| bacteriocin activity against various pathogens produced by pediococcus pentosaceus vj13 isolated from idly batter. | bacteriocins, an antimicrobial peptide, is known to have wide spectrum antimicrobial activity against various pathogens. because they are easily digested in the intestine, they are considered as safe and are widely used as food preservatives. hence their purification and characterization have attracted considerable attraction, especially for those having activity against human pathogens. in this study, the bacteriocin produced by pediococcus pentosaceus vj13 was precipitated with cold acetone an ... | 2013 | 23754544 |
| puerperal sepsis in a rural hospital in sudan. | increasingly, women in rural areas in sudan reported to hospital with puerperal infections. | 2013 | 23678336 |
| targeting agr- and agr-like quorum sensing systems for development of common therapeutics to treat multiple gram-positive bacterial infections. | invasive infection by the gram-positive pathogen staphylococcus aureus is controlled by a four gene operon, agr that encodes a quorum sensing system for the regulation of virulence. while agr has been well studied in s. aureus, the contribution of agr homologues and analogues in other gram-positive pathogens is just beginning to be understood. intriguingly, other significant human pathogens, including clostridium perfringens, listeria monocytogenes, and enterococcus faecalis contain agr or analo ... | 2013 | 23598501 |
| bacteriocin production by bifidobacterium spp. a review. | bacteriocins are ribosomally-synthesized antibacterial peptides. these compounds are produced by a broad variety of different bacteria belonging mainly to the genus bifidobacterium, to which health promoting properties have frequently been attributed. however, despite the fact that the identification of bifidobacterium-associated bacteriocins was first reported in 1980 and that they exhibit antimicrobial activity against pathogenic microorganisms such as listeria monocytogenes, clostridium perfr ... | 2013 | 23384878 |
| nonclinical development of bcg replacement vaccine candidates. | the failure of current mycobacterium bovis bacille calmette-guérin (bcg) vaccines, given to neonates to protect against adult tuberculosis and the risk of using these live vaccines in hiv-infected infants, has emphasized the need for generating new, more efficacious and safer replacement vaccines. with the availability of genetic techniques for constructing recombinant bcg (rbcg) strains containing well-defined gene deletions or insertions, new vaccine candidates are under evaluation at both the ... | 2013 | 26343962 |
| ranking the disease burden of 14 pathogens in food sources in the united states using attribution data from outbreak investigations and expert elicitation. | understanding the relative public health impact of major microbiological hazards across the food supply is critical for a risk-based national food safety system. this study was conducted to estimate the u.s. health burden of 14 major pathogens in 12 broad categories of food and to then rank the resulting 168 pathogen-food combinations. these pathogens examined were campylobacter, clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, norovirus, salmonella enterica, toxoplasma ... | 2012 | 22980012 |
| survey of naturally and conventionally cured commercial frankfurters, ham, and bacon for physio-chemical characteristics that affect bacterial growth. | natural and organic food regulations preclude the use of sodium nitrite/nitrate and other antimicrobials for processed meat products. consequently, processors have begun to use natural nitrate/nitrite sources, such as celery juice/powder, sea salt, and turbinado sugar, to manufacture natural and organic products with cured meat characteristics but without sodium nitrite. the objective of this study was to compare physio-chemical characteristics that affect clostridium perfringens and listeria mo ... | 2012 | 22857852 |
| immunohistochemical search for viral and bacterial antigens in crohn's disease. | recent studies show that diseased intestinal tissues of patients with crohn's disease (cd) contain obstructed lymphatics, granulomas, and tertiary lymphoid organs, representing responses to persistent antigen. | 2013 | 22537638 |
| effect of temperature on microbial growth rate-mathematical analysis: the arrhenius and eyring-polanyi connections. | the objective of this work is to develop a mathematical model for evaluating the effect of temperature on the rate of microbial growth. the new mathematical model is derived by combination and modification of the arrhenius equation and the eyring-polanyi transition theory. the new model, suitable for both suboptimal and the entire growth temperature ranges, was validated using a collection of 23 selected temperature-growth rate curves belonging to 5 groups of microorganisms, including pseudomona ... | 2011 | 22417589 |
| copsin, a novel peptide-based fungal antibiotic interfering with the peptidoglycan synthesis. | fungi and bacteria compete with an arsenal of secreted molecules for their ecological niche. this repertoire represents a rich and inexhaustible source for antibiotics and fungicides. antimicrobial peptides are an emerging class of fungal defense molecules that are promising candidates for pharmaceutical applications. based on a co-cultivation system, we studied the interaction of the coprophilous basidiomycete coprinopsis cinerea with different bacterial species and identified a novel defensin, ... | 2014 | 25342741 |
| identification of coxiella burnetii cd8+ t-cell epitopes and delivery by attenuated listeria monocytogenes as a vaccine vector in a c57bl/6 mouse model. | coxiella burnetii is a gram-negative bacterium that causes acute and chronic q fever. because of the severe adverse effect of whole-cell vaccination, identification of immunodominant antigens of c. burnetii has become a major focus of q fever vaccine development. we hypothesized that secreted c. burnetii type iv secretion system (t4ss) effectors may represent a major class of cd8(+) t-cell antigens, owing to their cytosolic localization. twenty-nine peptides were identified that elicited robust ... | 2016 | 27703037 |
| comparison of desiccation tolerance among listeria monocytogenes, escherichia coli o157:h7, salmonella enterica, and cronobacter sakazakii in powdered infant formula. | bacterial pathogens such as listeria monocytogenes, escherichia coli o157:h7, salmonella enterica, and cronobacter sakazakii have demonstrated long-term survival in/on dry or low-water activity (aw) foods. however, there have been few comparative studies on the desiccation tolerance among these bacterial pathogens separately in a same food matrix. in the present study, the survival kinetics of the four bacterial pathogens separately inoculated onto powdered infant formula as a model low-aw food ... | 2015 | 25581184 |
| [magnetic beads adsorption-pcr detection method of four common foodborne pathogens in food]. | to evaluate the adsorption capacity of magnetic beads for common foodborne pathogens. | 2014 | 25199281 |
| bacterial maximum non-inhibitory and minimum inhibitory concentrations of different water activity depressing solutes. | the nacl mnics (maximum non-inhibitory concentrations) and mics (minimum inhibitory concentrations) for growth of various strains of six bacterial species were determined and then compared with those obtained for seven other solutes. the influence of prior growth conditions on the mnics and mics was also evaluated. no significant changes on the mnics and mics were found among the strains studied within each species. among all factors investigated, only growth phase -for gram-negatives- and growt ... | 2014 | 25090605 |
