| accumulation and mobilization of storage lipids by rhodococcus opacus pd630 and rhodococcus ruber ncimb 40126. | the time course of the accumulation of triacylglycerols (tags) in rhodococcus opacus pd630 or of tags plus polyhydroxyalkanoates (pha) in rhodococcus ruber ncimb 40126 with gluconate or glucose as carbon source, respectively, was studied. in addition, we examined the mobilization of these storage compounds in the absence of a carbon source. r. opacus accumulated tags only after the exhaustion of ammonium in the medium, and, with a fixed concentration of the carbon source, the amounts of tags in ... | 2000 | 10968636 |
| distribution of alkb genes within n-alkane-degrading bacteria. | fifty-four bacterial strains belonging to 37 species were tested for their ability to assimilate short chain and/or medium chain liquid n-alkanes. a gene probe derived from the alkb gene of pseudomonas oleovorans atcc 29347 was utilized in hybridization experiments. results of southern hybridization of pcr-amplificates were compared with those of colony hybridization and dot blot hybridization. strongest signals were received only from gram-negative bacteria growing solely with short n-alkanes ( ... | 2000 | 10971768 |
| effects of prior feeding on pharmacokinetics and estimated bioavailability after oral administration of a single dose of microencapsulated erythromycin base in healthy foals. | to determine effects of prior feeding on pharmacokinetics and estimated bioavailability of orally administered microencapsulated erythromycin base (meb) in healthy foals. | 2000 | 10976728 |
| cloning and expression of the nitrile hydratase and amidase genes from bacillus sp. br449 into escherichia coli. | a moderate thermophile, bacillus sp. br449 was previously shown to exhibit a high level of nitrile hydratase (nhase) activity when growing on high levels of acrylonitrile at 55 degrees c. in this report, we describe the cloning of a 6.1 kb sali dna fragment encoding the nhase gene cluster of br449 into escherichia coli. nucleotide sequencing revealed six orfs encoding (in order), two unidentified putative proteins, amidase, nhase beta- and alpha-subunits and a small putative protein of 101 amino ... | 2000 | 10978771 |
| in vitro effects of sterculic acid on lipid biosynthesis in rhodococcus opacus strain pd630 and isolation of mutants defective in fatty acid desaturation. | the in vivo effects of sterculic acid methyl ester on triacylglycerol fatty acid composition in the oleaginous, hydrocarbon-degrading bacterium r. opacus strain pd630 was investigated. sterculic acid, a cyclopropene fatty acid and an inhibitor of the stearoyl-coa desaturase system, strongly inhibited the synthesis of monoenic fatty acids, of saturated fatty acids with more than 16 carbon atoms and of odd-numbered fatty acids when added to the culture medium. in addition, chemical mutagenesis and ... | 2000 | 10981688 |
| leafy gall formation is controlled by fasr, an arac-type regulatory gene in rhodococcus fascians. | rhodococcus fascians can interact with many plant species and induce the formation of either leafy galls or fasciations. to provoke symptoms, r. fascians strain d188 requires pathogenicity genes that are located on a linear plasmid, pfid188. the fas genes are essential for virulence and constitute an operon that encodes, among other functions, a cytokinin synthase gene. expression of the fas genes is induced by extracts of infected plant tissue only. we have isolated an arac-type regulatory gene ... | 2000 | 11004184 |
| the iron dependent regulatory protein ider (dtxr) of rhodococcus equi. | this paper reports the presence of an ider gene, which encodes an iron-dependent regulatory protein, in rhodococcus erythropolis and in the intracellular pathogen rhodococcus equi. the ider gene of the latter encoded a protein of 230 amino acids with a molecular mass of 25619. the alpha-helices forming the helix-turn-helix motif of the r. equi protein were identical to those of the dtxr protein of corynebacterium diphtheriae, which is an ider homologue. this indicates that the two proteins bind ... | 2000 | 11004391 |
