| assessment of the microbiological safety of precut fruit from retail and catering premises in the united kingdom. | fresh fruit has been associated with a number of foodborne outbreaks in recent years. in particular, a large outbreak of listeriosis in the united states in 2011 was associated with consumption of cantaloupe melon, and an outbreak of salmonella newport in the united kingdom and europe (also in 2011) was linked to watermelon consumption. a study of precut fruit products from catering and retail premises in the united kingdom was, therefore, carried out to assess their microbiological safety. betw ... | 2016 | 27052864 |
| effect of iranian ziziphus honey on growth of some foodborne pathogens. | honey has previously been shown to have wound healing and antimicrobial properties, but this is dependent on the type of honey, geographical location, and flower from which the final product is derived. we tested the antimicrobial activity of a natural honey originating from the ziziphus spina-christi tree, against selected strains of bacteria. ziziphus honey among more than a 100 verities of honey is known to have the greatest value of energy and minerals in it. | 2017 | 27003970 |
| strategies to block bacterial pathogenesis by interference with motility and chemotaxis. | infections by motile, pathogenic bacteria, such as campylobacter species, clostridium species, escherichia coli, helicobacter pylori, listeria monocytogenes, neisseria gonorrhoeae, pseudomonas aeruginosa, salmonella species, vibrio cholerae, and yersinia species, represent a severe economic and health problem worldwide. of special importance in this context is the increasing emergence and spread of multidrug-resistant bacteria. due to the shortage of effective antibiotics for the treatment of in ... | 2016 | 27000091 |
| integration of antimicrobial pectin-based edible coating and active modified atmosphere packaging to preserve the quality and microbial safety of fresh-cut persimmon (diospyros kaki thunb. cv. rojo brillante). | the greatest hurdle to the commercial marketing of fresh-cut fruits is related to their higher susceptibility to enzymatic browning, tissue softening, and microbial growth. the aim of this study was to test the efficacy of a pectin-based edible coating and low oxygen modified atmosphere packaging (map) to control enzymatic browning and reduce microbial growth of fresh-cut 'rojo brillante' persimmon. the survival of escherichia coli, salmonella enteritidis and listeria monocytogenes artificially ... | 2016 | 26997097 |
| an economic evaluation of pulsenet: a network for foodborne disease surveillance. | the pulsenet surveillance system is a molecular subtyping network of public health and food regulatory agency laboratories designed to identify and facilitate investigation of foodborne illness outbreaks. this study estimates health and economic impacts associated with pulsenet. the staggered adoption of pulsenet across the states offers a natural experiment to evaluate its effectiveness, which is measured as reduction of reported illnesses due to improved information, enhanced industry accounta ... | 2016 | 26993535 |
| atf3 provides protection from staphylococcus aureus and listeria monocytogenes infections. | activating transcription factor 3 (atf3) is a stress-induced transcriptional regulator in eukaryote. the role of atf3 in cancer has been well defined, but how atf3 functions in bacterial infection is not well understood. pneumococcal infection has been shown to induce atf3 expression, which subsequently enhances cytokine production and provides protection from lethal streptococcus pneumoniae infection, but the role of atf3 in other gram-positive (g(+)) infections remains unclear. here, we report ... | 2016 | 26989156 |
| effect of high intensity ultrasound on the fermentation profile of lactobacillus sakei in a meat model system. | the objective of this study was to investigate the efficacy of high intensity ultrasound on the fermentation profile of lactobacillus sakei in a meat model system. ultrasound power level (0-68.5 w) and sonication time (0-9 min) at 20 °c were assessed against the growth of l. sakei using a microplate reader over a period of 24h. the l. sakei growth data showed a good fit with the gompertz model (r(2)>0.90; se<0.042). second order polynomial models demonstrated the effect of ultrasonic power and s ... | 2016 | 26964981 |
| foodborne outbreaks reported to the u.s. food safety and inspection service, fiscal years 2007 through 2012. | the u.s. department of agriculture's food safety and inspection service (fsis) works closely with federal, state, and local public health partners to investigate foodborne illness outbreaks associated with its regulated products. to provide insight into outbreaks associated with meat and poultry, outbreaks reported to fsis during fiscal years 2007 through 2012 were evaluated. outbreaks were classified according to the strength of evidence linking them to an fsis-regulated product and by their ep ... | 2016 | 26939654 |
| assessing the impacts of temperature and storage on escherichia coli, salmonella, and l. monocytogenes decay in dairy manure. | elevated levels of animal waste-borne pathogen in ambient water is a serious human health issue. mitigating influx of pathogens from animal waste such as dairy manure to soil and water requires improving our existing knowledge of pathogen reductions in dairy manure treatment methods. this study was conducted to enhance the understanding of human pathogen decay in liquid dairy manure in anaerobic (an) and limited aerobic (la) storage conditions. the decay of three pathogens (escherichia coli, sa ... | 2016 | 26922419 |
| microbiological quality of fresh produce obtained from retail stores on the eastern shore of maryland, united states of america. | the aim of this study was to investigate the microbiological quality of six types of fresh produce obtained from three retail stores located on the eastern shore of maryland, usa. a total of 414 samples representing basil, cilantro, lettuce, scallion, spinach, and parsley were analyzed for total aerobic bacteria (apc), total coliforms, escherichia coli, and three pathogenic bacteria (e. coli o157:h7, listeria monocytogenes, and salmonella), using standard methods. presumptive pathogenic isolates ... | 2016 | 26919815 |
| systemic analysis of foodborne disease outbreak in korea. | this study systemically analyzed data on the prevalence of foodborne pathogens and foodborne disease outbreaks to identify the priorities of foodborne infection risk management in korea. multiple correspondence analysis was applied to three variables: origin of food source, phase of food supply chain, and 12 pathogens using 358 cases from 76 original papers and official reports published in 1998-2012. in addition, correspondence analysis of two variables--place and pathogen--was conducted based ... | 2016 | 26863429 |
| establishment and application of a visual dna microarray for the detection of food-borne pathogens. | the accurate detection and identification of food-borne pathogenic microorganisms is critical for food safety nowadays. in the present work, a visual dna microarray was established and applied to detect pathogens commonly found in food, including salmonella enterica, shigella flexneri, e. coli o157:h7 and listeria monocytogenes in food samples. multiplex pcr (mpcr) was employed to simultaneously amplify specific gene fragments, fimy for salmonella, ipah for shigella, iap for l. monocytogenes and ... | 2016 | 26860568 |
