| molecular characterization of encephalitic bovine listeriosis from southern brazil. | reports of bovine listeriosis in brazil are uncommon, being restricted to citations within retrospective studies, resulting in scarce documented information of this important disease of cattle. this manuscript describes the molecular findings associated with spontaneous encephalitic listeriosis in two steers from distinct herds within the state of paraná, southern brazil. both animals demonstrated altered consciousness suggestive of brain stem dysfunctions and died a few days after the initial o ... | 2014 | 23832703 |
| histophilus somni-induced infections in cattle from southern brazil. | the sudden death of three calves, one diarrheic calf, and one aborted fetus from four farms in southern brazil was investigated. two histophilus somni-associated syndromes were identified: systemic histophilosis (n = 4) and abortion (n = 1). the principal pathological findings included vasculitis, meningoencephalitis with thrombosis, necrotizing myocarditis, renal infarctions, hepatic abscesses, and bronchopneumonia. pcr assays were used to amplify specific amplicons of the ovine herpesvirus 2, ... | 2013 | 23526124 |
| atypical cerebral listeriosis associated with listeria innocua in a beef bull. | natural infections of cattle associated with listeria innocua have not been reported. this report describes the first case of cerebral listeriosis in a bull due to listeria innocua. the animal presented neurological signs characterized by weakness, incoordination and recumbency. histopathologic evaluation of brain tissue revealed multifocal microabscesses, perivascular lymphocytic cuffing, vasculitis, oedema and haemorrhages. all lesions extended from the medulla oblongata to the basal nuclei/pa ... | 2013 | 22898534 |
| [seroprevalance of brucellosis, listeriosis and toxoplasmosis in cattle in adana province of turkey]. | this study was conducted to identify the seroprevalance of diseases which are zoonotic and responsible from abortion such as toxoplasmosis, listeriosis and brucellosis in holstein crossbred cattle of different age and sex in adana province, between 2008 april-september. | 2014 | 25016114 |
| [identification of zoonotic bacterial pathogens by the maldi tof ms method]. | to verify whether the maldi tof ms method can be used for rapid identification of selected zoonotic bacterial pathogens isolated from various types of materials in the real conditions of routine laboratory work. | 2015 | 26312374 |
| invasive escherichia coli vaccines expressing brucella melitensis outer membrane proteins 31 or 16 or periplasmic protein bp26 confer protection in mice challenged with b. melitensis. | because of the serious economic and medical consequences of brucellosis, efforts are to prevent infection of domestic animals through vaccines. many disadvantages are associated with the current brucella melitensis rev.1 vaccine prompting development of alternative vaccines and delivery. escherichia coli (dh5α) was engineered to express a plasmid containing the inv gene from yersinia pseudotuberculosis and the hly gene from listeria monocytogenes. these recombinant invasive e. coli expressing b. ... | 2012 | 22546330 |
| inhibition of bacterial biofilm formation and swarming motility by a small synthetic cationic peptide. | biofilms cause up to 80% of infections and are difficult to treat due to their substantial multidrug resistance compared to their planktonic counterparts. based on the observation that human peptide ll-37 is able to block biofilm formation at concentrations below its mic, we screened for small peptides with antibiofilm activity and identified novel synthetic cationic peptide 1037 of only 9 amino acids in length. peptide 1037 had very weak antimicrobial activity, but at 1/30th the mic the peptide ... | 2012 | 22354291 |
| [regularities of the ubiquitous polyhostal microorganisms selection by the example of three taxa]. | the investigation of the bacterial populations' heterogeneity contributes to the control of natural foci, causative agents of nosocomial infections, to the analysis of the microbial evolution. multilocus sequence typing (mlst) was employed for the analysis of the diversity and features of the distribution of polyhostal ubiquitous microorganisms of the genera burkholderia, leptospira, and listeria, which belong to three bacterial phyla: proteobacteria, spirochaetes, and firmicutes. according to t ... | 2015 | 26107896 |
| zoonotic public health hazards in backyard chickens. | backyard poultry has become increasingly popular in industrialized countries. in addition to keeping chickens for eggs and meat, owners often treat the birds as pets. however, several pathogenic enteric bacteria have the potential for zoonotic transmission from poultry to humans but very little is known about the occurrence of zoonotic pathogens in backyard flocks. the occurrence and the antimicrobial resistance of salmonella enterica, campylobacter spp., listeria monocytogenes and enteropathoge ... | 2016 | 26752227 |
| rapid identification and classification of campylobacter spp. using laser optical scattering technology. | campylobacter jejuni and campylobacter coli are the two important species responsible for most of the campylobacter infections in humans. reliable isolation and detection of campylobacter spp. from food samples are challenging due to the interferences from complex food substances and the fastidious growth requirements of this organism. in this study, a novel biosensor-based detection called bardot (bacterial rapid detection using optical scattering technology) was developed for high-throughput s ... | 2015 | 25583335 |
| pathogenic microbiological baseline survey of pork carcasses in taiwan. | from 2004 to 2010, pork carcass swabs from state-inspected slaughter plants in taiwan were intermittently analyzed to determine the prevalence of selected pathogenic microorganisms associated with foodborne illness. the prevalences of staphylococcus aureus each year from 2006 to 2010 were 6.6, 10.8, 5.1, 6.4, and 7.4%, respectively, while those of listeria monocytogenes were 1.2% in 2004, 1.3% in 2005, and 3.5% in 2008. the prevalences of clostridium perfringens were 0.9% in 2004, 3.2% in 2005, ... | 2013 | 23726203 |
| a comparative analysis of the lyve-set phylogenomics pipeline for genomic epidemiology of foodborne pathogens. | modern epidemiology of foodborne bacterial pathogens in industrialized countries relies increasingly on whole genome sequencing (wgs) techniques. as opposed to profiling techniques such as pulsed-field gel electrophoresis, wgs requires a variety of computational methods. since 2013, united states agencies responsible for food safety including the cdc, fda, and usda, have been performing whole-genome sequencing (wgs) on all listeria monocytogenes found in clinical, food, and environmental samples ... | 2017 | 28348549 |
| knowledge gaps in host-parasite interaction preclude accurate assessment of meat-borne exposure to toxoplasma gondii. | toxoplasma gondii is recognized as a widely prevalent zoonotic parasite worldwide. although several studies clearly identified meat products as an important source of t. gondii infections in humans, quantitative understanding of the risk posed to humans through the food chain is surprisingly scant. while probabilistic risk assessments for pathogens such as campylobacter jejuni, listeria monocytogenes or escherichia coli have been well established, attempts to quantify the probability of human ex ... | 2016 | 28139250 |