| evaluation of the microbial safety of child food of animal origin in greece. | foodborne illness is a major cause of morbidity and mortality especially for children, even in the developed world. the aim of this study was to assess the microbial safety of food of animal origin intended for consumption by children in greece. sampling involved 8 categories of retail products and was completed with a collection of 850 samples. these were tested by pcr and/or culture for listeria monocytogenes, campylobacter spp., escherichia coli o157, salmonella spp., cronobacter sakazakii, b ... | 2014 | 24506312 |
| structure-activity relationship of synthetic variants of the milk-derived antimicrobial peptide αs2-casein f(183-207). | template-based studies on antimicrobial peptide (amp) derivatives obtained through manipulation of the amino acid sequence are helpful to identify properties or residues that are important for biological activity. the present study sheds light on the importance of specific amino acids of the milk-derived αs2-casein f(183-207) peptide to its antibacterial activity against the food-borne pathogens listeria monocytogenes and cronobacter sakazakii. trimming of the peptide revealed that residues at t ... | 2013 | 23793637 |
| the pathogen-annotated tracking resource network (patrn) system: a web-based resource to aid food safety, regulatory science, and investigations of foodborne pathogens and disease. | investigation of foodborne diseases requires the capture and analysis of time-sensitive information on microbial pathogens that is derived from multiple analytical methods and sources. the web-based pathogen-annotated tracking resource network (patrn) system (www.patrn.net) was developed to address the data aggregation, analysis, and communication needs important to the global food safety community for the investigation of foodborne disease. patrn incorporates a standard vocabulary for describin ... | 2013 | 23541197 |
| safety assessment and probiotic evaluation of enterococcus faecium yf5 isolated from sourdough. | enterococcus faecium yf5, a strain previously isolated from sourdough, was assessed for safety and probiotic potential. its virulence and antibiotic resistant phenotypes (cytolysin and gelatinase production, antibiotic susceptibility) and genes (cyla, gele, ace, agg, esp, and vana) were surveyed. results indicated that the tested virulence determinants were nontoxic. in addition, e. faecium yf5 was sensitive to 3 antibiotics such as amoxicillin, vancomycin, and chloramphenicol. furthermore, resu ... | 2013 | 23488799 |
| microbiology of processed edible insect products - results of a preliminary survey. | little is known of the microbiology of processed insect products. the present survey analysed a total of n=38 samples of deep-fried and spiced (acheta domesticus, locusta migratoria, and omphisa fuscidentalis), cooked in soy sauce ("tsukudani"; oxya yezoensis, vespula flaviceps, and bombyx mori), dried (a. domesticus, l. migatoria, alphitobius diaperinus, tenebrio molitor, b. mori, hermetia illucens, and musca domestica), powdered (h. illucens, t. molitor) and other (incl. deep-frozen b. mori an ... | 2017 | 27903420 |
| multicenter evaluation of biofire filmarray meningitis/encephalitis panel for detection of bacteria, viruses, and yeast in cerebrospinal fluid specimens. | rapid diagnosis and treatment of infectious meningitis and encephalitis are critical to minimize morbidity and mortality. comprehensive testing of cerebrospinal fluid (csf) often includes gram stain, culture, antigen detection, and molecular methods, paired with chemical and cellular analyses. these methods may lack sensitivity or specificity, can take several days, and require significant volume for complete analysis. the filmarray meningitis/encephalitis (me) panel is a multiplexed in vitro di ... | 2016 | 27335149 |
| a multi-target real-time pcr assay for rapid identification of meningitis-associated microorganisms. | a central nervous system (cns) infection, such as meningitis, is a serious and life-threatening condition. bacterial meningitis can be severe and may result in brain damage, disability or even death. rapid diagnosis of cns infections and identification of the pathogenic microorganisms are needed to improve the patient outcome. bacterial culture of a patient's cerebrospinal fluid (csf) is currently considered the "gold standard" for diagnosing bacterial meningitis. from the csf cultures researche ... | 2013 | 22450734 |
| development and application of loop-mediated isothermal amplification assays based on its-1 for rapid detection of toxoplasma gondii in pork. | the loop-mediated isothermal amplification (lamp) assay is a novel method that rapidly amplifies dna with high specificity and sensitivity under isothermal conditions. in this study, we established a lamp assay with six primers targeting a highly conserved region of toxoplasma gondii its-1 sequence. the amplification protocol completes within 30 min under isothermal condition in a 65°c water bath while specificity tests confirmed no cross-reactivity with dna templates of neospora caninum, eimeri ... | 2015 | 25624074 |
| lowest numbers of primary cd8(+) t cells can reconstitute protective immunity upon adoptive immunotherapy. | patients undergoing allogeneic hematopoietic stem cell transplantation (allo-hsct) are threatened by potentially lethal viral manifestations like cytomegalovirus (cmv) reactivation. because the success of today's virostatic treatment is limited by side effects and resistance development, adoptive transfer of virus-specific memory t cells derived from the stem cell donor has been proposed as an alternative therapeutic strategy. in this context, dose minimization of adoptively transferred t cells ... | 2014 | 24855206 |
| green conversion of agroindustrial wastes into chitin and chitosan by rhizopus arrhizus and cunninghamella elegans strains. | this article sets out a method for producing chitin and chitosan by cunninghamella elegans and rhizopus arrhizus strains using a green metabolic conversion of agroindustrial wastes (corn steep liquor and molasses). the physicochemical characteristics of the biopolymers and antimicrobial activity are described. chitin and chitosan were extracted by alkali-acid treatment, and characterized by infrared spectroscopy, viscosity and x-ray diffraction. the effectiveness of chitosan from c. elegans and ... | 2014 | 24853288 |
| characteristics of microbial biofilm on wooden vats ('gerles') in pdo salers cheese. | the purpose of this study was to characterize microbial biofilms from 'gerles' (wooden vats for making pdo salers cheese) and identify their role in milk inoculation and in preventing pathogen development. gerles from ten farms producing pdo salers cheese were subjected to microbial analysis during at least 4 periods spread over two years. they were distinguished by their levels of lactobacillus (between 4.50 and 6.01 log cfu/cm(2)), gram negative bacteria (between 1.45 and 4.56 log cfu/cm(2)), ... | 2012 | 22483401 |
| evaluation of the antioxidative, antibacterial, and anti-inflammatory effects of the aloe fermentation supernatant containing lactobacillus plantarum hm218749.1. | little work is done to develop aloe vera (av) using probiotics. to explore the potential benefits, the antioxidant effects and the antibacterial effects on foodborne pathogens of aloe fermentation supernatant were evaluated in vitro. our results indicated that the aloe fermentation supernatant fermented by lactobacillus plantarum hm218749.1 had very strong scavenging capacities of the dpph (86%), o2 (•-) (85%), (•)oh (76%), and fe(2+) chelation (82%) and reducing powers (242.5 mg/l), and the inh ... | 2016 | 27493450 |