| identification of the risk factors associated with cheese production to implement the hazard analysis and critical control points (haccp) system on cheese farms. | the purpose of this paper was to evaluate, by statistical analyses, risk factors on cheese farms that can influence the microbial contamination of their products. various assessment tools, such as cheese production questionnaires, food handlers' knowledge testing, and hygiene assessment system surveys, were used on 39 cheese farms on the island of gran canaria, spain. the microbiological status of 773 raw milk and cheese samples from the cheese farms was assessed by enumerating total viable coun ... | 2016 | 26851842 |
| inactivation of escherichia coli, listeria monocytogenes, and salmonella enteritidis by cymbopogon citratus d.c. stapf. essential oil in pineapple juice. | in the present study, the efficacy of cymbopogon citratus d.c. stapf. essential oil (cceo) to provoke a 5-log cfu/ml (5-log) inactivation in a mixed composite of escherichia coli, listeria monocytogenes, and salmonella enterica serovar enteritidis in pineapple (ananas comosus (l.) merril) juice (4°c) was assessed. moreover, the effects of cceo on the physicochemical and sensory quality parameters of pineapple juice were evaluated. the mic of cceo was 5 μl/ml against the composite mix examined. f ... | 2016 | 26818981 |
| the response of foodborne pathogens to osmotic and desiccation stresses in the food chain. | in combination with other strategies, hyperosmolarity and desiccation are frequently used by the food processing industry as a means to prevent bacterial proliferation, and particularly that of foodborne pathogens, in food products. however, it is increasingly observed that bacteria, including human pathogens, encode mechanisms to survive and withstand these stresses. this review provides an overview of the mechanisms employed by salmonella spp., shiga toxin producing e. coli, cronobacter spp., ... | 2016 | 26803272 |
| edible antimicrobial films based on microencapsulated lemongrass oil. | edible films and coatings have been proposed as viable alternatives for the preservation of fresh food such as fruit, meat, fish and cheese. they can be designed to contain natural antioxidants, vitamins and antimicrobials in order to extend shelf life of the product keeping the natural sensorial properties. essential oils have been targeted as potential active principles for edible films and coatings given their well-recognized antioxidant, antimicrobial and sensory properties. in the present w ... | 2016 | 26788005 |
| combined effect of irradiation and ageing condition on physicochemical and microbial quality of hanwoo eye of round. | the combined effects of electron-beam irradiation and ageing of beef were examined. the irradiated samples at dose of 0 or 2 kgy were kept and analyzed for the microbial growth, shear values, meat color, and nucleotide-related flavor compounds at different ageing temperatures (2, 10, or 25℃) for 8 d. the irradiation effect on inactivation of foodborne pathogens was also investigated. the population of listeria monocytogenes and e. coli o157:h7 inoculated in beef samples decreased in proportion t ... | 2015 | 26761855 |
| electrochemical genosensing of salmonella, listeria and escherichia coli on silica magnetic particles. | a magneto-genosensing approach for the detection of the three most common pathogenic bacteria in food safety, such as salmonella, listeria and escherichia coli is presented. the methodology is based on the detection of the tagged amplified dna obtained by single-tagging pcr with a set of specific primers for each pathogen, followed by electrochemical magneto-genosensing on silica magnetic particles. a set of primers were selected for the amplification of the inva (278 bp), prfa (217 bp) and eaea ... | 2016 | 26724759 |
| isotopologue profiling of the listerial n-metabolism. | the nitrogen (n-) sources and the relative contribution of a nitrogenous nutrient to the n-pool of the gram-positive pathogen listeria monocytogenes are largely unknown. therefore, (15) n-isotopologue profiling was established to study the n-metabolism of l. monocytogenes. the pathogen was grown in a defined minimal medium supplemented with potential (15) n-labeled nutrients. the bacteria were harvested and hydrolysed under acidic conditions, and the resulting amino acids were analysed by gc-ms, ... | 2016 | 26699934 |
| impact of t300a variant of atg16l1 on antibacterial response, risk of culture positive infections, and clinical course of crohn's disease. | autophagy-related 16-like 1 (atg16l1) deficiency leads to impaired cellular autophagy and bacterial degradation as well as an altered cytokine production. the single-nucleotide polymorphism rs2241880 (t300a) is associated with an increased risk for crohn's disease (cd). atg16l1 polymorphisms could therefore have an impact on the risk of infectious complications and disease course in cd. we examined the impact of the t300a genotype on the antibacterial response toward a panel of pathogenic bacter ... | 2015 | 26673830 |
| real-time investigation of antibiotics-induced oxidative stress and superoxide release in bacteria using an electrochemical biosensor. | the involvement of oxidative stress in the mechanism of antibiotics-meditated cell death is unclear and subject to debate. the kinetic profile and a quantitative relationship between the release of reactive oxygen species (ros), bacteria and antibiotic type remain elusive. here we report direct measurements and analytical quantification of the release of superoxide radicals (o2(·-)), a major contributor to ros, in antibiotics-treated bacterial cultures using a cytochrome c electrochemical biosen ... | 2016 | 26655038 |
| biofabrication of zinc oxide nanoparticles using fruit extract of rosa canina and their toxic potential against bacteria: a mechanistic approach. | the use of plant extract in the biosynthesis of nanoparticles (nps) can be an eco-friendly approach and have been suggested as a possible alternative to classic methods namely physical and chemical procedures. in this study, the biosynthesis of zinc oxide (zno) nps by both "conventional heating" (ch) and "microwave irradiation" (mi) methods has been reported. stable and spherical znonps were produced using zinc nitrate and flesh extract of rosa canina fruit (rosehip) which was used as a precurso ... | 2016 | 26652376 |
| bactericidal action mechanism of negatively charged food grade clove oil nanoemulsions. | clove oil (co) anionic nanoemulsions were prepared with varying ratios of co to canola oil (ca), emulsified and stabilized with purity gum ultra (pgu), a newly developed succinylated waxy maize starch. interfacial tension measurements showed that co acted as a co-surfactant and there was a gradual decrease in interfacial tension which favored the formation of small droplet sizes on homogenization until a critical limit (5:5% v/v co:ca) was reached. antimicrobial activity of the negatively charge ... | 2016 | 26616926 |
| the effect of nacl reduction in the microbiological quality of cracked green table olives of the maçanilha algarvia cultivar. | the present work aimed at studying the effect of the partial replacement of nacl with kcl and cacl2 of the fermenting brines on the microbiological quality of natural cracked green maçanilha algarvia table olives. olives were fermented in different salt combinations (brine 1-8% nacl, brine 2-4% nacl 4% kcl, brine 3-4% nacl 4% cacl2, brine 4-4% kcl 4% cacl2, and brine 5-2.7% nacl 2.7% kcl 2.7% cacl2) and the abundance of yeasts and enterobacteria was determined. at the end of fermentation, the ma ... | 2016 | 26613162 |
