| potentially probiotic and bioprotective lactic acid bacteria starter cultures antagonise the listeria monocytogenes adhesion to ht29 colonocyte-like cells. | the capability of five lactic acid bacteria (lab) to counteract the adhesion of listeria monocytogenes to the epithelial intestinal cell line ht29 was studied. the highest adhesion ability to ht29 was achieved by the intestinal strain lactobacillus rhamnosus ctc1679, followed by the meat-derived strains lactobacillus sakei ctc494 and enterococcus faecium ctc8005. surprisingly, the meat strains showed significantly better adhesion to ht29 than two faecal isolates of lactobacillus casei and even s ... | 2015 | 25488261 |
| an antilisterial bacteriocin bacfl31 produced by enterococcus faecium fl31 with a novel structure containing hydroxyproline residues. | a new bacterium called fl31, which was selected for its antimicrobial activity against the pathogenic bacterium listeria monocytogenes, was identified as enterococcus faecium and noted to produce an antibacterial proteinaceous substance (bacfl31). the active peptide from the cell-free supernatant of e. faecium fl31 was purified in four steps and the results revealed a single band with an estimated molecular mass of approximately 3.5 kda. the n-terminal amino acid sequence of the purified bacfl31 ... | 2014 | 24583094 |
| enterocin hz produced by a wild enterococcus faecium strain isolated from a traditional, starter-free pickled cheese. | bacteriogenic enterococcus faecium hz was identified by using biochemical (strep-api 20, api-50 chl, fatty acid profile) and 16s rrna analysis (99·99 %). ent. faecium hz was sensitive to clinically important antibiotics such as vancomycin, and did not have gelatinase and haemolysis activities. enterocin hz, a bacteriocin from ent. faecium hz, was sensitive to papain and tyripsin, but resistant to pepsin, lipase, catalase, α-amylase, organic solvents, detergents, ß-mercaptoethanol, and heat treat ... | 2014 | 24559899 |
| antibacterial efficacy of nisin, pediocin 34 and enterocin fh99 against l. monocytogenes, e. faecium and e. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants. | the bacteriocin susceptibility of listeria monocytogenes mtcc 657, enterococcus faecium dsmz 20477, e. faecium vre, and e. faecalis atcc 29212 and their corresponding bacteriocin resistant variants was assessed. the single and combined effect of nisin and pediocin 34 and enterocin fh99 bacteriocins produced by pediococcus pentosaceus 34, and e. faecium fh99, respectively, was determined. pediocin34 proved to be more effective in inhibiting l. monocytogenes mtcc 657. a greater antibacterial effec ... | 2014 | 24493880 |
| in vitro study of beneficial properties and safety of lactic acid bacteria isolated from portuguese fermented meat products. | many lactic acid bacteria produce bacteriocins with a rather broad spectrum of inhibition, which could offer potential applications in food preservation. bacteriocin production by starter cultures may bring advantage to these strains in competitive interactions with pathogenic bacteria from the food matrix. the objective of this study was to determine the safety of beneficial strains (lactobacillus plantarum st202ch and st216ch, enterococcus faecium st211ch, and lactobacillus sakei st22ch, st153 ... | 2014 | 24463204 |
| bacteriocinogenic potential and safety evaluation of non-starter enterococcus faecium strains isolated from home made white brine cheese. | four lab strains, isolated from bulgarian home made white brine cheese, were selected for their effective inhibition against listeria monocytogenes. according to their biochemical and physiological characteristics, the strains were classified as members of enterococcus genus, and then identified as enterococcus faecium by 16s rdna sequencing. their bacteriocin production and inhibitory spectrum were evaluated together with the occurrence of several bacteriocin genes (enta, entb, entp, entl50b). ... | 2014 | 24290647 |
| enterocin tw21, a novel bacteriocin from dochi-isolated enterococcus faecium d081821. | purification and characterization of a novel bacteriocin produced by strain enterococcus faecium d081821. | 2013 | 23725102 |
| partial characterization of bacteriocins produced by two new enterococcus faecium isolated from human intestine. | this study aimed at characterizing two novel bacteriocin-producing enterococcal strains isolated from human intestine. a total of 200 lactic acid bacteria were isolated from a woman stool sample. two of them were selected for characterization due to their high antimicrobial activity against five strains of listeria monocytogenes. the selected bacteria were identified as two different strains of enterococcus faecium and designated mt 104 and mt 162. the bacteriocins produced by mt 104 and mt 162 ... | 2013 | 26782736 |
| isolation and identification of bacteriocinogenic strain of lactobacillus plantarum with potential beneficial properties from donkey milk. | the goal of this study was to isolate and characterize a lactic acid bacteria (lab) from donkey milk with potential beneficial properties. | 2013 | 23489977 |
| synthesis and functional characterization of antibiofilm exopolysaccharide produced by enterococcus faecium mc13 isolated from the gut of fish. | the synthesis and functional characterization of an antibiofilm exopolysaccharide (eps) from a probiotic enterococcus faecium mc13 were investigated. the temperature of 35 °c, ph of 6.5, and salinity of 1-2% were found to be optimum for eps production. the sucrose (30 g l⁻¹) and yeast extract (20 g l⁻¹) acted as suitable carbon and nitrogen sources, respectively, which strongly influenced eps production with yield of 11.33 and 11.91 g l⁻¹. based on the thin layer chromatography, eps of e. faeciu ... | 2013 | 23296803 |
| screening and characterization of lab-produced bacteriocin-like substances from the intestine of grey mullet (mugil cephalus l.) as potential biocontrol agents in aquaculture. | to isolate and characterize bacteriocin-like inhibitory substance (blis)-producing lactic acid bacteria from the intestine of grey mullet. | 2013 | 23075097 |
| immunomodulatory properties of enterococcus faecium jws 833 isolated from duck intestinal tract and suppression of listeria monocytogenes infection. | the aim of this study was to evaluate the immunomodulatory properties of enterococcus faecium jws 833 (jws 833) isolated from duck intestine and compare them to those of lactobacillus rhamnosus gg (lgg), a proven immunity-enhancing probiotic. to investigate the immune-enhancing properties of jws 833, production of nitric oxide (no) and cytokines was measured in mouse peritoneal macrophages. in addition, a listeria monocytogenes challenge model was used in the assessment. it was found that heat-k ... | 2012 | 22709265 |
| characterization of a heat stable anti-listerial bacteriocin produced by vancomycin sensitive enterococcus faecium isolated from idli batter. | lactic acid bacteria (lab) are known to produce various types of bacteriocins, ribosomally synthesized polypeptides, which have antibacterial spectrum against many food borne pathogens. listeria monocytogenes, a pathogenic bacterium, is of particular concern to the food industry because of its ability to grow even at refrigeration temperatures and its tolerance to preservative agents. some of the bacteriocins of lab are known to have anti-listerial property. in the present study, the bacteriocin ... | 2010 | 23100837 |
