| inhibitory effect of cinnamon powder on pathogen growth in laboratory media and oriental-style rice cakes (sulgidduk). | there has been an increasing interest in the use of natural plant materials as alternative food preservatives. we examined the antimicrobial effects of natural plant materials used as additives against foodborne pathogens in laboratory media and sulgidduk, oriental-style rice cakes. cinnamon, mugwort, and garlic powder solutions (3%) were tested for their antimicrobial activities against pathogens in laboratory media. sulgidduk prepared with different amounts of cinnamon powder (1, 3, and 6%) wa ... | 2013 | 23317869 |
| inactivation of foodborne pathogens in ground beef by cooking with highly controlled radio frequency energy. | the consumer demand for fresh tasting, high quality, low salt, preservative-free meals which require minimal preparation time magnifies the safety concern and emphasizes the need to use innovative technologies for food processing. a modern technique to uniformly heat and cook foods is based on a combination of convection and controlled radio frequency (rf) energy. however any advantage conferred on meat cooked by this method would be lost if application of the technology results in decreased saf ... | 2013 | 23290228 |
| assessment of the microbial safety and quality of cooked chilled foods and their production process. | refrigerated processed foods of extended durability (repfeds) are a heterogeneous group of food products. this study assesses the microbial safety and quality along the production process in five repfed companies. samples were taken of raw materials (n=123), intermediate products (n=123), end products at production day (n=45) and at end of shelf life (n=90), food contact surfaces (n=226) and worker's hands/gloves (n=92). samples are analysed for total psychrotrophic aerobic count, aerobic spore ... | 2013 | 23290224 |
| changes in microbial contamination levels of porcine carcasses and fresh pork in slaughterhouses, processing lines, retail outlets, and local markets by commercial distribution. | the objective of this study was to evaluate the changes in microbial contamination levels of each porcine carcass and fresh pork in a general distribution process. a total of 100 commercial pigs were sampled (six sampling sites per individual, total 600 samples) at four sequential stages: slaughterhouse (after carcass grading and boning), processing line, retail outlet, and local market. no significant differences were observed in the contaminant percentages among sampling sites and sample colle ... | 2013 | 23273786 |
| production of medium chain saturated fatty acids with enhanced antimicrobial activity from crude coconut fat by solid state cultivation of yarrowia lipolytica. | fatty acids profiles and antimicrobial activity of crude coconut fat hydrolysates obtained in solid-state cultivation system with a selected yeast strain yarrowia lipolytica ro13 were performed. a preliminary step regarding extracellular lipase production and solid state enzymatic hydrolysis of crude fat at different water activity and time intervals up to 7 days was also applied. gas chromatography-mass spectrometry analysis was used for quantification of medium chain saturated fatty acids (mcs ... | 2013 | 23194533 |
| probiotic potential of lactobacillus spp. isolated from brazilian regional ovine cheese. | twelve lactobacillus isolates from brazilian starter-free ovine cheeses were evaluated for their probiotic potential. the strains were identified by 16s rdna sequencing as lactobacillus plantarum (7), lb. brevis (2), lb. casei (2) and lb. parabuchneri (1). all strains showed variable resistance to gastric juices and relative tolerance to pancreatin and bile salts. only five strains of lb. plantarum could not deconjugate the sodium salt of taurodeoxycholic acid. autoaggregation ability after 24 h ... | 2012 | 23171587 |
| chemical composition and antioxidative activity of echinophora platyloba dc. essential oil, and its interaction with natural antimicrobials against food-borne pathogens and spoilage organisms. | this study was undertaken to determine the chemical composition and antioxidative capacity of echinophora platyloba dc. essential oil, and its antimicrobial potency against listeria monocytogenes, bacillus cereus, bacillus subtilis, staphylococcus aureus, salmonella typhimurium, escherichia coli o157:h7, pseudomonas aeruginosa, candida albicans, candida tropicalis, rhodotorula rubra, and rhodotorula mucilaginosa. the essential oil was analyzed by gc and gc-ms; and evaluated for its antioxidative ... | 2012 | 23106123 |
| changes in microbial contamination levels and prevalence of foodborne pathogens in alfalfa (medicago sativa) and rapeseed (brassica napus) during sprout production in manufacturing plants. | samples were taken from three sprout processing plants at five different stages of production (a total of 20 investigations). quantitative analyses comprised aerobic plate counts (apcs) and the measurement of coliforms and bacillus cereus levels, whereas qualitative analyses involved assessing the levels of escherichia coli and major foodborne pathogens (e. coli o157:h7, listeria monocytogenes, salmonella spp., and staphylococcus aureus). the apc for alfalfa seeds (3·71-4·61 log cfu g(-1)) and r ... | 2013 | 23051104 |
| technological properties and probiotic potential of enterococcus faecium strains isolated from cow milk. | to identify enterococci from the fermentation of milk for the production of nono, an african fermented dairy product, to determine the technological properties for suitability as starter cultures and safety as probiotics. | 2013 | 23035976 |
| characterization of lactic acid bacteria from naturally-fermented manzanilla aloreña green table olives. | manzanilla aloreña (or aloreña) table olives are naturally fermented traditional green olives with a denomination of protection (dop). the aim of this study was to search for lactic acid bacteria (lab) with technological properties of interest for possible inclusion in a starter or protective culture preparation or also as probiotics. a collection of 144 lab obtained from aloreña green table olives naturally-fermented by four small-medium enterprises (smes) from málaga (spain), including lactoba ... | 2012 | 22986194 |
| comparison of sample preparation methods for the recovery of foodborne pathogens from fresh produce. | sample preparation methods (pummeling, pulsifying, sonication, and shaking by hand) were compared for achieving maximum recovery of foodborne pathogens from iceberg lettuce, perilla leaves, cucumber, green pepper, and cherry tomato. antimicrobial and dehydration effects also were examined to investigate causes of poor recovery of pathogens. each produce type was inoculated with escherichia coli o157:h7, salmonella typhimurium, listeria monocytogenes, staphylococcus aureus, and bacillus cereus at ... | 2012 | 22980003 |
| functional properties of some new zealand fruit extracts towards selected probiotic and pathogenic bacteria. | the functional properties of aqueous extracts of four fruits commonly consumed in new zealand, namely blueberry, strawberry, green kiwifruit and feijoa, towards the growth of probiotic and pathogenic bacteria were investigated in this study. a concentration range of 0.1-30.0 g/l was evaluated in a high-throughput assay to determine their effects. in general, the four fruit extracts exerted growth-stimulating effects on the probiotic bacteria, except with bifidobacterium longum, whereas they were ... | 2012 | 22968373 |