| [growth of rhodococcus opacus on mixtures of monohalogenated benzenes and phenols]. | the growth of rhodococcus opacus gm-14 on mixtures of 2-chloro- and 2-bromophenol, of 4-chloro, 4-bromo-, and 4-iodophenol, and of chloro-, bromo-, and iodobenzenes was accompanied by consumption of the substrates and excretion of halogen ions to the medium. during the growth on monochlorophenols, the substrates were consumed sequentially in the following order: 4-chloro-, 3-chloro-, and then 2-chlorophenol. chlorine ions were excreted in a two-phase manner in amounts comprising 79% of the theor ... | 2000 | 11008684 |
| bacterial phosphating of mild (unalloyed) steel. | mild (unalloyed) steel electrodes were incubated in phosphate-buffered cultures of aerobic, biofilm-forming rhodococcus sp. strain c125 and pseudomonas putida mt2. a resulting surface reaction leading to the formation of a corrosion-inhibiting vivianite layer was accompanied by a characteristic electrochemical potential (e) curve. first, e increased slightly due to the interaction of phosphate with the iron oxides covering the steel surface. subsequently, e decreased rapidly and after 1 day reac ... | 2000 | 11010888 |
| microbial degradation of the multiply branched alkane 2,6,10,15,19, 23-hexamethyltetracosane (squalane) by mycobacterium fortuitum and mycobacterium ratisbonense. | among several bacterial species belonging to the general gordonia, mycobacterium, micromonospora, pseudomonas, and rhodococcus, only two mycobacterial isolates, mycobacterium fortuitum strain nf4 and the new isolate mycobacterium ratisbonense strain sd4, which was isolated from a sewage treatment plant, were capable of utilizing the multiply branched hydrocarbon squalane (2,6,10,15,19, 23-hexamethyltetracosane) and its analogous unsaturated hydrocarbon squalene as the sole carbon source for grow ... | 2000 | 11010899 |
| stable isotope incorporation triples the upper mass limit for determination of elemental composition by accurate mass measurement. | by comparing electrospray ionization fourier-transform ion cyclotron resonance (ft-icr) mass spectra and collision-induced dissociation (cid) ft-icr mass spectra of a phospholipid (851 da) extracted from natural abundance and 99% 13c bacterial growth media, we are able to reduce its number of possible elemental compositions (based on +/-10 ppm externally calibrated mass accuracy and biologically relevant compositional constraints) from 394 to 1. the basic idea is simply that the mass of a molecu ... | 2000 | 11014445 |
| immunohistochemical detection of virulence-associated rhodococcus equi antigens in pulmonary and intestinal lesions in horses. | two horses with rhodococcus equi infection were examined post mortem by an immunohistochemical method (peroxidase-antiperoxidase; pap) with a monoclonal antibody (mab 10g5) to the 15-17 kda antigen of r. equi. one of the horses was also examined bacteriologically, r. equi being isolated in culture. immunolabelling with this mab was marked and widespread. on the other hand, the immunohistochemical reactivity of infected macrophages with a polyclonal antibody specific for lysozyme was slight. thus ... | 2000 | 11032673 |
| hydrolysis of lysergamide to lysergic acid by rhodococcus equi a4. | from a mixture of lysergamide and its epimer isolysergamide, rhodococcus equi a4 containing amidase preferentially hydrolyzed lysergamide into lysergic acid. | 2001 | 11035188 |
| [rhodococcus equi brain abscess in a patient in hemodialysis]. | | 2000 | 11039267 |
| [a diagnostic medium for arcanobacterium haemolyticum and other bacterial species reacting with hemolytic synergism to the equi-factor of rhodococcus equi]. | colonies of arcanobacterium haemolyticum on common blood agar can be easily overlooked. therefore a diagnostic medium was developed, on which a. haemolyticum colonies produce a conspicuous zone of complete hemolysis. the medium under question is blood agar prepared from the columbia blood agar base and 5% washed sheep erythrocytes sensitised with equi factor (ef) of rhodococcus equi. optimally, 10 activity units (au) of ef per 1 ml were used. ef was titrated on a non-nutrient medium consisting o ... | 2000 | 11040494 |
| identification of corynebacterium bovis and other coryneforms isolated from bovine mammary glands. | bovine mastitis remains the most economically important disease in dairy cows. corynebacterium bovis, a lipid-requiring corynebacterium spp., is frequently isolated from the milk of infected mammary glands of dairy cows and is associated with reduced milk production. a total of 212 coryneform bacteria isolated from the milk of dairy cows were obtained from mastitis reference laboratories in the united states and canada. all isolates had been presumptively identified as corynebacterium bovis base ... | 2000 | 11049082 |