| yeasts and hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil. | the aim of this work was to study the yeast populations and the main hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil. forty-two water buffalo mozzarella samples were purchased from retail outlets in belo horizonte. in addition, five samples of consecutive starter cultures, curd before acidification, acidified curd and mozzarella were collected at an industry in the city of oliveira. only three of the five water samples analyz ... | 2013 | 24516436 |
| effects of listeria monocytogenes egd-e and salmonella enterica ser. typhimurium lt2 chitinases on intracellular survival in dictyostelium discoideum and mammalian cell lines. | some bacterial pathogens produce chitinases as virulence factors during host infection. the molecular target of such enzymes in non-chitinous hosts remains uncertain. we studied the importance of listeria monocytogenes egd-e and salmonella enterica ser. typhimurium lt2 chitinases for intracellular survival in dictyostelium discoideum, and for salmonella, also infection of mammalian cell lines, and a mouse model. the salmonella chitinase did not contribute significantly to infection of d. discoid ... | 2015 | 25908871 |
| a galectin related protein from oplegnathus fasciatus: genomic, molecular, transcriptional features and biological responses against microbial pathogens. | galectins, a family of β-galactoside-binding lectins, are pattern recognition receptors that recognize pathogen-associated molecular patterns and are subsequently involved in the opsonization, phagocytosis, complement activation, and killing of microbes. here, we report a novel galectin related protein (grp) identified from rock bream (oplegnathus fasciatus), designated ofgal like b. the cdna of ofgal like b is 517 bp with an open reading frame (orf) of 438 bp, encoding 145 amino acids, with a s ... | 2016 | 26615008 |
| internalisation potential of escherichia coli o157:h7, listeria monocytogenes, salmonella enterica subsp. enterica serovar typhimurium and staphylococcus aureus in lettuce seedlings and mature plants. | the internalisation potential of listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7 and salmonella enterica subsp. enterica serovar typhimurium in lettuce was evaluated using seedlings grown in vermiculite in seedling trays as well as hydroponically grown lettuce. sterile distilled water was spiked with one of the four human pathogenic bacteria (10(5) cfu/ml) and used to irrigate the plants. the potential for pathogen internalisation was investigated over time using light mi ... | 2013 | 23708570 |
| assessment of safe enterococci as bioprotective cultures in low-acid fermented sausages combined with high hydrostatic pressure. | three bacteriocinogenic, non-aminogenic and non-virulent enterococcus strains (enterococcus faecium ctc8005, enterococcus devriesei ctc8006 and enterococcus casseliflavus ctc8003) were used as starter cultures in low-acid fermented sausages to assess their competitiveness and their bioprotective potential against listeria monocytogenes and staphylococcus aureus. the inoculated strains were successfully monitored by rapd-pcr. all the strains were able to grow, survive and dominate the endogenous ... | 2013 | 23200647 |
| study of vancomycin resistance in faecal enterococci from healthy humans and dogs in spain a decade after the avoparcin ban in europe. | one hundred and 26 faecal samples from healthy dogs (2009) and 157 faecal samples from healthy humans (2007) from la rioja region (spain) were tested to know the carriage of vancomycin-resistant enterococci (vre). vre with intrinsic resistance (vanc) were found in 12% of healthy dogs and humans (29 enterococcus gallinarum and four enterococcus casseliflavus). nevertheless, vre with acquired mechanism of resistance were not detected among these samples. four enterococcus faecalis isolates with va ... | 2013 | 22646722 |
| alanine-scanning mutational analysis of durancin gl reveals residues important for its antimicrobial activity. | durancin gl is a novel class iia bacteriocin with 43 residues produced by enterococcus durans 41d. this bacteriocin demonstrates narrow inhibition spectrum and potent antimicrobial activity against several listeria monocytogenes strains, including nisin-resistant l. monocytogenes nr30. a systematic alanine-scanning mutational analysis with site-directed mutagenesis was performed to analyze durancin gl residues important for antimicrobial activity and specificity. results showed that three mutati ... | 2015 | 26168032 |
| genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk. | lactic acid bacteria (lab, n = 57) were previously obtained from raw goat milk, identified as lactococcus spp. (n = 24) and enterococcus spp. (n = 33), and characterized as bacteriocinogenic. fingerprinting by pulsed field gel electrophoresis (pfge) demonstrated high genetic diversity, and 30 strains were selected and exhibited strong antimicrobial activity against 46 target strains (lab, spoilage, and foodborne pathogens). six strains (lactococcus lactis: glc03 and glc05; and enterococcus duran ... | 2015 | 25637509 |
| high inhibition of paenibacillus larvae and listeria monocytogenes by enterococcus isolated from different sources in tunisia and identification of their bacteriocin genes. | a total of 300 isolates of enterococcus, from different sources including faeces of poultry, cow and sheep, raw milk, ricotta cheese and water, in tunisia, were screened for their antibacterial activity. amongst them, 59 bacteriocin-producing strains were detected and identified by molecular methods. genes encoding for enta, entp, entb, entl50a/b, as-48 and bac31 bacteriocins were targeted by pcr. the bacteriocin-producing strains were assigned to the species enterococcus faecium, enterococcus f ... | 2014 | 24698417 |
| reduction by competitive bacteria of listeria monocytogenes in biofilms and listeria bacteria in floor drains in a ready-to-eat poultry processing plant. | the ability of listeria monocytogenes and two competitive exclusion (ce) bacteria, lactococcus lactis subsp. lactis strain c-1-92 and enterococcus durans strain 152, to form biofilms on coupons composed of different materials (stainless steel, plastic, rubber, glass, and silicone) was determined at 4 and 8 °c. biofilm characteristics were determined by scanning electron microscopy. l. monocytogenes produced well-formed biofilms within 24 h at 37 °c on coupon surfaces. treating listeria-laden bio ... | 2013 | 23575121 |
| antagonistic activity of lactobacillus reuteri strains on the adhesion characteristics of selected pathogens. | adhesion ability of probiotics is the key factor that decides their colonization in the gastrointestinal tract and potential to inhibit pathogens. therefore, adhesion ability can be considered as a key determinant for probiotic efficacy. presents study documents the antagonistic activity of viable/untreated, lithium chloride (licl) treated or heat-killed forms of eight probiotic lactobacillus reuteri strains on the adhesion characteristics of selected pathogens. all strains investigated were abl ... | 2017 | 28377765 |
| chemical content, antibacterial and antioxidant properties of essential oil extract from tunisian origanum majorana l. cultivated under saline condition. | essential oils of marjoram were extracted from plants, growing under non-saline and saline condition (75mm nacl). their antioxidant and antibaterial activity against six bacteria (enterococcus faecalis, escherichia coli, salmonella enteritidis, listeria ivanovii, listeria inocula, and listeria monocytogenes) were assessed. result showed that, (i) independently of salt treatment, marjoram essential oils inhibited the growth of most of the bacteria but in degrees. the least susceptible one was ent ... | 2016 | 28375110 |
