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plasmid-mediated multiple antibiotic resistance in proteus mirabilis isolated from patients with urinary tract infection.worldwide surveillance of antimicrobial resistance among urinary tract pathogens is useful to determine important trends and geographical variation for common gram negative and positive species. we initiated this study to learn the drug resistance pattern and its location (genomic or plasmid-borne) among the strains of proteus mirabilis, one of the most common causative agents of urinary tract infection (uti).200415507850
detection of ticarcillin-clavulonic acid susceptibility with microdilution method in citrobacter, hafnia, proteus and some gram negative bacteria.the broth dilution method has been regarded as a good alternative test for detection of susceptibilities to antimicrobial agents. in this study, the antimicrobial activity of ticarcillin-clavulonic acid (tim) was investigated by the minimal inhibitory concentration (mic) method on strains of aeromonas, citrobacter, hafnia, morganella, proteus, pseudomonas and gram negative bacteria isolated from raw milk. the isolate collection included 91 gram negative strains. fifty-one (56.04%) isolates were ...200415508433
rapidly rising prevalence of nosocomial multidrug-resistant, gram-negative bacilli: a 9-year surveillance study.to examine and quantify the temporal trends of nosocomial multidrug-resistant, gram-negative bacilli.200415518026
new structures of the o-specific polysaccharides of proteus. 4. polysaccharides containing unusual acidic n-acyl derivatives of 4-amino-4,6-dideoxy-d-glucose.the structures of the o-polysaccharides of the lipopolysaccharides of proteus mirabilis o7 and o49 were determined by chemical methods, mass spectrometry, including ms/ms, and nmr spectroscopy, including experiments run in an h2o/d2o mixture to reveal correlations for nh protons. the o-polysaccharides were found to contain n-carboxyacetyl (malonyl) and n-(3-carboxypropanoyl) (succinyl) derivatives of 4-amino-4,6-dideoxyglucose (4-amino-4-deoxyquinovose, qui4n), respectively. the behavior of qui4 ...200415521818
[chronic carriers of pathogen conjunctival bacteria. possible risks in cataract surgery].to ascertain the frequency and characteristics of chronic carriers of conjunctival pathogen bacteria among patients undergoing cataract surgery in our hospital, to allow the design of studies of their postsurgical endophthalmitis risk.200415523569
the bacteriology of open fractures in ile-ife, nigeria.the objective of this study is to determine the pattern of bacterial isolates and antibiotic sensitivity profile in open fractures. fifty-nine patients with open fractures of the lower limb long bones were studied. the majority of the cases were gustilo and anderson types ii 21(35.6%), and iiia 16(27.1%).200415523862
antimicrobial effect of water extract of sumac (rhus coriaria l.) on the growth of some food borne bacteria including pathogens.the antimicrobial effect of water extracts of sumac (rhus coriaria l.) at concentrations of 0.1%, 0.5%, 1.0%, 2.5% and 5.0% (w/v), non-neutralized and after neutralization to ph 7.2+/-0.1, was studied on the growth of 12 bacterial strains (six gram positive strains and six gram negative strains), mostly food borne including pathogens. it was found to be effective against all the test organisms with gram positive strains being more sensitive than gram negative strains. significant differences (p< ...200415527919
intestinal microflora in 45 crows in ueno zoo and the in vitro susceptibilities of 29 escherichia coli isolates to 14 antimicrobial agents.microorganisms from 45 jungle crows (corvus macrorhynchos) captured from july to december 2002 at ueno zoo, tokyo were identified as escherichia coli, proteus mirabilis, klebsiella pneumoniae, klebsiella oxytoca, enterobacter aerogenes, enterobacter cloacae, enterobacter agglomerans, pseudomonas maltophila, staphylococcus spp., micrococcus spp., and streptococcus spp. e. coli showed the highest rate of isolation (21.6%). in an in vitro susceptibility test for 29 isolates of e. coli to 14 antimic ...200415528866
effect of ceftazidime, amikacin and ciprofloxacin on biofilm formation by some enterobacterial clinical isolates.the effect of sub-minimal inhibitory concentrations of antimicrobial agents on the biofilm formationto polystyrene by escherichia coli, proteus vulgaris, providencia stuartii, and morganella morganii was investigated by examining eight clinical strains. all the isolates tested were efficient biofilm-forming strains in the microtiter plate assay, with crystal violet staining (od595 nm) ranging from 0.13 +/- 0.01 for p. stuartii er21870 to 1.23 +/- 0.02 for p. vulgaris er50120. the biofilm formati ...200415528892
endophthalmitis after pars plana vitrectomy: incidence, causative organisms, and visual acuity outcomes.to investigate the incidence, causative organisms, and visual acuity outcomes associated with endophthalmitis after pars plana vitrectomy.200415531315
genetic and physiological interactions in the amoeba-bacteria symbiosis.amoebae of the xd strain of amoeba proteus that arose from the d strain by spontaneous infection of legionella-like x-bacteria are now dependent on their symbionts for survival. each xd amoeba contains about 42,000 symbionts within symbiosomes, and established xd amoebae die if their symbionts are removed. thus, harmful infective bacteria changed into necessary cell components. as a result of harboring x-bacteria. xd amoebae exhibit various physiological and genetic characteristics that are diff ...200415537083
bacterial isolates in the sick young infant in ile-ife, nigeria.this prospective study was carried out to determine the bacterial pathogens and their antibiotic sensitivity profile in the sick young infant. all consecutive young infants with features suggestive of infection seen over 5 months at a comprehensive health centre in ile-ife, were screened for septicaemia and local bacterial infections. of the 121 sick young infants screened for infection, 94 (77.7 per cent) had confirmed bacterial infection and 54 (57.4 per cent) of the 94 had confirmed septicaem ...200415537715
[infectious spondylodiscitis in a health area of galicia (spain) from 1983-2003].to study the clinical-epidemiological characteristics of infectious spondylodiscitis (is) in adults of our health area in the late 20 years.200415538902
nosocomial outbreak by proteus mirabilis producing extended-spectrum beta-lactamase veb-1 in a korean university hospital.to examine the molecular mechanisms involved in the beta-lactam resistance of multidrug-resistant proteus mirabilis isolates that showed an unusual synergy between imipenem and ceftazidime in a korean hospital.200415546971