| phylogenetic profiles of in-house microflora in drains at a food production facility: comparison and biocontrol implications of listeria-positive and -negative bacterial populations. | listeria species experience complex interactions with other microorganisms, which may promote growth and colonization of the organism in local environments or negatively affect them. this study investigated the microbial community at a food production facility, examining interactions between listeria and the associated microbiome. listeria species can be transferred between zones in the production environment by individuals or equipment, and drains may act as a reservoir for the organism, reflec ... | 2014 | 24657862 |
| novel bacteriocinogenic enterococcus hirae and pediococcus pentosaceus strains with antilisterial activity isolated from brazilian artisanal cheese. | we isolated and characterized bacteriocin producers enterococcus hirae st57acc and pediococcus pentosaceus st65acc from raw milk artisanal cheeses. their bacteriocins were tolerant to temperatures from 4°c to 100°c and under sterilization conditions (121°c for 15 min). additionally, the tested bacteriocins remained active after being exposed to ph 2.0 to 10.0 for 2 h. the activity of the bacteriocins was affected by proteolytic enzymes but remained stable after treatment with edta, sodium dodecy ... | 2017 | 28189328 |
| influence of pdo ragusano cheese biofilm microbiota on flavour compounds formation. | the objectives of the present study were to characterize the biofilm microbiota of 11 different farms (from a to k), producing pdo ragusano cheese, and to investigate on its ability to generate volatile organic compounds (vocs) in milk samples inoculated with biofilm and incubated under ragusano cheese making conditions. the biofilms were subjected to plate counting and pcr/t/dgge analysis and the vocs generated in incubated milk samples were evaluated through smartnose, gc/o, and gc/ms. strepto ... | 2017 | 27697162 |
| biochemical properties and mechanism of action of enterocin ld3 purified from enterococcus hirae ld3. | enterocin ld3 was purified using activity-guided multistep chromatography techniques such as cation-exchange and gel-filtration chromatography. the preparation's purity was tested using reverse-phase ultra-performance liquid chromatography. the specific activity was tested to be 187.5 au µg(-1) with 13-fold purification. purified enterocin ld3 was heat stable up to 121 °c (at 15 psi pressure) and ph 2-6. the activity was lost in the presence of papain, reduced by proteinase k, pepsin and trypsin ... | 2016 | 27145777 |
| antimicrobial characterization and safety aspects of the bacteriocinogenic enterococcus hirae f420 isolated from moroccan raw goat milk. | the f420 strain, isolated from raw goat milk and identified as enterococcus hirae, was selected because of its strong activity against gram-positive bacteria, including listeria monocytogenes. interestingly, the f420 strain lacks the virulence genes and decarboxylase activity of histidine, lysine, and ornithine, and it is susceptible to 11 of 14 tested antibiotics, including vancomycin. the antimicrobial compounds produced by e. hirae f420 strain showed high resistance to heat treatment and to a ... | 2012 | 22471967 |
| enterococcus mundtii st4sa and lactobacillus plantarum 423 excludes listeria monocytogenes from the git, as shown by bioluminescent studies in mice. | listeria monocytogenes is an opportunistic food-borne pathogen and is life-threatening to individuals with a weakened immune system. the aim of this study was to determine if lactobacillus plantarum 423 and enterococcus mundtii st4sa could prevent colonisation of l. monocytogenes in the gastro-intestinal tract (git). mice were gavaged with l. plantarum 423, e. mundtii st4sa, and a combination of the two strains, for 6 consecutive days and orally infected with a bioluminescent strain of l. monocy ... | 2016 | 26689230 |
| a study on prevalence of microbial contamination on the surface of raw salad vegetables. | the present work evaluates the microbiological quality of raw salad vegetables (rsv) consumed in dhanbad city, india. a total of 480 samples of 8 different raw salad vegetables from local market were examined for overall microbial quality in terms of aerobic mesophilic, psychrotrophic counts, yeast, mould and total coliform levels. e. coli o157:h7, listeria monocytogenes and salmonella sp. were detected by real-time polymerase chain reaction (qpcr) subsequent to isolation. results showed that al ... | 2017 | 28391475 |
| finding novel antibiotic substances from medicinal plants - antimicrobial properties of nigella sativa directed against multidrug-resistant bacteria. | the progressive rise in multidrug-resistant (mdr) bacterial strains poses serious problems in the treatment of infectious diseases. while the number of newly developed antimicrobial compounds has greatly fallen, the resistance of pathogens against commonly prescribed drugs is further increasing. this rise in resistance illustrates the need for developing novel therapeutic and preventive antimicrobial options. the medicinal herb nigella sativa and its derivatives constitute promising candidates. ... | 2017 | 28386474 |
| microbiological and sensory quality of fresh ready-to-eat artichoke hearts packaged under modified atmosphere. | in recent years the sales of minimally processed vegetables have grown exponentially as a result of changes in consumer habits. the availability of artichoke buds as a ready-to-eat product would be, therefore, highly advantageous. however, minimally processed artichoke hearts are difficult to preserve because of their rapid browning and the proliferation of naturally occurring microorganisms. we developed artichoke hearts prepared as ready-to-eat products that maintain the characteristics of the ... | 2017 | 28358262 |
| disinfection and removal of human pathogenic bacteria in arctic waste stabilization ponds. | wastewater stabilization ponds (wsps) are commonly used to treat municipal wastewater in arctic canada. the biological treatment in the wsps is strongly influenced by climatic conditions. currently, there is limited information about the removal of fecal and pathogenic bacteria during the short cool summer treatment season. with relevance to public health, the objectives of this paper were to determine if treatment in arctic wsps resulted in the disinfection (i.e., removal of fecal indicator bac ... | 2017 | 28353112 |
| production of reuterin in a fermented milk product by lactobacillus reuteri: inhibition of pathogens, spoilage microorganisms, and lactic acid bacteria. | we assessed the antimicrobial activity of reuterin produced in vitro in glycerol aqueous solutions in situ by lactobacillus reuteri atcc 53608 as part of a fermented milk product against starter (lactobacillus delbrueckii ssp. bulgaricus and streptococcus thermophilus), spoilage (penicillium expansum), pathogenic (staphylococcus aureus, salmonella enterica ssp. enterica, and listeria monocytogenes), and pathogen surrogate (escherichia coli dh5α) microorganisms. we also assayed the influence of c ... | 2017 | 28342608 |
| antibacterial activity of phenyllactic acid against listeria monocytogenes and escherichia coli by dual mechanisms. | phenyllactic acid (pla), a phenolic acid phytochemical, is considered to be a promising candidate for use as a chemical preservative due to its broad antimicrobial activity. the antibacterial target of pla has rarely been reported, thus investigations were performed to elucidate the antibacterial mechanism of pla against listeria monocytogenes and escherichia coli. flow cytometry analysis stained with propidium iodide (pi) demonstrated that pla could damage the membrane integrity of l. monocytog ... | 2017 | 28317760 |
