| hlya gene-based sensitive detection of listeria monocytogenes using a novel cantilever sensor. | piezoelectric cantilever sensors are shown to exhibit sensitive and selective detection based on an identifying gene from genomic extract at ~10(2)-10(3) cells of foodborne pathogen, listeria monocytogenes (lm). the study consists of two parts: tests with synthetic genes and experiments starting with whole lm cells. a probe designed for the virulence hemolysin gene, hlya, was immobilized on the gold-coated sensor, and hybridization detection of a synthetic target (based on hlya) is shown to span ... | 2013 | 23418904 |
| comparative evaluation on fatty acid and matricaria recutita essential oil incorporated into casein-based film. | sodium caseinate composite films containing lipids-oleic acid (oa), stearic acid (sa), or matricaria recutita essential oil (meo) - were prepared through emulsification and their physical, thermal, mechanical, and barrier properties were evaluated and compared. furthermore, their antimicrobial effectiveness against listeria monocytogenes, staphylococcus aureus, and escherichia coli was studied. emulsified films were softer, less rigid, and more stretchable than pure films. the films' water vapor ... | 2013 | 23415659 |
| physicochemical quality and chemical safety of chlorine as a reconditioning agent and wash water disinfectant for fresh-cut lettuce washing. | chlorine was assessed as a reconditioning agent and wash water disinfectant in the fresh-cut produce industry. artificial fresh-cut lettuce wash water, made from butterhead lettuce, was used for the experiments. in the reconditioning experiments, chlorine was added to artificial wash water inoculated with escherichia coli o157 (6 log cfu/ml). regression models were constructed based on the inactivation data and validated in actual wash water from leafy vegetable processing companies. the model t ... | 2013 | 23396332 |
| novel antibody/gold nanoparticle/magnetic nanoparticle nanocomposites for immunomagnetic separation and rapid colorimetric detection of staphylococcus aureus in milk. | we demonstrated the new antibody/gold nanoparticle/magnetic nanoparticle nanocomposites (antibody/aunp/mnps) and their application in the detection of the foodborne pathogen, staphylococcus aureus (s. aureus), in milk. the nanocomposites were synthesized by coating the mnps with bovine serum albumin (bsa) then adsorbing the aunps and anti-s. aureus antibodies on their surface. using the completed immunomagnetic nanostructures, s. aureus inoculated in the milk sample was captured and isolated fro ... | 2013 | 23370174 |
| cell-mediated killing of listeria monocytogenes by leucocin c producing escherichia coli. | listeria monocytogenes is a foodborne pathogen causing listeriosis. listeria in foods can be inhibited with bacteriocins or bacteriocin producing cultures. the aim of this study was to enhance the killing of l. monocytogenes by binding bacteriocin producing escherichia coli cells to listeria cells. antilisterial e. coli was obtained by transferring leucocin c production from leuconostoc carnosum 4010. for binding of e. coli cells to listeria cells, the listeria phage endolysin plyp35 cell wall b ... | 2013 | 23348073 |
| antioxidant and antimicrobial properties of monofloral bee pollen. | the main aim of this study was to determine antioxidant properties and antibacterial activity of monofloral bee pollen samples to pathogenic bacteria. these samples were collected in different localities in slovakia. the antioxidant properties of examined plant species were different and decreasing in the following order: brassica napus subsp. napus l > papaver somniferum l. > helianthus annuus l. the antimicrobial effect of the bee product samples were tested by using the agar well diffusion me ... | 2013 | 23305281 |
| [role of pathogen surveillance and subtyping in outbreak recognition of food-borne bacterial infections. microbiological perspective - aims, methods and prospects of pathogen subtyping]. | the recognition of infection clusters via determination of clonal relationships between pathogen isolates represents the major aim of pathogen subtyping during outbreaks. in addition, a continuing and comprehensive subtyping of pathogen isolates is a prerequisite for early recognition of changes within pathogen populations, especially of new pathogen types and variants. here, in an exemplary manner, we outline the current practice in germany for three important agents of food-borne infections, s ... | 2013 | 23275955 |
| [the need for systematic molecular subtyping surveillance to timely detect food-borne, bacterial outbreaks]. | timely outbreak detection is a major objective of the surveillance of food-borne infections. in this regard molecular subtyping is a very useful tool for several bacterial pathogens, e.g. enterohemorrhagic e. coli (ehec), listeria monocytogenes and various serotypes of non-typhoidal salmonella. the basis is the characterization of patient isolates by molecular methods, preferably at the clonal level. the goal is to find groups of identical isolates which may indicate that they share a common ori ... | 2013 | 23275954 |
| combined effect of sodium nitrite with high-pressure treatments on the inactivation of escherichia coli bw25113 and listeria monocytogenes nctc 11994. | the bactericidal effect of acidified sodium nitrite alone or when combined with high hydrostatic pressure (hhp) treatment was examined with escherichia coli bw25113 and listeria monocytogenes nctc 11994. a powerful synergistic effect of hhp plus nitrite was observed at ph 4·0, but not at higher ph values. escherichia coli hmpa and norv mutants lacking defences against nitrosative stress were more sensitive to pressure combined with acidified sodium nitrite than the wild-type strain, suggesting a ... | 2013 | 23206192 |
| effects of combined treatments of electron-beam irradiation and addition of leek (allium tuberosum) extract on reduction of pathogens in pork jerky. | this study investigated the combined effect of electron-beam irradiation and addition of leek (allium tuberosum r.) extract on pork jerky inoculated with selected foodborne pathogens. prepared pork jerky samples (control and samples with 1.0% leek extract) were inoculated with pathogens and subsequently irradiated at 0, 0.5, 1, 2, 3, and 4 kgy doses. in comparison with the control, samples with 1.0% leek extract showed significant reduction in the numbers of escherichia coli, listeria monocytoge ... | 2012 | 23199493 |
| functional characterization of three mouse formyl peptide receptors. | the evolutionary relationship and functional correlation between human formyl peptide receptors (fprs) and their mouse counterparts remain incompletely understood. we examined three members of the mouse formyl peptide receptor subfamily (mfprs) and found that they differ in agonist preference and cellular distributions. when stably expressed in transfected rat basophilic leukemia (rbl-2h3) cells, mfpr1 was readily activated by n-formylated peptides derived from listeria monocytogenes (fmivtlf), ... | 2013 | 23160941 |
| modified-atmosphere packaging of hen table eggs: effects on pathogen and spoilage bacteria. | as part of a more comprehensive research activity on the use of modified-atmosphere packaging for the improvement of quality and functional properties of table eggs, the effects of air, 100% co(2), and 100% o(2) packaging were also evaluated on the survival of experimentally inoculated pathogen bacteria (salmonella enteritidis, escherichia coli, and listeria monocytogenes) as well as on spoilage bacteria (total aerobic mesophilic bacteria) on table eggs during 30 d of storage at 4, 25, and 37°c ... | 2012 | 23155038 |