| detection and enumeration of four foodborne pathogens in raw commingled silo milk in the united states. | a nationwide survey was conducted to obtain qualitative and quantitative data on bacterial contamination of raw commingled silo milk intended for pasteurization. the levels of total aerobic bacteria, total coliforms, enterobacteriaceae, escherichia coli, and staphylococcus aureus were determined using the tempo system. the prevalence rates and levels of presumptive bacillus cereus, e. coli o157:h7, listeria monocytogenes, and salmonella spp. were determined in 214 samples. b. cereus was detected ... | 2012 | 22856561 |
| identification of a new bacillus licheniformis strain producing a bacteriocin-like substance. | the emergence of antibiotic resistance has spurred a great number of studies for development of new antimicrobials in the past decade. the purpose of this study was to screen environmental samples for bacillus strains producing potent antimicrobial agents. a new strain, which showed strong antimicrobial activity against staphylococcus aureus and salmonella enterica ser. pullorum, was isolated from soil and designated as b116. this new isolate was identified as bacillus licheniformis by morpholog ... | 2012 | 22752909 |
| properties of bac w42, a bacteriocin produced by bacillus subtilis w42 isolated from cheonggukjang. | ten bacillus strains with antimicrobial activities were isolated from cheonggukjang produced at different parts in korea. they all inhibited listeria monocytogenes atcc 19111 and nine inhibited bacillus cereus atcc 14579. four isolates (w42, h27, ske 12, and k21) showing strong inhibiting activities were identified as b. subtilis. b. subtilis w42 was the most inhibiting strain. the antimicrobial activity of culture supernatant from b. subtilis w42 was destroyed completely by proteinase k treatme ... | 2012 | 22713985 |
| fluorescence-based comparative evaluation of bactericidal potency and food application potential of anti-listerial bacteriocin produced by lactic acid bacteria isolated from indigenous samples. | the aim of the present study was to ascertain the potency of anti-listerial bacteriocin produced by lactic acid bacteria (lab) isolated from indigenous samples of dahi, dried fish, and salt-fermented cucumber. a total of 231 lab isolates were obtained from the samples, of which 51 isolates displayed anti-listerial activity. the anti-listerial lab were identified by pcr as lactobacillus sp., pediococcus sp., enterococcus sp., and lactococcus sp. pcr also enabled the detection of class iia bacteri ... | 2012 | 26781854 |
| comparison of the potency of the lipid ii targeting antimicrobials nisin, lacticin 3147 and vancomycin against gram-positive bacteria. | while nisin (lantibiotic), lacticin 3147 (lantibiotic) and vancomycin (glycopeptides) are among the best studied lipid ii-binding antimicrobials, their relative activities have never been compared. nisin and lacticin 3147 have been employed/investigated primarily as food preservatives, although they do have potential in terms of veterinary and clinical applications. vancomycin is used exclusively in clinical therapy. we reveal a higher potency for lacticin 3147 (mic 0.95-3.8 μg/ml) and vancomyci ... | 2012 | 26781852 |
| novel polymer anchored cr(iii) schiff base complexes: synthesis, characterization and antimicrobial properties. | new polymer-bound schiff bases and cr(iii) complexes have been synthesized by the reaction of 4-benzyloxybenzaldehyde, polymer-bound with 2-aminophenol, 2-amino-4-chlorophenol and 2-amino-4-methylphenol. the structure of polymeric-schiff bases and their cr(iii) complexes have been characterized by elemental analyses, magnetic measurements, ir, uv-vis, tg-dta and (1)h-nmr. all these compounds have also been investigated for antibacterial activity by the well-diffusion method against staphylococcu ... | 2012 | 22622060 |
| development of sandwich enzyme-linked immunosorbent assay for the detection of cronobacter muytjensii (formerly called enterobacter sakazakii). | this study aimed to produce a polyclonal antibody against cronobacter muytjensii (c. muytjensii, formerly called enterobacter sakazakii) and to develop an immunoassay for its detection. the optimum production of rabbit anti-c. muytjensii immunoglobulin g (igg) and chicken anti-c. muytjensii igy was reached in weeks 8 and 9, respectively. purification of rabbit anti-c. muytjensii igg from immunized rabbit sera was accomplished using the caprylic acid and ammonium sulfate precipitation method. as ... | 2012 | 22519814 |
| microbiological performance of a food safety management system in a food service operation. | the microbiological performance of a food safety management system in a food service operation was measured using a microbiological assessment scheme as a vertical sampling plan throughout the production process, from raw materials to final product. the assessment scheme can give insight into the microbiological contamination and the variability of a production process and pinpoint bottlenecks in the food safety management system. three production processes were evaluated: a high-risk sandwich p ... | 2012 | 22488059 |
| characterization of lysb4, an endolysin from the bacillus cereus-infecting bacteriophage b4. | bacillus cereus is a foodborne pathogen that causes emetic or diarrheal types of food poisoning. the incidence of b. cereus food poisoning has been gradually increasing over the past few years, therefore, biocontrol agents effective against b. cereus need to be developed. endolysins are phage-encoded bacterial peptidoglycan hydrolases and have received considerable attention as promising antibacterial agents. | 2012 | 22416675 |
| volatile organic compounds from centaurium erythraea rafn (croatia) and the antimicrobial potential of its essential oil. | gc and ms were used for the analysis of croatian centaurium erythraea rafn essential oil (obtained by hydrodistillation) and headspace (applying headspace solid-phase microextraction). the headspace contained numerous monoterpene hydrocarbons (the major ones were terpinene-4-ol, methone, p-cymene, γ-terpinene and limonene). oxygenated monoterpenes were present in the headspace and oil, while 1,8-cineole, bornyl acetate and verbenone were present only in the headspace. high headspace percentages ... | 2012 | 22349896 |
| assessment of microbial quality of commercial and home-made tiger-nut beverages. | we aimed to assess the microbiological quality of spanish commercial tiger-nut beverages as well as home-made samples collected from supermarkets, street vendors, juice bars and ice-cream parlours located in valencia. | 2012 | 22283438 |
| antimicrobial activity of enterococcus faecium fair-e 198 against gram-positive pathogens. | this study investigated the antimicrobial activity of enterococcus faecium fair-e 198 against bacillus cereus, listeria monocytogenes and staphylococcus aureus. using the critical-dilution method, the bacteriocin produced by e. faecium fair-e 198 inhibited all l. monocytogenes strains evaluated (1,600 to 19,200 au ml(-1)). however, none of the b. cereus and s. aureus strains investigated were inhibited. the maximum activity of this bacteriocin (800 au ml(-1)) was observed in mrs broth, while the ... | 2010 | 24031466 |
| simultaneous detection of pathogenic b. cereus, s. aureus and l. monocytogenes by multiplex pcr. | three important foodborne pathogens, bacillus cereus, listeria monocytogenes and staphylococcus aureus are of major concern for food safety in terms of frequency and seriousness of the disease. the occurrence these three important pathogens and their coexistence in food matrices are predominant. moreover, symptoms associated with b. cereus and s. aureus food poisoning not only closely resembles each other but can also be overlapping with other foodborne infections. in this context, detection of ... | 2009 | 23100783 |
