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a role for pheromones in mate choice in a lekking sandfly.there is increasing evidence to suggest that pheromone-mediated communication may play an important role in mate choice in many insects. we investigated the possible role of pheromones in mate choice in the lekking sandfly, lutzomyia longipalpis, and explored whether males or females display any behavioural traits that predict patterns of male mating success. pairs of virgin males were introduced into a small mating chamber and allowed to interact for approximately 10 min prior to the introducti ...19989790700
morphological study of the larval spiracular system in eight lutzomyia species (diptera: psychodidae).the morphology of the spiracles of fourth instar larva in eight sandfly species were examined by light and scanning electron microscopy. species studied were: lutzomyia longipalpis (lutz & neiva), l. ovallesi (ortiz), l. youngi feliciangeli & murillo, l. evansi (nuñez-tovar), l. trinidadensis (newstead), l. migonei (frança), l. absonodonta feliciangeli, and l. venezuelensis (floch & abonnenc). in larvae of all eight species both thoracic and abdominal spiracles are located at the top of a globul ...20069698846
stomodaeal valve ultrastructure in the sandfly lutzomyia longipalpis (diptera: psychodidae).the stomodaeal valve and adjacent cardia region of the alimentary canal of the phlebotomine sandfly lutzomyia longipalpis have been studied using electron microscopy. with the scanning electron microscope (sem), numerous pit-like structures of different sizes were found in the surface of the valve of male and female flies. under the transmission electron microscope (tem), the cells adjacent to the pores were shown to have numerous mitochondria and to lack neural innervation. it is suggested that ...19989622365
sugar feeding and fluid destination control in the phlebotomine sandfly lutzomyia longipalpis (diptera: psychodidae).sandfly feeding behaviour and destination of coloured sugar meals in the gut of lutzomyia longipalpis were investigated with particular attention to the role of the crop. sandflies were able to ingest sugar from liquid drops, microcapillaries, a slice of pear and even sugar powder. in most cases the flies adopted a 'sugar feeding mode' with raised palps. as the fruit dried, flies of both sexes fed by piercing the tissue with the proboscis. all sugar-fed flies had a full crop plus a small amount ...19989513934
patterns of genetic variability in colonized strains of lutzomyia longipalpis (diptera: psychodidae) and its consequences.the genetic qualities of laboratory colonies of phlebotomine sand flies have not been compared with field specimens despite 1) probable genetic shifts due to the colonization process and 2) the problems associated with the extrapolation of experimental data derived from colonized organisms to field populations. the present study compared the genetic profiles of five laboratory colonies of geographic strains of the new world sand fly lutzomyia longipalpis, and contrasted them with field populatio ...19979288819
maxadilan, the vasodilator from sand flies, is a specific pituitary adenylate cyclase activating peptide type i receptor agonist.maxadilan is a potent vasodilator peptide isolated from salivary gland lysates of the sand fly lutzomyia longipalpis, a vector of leishmaniasis. the peptide aids the fly in obtaining blood from the skin of its vertebrate hosts but the mammalian receptor through which this insect ligand acts was unknown. we demonstrate that maxadilan is an agonist of the type i receptor for pituitary adenylate cyclase activating peptide, a neuropeptide with vascular activity. this surprising observation is a uniq ...19978995389
spectral sensitivity in the eyes of male and female lutzomyia longipalpis sandflies.using electroretinogram recordings, the response of lutzomyia longipalpis sandfly eyes to a range of wavelengths of light was measured, and spectral sensitivity determined. the eyes of both male and female adult sandflies were found to respond maximally to light in the ultraviolet region (at 340 nm) with a secondary peak in the blue-green-yellow region at 520 nm for females and 546 nm for males. the mann-whitney u test showed no significant differences between males and females at corresponding ...19968994140
effect of fluorescent powder on lutzomyia longipalpis (diptera: psychodidae) and a simple device for marking sand flies.a simple device for marking phlebotomine sand flies with fluorescent powders is described and tested; the design of the new device is characterized by separate compartments for sand flies and powder. the effect of fluorescent powder on survival and mobility of the sand fly lutzomyia longipalpis was compared using our device (method a) and a single-container method (method b). mortality within 1 h of powder application was negligible for method a (0.9%), but was 19.8% for method b; in addition, m ...19968827598
structural characterization and location of disulphide linkages of a potent vasodilatory peptide, recombinant maxadilan, by a multiple mass spectrometric approach.a multiple mass spectrometric strategy using fast-atom bombardment (fab) and matrix-assisted laser desorption/ionization (maldi) has been used to confirm the sequence and to locate the disulfide linkages of recombinant maxadilan (r-maxadilan) (average molecular mass 7422.5 da), a potent vasodilatory peptide from lutzomyia longipalpis. maldi measurements of intact r-maxadilan, its reduced form and its pyridylethylated form (p-maxadilan) indicated the presence of four cys residues without major po ...19968624417