| prevalence of foodborne pathogens in food from selected african countries - a meta-analysis. | food safety information in the african region is insufficient and fragmented due to lack of surveillance, documentation and reporting, thereby resulting in inefficient utilization of resources, duplication of activities, and lack of synergy among the countries of the region. this paper reviews the prevalence of foodborne pathogens in seven african countries (benin, botswana, ghana, kenya, nigeria, sudan and uganda) from papers in regional or international journals published between january 2000 ... | 2017 | 28271855 |
| facile approach for large-scale production of metal and metal oxide nanoparticles and preparation of antibacterial cotton pads. | metallic nanoparticles such as zinc oxide (znonps), copper oxide (cuonps), and silver (agnps) were synthesized in gram scale using green methods. the antibacterial cotton fibers/nanoparticles (cotton fibers/nps) composite pads were prepared, and nanoparticle binding/release tests were performed. all the nps were crystalline and showed characteristic xrd diffraction peaks and showed the characteristic ftir bands of the respective nanoparticles. all the nps showed strong antimicrobial activity aga ... | 2017 | 28267490 |
| one-step preparation of banana powder/silver nanoparticles composite films. | silver nanoparticles (ag-nps) were synthesized using banana powder as a reducing and stabilizing agent, and banana/ag-nps composite films with different concentration of ag-nps were prepared simultaneously. the composite films were yellowish brown and exhibited characteristic plasmon resonance peak of ag-nps at 430 nm. the optical, mechanical, water vapor barrier, thermal stability, and antimicrobial properties of the composite films were greatly influenced by the concentration of ag-nps. the co ... | 2017 | 28242949 |
| evaluation of electrolytically-generated hypochlorous acid ('electrolyzed water') for sanitation of meat and meat-contact surfaces. | 'electrolyzed water' generators are readily available in the food industry as a renewable source of hypochlorous acid that eliminates the need for workers to handle hazardous hypochlorite concentrates. we applied electrolyzed water (ew) directly to multi-strain cocktails of listeria monocytogenes, e. coli o157:h7, and salmonella sp. at 250 ppm free available chlorine (fac) and achieved greater than 6-log reductions in 2 min. lower ew values were examined as antimicrobial interventions for fresh ... | 2016 | 28231137 |
| structural characterization and in vitro fermentation of β-mannooligosaccharides produced from locust bean gum by gh-26 endo-β-1,4-mannanase (manb-1601). | size exclusion chromatography of β-mannooligosaccharides (β-mos) mixtures, obtained from manb-1601 hydrolysis of locust bean gum, resulted in separation of oligosaccharides with various degrees of polymerization (dp 2, 3, and 5). the oligosaccharides were structurally [esi-ms, ftir, xrd, tga, and nmr ((1)h and (13)c)] and functionally (in vitro fermentation) characterized. dp2 oligosaccharide was composed of two species, (a) mannopyranose β-1,4 mannopyranose and (b) α-1,6-galactosyl-mannopyranos ... | 2017 | 28225615 |
| enrichment, amplification, and sequence-based typing of salmonella enterica and other foodborne pathogens. | detection of salmonella enterica in foods typically involves microbiological enrichment, molecular-based assay, and subsequent isolation and identification of a pure culture. this is ideally followed by strain typing, which provides information critical to the investigation of outbreaks and the attribution of their sources. pulsed-field gel electrophoresis is the "gold standard" for s. enterica strain typing, but its limitations have encouraged the search for alternative methods, including whole ... | 2017 | 28221883 |
| microbial performance of food safety control and assurance activities in a fresh produce processing sector measured using a microbial assessment scheme and statistical modeling. | current approaches such as inspections, audits, and end product testing cannot detect the distribution and dynamics of microbial contamination. despite the implementation of current food safety management systems, foodborne outbreaks linked to fresh produce continue to be reported. a microbial assessment scheme and statistical modeling were used to systematically assess the microbial performance of core control and assurance activities in five kenyan fresh produce processing and export companies ... | 2017 | 28221882 |
| microbiological quality of pastrami and associated surfaces at the point of sale in kayseri, turkey. | the aim of this study is to trace the possible relations between the hygienic status of slicing utensils and the microbiological quality of pastrami. | 2017 | 28216007 |
| the effects of atmospheric pressure cold plasma treatment on microbiological, physical-chemical and sensory characteristics of vacuum packaged beef loin. | effects on vacuum packaged and non-packaged beef longissimus samples exposed to atmospheric cold plasma (acp) generated at different powers were studied over a 10day period of vacuum-, and a subsequent 3day period of aerobic storage. exposure of non-covered beef samples under high power acp conditions resulted in increased a*, b*, chroma and hue values, but acp treatment of packaged loins did not impact colour (l*, a*, b*, chroma, hue), lipid peroxidation, sarcoplasmic protein denaturation, nitr ... | 2017 | 28214695 |
| influence of electro-activated solutions of weak organic acid salts on microbial quality and overall appearance of blueberries during storage. | the aim of this work was to study the potential of diluted electro-activated solutions of weak organic acid salts (potassium acetate, potassium citrate and calcium lactate) to extend the shelf life of blueberries during post-harvest storage. the sanitizing capacity of these solutions was studied against pathogenic bacteria listeria monocytogenes and e. coli o157:h7 as well as phytopathogenic fungi a. alternata, f. oxysporum and b. cinerea. the results showed that a 5-min treatment of inoculated ... | 2017 | 28213035 |
| inactivation of salmonella spp., pathogenic escherichia coli, staphylococcus spp., or listeria monocytogenes in chicken purge or skin using a 405-nm led array. | raw poultry are sometimes contaminated with foodborne pathogens, which can lead to illness in humans. in recent years research has focused on a variety of light technologies to decontaminate food and food contact surfaces during meat and poultry processing. in this study we evaluated the ability of 405-nm light generated from an led array to inactivate multi-isolate cocktails of either salmonella spp., pathogenic escherichia coli, staphylococcus spp., or listeria monocytogenes suspended in chick ... | 2017 | 28213017 |
| prevalence of listeria monocytogenes in retail lightly pickled vegetables and its successful control at processing plants. | incidences of food poisoning traced to nonanimal food products have been increasingly reported. one of these was a recent large outbreak of shiga toxin-producing escherichia coli (stec) o157 infection from the consumption of lightly pickled vegetables, indicating the necessity of imposing hygienic controls during manufacturing. however, little is known about the bacterial contamination levels in these minimally processed vegetables. here we examined the prevalence of stec, salmonella spp., and l ... | 2017 | 28207308 |