| relationships between colony morphotypes and oil tolerance in rhodococcus rhodochrous. | a mucoidal strain of rhodococcus rhodochrous was resistant to 10% (vol/vol) n-hexadecane, while its rough derivatives were sensitive. when the extracellular polysaccharide (eps) produced by the mucoidal strain was added to cultures of the rough strains, the rough strains gained resistance to n-hexadecane. thus, eps confer tolerance to n-hexadecane in members of the genus rhodococcus. | 2000 | 11055965 |
| [rhodococcus equi pneumonia in patients with aids]. | rhodococus equi infection is frequent in animals, but rare in humans. it usually appears as an opportunistic infection, occurring in clinical settings where a compromise of cellular immunity exists, such as in leukemia, neoplastic or transplanted patients. it is currently considered, as another opportunistic aids infection. sixty six cases of rhodococus equi pneumonia have been published until march 1996. the differential diagnosis includes tb and staphyloccocal pneumonia and is generally an inf ... | 2000 | 11059055 |
| clinical application of a polymerase chain reaction assay in the diagnosis of pneumonia caused by rhodococcus equi in a horse. | diagnosis of pneumonia caused by rhodococcus equi can be made more rapidly by use of a polymerase chain reaction (pcr) assay than by use of conventional bacteriologic culture techniques. use of a pcr assay aids in the differentiation between virulent and avirulent strains of r equi, and the assay may be used to identify r equi in feces and soil of breeding farms. | 2000 | 11061388 |
| biodegradation of a high molecular weight aliphatic ether--indications of an unusual biodegradation pathway. | an aliphatic ether (1-phytanyl-1-octadecanyl-ether) of high molecular weight was used as a sole carbon source in degradation experiments with different aerobic bacteria. the enriched culture b5, obtained from fuel contaminated soils, was able to degrade the substance for more than 90%. a culture of rhodococcus ruber was similarly effective. detailed investigation of the metabolites allowed us to characterize an unusual degradation pathway via a mid-chain oxidation mechanism ('internal oxidative ... | 1999 | 11068823 |
| [cavitating pneumonia in a patient infected with human immunodeficiency virus]. | | 2000 | 11075487 |
| dna sequence and comparison of virulence plasmids from rhodococcus equi atcc 33701 and 103. | the virulence plasmids of the equine virulent strains rhodococcus equi atcc 33701 and 103 were sequenced, and their genetic structure was analyzed. p33701 was 80,610 bp in length, and p103 was 1 bp shorter; their sequences were virtually identical. the plasmids contained 64 open reading frames (orfs), 22 of which were homologous with genes of known function and 3 of which were homologous with putative genes of unknown function in other species. putative functions were assigned to five orfs based ... | 2000 | 11083803 |
| search for virulence-associated antigens of rhodococcus equi in strains isolated from patients with acquired immunodeficiency syndrome. | rhodococcus equi (formerly corynebacterium equi) are known to be highly virulent, intermediate in virulence, or avirulent correlated with specific virulence-associated antigens identified immunochemically by different molecular weights. the association of virulence antigens with infection of aids patients by this organism has not been sufficiently evaluated in brazil or italy. the objective of the present study was to search for virulence-associated antigens of 15-to 17-kd and 20-kd in rhodococc ... | 1999 | 11084666 |
| crystal structure of the haloalkane dehalogenase from sphingomonas paucimobilis ut26. | the haloalkane dehalogenase from sphingomonas paucimobilis ut26 (linb) is the enzyme involved in the degradation of the important environmental pollutant gamma-hexachlorocyclohexane. the enzyme hydrolyzes a broad range of halogenated cyclic and aliphatic compounds. here, we present the 1.58 a crystal structure of linb and the 2.0 a structure of linb with 1,3-propanediol, a product of debromination of 1,3-dibromopropane, in the active site of the enzyme. the enzyme belongs to the alpha/beta hydro ... | 2000 | 11087355 |