| landscape and meteorological factors affecting prevalence of three food-borne pathogens in fruit and vegetable farms. | produce-related outbreaks have been traced back to the preharvest environment. a longitudinal study was conducted on five farms in new york state to characterize the prevalence, persistence, and diversity of food-borne pathogens in fresh produce fields and to determine landscape and meteorological factors that predict their presence. produce fields were sampled four times per year for 2 years. a total of 588 samples were analyzed for listeria monocytogenes, salmonella, and shiga toxin-producing ... | 2013 | 23144137 |
| efficient separation and sensitive detection of listeria monocytogenes using an impedance immunosensor based on magnetic nanoparticles, a microfluidic chip, and an interdigitated microelectrode. | listeria monocytogenes continues to be a major foodborne pathogen that causes food poisoning, and sometimes death, among immunosuppressed people and abortion among pregnant women. in this study, magnetic nanoparticles with a diameter of 30 nm were functionalized with anti-l. monocytogenes antibodies via biotin-streptavidin bonds to become immunomagnetic nanoparticles (imnps) to capture l. monocytogenes in a sample during a 2-h immunoreaction. a magnetic separator was used to collect and hold the ... | 2012 | 23127703 |
| a novel synthetic peptide from a tomato defensin exhibits antibacterial activities against helicobacter pylori. | defensins are a class of cysteine-rich proteins, which exert broad spectrum antimicrobial activity. in this work, we used a bioinformatic approach to identify putative defensins in the tomato genome. fifteen proteins had a mature peptide that includes the well-conserved tetradisulfide array. we selected a representative member of the tomato defensin family; we chemically synthesized its γ-motif and tested its antimicrobial activity. here, we demonstrate that the synthetic peptide exhibits potent ... | 2012 | 23124812 |
| behaviour of co-inoculated pathogenic and spoilage bacteria on poultry following several decontamination treatments. | the potential of chemical decontaminants to cause harmful effects on human health is among the causes of the rejection of antimicrobial treatments for removing surface contamination from poultry carcasses in the european union. this study was undertaken to determine whether decontaminants might give a competitive advantage to pathogenic bacteria on poultry and involve a potential risk to consumer. a total of 144 chicken legs were co-inoculated with similar concentrations of pathogenic bacteria ( ... | 2012 | 23072701 |
| new insights in mechanisms of bacterial inactivation by carvacrol. | to study the mechanism of bacterial inactivation by carvacrol and the influence of genetic and environmental factors in its antimicrobial activity. | 2013 | 23035895 |
| development of bacteria identification array to detect lactobacilli in thai fermented sausage. | to improve the quality and safety of food products, there is a need in the food industry for a reliable method for simultaneously monitoring multiple bacterial strains. microarray technology is a high-throughput screening approach that can provide an alternative for bacteria detection. a total of 164 bacteria-specific probes were designed from 16s rrna gene sequences to target 12 bacteria species, including lactic acid bacteria and selected food pathogens. after fabrication onto aminosilane-coat ... | 2012 | 23022427 |
| [zoonotic potential of raw cow's milk in the czech republic]. | to assess current microbiological risks of raw cow's milk. the presented work reports the prevalence of selected bacteria on czech dairy farms in 2010 and compares the results with a similar study in 2002. | 2012 | 22997775 |
| glutamate decarboxylase-dependent acid resistance in orally acquired bacteria: function, distribution and biomedical implications of the gadbc operon. | for successful colonization of the mammalian host, orally acquired bacteria must overcome the extreme acidic stress (ph < 2.5) encountered during transit through the host stomach. the glutamate-dependent acid resistance (gdar) system is by far the most potent acid resistance system in commensal and pathogenic escherichia coli, shigella flexneri, listeria monocytogenes and lactococcus lactis. gdar requires the activity of glutamate decarboxylase (gadb), an intracellular plp-dependent enzyme which ... | 2012 | 22995042 |
| foodborne pathogens in in-line milk filters and associated on-farm risk factors in dairy farms authorized to produce and sell raw milk in northern italy. | all dairy farms authorized to produce and sell raw milk in a province of northern italy were investigated to determine the presence of campylobacter spp., verocytotoxin-producing escherichia coli (vtec), listeria monocytogenes, and salmonella spp. in in-line milk filters and to assess their association with suspected risk factors on farms. a logistic regression model was used to analyze data collected describing the characteristics and management practices of 27 farms and the microbiological sta ... | 2012 | 22980010 |
| effects of pressurization on some contamination flora in beef pate. | pate meat is one of the food products at the top of the high-risk category since it is an undercooked product, which does not undergo any treatment to ensure its safety before consumption. in this study, pressurization, a nonthermal technology, was administered to the product to enhance its microbial safety for the 1st time. after being cooked, ground, and mixed with broth and spices, the pate meat was divided into 3 batches. their additional fat contents were adjusted to 9.11%, 25.00%, and 35.0 ... | 2012 | 22924817 |
| the timing of ifnβ production affects early innate responses to listeria monocytogenes and determines the overall outcome of lethal infection. | dendritic cells (dcs) and natural killer (nk) cells are essential components of the innate immunity and play a crucial role in the first phase of host defense against infections and tumors. listeria monocytogenes (lm) is an intracellular pathogen that colonizes the cytosol of eukaryotic cells. recent findings have shown lm specifically in splenic cd8a(+) dcs shortly after intravenous infection. we examined gene expression profiles of mouse dcs exposed to lm to elucidate the molecular mechanisms ... | 2012 | 22912878 |
| effects of holdfast of laminaria japonica on listeria invasion on enterocyte-like caco-2 cells and no production of macrophage raw 264.7 cells. | listeria monocytogenes (lm) causes food poisoning in humans mainly through consumption of ready-to-eat foods. immunocompromised persons are at the highest risk for infection. we investigated effects of crude soluble polysaccharides (sps) and ethanolic extract (ee) fractions of frond (kombu) and holdfast (ganiashi) parts of laminaria japonica on lm invasion into human enterocyte-like caco-2 cells and immune and/or inflammatory reactions of murine macrophage raw 264.7 cells. recovery and viscosity ... | 2012 | 22893519 |
| listeria monocytogenes peritonitis: presentation, clinical features, treatment, and outcome. | spontaneous bacterial peritonitis (sbp) is a serious complication in cirrhotic patients. gram (-) (e. coli, klebsiella pneumoniae), and gram (+) (streptococci, staphylococci) bacteria are most frequently cultured from patients'ascites. listeria monocytogenes (lm) is scarcely reported as a causative agent. | 2012 | 22834987 |