craniofacial hyperostoses in proteus syndrome -- a case report.description of a patient with a rare syndrome leading to the partial overgrowth of craniofacial bones.200415555524
beta-lactam susceptibilities and prevalence of esbl-producing isolates among more than 5000 european enterobacteriaceae isolates.in vitro susceptibility to 15 beta-lactam antibiotics was evaluated using enterobacteriaceae isolated during the sentry antimicrobial surveillance program. piperacillin/tazobactam was the most active penicillin against escherichia coli, proteus mirabilis, klebsiella oxytoca and klebsiella pneumoniae (94.9%, 98.3%, 87.4% and 82.9% of isolates susceptible). of the cephalosporins, cefepime was most effective against escherichia coli, proteus mirabilis and enterobacter cloacae (99.2%, 96.3% and 95.2 ...200415555882
modulation of swarming and virulence by fatty acids through the rsba protein in proteus mirabilis.after sensing external signals, proteus mirabilis undergoes a multicellular behavior called swarming which is coordinately regulated with the expression of virulence factors. here we report that exogenously added fatty acids could act as signals to regulate swarming in p. mirabilis. specifically, while oleic acid enhanced swarming, some saturated fatty acids, such as lauric acid, myristic acid, palmitic acid, and stearic acid, inhibited swarming. we also found that expression of hemolysin, which ...200415557604
mannose-resistant proteus-like fimbriae are produced by most proteus mirabilis strains infecting the urinary tract, dictate the in vivo localization of bacteria, and contribute to biofilm formation.proteus mirabilis, an etiologic agent of complicated urinary tract infections, expresses mannose-resistant proteus-like (mr/p) fimbriae whose expression is phase variable. here we examine the role of these fimbriae in biofilm formation and colonization of the urinary tract. the majority of wild-type p. mirabilis cells in transurethrally infected mice produced mr/p fimbriae. mutants that were phase-locked for either constitutive expression (mr/p on) or the inability to express mr/p fimbriae (mr/p ...200415557655
use of translational fusion of the mrph fimbrial adhesin-binding domain with the cholera toxin a2 domain, coexpressed with the cholera toxin b subunit, as an intranasal vaccine to prevent experimental urinary tract infection by proteus mirabilis.this is a follow-up to our previous study using an intranasal vaccine composed of mrph, the tip adhesin of the mr/p fimbria, and cholera toxin to prevent urinary tract infection by proteus mirabilis (x. li, c. v. lockatell, d. e. johnson, m. c. lane, j. w. warren, and h. l. mobley, infect. immun. 72:66-75, 2004). here, we have expressed a cholera toxin-like chimera in which the mrph adhesin-binding domain (residues 23 to 157) replaces the cholera toxin a1 adp-ribosyltransferase domain. this chim ...200415557656
[delayed brain abscess as a complication of a csf shunt].infection is a common complication of ventriculoperitoneal shunts, but the formation of a brain abscess related to shunt system is very rare. we present the case of a patient who developed a brain abscess around a ventricular catheter left in place after removing the valvular system.200415558205
phylogenetic characterization of legionella-like endosymbiotic x-bacteria in amoeba proteus: a proposal for 'candidatus legionella jeonii' sp. nov.the x-bacteria which initiated organismic association with the d strain of amoeba proteus in 1966 as parasites have changed to obligate endosymbionts on which the host depends for survival. owing to the difficulty in cultivating the bacteria in vitro, the identity of x-bacteria has not been determined. as the life cycle of x-bacteria is similar to that of legionella spp. in soil amoebae, we applied the polymerase chain reaction method with specific primers aimed at legionella spp. for the detect ...200415560823
cockroaches as potential vectors of nosocomial infections.to determine the possible role of cockroaches in the epidemiology of nosocomial infections.200415566034
lanthanide spectroscopic studies of the dinuclear and mg(ii)-dependent pvuii restriction endonuclease.type ii restriction enzymes are homodimeric systems that bind four to eight base pair palindromic recognition sequences of dna and catalyze metal ion-dependent phosphodiester cleavage. while mg(ii) is required for cleavage in these enzymes, in some systems ca(ii) promotes avid substrate binding and sequence discrimination. these properties make them useful model systems for understanding the roles of alkaline earth metal ions in nucleic acid processing. we have previously shown that two ca(ii) i ...200415568821
medical image. flank football. 200415570703
"craniofacial and dental manifestations of a case of proteus syndrome".proteus syndrome, which is a reference to the ancient greek god proteus, the polymorphous, was called to the attention of pediatricians. recognition of this syndrome has been difficult because of the variability of the syndrome's manifestations and because of its rarity. we describe a rare case of proteus syndrome and we discuss its differential diagnosis. our case presents with hemi facial hypertrophy, scrotal tongue, enamel hyperplasia as well as differential in the size of the dentition on bo ...200415573665
clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle.bacteria contaminate the uterus of most dairy cattle after parturition and endometritis causes infertility. an endometritis score can be ascribed based on the vaginal mucus character and odour but it is not clear if the clinical score reflects the number of uterine bacteria or the inflammatory response. the present study tested the hypothesis that clinical evaluation of endometritis reflects the number of bacteria present in the uterus, and the acute phase protein response. swabs (n = 328) were ...200515589277
does the valve regulated release of urine from the bladder decrease encrustation and blockage of indwelling catheters by crystalline proteus mirabilis biofilms?we tested whether valve regulated, intermittent flow of urine from catheterized bladders decreases catheter encrustation.200515592093
synthesis and study of antibacterial and antifungal activities of novel 2-[[(benzoxazole/benzimidazole-2-yl)sulfanyl] acetylamino]thiazoles.several 2-[[(benzoxazole/benzimidazole-2-yl)sulfanyl]acetylamino]thiazoles derivatives were synthesized by reacting 4-substituted-2-(chloroacetylamino)thiazoles with benzoxazole/benzimidazole-2-thioles in acetone and in the presence of k2co3. the chemical structures of the compounds were elucidated by ir, 1h-nmr, and fab(+)-ms spectral data. their antimicrobial activities against micrococcus luteus (nrll b-4375), bacillus cereus (nrrl b-3711), proteus vulgaris (nrrl b-123), salmonella typhimuriu ...200415595406