| decay rates of zoonotic pathogens and viral surrogates in soils amended with biosolids and manures and comparison of qpcr and culture derived rates. | the purpose of this study was to establish inactivation decay constants of foodborne pathogens and coliphage in clay and sandy soils for future "downstream" analyses such as quantitative microbial risk analysis and to compare cultivation-dependent and -independent (e.g. qpcr) methods. | 2016 | 27585434 |
| efficacy of neutral electrolyzed water, quaternary ammonium and lactic acid-based solutions in controlling microbial contamination of food cutting boards using a manual spraying technique. | bactericidal activity of neutral electrolyzed water (new), quaternary ammonium (quat), and lactic acid-based solutions was investigated using a manual spraying technique against salmonella typhimurium, escherichia coli o157:h7, campylobacter jejuni, listeria monocytogenes and staphylococcus aureus that were inoculated onto the surface of scarred polypropylene and wooden food cutting boards. antimicrobial activity was also examined when using cutting boards in preparation of raw chopped beef, chi ... | 2016 | 27027449 |
| validation of a high-throughput immunobead array technique for multiplex detection of three foodborne pathogens in chicken products. | this study rigorously evaluated a previously developed immunobead array method to simultaneously detect three important foodborne pathogens, campylobacter jejuni, listeria monocytogenes, and salmonella spp., for its actual application in routine food testing. due to the limitation of the detection limit of the developed method, an enrichment step was included in this study by using campylobacter enrichment broth for c. jejuni and universal pre-enrichment broth for l. monocytogenes and salmonella ... | 2016 | 26950032 |
| sensitive detection of campylobacter jejuni using nanoparticles enhanced qcm sensor. | a quartz crystal microbalance (qcm) sensor platform was used to develop an immunosensor for the detection of food pathogen campylobacter jejuni. rabbit polyclonal antibodies and commercially available mouse monoclonal antibodies against c. jejuni were investigated to construct direct, sandwich and gold-nanoparticles (aunps) amplified sandwich assays. the performance of the qcm immunosensor developed using sandwich assay by utilising the rabbit polyclonal antibody as the capture antibody and conj ... | 2016 | 26649490 |
| pathogen loading from canada geese faeces in freshwater: potential risks to human health through recreational water exposure. | canada geese (branta canadensis) faeces have been shown to contain pathogenic protozoa and bacteria in numerous studies over the past 15 years. further, increases in both the canada geese populations and their ideal habitat requirements in the united states (us) translate to a greater presence of these human pathogens in public areas, such as recreational freshwater beaches. combining these factors, the potential health risk posed by canada geese faeces at freshwater beaches presents an emerging ... | 2016 | 26414207 |
| antibacterial activities of ankaferd hemostat (abs) on shiga toxin-producing escherichia coli and other pathogens significant in foodborne diseases. | ankaferd hemostat (ankaferd blood stopper®, abs)-induced pharmacological modulation of essential erythroid proteins can cause vital erythroid aggregation via acting on fibrinogen gamma. topical endoscopic abs application is effective in the controlling of gastrointestinal (gi) system hemorrhages and/or infected gi wounds. escherichia coli o157:h7, the predominant serotype of enterohemorrhagic e. coli, is a cause of both outbreaks and sporadic cases of hemorrhagic colitis. the aim of this study i ... | 2017 | 26377624 |
| application of bacteriophages in post-harvest control of human pathogenic and food spoiling bacteria. | bacteriophages have attracted great attention for application in food biopreservation. lytic bacteriophages specific for human pathogenic bacteria can be isolated from natural sources such as animal feces or industrial wastes where the target bacteria inhabit. lytic bacteriophages have been tested in different food systems for inactivation of main food-borne pathogens including listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, salmonella enterica, shigella spp., campylobac ... | 2016 | 26042353 |
| effectiveness of inactivation of foodborne pathogens during simulated home pan frying of steak, hamburger or meat strips. | in order to evaluate the effect of simulated home pan frying of raw meat and meat preparations of different animal species on the thermal inactivation of pathogens, the heat resistance (d-value) of three strains of campylobacter jejuni, escherichia coli o157:h7, salmonella spp., listeria monocytogenes and two strains of generic e. coli was validated in bhi and adjusted bhi (i.e. ph5.6 and 1.5% nacl) at 60°c. the d-values were obtained of the linear phase of the survivor curves created in ginafit ... | 2015 | 26004267 |
| effect of oxygen stress on growth and survival of clostridium perfringens, campylobacter jejuni, and listeria monocytogenes under different storage conditions. | this study investigated the growth and survival of three foodborne pathogens (clostridium perfringens, campylobacter jejuni, and listeria monocytogenes) in beef (7% fat) and nutrient broth under different oxygen levels. samples were tested under anoxic (<0.5%), microoxic (6 to 8%), and oxic (20%) conditions during storage at 7 °c for 14 days and at 22 °c for 5 days. two initial inoculum concentrations were used (1 and 2 log cfu per g of beef or per ml of broth). the results show that c. perfring ... | 2015 | 25836393 |
| antibiotic susceptibility and prevalence of foodborne pathogens in poultry meat in romania. | the occurrence of pathogenic strains in poultry meat is of growing concern in romania. another problem found on a global level is the continuous increase of antimicrobial resistance in bacteria isolated from food. this study aimed to evaluate the prevalence of pathogenic bacteria in poultry carcasses obtained in romania in 2012-2013 and to reveal the most prevalent patterns of antimicrobial resistance in the isolated strains. | 2015 | 25596569 |
| chemical composition, antibacterial and antioxidant activities of six essentials oils from the alliaceae family. | six essential oils (eos) from the alliaceae family, namely garlic (allium sativum), onion (allium cepa), leek (allium porrum), chinese chive (allium tuberosum), shallot (allium ascalonicum) and chive (allium schoenoprasum) were characterized by gc and gc-ms and evaluated for their functional food properties. antibacterial properties were tested on five food-borne pathogens: two gram-positive staphylococcus aureus (atcc 25923), listeria monocytogenes (atcc 19115) and three gram-negative salmonell ... | 2014 | 25470273 |
| detection of salmonella spp. by a loop-mediated isothermal amplification (lamp) method targeting bcfd gene. | in this study, we developed and validated a loop-mediated isothermal amplification (lamp) assay for salmonella detection targeting bcfd gene, a conserved fimbrial operon gene existing in salmonella. the salmonella lamp assay we developed successfully amplified 44 salmonella strains (14 standard strains and 30 clinical isolates), but none of 9 non-salmonella standard strains (proteus mirabilis, listeria monocytogenes, escherichia coli, pseudomonas aeruginosa, shigella flexneri, shigella sonnei, k ... | 2014 | 25199410 |