| evaluation of the effect of radiofrequency radiation emitted from wi-fi router and mobile phone simulator on the antibacterial susceptibility of pathogenic bacteria listeria monocytogenes and escherichia coli. | mobile phones and wi-fi radiofrequency radiation are among the main sources of the exposure of the general population to radiofrequency electromagnetic fields (rf-emf). previous studies have shown that exposure of microorganisms to rf-emfs can be associated with a wide spectrum of changes ranged from the modified bacterial growth to the alterations of the pattern of antibiotic resistance. our laboratory at the nonionizing department of the ionizing and non-ionizing radiation protection research ... | 2017 | 28203122 |
| attachment of 13 types of foodborne bacteria to jalapeño and serrano peppers and antibacterial effect of roselle calyx extracts, sodium hypochlorite, colloidal silver, and acetic acid against these foodborne bacteria on peppers. | chili peppers are a very important crop in mexico. however, these peppers have been associated with salmonella infection outbreaks in the united states, and salmonella and diarrheagenic escherichia coli pathotypes have been isolated from jalapeño and serrano peppers in mexico. to decrease microbial contamination of fruits and vegetables, chemical agents are commonly used; however, chemical agents used to eliminate pathogenic bacteria on vegetables have a limited antimicrobial effect. roselle ( h ... | 2017 | 28199144 |
| anti-pathogenic and probiotic attributes of lactobacillus salivarius and lactobacillus plantarum strains isolated from feces of algerian infants and adults. | sixty-seven (67) lactic acid bacteria (lab) isolates belonging to lactobacillus genus were isolated from human feces and tested for their auto-aggregation and cell surface hydrophobicity in order to establish their adhesion capabilities, a prerequisite for probiotic selection. strains with the upmost auto-aggregation and cell surface hydrophobicity scores were identified by maldi-tof spectrometry and 16s rdna sequencing as lactobacillus plantarum (p25lb1 and p98lb1) and lactobacillus salivarius ... | 2017 | 28093322 |
| microbiological and chemico-physical shelf-life and panel test to evaluate acceptability of liver mortadella. | this study aimed to evaluate the shelf life of sliced cooked liver mortadella packaged in map (70-85% n2, 15-30% co2) and stored in refrigeration (4°c) or slight thermal abuse (8°c) for up to 49 days (declared best before date 45 days). the proximate composition, aw nitrites and nacl content were determined at t0. weekly, samples were submitted to microbiological [total viable count (tvc), lactic acid bacteria (lab), enterobacteriaceae, escherichia coli, pseudomonas spp., coagulase positive stap ... | 2016 | 28058249 |
| presence of foodborne pathogens, extended-spectrum β-lactamase -producing enterobacteriaceae, and methicillin-resistant staphylococcus aureus in slaughtered reindeer in northern finland and norway. | various food-producing animals were recognized in recent years as healthy carriers of bacterial pathogens causing human illness. in northern fennoscandia, the husbandry of semi-domesticated reindeer (rangifer tarandus tarandus) is a traditional livelihood and meat is the main product. this study determined the presence of selected foodborne pathogens, methicillin-resistant staphylococcus aureus (mrsa), and extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae in healthy semi-domestic ... | 2017 | 28049493 |
| cord blood acute phase reactants predict early onset neonatal sepsis in preterm infants. | early onset sepsis (eos) is a major cause of morbidity and mortality in preterm infants, yet diagnosis remains inadequate resulting in missed cases or prolonged empiric antibiotics with adverse consequences. evaluation of acute phase reactant (apr) biomarkers in umbilical cord blood at birth may improve eos detection in preterm infants with intrauterine infection. | 2017 | 28045978 |
| inhibition of crispr-cas9 with bacteriophage proteins. | bacterial crispr-cas systems utilize sequence-specific rna-guided nucleases to defend against bacteriophage infection. as a countermeasure, numerous phages are known that produce proteins to block the function of class 1 crispr-cas systems. however, currently no proteins are known to inhibit the widely used class 2 crispr-cas9 system. to find these inhibitors, we searched cas9-containing bacterial genomes for the co-existence of a crispr spacer and its target, a potential indicator for crispr in ... | 2017 | 28041849 |
| occurrence and characterization of food-borne pathogens isolated from fruit, vegetables and sprouts retailed in the czech republic. | food of non-animal origin is a major component of the human diet and has been considered to pose a low risk from the point of view of bacteriological safety. however, an increase in the number of outbreaks of illness caused by such pathogens and linked to the consumption of fresh fruit and vegetables have been reported from around the world recently. salmonella spp., stec (shiga toxin producing escherichia coli) and listeria monocytogenes are among the most frequently identified agents. addition ... | 2017 | 28040162 |
| evaluation of molecular typing of foodborne pathogens in european reference laboratories from 2012 to 2013. | in 2012, the european centre for disease prevention and control (ecdc) initiated external quality assessment (eqa) schemes for molecular typing including the national public health reference laboratories in europe. the overall aim for these eqa schemes was to enhance the european surveillance of food-borne pathogens by evaluating and improving the quality and comparability of molecular typing. the eqas were organised by statens serum institut (ssi) and included salmonella enterica subsp. enteric ... | 2016 | 28006653 |
| evaluation of hygienic quality and labelling of fish distributed in public canteens of northeast italy. | over the past few years, the demand for the introduction of fish products in public canteens (schools, hospitals and nursing-homes) has grown due to their good nutritional proprieties. the particular health conditions and sensitivity of some groups of consumers exposes them to greater risks of food poisoning. it is therefore important to monitor the raw materials that end up in mass catering implementing strategies of mass catering control, both with self-monitoring strategies and with regular c ... | 2016 | 27995098 |
| the effect of preparation processes on the physicochemical characteristics and antibacterial activity of chitooligosaccharides. | the bioactivities of chitooligosaccharides are markedly influenced by the degree of acetylation, degree of polymerization or molecular weight and pattern of acetylation. thus, it is crucial to identify reproducible processes that will give rise to well-defined chitooligosaccharides and establish methods for their posterior physicochemical characterization in order to advance in the knowledge of their bioactivity. chitooligosaccharides were prepared by two different processes. the first used chit ... | 2017 | 27987925 |
| preparation and characterisation of chlorogenic acid-gelatin: a type of biologically active film for coating preservation. | chlorogenic acid (cga), a type of plant polyphenol, was conjugated onto gelatin (gel) to prepare a novel coating material for the preservation of fresh seafood. the optimal reaction molar ratio of cga to gelatin (4:1) was determined according to the cga content in the cga-gel conjugate. cga was confirmed to be successfully conjugated onto gelatin by (1)h nuclear magnetic resonance and fourier transform-infrared spectroscopy. the antioxidant activity of cga-gel was proven to be higher than that o ... | 2017 | 27979255 |