| technological properties and probiotic potential of lactobacillus fermentum strains isolated from west african fermented millet dough. | throughout africa, food fermentations are still driven by indigenous microorganisms which influence the nutritional, organoleptic and safety of the final products. however, for improved safety, consistent quality and beneficial health effects, a trend has emerged which involves the isolation of indigenous strains from traditional fermented products to be used as functional starter cultures. these functional starter cultures possess inherent functional characteristics and can contribute to food q ... | 2015 | 26560346 |
| vital signs: multistate foodborne outbreaks - united states, 2010-2014. | millions of u.s. residents become ill from foodborne pathogens each year. most foodborne outbreaks occur among small groups of persons in a localized area. however, because many foods are distributed widely and rapidly, and because detection methods have improved, outbreaks that occur in multiple states and that even span the entire country are being recognized with increasing frequency. | 2015 | 26540483 |
| uv-heat treatments for the control of foodborne microbial pathogens in chicken broth. | this investigation established the process criteria for using uv-c light and mild heat (uv-h treatment) to inactivate 5-log10 cycles (performance criterion) of common foodborne pathogen populations, escherichia coli, salmonella typhimurium, listeria monocytogenes, and staphylococcus aureus, when inoculated in chicken broth. to define the target microorganism and the proper uv-h treatment conditions (including uv dose, treatment time, and temperature) that would achieve the stated performance cri ... | 2015 | 26539493 |
| characterization of a transferable bcrabc and cadac genes-harboring plasmid in listeria monocytogenes strain isolated from food products of animal origin. | in this study, we characterized a bcrabc cassette and its genetic environment harbored by a plasmid in listeria monocytogenes (l. monocytogenes) 11gzl18, a strain isolated from raw meat in 2011. the bcrabc cassette and its genetic environment were characterized, with a total of 33,727 nt nucleotide sequence obtained. the nucleotide sequences of the bcrabc cassette in strain 11gzl18 exhibited 100% identity to that on plasmid plm80, which is harbored by l. monocytogenes strain h7550 and h7858, and ... | 2016 | 26513251 |
| lactobacillus plantarum lb95 impairs the virulence potential of gram-positive and gram-negative food-borne pathogens in ht-29 and vero cell cultures. | listeria monocytogenes, salmonella enterica and verocytotoxigenic escherichia coli (vtec) are amongst the most important agents responsible for food outbreaks occurring worldwide. in this work, two lactobacillus spp. strains (labs), lactobacillus plantarum (lb95) and lactobacillus paraplantarum (lb13), previously isolated from spontaneously fermenting olive brines, and two reference probiotic strains, lactobacillus casei shirota and lactobacillus rhamnosus gg, were investigated for their ability ... | 2016 | 26506821 |
| development of prototypes of bioactive packaging materials based on immobilized bacteriophages for control of growth of bacterial pathogens in foods. | due to lack of adequate control methods to prevent contamination in fresh produce and growing consumer demand for natural products, the use of bacteriophages has emerged as a promising approach to enhance safety of these foods. this study sought to control listeria monocytogenes in cantaloupes and rte meat and escherichia coli o104:h4 in alfalfa seeds and sprouts under different storage conditions by using specific lytic bacteriophage cocktails applied either free or immobilized. bacteriophage c ... | 2016 | 26490649 |
| pathogen-nematode interaction: nitrogen supply of listeria monocytogenes during growth in caenorhabditis elegans. | listeria monocytogenes is a gram-positive facultatively intracellular human pathogen. due to its saprophytic lifestyle, l. monocytogenes is assumed to infect and proliferate within soil organisms such as caenorhabditis elegans. however, little is known about the nutrient usages and metabolite fluxes in this bacterium-nematode interaction. here, we established a nematode colonization model for l. monocytogenes and a method for the efficient separation of the pathogen from the nematodal gut. follo ... | 2016 | 26478569 |
| development of bioactive fish gelatin/chitosan nanoparticles composite films with antimicrobial properties. | the objective of this work was to develop active bio-based nanocomposite films from fish gelatin (fg) and chitosan nanoparticles (csnps) incorporated with origanum vulgare l. essential oil (oeo). csnps were obtained by ionic gelation of chitosan with sodium tripolyphosphate, which presented a spherical morphology with size range of 40-80nm. remarkable differences in the surface morphology were observed between the control and bioactive nanocomposite films as revealed by sem and afm images. ftir ... | 2016 | 26471681 |
| a small-scale experiment of using phage-based probiotic dietary supplement for prevention of e. coli traveler's diarrhea. | traveler's diarrhea (td) is caused by escherichia coli in 30% of cases. we have developed a phage cocktail for prophylaxis of td caused by e.coli, shigella flexneri, shigella sonnei, salmonella enterica, listeria monocytogenes or staphylococcus aureus, and investigated its effectiveness against infection caused by the non-pathogenic lac (-) strain of e.coli k12 c600 in animal and human trials. on the 6th day of both animal and human trials e. coli k12 c600 strain was detected in titer of 10(4) c ... | 2017 | 26458758 |
| in vitro generation of rabbit anti-listeria monocytogenes monoclonal antibody using single cell based rt-pcr linked cell-free expression systems. | rabbit monoclonal antibodies (mabs) have advantages over mouse antibodies in diagnostics and biotechnological applications owing to higher affinity and specificity.we developed a platform to generate rabbit mabs by a novel monoclonal antibody generation method named “single-cell reverse transcription-pcr linked in vitro-expression (sicrex)” system. in this method,we use single-cell based rt-pcr followed by sequential pcr steps of mab genes and subsequent cell-free protein synthesis (cfps) by usi ... | 2015 | 26454028 |
| survival and high-hydrostatic pressure inactivation of foodborne pathogens in salmorejo, a traditional ready-to-eat food. | salmorejo is a traditional tomato-based creamy product. because salmorejo is not heat-processed, there is a risk of contamination with foodborne pathogens from raw materials. even though bacterial growth in salmorejo is strongly inhibited because of its acidic ph (close to 3.9), the growth and survival of 3 foodborne pathogens in this food has not been studied before. in this study, 3 cocktails consisting of escherichia coli o157, salmonella enterica serovar enteritidis, and listeria monocytogen ... | 2015 | 26448479 |
| astaxanthin from psychrotrophic sphingomonas faeni exhibits antagonism against food-spoilage bacteria at low temperatures. | food production and processing industry holds a perpetual relationship with microorganisms and their by-products. in the present study, we aimed to identify beneficial cold-adapted bacteria devoid of any food spoilage properties and study their antagonism against common food-borne pathogens at low temperature conditions. ten isolates were obtained on selective isolation at 5 °c, which were spread across genera pseudomonas, sphingomonas, psychrobacter, leuconostoc, rhodococcus, and arthrobacter. ... | 2015 | 26411893 |
| persistence of indicator and pathogenic microorganisms in broccoli following manure spreading and irrigation with fecally contaminated water: field experiment. | in 2011 and 2012, trials consisting of experimental plots were carried out to evaluate the presence of pathogenic (listeria monocytogenes, salmonella) and prevalence of indicator (escherichia coli) microorganisms in broccoli fertilized with liquid hog manure or mineral fertilizers and irrigated zero, one, or two times with e. coli-contaminated water. in 2011, results showed that e. coli contamination in broccoli heads was affected by the interval between irrigation and sampling (p = 0.0236), wit ... | 2015 | 26408125 |