the influence of sugars and amino acids on the blood-feeding behaviour, oviposition and longevity of laboratory colony of lutzomyia longipalpis (lutz & neiva, 1912) (diptera: psychodidae, phlebotominae).schneider's drosophila medium, a complex amino acid rich medium was tested alone and with seven different sugars for some aspects of the biology of lutzomyia longipalpis. statistically significant results were obtained when sucrose was used alone, indicating that among the sugars tested, this is still the most suitable and practical one for the maintenance of l. longipalpis colonies. however, the addition of schneider's medium to a pool of different sugars, was suggested to be related with the a ...20038731372
laboratory evaluation of chlorfluazuron against larval phlebotomine sand flies (diptera: psychodidae).laboratory experiments were conducted to evaluate the effectiveness of chlorfluazuron an insect growth-inhibitor against lutzomyia longipalpis (lutz & neiva). second- and third-instar l. longipalpis were exposed to four different doses (0.65, 0.75, 0.80, and 0.85 mg/ml) of chlorfluazuron added in the larval diet. a direct relationship between dose and larval mortality was observed. larval mortality was higher in younger larvae. in addition to larval mortality, reduced capacity of the adult femal ...19947962946
response of adult sandflies, lutzomyia longipalpis (diptera: psychodidae), to sticky traps baited with host odour and tested in the laboratory.the responses of adult lutzomyia longipalpis lutz and neiva to sticky traps baited with entrained volatiles from a hamster were assessed in the laboratory. in a two-choice test, significantly more females and males were caught on odour-baited sticky traps than on control traps, and only a few adults remained alive and untrapped at the end of the experiments. in contrast, significantly fewer flies of both sexes were caught in a series of tests where both traps were unbaited. twice as many males a ...19947979631
isolation of oviposition pheromone from the eggs of the sandfly lutzomyia longipalpis.semiochemical components of eggs of the sandfly lutzomyia longipalpis (diptera: psychodidae) were separated by high performance liquid chromatography. hplc fractions were examined quantitatively and qualitatively by gas chromatography (gc). a bioassay was used to determine the oviposition attraction of gravid l. longipalpis to each of the fractions separately and a peak responsible for the semiochemical activity was identified. gravid flies were placed in individual oviposition tubes to determin ...19948025318
the thigmotropic oviposition response of the sandfly lutzomyia longipalpis (diptera: psychodidae) to crevices.two experiments were carried out to investigate if lutzomyia longipalpis would be attracted and stimulated to lay eggs in crevices. in the first experiment, females were introduced into a chamber and offered the choice of oviposition in a test site consisting of artificial crevices, and an open control site. significantly more eggs were laid in the crevices of the test site than in the control site. in the second experiment, females were individually isolated in vials containing vertically place ...19921463365
laboratory observations on mating and leklike aggregations in lutzomyia longipalpis (diptera: psychodidae).the mating behavior of the sand fly, lutzomyia longipalpis, was studied under laboratory conditions. male sand flies landed on the bodies of anesthetized mice, where they exhibited leklike displays such as wing fanning, parading, and aggression. lek behavior was more frequent in older males, and dominance hierarchies were established. male sand flies at leks copulated more frequently than male sand flies at other locations in the cage. females mated by older males produced more eggs than did fem ...19921495025
the pupa of lutzomyia longipalpis (diptera: psychodidae--phlebotominae). 19911841247
gas-chromatographic analysis of lutzomyia longipalpis tergal pheromone gland extract.lutzomyia longipalpis is the vector of visceral leishmaniasis (vl) in south america. previous gas-chromatographic (gc) analyses of tergal gland extracts of lu. longipalpis have demonstrated two distinct pheromone types. these have been classified as a farnesene/homofarnesene type compound and a diterpene type compound. gc analysis of tergal gland extracts of lu. longipalpis from other areas of south america demonstrates that there may be other different pheromone producing forms of this sandfly. ...19911841219
chemical factors controlling oviposition of lutzomyia longipalpis (diptera: psychodidae).in choice chambers, lutzomyia longipalpis females were attracted and/or stimulated to lay eggs on sites containing hexane extracts of conspecific eggs, a result which supported previous findings on the presence of an oviposition pheromone on the eggs. gas chromatography analysis of hexane extracts of the eggs demonstrated the presence of several compounds, of which cholesterol and squalene were identified. cholesterol and squalene did not, however, induce an oviposition attraction and/or stimula ...19911841210
mating behaviour between different lines of lutzomyia longipalpis (diptera: psychodidae).homologous and heterologous crosses were made between laboratory-reared lutzomyia longipalpis. one line of insects originated from para state and the other from minas gerais. homologous matings showed that the two lines behaved differently before and during coition and that a mixture of the two behaviour patterns occurred in heterologous crossings.19911841205