| physicochemical and antimicrobial properties of citral and quercetin incorporated kafirin-based bioactive films. | the aim of this study was to determine the physicochemical and antimicrobial properties of kafirin-based bioactive films incorporating the plant essential oil citral and the polyphenol quercetin. the addition of quercetin and citral both imparted a yellowish colour to the films. the tensile strength of films significantly decreased and elongation at break increased when citral was incorporated, whereas addition of quercetin did not alter these two film parameters. the rate of water vapour transm ... | 2015 | 25172719 |
| food safety in raw milk production: risk factors associated to bacterial dna contamination. | while human illness from milkborne pathogens may be linked to contamination of the product after pasteurization or improper pasteurization, such diseases are usually associated with consumption of raw milk or its by-products. molecular biology tools were applied to investigate contamination by listeria monocytogenes, salmonella spp., some pathogenic strains of escherichia coli, and campylobacter jejuni in 548 raw milk samples from 125 dairy farms established in two regions from southern brazil. ... | 2014 | 24715203 |
| estimation of infectious risks in residential populations exposed to airborne pathogens during center pivot irrigation of dairy wastewaters. | in the western united states where dairy wastewaters are commonly land applied, there are concerns over individuals being exposed to airborne pathogens. in response, a quantitative microbial risk assessment (qmra) was performed to estimate infectious risks after inhalation exposure of pathogens aerosolized during center pivot irrigation of diluted dairy wastewaters. the dispersion of pathogens (campylobacter jejuni, escherichia coli o157:h7, non-o157 e. coli, listeria monocytogenes, and salmonel ... | 2014 | 24697271 |
| reduction of microbiological risk in minced meat by a combination of natural antimicrobials. | responsibility for food safety must be taken through the entire food-production chain, to avoid consumer cross-contamination. the antimicrobial activities of an alpinia katsumadai seed extract and epigallocatechin gallate (egcg), and their combination, were evaluated against individual food-borne pathogenic strains of listeria monocytogenes, escherichia coli and campylobacter jejuni, individually and as a cocktail, in chicken-meat juice and sterile minced meat as food models, and in minced meat ... | 2014 | 24532379 |
| use of synthetic genes for cloning, production and functional expression of the bacteriocins enterocin a and bacteriocin e 50-52 by pichia pastoris and kluyveromyces lactis. | the use of synthetic genes may constitute a successful approach for the heterologous production and functional expression of bacterial antimicrobial peptides (bacteriocins) by recombinant yeasts. in this work, synthetic genes with adapted codon usage designed from the mature amino acid sequence of the bacteriocin enterocin a (enta), produced by enterococcus faecium t136, and the mature bacteriocin e 50-52 (bace50-52), produced by e. faecium nrrl b-32746, were synthesized. the synthetic enta and ... | 2014 | 24510220 |
| consumption of raw or unpasteurized milk and milk products by pregnant women and children. | sales of raw or unpasteurized milk and milk products are still legal in at least 30 states in the united states. raw milk and milk products from cows, goats, and sheep continue to be a source of bacterial infections attributable to a number of virulent pathogens, including listeria monocytogenes, campylobacter jejuni, salmonella species, brucella species, and escherichia coli o157. these infections can occur in both healthy and immunocompromised individuals, including older adults, infants, youn ... | 2014 | 24344105 |
| a modified molecular beacons-based multiplex real-time pcr assay for simultaneous detection of eight foodborne pathogens in a single reaction and its application. | foodborne disease outbreaks are often caused by one of the major pathogens. early identification of the causal pathogen is crucial for disease control and prevention. we describe a real-time polymerase chain reaction (rtpcr) assay that can identify, in a single reaction, up to eight common foodborne bacterial pathogens, including salmonella enterica subsp. enterica, listeria monocytogenes, escherichia coli o157, vibrio parahaemolyticus, v. vulnificus, campylobacter jejuni, enterobacter sakazakii ... | 2014 | 24328501 |
| four-year monitoring of foodborne pathogens in raw milk sold by vending machines in italy. | prevalence data were collected from official microbiological records monitoring four selected foodborne pathogens (salmonella, listeria monocytogenes, escherichia coli o157:h7, and campylobacter jejuni) in raw milk sold by self-service vending machines in seven italian regions (60,907 samples from 1,239 vending machines) from 2008 to 2011. data from samples analyzed by both culture-based and real-time pcr methods were collected in one region. one hundred raw milk consumers in four regions were i ... | 2013 | 24215694 |
| production and evaluation of the utility of novel phage display-derived peptide ligands to salmonella spp. for magnetic separation. | the objectives of this study were to produce salmonella-specific peptide ligands by phage display biopanning and evaluate their use for magnetic separation (ms). | 2013 | 23551658 |
| prevalence of campylobacter spp., salmonella spp., listeria monocytogenes, and hepatitis e virus in swine livers collected at an abattoir. | we investigated the prevalence of campylobacter spp., salmonella spp., listeria monocytogenes, and hepatitis e virus (hev) in swine liver. we collected swine livers from 110 pigs at an abattoir from september 2011 to march 2012 [corrected] . pathogens were detected in the liver samples of 19 (17.3%) pigs. campylobacter spp. were isolated from the liver samples of 14 (12.7%) pigs. in 10 of the 14 campylobacter-positive pigs, bacteria were present in the internal regions of the liver. salmonella s ... | 2013 | 23514917 |
| in vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic lactobacillus strains and interaction with foodborne zoonotic bacteria, especially campylobacter jejuni. | bacterial aggregation and/or adhesion are key factors for colonization of the digestive ecosystem and the ability of probiotic strains to exclude pathogens. in the present study, two probiotic strains, lactobacillus rhamnosus cncm-i-3698 and lactobacillus farciminis cncm-i-3699, were evaluated as viable or heat-killed forms and compared with probiotic reference lactobacillus strains (lb. rhamnosus gg and lb. farciminis cip 103136). the autoaggregation potential of both forms was higher than that ... | 2013 | 23329323 |
| prevalence and characterization of foodborne pathogens in dairy cattle in the eastern part of japan. | to investigate the prevalence and characterization of foodborne pathogens [campylobacter spp., shiga toxin-producing escherichia coli (stec), listeria monocytogenes and salmonella spp.] in dairy cows, rectal content grab samples were collected from 250 dairy cows reared on 25 dairy farms in eastern japan from december 2010 through february 2011. campylobacter jejuni was isolated from 106 (42%) cows on 23 (92%) farms, stec o157 from three cows on one farm, l. monocytogenes from three cows on anot ... | 2013 | 23208291 |
| gangliosides and sialic acid effects upon newborn pathogenic bacteria adhesion: an in vitro study. | the effect of the main gangliosides (gm(1), gm(3), gd(3)) and free sialic acid (neu5ac) upon the adhesion of pathogenic bacteria implicated in infant diarrhoea is assessed in vitro using the caco-2 cell line. concentrations of the bioactive compounds found in the bioaccessible (soluble) fraction of infant formula and human milk are employed. bacterial adhesion behaviour included enterotoxigenic escherichia coli (etec), enteropathogenic e.coli (epec), listeria monocytogenes, salmonella entericase ... | 2013 | 23122120 |
