| cutting edge: bcl6-interacting corepressor contributes to germinal center t follicular helper cell formation and b cell helper function. | cd4(+) germinal center (gc)-t follicular helper (tfh) cells help b cells become long-lived plasma cells and memory cells. the transcriptional repressor bcl6 plays a key role in gc-tfh formation by inhibiting the expression of genes that promote differentiation into other lineages. we determined whether bcor, a component of a polycomb repressive complex that interacts with the bcl6 btb domain, influences gc-tfh differentiation. t cell-targeted bcor deficiency led to a substantial loss of peptide: ... | 2015 | 25964495 |
| epstein barr virus-induced 3 (ebi3) together with il-12 negatively regulates t helper 17-mediated immunity to listeria monocytogenes infection. | although the protective functions by t helper 17 (th17) cytokines against extracellular bacterial and fungal infection have been well documented, their importance against intracellular bacterial infection remains unclear. here, we investigated the contribution of th17 responses to host defense against intracellular bacteria listeria monocytogenes and found that th17 cell generation was suppressed in this model. unexpectedly, mice lacking both p35 and ebi3 cleared l. monocytogenes as efficiently ... | 2013 | 24068935 |
| identification of a potent microbial lipid antigen for diverse nkt cells. | semi-invariant/type i nkt cells are a well-characterized cd1d-restricted t cell subset. the availability of potent ags and tetramers for semi-invariant/type i nkt cells allowed this population to be extensively studied and revealed their central roles in infection, autoimmunity, and tumor immunity. in contrast, diverse/type ii nkt (dnkt) cells are poorly understood because the lipid ags that they recognize are largely unknown. we sought to identify dnkt cell lipid ag(s) by interrogating a panel ... | 2015 | 26254340 |
| treatment with incomplete freund's adjuvant and listeria monocytogenes delays diabetes via an interleukin-17-secretion-independent pathway. | non-obese diabetes (nod) mice are widely used as an animal model in studies of type i diabetes (tid). treatment with complete freund's adjuvant (cfa) in pro-diabetic nod mice is known to inhibit disease progression by activating cd1d-specific natural killer (nk) t cells and inducing interleukin (il)-17 secretion in innate immune cells. the aim of the present study was to examine the effect of incomplete freund's adjuvant (ifa) and l. monocytogenes treatment on the development of tid in nod mice. ... | 2015 | 26136917 |
| normal development and function of t cells in proline rich 7 (prr7) deficient mice. | transmembrane adaptor proteins (traps) are important organisers for the transduction of immunoreceptor-mediated signals. prr7 is a trap that regulates t cell receptor (tcr) signalling and potently induces cell death when overexpressed in human jurkat t cells. whether endogenous prr7 has a similar functional role is currently unknown. to address this issue, we analysed the development and function of the immune system in prr7 knockout mice. we found that loss of prr7 partially impairs development ... | 2016 | 27657535 |
| minicircle dna is superior to plasmid dna in eliciting antigen-specific cd8+ t-cell responses. | clinical trials reveal that plasmid dna (pdna)-based gene delivery must be improved to realize its potential to treat human disease. current pdna platforms suffer from brief transgene expression, primarily due to the spread of transcriptionally repressive chromatin initially deposited on plasmid bacterial backbone sequences. minicircle (mc) dna lacks plasmid backbone sequences and correspondingly confers higher levels of sustained transgene expression upon delivery, accounting for its success in ... | 2013 | 23689601 |
| i kappa b kinase alpha (ikkα) activity is required for functional maturation of dendritic cells and acquired immunity to infection. | dendritic cells (dc) are required for priming antigen-specific t cells and acquired immunity to many important human pathogens, including mycobacteriuim tuberculosis (tb) and influenza. however, inappropriate priming of auto-reactive t cells is linked with autoimmune disease. understanding the molecular mechanisms that regulate the priming and activation of naïve t cells is critical for development of new improved vaccines and understanding the pathogenesis of autoimmune diseases. the serine/thr ... | 2013 | 23422957 |
| a one health overview, facilitating advances in comparative medicine and translational research. | a1 one health advances and successes in comparative medicine and translational researchcheryl strouda2 dendritic cell-targeted gorilla adenoviral vector for cancer vaccination for canine melanomaigor dmitriev, elena kashentseva, jeffrey n. bryan, david t. curiela3 viroimmunotherapy for malignant melanoma in the companion dog modeljeffrey n. bryan, david curiel, igor dmitriev, elena kashentseva, hans rindt, carol reinero, carolyn j. henrya4 of mice and men (and dogs!): development of a commercial ... | 0 | 27558513 |
| cloning, expression analysis, and rna interference study of a horma domain containing autophagy-related gene 13 (atg13) from the coleopteran beetle, tenebrio molitor. | autophagy is a process that is necessary during starvation, as it replenishes metabolic precursors by eliminating damaged organelles. autophagy is mediated by more than 35 autophagy-related (atg) proteins that participate in the nucleation, elongation, and curving of the autophagosome membrane. in a pursuit to address the role of autophagy during development and immune resistance of the mealworm beetle, tenebrio molitor, we screened atg gene sequences from the whole-larva transcriptome database. ... | 2015 | 26136688 |
| a listeria monocytogenes-based vaccine that secretes sand fly salivary protein ljm11 confers long-term protection against vector-transmitted leishmania major. | cutaneous leishmaniasis is a sand fly-transmitted disease characterized by skin ulcers that carry significant scarring and social stigmatization. over the past years, there has been cumulative evidence that immunity to specific sand fly salivary proteins confers a significant level of protection against leishmaniasis. in this study, we used an attenuated strain of listeria monocytogenes as a vaccine expression system for ljm11, a sand fly salivary protein identified as a good vaccine candidate. ... | 2014 | 24733091 |
| proteomic analysis of egg white heparin-binding proteins: towards the identification of natural antibacterial molecules. | the chicken egg resists most environmental microbes suggesting that it potentially contains efficient antimicrobial molecules. considering that some heparin-binding proteins in mammals are antibacterial, we investigated the presence and the antimicrobial activity of heparin-binding proteins from chicken egg white. mass spectrometry analysis of the proteins recovered after heparin-affinity chromatography, revealed 20 proteins, including known antimicrobial proteins (avidin, lysozyme, tenp, ovalbu ... | 2016 | 27294500 |
| minibactenecins chbac7.nα and chbac7. nβ - antimicrobial peptides from leukocytes of the goat capra hircus. | antimicrobial peptides (amps) of neutrophils play an important role in the animal and human host defenses. we have isolated two amps (average molecular masses of 2895.5 and 2739.3 da), with potent antimicrobial activity from neutrophils of the domestic goat (capra hircus). a structural analysis of the obtained peptides revealed that they encompass n-terminal fragments (1-21 and 1-22) of the proline-rich peptide bactenecin 7.5. the primary structure of caprine bactenecin 7.5 had been previously d ... | 0 | 27795854 |