| microbial quality and suitability of roof-harvested rainwater in rural villages for crop irrigation and domestic use. | the study aimed at assessing the microbiological quality and suitability of roof-harvested rainwater (rhrw) for crop irrigation and domestic use. in total, 80 rainwater tanks (246 samples) across three rural villages (ga-molepane, jericho and luthngele) were visited. culture-based techniques were used to isolate bacterial microbes and identities were confirmed using matrix-assisted laser desorption/ionization time of flight (maldi-tof-ms). uncultured fungal populations were also identified using ... | 2016 | 27959874 |
| modeling stochastic variability in the numbers of surviving salmonella enterica, enterohemorrhagic escherichia coli, and listeria monocytogenes cells at the single-cell level in a desiccated environment. | despite effective inactivation procedures, small numbers of bacterial cells may still remain in food samples. the risk that bacteria will survive these procedures has not been estimated precisely because deterministic models cannot be used to describe the uncertain behavior of bacterial populations. we used the poisson distribution as a representative probability distribution to estimate the variability in bacterial numbers during the inactivation process. strains of four serotypes of salmonella ... | 2017 | 27940547 |
| microfluidic air sampler for highly efficient bacterial aerosol collection and identification. | the early warning capability of the presence of biological aerosol threats is an urgent demand in ensuing civilian and military safety. efficient and rapid air sample collection in relevant indoor or outdoor environment is a key step for subsequent analysis of airborne microorganisms. herein, we report a portable battery-powered sampler that is capable of highly efficient bioaerosol collection. the essential module of the sampler is a polydimethylsiloxane (pdms) microfluidic chip, which consiste ... | 2016 | 27934096 |
| removal of listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments. | the effects of pronase (prn), cellulase (cel) or dnasei alone or combined with benzalkonium chloride (bac) against listeria monocytogenes-carrying biofilms were assayed. the best removal activity against l. monocytogenes-escherichia coli biofilms was obtained using dnasei followed by prn and cel. subsequently, a modified logistic model was used to quantify the combined effects of prn or dnasei with bac. a better bac performance after prn compared to dnasei eradicating l. monocytogenes was observ ... | 2017 | 27918204 |
| microbiological quality and variability of natural microbiota in croatian cheese maturing in lambskin sacks. | as in the traditional production of cheese in lambskin sacks raw cow's or sheep's milk is mostly used, the purpose of this study is to see how the production affects the microbiological quality of the cheese. to do that, we tested 39 samples of raw cow's and sheep's milk, curd, ripened cheese (15, 30 and 45 days) and lambskin sacks for native microbial population. two-thirds of the milk, curd and cheese samples had higher counts of staphylococci and enterobacteria than permitted by regulations. ... | 2016 | 27904402 |
| improved properties and microbiological safety of novel cottage cheese containing spices. | the study focuses on developing novel cottage cheese containing spices with acceptable sensory properties, increased biological value and extended shelf life. thirty types of cheese with added fresh or dried parsley, dill, pepper, garlic and rosemary were produced. characterisation of phenolic compounds, antioxidant capacity and antibacterial activity of spices and cheese samples were evaluated. the cheese containing fresh pepper and fresh and dried herbs showed excellent sensory properties, wit ... | 2015 | 27904380 |
| antimicrobial activity of whey protein films supplemented with lactobacillus sakei cell-free supernatant on fresh beef. | the aim of this work was to evaluate the antimicrobial activity of whey protein isolate (wpi) films supplemented with lactobacillus sakei nrrl b-1917 cell-free supernatant on beef inoculated with escherichia coli atcc 25922 or listeria monocytogenes scott a; additionally, sensory evaluation was performed on wrapped beef cubes. supernatant concentrates were obtained from lb. sakei cultures in mrs broth after centrifugation, filtering, and freeze-drying. films were prepared with wpi (3% w/w), algi ... | 2017 | 27889150 |
| cationic nanostructures against foodborne pathogens. | | 2016 | 27881979 |
| antibacterial potential of jatropha curcas synthesized silver nanoparticles against food borne pathogens. | the aqueous leaf extract of jatropha curcas was used for the synthesis of silver nanoparticles (jc-agnps) which were further evaluated for its antibacterial potential against food borne pathogens. j. curcas leaf extract could synthesize stable silver nanoparticles (zeta potential: -23.4 mv) with absorption band at 430 nm. fourier transform infrared spectroscopy indicated various biological compounds responsible for capping and stabilizing jc-agnps in suspension, while the presence of silver was ... | 2016 | 27877160 |
| comparative genomics reveals the diversity of restriction-modification systems and dna methylation sites in listeria monocytogenes. | listeria monocytogenes is a bacterial pathogen that is found in a wide variety of anthropogenic and natural environments. genome sequencing technologies are rapidly becoming a powerful tool in facilitating our understanding of how genotype, classification phenotypes, and virulence phenotypes interact to predict the health risks of individual bacterial isolates. currently, 57 closed l. monocytogenes genomes are publicly available, representing three of the four phylogenetic lineages, and they sug ... | 2017 | 27836852 |
| in vitro evaluation of bacteriocin-like inhibitory substances produced by lactic acid bacteria isolated during traditional sicilian cheese making. | bacteriocins are antimicrobial proteins produced by bacteria that inhibit the growth of other bacteria with a bactericidal or bacteriostatic mode of action. many lactic acid bacteria (lab) produce a high diversity of different bacteriocins. bacteriocinogenic lab are generally recognised as safe (gras) and useful to control the frequent development of pathogens and spoilage microorganisms. for this reason they are commonly used as starter cultures in food fermentations. in this study, the authors ... | 2016 | 27800430 |
| enoyl-acyl carrier protein reductase i (fabi) is essential for the intracellular growth of listeria monocytogenes. | enoyl-acyl carrier protein reductase catalyzes the last step in each elongation cycle of type ii bacterial fatty acid synthesis and is a key regulatory protein in bacterial fatty acid synthesis. genes of the facultative intracellular pathogen listeria monocytogenes encode two functional enoyl-acyl carrier protein isoforms based on their ability to complement the temperature-sensitive growth phenotype of escherichia coli strain jp1111 [fabi(ts)]. the fabi isoform was inactivated by the fabi selec ... | 2016 | 27736774 |
| the scavenger receptor ssc5d physically interacts with bacteria through the srcr-containing n-terminal domain. | the scavenger receptor cysteine-rich (srcr) family comprises a group of membrane-attached or secreted proteins that contain one or more modules/domains structurally similar to the membrane distal domain of type i macrophage scavenger receptor. although no all-inclusive biological function has been ascribed to the srcr family, some of these receptors have been shown to recognize pathogen-associated molecular patterns (pamp) of bacteria, fungi, or other microbes. ssc5d is a recently described solu ... | 2016 | 27790215 |