| behaviour of non-stressed and stressed listeria monocytogenes and campylobacter jejuni cells on fresh chicken burger meat packaged under modified atmosphere and inoculated with protective culture. | numerous investigations have provided evidence that chicken products are a source of listeria monocytogenes and campylobacter jejuni. different strategies applied in final products are needed to prevent consumers' contamination. in this work, the combination of modified atmosphere packaging (map) and protective culture to control the growth of freeze stressed and non-stressed l. monocytogenes and c. jejuni on fresh chicken meat burger was studied. meat burgers were inoculated with l. monocytogen ... | 2012 | 22831819 |
| campylobacter, salmonella, listeria monocytogenes, verotoxigenic escherichia coli, and escherichia coli prevalence, enumeration, and subtypes on retail chicken breasts with and without skin. | this study examined the prevalence, counts, and subtypes of campylobacter, salmonella, listeria monocytogenes, verotoxigenic escherichia coli (vtec), and e. coli on raw retail chicken breast with the skin on versus the skin off. from january to december 2007, 187 raw skin-on chicken breasts and 131 skin-off chicken breasts were collected from randomly selected retail grocery stores in the region of waterloo, ontario, canada. campylobacter isolates were recovered from a higher proportion of the s ... | 2012 | 22221353 |
| human α-defensin 6: a small peptide that self-assembles and protects the host by entangling microbes. | human α-defensin 6 (hd6) is a 32-residue cysteine-rich peptide that contributes to innate immunity by protecting the host at mucosal sites. this peptide is produced in small intestinal paneth cells, stored as an 81-residue precursor peptide named prohd6 in granules, and released into the lumen. one unusual feature of hd6 is that it lacks the broad-spectrum antimicrobial activity observed for other human α-defensins, including the paneth cell peptide human α-defensin 5 (hd5). hd6 exhibits unprece ... | 2017 | 28296382 |
| essential oils against pathogen and spoilage microorganisms of fruit juices: use of versatile antimicrobial delivery systems. | essential oils (eo) are increasingly used as natural antimicrobial compounds, however the effect of delivery system to enhance their antimicrobial activity has not been widely studied. limonene (0 to 10 μl/ml) was added to microbial suspensions (∼10(5) cfu/ml) of selected foodborne pathogens (listeria monocytogenes scott a, salmonella enterica typhimurium, escherichia coli and staphylococcus aureus), and spoilage microorganisms (lactobacillus plantarum, saccharomyces cerevisiae, and candida albi ... | 2017 | 28071802 |
| construction of antimicrobial peptide-drug combination networks from scientific literature based on a semi-automated curation workflow. | considerable research efforts are being invested in the development of novel antimicrobial therapies effective against the growing number of multi-drug resistant pathogens. notably, the combination of different agents is increasingly explored as means to exploit and improve individual agent actions while minimizing microorganism resistance. although there are several databases on antimicrobial agents, scientific literature is the primary source of information on experimental antimicrobial combin ... | 2016 | 28025336 |
| complete genome sequence of lactobacillus plantarum lz206, a potential probiotic strain with antimicrobial activity against food-borne pathogenic microorganisms. | lactobacilli strains have been considered as important candidates for manufacturing "natural food", due to their antimicrobial properties and generally regarded as safe (gras) status. lactobacillus plantarum lz206 is a potential probiotic strain isolated from raw cow milk, with antimicrobial activity against various pathogens, including gram-positive bacteria (staphylococcus aureus and listeria monocytogenes), gram-negtive bacteria (escherichia coli and salmonella enterica), and fungus candida a ... | 2016 | 27671696 |
| development of new 5-(chromene-3-yl)methylene-2,4-thiazolidinediones as antimicrobial agents. | in the context of the increasing phenomenon of microbial resistance to usual drugs, the development of new treatment strategies and new therapeutic protocols is a constant need. thiazolidinedione and chromone represent two important scaffolds in medicinal chemistry due to their large pharmacological applicability. | 2016 | 27004035 |
| bioactive compounds contents, antioxidant and antimicrobial activities during ripening of prunus persica l. varieties from the north west of tunisia. | bioactive molecules from fruits of four varieties of prunus persica at different stages of ripening (green, small orange, red) were studied. for example, contents on polyphenols (20.36mg gae/g fw) and flavonoids (0.764mg re/g fw) were high and varied according variety. the antioxidant activity, using four different tests (dpph radical scavenging activity, reducing power, β carotene bleaching system and tbars assay) showed that the variety chatos exhibited the highest antioxidant activity compari ... | 2016 | 26988472 |
| calcium ions tune the zinc-sequestering properties and antimicrobial activity of human s100a12. | human s100a12 is a host-defense protein expressed and released by neutrophils that contributes to innate immunity. apo s100a12 is a 21-kda antiparallel homodimer that harbors two ca(ii)-binding ef-hand domains per subunit and exhibits two his3asp motifs for chelating transition metal ions at the homodimer interface. in this work, we present results from metal-binding studies and microbiology assays designed to ascertain whether ca(ii) ions modulate the zn(ii)-binding properties of s100a12 and fu ... | 2016 | 26913170 |
| zebrafish: an animal model for research in veterinary medicine. | the zebrafish (danio rerio) has become known as an excellent model organism for studies of vertebrate biology, vertebrate genetics, embryonal development, diseases and drug screening. nevertheless, there is still lack of detailed reports about usage of the zebrafish as a model in veterinary medicine. comparing to other vertebrates, they can lay hundreds of eggs at weekly intervals, externally fertilized zebrafish embryos are accessible to observation and manipulation at all stages of their devel ... | 2015 | 26618602 |
| yeast diversity in a traditional french cheese "tomme d'orchies" reveals infrequent and frequent species with associated benefits. | this study is aimed at unrevealing the yeast diversity of handmade cheese, tomme d'orchies, produced and marketed in the north of france. a total of 185 yeast colonies were isolated from the surface and core of this cheese. from these, 80 morphologically different colonies were selected and subjected to rep-pcr analysis. the isolates were clustered into six distinct groups based on their dna fingerprints. from each group, at least 30% of isolates were selected and identified to species level by ... | 2015 | 26338133 |
| blood volume required for detection of low levels and ultralow levels of organisms responsible for neonatal bacteremia by use of bactec peds plus/f, plus aerobic/f medium, and the bd bactec fx system: an in vitro study. | we used an in vitro technique to investigate blood volumes required to detect bacteremia and fungemia with low concentrations of an organism. at 1 to 10 cfu/ml, escherichia coli, staphylococcus epidermidis, staphylococcus aureus, listeria monocytogenes, candida albicans, and candida parapsilosis isolates were detected in volumes as low as 0.5 ml. detection of streptococcus agalactiae and detection of bacteremia at <1 cfu/ml were unreliable. | 2015 | 26292299 |
