| restriction maps of five autographa californica mnpv variants, trichoplusia ni mnpv, and galleria mellonella mnpv dnas with endonucleases smai, kpni, bamhi, saci, xhoi, and ecori. | the restriction sites of autographa californica nuclear polyhedrosis virus (acmnpv) e2 dna were mapped for the endonucleases smai, kpni, bamhi, saci, xhoi, and ecori. the restriction maps of four other acmnpv variants, trichoplusia ni (tnmnpv), and galleria mellonella (gmmnpv) genomes were determined and compared to the endonuclease cleavage maps of acmnpv e2 dna. the viral structural polypeptides of acmnpv variants s3, e2, s1, m3, and r9 were the same when analyzed by polyacrylamide gel electro ... | 1979 | 16789179 |
| genetic analysis of a baculovirus, autographa californica nuclear polyhedrosis virus i. isolation of temperature-sensitive mutants and assortment into complementation groups. | temperature-sensitive (ts) mutants were isolated from the baculovirus autographa californica (alfalfa looper) mnpv, grown in spodoptera frugiperda (fall armyworm) cells in the presence of n-methyl-n'-nitro-n-nitrosoguanidine. of 567 plaque isolates screened, 27 were temperature sensitive (ts), representing a mutation frequency of 4.8%. ten ts mutants were studied in detail: six failed to yield nonoccluded virus at 33 degrees c (nov mutants), whereas the other four produced nonoccluded virus but ... | 1979 | 16789180 |
| isolation, complementation, and initial characterization of temperature-sensitive mutants of the baculovirus autographa californica nuclear polyhedrosis virus. | sixteen temperature-sensitive mutants of autographa californica nuclear polyhedrosis virus were isolated. several interesting phenotypes were observed. a large proportion of the mutants were unable to form polyhedral occlusion bodies (polyhedra) at the nonpermissive temperature (32.5 degrees c). at 32.5 degrees c, one mutant formed plaques in which the cells lacked polyhedra. another mutant type was defective in the production of progeny extracellular nonoccluded virus and produced a "plaque" co ... | 1979 | 16789181 |
| restriction map of rachiplusia ou and rachiplusia ou-autographa californica baculovirus recombinants. | the restriction sites of rachiplusia ou nuclear polyhedrosis virus (romnpv) dna were mapped for the endonucleases smai, kpni, bamhi, saci, xhoi, and ecori. of the 60 dna restriction sites of romnpv, 35 mapped in similar positions as compared to the restriction sites of autographa californica nuclear polyhedrosis virus (acmnpv) dna. two plaque-purified viruses, obtained from randomly picked plaques of a wild-type isolate of romnpv, were recombinants of romnpv and acmnpv. the recombinants were sho ... | 1980 | 16789188 |
| comparison of the structure of c- and n-polyhedrins from two occluded viruses pathogenic for orgyia pseudotsugata. | c-and n-polyhedrins from a cytoplasmic polyhedrosis virus (a double-stranded rna virus) and a nuclear polyhedrosis virus (a dna virus), respectively, of orgyia pseudotsugata were compared. although both polyhedrins appear to stabilize their respective virions and have similar molecular weights, they differed in amino acid composition, tryptic peptide elution profiles from a cation-exchange resin, and n-terminal amino acid sequence and showed no antigenic relatedness. this suggests that these two ... | 1980 | 16789195 |
| physical maps of autographa californica and rachiplusia ou nuclear polyhedrosis virus recombinants. | tn-368 cells were infected simultaneously with the closely related autographa california (acmnpv) and rachiplusia ou (romnpv) nuclear polyhedrosis viruses. progeny viral isolates were plaque purified, and their dnas were analyzed with restriction endonucleases. of 100 randomly cloned plaques, 7 were acmnpv and romnpv recombinants, 5 were romnpv, and 88 were acmnpv. the recombinants contained dna sequences derived from both parental genomes. by comparing the restriction cleavage patterns of paren ... | 1980 | 16789198 |
| alpha-amanitin-resistant viral rna synthesis in nuclei isolated from nuclear polyhedrosis virus-infected heliothis zea larvae and spodoptera frugiperda cells. | [(3)h]rna was synthesized in nuclei isolated at various times postinfection from the fat bodies of heliothis zea larvae infected with h. zea nuclear polyhedrosis virus and from cultured spodoptera frugiperda cells infected with autographa californica nuclear polyhedrosis virus. to detect virus-specific rna synthesis, the [(3)h]rna was hybridized to denatured viral dna immobilized on nitrocellulose filters. nuclear polyhedrosis virus-specific rna synthesis in the infected nuclei isolated from h. ... | 1981 | 16789208 |
| spodoptera frugiperda nuclear polyhedrosis virus genome: physical maps for restriction endonucleases bamhi and hindiii. | the physical map for the genome of spodoptera frugiperda nuclear polyhedrosis virus was constructed for restriction endonucleases bamhi and hindiii. the ordering of the restriction fragments was accomplished by cross-blot hybridization of bamhi, hindiii, and ecori fragments. the alignment of the hindiii fragments within the bamhi map was achieved by double digestion with the two restriction endonucleases followed by cross-blot hybridization. the results showed that the viral genome consisted of ... | 1981 | 16789209 |
| construction of a genetic map of the baculovirus autographa californica nuclear polyhedrosis virus by marker rescue of temperature-sensitive mutants. | mutations of seven temperature-sensitive mutants of the baculovirus autographa californica nuclear polyhedrosis virus (npv) were mapped with respect to the physical restriction map of the a. californica npv dna by marker rescue. dnas from two distantly related npvs of the multiply embedded type and two npvs of the singly embedded type were unable to rescue two a. californica npv mutants. | 1981 | 16789213 |
| hybridization selection and in vitro translation of autographa californica nuclear polyhedrosis virus mrna. | we isolated polyadenylated rna from the cytoplasm of cells infected with autographa californica nuclear polyhedrosis virus late after infection (21 h postinfection). at that time intracellular protein synthesis was directed almost exclusively toward infected cell-specific proteins. the polyadenylic acid-containing rna sequences in the cytoplasm at 21 h postinfection were radiolabeled in vitro and hybridized to a. californica nuclear polyhedrosis virus dna restriction fragments. the polyadenylic ... | 1981 | 16789215 |
| orientation of the genome of autographa californica nuclear polyhedrosis virus: a proposal. | the nuclear polyhedrosis virus of the alfalfa looper autographa californica contains a double-stranded, circular dna genome. fourteen scientists agreed to accept an orientation of this circular genome with respect to a physical map of the restriction endonuclease cleavage sites. | 1982 | 16789218 |
| molecular cloning and physical mapping of restriction endonuclease fragments of autographa californica nuclear polyhedrosis virus dna. | a restriction fragment library containing autographa californica nuclear polyhedrosis virus (acnpv) dna was constructed by using the pbr322 plasmid as a vector. the library, which is representative of more than 95% of the viral genome, consists of 2 of the 7 bamhi fragments, 12 of the 24 hindiii fragments, and 23 of the 24 ecori fragments. the cloned fragments were characterized and used to generate physical maps of the genome by hybridizing nick-translated recombinant plasmid to southern blots ... | 1982 | 16789219 |