[a new case of autochthonous visceral leishmaniasis in bolivia].a sixth autochthonous case of visceral leishmaniasis is reported in bolivia. it is also the fourth case detected in the yungas valley (department of la paz) confirming the long-term existence of the disease in this area where cases of canine leishmaniasis and natural infestation of the phlebotomine sandfly, lutzomyia longipalpis, were previously reported.19911665056
host-parasite relationships of ascogregarina chagasi (eugregarinorida, aseptatorina, lecudinidae) in lutzomyia longipalpis (diptera: psychodidae).the life cycle of ascogregarina chagasi in larvae and in adult female lutzomyia longipalpis was studied by light and electron microscopy. sporozoites and young gamonts were attached to the epithelial lining of the larval midgut via an osmiophilic contact zone. the mucron of young gamonts was bordered by an invaginated pellicular fold, and an electron-opaque vesicular structure was observed adjacent to it. sporozoites possessed an apical complex and were bound by a double membrane with underlying ...19911904054
response of the sandfly lutzomyia longipalpis to an oviposition pheromone associated with conspecific eggs.response of female lutzomyia longipalpis lutz & neiva (diptera: psychodidae) to an oviposition attractant and/or stimulant associated with conspecific eggs was investigated in the laboratory. females of two populations laid significantly higher mean number of eggs on sites with 160 eggs already present than on bare control sites. this response was lost when eggs placed on test sites were previously washed in organic solvents and distilled water. age of eggs placed on test sites, 1-6 days after b ...19911768905
histology and ultrastructure of pheromone secreting glands in males of the phlebotomine sandfly lutzomyia longipalpis.well-defined pheromone-producing glands are present on the third and fourth abdominal segments of male lutzomyia longipalpis. each gland consists of numerous large secretory cells. the individual cells have a complicated 'end-apparatus' and central reservoir which are connected directly to the exterior via a small chitinized duct. this is the first ultrastructural study of pheromone secreting cells of diptera.19902331176
response of female sandflies (lutzomyia longipalpis) to pheromone-baited sticky traps in the laboratory.response of virgin female lutzomyia longipalpis lutz & nevia (diptera: psychodidae), contained in a 60-cm3 net cage, to sticky traps was significantly greater when the traps were baited with extract of male pheromone glands. results confirm that the response of females to male pheromone does not necessarily depend upon the presence of a host.19902331175
[impact of the spraying of deltamethrin in a focus of leishmaniasis in bolivia].after one month of entomological observations to record pretreatment data, a sub andean village of yungas, bolivia (alt. 1500 m) was sprayed at the beginning of the rainy season (january 1987). houses were treated inside and outside with deltamethrin at 0.025 g/sq.m.; kennels, hen-houses and stacks of adobe were also sprayed in the same way. as a result of the treatment, lutzomyia longipalpis, the local vector of visceral leishmaniasis, disappeared from houses and animal shelters for 9 and 10 mo ...19892610530
an immune response-dependent mechanism for the vertical transmission of an entomopathogen.an exceptionally efficient mechanism for the vertical transmission of a parasitic gregarine is dependent on the insect host's immune response. gametocysts of ascogregarina chagasi on the genital accessory glands of adult female sand flies (lutzomyia longipalpis) become encapsulated through hemocyte-mediated immune reactions. oocysts of a. chagasi, ejected into the lumen of the glands owing to pressure exerted by this capsule, become glued to eggshells and are subsequently ingested by larvae. in ...19892759214
laboratory response of female lutzomyia longipalpis sandflies to a host and male pheromone source over distance.a nylon net cage (260 x 40 x 40 cm) provided with unidirectional air flow was used to demonstrate the response of female sandflies, lutzomyia longipalpis lutz & neiva (diptera: psychodidae) to a host and extract of male pheromone glands over a distance of 240 cm. females responded more rapidly and in greater numbers to the host if male pheromone was present. results suggest that one function of the pheromone may be to attract females from some distance to a common mating site, on or near the hos ...19892519666
susceptibility of lutzomyia longipalpis to deltamethrin. 20123271938
a practical method for mass breeding of sandflies in the laboratory: lutzomyia intermedia (lutz & neiva, 1912) and lutzomyia longipalpis (lutz & neiva, 1912) (diptera, psychodidae). 20033507576
development of a continuous cell line from the sand fly lutzomyia longipalpis (diptera: psychodidae), and its susceptibility to infection with arboviruses. 19836842527
factors affecting egg production of laboratory-bred lutzomyia longipalpis (diptera: psychodidae). 1979541814
the feeding habits of laboratory-bred lutzomyia longipalpis (diptera: psychodidae). 1978633293
the establishment, maintenance and productivity of a laboratory colony of lutzomyia longipalpis (diptera: psychodidae). 1977845881
proceedings: demonstration of giant chromosomes in the sandfly lutzomyia longipalpis (lutz and neiva, 1912). 19751216303
a laboratory culture of lutzomyia longipalpis. 19734801753
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