| primary pyogenic ventriculitis caused by neisseria meningitidis: case report and review of the literature. | background. pyogenic ventriculitis is a well-known complication of meningitis, brain abscesses and intraventricular drains. primary pyogenic ventriculitis is a rare entity and few cases have been described so far. we report the first case of primary pyogenic ventriculitis in an adult caused by neisseria meningitidis and present an overview of all reported adult primary pyogenic ventriculitis cases in the english literature. methods. a pubmed search was performed using the terms ependymitis, vent ... | 2017 | 28348798 |
| beneficial and safety properties of a corynebacterium vitaeruminis strain isolated from the cow rumen. | corynebacterium vitaeruminis mru4 was isolated from the cow rumen and was differentiated from other isolates by rep-pcr and rapd and identified by 16s rrna sequencing. this strain presented higher survival rates for low ph and bile salts treatments, and it was able to survive and multiply in simulated gastric and intestinal environments. c. vitaeruminis mru4 had a 53.2% auto-aggregation rate, 42.4% co-aggregation rate with listeria monocytogenes scott a, 41.6% co-aggregation rate with enterococc ... | 2017 | 28258546 |
| diffusible substances from lactic acid bacterial cultures exert strong inhibitory effects on listeria monocytogenes and salmonella enterica serovar enteritidis in a co-culture model. | food-borne infections cause huge economic and human life losses. listeria monocytogenes and salmonella enterica serovar enteritidis are among the top ranking pathogens causing such losses. control of such infections is hampered by persistent contamination of foods and food-processing environments, resistance of pathogens to sanitizing agents, existence of heterogeneous populations of pathogens (including culturable and viable but non-culturable cells) within the same food items, and inability to ... | 2017 | 28202007 |
| synthesis, surfactant properties and antimicrobial activities of methyl glycopyranoside ethers. | a series of amphiphilic methyl glucopyranoside ethers incorporating various alkyl chain lengths has been synthesized from commercially available methyl glucopyranosides following an acetalisation/hydrogenolysis sequence. the amphiphilic properties of ethers and acetal intermediates were evaluated. both families exhibit excellent surfactant properties with a maximum efficiency obtained for compounds bearing a linear dodecyl chain (cmc = 0.012 mm, γsat. = 30 mn m(-1)). antimicrobial activity studi ... | 2017 | 28157594 |
| experience with rapid microarray-based diagnostic technology and antimicrobial stewardship for patients with gram-positive bacteremia. | objective to describe the impact of rapid diagnostic microarray technology and antimicrobial stewardship for patients with gram-positive blood cultures. design retrospective pre-intervention/post-intervention study. setting a 1,200-bed academic medical center. patients inpatients with blood cultures positive for staphylococcus aureus, enterococcus faecalis, e. faecium, streptococcus pneumoniae, s. pyogenes, s. agalactiae, s. anginosus, streptococcus spp., and listeria monocytogenes during the 6 ... | 2016 | 27767002 |
| development of pla films containing oregano essential oil (origanum vulgare l. virens) intended for use in food packaging. | consumers' concerns about the environment and health have led to the development of new food packaging materials avoiding petroleum-based matrices and synthetic additives. the present study has developed polylactic acid (pla) films containing different concentrations of essential oil from origanum vulgare l. virens (oeo). the effectiveness of this new active packaging was checked for use in ready-to-eat salads. a plasticising effect was observed when oeo was incorporated in pla films. the rest o ... | 2016 | 27455176 |
| antimicrobial and physicochemical characterization of biodegradable, nitric oxide-releasing nanocellulose-chitosan packaging membranes. | biodegradable composite membranes with antimicrobial properties consisting of nanocellulose fibrils (cnfs), chitosan, and s-nitroso-n-acetyl-d-penicillamine (snap) were developed and tested for food packaging applications. as a nitric oxide donor, snap was encapsulated into completely dispersed chitosan in 100 ml of 0.1 n acetic acid and was thoroughly mixed with cnfs to produce a composite membrane. the fabricated membranes had a uniform dispersion of chitosan and snap within the cnfs, which wa ... | 2016 | 27258235 |
| probiotic properties of lactic acid bacteria isolated from human milk. | the objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties. | 2016 | 27159339 |
| spatial organization of cell wall-anchored proteins at the surface of gram-positive bacteria. | bacterial surface proteins constitute an amazing repertoire of molecules with important functions such as adherence, invasion, signalling and interaction with the host immune system or environment. in gram-positive bacteria, many surface proteins of the "lpxtg" family are anchored to the peptidoglycan (pg) by an enzyme named sortase. while this anchoring mechanism has been clearly deciphered, less is known about the spatial organization of cell wall-anchored proteins in the bacterial envelope. h ... | 2016 | 27025379 |
| screening of the enterocin-encoding genes and antimicrobial activity in enterococcus species. | in the current study, a total of 135 enterococci strains from different sources were screened for the presence of the enterocin-encoding genes enta, entp, entb, entl50a, and entl50b. the enterocin genes were present at different frequencies, with enta occurring the most frequently, followed by entp and entb; entl50a and l50b were not detected. the occurrence of single enterocin genes was higher than the occurrence of multiple enterocin gene combinations. the 80 isolates that harbor at least one ... | 2016 | 26907753 |
| tigecycline resistance in clinical isolates of enterococcus faecium is mediated by an upregulation of plasmid-encoded tetracycline determinants tet(l) and tet(m). | tigecycline represents one of the last-line therapeutics to combat multidrug-resistant bacterial pathogens, including vre and mrsa. the german national reference centre for staphylococci and enterococci has received 73 tigecycline-resistant enterococcus faecium and enterococcus faecalis isolates in recent years. the precise mechanism of how enterococci become resistant to tigecycline remains undetermined. this study documents an analysis of the role of efflux pumps in tigecycline resistance in c ... | 2016 | 26682961 |
| transfer, composition and technological characterization of the lactic acid bacterial populations of the wooden vats used to produce traditional stretched cheeses. | the biofilms of 12 wooden vats used for the production of the traditional stretched cheeses caciocavallo palermitano and pdo vastedda della valle del belìce were investigated. salmonella spp. and listeria monocytogenes were never detected. total coliforms were at low numbers with escherichia coli found only in three vats. coagulase-positive staphylococci (cps) were below the enumeration limit, whereas lactic acid bacteria (lab) dominated the surfaces of all vats. in general, the dominance was sh ... | 2015 | 26338114 |