| capping of viral rna in cultured spodoptera frugiperda cells infected with autographa californica nuclear polyhedrosis virus. | viral rna from fall armyworm (spodoptera frugiperda) cells infected with autographa californica nuclear polyhedrosis virus contains cap structures. most of the cap labeled in vivo with [(3)h]methionine or (32)p(i) cochromatographed on deae-cellulose with the -5 charge marker; a minor component appeared at -4 net charge. the former is probably a cap 1 structure (m(7)gpppx(m) (p)yp), and the latter is probably a cap 0 (m(7)gpppxp). on the basis of relative labeling of the two caps with [(3)h]adeno ... | 1982 | 16789227 |
| mapping the mutation site of an autographa californica nuclear polyhedrosis virus polyhedron morphology mutant. | a polyhedron morphology mutant of autographa californica nuclear polyhedrosis virus, designated m5, was compared with wild-type virus by genotypic analysis with ecori, bamhi, hindiii, ssti, and smai restriction endonucleases. m5 dna revealed several alterations relative to the wild-type pattern: (i) ecori fragment i was 400 base pairs larger; (ii) bamhi fragment f was missing; (iii) hindiii fragment f was 400 base pairs larger; (iv) an extra restriction fragment was obtained with both hindiii an ... | 1982 | 16789229 |
| in vitro translation of autographa californica nuclear polyhedrosis virus early and late mrnas. | a preliminary translational map of the autographa californica genome was constructed. eighteen viral dna restriction fragments were either purified from agarose gels or obtained from pbr322 recombinant dna plasmids to locate specific gene products. the dnas were immobilized on nitrocellulose filters and used to select viral mrnas isolated from rna obtained from the cytoplasm of infected spodoptera frugiperda cells at 21 h postinfection. the fragment-specific mrnas were translated in vitro in the ... | 1982 | 16789231 |
| analysis of the spodoptera frugiperda nuclear polyhedrosis virus genome by restriction endonucleases and electron microscopy. | restriction endonuclease analysis was used to differentiate between four strains of spodoptera frugiperda nuclear polyhedrosis virus from different geographical areas. in addition, partial denaturation was performed, and a partial denaturation map was constructed for the ohio strain of this virus. | 1982 | 16789233 |
| physical analysis of autographa californica nuclear polyhedrosis virus transcripts for polyhedrin and 10,000-molecular-weight protein. | in autographa californica nuclear polyhedrosis virus-infected cells, polyhedrin, the major structural polypeptide of the viral occlusions, and a low-molecular-weight viral protein with a molecular weight of approximately 10,000 (10k) accumulated to high levels late in infection. two polyadenylated rnas 1,200 and 630 bases in size were the most abundant viral transcripts present in the cytoplasm at 48 h postinfection. evidence is presented that these rnas were the transcripts for polyhedrin and 1 ... | 1983 | 16789235 |
| location of homologous dna sequences interspersed at five regions in the baculovirus acmnpv genome. | an examination of autographa californica nuclear polyhedrosis virus dna revealed the presence of five interspersed regions, rich in ecori restriction sites, which shared homologous sequences. these homologous regions (hr), designated hr(1) to hr(5), occur at or near the following ecori fragment junctions: hr(1)ecori-b-ecori-i (0.0 map units); hr(2), ecori-a-ecori-j (19.8 map units); hr(3), ecori-c-ecori-g (52.9 map units); hr(4), ecori-q-ecori-l (69.8 map units); and hr(5), ecori-s-ecori-x (88.0 ... | 1983 | 16789237 |
| occluded and budded autographa californica nuclear polyhedrosis virus: immunological relatedness of structural proteins. | the immunological relatedness of the structural proteins of the budded and occluded phenotypes of autographa californica nuclear polyhedrosis virus was examined by reciprocal immunoblotting and by in situ peroxidase-antiperoxidase staining of virus-induced cell surface and intracellular antigens with antisera to both phenotypes. the molecular weights (mws) of major structural proteins of both phenotypes that reciprocally cross-reacted were 92,500, 78,000, 62,500, 54,000, and 42,000. a highly imm ... | 1983 | 16789239 |
| protein kinase activity associated with the extracellular and occluded forms of the baculovirus autographa californica nuclear polyhedrosis virus. | protein kinase activity is associated with both the extracellular and the occluded forms of autographa californica nuclear polyhedrosis virus, a baculovirus. serine and threonine are the predominant amino acids phosphorylated by the kinase activity associated with both viral forms; no phosphotyrosine was detected. the addition of calcium, camp, or cgmp has no apparent effect on the amount of phosphorylation or the substrates phosphorylated. | 1983 | 16789240 |
| molecular engineering of the autographa californica nuclear polyhedrosis virus genome: deletion mutations within the polyhedrin gene. | we describe a method to introduce site-specific mutations into the genome of autographa californica nuclear polyhedrosis virus. specifically, the a. californica nuclear polyhedrosis virus gene for polyhedrin, the major protein that forms viral occlusions in infected cells, was mutagenized by introducing deletions into the cloned dna fragment containing the gene. the mutagenized polyhedrin gene was transferred to the intact viral dna by mixing fragment and viral dnas, cotransfecting spodoptera fr ... | 1983 | 16789242 |
| viral transcription during autographa californica nuclear polyhedrosis virus infection: a novel rna polymerase induced in infected spodoptera frugiperda cells. | autographa californica nuclear polyhedrosis virus-specific rna synthesis in isolated nuclei of spodoptera frugiperda cells in culture was monitored at different times postinfection. up to 8 h postinfection viral rna synthesis remained sensitive to 5 mug of alpha-amanitin per ml. during the course of infection this sensitivity decreased, and at 24 h postinfection rna synthesis was completely resistant to alpha-amanitin. deae-sephadex profiles of rna polymerase isolated at 24 h postinfection showe ... | 1983 | 16789246 |
| mapping of early and late transcripts encoded by the autographa californica nuclear polyhedrosis virus genome: is viral rna spliced? | early and late transcripts were mapped on the autographa californica nuclear polyhedrosis virus genome by northern blotting and hybridization with the cloned viral ecori fragments. at least 11 early and about 90 late rnas were compared with over 32 polypeptides synthesized by in vitro translation of hybrid-selected rna. the latter method, of course, had its limitations also and did not guarantee that all viral rnas could be detected in this way. a comparison of cytoplasmic and total cellular rna ... | 1984 | 16789249 |
| time course analysis and mapping of autographa californica nuclear polyhedrosis virus transcripts. | to study the expression of the autographa californica nuclear polyhedrosis virus (acnpv) genome, intracellular virus-specific proteins and mrnas were pulsed-labeled, extracted, and analyzed at 6-h intervals during the replicative cycle. most rnas were detected between 12 and 24 h postinfection (p.i.), but many continued to be synthesized until late in infection. polyhedrin and p8 mrnas were the two most abundant late viral rna transcripts, and they were synthesized at high rates until late in th ... | 1984 | 16789250 |
| semipermissive replication of a nuclear polyhedrosis virus of autographa californica in a gypsy moth cell line. | several gypsy moth cell lines have been previously described as nonpermissive for the multiple-embedded nuclear polyhedrosis virus of autographa californica (acmnpv). in this report, we demonstrate the semipermissive infection of a gypsy moth cell line, iplb-ld-652y, with acmnpv. iplb-ld-652y cells infected with acmnpv produced classic cytopathic effects but failed to yield infectious progeny virus. results of experiments employing dna-dna dot hybridization suggested that acmnpv dna synthesis wa ... | 1986 | 16789253 |