| isolation and partial characterization of 3 nontoxic d-galactose-specific isolectins from seeds of momordica balsamina. | three isolectins denoted hereforth mbal-30, mbal-60, and mbal-80 were isolated from seeds extract of momordica balsamina by 30%, 60%, and 80% ammonium sulfate saturations, respectively. the native molecular weights of these lectins, as judged by gel filtration, were 108, 56, and 160 kda, respectively. on sds-page, under reduced condition, 27 kda band was obtained for all isolectins. the lectins hemagglutinating activities were variably inhibited by d-galactose (minimum inhibitory concentrations ... | 2017 | 27774692 |
| methodology for identification of pore forming antimicrobial peptides from soy protein subunits β-conglycinin and glycinin. | antimicrobial peptides (amps) inactivate microbial cells through pore formation in cell membrane. because of their different mode of action compared to antibiotics, amps can be effectively used to combat drug resistant bacteria in human health. amps can also be used to replace antibiotics in animal feed and immobilized on food packaging films. in this research, we developed a methodology based on mechanistic evaluation of peptide-lipid bilayer interaction to identify amps from soy protein. produ ... | 2016 | 27612614 |
| in vitro activity of plant extracts against biofilm-producing food-related bacteria. | the identification of effective antimicrobial agents also active on biofilms is a topic of crucial importance in food and industrial environment. for that purpose methanol extracts of turkish plants, ficus carica l., juglans regia l., olea europaea l., punica granatum l. and rhus coriaria l., were investigated. among the extracts, p. granatum l. and r. coriaria l. showed the best antibacterial activity with minimum inhibitory concentrations (mic) of 78-625μg/ml for listeria monocytogenes and sta ... | 2016 | 27591384 |
| a counterselection method for lactococcus lactis genome editing based on class iia bacteriocin sensitivity. | in this paper, we present a new counterselection method for deleting fragments from lactococcus lactis chromosome. the method uses a non-replicating plasmid vector, which integrates into the chromosome and makes the cell sensitive to bacteriocins. the integration vector carries puc ori functional in escherichia coli but not in l. lactis, an erythromycin resistance gene for selecting single crossover integrants, and two fragments from l. lactis chromosome for homologous recombinations. in additio ... | 2016 | 27654656 |
| reduced susceptibilities to biocides and resistance to antibiotics in food-associated bacteria following exposure to quaternary ammonium compounds. | our aim was to assess the effects of step-wise exposure to didecyl dimethyl ammonium chloride (ddac) on the antimicrobial (antibiotics and biocides) susceptibilities of food-associated bacterial strains. | 2016 | 27481186 |
| microfluidic cantilever detects bacteria and measures their susceptibility to antibiotics in small confined volumes. | in the fight against drug-resistant bacteria, accurate and high-throughput detection is essential. here, a bimaterial microcantilever with an embedded microfluidic channel with internal surfaces chemically or physically functionalized with receptors selectively captures the bacteria passing through the channel. bacterial adsorption inside the cantilever results in changes in the resonance frequency (mass) and cantilever deflection (adsorption stress). the excitation of trapped bacteria using inf ... | 2016 | 27698375 |
| in vivo and in vitro effects of selected antioxidants on rabbit meat microbiota. | the purpose of this study was to investigate the effect of dietary vitamin e or economase™ supplementation on the growth of several background/pathogenic bacteria on rabbit carcasses and hamburgers during refrigerated storage. for 51days, 270 new zealand rabbits received either a basal diet, or experimental diets enriched with 100 or 200mg/kg of vitamin e or economase™. the bacteria studied were salmonella, listeria monocytogenes, pseudomonas, enterobacteriaceae, escherichia coli, coagulase-posi ... | 2017 | 27664938 |
| identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected lactobacillus strains. | this study aimed to identify lactic acid bacteria (lab) in byproducts of fruit (malpighia glabra l., mangifera indica l., annona muricata l., and fragaria vesca l.) pulp processing. fifty strains of lab were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) and 16s rrna gene sequence (16s rrna) analysis. species belonging to lactobacillus genus were the predominant lab in all fruit pulp processing byproducts. the average congruency betwe ... | 2016 | 27625647 |
| comprehensive evaluation and implementation of improvement actions in butcher shops. | foodborne pathogens can cause acute and chronic diseases and produce a wide range of symptoms. since the consumption of ground beef is a risk factor for infections with some bacterial pathogens, we performed a comprehensive evaluation of butcher shops, implemented improvement actions for both butcher shops and consumers, and verified the impact of those actions implemented. a comprehensive evaluation was made and risk was quantified on a 1-100 scale as high-risk (1-40), moderate-risk (41-70) or ... | 2016 | 27618439 |
| presence of escherichia coli o157, salmonella spp., and listeria monocytogenes in raw ovine milk destined for cheese production and evaluation of the equivalence between the analytical methods applied. | italy is one of the main producers and exporters of cheese made from unpasteurized sheep milk. since raw milk and its derived products are known sources of human infections, cheese produced from raw sheep milk could pose a microbiological threat to public health. hence, the objectives of the study were: to characterize the potential risk of the presence of pathogens escherichia coli o157, listeria monocytogenes, and salmonella in raw ovine milk destined for cheese production obtained from all th ... | 2016 | 27607835 |
| development of a sandwich elisa for ehec o157:h7 intimin γ1. | enterohemorrhagic escherichia coli (ehec) o157:h7 is a zoonotic pathogen of worldwide importance that causes foodborne infections in humans. intimin gamma 1 (intimin γ1) is one of the most important outer membrane proteins required for ehec's intimate adhesion to epithelial cells. here, we generated a polyclonal antibody (pab) and a monoclonal antibody (mab) against intimin γ1 to develop a double antibody sandwich elisa (das-elisa) with increased sensitivity and specificity for measuring ehec o1 ... | 2016 | 27603508 |
| characterization of egg white antibacterial properties during the first half of incubation: a comparative study between embryonated and unfertilized eggs. | egg white is an important contributor to the protection of eggs against bacterial contaminations during the first half of incubation (day zero to 12), prior to the egg white transfer into the amniotic fluid to be orally absorbed by the embryo. this protective system relies on an arsenal of antimicrobial proteins and on intrinsic physicochemical properties that are generally unfavorable for bacterial multiplication and dissemination. some changes in these parameters can be observed in egg white d ... | 2016 | 27601682 |