| the noa gene is functionally linked to the activation of the toll/imd signaling pathways in bactrocera dorsalis (hendel). | the noa gene is an essential gene encoding a very long chain fatty acid elongase. in this study, we cloned the noa gene of bactrocera dorsalis, which encodes a protein sharing 84.50% identity to the noa in drosophila melanogaster. the expression profiles indicated that the transcriptional level of noa was high at the egg stage and in the testis tissue. the results showed that noa expression was up-regulated after listeria monocytogenes, staphylococcus aureus and escherichia coli infection. silen ... | 2016 | 26404497 |
| improvement of antimicrobial activity of pediocin pa-1 by site-directed mutagenesis in c-terminal domain. | pediocin pa-1 is a well-known class iia bacteriocin which shows strong inhibitory effect against listeria monocytogenes. in this work, in order to improve the antimicrobial activity, eight single- site mutants and six combination mutants on nine interesting sites in the c-terminal region of pediocin pa-1 were constructed and expressed in escherichia coli heterologously. ten mutants demonstrated enhancement activity when performed the agar diffusion test to the indicator strain l. monocytogenes. ... | 2015 | 26299998 |
| control of escherichia coli and listeria monocytogenes in suckling-lamb meat evaluated using microbial challenge tests. | escherichia coli and listeria monocytogenes microbial challenge tests were performed on fresh suckling-lamb meat. hind leg slices were chilly stored under two modified atmosphere packaging (map) environments (a: 15%o2/60%co2/25%n2, b: 15%o2/30%co2/55%n2) and vacuum packaging (v). only e. coli was reduced between 0.72-1.25 log cfu/g from day 1 to day 4 by the combined use of map/v, chilling storage and the growth of native lactic acid bacteria. however, l. monocytogenes was not inhibited by the a ... | 2015 | 26298670 |
| streptococcus gallolyticus (bovis): a rare presentation of meningitis in the ed. | bacterial meningitis is a fairly common and often deadly manifestation of altered mental status in the elderly, carrying a mortality rate of greater than 20% despite antibiotic therapy. most commonly caused by streptococcus pneumoniae, listeria monocytogenes, escherichia coli, and klebsiella pneumoniae. we present a case of meningitis caused by streptococcus gallolyticus in an elderly, otherwise healthy woman. there have been no reports in the emergency medicine literature and only a few reports ... | 2016 | 26259921 |
| ixodes ricinus defensins attack distantly-related pathogens. | antimicrobial peptides are ubiquitous components of eukaryotic innate immunity. defensins are a well-known family of antimicrobial peptides, widely distributed in ticks, insects, plants and mammals, showing activity against bacteria, viruses, fungi, yeast and protozoan parasites. ixodes ricinus is the most common tick species in europe and is a vector of pathogens affecting human and animal health. recently, six defensins (including two isoforms) were identified in i. ricinus. we investigated th ... | 2015 | 26255244 |
| a microfluidic droplet digital pcr for simultaneous detection of pathogenic escherichia coli o157 and listeria monocytogenes. | sensitive and rapid identification of pathogenic bacterial is extremely important due to the serious threat of pathogens to human health. in this study, we demonstrate the simultaneous and sensitive detection of pathogenic escherichia coli o157 and listeria monocytogenes using a novel duplex droplet digital pcr (ddpcr) platform. the ddpcr platform, which uses a mineral oil-saturated polydimethylsiloxane (osp) chip to overcome the problem of droplet evaporation, integrates the functions of drople ... | 2015 | 26226346 |
| determining the minimum ripening time of artisanal minas cheese, a traditional brazilian cheese. | physical, physicochemical, and microbiological changes were monitored in 256 samples of artisanal minas cheese from eight producers from serro region (minas gerais, brazil) for 64 days of ripening to determine the minimum ripening time for the cheese to reach the safe microbiological limits established by brazilian legislation. the cheeses were produced between dry season (april-september) and rainy season (october-march); 128 cheeses were ripened at room temperature (25 ± 4 °c), and 128 were ri ... | 2015 | 26221111 |
| simultaneous electrochemical magneto genosensing of foodborne bacteria based on triple-tagging multiplex amplification. | simultaneous detection of salmonella enterica, listeria monocytogenes and escherichia coli based on triple-tagging multiplex pcr and electrochemical magneto genosensing on silica magnetic particles is reported. a set of tagging primers were selected for the specific amplification of yfir (375 bp), hlya (234 bp) and eaea (151bp), being one of the primers for each set labelled with fluorescein, biotin and digoxigenin coding for s. enterica, l. monocytogenes and e. coli, respectively. afterwards, e ... | 2015 | 26201982 |
| microbiological safety and food handling practices of seed sprout products in the australian state of victoria. | seed sprouts have been implicated as vehicles for numerous foodborne outbreaks worldwide. seed sprouts pose a unique food safety concern because of the ease of microbiological seed contamination, the inherent ability of the sprouting process to support microbial growth, and their consumption either raw or lightly cooked. to examine seed sprout safety in the australian state of victoria, a survey was conducted to detect specific microbes in seed sprout samples and to investigate food handling pra ... | 2015 | 26197293 |
| molecular insights into a molluscan transferrin homolog identified from disk abalone (haliotis discus discus) evidencing its detectable role in host antibacterial defense. | the basic function of transferrin is to bind iron (iii) ions in the medium and to deliver them to the locations where they are required for metabolic processes. it also takes part in the host immune defense mainly via its ability to bind to iron (iii) ions. hence, transferrin is also identified as an important acute-phase protein in host immunity. abalones are major shellfish aquaculture crops that are susceptible to a range of marine microbial infections. since transferrin is known to be a majo ... | 2015 | 26191782 |
| preparation and application of agar/alginate/collagen ternary blend functional food packaging films. | ternary blend agar/alginate/collagen (a/a/c) hydrogel films with silver nanoparticles (agnps) and grapefruit seed extract (gse) were prepared. their performance properties, transparency, tensile strength (ts), water vapor permeability (wvp), water contact angle (ca), water swelling ratio (sr), water solubility (ws), and antimicrobial activity were determined. the a/a/c film was highly transparent, and both agnps and gse incorporated blend films (a/a/c(agnps) and a/a/c(gse)) exhibited uv-screenin ... | 2015 | 26187189 |
| laminarin from irish brown seaweeds ascophyllum nodosum and laminaria hyperborea: ultrasound assisted extraction, characterization and bioactivity. | ultrasound assisted extraction (uae), purification, characterization and antioxidant activity of laminarin from irish brown seaweeds ascophyllum nodosum and laminarina hyperborea were investigated. uae was carried out using 60% ultrasonic power amplitude and 0.1 m hydrochloric acid for 15 min. separately, solid-liquid extraction was carried in an orbital shaker using 0.1 m hydrochloric acid at 70 °c for 2.5 h. uae with hydrochloric acid resulted in the highest concentration of laminarin, 5.82% a ... | 2015 | 26184235 |