| characterization of lactobacillus fermentum pl9988 isolated from healthy elderly korean in a longevity village. | in this work, we wanted to develop a probiotic from famous longevity villages in korea. we visited eight longevity villages in korea to collect fecal samples from healthy adults who were aged above 80 years and had regular bowel movements, and isolated lactic-acid-producing bacteria from the samples. isolated colonies that appeared on mrs agar containing bromophenol blue were identified by means of 16s rrna sequencing, and 102 of the isolates were identified as lactic-acid-producing bacteria (18 ... | 2015 | 26095384 |
| probiotic features of lactobacillus strains isolated from ragusano and pecorino siciliano cheeses. | in the present study 177 lactobacillus spp. strains, isolated from ragusano and pecorino siciliano cheeses, were in vitro screened for probiotic traits, and their characteristics were compared to those of lactobacillus rhamnosus gg, commercial strain. based on acidic and bile salt resistance, thirteen lactobacillus strains were selected. the multiplex-pcr application revealed that nine strains belonged to l. rhamnosus species and four to lactobacillus paracasei species. all selected strains were ... | 2015 | 25998823 |
| purification and characterization of a bacteriocin from an oenological strain of leuconostoc mesenteroides subsp. cremoris. | malolactic fermentation (mlf), which improves organoleptic properties and biologic stability of some wines, may cause wine spoilage if uncontrolled. bacteriocins were reported as efficient preservatives to control mlf through their bactericidal effect on malolactic bacteria. leuconostoc mesenteroides subsp. cremoris w3 isolated from wine produces an inhibitory substance that is bactericidal against malolactic bacteria in model wine medium. treatment of the culture supernatant of strain w3 with p ... | 2016 | 25837975 |
| antimicrobial action of oleanolic acid on listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. | this study investigated the antimicrobial action of oleanolic acid against listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. to determine the cytotoxicity of oleanolic acid, hep-2 cells were incubated with oleanolic acid at 37°c. mics (minimal inhibition concentrations) for l. monocytogenes, e. faecium, and e. faecalis were determined using two-fold microdilutions of oleanolic acid, and bacterial cell viability was then assessed by exposing the bacteria to oleanolic acid a ... | 2015 | 25756202 |
| behavior of listeria monocytogenes in a multi-species biofilm with enterococcus faecalis and enterococcus faecium and control through sanitation procedures. | the formation of mono-species biofilm (listeria monocytogenes) and multi-species biofilms (enterococcus faecium, enterococcus faecalis, and l. monocytogenes) was evaluated. in addition, the effectiveness of sanitation procedures for the control of the multi-species biofilm also was evaluated. the biofilms were grown on stainless steel coupons at various incubation temperatures (7, 25 and 39°c) and contact times (0, 1, 2, 4, 6 and 8 days). in all tests, at 7°c, the microbial counts were below 0.4 ... | 2015 | 25655573 |
| extraction, characterization and antimicrobial activity of sulfated polysaccharides from fish skins. | sulfated polysaccharides were extracted from gray triggerfish (gtsp) and smooth hound (shsp) skins. their chemical and physical characteristics were determined using x-ray diffraction and infrared spectroscopic analysis. the antibacterial activities of gtsp and shsp against listeria monocytogenes (atcc 43251), staphylococcus aureus (atcc 25923), enterococcus faecalis (atcc 29212), escherichia coli (atcc 25922), salmonella enterica (atcc 43972) and enterobacter sp were evaluated by determining cl ... | 2015 | 25647621 |
| detection of listeria monocytogenes with short peptide fragments from class iia bacteriocins as recognition elements. | we employed a direct peptide-bacteria binding assay to screen peptide fragments for high and specific binding to listeria monocytogenes. peptides were screened from a peptide array library synthesized on cellulose membrane. twenty four peptide fragments (each a 14-mer) were derived from three potent anti-listerial peptides, leucocin a, pediocin pa1, and curvacin a, that belong to class iia bacteriocins. fragment leu10 (geafsagvhrlang), derived from the c-terminal region of leucocin a, displayed ... | 2015 | 25548942 |
| bacteriocin protein bacl1 of enterococcus faecalis targets cell division loci and specifically recognizes l-ala2-cross-bridged peptidoglycan. | bacteriocin 41 (bac41) is produced from clinical isolates of enterococcus faecalis and consists of two extracellular proteins, bacl1 and baca. we previously reported that bacl1 protein (595 amino acids, 64.5 kda) is a bacteriolytic peptidoglycan d-isoglutamyl-l-lysine endopeptidase that induces cell lysis of e. faecalis when an accessory factor, baca, is copresent. however, the target of bacl1 remains unknown. in this study, we investigated the targeting specificity of bacl1. fluorescence micros ... | 2015 | 25368300 |
| selection of potential probiotic enterococcus faecium isolated from portuguese fermented food. | four enterococcus faecium strains isolated from fermented products were evaluated for potential use as probiotic strains. in addition to efaafm gene, commonly found in e. faecium food isolates, none of the isolates possessed virulence genes and none had positive reactions for the production of tyramine, histamine, putrescine and cadaverine in the screening medium used. all of these four isolates proved to be resistant to 65 °c. e. faecium 119 did not show antimicrobial activity against any of th ... | 2014 | 25268323 |
| control of listeria monocytogenes in fresh cheese using protective lactic acid bacteria. | in the past years, there has been a particular focus on the application of bacteriocins produced by lactic acid bacteria (lab) in controlling the growth of pathogenic bacteria in foods. the aim of this study was to select lab strains with antimicrobial activity, previously isolated from a traditional azorean artisanal cheese (pico cheese), in order to identify those with the greatest potential in reducing listeria monocytogenes in fresh cheese. eight bacteriocin producer strains identified as la ... | 2014 | 25222327 |
| the cyclic dinucleotide c-di-amp is an allosteric regulator of metabolic enzyme function. | cyclic di-adenosine monophosphate (c-di-amp) is a broadly conserved second messenger required for bacterial growth and infection. however, the molecular mechanisms of c-di-amp signaling are still poorly understood. using a chemical proteomics screen for c-di-amp-interacting proteins in the pathogen listeria monocytogenes, we identified several broadly conserved protein receptors, including the central metabolic enzyme pyruvate carboxylase (lmpc). biochemical and crystallographic studies of the l ... | 2014 | 25215494 |
| [neonatal bacteremia--frequency, spectrum of organisms and correlation between clinical symptoms and laboratory data for early onset sepsis]. | improved obstetrical management and evidence-based peripartum antimicrobial therapy in the last decades reduce the frequency of early-onset neonatal sepsis (eons) and improve the outcome. the spectrum of the microrganisms is different according to regions, countries and periods of time. appropriate diagnosis and treatment improve the outcome. however dissociation often occurs between clinical manifestation, laboratory and microbiological findings. | 2014 | 25098106 |