| broad spectrum anti-microbial compounds producing bacteria from coast of qingdao bays. | anti-microbial resistance burden and hazard associated with chemical treatment of infections demanded for new anti-microbial natural products. marine associated microorganisms are the enormous source of bioactive compounds. in this study we have isolated 272 marine bacteria among them 136 (50%) were antagonistic to at least one of the four pathogenic strains listeria monocytogenes, vibrio cholerae, e. coli and s. aureus. only two strains exhibited antibacterial activity against all four test str ... | 2015 | 25730803 |
| acipensins - novel antimicrobial peptides from leukocytes of the russian sturgeon acipenser gueldenstaedtii. | antimicrobial peptides (amps) play an important role in the innate defense mechanisms in humans and animals. we have isolated and studied a set of antimicrobial peptides from leukocytes of the russian sturgeon acipenser gueldenstaedtii belonging to a subclass of chondrosteans, an ancient group of bony fish. structural analysis of the isolated peptides, designated as acipensins (ac), revealed in leukocytes of the russian sturgeon six novel peptides with molecular masses of 5336.2 da, 3803.0 da, 5 ... | 2014 | 25558400 |
| acute conditions caused by infectious aortitis. | infection of the aorta is rare but potentially very dangerous. under normal circumstances the aorta is very resistant to infections. following some afflictions, the infection can pass to the aorta from blood or the surrounding tissues. the authors present their 5-year experience with therapy of various types of infections of the abdominal aorta. | 2014 | 26798723 |
| in situ total aortic arch replacement for infected distal aortic arch aneurysms with penetrating atherosclerotic ulcer. | we present a series of patients who underwent in situ total aortic arch replacement for infected distal aortic arch aneurysms. | 2014 | 24607377 |
| preparation and antimicrobial action of three tryptic digested functional molecules of bovine lactoferrin. | lactoferrin is an 80 kda bilobal, iron binding glycoprotein which is primarily antimicrobial in nature. the hydrolysis of lactoferrin by various proteases in the gut produces several functional fragments of lactoferrin which have varying molecular sizes and properties. here, bovine lactoferrin has been hydrolyzed by trypsin, the major enzyme present in the gut, to produce three functional molecules of sizes approximately 21 kda, 38 kda and 45 kda. the molecules have been purified using ion excha ... | 2014 | 24595088 |
| effects of microgravity on the virulence of listeria monocytogenes, enterococcus faecalis, candida albicans, and methicillin-resistant staphylococcus aureus. | to evaluate effects of microgravity on virulence, we studied the ability of four common clinical pathogens--listeria monocytogenes, methicillin-resistant staphylococcus aureus (mrsa), enterococcus faecalis, and candida albicans--to kill wild type caenorhabditis elegans (c. elegans) nematodes at the larval and adult stages. simultaneous studies were performed utilizing spaceflight, clinorotation in a 2-d clinorotation device, and static ground controls. the feeding rate of worms for killed e. col ... | 2013 | 24283929 |
| the performance of 4 different supplements and 5 blood culture bottles types in detection of bacteria and candida spp. in simulated sterile body fluid cultures. | the purpose of this investigation was to evaluate the performance of 4 supplements: horse blood, fastidious organisms supplement (fos), haemin isovitalex albumine (hia), and brain heart infusion-haemin isovitalex albumine (bhi-hia) and 5 blood culture bottles: bactec mycosis ic/f, plus aerobic/f, peds plus/f from the bactec 9240 system, and bact/alert fa and bact/alert pf from the bact/alert 3d system, in detection of bacteria and candida spp. in simulated sterile body fluids other than blood mo ... | 2013 | 23867327 |
| impaired neutrophil function in 24p3 null mice contributes to enhanced susceptibility to bacterial infections. | lipocalin 24p3 (24p3) is a neutrophil secondary granule protein. 24p3 is also a siderocalin, which binds several bacterial siderophores. it was therefore proposed that synthesis and secretion of 24p3 by stimulated macrophages or release of 24p3 upon neutrophil degranulation sequesters iron-laden siderophores to attenuate bacterial growth. accordingly, 24p3-deficient mice are susceptible to bacterial pathogens for which siderophores would normally be chelated by 24p3. specific granule deficiency ... | 2013 | 23543755 |
| antimicrobial screening of some egyptian plants and active flavones from lagerstroemia indica leaves. | one hundred and twenty four plant extracts were evaluated for their antimicrobial activity against four pathogenic bacteria (staphylococcus aureus (atcc 8095), salmonella enteritides (atcc 13076), escherichia coli (atcc 25922), and listeria monocytogenes (atcc 15313)) and candida albicans yeast (atcc 10231) using the disk diffusion and broth microdilution methods. of the plant extracts, fourteen exhibited antimicrobial activity against two or more of the five microorganisms tested. only the meth ... | 2012 | 23006992 |
| production of human lactoferrin in animal milk. | genetic constructs containing the human lactoferrin (hlf) gene were created within a joint program of russian and belorussian scientists. using these constructs, transgenic mice were bred (the maximum hlf concentration in their milk was 160 g/l), and transgenic goats were also generated (up to 10 g/l hlf in their milk). experimental goatherds that produced hlf in their milk were also bred, and the recombinant hlf was found to be identical to the natural protein in its physical and chemical prope ... | 2012 | 22360490 |
| reduced growth of listeria monocytogenes in two model cheese microcosms is not associated with individual microbial strains. | two model antilisterial microbial communities consisting of two yeasts, two gram positive and two gram negative bacteria, and originating from livarot cheese smear were previously designed. they were used in the present study to analyse the impact of microbial population dynamics on growth of listeria monocytogenes in cheese microcosm. specific culture media and pcr primers were developed for simultaneous culture-dependent and real-time pcr quantification of strains belonging to marinomonas sp., ... | 2013 | 23122498 |
| identification and partial characterization of antilisterial compounds produced by dairy yeasts. | food-grade yeasts make significant contributions to flavor development in fermented foods. some yeast species also inhibit undesirable bacteria, yeasts and molds, apparently by producing antimicrobial compounds called mycocins. the aim of this study was to evaluate the ability of wild yeasts, isolated from raw milk and cheese in the quebec province area, to produce antilisterial compounds. based on an agar-membrane screening test, 22 of 95 isolates, namely one candida catenulata, one candida par ... | 2013 | 26782600 |
| determination of the total phenolic and anthocyanin contents and antimicrobial activity of viburnum opulus fruit juice. | viburnum opulus is a plant with fruits that are rich in biologically active substances, making it valuable to the food and pharmaceutical industries. here, we present our study of the total phenolic and anthocyanin contents and antimicrobial activity in the fruit juice of six v. opulus l. accessions. the cultivar 'krasnaya grozd' was notable for its exceptionally large amount of total phenolics, 1168 mg/100 g, with anthocyanins comprising 3-5 % of the total phenolic content. the evaluation of th ... | 2012 | 22865031 |
