675 |
| a physical/psychological and biological stress combine to enhance endoplasmic reticulum stress. | the generation of an immune response against infectious and other foreign agents is substantially modified by allostatic load, which is increased with chemical, physical and/or psychological stressors. the physical/psychological stress from cold-restraint (cr) inhibits host defense against listeria monocytogenes (lm), due to early effects of the catecholamine norepinephrine (ne) from sympathetic nerves on β1-adrenoceptors (β1ar) of immune cells. although cr activates innate immunity within 2h, h ... | 2015 | 26391182 |
| using uvc light-emitting diodes at wavelengths of 266 to 279 nanometers to inactivate foodborne pathogens and pasteurize sliced cheese. | uvc light is a widely used sterilization technology. however, uv lamps have several limitations, including low activity at refrigeration temperatures, a long warm-up time, and risk of mercury exposure. uv-type lamps only emit light at 254 nm, so as an alternative, uv light-emitting diodes (uv-leds) which can produce the desired wavelengths have been developed. in this study, we validated the inactivation efficacy of uv-leds by wavelength and compared the results to those of conventional uv lamps ... | 2015 | 26386061 |
| propofol increases host susceptibility to microbial infection by reducing subpopulations of mature immune effector cells at sites of infection. | anesthetics are known to modulate host immune responses, but separating the variables of surgery from anesthesia when analyzing hospital acquired infections is often difficult. here, the bacterial pathogen listeria monocytogenes (lm) was used to assess the impact of the common anesthetic propofol on host susceptibility to infection. brief sedation of mice with physiologically relevant concentrations of propofol increased bacterial burdens in target organs by more than 10,000-fold relative to inf ... | 2015 | 26381144 |
| combined effect of ultrasound and essential oils to reduce listeria monocytogenes on fresh produce. | salads prepared from contaminated fresh produce have a high risk of causing food-borne illnesses. essential oils obtained from plants have antimicrobial activity and may provide a natural approach to reduce the pathogens on fresh produce. additionally, ultrasound treatments have been shown to reduce the microbial counts on different foods. the objective of this study was to investigate the antimicrobial activities of cinnamon and lemon essential oils in vitro and in food applications. mixtures o ... | 2016 | 26377335 |
| nosocomial neonatal listeria monocytogenes transmission by stethoscope. | | 2015 | 26376314 |
| an unusual presentation of paediatric listeria meningitis with selective spinal grey matter involvement and acute demyelinating polyneuropathy. | paediatric listeria meningitis is rare, especially in immuno-competent children, but associated with significant mortality and morbidity and frequent complications. | 2016 | 26371981 |
| domain function dissection and catalytic properties of listeria monocytogenes p60 protein with bacteriolytic activity. | the major extracellular protein p60 of listeria monocytogenes (lm-p60) is an autolysin that can hydrolyze the peptidoglycan of bacterial cell wall and has been shown to be required for l. monocytogenes virulence. the predicted three-dimensional structure of lm-p60 showed that lm-p60 could be split into two independent structural domains at the amino acid residue 270. conserved motif analysis showed that v30, d207, s395, and h444 are the key amino acid residues of the corresponding motifs. howeve ... | 2015 | 26363556 |
| cutting edge: the orphan nuclear receptor nr4a1 regulates cd8+ t cell expansion and effector function through direct repression of irf4. | the transcription factor ifn regulatory factor (irf)4 was shown to play a crucial role in the protective cd8(+) t cell response; however, regulation of irf4 expression in cd8(+) t cells remains unclear. in this article, we report a critical role for nr4a1 in regulating the expansion, differentiation, and function of cd8(+) t cells through direct transcriptional repression of irf4. without nr4a1, the regulation of irf4 is lost, driving an increase in irf4 expression and, in turn, resulting in a f ... | 2015 | 26363057 |
| meta-analysis of the effects of sanitizing treatments on salmonella, escherichia coli o157:h7, and listeria monocytogenes inactivation in fresh produce. | the aim of this study was to perform a meta-analysis of the effects of sanitizing treatments of fresh produce on salmonella spp., escherichia coli o157:h7, and listeria monocytogenes. from 55 primary studies found to report on such effects, 40 were selected based on specific criteria, leading to more than 1,000 data on mean log reductions of these three bacterial pathogens impairing the safety of fresh produce. data were partitioned to build three meta-analytical models that could allow the asse ... | 2015 | 26362982 |
| biofilm-forming abilities of listeria monocytogenes serotypes isolated from different sources. | a total of 98 previously characterized and serotyped l. monocytogenes strains, comprising 32 of 1/2a; 20 of 1/2b and 46 of 4b serotype, from clinical and food sources were studied for their capability to form a biofilm. the microtiter plate assay revealed 62 (63.26%) strains as weak, 27 (27.55%) strains as moderate, and 9 (9.18%) strains as strong biofilm formers. among the strong biofilm formers, 6 strains were of serotype 1/2a and 3 strains were of serotype 1/2b. none of the strain from 4b ser ... | 2015 | 26360831 |
| listeria meningitis complicating a patient with ulcerative colitis on concomitant infliximab and hydrocortisone. | infliximab, a monoclonal antibody directed against tumour necrosis factor, is an effective therapy for moderate-to-severe ulcerative colitis and crohn's disease. uncommonly, serious opportunistic infections have occurred in patients after infliximab administration. here, we describe meningitis caused by listeria monocytogenes developing in a 37-year-old man with ulcerative colitis refractory to intravenous corticosteroids 10 days after receiving his first infusion of infliximab. with the increas ... | 2016 | 26358724 |