| interspersed homologous dna of autographa californica nuclear polyhedrosis virus enhances delayed-early gene expression. | the five regions of homologous dna which are interspersed in the genome of the baculovirus autographa californica nuclear polyhedrosis virus increased the expression of a delayed-early gene of this virus. although this activity was first observed as a 10-fold trans effect, the homologous region 5 (hr5) enhanced the expression of linked genes 1,000-fold. the hr5 enhancer also exhibited the other characteristics associated with viral enhancer elements, including orientation independence and the ab ... | 1986 | 16789258 |
| complete sequence and enhancer function of the homologous dna regions of autographa californica nuclear polyhedrosis virus. | the nucleotide sequence of the five regions of homologous dna in the genome of autographa californica nuclear polyhedrosis virus dna was determined. the homology of repeated sequences within a region was 65 to 87%, and the consensus sequences for each region were 88% homologous to each other. sequences proximal to the ecori sites were most conserved, while the distal sequences were least conserved. the ecori sites formed the core of a 28-base-pair imperfect inverted repeat. all homologous region ... | 1986 | 16789259 |
| nucleotide sequence and temporal expression of a baculovirus regulatory gene. | the nucleotide sequence of a trans-activating regulatory gene (ie-1) of the baculovirus autographa californica nuclear polyhedrosis virus has been determined. this gene encodes a protein of 581 amino acids with a predicted molecular weight of 66,856. a dna fragment containing the entire coding sequence of ie-1 was inserted downstream of an rna promoter. subsequent cell-free transcription and translation directed the synthesis of a single peptide with an apparent molecular weight of 70,000. quant ... | 1987 | 16789264 |
| the autographa californica nuclear polyhedrosis virus acnpv induces functional maturation of human monocyte-derived dendritic cells. | the initiation of an adaptive immune response is critically dependent on the activation of dendritic cells (dcs). therefore, vaccination strategies targeting dcs have to ensure a proper presentation of the immunogen as well as an activation of dcs to accomplish their full maturation. viral vectors can achieve gene delivery and a subsequent presentation of the expressed immunogen, however, the immunization efficiency may be hampered by an inhibition of dc activation. here we report that the insec ... | 2006 | 16870312 |
| infection of spodoptera frugiperda cells with autographa californica nuclear polyhedrosis virus i. synthesis of intracellular proteins after virus infection. | the replication of autographa californica nuclear polyhedrosis virus (acnpv) in spodoptera frugiperda cells in culture has been studied with different methods. the first virus-induced polypeptides (with molecular weights of 46k, 30k, 29k) in infected cells appeared at 3 hr postinfection. viral dna synthesis started at about 5 hr postinfection. by electron microscopy, intranuclear nucleocapsids were detected at 10 hr postinfection and at about the same time, the titer of intracellular infectious ... | 1979 | 16945842 |
| infection of spodoptera frugiperda cells with autographa californica nuclear polyhedrosis virus ii. the viral dna and the kinetics of its replication. | the kinetics of replication of autographa californica nuclear polyhedrosis virus (acnpv) dna in spodoptera frugiperda cells in culture were studied. viral dna replication started at about 5 hr postinfection, the rate of viral dna replication reached a maximum at about 18 hr postinfection and thereafter decreased. parental viral dna could be detected within the first hour postinfection in the total intracellular dna by the southern technique. there was no evidence for the occurrence of acnpv dna ... | 1979 | 16945843 |
| infectious dna from autographa californica nuclear polyhedrosis virus. | cells from the lepidopteran spodoptera frugiperda can be successfully transfected in culture with the dna from autographa californica nuclear polyhedrosis virus (acnpv). the calcium chloride precipitation procedure has been used in conjunction with dimethyl sulfoxide treatment of the transfected cells. the highest specific infectivity observed was 6.1 x 10(4) pfu/mug of acnpv dna. as judged from a comparison of the restriction patterns of viral dna preparations, the virus produced in transfectio ... | 1980 | 16945844 |
| cloning and characterization of bombyx mori pp-bp a gene induced by viral infection. | the enf peptide family, so termed after the consensus sequence in their amino termini (glu-asn-phe-), is assumed to play multiple important roles in defense reactions, growth regulation, and homeostasis of lepidopteran insects. the paralytic peptide of bombyx mori (bmpp) is one such peptide that is involved in the paralytic and plasmatocyte-spreading activities in the hemocyte immune reaction. the growth-blocking peptide of pseudaletia separata (psgbp), which is also a member of the enf peptide ... | 2006 | 17112968 |
| p13 of leucania separata multiple nuclear polyhedrosis virus affected the polyhedra and budded virions yields of acmnpv. | p13 gene was first described by our laboratory in leucania separata multiple nuclear polyhedrovirus (ls-p13, orf114) back to 1995. however, the functions of ls-p13 and its reported homologues remained unknown. in order to probe the function of ls-p13, recombinant autographa californica nucleopolyhedroviruses (racmnpvs) were constructed to express ls-p13 in the sf9 cells at early, late or early/late phase. observations of microscope showed that the expression of ls-p13 could decrease the yield of ... | 2007 | 17141348 |
| enhanced effect of fluorescent whitening agent on peroral infection for recombinant baculovirus in the host bombyx mori l. | the low efficiency of the oral infectivity of recombinant polyhedrin-negative baculovirus is a major bottleneck in the application of the baculovirus expression system in the silkworm (bombyx mori l). in this study, the effects of a fluorescent whitening agent on improving the oral infection for the recombinant bombyx mori nuclear polyhedrosis virus in silkworm larva and their possible mechanism were investigated. the results showed that the peroral infection can be remarkably enhanced by adding ... | 2007 | 17160363 |
| optimization of baculovirus transduction on freestyle293 cells for the generation of influenza b/lee/40. | recombinant baculovirus expression vectors derived from the autographa californica nuclear polyhedrosis virus can serve as efficient gene-transfer vehicles for transient expression of recombinant proteins in a wide range of mammalian cell types and are able to produce multisubunit particles such as viruses or virus like particles. in this study, we constructed eight recombinant baculoviruses each containing one of the influenza b/lee/40 virus genes in a bidirectional expression cassette for simu ... | 2006 | 17172661 |
| [improved efficient gene transfer into insect and mammalian cells by baculovirus vectors]. | the fragments of genomics dna of the nuclear polyhedrosis virus (npv) containing genes of late viral proteins p10, p35, p39, were cloned, the promoter regions of this genes were used to design baculovirus transfer vectors. a double-promoter and triple-promoter baculovirus transfer vectors were obtained. recombinant baculovirus vectors containing mammalian expression cassette with cytomegalovirus (cmv) promoter, the gene for green or red fluorescent protein, sv40pa and polylinker mcs were constru ... | 2006 | 17209436 |