| the antimicrobial effects of wood-associated polyphenols on food pathogens and spoilage organisms. | the antimicrobial effects of the wood-associated polyphenolic compounds pinosylvin, pinosylvin monomethyl ether, astringin, piceatannol, isorhapontin, isorhapontigenin, cycloxme, dhimp, arx, and arxoh were assessed against both gram-negative (salmonella) and gram-positive bacteria (listeria monocytogenes, staphylococcus epidermidis, staphylococcus aureus) and yeasts (candida tropicalis, saccharomyces cerevisiae). particularly the stilbenes pinosylvin, its monomethyl ether and piceatannol demonst ... | 2013 | 23624538 |
| improving simultaneously the quality and safety of cooked and peeled shrimp using a cocktail of bioprotective lactic acid bacteria. | tropical shrimp is of considerable economic importance in the world but is highly perishable due to microbial and chemical degradation. biopreservation is a food preservation technology based on the addition of "positive" bacteria able to kill or prevent the growth of undesirable microorganisms. two strains of lactic acid bacteria (lab) have previously been selected for a biopreservation strategy: lactococcus piscium cncm i-4031, for its ability to prevent the sensory deterioration of seafood an ... | 2017 | 27760400 |
| maltaricin cpn, a new class iia bacteriocin produced by carnobacterium maltaromaticum cpn isolated from mould-ripened cheese. | the purpose of this study was to isolate, characterize and determine the structure and the antibacterial activities of a bacteriocin produced by carnobacterium maltaromaticum cpn, a strain isolated from unpasteurized milk camembert cheese. | 2016 | 27489131 |
| isolation and taxonomic identity of bacteriocin-producing lactic acid bacteria from retail foods and animal sources. | bacteriocin-producing (bac⁺) lactic acid bacteria (lab) were isolated from a variety of food products and animal sources. samples were enriched in de man, rogosa, and sharpe (mrs) lactocilli broth and plated onto mrs agar plates using a "sandwich overlay" technique. inhibitory activity was detected by the "deferred antagonism" indicator overlay method using listeria monocytogenes as the primary indicator organism. antimicrobial activity against l. monocytogenes was detected by 41 isolates obtain ... | 2015 | 27682080 |
| stress response and adaptation of listeria monocytogenes 08-5923 exposed to a sublethal dose of carnocyclin a. | carnocyclin a (ccla) is an antimicrobial peptide produced by carnobacterium maltaromaticum atcc pta-5313, which can be used to control the growth of listeria monocytogenes in ready-to-eat meat products. the aim of this research was to elucidate the cellular responses of l. monocytogenes 08-5923 exposed to a sublethal dose of ccla. microarray, quantitative reverse transcription-pcr, tandem mass spectrometry, and electron microscopy were used to investigate the alteration in gene expression, prote ... | 2014 | 24747893 |
| purification and characterization of antimicrobial peptides from fish isolate carnobacterium maltaromaticum c2: carnobacteriocin x and carnolysins a1 and a2. | carnobacterium maltaromaticum c2, isolated from brazilian smoked fish (surubim, pseudoplatystoma sp.), was found to exert antimicrobial activity against listeria monocytogenes, an important foodborne pathogen. in this study, the bacteriocins produced by c. maltaromaticum c2 were purified via an extraction with xad-16 resin, a c18 solid phase extraction, followed by reversed-phase fast protein liquid chromatography. the purified active fractions were characterized using tandem mass spectrometry, ... | 2014 | 24412962 |
| characterization of carnobacterium maltaromaticum lma 28 for its positive technological role in soft cheese making. | carnobacterium maltaromaticum is a lactic acid bacterium isolated from soft cheese. the objective of this work was to study its potential positive impact when used in cheese technology. phenotypic and genotypic characterization of six strains of c. maltaromaticum showed that they belong to different phylogenetic groups. although these strains lacked the ability to coagulate milk quickly, they were acidotolerant. they did not affect the coagulation capacity of starter lactic acid bacteria, lactoc ... | 2013 | 24010601 |
| cell-free supernatants obtained from fermentation of cheese whey hydrolyzates and phenylpyruvic acid by lactobacillus plantarum as a source of antimicrobial compounds, bacteriocins, and natural aromas. | cheese whey hydrolyzates supplemented with phenylpyruvic acid (ppa) and commercial nutrients can be efficiently metabolized by lactobacillus plantarum cect-221 to biosynthesize some compounds with attractive applications in the food market. the main metabolites of cell-free extracts were antimicrobial compounds such as phenyllactic acid (pla) and lactic acid (la). the production of pla by l. plantarum cect-221 was evaluated in the man-rogosa-sharpe broth supplemented with two biosynthetic precur ... | 2013 | 23934083 |
| tuberculosis and the risk of infection with other intracellular bacteria: a population-based study. | summary persons who develop tuberculosis (tb) may have subtle immune defects that could predispose to other intracellular bacterial infections (icbis). we obtained data on tb and five icbis (chlamydia trachomatis, salmonella spp., shigella spp., yersinia spp., listeria monocytogenes) reported to the tennessee department of health, usa, 2000-2011. incidence rate ratios (irrs) comparing icbis in persons who developed tb and icbis in the tennessee population, adjusted for age, sex, race and ethnici ... | 2015 | 25148655 |
| eukaryotic initiation factor 2 (eif2) signaling regulates proinflammatory cytokine expression and bacterial invasion. | in eukaryotic cells, there are two well characterized pathways that regulate translation initiation in response to stress, and each have been shown to be targeted by various viruses. we recently showed in a yeast-based model that the bacterial virulence factor yopj disrupts one of these pathways, which is centered on the α-subunit of the translation factor eif2. here, we show in mammalian cells that induction of the eif2 signaling pathway occurs following infection with bacterial pathogens and t ... | 2012 | 22761422 |
| activation of type iii interferon genes by pathogenic bacteria in infected epithelial cells and mouse placenta. | bacterial infections trigger the expression of type i and ii interferon genes but little is known about their effect on type iii interferon (ifn-λ) genes, whose products play important roles in epithelial innate immunity against viruses. here, we studied the expression of ifn-λ genes in cultured human epithelial cells infected with different pathogenic bacteria and in the mouse placenta infected with listeria monocytogenes. we first showed that in intestinal lovo cells, induction of ifn-λ genes ... | 2012 | 22720036 |
| contribution of selective conditions to microbial competition in four listeria selective enrichment formulations. | microbial competition during selective enrichment negatively affects listeria monocytogenes populations and may hinder the subsequent detection or recovery of this organism. competition assays among 10 selected strains of listeria and citrobacter braakii were performed in buffered listeria enrichment broth, 3-(n-morpholino)propanesulfonic acid-buffered listeria enrichment broth, university of vermont medium-modified listeria enrichment broth, and fraser broth. the individual contributions of eac ... | 2016 | 28221922 |