| antimicrobial activity of various cationic molecules on foodborne pathogens. | antibacterial effects of various arginine- and lysine-rich polycationic proteins and polymers were evaluated by broth and solid dilution assay on a range of foodborne pathogens, gram-positive and gram-negative bacteria. the minimum inhibitory concentration (mic) and the minimum bactericidal concentration (mbc) of α-poly-l-lysine (poly-lys), α-poly-l-arginine (poly-arg) and protamines from herring sperm (clupeine sulphate) and salmon sperm (salmine sulphate) were determined on bacillus subtilis, ... | 2007 | 27517822 |
| potential probiotic attributes of a new strain of bacillus coagulans cgmcc 9951 isolated from healthy piglet feces. | a new strain of bacillus coagulans cgmcc 9551, which has a broad range of antibacterial activities against six main pathogenic bacteria including escherichia coli o8, staphylococcus aureus, salmonella enterica subsp. enterica serovar enteritidis, streptococcus suis, listeria monocytogenes and pasteurella multocida, was isolated from healthy piglet feces. in adhesion assay, the isolate exhibited a stronger adhesion to pig intestinal mucus than that of b. subtilis jt143 and l. acidophilus ly24 res ... | 2015 | 25752235 |
| inhibition of listeria monocytogenes biofilms by bacteriocin-producing bacteria isolated from mushroom substrate. | this study was designed to investigate the ability of naturally occurring bacteria isolated from mushroom substrate to prevent biofilm formation by listeria monocytogenes or to remove existing biofilms in mushroom production facilities. | 2017 | 27797439 |
| bacillus licheniformis contains two more perr-like proteins in addition to perr, fur, and zur orthologues. | the ferric uptake regulator (fur) family proteins include sensors of fe (fur), zn (zur), and peroxide (perr). among fur family proteins, fur and zur are ubiquitous in most prokaryotic organisms, whereas perr exists mainly in gram positive bacteria as a functional homologue of oxyr. gram positive bacteria such as bacillus subtilis, listeria monocytogenes and staphylococcus aureus encode three fur family proteins: fur, zur, and perr. in this study, we identified five fur family proteins from b. li ... | 2016 | 27176811 |
| investigation of the antimicrobial activity of bacillus licheniformis strains isolated from retail powdered infant milk formulae. | this study investigated the potential antimicrobial activity of ten bacillus licheniformis strains isolated from retail infant milk formulae against a range of indicator (lactococcus lactis, lactobacillus bulgaricus and listeria innocua) and clinically relevant (listeria monocytogenes, staphylococcus aureus, streptococcus agalactiae, salmonella typhimurium and escherichia coli) microorganisms. deferred antagonism assays confirmed that all b. licheniformis isolates show antimicrobial activity aga ... | 2014 | 24676765 |
| novel antilisterial bacteriocin licheniocin 50.2 from bacillus licheniformis vps50.2 isolated from soil sample. | to isolate and characterize bacteriocin, licheniocin 50.2, from soil bacteria identified as bacillus licheniformis. | 2014 | 24238327 |
| evaluation of food protective property of five natural products using fresh-cut apple slice model. | the present study evaluated the antioxidant (aa), antimicrobial and preservation effects of five plant derived natural products viz., rosmarinic acid (ra), p-coumaric acid (pca), trans-cinnamic acid (tca), hydroxyphenyllactic acid (hpa) and caffeic acid (ca) along with synthetic compounds (ascorbic acid, gallic acid, citric acid and bha) on fresh cut apple slices. antimicrobial efficacy of these compounds against bacillus licheniformis, pseudomonas vulgaris, shigella boydii, salmonella typhi, st ... | 2012 | 22530437 |
| comparative evaluation of the antimicrobial activity of 19 essential oils. | in our research on natural compounds efficient against human pathogen or opportunist microorganisms contracted by food or water, the antimicrobial activity of 19 essential oils (eos) was investigated against 11 bacterial species (6 gram positive, 5 gram negative) and 7 fungal species (2 dermatophytes, 1 mould, 4 yeasts) using microdilution assays. five essential oils were obtained from tunisian plants (eotun): artemisia herba-alba asso, juniperus phoenicea l., rosmarinus officinalis l., ruta gra ... | 2016 | 26566647 |
| bioprocess development for the production of sonorensin by bacillus sonorensis mt93 and its application as a food preservative. | media composition and environmental conditions were optimized using statistical tools, plackett burman design and response surface methodology, to maximize the yield of a bacteriocin, named as sonorensin, from a new marine isolate bacillus sonorensis mt93 showing broad spectrum of antimicrobial activity. under optimized conditions, mt93 produced 15-fold higher yield of sonorensin compared to that under initial fermentation conditions. as oxygen supply is a critical parameter controlling growth a ... | 2015 | 25459843 |
| sonorensin: an antimicrobial peptide, belonging to the heterocycloanthracin subfamily of bacteriocins, from a new marine isolate, bacillus sonorensis mt93. | marine environments are the greatest fronts of biodiversity, representing a resource of unexploited or unknown microorganisms and new substances having potential applications. among microbial products, antimicrobial peptides (amps) have received great attention recently due to their applications as food preservatives and therapeutic agents. a new marine soil isolate producing an amp was identified as bacillus sonorensis based on 16s rrna gene sequence analysis. it produced an amp that showed a b ... | 2014 | 24610839 |
| an in vivo reporter assay for srna-directed gene control in gram-positive bacteria: identifying a novel srna target in staphylococcus aureus. | bacterial small regulatory rnas (srnas) play a major role in the regulation of various cellular functions. most srnas interact with mrna targets via an antisense mechanism, modifying their translation and/or degradation. despite considerable progresses in discovering srnas in gram-positive bacteria, their functions, for the most part, are unknown. this is mainly due to difficulties in identifying their targets. to aid in the identification of srna targets in gram-positive bacteria, we set up an ... | 2017 | 28369640 |
| biocontrol of the internalization of salmonella enterica and enterohaemorrhagic escherichia coli in mung bean sprouts with an endophytic bacillus subtilis. | internalization of salmonella enterica and enterohaemorrhagic escherichia coli (ehec) in seed sprouts poses a health risk to consumers, and the conventional sanitization methods are not always effective to reduce this risk. this study initiated a biocontrol approach to limit the internalization using endophytic bacillus subtilis strains, which were isolated from the inner tissue of mung bean seeds or lettuce stems. by using the deferred agar method, 12 strains of b. subtilis out of 94 putative b ... | 2017 | 28364624 |
| a redox-responsive transcription factor is critical for pathogenesis and aerobic growth of listeria monocytogenes. | bacterial pathogens have evolved sophisticated mechanisms to sense and adapt to redox stress in nature and within the host. however, deciphering the redox environment encountered by intracellular pathogens in the mammalian cytosol is challenging and remains poorly understood. in this study, we assessed the contributions of the two redox-responsive, spx-family transcriptional regulators to the virulence of listeria monocytogenes, a gram-positive facultative intracellular pathogen. spx-family prot ... | 2017 | 28193635 |