| evaluation of nasotracheal aspiration as a diagnostic tool for rhodococcus equi pneumonia in foals. | the reliability of preparing bacteriological cultures from nasotracheal aspirates of foals routinely in order to diagnose r. equi pneumonia in foals was studied by isolating rhodococcus equi from specimens obtained from 96 foals by nasotracheal aspiration with a silicon catheter. results were compared with specimens obtained from 21 foals by transtracheal aspiration (percutaneous tracheal puncture). these 117 foals showed clinical signs of respiratory tract infection at sampling. r. equi was iso ... | 2000 | 11093633 |
| composition of soil microbial communities enriched on a mixture of aromatic hydrocarbons. | soil contaminated with c5+, which contained benzene (45%, wt/wt), dicyclopentadiene (dcpd) plus cyclopentadiene (together 20%), toluene (6%), styrene (3%), xylenes (2%), naphthalene (2%), and smaller quantities of other compounds, served as the source for isolation of 55 genomically distinct bacteria (standards). use of benzene as a substrate by these bacteria was most widespread (31 of 44 standards tested), followed by toluene (23 of 44), xylenes (14 of 44), styrene (10 of 44), and naphthalene ... | 2000 | 11097903 |
| isolation and characterization of corynebacterium ulcerans from cephalic implants in macaques. | to determine the prevalence of colonization by corynebacterium ulcerans, we cultured samples from the cephalic implant-skin margin and pharynx of 26 rhesus macaques and one pig-tailed macaque. all but one of the samples from the cephalic implants yielded a mixed population of bacteria. c. ulcerans grew from the cephalic implants in 56% and from the pharynx in 3% of the implanted animals. we screened nine of these isolates for diphtheria toxin (dt) and phospholipase d (pld). polymerase chain reac ... | 2000 | 11099137 |
| biodesulfurization and the upgrading of petroleum distillates. | biotechnology offers an alternative way to process fossil fuels. there have been several important advances in the elucidation of the mechanisms of biodesulfurization and the development of a biocatalytic desulfurization process. these include a detailed analysis of the rate and extent of desulfurization of real target molecules in a diesel matrix, the directed evolution of rate- and extent-limiting enzymes for better performance and the expression of the genes in alternative hosts. process inno ... | 2000 | 11102787 |
| purification, characterization and crystallization of enzymes for dibenzothiophene desulfurization. | dszc and dsza, dbt monooxygenase and dbt sulfone monooxygenase, respectively, involved in dibenzothiophene (dbt) desulfurization, were purified to homogeneity from rhodococcus erythropolis d-1. the two enzymes were crystallized and enzymologically characterized. we found a high activity of flavin reductase in the non-dbt-desulfurizing bacterium, paenibacillus polymyxa a-1, which is essential for dszc and a activities, and purified to homogeneity and characterized the enzyme. | 2000 | 11105249 |
| [study of the integral toxicity of aqueous media, polluted by petroleum and petroleum products using bacterial tests]. | a biotest kit was used to assess the integral toxicity level of aquatic medium contamination with petroleum and petroleum-based products. the integral toxicity dynamics was also monitored during biodegradation of petroleum and petroleum-based products by an association of petroleum-degrading strains including acinetobacter sp., mycobacterium flavescens, and rhodococcus sp. the following bacterial tests were used: the bioluminescence (bl) test based on photobacterium leiognathi; electro-orientati ... | 2000 | 11116909 |
| [creation and use of a liquid substance based on association of petroleum-oxidizing bacteria]. | an association of four bacterial strains with high oil-oxidizing and bioemulsifying activities, psychrophilicity, resistance to chemical pollutants, and lack of pathogenicity was selected from a collection of natural oil-oxidizing microorganisms. a new liquid preparation containing stabilizers and preservatives that maintain the cell viability and oil-oxidizing activity during long-term storage was developed. a field experiment in oil-polluted sod-podzol and clay sand soils demonstrated that thi ... | 2000 | 11116911 |