vascular anomalies and the growth of limbs: a review.growth of the limb in a child can be impaired, with the coexistence of a vascular malformation. in these vascular bone syndromes, altered growth is manifest as overgrowth or hypotrophy. the vascular malformation is usually complex and gets progressively worse with time. the two types of vascular anomalies in limbs, fast-flow and slow-flow, can be associated with limb length discrepancies. the fast-flow vascular malformations together with arteriovenous fistulae are part of parkes weber syndrome, ...200415599224
[relationship between antibacterial activity of aloe and its anthaquinone compounds].to investigate the relationship between the antibacterial activity of aloe and its contents of anthaquinone compounds, measure and compale antibacterial activities of aloin and aloe-emodin, and analyse the effect of glycoside on the antibacterial activity of aloin.200315615409
[effect of delay of blood cultures on positive detection by automated blood culture system].the effect of entry delayed blood culture bottles until the start of incubation for mechanical detection of organism were compared using 2 major blood culture systems; bactec 9240 system and bact/alert 3d system. total of 13 bacterial strains; 5 gram-positive cocci, 7 gram-negative bacilli and candida parapsilosis which were isolated mainly from blood cultures were used as the test strains. bactec 92f, 93f and bact/alert fa, fn bottles were used as the blood culture bottles. all the bottles inoc ...200415628528
evaluation of a new screen agar plate for detection and presumptive identification of enterobacteriaceae producing extended-spectrum beta-lactamases.a new agar screen plate for extended-spectrum beta-lactamase (esbl) detection was evaluated with 50 clinical isolates of esbl-producing enterobacteriaceae species: enterobacter cloacae (n = 10), escherichia coli (n = 10), klebsiella oxytoca (n = 3), klebsiella pneumoniae (n = 25), and proteus mirabilis (n = 2). fecal samples were artificially inoculated with 2 concentrations (25 and 250 colony forming units [cfu]/plate) of the test strains and then applied to the new agar screen plates. by this ...200515629229
nature of the promoter activated by c.pvuii, an unusual regulatory protein conserved among restriction-modification systems.a widely distributed family of small regulators, called c proteins, controls a subset of restriction-modification systems. the c proteins studied to date activate transcription of their own genes and that of downstream endonuclease genes; this arrangement appears to delay endonuclease expression relative to that of the protective methyltransferase when the genes enter a new cell. c proteins bind to conserved sequences called c boxes. in the pvuii system, the c boxes have been reported to extend ...200515629920
intracranial complications of otitis media: 15 years of experience in 33 patients.complications in the central nervous system (cns) from acute otitis media (aom) and chronic otitis media (com) are becoming fewer, although they still represent a challenge for early recognition, adequate treatment, and satisfactory results. this retrospective study analyzed clinical data and therapeutic options in 33 patients with intracranial involvement resulting from otitis media. important clinical features of intracranial complications and the sequence of the most efficient therapeutic man ...200515632907
microbial resistance to antibiotics used to treat urinary tract infections in mexican children.urinary tract infections are one of the most common bacterial infections in female children. this infection has a prevalence of 4.1 to 7.1% during infancy. the bacterial agents causing the urinary tract infection are escherichia coil (80-90%), followed by other gram negative germs such as klebsiella pneumoniae and enterococcus species and gram positive such as staphylococcus species. typical treatment for this infection is ampicillin or the mixture of trimethoprim and sulfamethoxazole (tmt/smx) ...200415633630
[naso-subcutaneous application of the polycomponent vaccine vp-4 for the treatment of patients with bronchial asthma and chronic obstructive bronchitis].bacterial polycomponent vaccine (vp-4) earlier developed at the mechnikov research institute for vaccines and sera on the basis of antigenic complexes of klebsiella pneumoniae, proteus vulgaris, escherichia coli, staphylococcus aureus was used within the program of clinical trials, for the treatment of 30 patients aged 16-59 years with the atopic and mixed form of bronchial asthma and chronic obstructive bronchitis by the combined naso-subcutaneous application. a good therapeutic effect produced ...200415636137
[bacterial causes of meningitis in newborns].bacterial meningitis jeopardizes the life of affected newborns and in survived children often leaves permanent sequels. the sooner the diagnosis is established and the therapy is started, the more a prosperous outcome can be expected. when the indication is established, lumbar punction should be performed. on the basis of the results of cytological and biochemical analyses of the cerebrospinal fluid (csf) that point to bacterial meningitis, the initial therapy should begin even before the result ...200115637989
rapid, species-specific detection of uropathogen 16s rdna and rrna at ambient temperature by dot-blot hybridization and an electrochemical sensor array.development of rapid molecular approaches for pathogen detection is key to improving treatment of infectious diseases. for this study, the kinetics and temperature-dependence of dna probe hybridization to uropathogen species-specific sequences were examined. a set of oligonucleotide probes were designed based on variable regions of the 16s gene of the escherichia coli, proteus mirabilis, klebsiella oxytoca, and pseudomonas aeruginosa. a universal bacterial probe and probes-specific for gram-posi ...200415639199
enhanced biofilm-production in pathogens isolated from patients with rare metabolic disorders.biofilm-producing bacteria were isolated from the urine of 19 patients with very rare metabolic disorders including: hyperlactacidaemia (8 cases), sugar intolerance and gammopathy (1 case), cystinuria (2 cases), parkinson's disease (1 case), lipidaemia (2 cases), hyperaminoaciduria (1 case) and others (4 cases). a total of 34 strains were collected, gram-negative and gram-positive microorganisms were equally distributed among the slime-producing bacteria, with a prevalence of staphylococcus epid ...200415646050