| function and oligomerization study of the leucine zipper-like domain in p13 from leucania separata multiple nuclear polyhedrosis virus. | the p13 gene is uniquely present in group ii nucleopolyhedroviruses (npvs) and some granuloviruses, but not in group i npvs. p13 gene was first described by our laboratory in leucania separatamultiple nuclear polyhedrosis virus (ls-p13) in 1995. however, the functions of ls-p13 and of its homologues are unknown. when ls-p13 was inserted into autographa californica nucleopolyhedrovirus, a group i npv, polyhedra yield was inhibited. however, this inhibition was prevented when the leucine zipper-li ... | 2007 | 17394774 |
| a highly efficient method for the generation of a recombinant bombyx mori nuclear-polyhedrosis-virus bacmid and large-scale expression of foreign proteins in silkworm (b. mori) larvae. | in the post-genomic era, one of the challenges and a source of competition is the development of high-throughput, large-scale and low-cost eukaryotic cdna cloning and expression systems. the baculovirus expression system is the most popular one and plays an important role in the high-level expression of eukaryotic proteins. in the present study, a convenient, rapid and highly efficient method for the construction of recombinant bmnpv (bombyx mori nuclear polyhedrosis virus)-bacmid vector (bmbacm ... | 2007 | 17428194 |
| influence of cytochrome c on apoptosis induced by anagrapha (syngrapha) falcifera multiple nuclear polyhedrosis virus (afmnpv) in insect spodoptera litura cells. | we investigated the influence of cytochrome c on apoptosis induced by anagrapha (syngrapha) falcifera multiple nuclear polyhedrosis virus (afmnpv). microscopic observation revealed that infection of sl-1 cells with afmnpv resulted in apoptosis, displaying apoptotic bodies in fluorescent-stained nuclei of afmnpv-infected sl-1cells. western blot analysis demonstrated that afmnpv-induced apoptosis in insect sl-1 cells was significantly inhibited by cyclosporin a which blocked a translocation of cyt ... | 2007 | 17478109 |
| purification and characterization of a recombinant rat prohaptoglobin expressed in baculovirus-infected sf9 insect cells. | to generate hemoglobin-free full-length haptoglobin the cdna encoding rat haptoglobin alphabeta subunits was cloned into shuttle vector pvt-bac-his and used to produce a recombinant baculovirus autographa californica nuclear polyhedrosis virus (acnpv) as an expression vector, named hpacnpv. recombinant virus was used to infect spodoptera frugiperda (sf9) insect cells. the 50 kda protein expressed was mostly secreted into the culture medium at relatively high titer (15 microg/ml) and was found to ... | 2007 | 17681809 |
| the chitinase a from the baculovirus acmnpv enhances resistance to both fungi and herbivorous pests in tobacco. | biotechnology has allowed the development of novel strategies to obtain plants that are more resistant to pests, fungal pathogens and other agents of biotic stress. the obvious advantages of having genotypes with multiple beneficial traits have recently fostered the development of gene pyramiding strategies, but less attention has been given to the study of genes that can increase resistance to different types of harmful organisms. here we report that a recombinant chitinase a protein of the aut ... | 2008 | 17851776 |
| cloning and expression analysis of heat shock cognate 70 gene promoter in tiger shrimp (penaeus monodon). | heat shock cognate 70 (hsc70) functions as a molecular chaperon and plays an important role in protein folding. hsc70 cdna of tiger shrimp (penaeus monodon) was cloned and characterized in our previous study. after shrimps were treated with the 1-hr heat shock, the hsc70 mrna level in hemocytes increased (approximately 8 fold) using real-time quantitative pcr. an hsc70 clone was obtained from genomic library screening. the gene contains 2 exons separated by a 1557-bp intron. the 5'-flanking regi ... | 2007 | 17931801 |
| induction of antitumor acquired immunity by baculovirus autographa californica multiple nuclear polyhedrosis virus infection in mice. | the baculovirus autographa californica multiple nuclear polyhedrosis virus (acmnpv) has been studied as a gene therapy vector. here, we demonstrated that acmnpv induces antitumor acquired immunity. these results suggest that acmnpv has the potential to be an efficient virus or tumor therapy agent which induces innate and acquired immunity. | 2008 | 18057182 |
| induction of natural killer cell-dependent antitumor immunity by the autographa californica multiple nuclear polyhedrosis virus. | wild-type autographa californica multiple nuclear polyhedrosis virus (acmnpv) infects a variety of mammalian cell types in vitro, but does not replicate in these cells. we investigated the effects of acmnpv in the induction of the immune response and tumor metastasis in mice. after intravenous injection, acmnpv was taken up by the liver and spleen, and preferentially infected dendritic cells (dcs) and b cells in the spleen; costimulatory molecules cd40, cd80, and cd86 were upregulated in the dcs ... | 2008 | 18059370 |
| the effects of bmnpv on biochemical changes in primary cultures of bombyx mori embryonic tissue. | the effect of bombyx mori nuclear polyhedrosis virus (bmnpv) on biochemical changes of tc-100 medium containing 10% fetal bovine serum (fbs) in embryonic primary cultures of silkworm was investigated. the primary cultures that reached 60% confluence were infected by 0.5, 1, and 2-ml viral inoculums (diluted with tc-100 medium representing multiplicity of infection (moi) of 0.25, 0.5, and 1). glucose, uric acid, urea, total protein, cholesterol, and alkaline phosphatase were measured in the mediu ... | 2008 | 18288542 |
| acmnpv ac143 (odv-e18) is essential for mediating budded virus production and is the 30th baculovirus core gene. | autographa californica multiple nucleopolyhedrovirus (acmnpv) ac143 (odv-e18) is a late gene that encodes for a predicted 9.6 kda structural protein that locates to the occlusion derived viral envelope and viral induced intranuclear microvesicles [braunagel, s.c., he, h., ramamurthy, p., and summers, m.d. (1996). transcription, translation, and cellular localization of three autographa californica nuclear polyhedrosis virus structural proteins: odv-e18, odv-e35, and odv-ec27. virology 222, 100-1 ... | 2008 | 18328526 |
| baculovirus-mediated interferon alleviates dimethylnitrosamine-induced liver cirrhosis symptoms in a murine model. | the wild-type baculovirus autographa californica multiple nuclear polyhedrosis virus (acmnpv) infects a range of mammalian cell types in vitro but does not replicate in these cells. the current study investigated the in vivo effect of acmnpv in the mouse model of liver cirrhosis induced by the mutagen dimethylnitrosamine. intraperitoneal injection of acmnpv induced an immune response. the baculovirus was taken up by the liver and spleen where it suppressed liver injury and fibrosis through the i ... | 2008 | 18369328 |
| nanosilica-from medicine to pest control. | nanotechnology is a broad interdisciplinary area of research, development, and industrial activity that has been growing rapidly worldwide for the past decade. more ambitious uses of nanoparticles are bioremediation of contaminated environments, controlled release of fragrances, biocides, and antifungals on textiles. silica nanocomposites have received much attention because of its thermal degradation behavior and applications in chromatography, medicine, optics, etc. nanobiotech takes agricultu ... | 2008 | 18438740 |
| quantitative analysis of cytoplasmic actin gene promoter and nuclear polyhedrosis virus immediate-early promoter activities in various tissues of silkworm bombyx mori using recombinant autographa californica nuclear polyhedrosis virus as vector. | cassettes harboring luciferase reporter driven by bombyx mori cytoplasmic actin gene promoter (a3) (671 bp) and b. mori nuclear polyhedrosis virus immediate-early promoter (ie-1) (580 bp) were transferred to the bacmid acdeltaegt to generate the recombinant autographa californica nuclear polyhedrosis viruses, acnpva3luc and acnpvieluc, respectively. recombinant baculoviruses were injected into the hemocoele of newly ecdysed 5th instar larvae. the activities of the a3 and ie-1 promoters in variou ... | 2008 | 18535752 |