| mining of novel species-specific primers for pcr detection of listeria monocytogenes based on genomic approach. | listeria monocytogenes in contaminated food is considered as a serious health threat for consumers due to its high mortality rate. the objective of this study was to obtain novel species-specific target-genes and primers for the molecular detection of l. monocytogenes using a comparative genomic approach. by comparative analysis of l. monocytogenes and non-l. monocytogenes genome sequences in the genbank database with blast program, 26 specific target sequences were used as candidates and the pr ... | 2015 | 26354019 |
| combination of zinc and all-trans retinoic acid promotes protection against listeria monocytogenes infection. | zinc (zn) is the second most abundant transition metal after iron. it plays a vital role in living organisms and affects multiple aspects of the immune system. all-trans retinoic acid (atra) is an isomeric form of the vitamin a or retinol. it possesses the greatest biological activity of vitamin a. vitamin a and related retinoids influence many aspects of immunity. in this study, we demonstrated that treatment with a combination of zn and atra contributes to host resistance against infection by ... | 2015 | 26351852 |
| fisetin protects against listeria monocytogenes infection by reducing the production of listeriolysin o. | | 2016 | 26347572 |
| polymer-based microfluidic chip for rapid and efficient immunomagnetic capture and release of listeria monocytogenes. | infections caused by foodborne pathogens such as listeria monocytogenes pose a threat to public health while timely detection is challenging due to pathogen low numbers. the development of robust and efficient sample preparation techniques is crucial to improve detection sensitivity and workflow. immunomagnetic separation using magnetic nanoparticles (mnps) is attractive, as it can efficiently capture target cells. for food safety applications, a platform is needed to rapidly process large sampl ... | 2015 | 26346021 |
| production of antilisterial bacteriocins from lactic acid bacteria in dairy-based media: a comparative study. | one hundred and eight strains of lactic acid bacteria (lab) were screened for bacteriocin production by the modified deferred antagonism and agar well diffusion methods. when the modified deferred antagonism method was employed, 82 lab strains showed inhibitory action against listeria monocytogenes v7 ½a, whereas 26 lab strains expressed no inhibition. only 12 lab strains exhibited inhibitory activity when the agar well diffusion method was used, 11 of which had been previously recognized as bac ... | 2015 | 26341641 |
| estimation of listeria monocytogenes survival during thermoultrasonic treatments in non-isothermal conditions: effect of ultrasound on temperature and survival profiles. | estimation of listeria monocytogenes survival during thermoultrasonic treatments in non-isothermal conditions was determined considering an increment from 45 to 70 °c, assessing the adequacy of predictions through experimental data obtained in laboratory media model systems. in order to characterize the sonication effect on the survival pattern, observed behavior was compared to that obtained when only thermal treatment was applied. a noticeable impact on l. monocytogenes survival in non-isother ... | 2015 | 26338125 |
| bacteriophage cocktail significantly reduces or eliminates listeria monocytogenes contamination on lettuce, apples, cheese, smoked salmon and frozen foods. | listshield™, a commercially available bacteriophage cocktail that specifically targets listeria monocytogenes, was evaluated as a bio-control agent for l. monocytogenes in various ready-to-eat foods. listshield™ treatment of experimentally contaminated lettuce, cheese, smoked salmon, and frozen entrèes significantly reduced (p < 0.05) l. monocytogenes contamination by 91% (1.1 log), 82% (0.7 log), 90% (1.0 log), and 99% (2.2 log), respectively. listshield™ application, alone or combined with an ... | 2015 | 26338115 |
| erratum: increased spread and replication efficiency of listeria monocytogenes in organotypic brain-slices is related to multilocus variable number of tandem repeat analysis (mlva) complex. | | 2015 | 26335249 |
| antimicrobial poly(lactic acid)-based nanofibres developed by solution blow spinning. | the present study reports on the development of hybrid poly(lactic acid) (pla) fibres loaded with highly crystalline bacterial cellulose nanowhiskers (bcnw) by the novel solution blow spinning method. furthermore, fibres with antimicrobial properties were generated by incorporating carvacrol and thc as antimicrobial agents and the biocide effect against listeria monocytogenes was studied. initially, pla blow spun fibres containing bcnw were optimized in terms of morphology and thermal properties ... | 2015 | 26328414 |
| behaviour of listeria monocytogenes in artisanal raw milk pecorino umbro cheese: a microbiological challenge test. | in the present study, a microbiological challenge test in artificially contaminated raw milk pecorino umbro cheese during cheese-making was carried out. raw ewe milk was contaminated by a suspension of particular listeria monocytogenes strains. the number of l. monocytogenes and l. monocytogenes dynamic growth were evaluated during cheese-making and storage. a significant decrease of the viable count of l. monocytogenes was observed during ripening and l. monocytogenes viable count was below the ... | 2015 | 27800412 |
| diversity of listeria monocytogenes within a u.s. dairy herd, 2004-2010. | listeria monocytogenes, the causative agent of listeriosis, is frequently isolated from the environment. dairy cows and dairy farm environments are reservoirs of this pathogen, where fecal shedding contributes to its environmental dispersal and contamination of milk, dairy products, and meat. the molecular diversity of 40 l. monocytogenes isolates representing 3 serogroups (1/2a, 1/2b, and 4b) collected between 2004 and 2010 from the feces of dairy cattle on a single dairy farm was assessed usin ... | 2015 | 26325149 |