| the activity of ferulic and gallic acids in biofilm prevention and control of pathogenic bacteria. | the activity of two phenolic acids, gallic acid (ga) and ferulic acid (fa) at 1000 μg ml(-1), was evaluated on the prevention and control of biofilms formed by escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and listeria monocytogenes. in addition, the effect of the two phenolic acids was tested on planktonic cell susceptibility, bacterial motility and adhesion. biofilm prevention and control were tested using a microtiter plate assay and the effect of the phenolic acids was asse ... | 2012 | 22823343 |
| years of potential life lost for six major enteric pathogens, germany, 2004-2008. | in industrialized countries, acute infectious enteric diseases are usually mild, but they can also cause death. they do so, however, at different ages. using 2004-2008 german notification data, we computed and compared crude and premature mortality [three different measures of years of potential life lost (ypll)] of illnesses caused by campylobacter spp., listeria monocytogenes, norovirus, rotavirus, non-typhoidal salmonella spp., and shiga toxin-producing e. coli (stec). among ~1.5 million noti ... | 2013 | 22813426 |
| [mri findings of neonatal purulent meningitis caused by different pathogenic bacteria]. | to study the image characteristics of neonatal purulent meningitis caused by different pathogenic bacteria, using conventional magnetic resonance imaging (mri). | 2012 | 22809598 |
| pulsed light treatment for the inactivation of selected pathogens and the shelf-life extension of beef and tuna carpaccio. | the efficacy of pulsed light to improve the safety of carpaccio has been investigated. beef and tuna slices were superficially inoculated with approximately 3 log cfu/cm2 of listeria monocytogenes, escherichia coli, salmonella typhimurium and vibrio parahaemolyticus. fluences of 0.7, 2.1, 4.2, 8.4 and 11.9 j/cm2 were assayed. colour, sensory and shelf-life studies were carried out. treatments at 8.4 and 11.9 j/cm2 inactivated the selected pathogens approximately by 1 log cfu/cm2, although they m ... | 2012 | 22795799 |
| nlrp6 negatively regulates innate immunity and host defence against bacterial pathogens. | members of the intracellular nucleotide-binding and oligomerization domain (nod)-like receptor (nlr) family contribute to immune responses through activation of nuclear factor-κb (nf-κb), type i interferon and inflammasome signalling. mice lacking the nlr family member nlrp6 were recently shown to be susceptible to colitis and colorectal tumorigenesis, but the role of nlrp6 in microbial infections and the nature of the inflammatory signalling pathways regulated by nlrp6 remain unclear. here we s ... | 2012 | 22763455 |
| construction of recombinant e. coli nissle 1917 (ecn) strains for the expression and secretion of defensins. | the probiotic escherichia coli strain nissle 1917 (ecn) is one of the few probiotics licensed as a medication in several countries. best documented is its effectiveness in keeping patients suffering from ulcerative colitis (uc) in remission. this might be due to its ability to induce the production of human β-defensin 2 (hbd2) in a flagellin-dependent way in intestinal epithelial cells. in contrast to ulcerative colitis, for crohn's disease (cd) convincing evidence is lacking that ecn might be c ... | 2012 | 22748509 |
| identification of volatile components of liverwort (porella cordaeana) extracts using gc/ms-spme and their antimicrobial activity. | chemical constituents of liverwort (porella cordaeana) extracts have been identified using solid-phase microextraction-gas chromatography mass spectrometry (spme-gc/ms). the methanol, ethanol and ethyl acetate extracts were rich in terpenoids such as sesquiterpene hydrocarbons (53.12%, 51.68%, 23.16%), and monoterpene hydrocarbons (22.83%, 18.90%, 23.36%), respectively. the dominant compounds in the extracts were β-phellandrene (15.54%, 13.66%, 12.10%) and β-caryophyllene (10.72%, 8.29%, 7.79%, ... | 2012 | 22728370 |
| [accelerated method for microbiological control of the safety of foodstuffs]. | the action and devices of rapid singlepath (merck kgaa) tests are considered. the singlepath tests are used for the accelerated detection of pathogenic bacteria (salmonella spp., campylobacter spp., listeria monocytogenes, e. coli o157) in the raw products and foodstuff. the tests as compared to conventional methods showed a high similarity of results in identifying salmonella in raw meat material. the immunochromatographic test requires no equipment and it is characterized by simple sample prep ... | 2012 | 22712333 |
| microbiological profile of milk: impact of household practices. | milk is susceptible to contamination by many microorganisms including microbial pathogens responsible for causing diseases. various processes including pasteurization, boiling or storage under refrigerated conditions are undertaken to minimize the microbial contamination of milk. | 2012 | 22684182 |
| antimicrobial food packaging film based on the release of lae from evoh. | the aim of this work was to develop antimicrobial films for active packaging applications containing the natural antimicrobial compound lae (lauramide arginine ethyl ester) in evoh copolymers with different mol % ethylene contents (i.e. evoh-29 and evoh-44). evoh-29 and evoh-44 films were made by casting and incorporating 0.25%, 1%, 5%, and 10% lae in the film forming solution (w/w with respect to polymer weight). previously, the minimum inhibitory concentration (mic) and the minimum bactericida ... | 2012 | 22640726 |
| development of antimicrobial films for microbiological control of packaged salad. | the aim of the present work was to characterize the antimicrobial efficiency of films consisting of pp/evoh structures with oregano essential oil and citral. both substances are known for their antimicrobial activity based on their interaction with the cell membrane. the films developed were used to pack minimally processed salads, combining modified atmosphere technology to extend shelf-life and active packaging technology to reduce possible microbiological risks. the antimicrobial activity of ... | 2012 | 22633535 |
| estimates of enteric illness attributable to contact with animals and their environments in the united states. | contact with animals and their environment is an important, and often preventable, route of transmission for enteric pathogens. this study estimated the annual burden of illness attributable to animal contact for 7 groups of pathogens: campylobacter species, cryptosporidium species, shiga toxin-producing escherichia coli (stec) o157, stec non-o157, listeria monocytogenes, nontyphoidal salmonella species, and yersinia enterocolitica. | 2012 | 22572672 |
| high-intensity 405 nm light inactivation of listeria monocytogenes. | the antimicrobial properties of light is an area of increasing interest. this study investigates the sensitivity of the significant foodborne pathogen listeria monocytogenes to selected wavelengths of visible light. results demonstrate that exposure to wavelength region 400-450 nm, at sufficiently high dose levels (750 j cm(-2)), induced complete inactivation of a 5 log(10) population. exposure to wavelengths longer than 450 nm did not cause significant inactivation. analysis of 10 nm bandwidths ... | 2012 | 22582879 |
| multidrug-resistant bacterial infection in solid organ transplant recipients. | the most frequent complication from infection after solid organ transplantation is bacterial infection. this complication is more frequent in organ transplantation involving the abdominal cavity, such as liver or pancreas transplantation, and less frequent in heart transplant recipients. the sources, clinical characteristics, antibiotic resistance and clinical outcomes vary according to the time of onset after transplantation. most bacterial infections during the first month post-transplantation ... | 2012 | 22542034 |