| influence of general stress-response alternative sigma factors σ(s) (rpos) and σ(b) (sigb) on bacterial tolerance to the essential oils from origanum vulgare l. and rosmarinus officinalis l. and pulsed electric fields. | this study assessed the influence of general stress-response alternative sigma factors rpos (σ(s)) and sigb (σ(b)) on tolerance of escherichia coli (e. coli mg1655 and its isogenic mutant e. coli mg1655 δrpos) and listeria monocytogenes (l. monocytogenes egd-e and its isogenic mutant l. monocytogenes egd-e δsigb) to the essential oils (eos) from origanum vulgare l.-oregano (oveo) and rosmarinus officinalis l.-rosemary (roeo), as well as the changes in tolerance of parental and δrpos and δsigb mu ... | 2015 | 26159473 |
| inactivation of pathogenic bacteria inoculated onto a bacto™ agar model surface using tio2-uvc photocatalysis, uvc and chlorine treatments. | the aim of this study was to study inactivation of different pathogenic bacteria on agar model surface using tio2-uv photocatalysis (tuvp). | 2015 | 26099167 |
| cold plasma inactivation of internalised bacteria and biofilms for salmonella enterica serovar typhimurium, listeria monocytogenes and escherichia coli. | microbial biofilms and bacteria internalised in produce tissue may reduce the effectiveness of decontamination methods. in this study, the inactivation efficacy of in-package atmospheric cold plasma (acp) afterglow was investigated against salmonella typhimurium, listeria monocytogenes and escherichia coli in the forms of planktonic cultures, biofilms formed on lettuce and associated bacteria internalised in lettuce tissue. prepared lettuce broth (3%) was inoculated with bacteria resulting in a ... | 2015 | 26093991 |
| amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films. | silver nanoparticles (agnps) were synthesized using amino acids (tyrosine and tryptophan) as reducing and capping agents, and they were incorporated into the agar to prepare antimicrobial composite films. the agnps solutions exhibited characteristic absorption peak at 420 nm that showed a red shift to ∼434 nm after forming composite with agar. xrd data demonstrated the crystalline structure of agnps with dominant (111) facet. apparent surface color and transmittance of agar films were greatly in ... | 2015 | 26076636 |
| bacterial enteric infections among older adults in the united states: foodborne diseases active surveillance network, 1996-2012. | a growing segment of the population-adults aged ≥65 years-is more susceptible than younger adults to certain enteric (including foodborne) infections and experience more severe disease. | 2015 | 26067228 |
| impact of cell-free supernatant of lactic acid bacteria on putrescine and other polyamine formation by foodborne pathogens in ornithine decarboxylase broth. | conversion of ornithine to putrescine by salmonella paratyphi a, listeria monocytogenes, staphylococcus aureus, and escherichia coli was investigated in ornithine decarboxylase broth (odb) using cell-free supernatants (cfss) obtained from leuconostoc mesenterodies subsp. cremoris, pediococcus acidilactici, lactococcus lactis subsp. lactis, streptococcus thermophilus. two groups of cell-free supernatants (25 or 50%) and control (only odb) were prepared to investigate putrescine (put) and other po ... | 2015 | 26043863 |
| irrigation is significantly associated with an increased prevalence of listeria monocytogenes in produce production environments in new york state. | environmental (i.e., meteorological and landscape) factors and management practices can affect the prevalence of foodborne pathogens in produce production environments. this study was conducted to determine the prevalence of listeria monocytogenes, listeria species (including l. monocytogenes), salmonella, and shiga toxin-producing escherichia coli (stec) in produce production environments and to identify environmental factors and management practices associated with their isolation. ten produce ... | 2015 | 26038903 |
| epidemiology of bacteremia in previously healthy febrile infants: a follow-up study. | describe the etiology of bacteremia among a geographically diverse sample of previously well infants with fever admitted for general pediatric care and to characterize demographic and clinical characteristics of infants with bacteremia according to bacterial etiology. we hypothesized that the epidemiology of bacteremia in febrile infants from a geographically diverse cohort would show similar results to smaller or single-center cohorts previously reported. | 2015 | 26034160 |
| libyan thymus capitatus essential oil: antioxidant, antimicrobial, cytotoxic and colon pathogen adhesion-inhibition properties. | in the present work, the libyan wild-growing thymus capitatus essential oil (eo) was evaluated for its biological properties. | 2015 | 26033505 |
| detection of poultry meat specific bacteria using ftir spectroscopy and chemometrics. | ftir spectra of poultry meat specific bacteria viz. salmonella enteritidis, pseudomonas ludensis, listeria monocytogenes and escherichia coli were collected and investigated for identification of spectral windows capable of bacterial classification and quantification. two separate datasets obtained at different times were used in the study to check reproducibility of results. multivariate data analysis techniques viz. principal component analysis (pca), partial least-squares discriminant analysi ... | 2015 | 26028771 |
| a comparison of in-house real-time lamp assays with a commercial assay for the detection of pathogenic bacteria. | molecular detection of bacterial pathogens based on lamp methods is a faster and simpler approach than conventional culture methods. although different lamp-based methods for pathogenic bacterial detection are available, a systematic comparison of these different lamp assays has not been performed. in this paper, we compared 12 in-house real-time lamp assays with a commercialized kit (isothermal master mix) for the detection of listeria monocytogenes, salmonella spp, staphylococcus aureus, esche ... | 2015 | 26020698 |
| proteolytic and antimicrobial activity of lactic acid bacteria grown in goat milk. | we examined 62 strains and 21 trade starter cultures from the collection of lb bulgaricum plc for proteolytic and antimicrobial activity of lactic acid bacteria (lab) grown in goat milk. the aim of this study was to investigate the fermentation of caseins, α-lactalbumin and β-lactoglobulin by lab, using the o-phthaldialdehyde (opa) spectrophotometric assay and sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page). the proteolysis targeted mainly caseins, especially β-casein. whey ... | 2014 | 26019593 |
| listeria monocytogenes-carrying consortia in food industry. composition, subtyping and numerical characterisation of mono-species biofilm dynamics on stainless steel. | in order to find out how real listeria monocytogenes-carrying biofilms are in industrial settings, a total of 270 environmental samples belonging to work surfaces from fish (n = 123), meat (n = 75) and dairy industries (n = 72) were analysed in order to detect l. monocytogenes. 12 samples were positive for l. monocytogenes and a total of 18 different species were identified as accompanying microbiota in fish and meat industry. no l. monocytogenes was found in samples from dairy industry. molecul ... | 2015 | 26001376 |