| do bacterial cell numbers follow a theoretical poisson distribution? comparison of experimentally obtained numbers of single cells with random number generation via computer simulation. | we investigated a bacterial sample preparation procedure for single-cell studies. in the present study, we examined whether single bacterial cells obtained via 10-fold dilution followed a theoretical poisson distribution. four serotypes of salmonella enterica, three serotypes of enterohaemorrhagic escherichia coli and one serotype of listeria monocytogenes were used as sample bacteria. an inoculum of each serotype was prepared via a 10-fold dilution series to obtain bacterial cell counts with me ... | 2016 | 27554145 |
| modeling optimal process conditions for uv-heat inactivation of foodborne pathogens in liquid foods. | the combination of ultraviolet radiation and heat (uv-h treatment) has been demonstrated as a promising strategy to overcome the limited uv germicidal effect in fruit juices. nonetheless, there are so far no data regarding the efficacy of the combined process for the inactivation of bacterial foodborne pathogens in other liquid foods with different ph and composition. in this investigation, the optimum uv-h processing conditions for the inactivation of escherichia coli, salmonella typhimurium, l ... | 2016 | 27554141 |
| rapid colorimetric sensing platform for the detection of listeria monocytogenes foodborne pathogen. | listeria monocytogenes is a serious cause of human foodborne infections worldwide, which needs spending billions of dollars for inspection of bacterial contamination in food every year. therefore, there is an urgent need for rapid, in-field and cost effective detection techniques. in this study, rapid, low-cost and simple colorimetric assay was developed using magnetic nanoparticles for the detection of listeria bacteria. the protease from the listeria bacteria was detected using d-amino acid su ... | 2016 | 27543841 |
| chitosan grafted monomethyl fumaric acid as a potential food preservative. | the present study aims at in vitro antibacterial and antioxidant activity evaluation of chitosan modified with monomethyl fumaric acid (mfa) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (edc) as mediator. three different kinds of chitosan derivatives ch-ds-1,ch-ds-2 and ch-ds-3 were synthesized by feeding different concentration of mfa. the chemical structures of resulting materials were characterized by (1)h nmr, (13)c nmr, hr-xrd, ft-ir and tnbs assay. the results showed that ch-ds-1, ... | 2016 | 27516253 |
| preparation of pectin/silver nanoparticles composite films with uv-light barrier and properties. | silver nanoparticles (agnps) was synthesized by a green method using an aqueous extract of caesalpinia mimosoides lamk (cmle) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films. the agnps were spherical in shape with the size in the range of 20-80nm and showed the absorption peak around 500nm. the pectin/agnps composite film exhibited characteristic absorption peak of agnps at 480nm. the surface color and light transmittance o ... | 2016 | 27492557 |
| in vitro safety assessments and antimicrobial activities of lactobacillus rhamnosus strains isolated from a fermented mare's milk. | safety and probiotic characteristics such as antimicrobial activities of three lactobacillus rhamnosus strains, fsmm15, fsmm22 and fsmm26, previously isolated as potential probiotics from fermented mare's milk were investigated. the three fsmm strains were susceptible to ampicillin, gentamycin, kanamycin, streptomycin, tetracycline and chloramphenicol, whereas they were resistant to erythromycin (minimal inhibitory concentration (mic) = 4-8 µg/ml) and clindamycin (mic = 4 µg/ml); bioconversion o ... | 2017 | 27476815 |
| identification of the agr peptide of listeria monocytogenes. | listeria monocytogenes (lm) is an important food-borne human pathogen that is able to strive under a wide range of environmental conditions. its accessory gene regulator (agr) system was shown to impact on biofilm formation and virulence and has been proposed as one of the regulatory mechanisms involved in adaptation to these changing environments. the lm agr operon is homologous to the staphylococcus aureus system, which includes an agrd-encoded autoinducing peptide that stimulates expression o ... | 2016 | 27446029 |
| potential of the polymer poly-[2-(tert-butylamino) methylstyrene] as antimicrobial packaging material for meat products. | the objective of the study was to investigate the antimicrobial potential of a new sam(®) polymer poly(tbams) as packaging material for meat products. | 2016 | 27427869 |
| duplex identification of staphylococcus aureus by aptamer and gold nanoparticles. | staphylococcus aureus is the top common pathogen causing infections and food poisoning. identification of s. aureus is crucial for the disease diagnosis and regulation of food hygiene. herein, we report an aptamer-aunps based method for duplex identification of s. aureus. using aunps as an indicator, sa23, an aptamer against s. aureus, can well identify its target from escherichia coli, listeria monocytogenes and pseudomonas aeruginosa. furthermore, we find citrate-coated aunps can strongly bind ... | 2016 | 27427591 |
| bacterial meningitis in solid organ transplant recipients: a population-based prospective study. | solid organ transplant (sot) recipients are at risk of infections of the central nervous system. however, the incidence and clinical course of bacterial meningitis in sot recipients are unclear. we studied occurrence, disease course, and prognosis of bacterial meningitis in sot recipients in the netherlands. | 2016 | 27385317 |
| in vitro and in vivo probiotic assessment of leuconostoc mesenteroides p45 isolated from pulque, a mexican traditional alcoholic beverage. | pulque is a mexican traditional alcoholic, non-distilled, fermented beverage produced by the fermentation of the sap, known as aguamiel, extracted from several maguey (agave) species. pulque has traditionally been considered a healthy beverage due to its nutrient content and also a traditional medicine for the treatment of gastrointestinal disorders and intestinal infections. during pulque fermentation, the development of acidity, alcohol and viscosity define its final sensorial properties, deve ... | 2016 | 27375977 |
| survival of foodborne pathogens in natural cracked olive brines. | this work reports the survival (challenge tests) of foodborne pathogen species (escherichia coli, staphylococcus aureus, listeria monocytogenes, and salmonella enterica) in aloreña de málaga table olive brines. the inhibitions were fit using a log-linear model with tail implemented in ginafit excel software. the olive brine had a considerable inhibitory effect on the pathogens. the residual (final) populations (fp) after 24 h was below detection limit (<1.30 log10 cfu/ml) for all species assayed ... | 2016 | 27375250 |
| bioactive packaging using antioxidant extracts for the prevention of microbial food-spoilage. | bioactive food packaging is an innovative approach for the prevention of the growth of food-spoilage microorganisms. four active extracts from agroindustrial subproducts (eucalyptus wood, almond shells, corn cobs and grape pomace) with demonstrated antioxidant activity have been investigated for bestowing antimicrobial activity to bioactive packaging. to carry out this evaluation, the antioxidant extracts were tested against five food pathogenic bacteria, namely, escherichia coli, pseudomonas ae ... | 2016 | 27363903 |