| study on carvacrol and cinnamaldehyde polymeric films: mechanical properties, release kinetics and antibacterial and antibiofilm activities. | polyethylene-co-vinylacetate (eva) films with different concentrations (3.5 wt% and 7 wt%) of essential oil constituents, carvacrol or cinnamaldehyde, were prepared and characterized by mechanical, antibacterial and antibiofilm properties. the incorporation of the compounds into copolymer films affected their elastic modulus, tensile stress and elongation at break. carvacrol and cinnamaldehyde act as plasticizers which reduce the intermolecular forces of polymer chains, thus improving the flexib ... | 2012 | 22555914 |
| factors affecting quality and safety of fresh-cut produce. | the quality of fresh-cut fruit and vegetable products includes a combination of attributes, such as appearance, texture, and flavor, as well as nutritional and safety aspects that determine their value to the consumer. nutritionally, fruit and vegetables represent a good source of vitamins, minerals, and dietary fiber, and fresh-cut produce satisfies consumer demand for freshly prepared, convenient, healthy food. however, fresh-cut produce deteriorates faster than corresponding intact produce, a ... | 2012 | 22530712 |
| variability of rna quality extracted from biofilms of foodborne pathogens using different kits impacts mrna quantification by qpcr. | the biofilm formation by foodborne pathogens is known to increase the problem related with surface disinfection procedure in the food processing environment and consequent transmission of these pathogens into the population. messenger rna has been increasingly used to understand the action and the consequences of disinfectants in the virulence on such biofilms. rna quality is an important requirement for any rna-based analysis since the quality can impair the mrna quantification. therefore, we e ... | 2012 | 22526569 |
| bactericidal antibiotics do not appear to cause oxidative stress in listeria monocytogenes. | oxidative stress can be an important contributor to the lethal effect of bactericidal antibiotics in some bacteria, such as escherichia coli and staphylococcus aureus. thus, despite the different target-specific actions of bactericidal antibiotics, they have a common mechanism leading to bacterial self-destruction by internal production of hydroxyl radicals. the purpose of the present study was to determine if a similar mechanism is involved in antibiotic killing of the infectious human pathogen ... | 2012 | 22504823 |
| insight into the prevalence and distribution of microbial contamination to evaluate water management in the fresh produce processing industry. | this study provided insight into the degree of microbial contamination in the processing chain of prepacked (bagged) lettuce in two belgian fresh-cut produce processing companies. the pathogens salmonella and listeria monocytogenes were not detected. total psychrotrophic aerobic bacterial counts (tpacs) in water samples, fresh produce, and environmental samples suggested that the tpac is not a good indicator of overall quality and best manufacturing practices during production and processing. be ... | 2012 | 22488054 |
| microbiological surveillance of a bovine raw milk farm through multiplex real-time pcr. | raw milk is increasingly appreciated by consumers but can be contaminated by a variety of zoonotic pathogens. therefore, preventive measures, such as on-farm hazard analysis critical control point (haccp) programs, must be applied to protect consumers. the aim of the present study was the comparison of a multiplex real-time polymerase chain reaction (pcr) assay with a culture-based approach in an on-farm quality assurance program for the detection of escherichia coli o157, salmonella spp., and l ... | 2012 | 22471929 |
| [prokaryotic expression and identification of hpv16 e7 protein]. | hpv16 e7 fusion protein was expressed in e. coli bl21, and its applied value for hpv was evaluated. hpv16 e7 gene was amplified by pcr, and cloned into prokaryotic expression vector pgex6p-1. the recombinant plasmid was transformed into e. coli bl21, and hpv16 e7 fusion was expressed through iptg induction. the expressed product was analyzed by sds-page and western blot, subsequently purified according to glutathione sepharose 4b purification procedure. an indirect elisa with the purified fusion ... | 2012 | 22416350 |
| nexilin is a dynamic component of listeria monocytogenes and enteropathogenic escherichia coli actin-rich structures. | the bacterial pathogens listeria monocytogenes and enteropathogenic escherichia coli (epec) generate motile actin-rich structures (comet tails and pedestals) as part of their infectious processes. nexilin, an actin-associated protein and a component of focal adhesions, has been suggested to be involved in actin-based motility. to determine whether nexilin is commandeered during l. monocytogenes and epec infections, we infected cultured cells and found that nexilin is crucial for l. monocytogenes ... | 2012 | 22381134 |
| changing epidemiology of bacteremia in infants aged 1 week to 3 months. | bacteremia in young infants has remained an important ongoing concern for decades. despite changes in prenatal screening and infant immunizations, the current epidemiology of this problem has received little attention. | 2012 | 22371459 |
| a selected probiotic strain of lactobacillus fermentum cm33 isolated from breast-fed infants as a potential source of β-galactosidase for prebiotic oligosaccharide synthesis. | lactic acid bacteria from healthy breast-fed infants were isolated and screened for β-galactosidase production in mrs broth. among 49 isolates that exhibited the yellow clear zone on mrs agar supplemented with bromocresol blue, the isolate cm33 was selected as being the highest β-galactosidase producer and was identified as lactobacillus fermentum based on its morphological characteristics and 16s rdna nucleotide sequence. l. fermentum cm33 exhibited a good survival rate under the simulated stom ... | 2012 | 22367946 |
| recombinant expression of a putative amidase cloned from the genome of listeria monocytogenes that lyses the bacterium and its monolayer in conjunction with a protease. | listeria monocytogenes is a gram-positive, non-spore forming, catalase-positive rod that is a major bacterial food-borne disease agent associated with uncooked meats, including poultry, uncooked vegetables, soft cheeses, and unpasteurized milk. the bacterium may be carried by animals without signs of disease, can replicate at refrigeration temperatures, and is frequently associated with biofilms. there is a need to discover innovative pathogen intervention technologies for this bacterium. conseq ... | 2012 | 26781731 |
| fructooligosaccharides metabolism and effect on bacteriocin production in lactobacillus strains isolated from ensiled corn and molasses. | fructo- (fos) and galacto-oligosaccharides have been used to promote the growth of probiotics, mainly those from lactobacillus genus. however, only few reports have evaluated the effect of prebiotics on bacteriocins activity and production. in this work, we characterized the effect of fos supplementation on the growth, lactic and acetic acids production, and antimicrobial activity of crude extracts obtained from lactobacillus strains isolated from ensiled corn and molasses. seven out of 28 isola ... | 2012 | 22342961 |