| sublethal injury and virulence changes in listeria monocytogenes and listeria innocua treated with antimicrobials carvacrol and citral. | the aim of this study was to evaluate the effect of two antimicrobial substances, carvacrol and citral, on listeria monocytogenes and listeria innocua cells, as well as possible virulence changes in injured cells, using caenorhabditis elegans as a model test. the results indicated that the percentage of sublethal damage was higher in l. monocytogenes than in l. innocua. the results of the study carried out by using c. elegans indicated that c. elegans fed in a lawn of l. monocytogenes previously ... | 2015 | 25998809 |
| microbiological shelf life at different temperatures and fate of listeria monocytogenes and escherichia coli inoculated in unflavored and strawberry yogurts. | three different trials were performed on unflavored and strawberry yogurts produced in a small-scale dairy plant. in the first trial, the microbiological shelf life of the products was evaluated at 4, 8, and 20°c. at 4°c the product showed low total viable counts until the end of the trial (d 35=3.0±0.7 and 1.5±0.0 log cfu/g in unflavored and strawberry yogurt, respectively). the loads were lower in strawberry yogurt at 4°c compared with unflavored yogurt because of the antimicrobial activity ex ... | 2015 | 25981065 |
| microbiological quality of milk sold directly from producers to consumers. | the aim of this study was to determine the microbiological quality of raw cow milk from direct sale points. raw cow milk samples were collected from 5 randomly selected direct sale points for microbiological evaluation. the samples were analyzed to determine total aerobic bacterial count, somatic cell count (scc), counts of enterobacteriaceae, enterococcus, escherichia coli, and staphylococcus, and presence of salmonella, listeria monocytogenes, and inhibitory substances. the mean counts of tota ... | 2015 | 25958278 |
| the antibiotic activity and mechanisms of sugarcane (saccharum officinarum l.) bagasse extract against food-borne pathogens. | sugarcane bagasse contains natural compositions that can significantly inhibit food-borne pathogens growth. in the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol. the sugarcane bagasse extract showed bacteriostatic activity against the growth of staphylococcus aureus, listeria monocytogenes, escherichia coli and salomonella typhimurium. additionally, the sugarcane bagasse extract can increase the electri ... | 2015 | 25952848 |
| control of pathogens in biofilms on the surface of stainless steel by levulinic acid plus sodium dodecyl sulfate. | the efficacy of levulinic acid (lva) plus sodium dodecyl sulfate (sds) to remove or inactivate listeria monocytogenes, salmonella typhimurium, and shiga toxin-producing escherichia coli (stec) in biofilms on the surface of stainless steel coupons was evaluated. five- or six-strain mixtures (ca. 9.0 log cfu/ml) of the three pathogens were separately inoculated on stainless steel coupons. after incubation at 21 °c for 72 h, the coupons were treated for 10 min by different concentrations of lva plu ... | 2015 | 25950851 |
| evaluation of the antibacterial activity of the althaea officinalis l. leaf extract and its wound healing potency in the rat model of excision wound creation. | wound is defined simply as the disruption of the biochemical, cellular, and anatomic continuity of a tissue. plants and their extracts known as phytomedicine have immense potential for the management and treatment of wounds. | 2017 | 25949951 |
| ready-to-eat street-vended food as a potential vehicle of bacterial pathogens and antimicrobial resistance: an exploratory study in porto region, portugal. | the ready-to-eat street vending commerce, as street mobile food vendors, has grown exponentially worldwide, representing in some countries a significant proportion of food consumed by the urban population. however, the microbiological food safety hazards of mobile vending units in industrialized countries are scarcely evaluated. to assess the microbiological quality and safety of this type of food and try to achieve the connection of its contamination with hygienic conditions of food-handlers, w ... | 2015 | 25910073 |
| rapid detection of listeria monocytogenes in milk using confocal micro-raman spectroscopy and chemometric analysis. | listeria monocytogenes is a facultatively anaerobic, gram-positive, rod-shape foodborne bacterium causing invasive infection, listeriosis, in susceptible populations. rapid and high-throughput detection of this pathogen in dairy products is critical as milk and other dairy products have been implicated as food vehicles in several outbreaks. here we evaluated confocal micro-raman spectroscopy (785 nm laser) coupled with chemometric analysis to distinguish six closely related listeria species, inc ... | 2015 | 25863337 |
| assessment of microbial risk factors and impact of meteorological conditions during production of baby spinach in the southeast of spain. | there is a timely need to evaluate the effect agricultural factors and meteorological conditions on fresh produce contamination. this study evaluated those risk factors and described, for the first time, the distribution of indicator microorganisms (escherichia coli, enterococcus, coliforms, and enterobacteriaceae) and the prevalence of foodborne pathogens (enterohaemorrhagic e. coli, listeria monocytogenes and salmonella spp.) in baby spinach grown in the southeast of spain. a longitudinal stud ... | 2015 | 25846928 |
| effects of sub-lethal concentrations of thyme and oregano essential oils, carvacrol, thymol, citral and trans-2-hexenal on membrane fatty acid composition and volatile molecule profile of listeria monocytogenes, escherichia coli and salmonella enteritidis. | the aim of this work was to investigate the modifications of cell membrane fatty acid composition and volatile molecule profiles of listeria monocytogenes, salmonella enteritidis, escherichia coli, during growth in the presence of different sub-lethal concentrations of thyme and oregano essential oils as well as carvacrol, thymol, trans-2-hexenal and citral. the results evidenced that the tested molecules induced noticeable modifications of membrane fatty acid profiles and volatile compounds pro ... | 2015 | 25842326 |
| determining the potential link between irrigation water quality and the microbiological quality of onions by phenotypic and genotypic characterization of escherichia coli isolates. | the potential transfer of human pathogenic bacteria present in irrigation water onto fresh produce was investigated, because surface water sources used for irrigation purposes in south africa have increasingly been reported to be contaminated with enteric bacterial pathogens. a microbiological analysis was performed of a selected river in limpopo province, south africa, that is often contaminated with raw sewage from municipal sewage works and overhead irrigated onions produced on a commercial f ... | 2015 | 25836387 |
| comparison of the prevalences and diversities of listeria species and listeria monocytogenes in an urban and a rural agricultural watershed. | foods and related processing environments are commonly contaminated with the pathogenic listeria monocytogenes. to investigate potential environmental reservoirs of listeria spp. and l. monocytogenes, surface water and point source pollution samples from an urban and a rural municipal water supply watershed in nova scotia, canada, were examined over 18 months. presumptive listeria spp. were cultured from 72 and 35% of rural and urban water samples, respectively, with 24% of the positive samples ... | 2015 | 25819965 |