| assessment of the microbiological quality of fresh produce on sale in sicily, italy: preliminary results. | fresh produce occupies an increasingly important place in the human food supply because of its health-promoting nutritional properties. most fresh produce is eaten raw or after minimal processing and, consequently, pathogen contamination can represent a serious health risk. there has been an increase in foodborne outbreaks and cases associated with fresh produce, but literature data about the prevalence of pathogen contamination are inconsistent. this study was undertaken to assess the hygienic ... | 2015 | 25984508 |
| acanthamoeba release compounds which promote growth of listeria monocytogenes and other bacteria. | listeria monocytogenes can grow as a saphrophyte in diverse habitats, e.g., soil, rivers, lakes, and on decaying plant material. in these environments, the bacteria are frequently exposed to predatory protozoa such as acanthamoeba. although l. monocytogenes is a facultative intracellular pathogen it does not infect or survive intracellular in acanthamoeba castellanii, unlike several other facultative intracellular bacteria. instead, motile l. monocytogenes can form large aggregates on amoebal ce ... | 2014 | 24562324 |
| acanthamoeba feature a unique backpacking strategy to trap and feed on listeria monocytogenes and other motile bacteria. | despite its prominent role as an intracellular human pathogen, listeria monocytogenes normally features a saprophytic lifestyle, and shares many environmental habitats with predatory protozoa. earlier studies claimed that acanthamoeba may act as environmental reservoirs for l. monocytogenes, whereas others failed to confirm this hypothesis. our findings support the latter and provide clear evidence that l. monocytogenes is unable to persist in acanthamoeba castellanii and a. polyphaga. instead, ... | 2013 | 22925311 |
| bacterial diversity of floor drain biofilms and drain waters in a listeria monocytogenes contaminated food processing environment. | sanitation protocols are applied on a daily basis in food processing facilities to prevent the risk of cross-contamination with spoilage organisms. floor drain water serves along with product-associated samples (slicer dust, brine or cheese smear) as an important hygiene indicator in monitoring listeria monocytogenes in food processing facilities. microbial communities of floor drains are representative for each processing area and are influenced to a large degree by food residues, liquid efflue ... | 2016 | 26881738 |
| the role of universal stress proteins in bacterial infections. | universal stress proteins are ubiquitously expressed in bacteria, archaea and plants and other eukaryotes. a general property of usps is their role in adaptation of bacteria to oxidative stress, high temperature, low ph and/or hypoxia. there is increasing evidence that these proteins facilitate the adaption of bacterial pathogens to the human host environment, thereby facilitating colonisation and pathogenicity. usps in mycobacterium tuberculosis are well studied and may play a role in latency o ... | 2017 | 28120710 |
| antimicrobial activity, ame resistance, and a-site binding studies of anthraquinone-neomycin conjugates. | the antibacterial effects of aminoglycosides are based on their association with the a-site of bacterial rrna and interference with the translational process in the bacterial cell, causing cell death. the clinical use of aminoglycosides is complicated by resistance and side effects, some of which arise from their interactions with the human mitochondrial 12s rrna and its deafness-associated mutations, c1494u and a1555g. we report a rapid assay that allows screening of aminoglycoside compounds to ... | 2017 | 28103015 |
| evaluation of the effects of ultraviolet light on bacterial contaminants inoculated into whole milk and colostrum, and on colostrum immunoglobulin g. | raw milk and colostrum can harbor dangerous microorganisms that can pose serious health risks for animals and humans. according to the usda, more than 58% of calves in the united states are fed unpasteurized milk. the aim of this study was to evaluate the effect of uv light on reduction of bacteria in milk and colostrum, and on colostrum igg. a pilot-scale uv light continuous (uvc) flow-through unit (45 j/cm(2)) was used to treat milk and colostrum. colostrum and sterile whole milk were inoculat ... | 2014 | 24582452 |
| biosynthetic enhancement of the detection of bacteria by the polymerase chain reaction. | molecular viability testing (mvt) was previously reported to specifically detect viable bacterial cells in complex samples. in mvt, brief nutritional stimulation induces viable cells, but not non-viable cells, to produce abundant amounts of species-specific ribosomal rna precursors (pre-rrna). quantitative polymerase chain reaction (qpcr) is used to quantify specific pre-rrnas in a stimulated aliquot relative to a non-stimulated control. in addition to excluding background signal from non-viable ... | 2014 | 24466092 |
| antimicrobial efficacy and safety of mucoadhesive exopolymer produced by acinetobacter haemolyticus. | this study evaluated five extracellular polymers of bacterial origin possessing mucoadhesive properties for their antimicrobial properties and toxicological characteristics. of the five tested mucoadhesive biopolymers, the extracellular polymer produced by a strain of acinetobacter haemolyticus exhibited broad antimicrobial efficacy towards yersinia enterocolitica, salmonella typhimurium, listeria monocytogenes, escherichia coli o157:h7 and bacillus subtilis. significant (p<0.05) inhibition of g ... | 2017 | 27720965 |
| phylogenetic analysis of antimicrobial lactic acid bacteria from farmed seabass dicentrarchus labrax. | the use of lactic acid bacteria (lab) in the prevention or reduction of fish diseases is receiving increasing attention. in the present study, 47 lab strains were isolated from farmed seabass ( dicentrarchus labrax ) and were phenotypically and phylogenetically analysed by 16s rdna and randomly amplified polymorphic dna - polymerase chain reaction (rapd-pcr). their antimicrobial effect was tested in vitro against a wide variety of pathogenic and spoilage bacteria. most of the strains isolated we ... | 2012 | 22439634 |
| aerobic bacterial microbiota isolated from the cloaca of the european pond turtle (emys orbicularis) in poland. | we conducted a comparative analysis of the aerobic cloacal bacteria of european pond turtles (emys orbicularis) living in their natural environment and juvenile turtles reared under controlled conditions in a breeding center. we included 130 turtles in the study. the aerobic bacteria isolated from the cloaca of the juvenile turtles were less diverse and more prevalent than the bacteria isolated from free-living adults. we isolated 17 bacterial species from juvenile captive turtles, among which t ... | 2015 | 25380369 |
| evidence of antibacterial activities in peptide fractions originating from snow crab (chionoecetes opilio) by-products. | antibacterial peptide fractions generated via proteolytic processing of snow crab by-products exhibited activity against gram-negative and gram-positive bacteria. among the bacterial strains tested, peptide fractions demonstrated inhibitory activity against the gram-negative bacteria such as aeromonas caviae, aeromonas hydrophila, campylobacter jejuni, listonella anguillarum, morganella morganii, shewanella putrefasciens, vibrio parahaemolyticus and vibrio vulnificus and against a few gram-posit ... | 2010 | 26781242 |