| development of a paper-based analytical device for colorimetric detection of select foodborne pathogens. | foodborne pathogens are a major public health threat and financial burden for the food industry, individuals, and society, with an estimated 76 million cases of food-related illness occurring in the united states alone each year. three of the most important causative bacterial agents of foodborne diseases are pathogenic strains of escherichia coli , salmonella spp., and listeria monocytogenes , due to the severity and frequency of illness and disproportionally high number of fatalities. their co ... | 2012 | 22320200 |
| cloning and heterologous expression of a bacteriocin sakacin p from lactobacillus sakei in escherichia coli. | sakacin p, a bacteriocin from lactobacillus sakei, shows strong activity against food-borne pathogens such as listeria monocytogenes. in l. sakei, the structural gene (sppa) encoding sakacin p is controlled by a strict regulatory mechanism, and the quantity of secreted sakacin p is limited. in this study, the sppa gene was synthesized by splicing overlap extension pcr and cloned into escherichia coli. after the induction with isopropyl-β-d-thiogalactopyranoside, the recombinant sakacin p was suc ... | 2012 | 22290644 |
| antimicrobial activity and chemical composition of essential oil from the seeds of artemisia aucheri boiss. | artemisia aerial parts are well known for antimicrobial activities including anti malaria. | 2012 | 24624145 |
| [analysis of international programs of the hygienic monitoring of foodborne pathogens]. | the article gives an overview of information international networks of monitoring the microbiological food safety in the world. the most famous are the european network of food safety (efsn), global network for disease surveillance, foodborne (gfn), international network for the determination of molecular subtypes of bacterial food patogens (pulsenet), international network of food safety (infosan). the network efsn is engaged development of information database and of warning systems to assess ... | 2012 | 23530431 |
| quantification of biofilm production on polystyrene by listeria, escherichia coli and staphylococcus aureus isolated from a poultry slaughterhouse. | this study assessed biofilm formation on polystyrene by staphylococcus aureus, listeria monocytogenes, l. welshimeri and escherichia coli, isolated from a slaughtering plant, grown on tryptic soy broth (tsb) using different glucose concentrations. the tested bacteria produced biofilm in at least one of the concentrations used, and some of them were strong biofilm producers. | 2010 | 24031590 |
| enteric microflora in ibd: pathogens or commensals? | : both pathogenic and normal enteric microflora can induce and perpetuate chronic intestinal inflammation with systemic manifestations in genetically susceptible hosts. at the present time, there is no convincing indication that the majority of cases of ulcerative colitis or crohn's disease is caused by persistent infection by mycobacterium paratuberculosis, measles, listeria monocytogenes, or helicobacter species, but this possibility remains a valid hypothesis. transient infection with any of ... | 1997 | 23282809 |
| in vivo assessment of natural killer cell responses during chronic feline immunodeficiency virus infection. | accumulating evidence suggests that natural killer (nk) cells may have an important role in hiv-1 disease pathogenesis; however, in vivo studies are lacking. feline immunodeficiency virus (fiv) infection of cats provides a valuable model to study nk cell function in vivo. the immune response against listeria monocytogenes (lm) is well characterized, allowing its use as an innate immune probe. we have previously shown that locally delivered il-15 can improve lm clearance in fiv-infected animals, ... | 2012 | 22701523 |
| a genetic screen reveals that synthesis of 1,4-dihydroxy-2-naphthoate (dhna), but not full-length menaquinone, is required for listeria monocytogenes cytosolic survival. | through unknown mechanisms, the host cytosol restricts bacterial colonization; therefore, only professional cytosolic pathogens are adapted to colonize this host environment. listeria monocytogenes is a gram-positive intracellular pathogen that is highly adapted to colonize the cytosol of both phagocytic and nonphagocytic cells. to identify l. monocytogenes determinants of cytosolic survival, we designed and executed a novel screen to isolate l. monocytogenes mutants with cytosolic survival defe ... | 2017 | 28325762 |
| manipulation of host membranes by the bacterial pathogens listeria, francisella, shigella and yersinia. | bacterial pathogens display an impressive arsenal of molecular mechanisms that allow survival in diverse host niches. subversion of plasma membrane and cytoskeletal functions are common themes associated to infection by both extracellular and intracellular pathogens. moreover, intracellular pathogens modify the structure/stability of their membrane-bound compartments and escape degradation from phagocytic or autophagic pathways. here, we review the manipulation of host membranes by listeria mono ... | 2016 | 27448494 |
| a stable live bacterial vaccine. | formulating vaccines into a dry form enhances its thermal stability. this is critical to prevent administering damaged and ineffective vaccines, and to reduce its final cost. a number of vaccines in the market as well as those being evaluated in the clinical setting are in a dry solid state; yet none of these vaccines have achieved long-term stability at high temperatures. we used spray-drying to formulate a recombinant live attenuated listeria monocytogenes (lm; expressing francisella tularensi ... | 2016 | 27020530 |
| listeria monocytogenes merr-like regulator nmlrlm: its transcriptome and role in stress response. | nmlr, a negative transcription regulator in the merr family, is involved in oxidative and nitrosative stress response in neisseria gonorrhoeae and haemophilus influenzae. in this study, the objective was to characterize the role and the regulon of nmlr in the foodborne listeria monocytogenes. an l. monocytogenes nmlr null mutant strain was constructed. transcriptomes of strain 10403s wild type (wt) and δnmlrlm strains grown to the stationary phase were determined by mrna sequencing. differential ... | 2016 | 27058117 |
| cerebrospinal fluid pcr analysis and biochemistry in bodies with severe decomposition. | the aim of this study was to assess whether neisseria meningitidis, listeria monocytogenes, streptococcus pneumoniae and haemophilus influenzae can be identified using the polymerase chain reaction technique in the cerebrospinal fluid of severely decomposed bodies with known, noninfectious causes of death or whether postmortem changes can lead to false positive results and thus erroneous diagnostic information. biochemical investigations, postmortem bacteriology and real-time polymerase chain re ... | 2015 | 25623190 |
| [rapid identification of meningitis due to bacterial pathogens]. | we constructed a new real-time pcr method to detect causative pathogens in cerebrospinal fluid (csf) from patient due to bacterial meningitis. the eight pathogens targeted in the pcr are streptococcus pneumoniae, haemophilus influenzae, streptococcus agalactiae, staphylococcus aurues, neisseria meningitides, listeria monocytogenes, esherichia coli, and mycoplasma pneumoniae. the total time from dna extraction from csf to pcr analysis was 1.5 hour. the pathogens were detected in 72% of the csf sa ... | 2013 | 24291923 |