| antibacterial and antivirulence effect of 6-n-hydroxylaminopurine in listeria monocytogenes. | the emerging development of antibiotic resistant bacteria calls for novel types of antibacterial agents. in this work we examined the putative antibacterial effect of purine analogs in listeria monocytogenes we show that, among several tested purine analogs, only 6-n-hydroxylaminopurine (6-n-hap) reduces the viability of the gram-positive pathogen listeria monocytogenes as in bacillus subtilis, 6-n-hap terminates expression at guanine riboswitches in l. monocytogenes hence preventing expression ... | 2017 | 28062853 |
| subtilosin prevents biofilm formation by inhibiting bacterial quorum sensing. | subtilosin, the cyclic lantibiotic protein produced by bacillus subtilis katmira1933, targets the surface receptor and electrostatically binds to the bacterial cell membrane. in this study, subtilosin was purified using ammonium sulfate ((nh4)2so4) precipitation and purified via column chromatography. subtilosin's antibacterial minimum and sub-minimum inhibitory concentrations (mic and sub-mic) and anti-biofilm activity (biofilm prevention) were established. subtilosin was evaluated as a quorum ... | 2017 | 27914001 |
| purification and characterization of a novel anti-campylobacter bacteriocin produced by lactobacillus curvatus dn317. | the lactic acid bacteria (lab) microbiota of saudi chicken ceca was determined. from 60 samples, 204 isolates of lactic acid bacteria were obtained. three isolates produced antimicrobial activities against campylobacter jejuni, listeria monocytogenes, and bacillus subtilis. the isolate dn317, which had the highest activity against campylobacter jejuni atcc 33560, was identified as lactobacillus curvatus (genbank accession numbers: kx353849 and kx353850). full inhibitory activity was observed aft ... | 2016 | 27812926 |
| f-type bacteriocins of listeria monocytogenes: a new class of phage tail-like structures reveals broad parallel coevolution between tailed bacteriophages and high-molecular-weight bacteriocins. | listeria monocytogenes is a significant foodborne human pathogen that can cause severe disease in certain high-risk individuals. l. monocytogenes is known to produce high-molecular-weight, phage tail-like bacteriocins, or "monocins," upon induction of the sos system. in this work, we purified and characterized monocins and found them to be a new class of f-type bacteriocins. the l. monocytogenes monocin genetic locus was cloned and expressed in bacillus subtilis, producing specifically targeted ... | 2016 | 27457717 |
| subunit arrangement in gpsb, a regulator of cell wall biosynthesis. | gpsb, a key regulator of cell division in gram-positive bacteria, interacts with a key peptidoglycan synthase at the cell division septum, the penicillin binding protein pbp1 (a.k.a. pona). bacillus subtilis gpsb has been reported to interact with other components of the cell division machinery, including ezra, mrec, and prkc. in this study, we report an analysis of the arrangement of subunits in listeria monocytogenes gpsb by small-angle x-ray scattering. the resulting model has an elongated sh ... | 2016 | 27257764 |
| anti-biofilm and sporicidal activity of peptides based on wheat puroindoline and barley hordoindoline proteins. | the broad-spectrum activity of antimicrobial peptides (amps) and low probability of development of host resistance make them excellent candidates as novel bio-control agents. a number of amps are found to be cationic, and a small proportion of these are tryptophan-rich. the puroindolines (pin) are small, basic proteins found in wheat grains with proposed roles in biotic defence of seeds and seedlings. synthetic peptides based on their unique tryptophan-rich domain (trd) display antimicrobial pro ... | 2016 | 27238815 |
| arginine patch predicts the rna annealing activity of hfq from gram-negative and gram-positive bacteria. | the sm-protein hfq facilitates interactions between small non-coding rna (srna) and target mrnas. in enteric gram-negative bacteria, hfq is required for srna regulation, and hfq deletion results in stress intolerance and reduced virulence. by contrast, the role of hfq in gram-positive is less established and varies among species. the rna binding and rna annealing activity of hfq from escherichia coli, pseudomonas aeruginosa, listeria monocytogenes, bacillus subtilis, and staphylococcus aureus we ... | 2016 | 27049793 |
| screening of probiotic activities of lactobacilli strains isolated from traditional tibetan qula, a raw yak milk cheese. | in this study, 69 lactobacilli isolated from tibetan qula, a raw yak milk cheese, were screened for their potential use as probiotics. the isolates were tested in terms of: their ability to survive at ph 2.0, ph 3.0, and in the presence of 0.3% bile salts; tolerance of simulated gastric and intestinal juices; antimicrobial activity; sensitivity against 11 specific antibiotics; and their cell surface hydrophobicity. the results show that out of the 69 strains, 29 strains (42%) had survival rates ... | 2016 | 26954218 |
| the listeria monocytogenes fur-regulated virulence protein frva is an fe(ii) efflux p1b4 -type atpase. | listeria monocytogenes frva (lmo0641) is critical for virulence in the mouse model and is an ortholog of the bacillus subtilis fur- and perr-regulated fe(ii) efflux p1b4 -type atpase pfet. previously, frva was suggested to protect against heme toxicity. here, we demonstrate that an frva mutant is sensitive to iron intoxication, but not to other metals. expression of frva is induced by high iron and this induction requires fur. frva functions in vitro as a divalent cation specific atpase most str ... | 2016 | 26946370 |
| an advanced bioinformatics approach for analyzing rna-seq data reveals sigma h-dependent regulation of competence genes in listeria monocytogenes. | alternative σ factors are important transcriptional regulators in bacteria. while σ(b) has been shown to control a large regulon and play important roles in stress response and virulence in the pathogen listeria monocytogenes, the function of σ(h) has not yet been well defined in listeria, even though σ(h) controls a large regulon in the closely related non-pathogenic bacillus subtilis. | 2016 | 26880300 |
| purification and characteristics of a novel bacteriocin produced by enterococcus faecalis l11 isolated from chinese traditional fermented cucumber. | to purify and characterize a novel bacteriocin with broad inhibitory spectrum produced by an isolate of enterococcus faecalis from chinese fermented cucumber. | 2016 | 26875092 |
| identification and characterization of holgh15: the holin of staphylococcus aureus bacteriophage gh15. | holins are phage-encoded hydrophobic membrane proteins that spontaneously and non-specifically accumulate and form lesions in the cytoplasmic membrane. the orf72 gene (also designated holgh15) derived from the genome of the staphylococcus aureus phage gh15 was predicted to encode a membrane protein. an analysis indicated that the protein encoded by holgh15 potentially consisted of two hydrophobic transmembrane helices. this protein exhibited the structural characteristics of class ii holins and ... | 2016 | 26873847 |
| comparison of antimicrobial activities of newly obtained low molecular weight scorpion chitosan and medium molecular weight commercial chitosan. | in this study the antimicrobial activity of low molecular weight (3.22 kda) chitosan, obtained for the first time from a species belonging to the scorpiones, was screened against nine pathogenic microorganisms (seven bacteria and two yeasts) and compared with that of medium molecular weight commercial chitosan (mmwcc). it was observed that the antimicrobial activity of the low molecular weight scorpion chitosan (lmwsc) was specific to bacterial species in general rather than gram-negative or gra ... | 2016 | 26702952 |