antiviral and antimicrobial activities of new nitrobutane derivatives.it is known that some addition products of beta-nitrostyrenes exhibit potent antimicrobial activity. in order to investigate the effects of structural modifications on the biological properties, some new michael type addition products of beta-ethyl-beta-nitrostyrenes were synthesized. in this study, eight new 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane (2) derivatives were synthesized by addition of 2-aminothiophenol to the double bond of beta-ethyl-beta-nitrostyrenes (1). the chemical struct ...200415646374
influence of various criteria for elimination of duplicates when calculating the prevalence and antibiotic susceptibility of microorganisms associated with urinary infections.the effect of applying various criteria for elimination of repeated isolates in the same patient on the prevalence and antibiotic susceptibility of microorganisms frequently associated with urinary infections were analyzed. when time criteria were applied, there was a decrease in the number of isolates of 13.4-27.5%, whereas when variations in antibiotic susceptibility were considered, between 4.3 and 20.3% of the isolates were eliminated. the absolute number of isolates varied considerably depe ...200515664489
functional characterization of contractile vacuole isolated from amoeba proteus.contractile vacuoles (cvs) released from cells of amoeba proteus were used to analyze its function in vitro. when cv was transferred to a hypertonic medium, its volume decreased within 10 sec. when it was subsequently returned to its original medium, it quickly started swelling. however, it ruptured before recovering its initial volume. these results suggested that the cv membrane is semi-permeable and that the fluid is collected by the osmotic gradient in vivo. the water permeability of membran ...200415665503
antimicrobial activity of sesquiterpenes from the essential oil of juniperus thurifera.chemical analysis of the essential oil of juniperus thurifera wood led to the identification of fifty-eight sesquiterpene compounds, of which twenty-eight have been isolated, two of them being new natural cedrane derivatives (1 and 2). their structures were established on the basis of 1d nmr and 2d nmr spectra. the antimicrobial activities of different pure compounds of this oil were tested against nine microorganisms (gram-positive and gram-negative bacteria, and yeasts). seven of the tested co ...200515678376
structure and serological studies of the o-polysaccharide of proteus penneri 75 epitopes and subgroups of proteus serogroup o73.the o-specific polysaccharide of the lipopolysaccharide of proteus penneri strain 75 consists of tetrasaccharide-ribitol phosphate repeating units and resembles ribitol teichoic acids of gram-positive bacteria. the following structure of the polysaccharide was elucidated by chemical methods and 1h and 13c nmr spectroscopy: [structure in text] where rib-ol is ribitol. serological studies with polyclonal antisera showed that the same structure of the o-polysaccharide occurred in two strains: p. pe ...200515681143
intracranial complications following mastoidectomy.mastoidectomy is a common surgical procedure in otology. however, postoperative complications of various degrees of severity may occur. we present 4 children who underwent mastoidectomy for middle ear and mastoid disease and developed postoperative intracranial complications. one child was operated on for brain abscess 1 week after the initial mastoidectomy. another child appeared with seizures 5 days after the initial mastoidectomy and a subdural empyema was drained during revision surgery. lar ...200415689642
pollution status of swimming pools in south-south zone of south-eastern nigeria using microbiological and physicochemical indices.microbiological and physicochemical characteristics of swimming pools in south eastern states of nigeria (akwa ibom and cross river) were investigated. the bacterial isolates included enterococcus faecalis, clostridium perfringens, bacillus cereus and escherichia coli. others were pseudomonas aeruginosa, staphylococcus aureus, proteus vulgaris and staphylococcus epidermidis, while fungal isolates were penicillium sp, rhizopus sp, aspergillus versicolor fusarium sp, trichophyton mentagrophytes, m ...200415691161
chondroitinase abc i from proteus vulgaris: cloning, recombinant expression and active site identification.galags (galactosaminoglycans) are one subset of the gag (glycosaminoglycan) family of chemically heterogeneous polysaccharides that are involved in a wide range of biological processes. these complex biomacromolecules are believed to be responsible for the inhibition of nerve regeneration following injury to the central nervous system. the enzymic degradation of gag chains in damaged nervous tissue by cabc i (chondroitinase abc i), a broad-specificity lyase that degrades galags, promotes neural ...200515691229
longitudinal trends in antimicrobial susceptibilities across long-term-care facilities: emergence of fluoroquinolone resistance.antibiotic resistance in the longterm-care facility (ltcf) setting is of increasing concern due to both the increased morbidity and mortality related to infections in this debilitated population and the potential for transfer of resistant organisms to other healthcare settings. longitudinal trends in antibiotic resistance in ltcfs have not been well described.200515693409
[distribution and drug-resistance of 3 500 gram-negative bacteria in guangzhou].to investigate the distribution and drug-resistance of the common gram-negative bacteria in guangzhou.200515698988
[etiology and antimicrobial susceptibility among uropathogens causing community-acquired lower urinary tract infections: a nationwide surveillance study].knowledge of antimicrobial susceptibility patterns is required to prescribe empirical therapy and formulate guidelines for the treatment of community-acquired urinary tract infections. this multicenter study assesses the etiology and antimicrobial susceptibility of the main community-acquired uropathogens in spain.200515701325
otomycoses of candidal origin in eastern slovakia.mycological analysis of swabs and scraping samples from the external ear canals of 40 patients with clinically diagnosed otomycosis (10 neonates, 30 adults) revealed the presence of fungi as etiological agents. they were investigated microscopically using 20 % potassium hydroxide, and by cultivation on sabouraud's glucose agar. the candida species were identified using the germ-tube test, micromorphology observations of colonies on rice agar, and particularly by the commercial kit auxacolor. the ...200415702553