| peptidoglycan maturation enzymes affect flagellar functionality in bacteria. | the flagellar machinery is a highly complex organelle composed of a free rotating flagellum and a fixed stator that converts energy into movement. the assembly of the flagella and the stator requires interactions with the peptidoglycan layer through which the organelle has to pass for externalization. lytic transglycosylases are peptidoglycan degrading enzymes that cleave the sugar backbone of peptidoglycan layer. we show that an endogenous lytic transglycosylase is required for full motility of ... | 2012 | 22994973 |
| differential modulation of intracellular survival of cytosolic and vacuolar pathogens by curcumin. | curcumin, a principal component of turmeric, acts as an immunomodulator regulating the host defenses in response to a diseased condition. the role of curcumin in controlling certain infectious diseases is highly controversial. it is known to alleviate symptoms of helicobacter pylori infection and exacerbate that of leishmania infection. we have evaluated the role of curcumin in modulating the fate of various intracellular bacterial pathogens. we show that pretreatment of macrophages with curcumi ... | 2012 | 22890770 |
| the mammalian microrna response to bacterial infections. | micrornas are small rnas that post-transcriptionally regulate eukaryotic gene expression. in addition to their involvement in a wide range of physiological and pathological processes, including viral infections, micrornas are increasingly implicated in the eukaryotic response to bacterial pathogens. recent studies have characterized changes in host microrna expression following infection with exclusively extracellular (helicobacter pylori) or intracellular (salmonella enterica) gram-negative bac ... | 2012 | 22664920 |
| helminth infections predispose mice to pneumococcal pneumonia but not to other pneumonic pathogens. | pneumonia is the leading killer of children worldwide. here, we report that helminth-infected mice develop fatal pneumonia when challenged with streptococcus pneumoniae. mice were chronically infected with either the flatworm taenia crassiceps or the roundworm heligmosomoides polygyrus. upon challenge with a pneumonic type 3 strain of s. pneumoniae (a66.1), the worm-infected mice developed pneumonia at a rate and to a degree higher than age-matched control mice as measured by bioluminescent imag ... | 2014 | 24952091 |
| nonviral oncogenic antigens and the inflammatory signals driving early cancer development as targets for cancer immunoprevention. | cancer immunoprevention is an emerging field that holds much promise. within the past 20 years, prophylactic vaccines have been implemented on the population level for the immunoprevention of carcinomas induced by viruses, specifically hepatitis b virus (hbv) and human papillomavirus (hpv) infection. armed with the success of prophylactic vaccines that prevent viral-induced tumors, the field must overcome its next hurdle: to develop robust prophylactic vaccines that prevent the remaining >80% of ... | 2015 | 25623216 |
| enhanced virus-specific cd8+ t cell responses by listeria monocytogenes-infected dendritic cells in the context of tim-3 blockade. | in this study, we engineered listeria monocytogens (lm) by deleting the lmδacta/δinlb virulence determinants and inserting hcv-ns5b consensus antigens to develop a therapeutic vaccine against hepatitis c virus (hcv) infection. we tested this recombinant lm-hcv vaccine in triggering of innate and adaptive immune responses in vitro using immune cells from hcv-infected and uninfected individuals. this live-attenuated lm-hcv vaccine could naturally infect human dendritic cells (dc), thereby driving ... | 2014 | 24498204 |
| occurrence of hepatitis a and e and norovirus gi and gii in ready-to-eat vegetables in italy. | fresh vegetables and their ready-to-eat (rte) salads have become increasingly recognized as potential vehicles for foodborne diseases. the eu reg. 1441/2007 establishes microbiological criteria for bacterial pathogens for products placed on the market during their shelf-life (i.e. salmonella spp., listeria monocytogenes) for pre-cut fruits and vegetables (rte) whilst it does not address the problem of contamination by enteric viruses. in this study we investigated the contamination by hepatitis ... | 2017 | 28319799 |
| impact of the prevalence of different pathogens on the performance of sampling plans in lettuce products. | prevalence and concentration of listeria monocytogenes, salmonella spp. and enteric pathogenic viruses (namely hepatitis a-hav, and noroviruses genogroup i-novgi and genogroup ii-novgii) were determined in raw and rte lettuce from a spanish processing premise. fifteen samplings were made from september 2010 to february 2012 (n=600 samples). sampling strategies for pathogen detection were suggested by the characterization of the uncertainty in prevalence associated with the performance of two-cla ... | 2014 | 24815427 |
| scoping the impact of changes in population age-structure on the future burden of foodborne disease in the netherlands, 2020-2060. | a demographic shift towards a larger proportion of elderly in the dutch population in the coming decades might change foodborne disease incidence and mortality. in the current study we focused on the age-specific changes in the occurrence of foodborne pathogens by combining age-specific demographic forecasts for 10-year periods between 2020 and 2060 with current age-specific infection probabilities for campylobacter spp., non-typhoidal salmonella, hepatitis a virus, acquired toxoplasma gondii an ... | 2013 | 23851976 |
| development of leprosy in a patient with rheumatoid arthritis during treatment with etanercept: a case report. | rheumatoid arthritis (ra) is a chronic, systemic inflammatory disorder. there is a clear association between some disease-modifying drugs used to treat ra and infection. the introduction of the anti-tumor necrosis factor (tnf) therapies has improved the outcome of severe ra. the tnf-antagonism may increase susceptibility to granulomatous pathogens such as mycobacterium tuberculosis, listeria monocytogenes, and histoplasma capsulatum. | 2012 | 22542278 |
| comprehensive proteome analysis of lysosomes reveals the diverse function of macrophages in immune responses. | phagocytosis and autophagy in macrophages have been shown to be essential to both innate and adaptive immunity. lysosomes are the main catabolic subcellular organelles responsible for degradation and recycling of both extracellular and intracellular material, which are the final steps in phagocytosis and autophagy. however, the molecular mechanisms underlying lysosomal functions after infection remain obscure. in this study, we conducted a quantitative proteomics analysis of the changes in const ... | 2017 | 28088779 |
| epidemiology of infectious encephalitis causes in 2016. | we performed a literature search in the medline database, using the pubmed website. the incidence of presumably infectious encephalitis is estimated at 1.5-7 cases/100,000 inhabitants/year, excluding epidemics. infectious encephalitis and immune-mediated encephalitis share similar clinical signs and symptoms. the latter accounts for a significant proportion of presumably infectious encephalitis cases without any established etiological diagnosis; as shown from a prospective cohort study where 21 ... | 2017 | 28341533 |
| management of adult infectious encephalitis in metropolitan france. | infectious encephalitis is a severe disease leading to a high mortality and morbidity. the most frequent causes include herpes simplex virus, varicella zoster virus, listeria monocytogenes, and mycobacterium tuberculosis. urgent treatment is required (anti-infective therapy and nonspecific supportive care). the aim of this study was to define treatment strategy, empirical and after microbiological documentation at 48hours, through a systematic literature review. | 2017 | 28336304 |