| alginate concentration: a key factor in growth of temperature-sensitive baculovirus-infected insect cells in microcapsules. | the desire to increase cell density and product concentration has been the primary driving force for the development of better animal cell culture processes. in the technique used in our laboratory-microencapsulation-insect cells (spodoptera frugiperda), infected with a temperature-sensitive mutant of the autographa californica nuclear polyhedrosis virus (acnpv), were cultured in multiple membrane alginate-polylysine (pll) microcapsules which had a controlled membrane molecular-weight cutoff and ... | 1989 | 18588202 |
| production of a discrete, heterogeneous population of beta-galactosidase polypeptides using baculovirus expression vectors. | gel electrophoresis analysis of immunoprecipitated beta-galactosidase and polyhedrin-beta-galactosidase expressed in spodoptera frugiperda cells infected with recombinant autograph californica nuclear polyhedrosis virus revealed the existence of a population of discrete beta-galactosidase polypeptides. several of the polypeptides observed in the fusion protein expression experiments exhibit a consistent pattern of slightly greater molecular weight when compared to the nonfusion beta-galactosidas ... | 1992 | 18601031 |
| investigation of reduced serum and serum-free media for the cultivation of insect cells (bm5) and the production of baculovirus (bmnpv). | an experimental study has been carried out to investigate the effectivenes of several reduced serum and serum-free media for the cultivation of an ovarian cell line, bm5, of the lepidopteran insect bombyx mori. bm5 cell were successfully adapted to grow in a medium containing 5% serum and a serum-free medium (ex-cell 400). on the other hand, this cell line could not be adapted to grow in several other media suggested in the literature, including ipl-41 + 2% fetal bovine serum (fbs), sf-900, and ... | 1992 | 18601067 |
| a structured dynamic model for the baculovirus infection process in insect-cell reactor configurations. | a mathematical description of the infection of insect cells with baculovirus in a continuously operated reactor configuration is presented. the reactor configuration consists of one bioreactor in which insect cells (spodoptera frugiperda) are grown followed by one or two bioreactors in which cells are infected by a baculovirus (autographa californica nuclear polyhedrosis virus). it was demonstrated that the so-called passage effect is responsible for the observed difference in run time between a ... | 1992 | 18601149 |
| transfer vectors for maximal expression of passenger genes in the bombyx mori nuclear polyhedrosis virus expression system. | a series of bombyx mori nuclear polyhedrosis virus (bm-npv) transfer vectors has been developed containing various lengths of the polyhedrin promoter, including sequences 3' of the initiation codon. the atg initiation codon was mutated in some of these vectors to allow for the production of authentic nonfusion proteins. the ability of the various polyhedrin promoter constructs to direct expression of foreign gene sequences was assessed using two test genes, chloramphenicol acetyl transferase (ca ... | 1993 | 18612957 |
| production of recombinant proteins in high-density insect cell cultures. | the effect of the growth phase of spodoptera frugiperda (sf9) cells on the production of recombinant proteins (beta-galactosidase and glucocerebrosidase) was investigated. cells infected with the recombinant autographa californica nuclear polyhedrosis virus at the late exponential and stationary phases yielded low quantities of expressed protein. highest enzyme yields were obtained using sf9 cells from the early exponential phase (0.9 mg beta-galactosidase/10(6) cells and 1.7 microg glucocerebro ... | 1993 | 18612984 |
| a two-stage bioreactor system for the production of recombinant proteins using a genetically engineered baculovirus/insect cell system. | a two-stage bioreactor scheme was developed for the large-scale production of recombinant proteins using a genetically engineered baculovirus/insect cell system. the first bioreactor was employed for cell growth and the second for cell infection. silkworm bm5 cells were infected with a recombinant baculovirus, bmnpv/p5.cat, containing a bacterial chloramphenicol acetyltransferase (cat) gene under the control of the polyhedrin gene promoter of bombyx mori nuclear polyhedrosis virus (bmnpv). this ... | 1993 | 18613020 |
| analysis of baculovirus genomes with restriction endonucleases. | the viral dnas from nine wild-type insect baculoviruses have been isolated and the ecor-1 restriction endonuclease fragment patterns compared. genomic heterogeneity could be detected in the dna restriction patterns of four of these wild-type baculoviruses. three infectious virus forms (two that are occluded in the nucleus and an extracellular virus that has budded from the plasma membrane of infected cells) of a nuclear polyhedrosis virus with multiple nucleocapsids per envelope (mnpv) from the ... | 1978 | 18627885 |
| characteristics of the dna from lymantria dispar nuclear polyhedrosis virus. | the dna isolated from occluded lymantria dispar nuclear polyhedrosis virus consisted of double-stranded linear, open circular, and covalently closed molecules. the dna had a guanosine plus cytosine content (g + c) of 59-62% as estimated from both thermal denaturation and cscl density gradient centrifugation. the l. dispar host dna had a g + c content of 38-39%. comparisons of molecular weight estimations for viral dna by sedimentation on neutral sucrose gradients, electron microscopy, and reasso ... | 1979 | 18627902 |
| on the origin of the polyhedral protein of autographa californica nuclear polyhedrosis virus. isolation, characterization, and translation of viral messenger rna. | virus-specific messenger rna was isolated 24 hr postinfection from spodoptera frugiperda cells infected with autographa californica nuclear polyhedrosis virus (acnpv) by means of hybridization with viral dna that was coupled to cellulose. viral mrna was analyzed on isokinetic sucrose gradients and on polyacrylamide gels containing 98% formamide. viral mrna was also translated in an in vitro protein-synthesizing system derived from wheat germ. one product comigrated with purified acnpv polyhedral ... | 1980 | 18631636 |
| autographa californica nuclear polyhedrosis virus-induced proteins in tissue culture. | the multiplication of autographa californica nuclear polyhedrosis virus was studied in trichoplusia ni and spodoptera frugiperda insect cell lines. using pulse labeling, 18 viral-induced proteins were identified by polyacrylamide gel electrophoresis. based on infectivity curves and protein synthesis studies, replication could be divided into four phases. during the early phase of infection, four viral-induced proteins with molecular weights of 45, 35, 34, and 31 thousand daltons could be detecte ... | 1980 | 18631644 |
| structural analysis of the matrix protein from the nuclear polyhedrosis virus of heliothis zea. | polyhedrin from the nuclear polyhedrosis virus of heliothis zea was analyzed. alkali-solubilized polyhedrin consists of a 12 s aggregate of 27,000 mw subunits. results from chemical crosslinking experiments suggest that 12 subunits are present in the 12 s aggregate. in isoelectric focusing gels, the aggregate migrates as a single entity with an isoelectric point of 5.9. under denaturing conditions, four charge isomers of the subunits are revealed. the presence of alkaline protease activity in th ... | 1980 | 18631649 |