| to modulate survival under secondary stress conditions, listeria monocytogenes 10403s employs rsbx to downregulate σb activity in the poststress recovery stage or stationary phase. | listeria monocytogenes is a saprophytic bacterium that thrives in diverse environments and causes listeriosis via ingestion of contaminated food. rsbx, a putative sigma b (σ(b)) regulator, is thought to maintain the ready state in the absence of stress and reset the bacterium to the initial state in the poststress stage in bacillus subtilis. we wondered whether rsbx is functional in l. monocytogenes under different stress scenarios. genetic deletion and complementation of the rsbx gene were comb ... | 2015 | 26637594 |
| secondary metabolites constituents and antioxidant, anticancer and antibacterial activities of etlingera elatior (jack) r.m.sm grown in different locations of malaysia. | etlingera elatior is a well-known herb in malaysia with various pharmaceutical properties. | 2015 | 26399961 |
| identification of conserved and species-specific functions of the listeria monocytogenes prsa2 secretion chaperone. | the gram-positive bacterium listeria monocytogenes is a facultative intracellular pathogen that relies on the regulated secretion and activity of a variety of proteins that sustain life within diverse environments. prsa2 has recently been identified as a secreted peptidyl-prolyl cis/trans isomerase and chaperone that is dispensable for bacterial growth in broth culture but essential for l. monocytogenes virulence. following host infection, prsa2 contributes to the proper folding and activity of ... | 2015 | 26216425 |
| label-free identification of individual bacteria using fourier transform light scattering. | rapid identification of bacterial species is crucial in medicine and food hygiene. in order to achieve rapid and label-free identification of bacterial species at the single bacterium level, we propose and experimentally demonstrate an optical method based on fourier transform light scattering (ftls) measurements and statistical classification. for individual rod-shaped bacteria belonging to four bacterial species (listeria monocytogenes, escherichia coli, lactobacillus casei, and bacillus subti ... | 2015 | 26193558 |
| employing carbon dots modified with vancomycin for assaying gram-positive bacteria like staphylococcus aureus. | by employing attractive performance of fluorescent carbon dots, we herein successfully established an assay for analyzing bacteria firstly. specifically, carbon dots with blue fluorescence were initially synthesized according to a previous report, and modified with vancomycin on their surfaces. subsequently, the prepared carbon dots were applied to detect staphylococcus aureus accompanied with a linear range of 3.18×10(5)-1.59×10(8) cfu/ml as well as a detection limit of 9.40×10(4) cfu/ml. compa ... | 2015 | 26188677 |
| biological activities and dna interactions of amanita ovoidea. | amanita ovoidea (bull.) link (amanitaceae) is a well-known species due to its pleasant aroma and flavor since ancient times in the worldwide. this species is also known in turkey and people consume it extensively. | 2015 | 25874387 |
| a jack of all trades: the multiple roles of the unique essential second messenger cyclic di-amp. | second messengers are key components of many signal transduction pathways. in addition to cyclic amp, ppgpp and cyclic di-gmp, many bacteria use also cyclic di-amp as a second messenger. this molecule is synthesized by distinct classes of diadenylate cyclases and degraded by phosphodiesterases. the control of the intracellular c-di-amp pool is very important since both a lack of this molecule and its accumulation can inhibit growth of the bacteria. in many firmicutes, c-di-amp is essential, maki ... | 2015 | 25869574 |
| bacillus subtilis subsp. subtilis cbmdc3f with antimicrobial activity against gram-positive foodborne pathogenic bacteria: uv-maldi-tof ms analysis of its bioactive compounds. | in this work a new bacillus sp. strain, isolated from honey, was characterized phylogenetically. its antibacterial activity against three relevant foodborne pathogenic bacteria was studied; the main bioactive metabolites were analyzed using ultraviolet matrix assisted laser desorption-ionization mass spectrometry (uv-maldi ms). bacillus cbmdc3f was phylogenetically characterized as bacillus subtilis subsp. subtilis after rrna analysis of the 16s subunit and the gyra gene (access codes genbank jx ... | 2015 | 25820813 |
| prospecting russula senecis: a delicacy among the tribes of west bengal. | russula senecis, a worldwide distributed mushroom, is exclusively popular among the tribal communities of west bengal for food purposes. the present study focuses on its reliable taxonomic identification through macro- and micro-morphological features, dna barcoding, confirmation of its systematic placement by phylogenetic analyses, myco-chemicals and functional activities. for the first time, the complete internal transcribed spacer region of r. senecis has been sequenced and its taxonomic posi ... | 2015 | 25780764 |
| metabolic gene expression shift by listeria monocytogenes in coculture biofilms. | coculture communities of microbes are more realistic and common in nature than in laboratory-grown pure cultures. in a mixed community, when resources with a potential role in growth are shared, conflict (as a consequence of competition) or cooperation is certain. in our study, this situation of conflict and cooperation was explored to understand the population dynamics and community behavior of listeria monocytogenes. the social behavioral response of l. monocytogenes to the presence of bacillu ... | 2015 | 25776109 |
| probiotic potential of lactobacillus strains isolated from sorghum-based traditional fermented food. | sorghum-based traditional fermented food was screened for potential probiotic lactic acid bacteria. the isolates were identified by biochemical, physiological and genetic methods. species identification was done by 16s rrna sequence analysis. the functional probiotic potential of the two lactobacillus species viz., lactobacillus plantarum (lact. plantarum) and lactobacillus pentosus (lact. pentosus) was assessed by different standard parameters. the strains were tolerant to ph 2 for 1 h and resi ... | 2015 | 25666113 |
| structural and biochemical analysis of the essential diadenylate cyclase cdaa from listeria monocytogenes. | the recently identified second messenger cyclic di-amp (c-di-amp) is involved in several important cellular processes, such as cell wall metabolism, maintenance of dna integrity, ion transport, transcription regulation, and allosteric regulation of enzyme function. interestingly, c-di-amp is essential for growth of the gram-positive model bacterium bacillus subtilis. although the genome of b. subtilis encodes three c-di-amp-producing diadenlyate cyclases that can functionally replace each other, ... | 2015 | 25605729 |
| characterization of bacteriocin like inhibitory substance produced by a new strain brevibacillus borstelensis ag1 isolated from 'marcha'. | in the present study, a bacterium isolated from marcha- a herbal cake used as traditional starter culture to ferment local wine in north east india, was evaluated for bacteriocin like inhibitory substance production and was tested against six food borne/spoilage causing pathogens viz. listeria monocytogenes mtcc 839, bacillus subtilis mtcc 121, clostridium perfringens mtcc 450, staphylococcus aureus, lactobacillus plantarum and leuconostoc mesenteroides mtcc 107 by using bit/disc method followed ... | 2014 | 25477937 |