| serious infection during etanercept, infliximab and adalimumab therapy for rheumatoid arthritis: a literature review. | the purpose of this review is to establish whether there is a significantly increased incidence of serious infections during treatment for rheumatoid arthritis (ra) with etanercept, infliximab or adalimumab, to determine the background risk of serious infection in ra patients without treatment with any biological therapy and to ascertain which organisms are involved in serious infections in ra patients while being treated with etanercept, infliximab or adalimumab. randomised controlled trials (r ... | 2016 | 26200188 |
| attenuated listeria monocytogenes vectors overcome suppressive plasma factors during hiv infection to stimulate myeloid dendritic cells to promote adaptive immunity and reactivation of latent virus. | hiv-1 infection is characterized by myeloid dendritic cell (dc) dysfunction, which blunts the responsiveness to vaccine adjuvants. we previously showed that nonviral factors in hiv-seropositive plasma are partially responsible for mediating this immune suppression. in this study we investigated recombinant listeria monocytogenes (lm) vectors, which naturally infect and potently activate dcs from seronegative donors, as a means to overcome dc dysfunction associated with hiv infection. monocyte-de ... | 2015 | 25376024 |
| an attenuated listeria monocytogenes vector primes more potent simian immunodeficiency virus-specific mucosal immunity than dna vaccines in mice. | a human immunodeficiency virus type 1 (hiv-1) vaccine that induces potent immune responses in the gastrointestinal mucosa would be highly desirable. here we show that attenuated recombinant listeria monocytogenes, administered orally utilizing its natural route of infection, induces potent mucosal as well as systemic immune responses in mice. moreover, these responses can be boosted efficiently with replication-incompetent adenoviral vectors. l. monocytogenes elicited more potent simian immunode ... | 2013 | 23388715 |
| the extradomain a of fibronectin (eda) combined with poly(i:c) enhances the immune response to hiv-1 p24 protein and the protection against recombinant listeria monocytogenes-gag infection in the mouse model. | the development of effective vaccines against hiv-1 infection constitutes one of the major challenges in viral immunology. one of the protein candidates in vaccination against this virus is p24, since it is a conserved hiv antigen that has cytotoxic and helper t cell epitopes as well as b cell epitopes that may jointly confer enhanced protection against infection when used in immunization-challenge approaches. in this context, the adjuvant effect of eda (used as edap24 fusion protein) and poly(i ... | 2012 | 22326778 |
| adxs-hpv: a therapeutic listeria vaccination targeting cervical cancers expressing the hpv e7 antigen. | listeria monocytogenes is a bacterium that can be genetically modified to express fusion proteins with antigens specific to certain cancer models. this technology has been harnessed to develop adxs11-001, a vaccine that aims to elicit an immune response against human papillomavirus (hpv) oncoprotein e7. pre-clinical studies assessing the efficacy of recombinant listeria vaccination targeting this same oncoprotein have consistently demonstrated successful reduction of in vivo tumor burden among ... | 2014 | 25483687 |
| episomal expression of truncated listeriolysin o in lmdda-llo-e7 vaccine enhances antitumor efficacy by preferentially inducing expansions of cd4+foxp3- and cd8+ t cells. | studies have shown that listeria monocytogenes (lm)-based vaccine expressing a fusion protein comprising truncated listeriolysin o (llo) and human papilloma virus (hpv) e7 protein (lm-llo-e7) induces a decrease in regulatory t cells (treg) and complete regression of established, transplanted hpv-tc-1 tumors in mice. however, how the lm-based vaccine causes a decrease in tregs remains unclear. using a highly attenuated lm dal dat δacta strain (lmdda)-based vaccine, we report here that the vector ... | 2014 | 24872025 |
| interferon γ-induced intratumoral expression of cxcl9 alters the local distribution of t cells following immunotherapy with listeria monocytogenes. | the ability of listeria monocytogenes-based anticancer vaccines to induce tumor regression depends on the responsiveness of malignant cells to interferon γ (ifnγ). inhibition of ifnγ limits the recruitment of t cells to the tumors of vaccinated mice. we hypothesized that vaccination with immunotherapeutic l. monocytogenes induces the ifnγ-dependent production of chemokines that regulate the migration of tumor-infiltrating t cells. to gain further insights into this issue, we examined the chemoki ... | 2013 | 24083082 |
| listeria monocytogenes-derived listeriolysin o has pathogen-associated molecular pattern-like properties independent of its hemolytic ability. | there is a constant need for improved adjuvants to augment the induction of immune responses against tumor-associated antigens (taa) during immunotherapy. previous studies have established that listeriolysin o (llo), a cholesterol-dependent cytolysin derived from listeria monocytogenes, exhibits multifaceted effects to boost the stimulation of immune responses to a variety of antigens. however, the direct ability of llo as an adjuvant and whether it acts as a pathogen-associated molecular patter ... | 2013 | 23136118 |
| prophylactic and therapeutic efficacy of an attenuated listeria monocytogenes-based vaccine delivering hpv16 e7 in a mouse model. | listeria monocytogenes (l. monocytogenes) has been developed as a cancer vaccine vector due to its ability to elicit strong innate and adaptive immune responses. for clinical application, it is necessary to exploit a listeria platform strain that is safe and that also retains its immunogenicity to develop vaccine candidates against cancer. in this study, a highly attenuated strain with a deletion of acta/plcb was employed as a vector to deliver the human papillomavirus type 16 (hpv16) e7 antigen ... | 2012 | 23027427 |
| adap plays a pivotal role in cd4+ t cell activation but is only marginally involved in cd8+ t cell activation, differentiation, and immunity to pathogens. | the adhesion and degranulation promoting adaptor protein (adap) is a multifunctional scaffold involved in many different signaling pathways that are important for the function of t cells, including the inside-out and outside-in signaling of integrins, the activation of nf-κb, and the subsequent production of proinflammatory cytokines (e.g., ifn-γ and il-2). strikingly, despite its well-established role in t cells, previous studies did not distinguish between cd4(+) and cd8(+) t cells, and thus, ... | 2017 | 27605210 |
| comparison of microbiological and probiotic characteristics of lactobacilli isolates from dairy food products and animal rumen contents. | lactobacilli are employed in probiotic food preparations and as feed additives in poultry and livestock, due to health benefits associated with their consumption. the objective of this study was to evaluate and compare the probiotic potential of ten lactobacilli strains isolated from commercial dairy food products and animal rumen contents in new zealand. genetic identification of the isolates revealed that all belonged to the genus lactobacillus, specifically the species l. reuteri, l. rhamnosu ... | 2015 | 27682086 |