| protease degradation of autographa californica nuclear polyhedrosis virus proteins. | the autographa californica nuclear polyhedrosis virus (ac-npv) occlusion bodies produced in vivo contained a protease which degraded occlusion body matrix protein and polypeptides of enveloped nucleocapsids during alkaline dissolution. the protease activity was required for complete dissociation of occlusion body matrix protein from the viral membrane during alkali liberation of the virus from occlusion bodies. in addition protease activity was required for the subsequent removal of the viral me ... | 1980 | 18631654 |
| protein synthesis in cells infected by autographa californica nuclear polyhedrosis virus (ac-npv): the effect of cytosine arabinoside. | twenty-two virion polypeptides (vp) were detected reproducibly when the occluded form (ov) of [35s]methionine-labeled ac-npv, purified from polyhedral inclusion bodies (pib), was analyzed by polyacrylamide gel electrophoresis and autoradiography. ten of the vps and the polyhedral protein (pp) were phosphorylated and 6 vps were glycoproteins. purified, nonoccluded virus (nov) revealed 25 polypeptides. during a single cycle of virus replication, the synthesis of 33 infected cell polypeptides (1cp) ... | 1980 | 18631655 |
| isolation and replication of an occlusion body-deficient mutant of the autographa californica nuclear polyhedrosis virus. | a spontaneous mutant of the autographa californica nuclear polyhedrosis virus which was deficient in viral occlusion body formation was studied in trichoplusia ni tissue culture cells. virus multiplication could be divided into four phases based on growth curves and the sequential appearance of 25 viral-induced proteins identified by pulse labeling and polyacrylamide gel electrophoresis. during the latent phase, five viral-induced proteins with molecular weights of 68, 47, 35, and 30 thousand (k ... | 1980 | 18631676 |
| physicochemical properties and location of capsule components, in particular the synergistic factor, in the occlusion body of a granulosis virus of the armyworm, pseudaletia unipuncta. | a synergistic strain of granulosis virus (gvh) enhances the infection of larvae of the armyworm, pseudaletia unipuncta, with a nuclear polyhedrosis virus (npv). the matrix of the capsule (occlusion body) of gvh contains two major components, the structural protein and the synergistic factor. the structure of the capsule matrix of gvh was analyzed with a primary amine-coupling reagent, 2,4,6,-trinitrobenzene 1-sulfonic acid (tnbs). when undissolved capsules are incubated with tnbs, only the struc ... | 1980 | 18631836 |
| in vivo pathway of autographa californica baculovirus invasion and infection. | the pathway of autographa californica nuclear polyhedrosis virus (acnpv) infection in cabbage looper, trichoplusia ni, larval midgut cells was studied by ultrastructural and virus titration methods. enveloped virions interacted with microvilli of columnar cells resulting in apparent fusion of the viral envelope and microvillus membrane. after entry into the cell cytoplasm, the intact nucleocapsids appeared to enter the nucleus through nuclear pores, and uncoating of the viral genome took place i ... | 1981 | 18635031 |
| autographa californica nuclear polyhedrosis virus phosphoproteins and synthesis of intracellular proteins after virus infection. | polypeptides and phosphoproteins isolated from nuclear and cytoplasmic fractions of tn-368-10 cells infected with autographa californica nuclear polyhedrosis virus (acmnpv) were analyzed by polyacrylamide gel electrophoresis. likewise, acmnpv extracellular virus and alkali-released virus were compared. acmnpv extracellular virus possessed at least nine phosphoproteins while the alkali-released virus had about 14 phosphoproteins. the major structural polypeptide of acmnpv, polyhedrin, was phospho ... | 1981 | 18635034 |
| investigation of genetic heterogeneity in wild isolates of spodoptera frugiperda nuclear polyhedrosis virus by restriction endonuclease analysis of plaque-purified variants. | spodoptera frugiperda nuclear polyhedrosis virus (sfmnpv was obtained from four different sources and each isolate was cultured in iplb-sf21 cells. viral dna from each isolate was analyzed with ecor1, xho1, and bamh1 restriction endonucleases. all four isolates could be distinguished on the basis of minor differences in ecor1 patterns. submolar dna restriction fragments were seen in all isolates indicating that each isolate consisted of a mixture of different genotypes. to investigate this, two ... | 1981 | 18635067 |
| cell culture studies with the imc-hz-1 nonoccluded virus. | studies were conducted on an adventitious agent (hz-1v) isolated from the imc-hz-1 cell line. it appeared identical to the virus first obtained by granados et al (r. r. granados, t. nguyen, and b. cato, 1978, intervirology, 10, 309-317) from a persistent infection of this cell line. restriction endonuclease digestion of hz-1v dna indicated the agent was different from the s nuclear polyhedrosis virus (hzsnpv) of the host species, heliothis zea, from which the imc-hz-1 cell line was derived by hi ... | 1981 | 18635105 |
| the isolation and characterization of the mp and fp plaque variants of galleria mellonella nuclear polyhedrosis virus. | two plaque morphologies were detected in assays of galleria mellonella nuclear polyhedrosis virus preparations which had been serially passaged in the tn-368 cell line. the fp (few polyhedra) plaques were larger (0.7-mm average diameter) and the infected cells contained less than 10 polyhedra each. the mp (many polyhedra) plaques were smaller (0.5-mm average diameter), and the infected cells contained greater than 10 each. only the fp variant could be purified by plaque picking and remained pure ... | 1982 | 18635122 |
| dna restriction-pattern differences from geographic isolates of spodoptera littoralis nuclear polyhedrosis virus. | two nuclear polyhedrosis viruses were isolated from spodoptera littoralis larvae collected from several areas in israel. the two viruses were characterized and compared by restriction endonuclease cleavage of their dna and southern blot hybridization. the two viruses have no detectable sequence homology. a variant, homologous to the more common virus isolate, was also found. | 1982 | 18635146 |
| alteration of autographa californica nuclear polyhedrosis virus dna upon serial passage in cell culture. | plaque-purified autographa californica nuclear polyhedrosis virus, acmnpv e2 isolate, was propagated for 30 generations by serial passage in tn-368 cells. after passage, the dnas from 7 of 20 replaque-purified isolates were found to have additional ecori restriction enzyme fragments. these 7 isolates were of three types. the additional ecori fragments of each isolate hybridized to existing viral dna. restriction enzyme mapping with saci and smai show that isolates of each type contain an inserti ... | 1982 | 18635147 |
| inhibition of autographa californica nuclear polyhedrosis virus replication in high-density trichoplusia ni cell cultures. | replication of the autographa californica nuclear polyhedrosis virus was studied in trichoplusia ni (tn-368) tissue culture cells under cell density conditions which approximated a confluent monolayer (5.7 x 10(5) cells/cm(2) growth surface). these high-density cultures were not refractory to infection but the synthesis of both the occluded and nonoccluded forms of the virus were inhibited greater than 98% when compared to inoculation of low cell density cultures (1.4 x 10(5) cells/cm(2)). the i ... | 1982 | 18635148 |