| synthesis, antibacterial, antioxidant activity and qsar studies of novel 2-arylidenehydrazinyl-4-arylthiazole analogues. | a novel series of 2-arylidenehydrazinyl-4-arylthiazole analogues (3a-p) was designed and synthesized in excellent yields using a rapid, simple, efficient methodology. sixteen novel compounds were screened for in vitro antimicrobial activities against eleven bacteria, namely, staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, bacillus subtilis, klebsiella pneumonia, citrobacter freundii, cronobacter sakazakii, salmonella enteritidis, escherichia coli, yersinia pestis, and pseud ... | 2014 | 25450634 |
| production of antibacterial bombyx mori cecropin a in mealworm-pathogenic beauveria bassiana erl1170. | efforts are underway to produce antimicrobial peptides in yellow mealworms (tenebrio molitor), which can be developed as more effective and safer animal feed additives. in this work, we expressed bombyx mori (bm) cecropin-a in mealworms by the infection of transformed entomopathogenic beauveria bassiana erl1170. the active domain of bm cecropin a gene was tagged with a signal sequence of b. bassiana for extracellular secretion, and the fragment was inserted into erl1170 by the restriction enzyme ... | 2015 | 25429897 |
| bacillus spp. isolated from the conjunctiva and their potential antimicrobial activity against other eye pathogens. | in this study, we attempted to screen and investigate antibacterial activity of bacillus species, which were isolated from conjunctiva, against other eyes pathogens. | 2014 | 25320585 |
| antimicrobial properties of nisin after glycation with lactose, maltodextrin and dextran and the thyme oil emulsions prepared thereof. | to clarify the reported conflicting antimicrobial activities of nisin after glycation, nisin was glycated with lactose, maltodextrin, and dextran at 70 °c and 50% relative humidity for 1-24 h. nisin before and after glycation was studied for the first time to prepare thyme oil emulsions. the activity of glycated nisin and the thyme oil emulsions was tested in both tryptic soy broth (tsb) and 2% reduced fat milk. results showed that nisin glycated with a smaller saccharide for a longer duration h ... | 2014 | 25240139 |
| a function of diviva in listeria monocytogenes division site selection. | the cell division protein diviva influences protein transport via the accessory seca2 secretion route in listeria monocytogenes. in contrast, diviva from the closely related bacterium bacillus subtilis contributes to division site selection via the mincdj system. however, no classical min phenotype, i.e. filamentation and minicell production was observed with a listerial δdiviva mutant. this has prompted the speculation that division site selection is diviva-independent in l. monocytogenes. we a ... | 2014 | 25185533 |
| recognition of u-rich rna by hfq from the gram-positive pathogen listeria monocytogenes. | hfq is a post-transcriptional regulator that binds u- and a-rich regions of srnas and their target mrnas to stimulate their annealing in order to effect translation regulation and, often, to alter their stability. the functional importance of hfq and its rna-binding properties are relatively well understood in gram-negative bacteria, whereas less is known about the rna-binding properties of this riboregulator in gram-positive species. here, we describe the structure of hfq from the gram-positive ... | 2014 | 25150227 |
| rapid detection of listeria monocytogenes in raw milk and soft cheese by a redox potential measurement based method combined with real-time pcr. | the incidence of outbreaks of foodborne listeriosis has indicated the need for a reliable and rapid detection of the microbe in different foodstuffs. a method combining redox potential measurement and real-time polymerase chain reaction (pcr) was developed to detect listeria monocytogenes in artificially contaminated raw milk and soft cheese. food samples of 25 g or 25 ml were homogenised in 225 ml of listeria enrichment broth (leb) with oxford supplement, and the redox potential measurement tec ... | 2014 | 25038948 |
| in-vitro assessment of antioxidant and antimicrobial activities of methanol extracts and essential oil of thymus hirtus sp. algeriensis. | owing to the complexity of the antioxidant materials and their mechanism of actions, it is obvious that no single testing method is capable of providing a comprehensive picture of the antioxidant profile. the essential oil of the thymus specie may still possess other important activities in traditional medicine, it can be used in the treatment of fever and cough. this essential oil may also have an anticancer activity. | 2014 | 25022197 |
| qualitative detection of class iia bacteriocinogenic lactic acid bacteria from traditional chinese fermented food using a ygngv-motif-based assay. | in the present study, a ygngv-motif-based assay was developed and applied. given that there is an increasing demand for natural preservatives, we set out to obtain lactic acid bacteria (lab) that produce bacteriocins against gram-positive and gram-negative bacteria. we here isolated 123 lab strains from 5 types of traditional chinese fermented food and screened them for the production of bacteriocins using the agar well diffusion assay (awda). then, to acquire lab producing class iia bacteriocin ... | 2014 | 24681304 |
| in vitro evaluation of new functional properties of poly-γ-glutamic acid produced by bacillus subtilis d7. | we investigated the functionality of poly-γ-glutamic acid (γ-pga), which is produced by bacillus subtilis d7, for its potential applications in medicine and cosmetics. the γ-pga had angiotensin-converting enzyme (ace) inhibition activity. ace inhibition activity was dependent on the γ-pga concentration; the highest ace inhibition activity was observed at 1.25 mg/l of γ-pga. ic50 (0.108 mg/ml) of the γ-pga was lower than that of standard ace inhibitory drug, n-[(s)-mercapto-2-methylpropionyl]-l-p ... | 2014 | 24600308 |
| biological characteristics and probiotic effect of leuconostoc lactis strain isolated from the intestine of black porgy fish. | a strain of lactic acid bacteria, leuconostoc lactis, was isolated from the intestinal tract of black porgy, sparus macrocephalus, and identified by conventional biochemical characteristics and 16s rdna gene sequence analysis. the isolated strain had the ability of bile tolerance and resistance to low ph, and survived well in the trypsinase and pepsin solution. but the highly concentrated dose of trypsinase and pepsin affect the viability of the isolated strain. the isolate was resistant to seve ... | 2013 | 24516418 |
| intersection of the stringent response and the cody regulon in low gc gram-positive bacteria. | bacteria adapt efficiently to a wide range of nutritional environments. therefore, they possess overlapping regulatory systems that detect intracellular pools of key metabolites. in low gc gram-positive bacteria, two global regulators, the stringent response and the cody repressor, respond to an intracellular decrease in amino acid content. amino acid limitation leads to rapid synthesis of the alarmones pppgpp and ppgpp through the stringent response and inactivates the cody repressor. two cofac ... | 2014 | 24462007 |