| experimental application of lactobacillus fermentum ccm 7421 in combination with chlorophyllin in dogs. | chlorophyll belongs in a larger class of phytochemical plant pigments currently receiving more attention as a physiologically active dietary component. although most research has focused on its biological activities such as its antioxidant, antimutagenic, anti-inflammatory or apoptotic effects in humans or rodents, there is limited knowledge at this time about the combinative possibilities of chlorophyll with probiotic bacteria. our aim was to test the growth characteristics of canine-derived pr ... | 2015 | 26062536 |
| characterization of a bacteriocin produced by lactococcus lactis subsp. lactis crl 1584 isolated from a lithobates catesbeianus hatchery. | lactococcus lactis crl 1584 isolated from a lithobates catesbeianus hatchery inhibits the growth of citrobacter freundii (a bullfrog pathogen) and listeria monocytogenes by a synergistic effect between lactic acid, hydrogen peroxide and a bacteriocin-like molecule. the chemical characterization of the bacteriocin in cell-free supernatants indicates that it has a proteinaceous nature. hexadecane and ethyl acetate did not modify the bacteriocin activity, while 10 and 20 % (v/v) chloroform decrease ... | 2014 | 24150985 |
| microbiological quality of ready-to-eat salads: an underestimated vehicle of bacteria and clinically relevant antibiotic resistance genes. | the increase demand for fresh vegetables is causing an expansion of the market for minimally processed vegetables along with new recognized food safety problems. to gain further insight on this topic we analyzed the microbiological quality of portuguese ready-to-eat salads (rts) and their role in the spread of bacteria carrying acquired antibiotic resistance genes, food products scarcely considered in surveillance studies. a total of 50 rts (7 brands; split or mixed leaves, carrot, corn) were co ... | 2013 | 24036261 |
| phenotypic complementation of genetic immunodeficiency by chronic herpesvirus infection. | variation in the presentation of hereditary immunodeficiencies may be explained by genetic or environmental factors. patients with mutations in hoil1 (rbck1) present with amylopectinosis-associated myopathy with or without hyper-inflammation and immunodeficiency. we report that barrier-raised hoil-1-deficient mice exhibit amylopectin-like deposits in the myocardium but show minimal signs of hyper-inflammation. however, they show immunodeficiency upon acute infection with listeria monocytogenes, ... | 2015 | 25599590 |
| invasive listeriosis in a patient with several episodes of antibiotic associated colitis presumably due to clostridium difficile. | a 62-year-old man developed a blood stream infection and meningitis due to listeria monocytogenes, 20 days after an episode of pseudo-membranous colitis. the patient, hospitalized for the first time for transurethral prostatectomy, was readmitted 20 days later with watery diarrhea. pseudo-membranous colitis was diagnosed and treated successfully, without testing for clostridium difficile infection (cdi). after 15 more days, the patient developed again diarrhea, fever and confusion. hospitalized ... | 2017 | 28365900 |
| formicin - a novel broad-spectrum two-component lantibiotic produced by bacillus paralicheniformis apc 1576. | bacteriocins represent a rather underutilized class of antimicrobials despite often displaying activity against many drug-resistant pathogens. lantibiotics are a post-translationally modified class of bacteriocins, characterized by the presence of lanthionine and methyllanthionine bridges. in this study, a novel two-peptide lantibiotic was isolated and characterized. formicin was isolated from bacillus paralicheniformis apc 1576, an antimicrobial-producing strain originally isolated from the int ... | 2016 | 27450592 |
| an experimental platform using human intestinal epithelial cell lines to differentiate between hazardous and non-hazardous proteins. | human intestinal epithelial cell lines (t84, caco-2, and hct-8) grown on permeable transwell™ filters serve as models of the gastrointestinal barrier. in this study, this in vitro model system was evaluated for effectiveness at distinguishing between hazardous and non-hazardous proteins. indicators of cytotoxicity (ldh release, mtt conversion), monolayer barrier integrity ([(3)h]-inulin flux, horseradish peroxidase flux, trans-epithelial electrical resistance [teer]), and inflammation (il-8, il- ... | 2016 | 27060235 |
| the sactibiotic subclass of bacteriocins: an update. | the sactibiotics are a recently designated subclass of bacteriocins that contain characteristic cysteine sulphur to α -carbon linkages mediated through post-translational modifications. they are a relatively small subclass of bacteriocins compared to the most thoroughly studied lantibiotics. the sactibiotics that have been extensively studied thus far are thuricin cd, subtilosin a, thurincin h, and propionicin f. despite their recent discovery, there have already been significant advances made i ... | 2015 | 26031307 |
| rna-based fluorescent biosensors for live cell imaging of second messenger cyclic di-amp. | cyclic di-amp (cdia) is a second messenger predicted to be widespread in gram-positive bacteria, some gram-negative bacteria, and archaea. in the human pathogen listeria monocytogenes, cdia is an essential molecule that regulates metabolic function and cell wall homeostasis, and decreased levels of cdia result in increased antibiotic susceptibility. we have generated fluorescent biosensors for cdia through fusion of the spinach2 aptamer to ligand-binding domains of cdia riboswitches. the biosens ... | 2015 | 25965978 |
| improved detection of gastrointestinal pathogens using generalised sample processing and amplification panels. | we aimed to streamline the diagnosis of gastrointestinal disease by producing multiplexed real time polymerase chain reaction (pcr) panels employing universal sample processing for dna and rna containing pathogens. a total of 487 stored, previously characterised stool samples comprising bacterial, viral, protozoan and clostridium difficile positive samples were tested using four multiplexed real time pcr panels. a further 81 pre-selected clinical samples from a teaching hospital were included to ... | 2014 | 24300711 |
| emerging infectious colitis. | the present review will highlight recent advances in the knowledge of emerging pathogens causing infectious colitis and provide a description of the most important food-borne outbreaks. | 2014 | 24275672 |
| management of microbial food safety in arab countries. | microbial food safety remains a major economic and public health concern in arab countries. over the past several years, many of these countries have attempted to revise and upgrade food quality control and surveillance programs; however, these systems vary in scope and effectiveness. this review addresses the major reported foodborne outbreaks and multidrug resistance of pathogenic microorganisms isolated from food products. major foodborne pathogens of concern included brucella spp., clostridi ... | 2012 | 23127718 |
| production of bacteriocin by leuconostoc mesenteroides 406 isolated from mongolian fermented mare's milk, airag. | the purification and characterization of a bacteriocin produced by leuconostoc mesenteroides strain 406 that was isolated from traditional mongolian fermented mare's milk, airag, were carried out. leuconostoc mesenteroides strain 406 was identified on the basis of its morphological and biochemical characteristics and carbohydrate fermentation profile and by api 50 ch kit and 16s ribosomal dna analyses. the neutral-ph cell-free supernatant of this bacterium inhibited the growth of several lactic ... | 2012 | 23035710 |