structure of the o-polysaccharide and serological studies of the lipopolysaccharide of proteus penneri 60 classified into a new proteus serogroup o70.an alkali-treated lipopolysaccharide of proteus penneri strain 60 was studied by chemical analyses and 1h, 13c and 31p nmr spectroscopy, and the following structure of the linear pentasaccharide-phosphate repeating unit of the o-polysaccharide was established: 6)-alpha-d-galp-(1-->3)-alpha-l-fucpnac-(1-->3)-alpha-d-glcpnac-(1-->3)-beta-d-quip4nac-(1-->6)-alpha-d-glcp-1-p-(o--> rabbit polyclonal o-antiserum against p. penneri 60 reacted with both core and o-polysaccharide moieties of the homologo ...200515708308
isolation of bacteria with antibiotic resistance from household cockroaches (periplaneta americana and blattella germanica).cockroaches may harbor and disseminate microorganisms to the environment. in this study, periplaneta americana and blattella germanica were collected from 40 households in kaohsiung city and kaohsiung county, taiwan. cockroach infestation was found in 50% of the studied households and 226 cockroaches (123 p. americana and 103 b. germanica) collected by trapping. p. americana was more often found in the kitchen (70.7%) whereas b. germanica in the storage room (51.5%) and kitchen (36.9%). there wa ...200515716054
pharmacodynamics of ibafloxacin in micro-organisms isolated from cats.the pharmacodynamic properties of ibafloxacin were investigated in micro-organisms isolated from cats. minimal inhibitory concentrations (mic) of ibafloxacin (racemate, r- and s-enantiomers) and its metabolites (7-hydroxy- and 8-hydroxy-ibafloxacin) and time-kill kinetics were determined against gram-negative and gram-positive bacteria isolated from dermal and respiratory and urinary tract infections in cats. racemic ibafloxacin has a broad spectrum of bactericidal activity against gram-negative ...200515720512
acute recurrent diverticulitis is prevented by oral administration of a polybacterial lysate suspension.the main cause of acute diverticulitis is the abnormal accumulation of fecal bacteria within the diverticular lumen, leading to a balancing between normal probiotic microflora and pathogenic species; gram negative entero-bacteriaceae, mainly escherichia coli and proteus spp, are the genders that usually cause the disease-related symptoms, due to their ability to adhere to intestinal mucosa. the intestine is well known as the largest human lymphoepithelial organ and daily produces more antibodies ...200415722985
rho/rho-dependent kinase affects locomotion and actin-myosin ii activity of amoeba proteus.the highly motile free-living unicellular organism amoeba proteus has been widely used as a model to study cell motility. however, the molecular mechanisms underlying its unique locomotion are still scarcely known. recently, we have shown that blocking the amoebae's endogenous rac- and rho-like proteins led to distinct and irreversible changes in the appearance of these large migrating cells as well as to a significant inhibition of their locomotion. in order to elucidate the mechanism of the rh ...200415726816
survey of the susceptibility of urinary isolates to antibacterial agents in 2003.in 2003, 826 uropathogenic strains were obtained from the urine of our patients. we assessed their activities to antibacterial agents based on the minimal inhibitory concentration (mic) breakpoints recommended by the national committee for clinical standards (nccls). methicillin-resistant strains accounted for about 70% of staphylococcus aureus and staphylococcus epidermidis. the most common gram-positive species was enterococcus faecalis (15.6%). enterococcus faecium was the most resistant of t ...200515729488
identification and functional characterisation of genes and corresponding enzymes involved in carnitine metabolism of proteus sp.enzymes involved in carnitine metabolism of proteus sp. are encoded by the cai genes organised as the caitabcdef operon. the complete operon could be sequenced from the genomic dna of proteus sp. amino acid sequence similarities and/or enzymatic analysis confirmed the function assigned to each protein involved in carnitine metabolism. cait was suggested to be an integral membrane protein responsible for the transport of betaines. the caia gene product was shown to be a crotonobetainyl-coa reduct ...200515731894
susceptibility testing of urinary isolates of escherichia coli to mecillinam using nccls methodology.criteria for susceptibility testing of mecillinam against 533 isolates of escherichia coli and a further 309 enterobacteriaceae, according to nccls methodology, were determined. correlation of mic to inhibition zones was good for all species. for urinary isolates of e. coli, the following agar dilution breakpoints and corresponding interpretive zone diameters seem appropriate: < or = 8 mg/l/> or = 15 mm for susceptible; 16 mg/l/12-14 mm for intermediate susceptible and > or = 32 mg/l/< or = 11 m ...200515737512
[prevalence of proteus mirabilis strains in clinical specimens and evaluation of their resistance to selected antibiotics].proteus mirabilis are opportunistic microorganisms which cause serious hospital infections. the resistance of 1038 strains of p. mirabilis to selected antibiotics (lactams, aminoglycosides, fluoroquinolones, nitrofurantoin, cotrimoxazole, tetracycline) was tested by disk diffusion method. the least of p. mirabilis strains were resistant to imipenem and aztreonam. most of p. mirabilis strains were isolated from urine, wounds and ulcers from patients of departments of urology, dermatology, general ...200415754653
[enzymatic resistance to beta lactam antibiotics within the genus proteus and evaluation of proteus mirabilis phenotypes and genotypes for resistance to third- and fourth-generation cephalosporins].the aim of this study was to evaluate betalactam resistance within the genus proteus and characterize the betalactamases responsible for this resistance.200515757582
interleukin-8 response in cells from the human urinary tract induced by lipopolysaccharides of proteus mirabilis o3 and o18.proteus mirabilis is a common pathogen associated mainly with complicated urinary tract infections and sometimes with septicemia. there is great serological diversity of the microorganism. while p. mirabilis o3 is commonly found in patients with infections, the serotype o18 rarely occurs. the o18 lipopolysaccharide contains a phosphocholine substitute, which makes it unique among proteus strains. to explain different clinical significance of the strains we evaluated the biological activity of th ...200515758809
(1)h nmr spectroscopy in the diagnosis of pseudomonas aeruginosa-induced urinary tract infection.the utility of (1)h nmr spectroscopy is suggested and demonstrated for the diagnosis of pseudomonas aeruginosa in urinary tract infection (uti). the specific property of p. aeruginosa of metabolizing nicotinic acid to 6-hydroxynicotinic acid (6-ohna) is exploited. the quantity of 6-ohna produced correlates well with the viable bacterial count. other common bacteria causing uti such as escherichia coli, klebsiella pneumonia, enterobacter aerogenes, acinetobacter baumanii, proteus mirabilis, citro ...200515759292