| antimicrobial hyaluronic acid-cefoxitin sodium thin films produced by electrospraying. | the healing properties of hyaluronic acid (ha) in the recovery of wounds are well known. cefoxitin (cef), a cephalosporin antibiotic, is generally used to prevent and treat postoperative infections. in this study, we describe the incorporation of cef in ha thin films (cef-haf) by using electrospraying. scanning electron microscopy images showed that ha-containing thin films (haf) were composed of numerous nanoparticles (255 ± 177 nm in diameter) with irregular surfaces, connected to each other w ... | 2016 | 27146506 |
| chemical composition and antibacterial activity of some medicinal plants from lamiaceae family. | chemical composition and antibacterial activity of aqueous (ethanolic and methanolic) extracts from herbs often used in polish cuisine and traditional herbal medicine including thyme (thymus vulgaris l.), rosemary (rosmarinus officinalis l.), oregano (origanum vulgare l.), peppermint (mentha piperita l.) and sage (salvia officinalis l.) were compared. the aqueous ethanolic extracts contained slightly higher levels of phenolics compared to the aqueous methanolic extracts. in turn, gc-ms analysis ... | 2015 | 26647633 |
| listeria monocytogenes la111 and klebsiella pneumoniae kctc 2242: shine-dalgarno sequences. | listeria monocytogenes can cause serious infection and recently, relapse of listeriosis has been reported in leukemia and colorectal cancer, and the patients with klebsiella pneumoniae are at increased risk of colorectal cancer. translation initiation codon recognition is basically mediated by shine-dalgarno (sd) and the anti-sd sequences at the small ribosomal rna (ssu rrna). in this research, shine-dalgarno sequences prediction in listeria monocytogenes la111 and klebsiella pneumoniae kctc 224 ... | 2014 | 24551820 |
| antilisterial bacteriocin from lactobacillus rhamnosus cjnu 0519 presenting a narrow antimicrobial spectrum. | a lactic acid bacterium presenting antimicrobial activity against a lactobacillus acidophilus strain used for eradication of acid inhibition was isolated from a natural cheese. the 16s rrna gene sequence of the isolate best matched with a strain of l. rhamnosus and was designated l. rhamnosus cjnu 0519. the antimicrobial activity of the partially purified bacteriocin of cjnu 0519 was abolished when treated with a protease, indicating the protein nature of the bacteriocin. the partially purified ... | 2015 | 26761811 |
| probiotic-mediated competition, exclusion and displacement in biofilm formation by food-borne pathogens. | the objective of this study was to examine the inhibitory effect of probiotic strains on pathogenic biofilm formation in terms of competition, exclusion and displacement. probiotic strains (lactobacillus acidophilus kacc 12419, lact. casei kacc 12413, lact. paracasei kacc 12427 and lact. rhamnosus kacc 11953) and pathogens (salmonella typhimurium kccm 40253 and listeria monocytogenes kacc 12671) were used to evaluate the auto-aggregation, hydrophobicity and biofilm formation inhibition. the high ... | 2013 | 23362863 |
| physicochemical and hygienic effects of lactobacillus acidophilus in iranian white cheese. | increasing incidence of food-borne disease along with its social and economic consequences have led to conducting extensive research in order to produce safer food and develop new antimicrobial agents; among them, extensive use of probiotics and bacteriocins as biological additives is of significant importance. the aim of the present study was to evaluate the interactions (growth behavior and survival) of listeria monocytogenes and lactobacillus acidophilus in various stages of production, ripen ... | 2012 | 25610568 |
| role of lactic acid bacteria as a biosanitizer to prevent attachment of listeria monocytogenes f6900 on deli slicer contact surfaces. | the study was conducted to evaluate the attachment of three lactic acid bacteria (lab) strains and their combination in a cocktail, to stainless steel coupons from a deli slicer, and their ability to inhibit the attachment of listeria monocytogenes. in a previous study, three lab strains, pediococcus acidilactici, lactobacillus amylovorus, and lactobacillus animalis, were isolated from ready-to-eat meat and exhibited antilisterial effect. in the study reported here, hydrophobicity tests were det ... | 2012 | 22856566 |
| use of potential probiotic lactic acid bacteria (lab) biofilms for the control of listeria monocytogenes, salmonella typhimurium, and escherichia coli o157:h7 biofilms formation. | use of probiotic biofilms can be an alternative approach for reducing the formation of pathogenic biofilms in food industries. the aims of this study were (i) to evaluate the probiotic properties of bacteriocinogenic (lactococcus lactis vb69, l. lactis vb94, lactobacillus sakei mbsa1, and lactobacillus curvatus mbsa3) and non-bacteriocinogenic (l. lactis 368, lactobacillus helveticus 354, lactobacillus casei 40, and weissela viridescens 113) lactic acid bacteria (lab) isolated from brazilian's f ... | 2016 | 27375584 |
| functional properties of peanut fractions on the growth of probiotics and foodborne bacterial pathogens. | various compounds found in peanut (arachis hypogaea) have been shown to provide multiple benefits to human health and may influence the growth of a broad range of gut bacteria. in this study, we investigated the effects of peanut white kernel and peanut skin on 3 strains of lactobacillus and 3 major foodborne enteric bacterial pathogens. significant (p < 0.05) growth stimulation of lactobacillus casei and lactobacillus rhamnosus was observed in the presence of 0.5% peanut flour (pf) made from pe ... | 2015 | 25627431 |
| lack of correlation between in vitro antibiosis and in vivo protection against enteropathogenic bacteria by probiotic lactobacilli. | increased resistance to infection is one of the beneficial effects attributed to probiotic microorganisms. this effect may be due to several mechanisms: production of inhibitory substances, blocking of adhesion sites on the intestinal surface, competition for nutrients and stimulation of mucosal and systemic immunity. the present study aimed to investigate the correlation between in vitro and in vivo antimicrobial activity of probiotic lactobacilli. the agar spot test was used to show that twent ... | 2014 | 24140789 |
| increase of the adhesion ability and display of a rumen fungal xylanase on the cell surface of lactobacillus casei by using a listerial cell-wall-anchoring protein. | lactobacillus, which has great adhesion ability to intestinal mucosa and is able to hydrolyse plant cell walls, can be used more efficiently as a feed additive. to increase the adhesion ability and display a fungal xylanase on the cell surface of lactobacillus casei, the listeria monocytogenes cell-wall-anchoring protein gene, mub, was introduced into l. casei atcc 393 cells and used as a fusion partner to display the rumen fungal xylanase xyncdbfv on the cell surface of the transformed strains. | 2014 | 23824609 |
| impact of lactobacilli on orally acquired listeriosis. | listeria monocytogenes is a foodborne pathogen that crosses the intestinal barrier and disseminates within the host. here, we report a unique comprehensive analysis of the impact of two lactobacillus species, lactobacillus paracasei cncm i-3689 and lactobacillus casei bl23, on l. monocytogenes and orally acquired listeriosis in a gnotobiotic humanized mouse model. we first assessed the effect of treatment with each lactobacillus on l. monocytogenes counts in host tissues and showed that each dec ... | 2012 | 23012479 |