| expression of cell surface gpi-anchored human p97 in baculovirus-infected insect cells. | the baculovirus/insect cell system (autographa californica multiple nuclear polyhedrosis virus/spodoptera frugiperda sf9 cells) was used to express the gpi-anchored human melanoma tumor antigen, melanotransferrin or p97. this system served to study the expression and productivity of recombinant gpi-anchored p97 by insect cells. the sf9 cells expressed a cell surface gpi-anchored form of p97 as well as a soluble form of p97 that did not appear to be derived from the gpi-anchored form of p97. both ... | 1997 | 18636443 |
| substrate limitation in the baculovirus expression vector system. | the inability to infect insect cell cultures at the highest achievable cell densities has imposed major limitations to both the fundamental understanding of the baculovirus expression vector system (bevs) as well as full exploitation of its potential productive capacity for recombinant (beta-galacnpv) products. the current literature does not characterize and identify the exact nature of the observed limitations, which therefore has become the major objective and contribution of the following st ... | 1997 | 18636607 |
| expression of green fluorescent protein in insect larvae and its application for heterologous protein production. | many eukaryotic proteins have been successfully expressed in insect cells infected with a recombinant baculovirus derived from the autographa californica nuclear polyhedrosis virus (acnpv). there are, however, disadvantages with this cell-based system when carried out in suspension cultures at high bioreactor volume (e.g., limited oxygen transfer, susceptibility to contamination, high cost). these problems can be avoided by using whole larvae as the "reactors." there are, however, other problems ... | 1997 | 18636639 |
| properties of the nuclear polyhedrosis virus of the great wax moth: oligomeric circular dna and the characteristics of the genome. | | 1982 | 18638732 |
| identification, cloning, and r-loop mapping of the polyhedrin gene from the multicapsid nuclear polyhedrosis virus of orgyia pseudotsugata. | polyadenylated rna was isolated from orgyia pseudotsugata larvae 8-10 days postinfection with the multicapsid nuclear polyhedrosis virus. this rna was centrifuged through a sucrose gradient and fractions enriched for polyhedrin mrna were identified by in vitro translation. complementary dna made to this rna hybridized predominantly to a 5-kb fragment of xhoi-digested viral dna. this fragment was cloned into the plasmid pacyc177 and mapped with restriction endonucleases. a sali subclone with a 2. ... | 1982 | 18638748 |
| characterization of monoclonal antibodies to the autographa californica nuclear polyhedrosis virus. | mouse hybridomas which produce monoclonal antibodies to the autographa californica nuclear polyhedrosis virus (acnpv) have been prepared and the protein to which each is directed has been determined. the antibody produced by clones 3f3 and 5e9 specifically bound polyhedron protein of mw 32,000 when used in immunoadsorption chromatography when used in elisa they could not distinguish between insect- and cell culture-derived polyhedron protein and also reacted with a wide range of baculoviruses. t ... | 1982 | 18638810 |
| dna homology among subgroup a, b, and c baculoviruses. | the dna homology among 18 baculoviruses from subgroups a, b, and c was compared using restriction endonuclease analysis, dna hybridization, and southern blot hybridization. restriction enzyme patterns for each of the viral dnas compared was unique. viral dna homology was evaluated by hybridizing 32p-labeled viral dnas to unlabeled dnas immobilized on nitrocellulose filters. hybridization conditions were varied by using a range of formamide concentrations during annealing. the apparent dna homolo ... | 1982 | 18638848 |
| characterization of two abundant mrnas of autographa californica nuclear polyhedrosis virus present late in infection. | cytoplasmic poly(a)(+) rna isolated from spodoptera frugiperda cells late after infection with autographa californica nuclear polyhedrosis virus (30-40 hr pi) was fractionated according to size on denaturing methyl mercury gels. two major rna species (1.4 kb and 0.75 kb) and several minor rna species were detected by ethidium bromide staining. the predominant rna species of about 1.4 kb was considered to be polyhedrin mrna because (1) in vitro translation of the rna, which was eluted from methyl ... | 1983 | 18638868 |
| a temperature-sensitive mutant of the baculovirus autographa californica nuclear polyhedrosis virus defective in an early function required for further gene expression. | fifteen temperature-sensitive (ts) mutants of the baculovirus autographa californica nuclear polyhedrosis virus (acnpv) have been analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of proteins synthesized in infected cells. one of the mutants, tsb821, was found to be defective in a very early function. seven virus-induced proteins were synthesized by 2 hr postinfection. in marked contrast to wild-type virus and the other 14 ts mutants, the synthesis of further virus ... | 1983 | 18638939 |
| mapping early transcription products of autographa californica nuclear polyhedrosis virus. | the regions of the acmnpv genome represented as cytoplasmic transcripts early after infection of spodoptera frugiperda cells prior to and encompassing the initiation of dna replication were mapped. in vivo(32)p pulse-labeled cytoplasmic rna from infected cells at various times postinfection was used to probe southern blots of cloned ecori acmnpv dna fragments. at the earliest time point studied (0.5 - 2.5 hr p.i.) transcripts represented a large proportion of the genome although specific regions ... | 1983 | 18638940 |
| a semipermissive nuclear polyhedrosis virus infection: characterization of infection kinetics and morphogenesis. | an in vitro host-range model system, designated system ii, was developed for the multicapsid nuclear polyhedrosis virus spodoptera frugiperda (sf-mnpv). this system consisted of sf-mnpv-infected s. frugiperda cells (permissive system) and sf-mnpv-infected trichoplusia ni cells (semipermissive system). infection kinetic studies revealed that while progeny virus was produced in the semipermissive system, the infectious virus yield was at least 700-fold lower than that in positive controls. electro ... | 1983 | 18639138 |
| a study of the glycoproteins of autographa californica nuclear polyhedrosis virus (acnpv). | pulse labeling with tritiated mannose was used to follow the time course of autographa californica nuclear polyhedrosis virus (acnpv) glycoprotein synthesis in spodoptera frugiperda iplb-21 cells. nine viral-induced intracellular glycoproteins were first detected from as early as 2 hr postinoculation (67k, early phase) to as late as 14 hr (36k and 19k glycoproteins, intermediate phase). glycosylation of these proteins was observed to continue to the end of the experiment (28 hr postinoculation). ... | 1983 | 18639173 |
| nucleotide sequence of the polyhedrin gene of autographa californica nuclear polyhedrosis virus. | the nucleotide sequence of a 1143-bp region of autographa californica mnpv (acmnpv) dna containing the entire coding region of polyhedrin was determined. the coding region consisted of 732 bp without intervening sequences. the 5' leader sequence was approximately 58 nucleotides in length. a putative "tata box" and "caat box" were located 20 and 52 bp upstream from the transcriptional start site, respectively. two tandem repeats of 8 bp were found 70 and 84 bp upstream from the transcriptional st ... | 1983 | 18639177 |
| regions of repeated dna in the genome of choristoneura fumifefana nuclear polyhedrosis virus. | the genome of the nuclear polyhedrosis virus of the spruce budworm, choristoneura fumiferana (cfmnpv), was shown, by southern blot hybridization, to contain regions of repeated dna sequences. these homologous regions have been located at four sites on the physical map of cfmnpv. in addition, the dna at one of these sites is expanded, possibly via tandem duplication, in a fraction of the total viral dna population. | 1984 | 18639829 |