chlorine covers on living bacteria: the initial step in antimicrobial action of active chlorine compounds.although active chlorine compounds are well-known antimicrobial agents in human medicine, their initial steps of action have not been completely clarified. using n-chlorotaurine (nct), an endogenous mild representative, we observed persisting oxidation capacity affixed to bacteria. it was the aim of this study to investigate this 'chlorine cover'.200515761074
synthesis of some 1-[(n, n-disubstituted thiocar bamoylthio)acetyl]-3-(2-thienyl)-5-aryl-2-pyrazoline derivatives and investigation of their antibacterial and antifungal activities.fourteen new 1-[(n, n-disubstituted thiocarbamoylthio)acetyl]-3-(2-thienyl)-5-aryl-2-pyrazoline derivatives (7a-n) were synthesised by reacting 1-(chloroacetyl)-3-(2-thienyl)-5-aryl-2-pyrazolines (5a-g) and appropriate sodium salts of n, n-disubstituted dithiocarbamoic acids (6a, b). the structures of the synthesised compounds were confirmed by elemental analyses, uv, ir, (1)h-nmr and fab(+)-ms spectral data. their antibacterial activities against proteus vulgaris (nrrl b-123), escherichia coli ...200515765490
[urinary tract infections in children--etiologic agents and susceptibility to antibiotics].urinary tract infections (utis) are common clinical problem in pediatric hospitals. the aim of this study was to compare the incidence of utis during two different time periods (from february 1999 to march 2000, and from april 2000 to september 2001) in pediatric patients treated in ambulatory or hospitalized in new children hospital. the frequency of occurrence of esbl--producing gram-negative rods increased during second period study. the isolated esbl--producing strains were resistant also to ...200415765758
[hydrolytic activity of marine bacteria associated with the mussel mytilus trossulus].the ability to produce hydrolytic enzymes--dnases, rnases, alkaline phosphatases, chitinases has been studied in 316 strains of bacteria isolated from mussel mytilus trossulus. strains of vibrio spp. produced all the spectrum of examined enzymes, bacteria of pseudoalteromonas genus produced alkaline phosphatase and dnase. chitin-degrading enzymes were found in vibrio and cytophaga--flavobacterium--bacteroides group. bacillus and vibrio spp. strains produced most actively rnases. the presence of ...200515765877
malignant mimickers: chronic bacterial and fungal infections of the larynx.chronic infections of the larynx are notorious "copycats" of squamous cell carcinoma. patients typically present with a historical picture and symptoms identical to those seen in a neoplastic setting: dyspnea, hoarseness, odynophagia, weight loss, and a history of tobacco and alcohol abuse. historically, these patients were subject to an extensive resection for what was in reality a benign disease. a better understanding and awareness of these conditions has reinforced the need for a direct lary ...200515766861
leucocyte migration and nitroblue tetrazolium assay in nigerian children with bacteremia and malaria parasitemia.the prevalence of malaria parasitemia, bacteremia, certain hematological parameters, leucocyte migration index and nitroblue tetrazolium dye reduction were determined in 147 nigerian children (4.24+/-2.88 years of age). sixty (40.8%), 28(19.1%) and 26(17.7%) had malaria parasitemia only, bacteremia only and both malaria parasitemia and bacteremia, respectively. four genera of bacteria, i.e e. coli, proteus, staphylococcus and salmonella, were detected in subjects with both malaria parasitemia an ...200415783134
evaluation of antimicrobial activity of beta-lactam antibiotics using etest against clinical isolates from 60 medical centres in japan.an antimicrobial resistance surveillance study was carried out in 60 medical centres across japan. resistance to piperacillin was 10.8% in clinical isolates of escherichia coli, while 1.3% or fewer isolates were resistant to other beta-lactams. klebsiella spp. were more susceptible to imipenem, cefepime and cefpirome. isolates of enterobacter spp., citrobacter spp., indole-positive proteus and serratia spp. were susceptible to imipenem, cefepime and cefpirome, while acinetobacter spp. were most ...200515784308
the antimicrobial activity of extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent.the antimicrobial activity and the mic values of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent have been investigated against some microorganisms. at least one of the extracts or 3-hydroxyphysodic acid showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus vulgaris, salmonella ...200515787241
[resistance of catheter-associated urinary tract infections to antibacterials].catheter-associated urinary tract infections (cauti) are the most common nosocomial infections. the worldwide data show the increasing resistance to conventional antibiotics among urinary tract pathogens.200515790045
incidental detection of purulent fluid in kidney at percutaneous nephrolithotomy for branched renal calculi.some patients undergoing percutaneous nephrolithotomy (pcnl) have purulent fluid in the pelvicaliceal system at the time of puncture, although preoperative features are not suggestive of infection. we report the management and outcome of 19 such patients.200515798405
subset of individuals with autism spectrum disorders and extreme macrocephaly associated with germline pten tumour suppressor gene mutations.the genetic aetiology of autism remains elusive. occasionally, individuals with cowden syndrome (a cancer syndrome) and other related hamartoma disorders such as bannayan-riley-ruvalcaba syndrome, proteus syndrome, and proteus-like conditions, are characterised by germline pten mutations, and may have neurobehavioural features resembling autism as well as overgrowth and macrocephaly. therefore, we undertook pten gene mutation analysis in 18 subjects mainly prospectively ascertained with autism s ...200515805158
molecular diversity of proteus mirabilis isolates producing extended-spectrum beta-lactamases in a french university hospital.between february 1997 and december 2002, 3340 hospitalised patients yielded samples positive for proteus mirabilis, of whom 45 (1.3%) were colonised/infected by p. mirabilis producing extended-spectrum beta-lactamases (esbls). the gross incidence of patients colonised/infected by esbl-producing p. mirabilis was 1.61/10(5) days of hospitalisation, with 20% of isolates being collected from patients in urology wards, most frequently (53.3%) from urine samples. seventeen (37.7%) of the 43 isolates w ...200515819867