| molecular characterization and functional analysis of a piscidin gene in large yellow croaker (larimichthys crocea). | the piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. in this study, we cloned piscidin-5-like type 3 (lcpis5lt3) in large yellow croaker (larimichthys crocea). multiple alignments with other known piscidins revealed amino acid conservation throughout the fish, especially at the signal peptide (22 amino acids). the phylogenetic tree confirmed that lcpis5lt3 and large yellow croaker piscidin-5-l ... | 2016 | 28105799 |
| effect of acidic electrolyzed water-induced bacterial inhibition and injury in live clam (venerupis philippinarum) and mussel (mytilus edulis). | the effect of acidic electrolyzed water (aew) on inactivating escherichia coli o104:h4, listeria monocytogenes, aeromonas hydrophila, vibrio parahaemolyticus and campylobacter jejuni in laboratory contaminated live clam (venerupis philippinarum) and mussel (mytilus edulis) was investigated. the initial levels of bacterial contamination were: in clam 4.9 to 5.7log10cfu/g, and in mussel 5.1 to 5.5log10cfu/g. two types of aew were used for treatment time intervals of 1 and 2h: strong (saew) with an ... | 2016 | 27208583 |
| antimicrobial activity of neo-clerodane diterpenoids isolated from lamiaceae species against pathogenic and food spoilage microorganisms. | antimicrobial activity of nineteen neo-clerodane diterpenoids, isolated from the acetone extracts of the aerial parts of scutellaria and salvia species (lamiaceae) were tested against thirteen strains belonging to nine different species of pathogenic and food spoilage bacteria aeromonas hydrophila, bacillus cereus, escherichia coli, listeria monocytogenes, proteus vulgaris, pseudomonas aeruginosa, pseudomonas fluorescens, salmonella abony and staphylococcus aureus as well as against two yeast st ... | 2015 | 26749799 |
| molecular analysis of dominant species in listeria monocytogenes-positive biofilms in the drains of food processing facilities. | listeria monocytogenes exhibits symbiotic codependence with the dominant commensal bacteria, which may help it avoid being removed or inactivated by disinfectants in local environments. in this study, we investigated l. monocytogenes-positive biofilms at food production facilities, and the dominant bacterial species of the biofilms were identified to determine the properties of the microbiological background. for this purpose, the iso 11290 method was used for the detection and isolation of l. m ... | 2016 | 26658820 |
| the impact of carvacrol on ammonia and biogenic amine production by common foodborne pathogens. | the impact of carvacrol at different levels (0.1%, 0.5%, and 1%) on ammonia (amn) and biogenic amines (bas) production by 8 common foodborne pathogens (fbps) (staphylococcus aureus, escherichia coli, klebsiella pneumoniae, enterococcus faecalis, pseudomonas aeruginosa, listeria monocytogenes, aeromonas hydrophila, and salmonella paratyphi a) was studied using a rapid high-performance liquid chromatography method. significant differences among bacteria (p < 0.05) in amn and ba production were obs ... | 2015 | 26580308 |
| molecular characterization of a transmembrane c-type lectin receptor gene from ayu (plecoglossus altivelis) and its effect on the recognition of different bacteria by monocytes/macrophages. | c-type lectin receptors (ctlrs) play vital roles in immune responses as pattern-recognition receptors (prrs). in this study, we identified a novel c-type lectin receptor (pactlrc) gene from ayu, plecoglossus altivelis. predicted pactlrc is a single transmembrane receptor with a typical carbohydrate recognition domain (crd) at its c-terminus. sequence comparison and phylogenetic tree analysis showed that pactlrc was most closely related to atlantic salmon (salmo salar) clrc, but was significantly ... | 2015 | 26010409 |
| efficacy of ultraviolet (uv-c) light in a thin-film turbulent flow for the reduction of milkborne pathogens. | nonthermal technologies are being investigated as viable alternatives to, or supplemental utilization, with thermal pasteurization in the food-processing industry. in this study, the effect of ultraviolet (uv)-c light on the inactivation of seven milkborne pathogens (listeria monocytogenes, serratia marcescens, salmonella senftenberg, yersinia enterocolitica, aeromonas hydrophila, escherichia coli, and staphylococcus aureus) was evaluated. the pathogens were suspended in ultra-high-temperature w ... | 2015 | 25884367 |
| microbial biofilms in seafood: a food-hygiene challenge. | seafood forms a part of a healthy diet. however, seafood can be contaminated with foodborne pathogens, resulting in disease outbreaks. because people consume large amounts of seafood, such disease outbreaks are increasing worldwide. seafood contamination is largely due to the naturally occurring phenomenon of biofilm formation. the common seafood bacterial pathogens that form biofilms are vibrio spp., aeromonas hydrophila, salmonella spp., and listeria monocytogenes. as these organisms pose a gl ... | 2015 | 25846914 |
| antimicrobial efficacy and in vivo toxicity studies of a quaternized biopolymeric flocculant. | this study evaluated the antibacterial spectrum and safety of a chemically modified biopolymeric flocculant (tmb) against waterborne pathogens. the biopolymer previously characterized as polysaccharide with flocculating activity is produced extracellularly by the bacterium klebsiella terrigena. the amino sugars on the polymer were chemically modified by quaternization, which resulted in n,n,n trimethyl biopolymer (tmb). quaternization was effective in imparting biocidal activity to tmb against f ... | 2014 | 25473973 |
| the sensitivity of bacterial foodborne pathogens to croton blanchetianus baill essential oil. | the aim of this study was to assess the activity of essential oil extracted from the leaves of c. blanchetianus baill, popularly known as "marmeleiro", in inhibiting the growth and survival of pathogenic microorganisms in food by determining their survival in vitro and by observing the behaviour of listeria monocytogenes inoculated into a food model (meat cubes) that was stored at refrigeration temperature (7 ± 1 °c) for 4 days. the results indicated a bactericidal effect against aeromonas hydro ... | 2013 | 24688510 |
| synthesis and evaluation of antibacterial activity of quaternized biopolymer from klebsiella terrigena. | microbial exopolymer with antimicrobial properties, in particular, has gathered considerable interest due to their enormous scope of modification and wide gamut of application. the purpose of present study was to evaluate the antibacterial spectrum of a chemically modified biopolymeric flocculant produced by klebsiella terrigena. | 2014 | 24268027 |
| development of a quaternized chitosan with enhanced antibacterial efficacy. | the antibacterial activity of a water-soluble chitosan derivative prepared by chemical modification to quaternary ammonium compound n,n,n-trimethylchitosan (tc) was investigated against four selected waterborne pathogens: aeromonas hydrophila atcc 35654, yersinia enterocolitica atcc 9610, listeria monocytogenes atcc 19111 and escherichia coli o157:h7 atcc 32150. an inactivation of 4 log cfu/ml of all waterborne pathogens was noted for the quaternized chitosan as compared with chitosan over a sho ... | 2013 | 23981870 |
| synthesis and anti-microbial potencies of 1-(2-hydroxyethyl)-3-alkylimidazolium chloride ionic liquids: microbial viabilities at different ionic liquids concentrations. | three 1-(2-hydroxyethyl)-3-alkylimidazolium chloride room temperature ionic liquids (ils) [2ohimc(n)][cl]; (n=0, 1, 4) have been synthesized from the appropriate imidazole precursors and characterized by ir and nmr spectroscopies and elemental analysis. their anti-microbial activities were investigated using the well-diffusion method. the viabilities of escherichia coli, aeromonas hydrophila, listeria monocytogenes and salmonella enterica as a function of il concentrations were studied. the mini ... | 2013 | 23107478 |