| galleria mellonella as an in vivo model for assessing the protective activity of probiotics against gastrointestinal bacterial pathogens. | the antagonistic activity against gastrointestinal bacterial pathogens is an important property of probiotic bacteria and a desirable feature for pre-selection of novel strains with probiotic potential. pre-screening of candidate probiotics for antibacterial activity should be based on in vitro and in vivo tests. this study investigated whether the protective activity of probiotic bacteria against gastrointestinal bacterial pathogens can be evaluated using galleria mellonella larvae as an in viv ... | 2017 | 28369512 |
| seeds of the wild progenitor of maize possess bacteria that antagonize foodborne pathogens. | endophytes are microorganisms that inhabit plant tissues without causing disease. some endophytes help their hosts to combat pathogens. here we explored the hypothesis that the plant-derived foods consumed by humans and other animals host endophytes that also antagonize foodborne pathogens or food-rotting agents. our laboratory previously cultured a library of bacterial endophytes from different members of the maize/corn family (zea) including wild relatives. here, 190 of these endophytes were s ... | 2017 | 28186422 |
| the microbiota of marketed processed edible insects as revealed by high-throughput sequencing. | entomophagy has been linked to nutritional, economic, social and ecological benefits. however, scientific studies on the potential safety risks in eating edible insects need to be carried out for legislators, markets and consumers. in this context, the microbiota of edible insects deserves to be deeply investigated. the aim of this study was to elucidate the microbial species occurring in some processed marketed edible insects, namely powdered small crickets, whole dried small crickets (acheta d ... | 2017 | 27889142 |
| enterocin b3a-b3b produced by lab collected from infant faeces: potential utilization in the food industry for listeria monocytogenes biofilm management. | enterococcus faecalis b3a-b3b produces the bacteriocin b3a-b3b with activity against listeria monocytogenes, staphylococcus aureus, methicillin-resistant staphylococcus aureus (mrsa) and clostridium perfringens, but apparently not against fungi or gram-negative bacteria, except for salmonella newport. b3a-b3b enterocin has two different nucleotides but similar amino acid composition to the class iib mr10a-mr10b enterocin. b3a-b3b consists of two peptides of predicted molecular mass of 5176.31 da ... | 2017 | 27878401 |
| in vitro probiotic characteristics of lactobacillus plantarum zdy 2013 and its modulatory effect on gut microbiota of mice. | lactobacillus plantarum zdy 2013, a novel strain isolated from chinese traditional fermented acid beans, was systematically evaluated for its survival capacity under stress conditions (ph, bile salt, simulated gastrointestinal tract, and antibiotics), production of exopolysaccharide and antagonism against 8 pathogens. its effect on mice gut microbiota was also investigated by quantitative pcr and pcr-denaturing gradient gel electrophoresis. the results showed that zdy 2013 can grow at ph 3.5 and ... | 2015 | 26142853 |
| an assessment of the human health impact of seven leading foodborne pathogens in the united states using disability adjusted life years. | we explored the overall impact of foodborne disease caused by seven leading foodborne pathogens in the united states using the disability adjusted life year (daly). we defined health states for each pathogen (acute illness and sequelae) and estimated the average annual incidence of each health state using data from public health surveillance and previously published estimates from studies in the united states, canada and europe. these pathogens caused about 112 000 dalys annually due to foodborn ... | 2015 | 25633631 |
| proportion of illness acquired by foodborne transmission for nine enteric pathogens in australia: an expert elicitation. | estimates of the burden of illness acquired from food inform public health policy and prioritize interventions. a key component of such estimates is the proportion of illnesses that are acquired by foodborne transmission. in view of the shortage of requisite data, these proportions are commonly obtained through a process known as expert elicitation. we report findings from an elicitation process used to assess the importance of the foodborne transmission route for nine pathogens in australia, ci ... | 2014 | 25072416 |
| microbiological and chemical evaluation of helix spp. snails from local and non-eu markets, utilised as food in sardinia. | with this study, 28 pools of snails of the genus helix, respectively helix aspersa (n=24) and helix vermiculata (n=4) were analysed. they were taken from snail farming and stores. the snails were from sardinia, other regions of italy, and from abroad. all the samples were examined as pool looking for these microbiological target: salmonella spp., listeria monocytogenes, escherichia coli o157, clostridium perfringens, norovirus and hepatitis a virus (hav). in the same pools, the concentration of ... | 2014 | 27800346 |
| disease-outcome trees, eq-5d scores, and estimated annual losses of quality-adjusted life years (qalys) for 14 foodborne pathogens in the united states. | measures of disease burden such as quality-adjusted life years (qalys) are increasingly important to risk-based food safety policy. they provide a means of comparing relative risk from diverse health outcomes. we present detailed disease-outcome trees and eq-5d scoring for 14 major foodborne pathogens representing over 95% of foodborne illnesses, hospitalizations, and deaths due to specified agents in the united states (campylobacter spp., clostridium perfringens, cryptosporidium parvum, cyclosp ... | 2014 | 24588853 |
| screening of bacteriocin-producing enterococcus faecalis strains for antagonistic activities against clostridium perfringens. | this study was conducted to isolate and characterize bacteriocin-producing bacteria against clostridium perfringens (c. perfringens) from domestic animals to determine their usefulness as probiotics. bacteriocin-producing bacteria were isolated from pig feces by the spot-on-lawn method. a total of 1,370 bacterial stains were isolated, and six were tentatively selected after identifying the inhibitory activity against the pathogenic indicator c. perfringens kctc 3269 and kctc 5100. the selected s ... | 2014 | 26761495 |
| technological properties of bacteriocin-producing lactic acid bacteria isolated from pico cheese an artisanal cow's milk cheese. | evaluate technologically relevant properties from bacteriocin-producing strains to use as starter/adjunct cultures in cheese making. | 2014 | 24206097 |
| bio-preservation of ground beef meat by enterococcus faecalis cect7121. | meat and particularly ground beef is frequently associated with food poisoning episodes and breeches in food safety. the main goal of this research was to evaluate the bactericide effect of the probiotic enterococcus faecalis cect7121, against different pathogens as: escherichia coli o157:h7, staphylococcus aureus, clostridium perfringens and listeria monocytogenes, inoculated in ground beef meat. three studies were performed to evaluate the inhibition of e. faecalis cect7121 on ground beef meat ... | 2013 | 24159282 |