interaction of clindamycin and gentamicin in vitro.the minimal inhibitory concentrations of clindamycin and gentamicin alone and in combinations were determined by a microdilution method for 163 aerobic, facultative, and anaerobic clinical isolates. all 77 strains of staphylococcus aureus, diplococcus pneumoniae, streptococcus pyogenes, and anaerobic bacteria (except for three strains of clostridium) were inhibited by 1.6 mug or less of clindamycin per ml. gentamicin did not interfere with the activity of clindamycin within the range of concentr ...197415825309
synergy between cephalosporin and aminoglycoside antibiotics against providencia and proteus.clinical isolates of providencia and proteus with relative aminoglycoside resistance were tested for susceptibility to combinations of gentamicin or tobramycin with cephalothin or cefazolin. the minimal bactericidal concentration of aminoglycoside for one-third of the strains was reduced by fourfold or more in the presence of one-fourth of the minimal bactericidal concentration of either cephalosporin. this effect was achieved by clinically attainable concentrations of cephalothin or cefazolin.197415825407
potential unreliability of nitrofurantoin disks in susceptibility testing.the kirby-bauer disk diffusion antimicrobial susceptibility test was studied with three lots of commercial nitrofurantoin disks by using 50 strains of proteus mirabilis. one lot of disks consistently gave zone diameters 3 to 6 mm larger than the other two and resulted in a substantial number of proteus mirabilis strains being erroneously categorized as sensitive. antimicrobial content in disks of the three lots did not account for these differences. the ph of eluate from the unreliable disks was ...197415825413
technique for determining the bactericidal effect of drug combinations.two paper strips, each containing different antimicrobial agents, were placed on plates on mueller-hinton agar to permit antibiotic to enter the agar. a filter membrane was placed on this plate, and the microorganisms were planted on the membrane. after 6 h of incubation at 37 c, the membrane was transferred to antibiotic-free mueller-hinton agar containing triphenyltetrazolium hydrochloride and incubated for 18 h at 37 c. specific growth patterns were indicative of additive (indifferent), syner ...197415825416
ossicular discontinuity and exostoses in proteus syndrome: a case report.proteus syndrome (ps) is a rare hamartomatous disorder characterized by mosaic overgrowth of multiple tissues that manifests early in life and is progressive. the presence of unilateral external auditory canal exostoses in a patient who is not a swimmer or surfer is suggestive of ps. however, hearing loss is not a typical feature. here, we describe exostoses and ossicular discontinuity with conductive hearing loss in a patient with ps. the treatment consisted of canalplasty and ossicular chain r ...200515825577
[severe community-acquired pneumonia due to proteus mirabilis]. 200515826556
childhood parapneumonic pleural effusion in enugu.to review the epidemiological pattern and clinical features, management and outcome of childhood parapneumonic pleural effusion in enugu.200515827593
r-mediated beta-lactamases and episomal resistance to the beta-lactam drugs in different bacterial hosts.ten penicillinase plasmids of varying taxonomic origin were studied after transfer to a variety of bacterial hosts. nine of the ten plasmids specified enzymes with the following identical, or very similar, properties: substrate profile, molecular weight, susceptibility to heat and inhibitors, and electrophoretic mobility, i.e., tem-like enzymes. the tenth r-mediated beta-lactamase was a cephalosporinase. plasmids with tem-like enzymes mediated resistance patterns identical towards the beta-lacta ...197415828172
combined activity of sulfamethoxazole, trimethoprim, and polymyxin b against gram-negative bacilli.the activity of the three two-drug combinations of sulfamethoxazole (smx), trimethoprim (tmp), and polymyxin b (pb) against 52 clinical isolates of gram-negative bacilli was studied by a "checkerboard" agar dilution method. the organisms studied included strains of enterobacter spp., klebsiella pneumoniae, serratia marcescens, providence, proteus, and pseudomonas aeruginosa. the majority of these isolates were resistant to at least two of the three agents used in the combined studies and to the ...197415828175
butirosin compared with gentamicin in vitro and in vivo.butirosin (btn) (p. w. k. woo, g. l. coffey, h. w. dion, s. a. fusari, and g. d. senos, u. s. patent 3,541,078, 1970) is a new aminoglycoside antibiotic notably active against opportunist bacterial species within pseudomonas, klebsiella, enterobacter, serratia, and proteus. numerous comparative tests were carried out with btn and gentamicin (gtm) in vitro and in experimental infections in mice. btn was more active in mueller-hinton broth than in agar, but its activity was lessened at acid ph or ...197415828182
cefoxitin, a semisynthetic cephamycin antibiotic: antibacterial spectrum and resistance to hydrolysis by gram-negative beta-lactamases.the in vitro activity of cefoxitin, 3-carbamolyloxymethyl-7-alpha-methoxy-7[2-(2-thienyl)acetamido]-3-cephem-4-carboyxlic acid, was investigated. activity against gram-positive organisms was less than that of cephalothin and cephloridine. it was highly active against gram-negative bacilli, with activity against escherichia coli, proteus mirabilis, and klebsiella pneumoniae equal to that of currently available cephalosporins. in addition, it was active against certain enterobacter strains, serrat ...197415828188
cefamandole, a cephalosporin antibiotic with an unusually wide spectrum of activity.the in vitro activity of cefamandole, 7-d-mandelamido-3-(1 methyl-1h-tetrazol-5 yethiomethyl)-3-cephem-4 carboxylic acid, was investigated. the majority of streptococci and pneumococci were inhibited by 0.1 mug/ml. activity against staphylococcus aureus was below that of cephalothin, but most strains were inhibited by 0.4 mug/ml. enterococci were not inhibited by less than 25 mug/ml. cefamandole was very active against most members of the enterobacteriaceae, with 70% of escherichia coli, 86% of ...197415828189
gram-negative bacterial resistance to cephalosporins in community-acquired infections in turkey.cephalosporins are widely used and trustworthy antibiotics in daily medical practice. although antibacterial resistance has been reported in hospital wards, there are less data for community-acquired infections. in this study we investigated the cephalosporin susceptibility profiles of community-acquired gram-negative bacteria isolates in sivas kizilay medical center (turkey) between march 2002 and march 2003. in our study, 949 escherichia coli, 165 proteus spp., 97 enterobacter spp., 24 klebsie ...200515828445