| the influences of natural zeolite (cliptinolite) on ammonia and biogenic amine formation by foodborne pathogen. | the influence of natural zeolite on biogenic amines (bas) and ammonia (amn) production by eight common gram negative and positive foodborne pathogens (fbp) were investigated in histidine decarboxylase broth (hdb). presence of 1% zeolite in the hdb resulted in significantly higher amn production. histamine (his) production by gram positive bacteria was as low as 0.5 mg/l, whereas escherichia coli produced 18.96 mg/l of his. the use of zeolite also significantly suppressed his accumulation by e. c ... | 2012 | 22860594 |
| the function of lactic acid bacteria and brine solutions on biogenic amine formation by foodborne pathogens in trout fillets. | the influences of lactic acid bacteria and brine solutions on the biogenic amine formation by staphylococcus aureus, escherichia coli, klebsiella pneumoniae, enterococus faecalis, pseudomonas aeruginosa, listeria monocytogenes, aeromonas hydrophila and salmonella paratyphi a in fermented trout fillets were investigated. fish fillets were divided into four groups, group 1 without any lactic acid bacteria inoculation, group 2 and group 3 with different salt concentration inoculated with lactic aci ... | 2011 | 25212358 |
| synergistic and antagonistic effect of lactic acid bacteria on tyramine production by food-borne pathogenic bacteria in tyrosine decarboxylase broth. | the effect of lactic acid bacteria (lab) strains on tyramine (tyr) and also other biogenic amines (ba) production by eight common food-borne pathogen (fbp) in tyrosine decarboxylase broth (tdb) was investigated by using a rapid hplc method. significant differences were observed among the fbp strains in ammonia (amn) and ba production apart from tryptamine, histamine (his) and spermine formation (p<0.05). salmonella paratyphi a was characterised as the main amine producer. lab had an important sy ... | 2011 | 25214109 |
| survival of aeromonas hydrophila and listeria monocytogenes on fresh vegetables stored under moderate vacuum. | storage at 6.5°c under moderate vacuum effectively prevented growth of aeromonas hydrophila on chicory endive, but had only a limited inhibitory effect on the growth of the organism on mung bean sprouts. growth of listeria monocytogenes on chicory endive was strongly stimulated under these conditions, whereas it was decreased on mung-bean sprouts. | 1994 | 24421192 |
| sumoylation as an integral mechanism in bacterial infection and disease progression. | post translational modification pathways regulate fundamental processes of cells and thus govern vital functions. among these, particularly the modification with small ubiquitin-like modifiers (sumo) is being recognized as a pathway crucial for cell homeostasis and health. understandably, bacterial pathogens intervene with the sumo pathway of the host for ensuring successful infection. among the bacterial pathogens known to target host sumoylation varied points of intervention are utilized. majo ... | 2017 | 28197924 |
| lessons from anaplasma phagocytophilum: chromatin remodeling by bacterial effectors. | bacterial pathogens can alter global host gene expression via histone modifications and chromatin remodeling in order to subvert host responses, including those involved with innate immunity, allowing for bacterial survival. shigella flexneri, listeria monocytogenes, chlamydia trachomatis, and anaplasma phagocytophilum express effector proteins that modify host histones and chromatin structure. a. phagocytophilum modulates granulocyte respiratory burst in part by dampening transcription of sever ... | 2012 | 23082961 |
| arcanobacterium haemolyticum osteomyelitis and sepsis: a diagnostic conundrum. | arcanobacterium haemolyticum can cause severe systemic infections and sepsis. thus, accurate and timely identification of the organism is essential. | 2013 | 23581632 |
| hygienisation and nutrient conservation of sewage sludge or cattle manure by lactic acid fermentation. | manure from animal farms and sewage sludge contain pathogens and opportunistic organisms in various concentrations depending on the health of the herds and human sources. other than for the presence of pathogens, these waste substances are excellent nutrient sources and constitute a preferred organic fertilizer. however, because of the pathogens, the risks of infection of animals or humans increase with the indiscriminate use of manure, especially liquid manure or sludge, for agriculture. this p ... | 2015 | 25786255 |
| biological activity of the essential oils from cinnamodendron dinisii and siparuna guianensis. | this study had analyzed the antibacterial, antifungal and trypanocidal activity of the essential oils from cinnamodendron dinisii schwacke (canellaceae) and siparuna guianensis aublet (siparunaceae). the essential oils were obtained from fresh leaves by hydrodistillation, using a modified clevenger apparatus. chemical analysis by gas-liquid chromatography coupled to mass spectrometry (gc-ms) showed that these essential oils are rich in monoterpene and sesquiterpene hydrocarbons. activity against ... | 2015 | 26221107 |
| flexible thin-layer plasma inactivation of bacteria and mold survival in beef jerky packaging and its effects on the meat's physicochemical properties. | the aims of the present study were to examine the use of a flexible thin-layer plasma system in inactivating bacteria and mold on beef jerky in a commercial package and to evaluate the physicochemical changes of the jerky. after plasma treatment for 10min, escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium, and aspergillus flavus populations on the beef jerky were reduced by approximately 2 to 3log cfu/g. no significant changes in metmyoglobin content, shear force, and myof ... | 2017 | 27718408 |
| screening and characterization of potential bacillus starter cultures for fermenting low-salt soybean paste (doenjang). | the bacterial strains were screened as potential starters for fermenting low-salt doenjang (a korean traditional fermented soybean paste) using korean doenjang based on proteolytic and antipathogenic activities under 6.5-7.5% nacl conditions. phylogenetic analysis based on 16s rrna gene sequences showed that they all belonged to the genus bacillus. proteolytic and antipathogenic activities against escherichia coli, bacillus cereus, staphylococcus aureus, listeria monocytogenes, and aspergillus f ... | 2016 | 26718466 |
| design, structural and spectroscopic elucidation, and the in vitro biological activities of new diorganotin dithiocarbamates. | the reaction of 2,2-dimethoxy-n-methylethyllamine or 2-methyl-1,3-dioxolane with cs(2) in alkaline media produced two novel dithiocarbamate salts. subsequent reactions with organotin halides yielded six new complexes: [snme(2){s(2)cnr(r(1))(2)}(2)] (1), [sn(n-bu)(2){s(2)cnr(r(1))(2)}(2)] (2), [snph(2){s(2)cnr(r(1))(2)}(2)] (3), [snme(2){s(2)cnr(r(2))(2)}(2)] (4), [sn(n-bu)(2){s(2)cnr(r(2))(2)}(2)] (5), [snph(2){s(2)cnr(r(2))(2)}(2)] (6), where r = methyl, r(1) = ch(2)ch(ome)(2), and r(2) = 2-met ... | 2012 | 23159807 |
| differential roles of caspase-1 and caspase-11 in infection and inflammation. | caspase-1, also known as interleukin-1β (il-1β)-converting enzyme (ice), regulates antimicrobial host defense, tissue repair, tumorigenesis, metabolism and membrane biogenesis. on activation within an inflammasome complex, caspase-1 induces pyroptosis and converts pro-il-1β and pro-il-18 into their biologically active forms. "ice(-/-)" or "casp1(-/-)" mice generated using 129 embryonic stem cells carry a 129-associated inactivating passenger mutation on the caspase-11 locus, essentially making t ... | 2017 | 28345580 |