| comparative genomic and morphological analyses of listeria phages isolated from farm environments. | the genus listeria is ubiquitous in the environment and includes the globally important food-borne pathogen listeria monocytogenes. while the genomic diversity of listeria has been well studied, considerably less is known about the genomic and morphological diversity of listeria bacteriophages. in this study, we sequenced and analyzed the genomes of 14 listeria phages isolated mostly from new york dairy farm environments as well as one related enterococcus faecalis phage to obtain information on ... | 2014 | 24837381 |
| antibacterial properties and mode of action of new triaryl butene citrate compounds. | the aim of this study was to evaluate the antibacterial activity of newly synthesized triaryl butene analogues of tamoxifen. several compounds were synthesized and converted to citrate salts to ensure greater solubility. four compounds showed significant antibacterial activity at micromolar concentrations against gram-positive and gram-negative foodborne pathogens including listeria monocytogenes, listeria ivanovii, enterococcus faecalis, staphylococcus aureus and escherichia coli. two compounds ... | 2014 | 24602786 |
| antimicrobial susceptibility and antibiotic resistance gene transfer analysis of foodborne, clinical, and environmental listeria spp. isolates including listeria monocytogenes. | the aims of this study were to assess antibiotic resistance pheno- and genotypes in foodborne, clinical, and environmental listeria isolates, as well as to elucidate the horizontal gene transfer potential of detected resistance genes. a small fraction of in total 524 listeria spp. isolates (3.1%) displayed acquired antibiotic resistance mainly to tetracycline (n = 11), but also to clindamycin (n = 4) and trimethoprim (n = 3), which was genotypically confirmed. in two cases, a tetracycline resist ... | 2014 | 24382842 |
| pathways and roles of wall teichoic acid glycosylation in staphylococcus aureus. | the thick peptidoglycan layers of gram-positive bacteria are connected to polyanionic glycopolymers called wall teichoic acids (wta). pathogens such as staphylococcus aureus, listeria monocytogenes, or enterococcus faecalis produce wta with diverse, usually strain-specific structure. extensive studies on s. aureus wta mutants revealed important functions of wta in cell division, growth, morphogenesis, resistance to antimicrobials, and interaction with host or phages. while most of the s. aureus ... | 2014 | 24365646 |
| screening for polyphenols, antioxidant and antimicrobial activitiesof extracts from eleven helianthemum taxa (cistaceae) used in folk medicine in south-eastern spain. | the helianthemum genus contains approximately one hundred taxa. some of them are important medicinal plants used in several countries for many different purposes. however, studies addressing the phytochemistry of many of these species or their biological activities are currently nonexistent. | 2013 | 23615124 |
| cd137 expressed on neutrophils plays dual roles in antibacterial responses against gram-positive and gram-negative bacterial infections. | severe sepsis and septic shock caused mainly by bacterial infections are life-threatening conditions that urge the development of novel therapies. however, host responses to and pathophysiology of sepsis have not been clearly understood, which remains a major obstacle for the development of effective therapeutics. recently, we have shown that stimulation of a costimulatory molecule, cd137, enhanced survival of mice infected with the gram-positive (g(+)) intracellular bacterium listeria monocytog ... | 2013 | 23545301 |
| tn6198, a novel transposon containing the trimethoprim resistance gene dfrg embedded into a tn916 element in listeria monocytogenes. | to characterize tn6198, a novel conjugative transposon from the clinical listeria monocytogenes strain tth-2007, which contains the tetracycline and trimethoprim resistance genes tet(m) and dfrg, respectively, and to assess its transferability in vitro and in situ. | 2013 | 23344576 |
| comparative kinetic analysis of glycerol 3-phosphate cytidylyltransferase from enterococcus faecalis and listeria monocytogenes. | glycerol 3-phosphate cytidylyltransferase (gct) is an enzyme central to the synthesis of teichoic acids, components of the cell wall in gram positive bacteria. catalysis by gct from enterococcus faecalis and listeria monocytogenes has been investigated and catalytic properties compared. | 2012 | 23111733 |
| inhibition of listeria monocytogenes by enterococcus mundtii isolated from soil. | two bacterial isolates with inhibitory activity against listeria monocytogenes and enterococcus faecalis were obtained from soil. genotypic and phenotypic characterization identified them as enterococcus mundtii, a species whose ability to compete with l. monocytogenes is relatively unexplored compared to other members of the genus. the thermal stability of the inhibitory factor and its sensitivity to proteolytic enzymes indicate that it is most likely a bacteriocin. both isolates grew at compar ... | 2012 | 22986201 |
| stimulation of cadaverine production by foodborne pathogens in the presence of lactobacillus, lactococcus, and streptococcus spp. | the effect of lactobacillus plantarum (fi8595), lactococcus lactis subsp. cremoris mg 1363), lactococcus lactis subsp. lactis (il 1403), and streptococcus thermophilus on cadaverine and other biogenic amine production by foodborne pathogens was investigated lysine decarboxylase broth. both of lactic acid bacteria and foodborne pathogens used (especially staphylococcus aureus, e. coli, lc. lactis subsp. lactis and lb. plantarum) had an ability to convert aminoacids into biogenic amine. the conver ... | 2012 | 22853653 |
| application of bacteriocinogenic enterococcus mundtii crl35 and enterococcus faecium st88ch in the control of listeria monocytogenes in fresh minas cheese. | several strains of enterococcus spp. are capable of producing bacteriocins with antimicrobial activity against important bacterial pathogens in dairy products. in this study, the bacteriocins produced by two enterococcus strains (enterococcus mundtii crl35 and enterococcus faecium st88ch), isolated from cheeses, were characterized and tested for their capability to control growth of listeria monocytogenes 426 in experimentally contaminated fresh minas cheese during refrigerated storage. both str ... | 2012 | 22850372 |
| antimicrobial activity of wild mushroom extracts against clinical isolates resistant to different antibiotics. | this work aimed to screen the antimicrobial activity of aqueous methanolic extracts of 13 mushroom species, collected in bragança, against several clinical isolates obtained in hospital center of trás-os-montes and alto douro, portugal. | 2012 | 22621239 |
| first study on antimicriobial activity and synergy between isothiocyanates and antibiotics against selected gram-negative and gram-positive pathogenic bacteria from clinical and animal source. | the emergence of new diseases and the resurgence of several infections that were controlled in the past, associated with recent increase of bacterial resistance have created the necessity for more studies towards to the development of new antimicrobials and new treatment strategies. the aim of the present study was to evaluate the in vitro synergy between different classes of important glucosinolates hydrolysis products-isothiocyanates with antibiotics (gentamycin and vancomycin), against import ... | 2012 | 22530889 |