| a new pentacyclic triterpene with potent antibacterial activity from limnophila indica linn. (druce). | a new pentacyclic triterpenoid constituent, characterized as 3-oxo-olean-12(13),18(19)-dien-29α-carboxylic acid (1) on the basis of detailed spectral studies, was isolated from the aerial parts and roots of limnophila indica (scrophulariaceae). compound 1 exhibited considerable antibacterial activity against three gram-positive bacteria viz. bacillus subtilis, staphylococcus aureus and listeria monocytogenes (mics within a range of 25-30 μg/ml) and moderate activity against four gram-negative ba ... | 2013 | 23876368 |
| fluoro-phenyl-styrene-sulfonamide, a novel inhibitor of σb activity, prevents the activation of σb by environmental and energy stresses in bacillus subtilis. | sigma b (σ(b)) is an alternative sigma factor that regulates the general stress response in bacillus subtilis and in many other gram-positive organisms. σ(b) activity in b. subtilis is tightly regulated via at least three distinct pathways within a complex signal transduction cascade in response to a variety of stresses, including environmental stress, energy stress, and growth at high or low temperatures. we probed the ability of fluoro-phenyl-styrene-sulfonamide (fpss), a small-molecule inhibi ... | 2013 | 23524614 |
| rsbv of listeria monocytogenes contributes to regulation of environmental stress and virulence. | sigmab factor is an important regulatory factor for stress response in gram-positive bacteria such as listeria monocytogenes (l. monocytogenes), staphylococcus aureus and bacillus subtilis. however, the activity of sigmab factor is regulated by rsbv factor. currently, the functional studies of rsbv factor are mostly focused on non-pathogenic b. subtilis, but the roles of rsbv factor in pathogenic l. monocytogenes during the regulation of environmental stress and virulence are still unclear. in t ... | 2013 | 23192174 |
| mannose receptor, c type 1 contributes to bacterial uptake by placental trophoblast giant cells. | during pregnancy, maternal immune function is strictly controlled and immune tolerance is induced. trophoblast giant (tg) cells exhibit phagocytic activity and show macrophage-like activity against microorganisms in the placenta. however, details of molecular receptors and mechanisms for uptake by tg cells have not been clarified. in this study, we investigated the involvement of the mannose receptor, c type 1 (mrc1), in the uptake of the abortion-inducible bacterium listeria monocytogenes and a ... | 2012 | 23163874 |
| cytotoxicity of bacterial metabolic products, including listeriolysin o, on leukocyte targets. | bacterial toxins can exhibit anticancer activities. here we investigated the anticancer effects of the listeriolysin o toxin produced by listeria monocytogenes. we found that supernatants of listeria monocytogenes strains (wild type, 1189, and 1190) were cytotoxic to the jurkat cell line and human peripheral blood mononuclear cells (pbmc) in a concentration-dependent manner. the supernatant of strain 1044, not producing listeriolysin o, was inactive. the supernatants of listeria strains were als ... | 2012 | 23091365 |
| photocycle of the lov-stas protein from the pathogen listeria monocytogenes. | listeria monocytogenes, a food-borne bacterial pathogen causing significant human mortality, propagates by expressing genes in response to environmental signals, such as temperature and ph. listeria gene (lmo0799) encodes a protein homologous to the bacillus subtilis ytva, which has a flavin-light, oxygen or voltage (lov) domain and a sulfate transporters anti-sigma factor antagonist (stas) output domain that regulates transcription-initiation factor sigma b in the bacterial stress response upon ... | 2013 | 23025752 |
| isolation of a bacteriocin-producing lactococcus lactis and application of its bacteriocin to manage spoilage bacteria in high-value marine fish under different storage temperatures. | the bacteriocins of lactic acid bacteria have considerable potential for biopreservation. the lactococcus lactis strain psy2 (genbank account no. jf703669) isolated from the surface of marine perch perca flavescens produced antibacterial activity against pathogenic and spoilage-causing gram-positive and gram-negative bacteria viz. arthrobacter sp., acinetobacter sp., bacillus subtilis, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa and staphylococcus aureus and possessed broad ... | 2012 | 22555500 |
| expression of virulence genes by listeria monocytogenes j0161 in natural environment. | majority of studies concerning the gene expression of listeria monocytogenes have been done on pure culture states. our objective was to study l.monocytogenes in a co-cultured state and to understand if microbes in their natural state of existence are different in their expression than that of the purely cultured lab grown forms. for a long period discussions have been on the expression of prfa, (which is a virulence gene regulator) in a mammalian host and its role in causing the switch from a s ... | 2012 | 24031897 |
| purification of antilisterial peptide (subtilosin a) from novel bacillus tequilensis fr9 and demonstrate their pathogen invasion protection ability using human carcinoma cell line. | this study focuses on isolation, screening, and characterization of novel probiotics from gastrointestinal tract of free-range chicken (gallus gallus domesticus). fifty seven colonies were isolated and three isolates (fr4, fr9, and fr12) were selected and identified as lactobacillus gasseri fr4, bacillus tequilensis fr9, and l. animalis fr12 by 16s rrna sequencing. three strains were able to survive in stimulated acidic and bile conditions and inhibit the growth of pathogens. especially, fr9 exh ... | 2016 | 27990138 |
| microbiology of cooked and dried edible mediterranean field crickets (gryllus bimaculatus) and superworms (zophobas atratus) submitted to four different heating treatments. | to increase the shelf life of edible insects, modern techniques (e.g. freeze-drying) add to the traditional methods (degutting, boiling, sun-drying or roasting). however, microorganisms become inactivated rather than being killed, and when rehydrated, many return to vegetative stadia. crickets (gryllus bimaculatus) and superworms (zophobas atratus) were submitted to four different drying techniques (t1 = 10' cooking, 24 h drying at 60℃; t2 = 10' cooking, 24 h drying at 80℃; t3 = 30' cooking, 12 ... | 2017 | 27235993 |
| short communication: homologous expression of recombinant and native thurincin h in an engineered natural producer. | the bacillus bacteriocin thurincin h exhibits a wide inhibitory spectrum of activity against various foodborne pathogens, such as listeria monocytogenes, and dairy spoilage bacteria, especially different bacillus species commonly existing in dairy products. previously, we constructed 3 plasmids to express native thurincin h homologously in an engineered natural producer, bacillus thuringiensis sf361thnh(-). this host is deficient in thurincin h production because of an in-frame deletion of struc ... | 2014 | 24792808 |
| heterologous expression of thuricin cd immunity genes in listeria monocytogenes. | bacteriocins are ribosomally synthesized peptides that can have a narrow or broad spectrum of antimicrobial activity. bacteriocin producers typically possess dedicated immunity systems that often consist of an atp-binding cassette (abc) transporter system and/or a dedicated immunity protein. here we investigated the genes responsible for immunity to thuricin cd, a narrow-spectrum two-peptide sactibiotic produced by bacillus thuringiensis dpc6431. heterologous expression of putative thuricin cd i ... | 2014 | 24709257 |