| commercially available rapid methods for detection of selected food-borne pathogens. | generally, the enumeration and isolation of food-borne pathogens is performed using culture-dependent methods. these methods are sensitive, inexpensive, and provide both qualitative and quantitative assessment of the microorganisms present in a sample, but these are time-consuming. for this reason, researchers are developing new techniques that allow detection of food pathogens in shorter period of time. this review identifies commercially available methods for rapid detection and quantification ... | 2016 | 25749054 |
| microbiological and shelf life characteristics of eviscerated and vacuum packed freshwater catfish (ompok pabda) during chill storage. | the effect of vacuum packaging technique on the shelflife of eviscerated pabda catfish (ompok pabda) during chill storage at 4 ± 2 °c was studied. the shelflife of pabda fish was predicted based on the cumulative observations of biochemical, microbiological and sensory parameters. there was no significant difference in the lipid quality parameters studied between the samples during the chill storage period, whereas, vacuum packaging significantly improved the sensory and microbiological quality. ... | 2015 | 25745210 |
| ceramide and sphingosine in pulmonary infections. | acid sphingomyelinase and ceramide have previously been shown to play a central role in infections with neisseria gonorrhoeae, staphylococcus aureus, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, escherichia coli, and mycobacterium avium. recent studies have extended the role of sphingolipids in bacterial infections and have demonstrated that ceramide and sphingosine are central to the defense of lungs against bacterial pathogens. ceramide accumulates in the airway epit ... | 2015 | 25720061 |
| prevalence of bacteria and absence of anisakid parasites in raw and prepared fish and seafood dishes in spanish restaurants. | this study evaluated the presence of bacteria and anisakid parasites in 45 samples of raw anchovies in vinegar, a dish widely eaten in spain, and in 227 samples of cooked fish and cephalopods served in spanish food service establishments. our analysis showed that, according to european and spanish regulation, 14 to 30% of the prepared fish and cephalopod dishes exceeded the maximum allowable level for mesophilic aerobic counts, and 10 to 40% of these samples exceeded the allowable levels for ent ... | 2015 | 25719890 |
| first two domains at the lp_1643 protein n terminus inhibit pathogen adhesion to porcine mucus in vitro. | gastrointestinal probiotics are important members of intestinal microflora in both healthy animals and human beings, and these bacteria may reduce the risk of infection caused by certain opportunistic pathogens through exclusive inhibition, competition, and displacement. the lp_1643 protein on the cell surface of lactobacillus plantarum wcfsi was assumed to possess a mucus-binding capability. this study aimed to determine if purified his-n2 protein exclusively inhibits pathogen adhesion to porci ... | 2015 | 25710153 |
| (aminophosphane)gold(i) and silver(i) complexes as antibacterial agents. | this manuscript describes the synthesis of new au(i) and ag(i) complexes with aminophosphane ligands and a study of their antibacterial activity against gram-negative salmonella enterica serovar typhimurium and escherichia coli and gram-positive listeria monocytogenes and staphylococcus aureus. the bactericidal assays revealed the effectiveness of these compounds on paradigm gram-negative and gram-positive pathogens, showing a moderate antimicrobial activity, comparable with the antibiotics of r ... | 2015 | 25706322 |
| the expression of propionicin plg-1 gene (plg-1) by lactic starters. | propionicin plg-1 is a bacteriocin produced by propionibacterium thoenii p127. such bacteriocin inhibits wide range of food-borne pathogens such as pathogenic escherichia coli, pseudomonas aeruginosa, vibrio parahaemolyticus, yersinia enterocolitica and a strain of corynebacterium sp. in the present study, plg-1 gene expressing propionicin plg-1 was isolated, sequenced for the first time and the resulting sequence was analysed using several web-based bioinformatics programs. the pcr product cont ... | 2015 | 25684078 |
| a 3-year hygiene and safety monitoring of a meat processing plant which uses raw materials of global origin. | a systematic approach in monitoring the hygiene of a meat processing plant using classical microbiological analyses combined with molecular characterization tools may assist in the safety of the final products. this study aimed: (i) to evaluate the total hygiene level and, (ii) to monitor and characterize the occurrence and spread of salmonella spp. and listeria monocytogenes in the environment and the final products of a meat industry that processes meat of global origin. in total, 2541 samples ... | 2015 | 25600954 |
| analytical bioconjugates, aptamers, enable specific quantitative detection of listeria monocytogenes. | as a major human pathogen in the listeria genus, listeria monocytogenes causes the bacterial disease listeriosis, which is a serious infection caused by eating food contaminated with the bacteria. we have developed an aptamer-based sandwich assay (absa) platform that demonstrates a promising potential for use in pathogen detection using aptamers as analytical bioconjugates. the whole-bacteria selex (wb-selex) strategy was adopted to generate aptamers with high affinity and specificity against li ... | 2015 | 25590973 |
| storage stability and antibacterial activity of eugenol nanoliposomes prepared by an ethanol injection-dynamic high-pressure microfluidization method. | eugenol is a major phenolic component with diverse biological activities. however, it is difficult to formulate into an aqueous solution due to poor water solubility, and this limits its application. in the present study, eugenol nanoliposomes (en) were prepared by combining the ethanol injection method with the dynamic high-pressure microfluidization method. good physicochemical characterizations of en were obtained. the successful encapsulation of eugenol in nanoliposomes was confirmed by four ... | 2015 | 25581174 |
| the effect of surface properties of polycrystalline, single phase metal coatings on bacterial retention. | in the food industry microbial contamination of surfaces can result in product spoilage which may lead to potential health problems of the consumer. surface properties can have a substantial effect on microbial retention. the surface characteristics of chemically different coatings (cu, ti, mo, ag, fe) were defined using white light profilometry (micro-topography and surface features), atomic force microscopy (nano-topography) and physicochemical measurements. the ag coating had the greatest top ... | 2015 | 25576986 |
| intradermal administration of the type ii heat-labile enterotoxins lt-iib and lt-iic of enterotoxigenic escherichia coli enhances humoral and cd8+ t cell immunity to a co-administered antigen. | vaccinations are extremely effective at combating infectious diseases. many conserved antigen (ag) targets, however, are poorly immunogenic. protein subunit vaccines frequently elicit only humoral immune responses and fail to confer protection against serious intracellular pathogens. these barriers to vaccine development are often overcome by the use of appropriate adjuvants. heat-labile enterotoxins (hlt) produced by enterotoxigenic strains of escherichia coli are potent adjuvants when administ ... | 2014 | 25536061 |
| the microbiological quality of ready-to-eat salads in turkey: a focus on salmonella spp. and listeria monocytogenes. | the microbiological safety of ready-to-eat (rte) foods is of special concern as they are not exposed to further processing before consumption. in the present study, listeria monocytogenes and salmonella spp. were isolated from 15(6%) and 21(8%) samples respectively out of 261 rte foods commercialized in turkey. escherichia coli was present in 10(4%) samples analyzed. psychrotrophic aerobic populations >6logcfu/g were found in 36 (14%) of the samples, while total coliforms were detected in 155 (5 ... | 2015 | 25528536 |