| isolation and lytic activity of the listeria bacteriophage endolysin lysz5 against listeria monocytogenes in soya milk. | the endolysin gene (lysz5) from the genome of the listeria monocytogenes phage fwllm3 was cloned in escherichia coli and characterized. comparative sequence analysis revealed that lysz5 resembled the murein hydrolase ply511 encoded by l. monocytogenes phage a511. the encoded protein lysz5 had a predicted molecular mass of 35.8 kda and was expressed in e. coli as an n-terminal fusion protein of 41.5 kda. addition of purified fusion protein to lawns of indicator bacteria showed that lysz5 could ly ... | 2012 | 22475951 |
| comparison of swab transport media for recovery of listeria monocytogenes from environmental samples. | environmental monitoring is recognized as an important strategy for controlling listeria monocytogenes in food processing facilities. samples are taken by swabbing environmental surfaces, and the swabs are immersed in a medium for transport to the laboratory. in this study, buffered peptone water (bpw), dey-engley neutralizing broth (de), neutralizing buffer (nb), letheen broth (le), and newly described mcc buffer (mcc) were evaluated as transport media for recovery of sanitizer-stressed l. mono ... | 2012 | 22410235 |
| inhibition of listeria monocytogenes on ready-to-eat meats using bacteriocin mixtures based on mode-of-action. | bacteriocin-producing (bac⁺) lactic acid bacteria (lab) comprising selected strains of lactobacillus curvatus, lactococcus lactis, pediococcus acidilactici, and enterococcus faecium and thailandicus were examined for inhibition of listeria monocytogenes during hotdog challenge studies. the bac⁺ strains, or their cell-free supernatants (cfs), were grouped according to mode-of-action (moa) as determined from prior studies. making a mixture of as many moas as possible is a practical way to obtain a ... | 2017 | 28335414 |
| determination of antibacterial and technological properties of vaginal lactobacilli for their potential application in dairy products. | functional foods could differently affect human health in relation to the gender. recent studies have highlighted the anti-candida and anti-chlamydia activities of some lactobacillus strains isolated from the vagina of healthy women. considering these important beneficial activities on women's health, the preparation of functional food containing active vaginal lactobacilli can represent a great scientific challenge for the female gender. in this context, the aim of this work was to study some f ... | 2017 | 28223974 |
| enhanced control of listeria monocytogenes by enterococcus faecium ke82, a multiple enterocin-producing strain, in different milk environments. | enterococcus faecium ke82, isolated from traditional greek graviera cheese, was identified in pure broth cultures in vitro as a multiple enterocin-producing bacterial strain possessing the structural enta, entb, and entp enterocin genes. e. faecium ke82 was further assessed for in situ antilisterial activity in raw milk (rm) and commercially thermized milk (tm; 63°c for 30 s) in the presence of the indigenous microbiota and in sterile raw milk (srm; 121°c for 5 min) with or without the addition ... | 2017 | 28221890 |
| growth interactions and antilisterial effects of the bacteriocinogenic lactococcus lactis subsp. cremoris m104 and enterococcus faecium ke82 strains in thermized milk in the presence or absence of a commercial starter culture. | traditional greek cheeses are often produced from thermized milk (tm) with the use of commercial starter cultures (cscs), which may not inhibit growth of listeria monocytogenes completely. therefore, this study evaluated the behavior of an artificial l. monocytogenes contamination in commercially tm (63 °c; 30 s) inoculated with a csc plus lactococcus lactis subsp. lactis m104 and/or enterococcus faecium ke82, two indigenous strains producing nisin a and enterocin a and b, respectively. inoculat ... | 2017 | 28213019 |
| characterization of class iia bacteriocin resistance in enterococcus faecium. | vancomycin-resistant enterococci, particularly resistant enterococcus faecium, pose an escalating threat in nosocomial environments because of their innate resistance to many antibiotics, including vancomycin, a treatment of last resort. many class iia bacteriocins strongly target these enterococci and may offer a potential alternative for the management of this pathogen. however, e. faecium's resistance to these peptides remains relatively uncharacterized. here, we explored the development of r ... | 2017 | 28115354 |
| antibiotic susceptibility, antibacterial activity and characterisation of enterococcus faecium strains isolated from breast milk. | enterococci, which have useful biotechnological applications, produce bacteriocins, including those that exert anti-listerial activity. the present study aimed to determine the antibiotic susceptibility patterns and antimicrobial activity of enterococcus faecium strains isolated from human breast milk. the strains were identified using carbohydrate fermentation tests and ribotyping. subsequently, the antibacterial activity of the isolates was investigated, and the quantities of lactic acid and h ... | 2016 | 27602088 |
| purification of a novel bacteriocin-like inhibitory substance produced by enterococcus faecium icis 8 and characterization of its mode of action. | the aim of this work was to purify and characterize a bacteriocin-like antimicrobial substance produced by an antagonistic active strain of enterococcus faecium. | 2016 | 27575914 |
| core-shell nanorod columnar array combined with gold nanoplate-nanosphere assemblies enable powerful in situ sers detection of bacteria. | development of a label-free ultrasensitive nanosensor for detection of bacteria is presented. sensitive assay for gram-positive bacteria was achieved via electrostatic attraction-guided plasmonic bifacial superstructure/bacteria/columnar array assembled in one step. dynamic optical hotspots were formed in the hybridized nanoassembly under wet-dry critical state amplifying efficiently the weak vibrational modes of three representative food-borne gram-positive bacteria, that is, staphylococcus xyl ... | 2016 | 27574829 |
| pediocin sa-1: a selective bacteriocin for controlling listeria monocytogenes in maize silages. | in this study, we assessed the potential as silage additive of a bacteriocin produced by pediococcus acidilactici northern regional research laboratory (nrrl) b-5627 (pediocin sa-1). maize was inoculated either with a bacterial starter alone (i) or in combination with the bacteriocin (ip), and untreated silage served as control. we monitored the products of fermentation (ethanol, and lactic and acetic acids), the microbial population, and the presence of the indicator strain listeria monocytogen ... | 2016 | 27522409 |
| evaluation of technological properties of enterococcus faecium cect 8849, a strain isolated from human milk, for the dairy industry. | in this work, a variety of biochemical properties of enterococcus faecium cect 8849, which had been isolated from breast milk, were analyzed. its acidifying capacity and proteolytic activity were low but, in contrast, remarkable peptidase and esterase activities were observed. ethanol and 3-hydroxy-2-butanone were the most abundant volatile compounds found in experimental model cheese manufactured with e. faecium cect 8849. this strain inhibited the growth of several listeria monocytogenes and l ... | 2016 | 27216614 |
| inactivation of salmonella, listeria monocytogenes and enterococcus faecium nrrl b-2354 in a selection of low moisture foods. | the aims of this study were to obtain data on survival and heat resistance of cocktails of salmonella, listeria monocytogenes and the surrogate enterococcus faecium (nrrl b-2354) in four low moisture foods (confectionery formulation, chicken meat powder, pet food and savoury seasoning) during storage before processing. inoculated samples were stored at 16°c and cell viability examined at day 0, 3, 7 and 21. at each time point, the heat resistance at 80°c was determined. the purpose was to determ ... | 2016 | 27174678 |