| budded autographa californica npv 64k protein: further biochemical analysis and effects of postimmunoprecipitation sample preparation conditions. | previously it was shown that acv1, a neutralizing monoclonal antibody of the autographa californica nuclear polyhedrosis virus-budded phenotype reacted with a surface antigen present on infected cells during virus budding, and in the viral envelope (l. e. volkman, p. a. goldsmith, r. t. hess, and p. faulkner (1984), virology 133, 354-362). radioimmune precipitation of solubilized, [35s]methionine-labeled budded virus with acv1 and analysis on sds-page revealed four bands consistently: one major ... | 1984 | 18639832 |
| nucleotide sequence of the p10 polypeptide gene of autographa californica nuclear polyhedrosis virus. | a portion of the autographa californica nuclear polyhedrosis virus genome lying within the ecori-p region of the physical map has been sequenced. previous studies employing northern blotting and in vitro translation had shown that this region encoded the gene for a "late" 8-10k polypeptide [d. z. rohel, m. a. cochran, and p. faulkner, virology 124, 357-365, (1983); g. e. smith, j. m. vlak, and m. d. summers, j. virol.45, 215-225, (1983)]. the 1313-base pair region contained an open reading frame ... | 1984 | 18639833 |
| mechanism of neutralization of budded autographa californica nuclear polyhedrosis virus by a monoclonal antibody: inhibition of entry by adsorptive endocytosis. | autographa californica nuclear polyhedrosis virus (acnpv) is characterized by two different phenotypes, each with a specific role in the life cycle of the virus in nature. they differ widely in infectivity both in vivo and in vitro, and are neutralized by different populations of antibodies. a monoclonal antibody designated acv1 neutralizes one phenotype, bv, by reacting with a major envelope antigen not present on the other phenotype. bv can functionally enter cells by two different pathways an ... | 1985 | 18639849 |
| monoclonal antibodies identify conserved epitopes on the polyhedrin of heliothis zea nuclear polyhedrosis virus. | monoclonal antibodies were formed against polyhedrin from the heliothis zea s nuclear polyhedrosis virus. the cross-reactivity of these antibodies with polyhedrins and granulins of other occluded baculoviruses indicates that group and subgroup epitopes exist. evidence is presented that multiple copies of one epitope or a series of closely related epitopes exist on the polyhedrin molecule. the monoclonal antibodies, with the exception of one igm-producing hybridoma clone, were all of the igg1 iso ... | 1985 | 18639853 |
| alternate pathway of entry of budded autographa californica nuclear polyhedrosis virus: fusion at the plasma membrane. | recently we reported that the budded phenotype of autographa californica nuclear polyhedrosis virus (aenpv bv) functionally entered iplb-sf-21 cells by adsorptive endocytosis and that neutralizing monoclonal antibody acv1 acted by preventing acnpv bv from using this entry pathway (l. e. volkman and p. a. goldsmith (1985), virology 143, 185-195). not all infectivity could be neutralized by acv1, however, and in the study reported herein we investigated the nature of the particles responsible for ... | 1986 | 18640581 |
| replication of the trichoplusia ni granulosis and nuclear polyhedrosis viruses in cell cultures. | newly established trichoplusia ni (cabbage looper) embryonic cell lines were infected with t. ni granulosis virus (tngv) and t. ni nuclear polyhedrosis virus (tnsnpv). infection of cultured cells with tngv was ascertained by peroxidase-antiperoxidase staining, dna slot-blot hybridization, and transmission electron microscopy. initially, 15 cell lines supported tngv replication, the percentage of infected cells ranging from 1 to 50%.however, susceptibility of the 15 cell lines to tngv infection e ... | 1986 | 18640644 |
| effect of tunicamycin on the structural proteins and infectivity of budded autographa californica nuclear polyhedrosis virus. | the budded phenotype of autograha californica nuclear polyhedrosis virus (acnpv bv) has a 64k glycoprotein in its envelope which is important in the ability of the virus to functionally enter host cells. tunicamycin (tm), an inhibitor of n-linked glycosylation, was used to determine whether the sugar moiety was essential for the normal function of the 64k protein. at 10 mug/ml tm, glycosylation was completely inhibited. infectivity of acnpv bv was reduced to 2% of control while extracellular par ... | 1986 | 18640650 |
| bcl-2 expression in spodoptera frugiperda sf-9 and trichoplusia ni bti-tn-5b1-4 insect cells: effect on recombinant protein expression and cell viability. | the effect of bcl-2 expression on cell viability and recombinant protein synthesis was investigated in the spodoptera frugiperda sf-9 and trichoplusia ni bti-tn-5b1-4 (high fivetrade mark) insect cell lines. it was found that coinfection with a baculovirus expressing bcl-2 [autographa californica nuclear polyhedrosis virus (acnpv)-bcl2] extended the life span of high fivetrade mark cells but not sf-9 cells when compared to infection with recombinant baculoviruses expressing either human tissue p ... | 1997 | 18642241 |
| evidence for microfilament involvement in budded autographa californica nuclear polyhedrosis virus production. | autographa californica nuclear polyhedrosis virus (acnpv) is a large, double-stranded dna virus that infects lepidopteran insects. in the course of infection two different forms of the virus are produced; one that serves to spread the infection from insect to insect and another that spreads the infection within the insect from cell to cell. the latter form matures by budding from the plasma membrane of infected cells. we have found that cytochalasins b and d prevent the production of infectious ... | 1987 | 18644552 |
| a novel way to purify recombinant baculoviruses by using bacmid. | in the present study, we studied the feasibility of deleting essential genes in insect cells by using bacmid and purifying recombinant bacmid in escherichia coli dh10b cells. to disrupt the orf4 (open reading frame 4) gene of bmnpv [bm (bombyx mori) nuclear polyhedrosis virus], a transfer vector was constructed and co-transfected with bmnpv bacmid into bm cells. three passages of viruses were carried out in bm cells, followed by one round of purification. subsequently, bacmid dna was extracted a ... | 2009 | 18684105 |
| baculovirus up-regulates antiviral systems and induces protection against infectious bronchitis virus challenge in neonatal chicken. | in this study, the antheraea pernyi nuclear polyhedrosis virus (apnpv), a member of the baculovirus family, is evaluated for its stimulation of chicken peripheral blood mononuclear cells (pbmc) and macrophage cell line hd 11 in vitro, and protection against infectious bronchitis virus (ibv) in neonatal chickens in vivo. this study showed that apnpv significantly enhanced inflammatory cytokine mrna expression in chicken pbmc and hd 11 cells through the clathrin-dependent endocytic and endosomal m ... | 2008 | 18707025 |
| baculovirus-mediated gene transfer into mammalian cells. | a kind of cytotoxinic gene barnase was cloned downstream of immediate early promoter from cytomegalovirus (cmv). recombinant virus vaccmvbar was constructed using bac-to-bac system by insertion of the expression cassette into the polyhedrin gene locus of autographa californica nuclear polyhedrosis virus (acnpv). cytotoxinic effect caused by expression of barnase was observed in four mammalian cells after vaccmvbar infection dose-dependent analysis revealed that one of the cells hicam was the mos ... | 1998 | 18726228 |