| changes in aerobic plate and escherichia coli-coliform counts and in populations of inoculated foodborne pathogens on inshell walnuts during storage. | after harvest, inshell walnuts are dried using low-temperature forced air and are then stored in bins or silos for up to 1 year. to better understand the survival of bacteria on inshell walnuts, aerobic plate counts (apcs) and escherichia coli?coliform counts (eccs) were evaluated during commercial storage (10 to 12°c and 63 to 65% relative humidity) over 9 months. apcs decreased by 1.4 to 2.0 log cfu per nut during the first 5 months of storage, and eccs decreased by 1.3 to 2.2 log cfu per nut ... | 2016 | 27357033 |
| multi-criteria framework as an innovative tradeoff approach to determine the shelf-life of high pressure-treated poultry. | a multi-criteria framework combining safety, hygiene and sensorial quality was developed to investigate the possibility of extending the shelf-life and/or removing lactate by applying high hydrostatic pressure (hhp) in a ready-to-cook (rtc) poultry product. for this purpose, salmonella and listeria monocytogenes were considered as safety indicators and escherichia coli as hygienic indicator. predictive modeling was used to determine the influence of hhp and lactate concentration on microbial gro ... | 2016 | 27343425 |
| foodborne bacterial pathogens in retail prepacked ready-to-eat mixed ingredient salads. | prepacked ready-to-eat mixed ingredient salads (rte salads) are readily available whole meals that include a variety of ingredients such as raw vegetables, cooked meat, and pasta. as part of a trend toward healthy convenience foods, rte salads have become an increasingly popular product among consumers. however, data on the incidence of foodborne pathogens in rte salads are scarce. in this study, the microbiological safety of 141 rte salads containing chicken, ham, or smoked salmon was investiga ... | 2016 | 27296602 |
| estimating bacterial pathogen levels in new zealand bulk tank milk. | zoonotic bacteria such as campylobacter, listeria, and shiga toxin-producing escherichia coli have been found in bulk tank milk in many countries, and the consumption of raw milk has been implicated in outbreaks of disease in new zealand. fecal contamination at milking is probably the most common source of pathogenic bacteria in bulk tank milk. raw milk was collected from 80 new zealand dairy farms during 2011 and 2012 and tested periodically for campylobacter, e. coli o157, listeria monocytogen ... | 2016 | 27296424 |
| coliform detection in cheese is associated with specific cheese characteristics, but no association was found with pathogen detection. | coliform detection in finished products, including cheese, has traditionally been used to indicate whether a given product has been manufactured under unsanitary conditions. as our understanding of the diversity of coliforms has improved, it is necessary to assess whether coliforms are a good indicator organism and whether coliform detection in cheese is associated with the presence of pathogens. the objective of this study was (1) to evaluate cheese available on the market for presence of colif ... | 2016 | 27289158 |
| antioxidant, antibacterial, and antiproliferative activities of free and bound phenolics from peel and flesh of fuji apple. | this study was conducted to investigate the antioxidant, antibacterial, and antiproliferative activities of flesh free (ff), flesh bound (fb), peel free (pf), and peel bound (pb) phenolics from fuji apple. the pb, which had highest total phenolic contents (126.15 ± 2.41 mg/100 g wet weight) and lowest total carbohydrate contents (34.68 ± 2.78 mg/100 g wet weight), showed the strongest 2,2'-azinobis-(3-ethylbenthiazoline-6-sulphonate) (abts) radical scavenging activity (ec50 = 0.36 ± 0.02 mg/ml), ... | 2016 | 27272442 |
| bacterial quality and safety of packaged fresh leafy vegetables at the retail level in finland. | consumption of packaged fresh leafy vegetables, which are convenient ready-to-eat products, has increased during the last decade. the number of foodborne outbreaks associated with these products has concurrently increased. in our study, (1) label information, (2) o2/co2 composition, (3) bacterial quality and (4) safety of 100 fresh leafy vegetables at the retail level were studied in finland during 2013. bacterial quality was studied using aerobic bacteria (ab) and coliform bacteria (cb) counts, ... | 2016 | 27257744 |
| effects of chitin nano-whiskers on the antibacterial and physicochemical properties of maize starch films. | we investigated the effects of chitin nano-whiskers (cnws) on the antibacterial and physiochemical properties of maize starch-based films. the microstructures, crystalline structures, and thermal, mechanical and barrier properties of the nanocomposite films were characterized by using transmission electron microscopy, x-ray diffraction analysis, thermogravimetric, differential scanning calorimeter, and texture profile analysis. the tensile strength of the maize starch films increased from 1.64mp ... | 2016 | 27178943 |
| characterization of two listeria innocua chitinases of different sizes that were expressed in escherichia coli. | two putative chitinase genes, lin0153 and lin1996, from the nonpathogenic bacterium listeria innocua were expressed in escherichia coli, and the gene products were characterized. the genes were close homologs of chitinases from the pathogenic bacterium listeria monocytogenes, in which chitinases and chitin-binding proteins play important roles in pathogenesis in mice-infection models. the purified recombinant enzymes that are different in size, linchi78 (lin0153 product) and linchi35 (lin1996 pr ... | 2016 | 27138200 |
| cellulose aerogels functionalized with polypyrrole and silver nanoparticles: in-situ synthesis, characterization and antibacterial activity. | green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials. in this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive naoh/polyethylene glycol solution was used as cellulose solvent. prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ ... | 2016 | 27112885 |
| preparations and characterization of alginate/silver composite films: effect of types of silver particles. | alginate-based films reinforced with different types of silver particles such as metallic silver (agm), silver zeolite (agz), citrate reduced silver nanoparticles (agnp(c)), laser ablated silver nanoparticles (agnp(la)), and silver nitrate (agno3) were prepared using a solvent casting method and the effect of silver particles on the optical, mechanical, water vapor barrier, and antimicrobial properties the composite films was evaluated. size and shape of the silver particles were varied dependin ... | 2016 | 27112867 |
| rifampicin- and rifabutin-resistant listeria monocytogenes strains isolated from food products carry mutations in rpob gene. | the aim of this study was to investigate the mechanism of rifampicin resistance in listeria monocytogenes strains isolated from different types of food and the impact of specific mutations in the rpob gene on susceptibility to different antimicrobial agents and on fitness cost. fifteen spontaneous rifampicin-resistant strains were selected. the dna regions corresponding to clusters i-ii, iii, and n-terminal end of the rpob gene of escherichia coli were amplified and sequenced, leading to the ide ... | 2016 | 27105395 |
| microbiological quality of the water used in agriculture in sardinia. | the microbiological quality of the water used in irrigation is crucial for the safety of products, such as fruit and vegetables, especially when destined to be consumed raw. however, the microbiological quality of this water is not defined at a community regulatory level or at a national level. | 2016 | 27071326 |