| analysis of the impact of rosuvastatin on bacterial mevalonate production using a uplc-mass spectrometry approach. | statins are widely prescribed cholesterol-lowering medications and act through inhibition of the human enzyme 3-methylglutaryl coenzyme a reductase (hmg-r) which produces mevalonate (mval), a key substrate for cholesterol biosynthesis. some important microbial species also express an isoform of hmg-r; however, the nature of the interaction between statins and bacteria is currently unclear and studies would benefit from protocols to quantify mval in complex microbial environments. the objective o ... | 2016 | 26960292 |
| transfer of antibiotic resistance from enterococcus faecium of fermented meat origin to listeria monocytogenes and listeria innocua. | listeria monocytogenes is an important foodborne pathogen that can cause infection in children, pregnant women, the immunocompromised and the elderly. antibiotic resistance in this species would represent a significant public health problem since the organism has a high fatality/case ratio and resistance may contribute to failure of therapeutic treatment. this study was designed to explore whether the in vitro transferability of antibiotic resistance from enterococci to listeria spp. could occur ... | 2016 | 26854329 |
| antilisterial effect of bacteriocin sh01, obtained from enterococcus faecium sh01, in ground beef. | from the previous study, enterococcus faecium sh01 was isolated from mukeunji, an over-ripened kimchi, and it produced bacteriocin sh01. bacteriocin sh01 showed an inhibitory effect against listeria monocytogenes atcc 19111, a bacterial strain causing human listeriosis. crude bacteriocin sh01 was purified by ammonium sulfate precipitation and its inhibitory activity at two concentrations (500 and 1,000 au/g) against listeria monocytogenes atcc 19111 was investigated in ground beef at increasing ... | 2015 | 26761830 |
| a novel enterocin t1 with anti-pseudomonas activity produced by enterococcus faecium t1 from chinese tibet cheese. | an enterocin-producing enterococcus faecium t1 was isolated from chinese tibet cheese. the enterocin was purified by sp-sepharose and reversed phase hplc. it was identified as unique from other reported bacteriocins based on molecular weight (4629 da) and amino acid compositions; therefore it was subsequently named enterocin t1. enterocin t1 was stable at 80-100 °c and over a wide ph range, ph 3.0-10.0. protease sensitivity was observed to trypsin, pepsin, papain, proteinase k, and pronase e. im ... | 2016 | 26745981 |
| antimicrobial and antioxidant activities of enterococcus species isolated from meat and dairy products. | lactic acid bacteria (lab) have an important role in a great variety of fermented foods. in addition to their contribution to sensory characteristics, they enhance food preservation and can be used as probiotics. in this study, the antimicrobial and antioxidant activities of culture supernatants and cell free extracts of 16 lab isolated from meat and dairy products were investigated. the bacterial were identified by 16s rrna sequencing. genbank blast analysis revealed that all the isolates belon ... | 2015 | 26675908 |
| [biological activity of antimicrobial peptides of enterococcus faecium]. | isolate bacteriocins from enterococcus faecium metabolites and characterize their effect on cells of gram positive (listeria monocytogenes) and gram negative (escherichia coli) bacteria. | 2015 | 26470413 |
| in situ production of pediocin pa-1 like bacteriocin by different genera of lactic acid bacteria in soymilk fermentation and evaluation of sensory properties of the fermented soy curd. | the lactic acid bacteria (lab) are found to produce bacteriocins with enhanced nutritive properties in the fermented foods. in the present study, the ability of lab cultures (pediococcus acidilactici ncim 5424, enterococcus faecium ncim 5423 and lactobacillus plantarum acr2) to produce pediocin pa-1 like bacteriocin was evaluated during soymilk fermentation. the isolates e. faecium ncim 5423 and lb. plantarum acr2 were able to produce bacteriocin as well as ferment soymilk within 6 h of incubati ... | 2014 | 26396327 |
| antimicrobial activity and the presence of virulence factors and bacteriocin structural genes in enterococcus faecium cm33 isolated from ewe colostrum. | screening of lactic acid bacteria (lab) isolated from ewe colostrum led to the identification and isolation of enterococcus faecium cm33 with interesting features like high survival rates under acidic or bile salts condition, high tolerance for the simulated gastrointestinal condition, and high adhesive potential to caco-2 cells. according the inhibition of pathogen adhesion test results, this strain can reduce more than 50% adhesion capacity of escherichia coli, shigella flexneri, klebsiella pn ... | 2015 | 26284059 |
| experimental studies of microbial populations and incidence of zoonotic pathogens in the faeces of red deer (cervus elaphus). | wild animals can serve as hosts, amplifiers or reservoirs for various zoonotic diseases. most species of deer in highly fragmented agricultural landscapes, search out maximum cover from intrusive human activity. hence, the likelihood of zoonosis transmission is likely to increase the more humans and wildlife interact. in our study, we conducted a comparative analysis of bacteria isolated from the faeces of red deer (cervus elaphus) living in their natural environment in south-western poland and ... | 2015 | 26222832 |
| a microplate growth inhibition assay for screening bacteriocins against listeria monocytogenes to differentiate their mode-of-action. | lactic acid bacteria (lab) have historically been used in food fermentations to preserve foods and are generally-recognized-as-safe (gras) by the fda for use as food ingredients. in addition to lactic acid; some strains also produce bacteriocins that have been proposed for use as food preservatives. in this study we examined the inhibition of listeria monocytogenes 39-2 by neutralized and non-neutralized bacteriocin preparations (bac+ preps) produced by lactobacillus curvatus fs47; lb. curvatus ... | 2015 | 26111195 |
| in vitro protective effect of lactic acid bacteria on listeria monocytogenes adhesion and invasion of caco-2 cells. | the adhesion of listeria monocytogenes to intestinal endothelial cells is a crucial step in the infection process, which is not well understood. in this study, we evaluated the potential ability of bacteriocin-producing enterococcus faecium, leuconostoc mesenteroides and lactobacillus sakei strains to prevent the adhesion and invasion of eukaryotic cells by ten different l. monocytogenes isolates. the results showed that e. faecium 130 co-cultured with l. monocytogenes was the most effective in ... | 2015 | 25672897 |
| antibacterial activity of enterococcus faecium derived from koopeh cheese against listeria monocytogenes in probiotic ultra-filtrated cheese. | viability of probiotic bacteria in food during maintenance and time of consuming in food has become a challenge in food hygiene and technology and is important for representing their beneficial health effects. the aim of this study was to determine the survival of probiotic enterococcus faecium derived from koopeh cheese added to industrial iranian ultra-filtrated (uf) cheese and screening for antimicrobial activity of enterococcus faecium against listeria monocytogenes during two months of chee ... | 2014 | 25568714 |