| a study of the potential release of bioaerosols from containers as a result of reduced frequency residual waste collections. | microorganisms have the potential to grow within waste containers if waste is stored for longer periods as a result of an extended residual waste collection cycle. release of microorganisms as bioaerosols during waste collection and processing may be an occupational risk to workers within the industry. there may be many constituents of the bioaerosol that may be of concern, however, there are currently only workplace exposure limits proposed for endotoxin (90eum(-3)). a field-scale trial was est ... | 2017 | 27810739 |
| antimicrobial activity of the pygidial gland secretion of three ground beetle species (insecta: coleoptera: carabidae). | the antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, carabus ullrichii, c. coriaceus, and abax parallelepipedus, have been tested. microdilution method was applied for detection of minimal inhibitory concentrations (mics), minimal bactericidal concentrations (mbcs), and minimal fungicidal concentrations (mfcs). additionally, morpho-histology of the pygidial glands is investigated. we have tested 16 laboratory and clinical strain ... | 2016 | 27000261 |
| czech ethanol-free propolis extract displays inhibitory activity against a broad spectrum of bacterial and fungal pathogens. | propolis acts primarily as a biocide against invasive bacteria and fungi in the hive, suggesting its potential for industrial applications. in food application, propolis is considered as a chemical preservative in meat products, extending shelf life of frozen meat and other food. the mechanism of action is still unclear due to the synergy of multiple compounds contained in propolis and due to parallel targeting of multiple pathways within each affected organism. here, we examined the antimicrobi ... | 2013 | 23915150 |
| isolation and characterisation of an enterocin p-producing enterococcus lactis strain from a fresh shrimp (penaeus vannamei). | screening for lactic acid bacteria (lab) from fresh shrimp samples (penaeus vannamei) collected from retail seafood markets in the tunisian's coast, resulted in the isolation of an enterococcus strain termed q1. this strain was selected for its antagonistic activity against pathogenic bacteria such as listeria monocytogenes, pseudomonas aeruginosa, lactococcus garvieae and against fungi (aspergillus niger and fusarium equiseti). the q1 strain was characterised using standard morphological and bi ... | 2017 | 28265787 |
| extracts containing clps of bacillus amyloliquefaciens jn68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant staphylococcus aureus and listeria monocytogenes. | bacillus amyloliquefaciens jn68, which has been discussed with regards to its antimicrobial activities, was successfully isolated from healthy chicken intestines in the present study. using the spot-on-the-lawn antagonism method, the preliminary study indicated that a suspension culture of the b. amyloliquefaciens jn68 strain can inhibit the growth of aspergillus niger and penicillium pinophilum. furthermore, the cyclic lipopeptides (clps) produced by the b. amyloliquefaciens jn68 strain were fu ... | 2016 | 27840979 |
| active edible coating and γ-irradiation as cold combined treatments to assure the safety of broccoli florets (brassica oleracea l.). | the antimicrobial activity of essential oils (eos), organic acid (oa) salts and natamycin, a natural antifungal produced during fermentation by the bacterium streptomyces natalensis, was assessed against four pathogens (escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium and aspergillus niger). the minimum inhibitory concentration (mic) of each antimicrobial (am) was assessed to determine their efficiency on tested microbial species in order to select the most efficient. the ... | 2017 | 27744210 |
| mechanistic aspects of biologically synthesized silver nanoparticles against food- and water-borne microbes. | in the present study, silver nanoparticles (agnps) synthesized from aqueous leaves extract of malva crispa and their mode of interaction with food- and water-borne microbes were investigated. formation of agnps was conformed through uv-vis, fe-sem, eds, afm, and hr-tem analyses. further the concentration of silver (ag) in the reaction mixture was conformed through icp-ms analysis. different concentration of nanoparticles (1-3 mm) tested to know the inhibitory effect of bacterial pathogens such a ... | 2015 | 26178241 |
| composition, antimicrobial, antioxidant, and antiproliferative activity of origanum dictamnus (dittany) essential oil. | nowadays, there has been an increased interest in essential oils from various plant origins as potential antimicrobial, antioxidant, and antiproliferative agents. this trend can be mainly attributed to the rising number and severity of food poisoning outbreaks worldwide along with the recent negative consumer perception against artificial food additives and the demand for novel functional foods with possible health benefits. origanum dictamnus (dittany) is an aromatic, tender perennial plant tha ... | 2015 | 25952773 |
| purification of a novel chitin-binding lectin with antimicrobial and antibiofilm activities from a bangladeshi cultivar of potato (solanum tuberosum). | a new chitin-binding lectin was purified from a bangladeshi cultivar 'deshi' of potato (solanum tuberosum l.) through anion-exchange and affinity chromatographies using a chitin column. sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) showed the molecular mass of the lectin as 20,000 daltons. this molecular mass was almost half of the molecular masses of chitin-binding lectins derived from other potatoes. the lectin showed both bactericidal and growth-inhibiting activities a ... | 2014 | 24980018 |
| [determination of the antimicrobial capacity of green tea (camellia sinensis) against the potentially pathogenic microorganisms escherichia coli, salmonella enterica, staphylococcus aureus, listeria monocytogenes, candida albicans and aspergillus niger]. | many studies can be found in scientific literature demonstrating the antimicrobial capacity of different herbs, including green tea. never-theless, many results are divergent or cannot be compared. several green tea formulations may be found in market, but there is scarce or non-information about its activity. in this work, the potential antimicrobial effect of 50 samples of dry green tea and in 10% infusion against escherichia coli, salmonella enterica, listeria monocytogenes, staphylococcus au ... | 2013 | 25362825 |
| in vitro antimicrobial activity of pistachio (pistacia vera l.) polyphenols. | we investigated the antimicrobial properties of polyphenol-rich fractions derived from raw shelled and roasted salted pistachios. american type culture collection (atcc), food and clinical isolates, of gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, pseudomonas mirabilis), gram-positive bacteria (listeria monocytogenes, enterococcus hirae, enterococcus faecium, bacillus subtilis, staphylococcus epidermidis, staphylococcus aureus), the yeasts candida albicans and candida parapsi ... | 2013 | 23350629 |
| fermentative recovery of lipids and proteins from freshwater fish head waste with reference to antimicrobial and antioxidant properties of protein hydrolysate. | effectiveness of fermentation using lactic acid bacteria (lab) for recovering lipids and proteins simultaneously from freshwater fish head (fwh) was evaluated. three different proteolytic lab (pediococcus acidilactici ncim5368, enterococcus faecium ncim5335 and pediococcus acidilactici fd3) isolated from fish processing wastes were employed in the fermentation process. the fermentation conditions involved 10 % (w/w) glucose, 2 % (w/w) nacl and 10 % (v/w) lab cultures at 37 °c. the process result ... | 2014 | 25190843 |
| bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides. | several products of industrial interest are produced by bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. this study aimed to investigate the potential of two bacterial isolates (p5 and c3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. phylogenetic analyses showed close relation of isolates p5 and c3 with bacillus amyloliquefaciens and bacillus thuringie ... | 2017 | 28197131 |
| isolation and characterization of a broad spectrum bacteriocin from bacillus amyloliquefaciens rx7. | we isolated a bacillus strain, rx7, with inhibitory activity against listeria monocytogenes from soil and identified it as bacillus amyloliquefaciens based on 16s rrna gene sequencing. the inhibitory activity was stable over a wide range of ph and was fully retained after 30 min at 80°c, after which it decreased gradually at higher temperatures. the activity was sensitive to the proteolytic action of α-chymotrypsin, proteinase-k, and trypsin, indicating its proteinaceous nature. this bacteriocin ... | 2016 | 27239477 |
| cyclic dipeptide cyclo(l-leucyl-l-prolyl) from marine bacillus amyloliquefaciens mitigates biofilm formation and virulence in listeria monocytogenes. | this study was intentionally focused on cyclo(l-leucyl-l-prolyl) (clp), a cyclic dipeptide with myriad pharmaceutical significance, to explore its antivirulence efficacy against the predominant foodborne pathogen,listeria monocytogenes(lm). minimum inhibitory concentration (mic) of clp against lm atcc 19111 was found to be 512 μg ml(-1) clp at sub-mics (64 128, 256 μg ml(-1)) demonstrated a profound non-bactericidal dose-dependent antibiofilm efficacy (on polystyrene and glass) against lm, which ... | 2016 | 26945590 |
| comparison of three bacillus amyloliquefaciens strains growth behaviour and evaluation of the spoilage risk during bread shelf-life. | this study aims at the characterisation of growth behaviour of three strains of bacillus amyloliquefaciens, isolated from ropy bread (atcc8473), wheat grain (ispa-s109.3) and semolina (ispa-n9.1) to estimate rope spoilage risk in pan bread during shelf-life using the sym'previus tool. cardinal values and growth/no growth boundaries were determined in broth, while artificial spore inoculations were performed in dough for various pan bread recipes to compare experimental counts with in silico grow ... | 2015 | 25481056 |
| immobilization of proteases on chitosan for the development of films with anti-biofilm properties. | bacterial resistance due to biofilm formation-particularly staphylococci biofilms-is associated with multiple problems in medical settings where biofilms can colonize medical indwelling devices and cause nosocomial infections. it was against this backdrop that we explored the anti-biofilm activities of a set of proteases against biofilm formation by staphylococcus aureus, listeria monocytogenes and pseudomonas aeruginosa. the selected screened enzymes were immobilized on chitosan to obtain films ... | 2015 | 25451753 |
| characterization of amylolysin, a novel lantibiotic from bacillus amyloliquefaciens ga1. | lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. they are capable to inhibit the growth of gram-positive bacteria, including listeria monocytogenes, staphylococcus aureus or bacillus cereus, the causative agents of food-borne diseases or nosocomial infections. lantibiotic biosynthetic machinery is encoded by gene cluster composed by a structural gene that codes for a pre-lantibiotic peptide and other genes involved in ... | 2013 | 24349428 |
| dual mode of action of amylolysin: a type-b lantibiotic produced by bacillus amyloliquefaciens ga1. | the partial genome sequencing of bacillus amyloliquefaciens ga1 led to the identification of the aml gene cluster involved in the synthesis of the novel lantibiotic named amylolysin. pure amylolysin was shown to have an antibacterial activity toward gram-positive bacteria including methicillin resistant staphylococcus aureus. the lantibiotic was also found efficient to inhibit the growth of listeria monocytogenes strains on poultry meat upon a long storage at 4°c. in silico analyses of the aml g ... | 2014 | 24164268 |
| antibacterial activity of subtilosin alone and combined with curcumin, poly-lysine and zinc lactate against listeria monocytogenes strains. | in this paper, the antibacterial effects of the bacillus amyloliquefaciens-produced bacteriocin subtilosin, both alone and in combination with curcumin, ε-poly-l-lysine (poly-lysine), or zinc lactate, were examined against listeria monocytogenes. results indicated that subtilosin inhibits both of the studied bacterial strains, scott a (wild-type, nisin sensitive) and nr30 (nisin resistant). however, l. monocytogenes scott a was more sensitive to subtilosin and pure curcumin. in addition, subtilo ... | 2010 | 26781320 |
| antilisterial activity on poultry meat of amylolysin, a bacteriocin from bacillus amyloliquefaciens ga1. | this paper describes the production, the purification and the antilisterial activity of amylolysin, a novel bacteriocin from b. amyloliquefaciens ga1. the strain genome was first analysed using pcr techniques for the presence of gene clusters that direct the synthesis of characterised bacteriocins from b. amyloliquefaciens and the closely related b. subtilis. our results suggest that amylolysin corresponds to a novel bacteriocin. the effect of amylolysin on the growth of different isolates of li ... | 2010 | 26781121 |
| a self-quenching-resistant carbon nanodot powder with multicolored solid-state fluorescence for ultra-fast staining of various representative bacterial species within one minute. | in this study, we prepared self-quenching-resistant solid-state fluorescent carbon nanodots (sfcds) without any other solid matrices. the sfcds were prepared using a one-step microwave synthesis method through precise control of the heating power and time. the resulting sfcd powder showed excitation-dependent emission behavior with a maximum fluorescence quantum yield of 40%. the multicolored sfcds were successfully used as fluorescent agents for rapid staining of 14 representative bacterial spe ... | 2016 | 27874136 |
| tid: standalone software for mining putative drug targets from bacterial proteome. | tid is a standalone application, which relies on basic assumption that a protein must be essential for pathogens survival and non-homologous with host to qualify as putative target. with an input bacterial proteome, tid removes paralogous proteins, picks essential ones, and excludes proteins homologous with host organisms. the targets illustrate non-homology with at least 40 out of 84 gut microbes, considered safe for human. tid classifies proposed targets as known, novel and virulent. users can ... | 2017 | 27856224 |
| fapr: from control of membrane lipid homeostasis to a biotechnological tool. | phospholipids and fatty acids are not only one of the major components of cell membranes but also important metabolic intermediates in bacteria. since the fatty acid biosynthetic pathway is essential and energetically expensive, organisms have developed a diversity of homeostatic mechanisms to fine-tune the concentration of lipids at particular levels. fapr is the first global regulator of lipid synthesis discovered in bacteria and is largely conserved in gram-positive organisms including import ... | 2016 | 27766255 |