new antimicrobial nitrofuran, trans-5-amino-3-[2-(5-nitro-2-furyl)vinyl]-delta2-1,2,4-oxadiazole: antimicrobial efficacy in mice, rats, and guinea pigs.a new antimicrobial nitrofuran designated sq 18,506 showed some therapeutic activity when administered orally to mice infected with escherichia coli, salmonella schottmuelleri, shigella flexneri, or klebsiella pneumoniae. animals infected parenterally with streptococcus pyogenes, proteus mirabilis, mycobacterium tuberculosis, and candida albicans, or topically with trichophyton mentagrophytes, did not respond to therapy with the drug at the dosage levels used. the compound was as effective as me ...197415830472
treatment of pneumonia and other serious bacterial infections with cephapirin.nineteen patients with pneumonia due to streptococcus pneumoniae, staphylococcus aureus, klebsiella pneumoniae, or escherichia coli were treated with 4 to 18 g of cephapirin daily. there were three treatment failures. one patient each with pneumonia due to e. coli or s. pneumoniae died despite apparent eradication of the pathogen. lobar pneumonia due to k. pneumoniae progressed during therapy in a third patient to lung gangrene, necessitating pneumonectomy. five additional patients with pneumoco ...197415830473
cefoxitin and cephalothin: antimicrobial activity, human pharmacokinetics, and toxicology.cefoxitin, a semisynthetic cephamycin, has been compared with the widely used parenteral cephalosporin, cephalothin, in terms of antibacterial activity, human pharmacokinetics, and toxicity. for both compounds, minimal inhibitory concentrations were within the therapeutic range against the 156 gram-positive cocci tested (except group d streptococci), but cephalothin was 8 to 20 times more active. regarding the 313 gram-negative organisms tested, both antibiotics were of approximately equal activ ...197415830475
susceptibility of clinical isolates of bacteria to cefoxitin and cephalothin.the susceptibility of 4,929 unselected clinical isolates of bacteria to cefoxitin and cephalothin was determined by the single-disk method, using a computer-associated electronic zone analyzer to obtain, record, and process measurements of sizes of zones of inhibition. both cefoxitin and cephalothin were effective against most gram-positive strains, including staphylococcus aureus, s. epidermidis, micrococci, and all streptococci except enterococci. the three strains of listeria monocytogenes te ...197415830480
effects of rate of infusion and probenecid on serum levels, renal excretion, and tolerance of intravenous doses of cefoxitin in humans: comparison with cephalothin.using a randomized crossover design, 1-g intravenous doses of cephalothin and cefoxitin, a cephalosporinase-resistant cephamycin, were infused into 12 normal adult males over periods of 120, 30, and 3 min, the last with and without prior intravenous infusions of probenecid (1 g). mean peak serum concentrations of antibiotic activity after cephalothin infusions were 23, 56, 103, and 102 mug/ml, respectively, and after cefoxitin infusions they were 27, 74, 115, and 125 mug/ml, respectively. proben ...197415830485
an ethnobotanical survey of bauchi state herbal plants and their antimicrobial activity.a survey of medicinal plants used locally in the treatment of various diseases was carried out in bauchi state-nigeria. a total of 84 medicinal plants were listed. preliminary antimicrobial activity of the aqueous extracts of the plants was investigated. the results indicated that out of 84 plants, 75 exhibited antimicrobial activity against one or more of the test organisms at a concentration of 200 mg/ml. the extracts were found to show potentially interesting activity against proteus mirabili ...200515848012
[in vitro susceptibilites to levofloxacin and various antibacterial agents of 11,475 clinical isolates obtained from 52 centers in 2002].the susceptibilities of bacteria to fluoroquinolones (fqs), especially levofloxacin, and other antimicrobial agents were investigated using 11,475 clinical isolates collected in japan during 2002. methicillin susceptible staphylococci, streptococcus pyogenes, streptococcus pneumoniae, moraxella catarrhalis, the family of enterobactericeae, haemophilus influenzae and acinetobacter spp. exhibited stable and high susceptibilities to fqs. the rate of fqs-resistant mrsa was 80 approximately 90%, bein ...200515849869
a potent sperm motility-inhibiting activity of bioflavonoids from an ethnomedicine of onge, alstonia macrophylla wall ex a. dc, leaf extract.the methanol extract (me) and the n-butanol fractions of methanolic extract of alstonia macrophylla wall ex a. dc leaves were investigated on the forward motility (fm) of mammalian (goat and human) spermatozoa. the me at 600 microg ml-1 as well as fraction b at 100 microg ml-1 concentrations showed marked inhibition of sperm fm in both goat and human species when tested by microscopic and spectrophotometric methods. approximately 60-80% of the goat spermatozoa lost their fm when treated with 600 ...200515854639
detection of paragonimus heterotremus eggs in experimentally infected cats by a polymerase chain reaction-based method.a polymerase chain reaction (pcr) procedure for the detection of paragonimus heterotremus eggs in stool samples was developed and compared with stoll's egg count method. the primers were designed on the basis of a previously constructed pph-13-specific dna probe, which produced an approximate 0.5-kb amplified product. this pcr method could detect as few as 5 eggs in 0.6 g of artificially inoculated feces of a healthy control cat or as little as 1 x 10(-4) ng of p. heterotremus genomic dna. the a ...200515856902
occurrence and molecular analysis of extended-spectrum {beta}-lactamase-producing proteus mirabilis in hong kong, 1999-2002.a study was conducted to evaluate the occurrence and characterization of extended-spectrum beta-lactamases (esbls) among blood isolates of proteus mirabilis collected over a 4 year period in hong kong.200515857942
evolution of skin lesions in proteus syndrome.proteus syndrome is a rare overgrowth disorder that is generally progressive, but the natural history of the skin lesions is not known.200515858474
bacterial infections--the cause of preterm delivery.within diagnostic procedures protocols in preterm delivery, the objective of the study was to examine bacterial causes of preterm deliveries in relation to term deliveries. the study included 106 patients delivered before term (24 to 37 weeks of gestation) and 126 patients with term delivery after 37 to 42 weeks of gestation. bacterial analysis included hospital material: cervix smear in patients before the delivery and protective and functional fetal elements.200515864934
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