| mega-nano detection of foodborne pathogens and transgenes using molecular beacon and semiconductor quantum dot technologies. | signature molecules derived from listeria monocytogenes, bacillus thuringiensis, and salmonella typhimurium were detected directly on food substrates (mega) by coupling molecular beacon technology utilizing fluorescent resonance energy transfer (fret), luminescent nanoscale semiconductor quantum dots, and nanoscale quenchers. we designed target dna sequences for detecting hlya, bt cry1ac, and inva genes from l. monocytogenes, b. thuringiensis and salmonella typhimurium, respectively, and prepare ... | 2013 | 23722479 |
| ifn-β expression is directly activated in human neutrophils transfected with plasmid dna and is further increased via tlr-4-mediated signaling. | upon lps binding, tlr4 activates a myd88-dependent pathway leading to the transcriptional activation of proinflammatory genes, as well as a myd88-independent/trif-dependent pathway, responsible for the transcriptional induction of ifn-β. previous findings delineated that human neutrophils are unable to induce the transcription of ifn-β in response to tlr4 stimulation. because neutrophils do not express protein kinase c ε, a molecule recently reported as essential for initiating the myd88-indepen ... | 2012 | 22730532 |
| production of bacteriocin-like inhibitory substance by bifidobacterium lactis in skim milk supplemented with additives. | bacteriocins are natural compounds used as food biopreservatives instead of chemical preservatives. bifidobacterium animalis subsp. lactis (bifid. lactis) was shown to produce a bacteriocin-like inhibitory substance (blis) able to inhibit the growth of listeria monocytogenes selected as an indicator microorganism. to enhance this production by the strain bifid. lactis bl 04, skim milk (sm) was used as a fermentation medium either in the presence or in the absence of yeast extract, tween 80 or in ... | 2015 | 25850555 |
| strain-specific probiotic properties of lactic acid bacteria and their interference with human intestinal pathogens invasion. | one of the working mechanisms of probiotic bacteria is their ability to compete with pathogens. to define the probiotic properties of seven lactic acid bacteria (lab) strains, we tested them for survival in simulated gastro-intestinal conditions, antimicrobial activities, co-aggregative abilities, and interferences studies against five human intestinal pathogens (salmonella enteritidis atcc 13076, listeria monocytogenes atcc 7644, escherichia coli o157: h7 atcc 35150, cronobacter sakazakii atcc ... | 2017 | 28286570 |
| mutual growth-promoting effect between bifidobacterium bifidum wbbi03 and listeria monocytogenes cmcc 54001. | in this paper, bifidobacterium bifidum wbbi03 and listeria monocytogenes cmcc 54001 were selected to detect the changes in their growth pattern after mutual interaction between them. the proteomic changes after the interaction between the 2 bacteria were detected by the isobaric tags for relative and absolute quantitation method. the proteins related to the biosynthesis and cell reproduction were selected, and their changes at the transcriptional level were monitored by fluorescent quantitative ... | 2017 | 28259400 |
| eugenol in combination with lactic acid bacteria attenuates listeria monocytogenes virulence in vitro and in invertebrate model galleria mellonella. | listeria monocytogenes is a human enteric pathogen that causes severe foodborne illness in high-risk populations. crossing the intestinal barrier is the first critical step for listeria monocytogenes infection. therefore, reducing l. monocytogenes colonization and invasion of intestinal epithelium and production of virulence factors could potentially control listeriosis in humans. this study investigated the efficacy of sub-inhibitory concentration (sic) of the plant-derived antimicrobial eugeno ... | 2016 | 27002648 |
| isolation, identification and characterisation of three novel probiotic strains (lactobacillus paracasei cncm i-4034, bifidobacterium breve cncm i-4035 and lactobacillus rhamnosus cncm i-4036) from the faeces of exclusively breast-fed infants. | the aim of the present study was to isolate, identify and characterise novel strains of lactic acid bacteria and bifidobacteria with probiotic properties from the faeces of exclusively breast-fed infants. of the 4680 isolated colonies, 758 exhibited resistance to low ph and tolerance to high concentrations of bile salts; of these, only forty-two exhibited a strong ability to adhere to enterocytes in vitro. the identities of the isolates were confirmed by 16s ribosomal rna (rrna) sequencing, whic ... | 2013 | 23360881 |
| application of protective cultures against listeria monocytogenes and campylobacter jejuni in chicken products packaged under modified atmosphere. | to control the growth, or reduce the numbers, of food pathogens such as listeria monocytogenes and campylobacter jejuni in chicken products packaged under modified atmosphere (map), the effectiveness of protective cultures was evaluated in this study. leuconostoc pseudomesenteroides pck18 reduced the counts of l. monocytogenes by 1.22 log cfu/g in chicken burgers under map after 24 d. furthermore, a reduction of 1.16 log cfu/g in c. jejuni together with a delay in the growth of lactic acid bacte ... | 2013 | 23472035 |
| differential expression of virulence and stress fitness genes during interaction between listeria monocytogenes and bifidobacterium longum. | bifidobacterium is well known to have an inhibitory effect on the survival, growth, and proliferation of various foodborne pathogens, but the mechanism of the molecular action of b. longum in blocking the invasion of listeria monocytogenes is not yet well defined. in the present study, following rna extraction and cdna synthesis, differential expression of virulence and stress fitness genes in l. monocytogenes and b. longum was determined by real-time pcr. the results indicate that l. monocytoge ... | 2012 | 22484935 |
| [encephalomeningitis caused by listeria monocytogenes in patient infected by tbe virus--case report]. | the epidemiology of listeriosis is constantly changing towards higher incidence. the most endangered group of patients are people with immunodeficiency caused by coexisting diseases. in these cases listeriosis may take a very severe course. in this paper we present a case of a 76 year old female who suffered from encephalomeningitis caused by listeria monocytogenes and additionally was infected by tick borne encephalitis virus and borrelia burgdorferi. | 2008 | 22320051 |
| antibacterial and antifungal activity of photoactivated zno nanoparticles in suspension. | antibacterial activity of photoactivated zinc oxide nanoparticles (zno nps) against human pathogens escherichia coli o157:h7, listeria monocytogenes atcl3c 7644 and plant pathogen botrytis cinerea was investigated. data indicate that photoactivated (λ = 400 nm) zno nps at concentration 1 × 10(-3)m and incubation time 60 min reduced population of both bacteria by 7 log (cfu/ml). clear dependence of antimicrobial properties of zno nps on used concentration and incubation time was found. scanning e ... | 2013 | 24035847 |
| association of histophilus somni with spontaneous abortions in dairy cattle herds from brazil. | this study investigated the participation of infectious agents in spontaneous abortions and reproductive problems at eight dairy cattle herds from three geographical regions of brazil. fourteen aborted fetuses and the organ sections of one cow with history of repeated abortions were received for pathological evaluations and molecular diagnostics. pcr/rt-pcr assays targeted specific genes of abortifacient agents of cattle: bovine viral diarrhea virus (bvdv), bovine herpesvirus 1 (bohv-1), listeri ... | 2015 | 25480485 |