| recent developments in understanding the iron acquisition strategies of gram positive pathogens. | iron is a versatile redox-active catalyst and a required cofactor within a diverse array of biological processes. to almost all organisms, iron is both essential and potentially toxic, where homeostatic concentrations must be stringently maintained. within the iron-restricted host, the survival and proliferation of microbial invaders is contingent upon exploiting the host iron pool. bacteria express a multitude of complex, and often redundant means of acquiring iron, including surface-associated ... | 2015 | 25862688 |
| photocatalytic and biocidal activities of novel coating systems of mesoporous and dense tio₂-anatase containing silver nanoparticles. | here we describe the development of novel nanostructured coating systems with improved photocatalytic and antibacterial activities. these systems comprise a layer of sio2 followed by a layer of mesoporous or dense tio2-anatase, and doping with silver nanoparticles (ag nps). the coatings were synthesized via a sol-gel technique by combining colloidal ag nps with tio2 and sio2 sols. the photocatalytic activity was studied through methyl orange decomposition under uv light. results showed a great i ... | 2014 | 25175258 |
| antimicrobial light-activated materials: towards application for food and environmental safety. | to produce light-activated antimicrobial materials composed of the photodynamic dye phloxine b incorporated into paper or cellulose membranes and to investigate ability of these materials to decrease bacterial loads on their surfaces as well as on food surfaces that were in contact with these materials under illumination with regular white light. | 2014 | 25139147 |
| architecture and assembly of the gram-positive cell wall. | the bacterial cell wall is a mesh polymer of peptidoglycan--linear glycan strands cross-linked by flexible peptides--that determines cell shape and provides physical protection. while the glycan strands in thin 'gram-negative' peptidoglycan are known to run circumferentially around the cell, the architecture of the thicker 'gram-positive' form remains unclear. using electron cryotomography, here we show that bacillus subtilis peptidoglycan is a uniformly dense layer with a textured surface. we f ... | 2013 | 23600697 |
| [type i interferon and bacterial infection]. | interferons (ifns) are cytokines playing an important role in immune responses. interferons are classified into two distinct types according to specific interferon receptors(ifnr). type i ifns include ifn-α and ifn-β, whereas ifn-γ is type ii ifn. it is well known that type i ifns have important roles in the host defense against viruses through activation of interferon receptor a (ifnar). however, many recent studies have also demonstrated that type i ifns have effects on immune responses to bac ... | 2012 | 22927085 |
| hiv-1 integrase inhibitor-inspired antibacterials targeting isoprenoid biosynthesis. | we report the discovery of antibacterial leads, keto- and diketo-acids, targeting two prenyl transferases: undecaprenyl diphosphate synthase (upps) and dehydrosqualene synthase (crtm). the leads were suggested by the observation that keto- and diketo-acids bind to the active site mg(2+)/asp domain in hiv-1 integrase, and similar domains are present in prenyl transferases. we report the x-ray crystallographic structures of one diketo-acid and one keto-acid bound to crtm, which supports the mg(2+) ... | 2012 | 22662288 |
| finding the smoking gun: protein tyrosine phosphatases as tools and targets of unicellular microorganisms and viruses. | protein tyrosine phosphatases (ptps) are increasingly recognized as important effectors of host-pathogen interactions. since guan and dixon reported in 1990 that phosphatase yoph serves as an essential virulence determinant of yersinia, the field shifted significantly forward, and dozens of ptps were identified in various microorganisms and even in viruses. the discovery of extensive tyrosine signaling networks in non-metazoan organisms refuted the moth-eaten paradigm claiming that these organis ... | 2012 | 22360485 |
| bacteria and their toxins tamed for immunotherapy. | bacterial toxins share the ability to enter host cells to target various intracellular proteins and to modulate host immune responses. over the last 20 years, toxins and their mutated variants, as well as live attenuated bacteria, have been exploited for vaccination and immunotherapy of various infectious, malignant and autoimmune diseases. the ability of bordetella pertussis adenylate cyclase toxin to translocate its adenylate cyclase domain across the host cell membrane, as well as the pathway ... | 2012 | 22339216 |
| cytotoxicity, bactericidal, and antioxidant activity of sodium alginate hydrosols treated with direct electric current. | the aim of the study was to investigate the effect of using direct electric current (dc) of 0, 200, and 400 ma for five minutes on the physiochemical properties, cytotoxicity, antibacterial, and antioxidant activity of sodium alginate hydrosols with different sodium chloride concentrations. the ph, oxidation-reduction potential (orp), electrical conductivity (ec), and available chlorine concentration (acc) were measured. the effect of sodium alginate hydrosols treated with dc on staphylococcus a ... | 2017 | 28327520 |
| biofilm inhibition formation of clinical strains of pseudomonas aeruginosa mutans, photocatalytic activity of azo dye and gc-ms analysis of leaves of lagerstroemia speciosa. | the investigation was conducted to analyse the bioactive compounds from the leaf extracts of l. speciosa by gc-ms. the extracts were screened for antibacterial and antibiofilm activities against potential clinical strains. the bioactive compounds from the leaves of l. speciosa were extracted by soxhlet continuous extraction method and their chemical composition was analysed by gas chromatography-mass spectroscopy (gc-ms). the antibacterial activity was evaluated against clinical strain like stap ... | 2017 | 28319869 |
| antibacterial activity and synergistic antibacterial potential of biosynthesized silver nanoparticles against foodborne pathogenic bacteria along with its anticandidal and antioxidant effects. | silver nanoparticles plays a vital role in the development of new antimicrobial substances against a number of pathogenic microorganisms. these nanoparticles due to their smaller size could be very effective as they can improve the antibacterial activity through lysis of bacterial cell wall. green synthesis of metal nanoparticles using various plants and plant products has recently been successfully accomplished. however, few studies have investigated the use of industrial waste materials in nan ... | 2017 | 28261161 |
| an assessment of the microbiological quality of liver-based pâté in england 2012-13: comparison of samples collected at retail and from catering businesses. | the purpose of this study was to investigate the microbiological quality of liver pâté. during 2012-13, a total of 870 samples, unrelated to the investigation of food-poisoning outbreaks, were collected either at retail (46%), catering (53%) or the point of manufacture (1%) and were tested using standard methods to detect salmonella spp. or campylobacter spp., and to enumerate for listeria spp., including listeria monocytogenes, clostridium perfringens, coagulase-positive staphylococci including ... | 2017 | 28190406 |
| antimicrobial activity of kefir against various food pathogens and spoilage bacteria. | kefir is a unique fermented dairy product produced by a mixture of lactic acid bacteria, acetic acid bacteria, and yeast. here, we compared the antimicrobial spectra of four types of kefirs (a, l, m, and s) fermented for 24, 36, 48, or 72 h against eight food-borne pathogens. bacillus cereus, staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, escherichia coli, salmonella enteritidis, pseudomonas aeruginosa, and cronobacter sakazakii were used as test strains, and antibacterial ... | 2016 | 28115890 |