| antimicrobial activity of hop extracts against foodborne pathogens for meat applications. | the objective of this study was the fundamental investigation of the antimicrobial efficiency of various hop extracts against selected foodborne pathogens in vitro, as well as their activity against listeria monocytogenes in a model meat marinade and on marinated pork tenderloins. | 2015 | 25494620 |
| inactivation kinetics of foodborne pathogens by uv-c radiation and its subsequent growth in fresh-cut kailan-hybrid broccoli. | the inactivation of escherichia coli, s. enteritidis and listeria monocytogenes after uv-c radiation with 0, 2.5, 5, 7.5, 10 and 15 kj uv-c m(-2) on fresh-cut kailan-hybrid broccoli was explored. inactivation did not follow linear kinetics. hence, it was modelled by using the weibull distribution function, obtaining adjusted r(2) values higher than 94%, indicative of the accuracy of the model to the experimental data. the uv-c doses needed to reduce 1 log cycle the e. coli, s. enteritidis and l. ... | 2015 | 25475295 |
| evaluation of pathogen inactivation on sliced cheese induced by encapsulated atmospheric pressure dielectric barrier discharge plasma. | pathogen inactivation induced by atmospheric pressure dielectric barrier discharge (dbd) (250 w, 15 khz, air discharge) produced in a rectangular plastic container and the effect of post-treatment storage time on inactivation were evaluated using agar plates and cheese slices. when agar plates were treated with plasma, populations of escherichia coli, salmonella typhimurium, and listeria monocytogenes showed 3.57, 6.69, and 6.53 decimal reductions at 60 s, 45 s, and 7 min, respectively. when the ... | 2015 | 25475265 |
| the influence of the cell free solution of lactic acid bacteria on tyramine production by food borne-pathogens in tyrosine decarboxylase broth. | the function of cell-free solutions (cfss) of lactic acid bacteria (lab) on tyramine and other biogenic amine production by different food borne-pathogens (fbps) was investigated in tyrosine decarboxylase broth (tdb) using hplc. cell free solutions were prepared from four lab strains. two different concentrations which were 50% (5 ml cfs+5 ml medium/1:1) and 25% (2.5 ml cfs+7.5 ml medium/1:3) cfs and the control without cfs were prepared. both concentration of cfs of streptococcus thermophilus a ... | 2015 | 25465993 |
| prevalence and predictors of bacterial meningitis in young infants with fever without a source. | classical criteria differ when performing cerebrospinal fluid (csf) analysis in infants younger than 90 days with fever without a source (fws). our objectives were to analyze the prevalence and microbiology of bacterial meningitis in this group and its prevalence in relation to clinical and laboratory risk factors. | 2015 | 25461476 |
| antimicrobial effects of short chained imidazolium-based ionic liquids—influence of anion chaotropicity. | ionic liquids (ils), a new solvent class composed solely of ions, have already found their way into numerous chemical and biochemical applications. due to their unique properties and wide application range, research utilizing this new technology for biotechnological applications is steadily increasing. however, progress is hampered by lack of toxicological data, especially concerning il anions and their general underlying toxicity mechanisms. the present study investigated for the first time the ... | 2015 | 25450920 |
| brazilian fruit pulps as functional foods and additives: evaluation of bioactive compounds. | eight tropical fruit pulps from brazil were simultaneously characterised in terms of their antioxidant and antimicrobial properties. antioxidant activity was screened by dpph radical scavenging activity (126-3987 mg te/100g dw) and ferric reduction activity power (368-20819 mg aae/100g dw), and complemented with total phenolic content (329-12466 mg gae/100g dw) and total flavonoid content measurements (46-672 mg ee /100g dw), whereas antimicrobial activity was tested against the most frequently ... | 2015 | 25442579 |
| application of dna aptamers and quantum dots to lateral flow test strips for detection of foodborne pathogens with improved sensitivity versus colloidal gold. | preliminary studies aimed at improving the sensitivity of foodborne pathogen detection via lateral flow (lf) test strips by use of high affinity dna aptamers for capture and reporter functions when coupled to red-emitting quantum dots (qdot 655) are reported. a variety of dna aptamers developed against escherichia coli, listeria monocytogenes, and salmonella enterica were paired in capture and reporter combinations to determine which yielded the strongest detection of their cognate bacteria usin ... | 2014 | 25437803 |
| microbiological profile and incidence of salmonella and listeria monocytogenes on hydroponic bell peppers and greenhouse cultivation environment. | the aim of this study was to generate information regarding the microbiological profile, including salmonella and listeria monocytogenes incidence, of hydroponically grown bell peppers and materials associated with their production in greenhouses located in mexico. samples of coconut fiber (24), knives (30), drippers (20), conveyor belts (161), pepper transportation wagons (30), air (178), water (16), nutrient solution for plant irrigation (78), and bell pepper fruits (528) were collected during ... | 2014 | 25364924 |
| detection and identification of six foodborne bacteria by two-tube multiplex real time pcr and melting curve analysis. | this study is aimed to develop a two-tube melting curve-based multiplex real time pcr assay (mcmrt-pcr) for the simultaneous detection of six common foodborne pathogenic bacteria (diarrhoeagenic escherichia coli, salmonella, and shigella in tube 1, staphylococcus aureus, vibrio parahaemolyticus, and listeria monocytogenes in tube 2). | 2014 | 25341812 |
| treatment strategies for central nervous system infections: an update. | central nervous system infection continues to be an important cause of mortality and morbidity worldwide. our incomplete knowledge on the pathogenesis of how meningitis-causing pathogens cause cns infection and emergence of antimicrobial resistance has contributed to the mortality and morbidity. an early empiric antibiotic treatment is critical for the management of patients with bacterial meningitis, but early recognition of bacterial meningitis continues to be a challenge. | 2015 | 25328149 |
| assessment of the contamination potentials of some foodborne bacteria in biofilms for food products. | to assess biofilms formed by different bacterial strains on glass slides, and changes in biofilm mass and biofilm-associated cell populations after brief contacts between biofilms and either media agar or food products. | 2014 | 25312128 |
| effect of gamma radiation on the reduction of salmonella strains, listeria monocytogenes, and shiga toxin-producing escherichia coli and sensory evaluation of minimally processed spinach (tetragonia expansa). | this study evaluated the effects of irradiation on the reduction of shiga toxin-producing escherichia coli (stec), salmonella strains, and listeria monocytogenes, as well as on the sensory characteristics of minimally processed spinach. spinach samples were inoculated with a cocktail of three strains each of stec, salmonella strains, and l. monocytogenes, separately, and were exposed to gamma radiation doses of 0, 0.2, 0.4, 0.6, 0.8, and 1.0 kgy. samples that were exposed to 0.0, 1.0, and 1.5 kg ... | 2014 | 25285495 |