| antibiotic discovery throughout the small world initiative: a molecular strategy to identify biosynthetic gene clusters involved in antagonistic activity. | the emergence of bacterial pathogens resistant to all known antibiotics is a global health crisis. adding to this problem is that major pharmaceutical companies have shifted away from antibiotic discovery due to low profitability. as a result, the pipeline of new antibiotics is essentially dry and many bacteria now resist the effects of most commonly used drugs. to address this global health concern, citizen science through the small world initiative (swi) was formed in 2012. as part of swi, stu ... | 2017 | 28110506 |
| risk assessment in the recovery of food for social solidarity purposes: preliminary data. | the most recent study, conducted by politecnico of milan, on food surplus management in italy shows that in the italian food supply chain the food surplus is around 5.5 million tons/year, and the amount of food wasted is around 5.1 million tons/year. during 2015, the charitable organizations (cos) belonging to the italian food bank network, active in recovering and distributing food for social solidarity's purposes, reused 381,345 tons of food from 2292 donors. the main supplying sources of the ... | 2016 | 28058252 |
| inactivation and changes in metabolic profile of selected foodborne bacteria by 460 nm led illumination. | the objective of this study was to investigate the effect of 460 nm light-emitting diode (led) on the inactivation of foodborne bacteria. additionally, the change in the endogenous metabolic profile of led illuminated cells was analyzed to understand the bacterial response to the led illumination. six different species of bacteria (bacillus cereus, listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, pseudomonas aeruginosa and salmonella typhimurium) were illuminated with 460 ... | 2017 | 28040158 |
| antibacterial, antioxidant and anti-proliferative properties and zinc content of five south portugal herbs. | crataegus monogyna l. (rosaceae) (cm), equisetum telmateia l. (equisataceae) (et), geranium purpureum vil. (geraniaceae) (gp), mentha suaveolens ehrh. (lamiaceae) (ms), and lavandula stoechas l. spp. luisieri (lamiaceae) (ls) are all medicinal. | 2017 | 27925492 |
| differences in chemical, physical and microbiological characteristics of italian burrata cheeses made in artisanal and industrial plants of apulia region. | the burrata cheese is a traditional product from southern italy, consisting of an envelope of pasta filata (stretched curd) filled with cream and pasta filata strips (usually leftovers from mozzarella production). physical [water activity (aw), ph], chemical (moisture, nacl content) and microbiological [total viable count (tvc), listeria monocytogenes, salmonella spp., yersinia enterocolitica, bacillus cereus, escherichia coli, enterobacteriaceae, coagulase-positive staphylococci] characteristic ... | 2016 | 27853715 |
| evolution of the microbiological profile of vacuum-packed ricotta salata cheese during shelf-life. | ricotta salata cheese is a salted variety of ricotta traditionally made in sardinia (italy) from the whey remaining after the production of pecorino romano protected designation of origin or other sheep milk cheeses. ricotta salata cheese is very critical for the possible growth of pathogenic and spoilage microorganisms. sporadic cases of listeriosis associated with ricotta salata cheese have been reported over recent years. the objective of the present study was to assess the evolution of spoil ... | 2016 | 27800440 |
| raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets. | microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ... | 2017 | 27747902 |
| the comparing of antimicrobial activity of csn1s2 protein of fresh milk and yoghurt goat breed ethawah inhibited the pathogenic bacteria. | goat milk is reported to have antimicrobial activity of several pathogen bacteria that contained on food materials. the research related with antimicrobial activity of alpha-s2 casein from goat milk is relatively less than other casein components. herein, we reported the antimicrobial activity of caprine alpha-s2 casein (csn1s2) protein from ethawah breed goat milk and yoghurt in gram positive (listeria monocytogenes, staphylococcus aureus and bacillus cereus) and negative pathogen bacteria (esc ... | 2016 | 27698594 |
| simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods. | capillary electrophoresis-single strand conformation polymorphism (ce-sscp) coupled with stuffer-free multiplex ligation-dependent probe amplification (mlpa) was developed to identify 13 species of foodborne pathogens simultaneously. species-specific mlpa probes were designed for nine of these species. these probes were targeted to the groel, glya, mms, tuf, inv, ipah, nuc, vvh, and 16s rrna genes, which corresponded to bacillus cereus, campylobacter coli, cronobacter sakazakii, enterococcus spp ... | 2016 | 27494415 |
| bacteriocinogenic bacteria isolated from raw goat milk and goat cheese produced in the center of méxico. | currently, there are few reports on the isolation of microorganisms from goat milk and goat cheese that have antibacterial activity. in particular, there are no reports on the isolation of microorganisms with antibacterial activity from these products in central mexico. our objective was to isolate bacteria, from goat products, that synthesized antimicrobial peptides with activity against a variety of clinically significant bacteria. we isolated and identified lactobacillus rhamnosus, l. plantar ... | 2016 | 27407294 |
| biological activities of aerial parts extracts of euphorbia characias. | the aim of the present study was to evaluate antioxidant, antimicrobial, anti-hiv, and cholinesterase inhibitory activities of aqueous and alcoholic extracts from leaves, stems, and flowers of euphorbia characias. the extracts showed a high antioxidant activity and were a good source of total polyphenols and flavonoids. ethanolic extracts from leaves and flowers displayed the highest inhibitory activity against acetylcholinesterase and butyrylcholinesterase, showing potential properties against ... | 2016 | 27314007 |
| a rapid multiplex real-time pcr high-resolution melt curve assay for the simultaneous detection of bacillus cereus, listeria monocytogenes, and staphylococcus aureus in food. | three important foodborne pathogens, bacillus cereus, listeria monocytogenes, and staphylococcus aureus, are of great concern for food safety. they may also coexist in food matrices and, in the case of b. cereus and s. aureus, the resulting illnesses can resemble each other owing to similar symptoms. therefore, their simultaneous detection may have advantages in terms of cost savings and rapidity. given this context, a rapid multiplex real-time pcr high-resolution melt curve assay for the simult ... | 2016 | 27296430 |
| assessment of the microbiological quality of meat pies from retail sale in england 2013. | outbreaks of foodborne illness caused by bacillus cereus and listeria monocytogenes in england associated with meat pie consumption were detected in 2012. to obtain baseline data for pies unrelated to outbreaks, 862 samples of ready-to-eat meat pies were collected at retail or from catering facilities in england in 2013 and examined to enumerate food-poisoning bacteria and indicator organisms using organization for standardization (iso) methods for listeria spp. including l. monocytogenes (iso 1 ... | 2016 | 27296425 |