| rhodococcus equi and hiv-1 infection in uganda. | to describe three cases of rhodococcus equi infection in a cohort of hiv-1 infected adults in entebbe, uganda and to compare this to the rates and presentation of tuberculosis in this cohort. | 2000 | 11120609 |
| salt-tolerant phenol-degrading microorganisms isolated from amazonian soil samples. | two phenol-degrading microorganisms were isolated from amazonian rain forest soil samples after enrichment in the presence of phenol and a high salt concentration. the yeast candida tropicalis and the bacterium alcaligenes faecoalis were identified using several techniques, including staining, morphological observation and biochemical tests, fatty acid profiles and 16s/18s rrna sequencing. both isolates, a. faecalis and c. tropicalis, were used in phenol degradation assays, with rhodococcus eryt ... | 2000 | 11131025 |
| resolution of alcohols by cholesterol oxidase fromrrhodococcus erythropolis: lack of enantiospecificity for the steroids. | | 2001 | 11135412 |
| application of solid-phase extraction to the analysis of the isomers generated in biodesulfurization against methylated dibenzothiophenes. | a solid-phase extraction (spe) technique was applied to analyze and characterize the biodesulfurization reactions against asymmetrically methylated dibenzothiophenes (mdbts) such as 1-, 2-, 3- and 4-methyldibenzothiophenes present in fossil fuels. recently, we found that these mdbts are efficiently degraded by the bacterial strain, rhodococcus erythropolis ka2-5-1. separation and concentration of the microbial desulfurization products from each of the mdbts could be carried out with high efficie ... | 2000 | 11153941 |
| rhodococcus pyridinivorans sp. nov., a pyridine-degrading bacterium. | the taxonomic position of a bacterial strain (pdb9t) that is capable of degrading pyridine was clarified by a polyphasic taxonomic approach using phenotypic, chemotaxonomic and genetic methods. the cells, which are rods and branched filaments during the early growth phase, fragment into short rods or cocci, thereby completing the growth cycle. strain pdb9t was found to have a cell wall of chemotype iv, mk-8(h2) as the predominant menaquinone, mycolic acids with 36-46 carbon atoms and c16:0' c18: ... | 2000 | 11155994 |
| genetic and biochemical characterization of a novel monoterpene epsilon-lactone hydrolase from rhodococcus erythropolis dcl14. | a monoterpene epsilon-lactone hydrolase (mlh) from rhodococcus erythropolis dcl14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol, was purified to apparent homogeneity. it is a monomeric enzyme of 31 kda that is active with (4r)-4-isopropenyl-7-methyl-2-oxo-oxepanone and (6r)-6-isopropenyl-3-methyl-2-oxo-oxepanone, lactones derived from (4r)-dihydrocarvone, and 7-isopropyl-4-methyl-2-oxo-oxepanone, the ... | 2001 | 11157238 |
| virulence plasmid of rhodococcus equi contains inducible gene family encoding secreted proteins. | rhodococcus equi causes severe pyogranulomatous pneumonia in foals. this facultative intracellular pathogen produces similar lesions in immunocompromised humans, particularly in aids patients. virulent strains of r. equi bear a large plasmid that is required for intracellular survival within macrophages and for virulence in foals and mice. only two plasmid-encoded proteins have been described previously; a 15- to 17-kda surface protein designated virulence-associated protein a (vapa) and an anti ... | 2001 | 11159951 |
| comparative specificities of two evolutionarily divergent hydrolases involved in microbial degradation of polychlorinated biphenyls. | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (hopda) hydrolase (bphd) is a key determinant in the aerobic transformation of polychlorinated biphenyls (pcbs) by burkholderia sp. strain lb400 (s. y. k. seah, g. labbé, s. nerdinger, m. johnson, v. snieckus, and l. d. eltis, j. biol. chem. 275:15701-15708, 2000). to determine whether this is also true in divergent biphenyl degraders, the homologous hydrolase of rhodococcus globerulus p6, bphd(p6), was hyperexpressed, purified to apparent homogeneity, a ... | 2001 | 11160080 |
| structure-activity relationship of mycoloyl glycolipids derived from rhodococcus sp. 4306. | novel mycoloyl glycolipids with short carbon chains were isolated and purified from rhodococcus sp. 4306, a soil origin of actinomycetales. their chemical structures were identified as trehalose 6,6'-dimycolate (tdm), trehalose 6-monomycolate, glucose 6-monomycolate, mannose 6-monomycolate and fructose 6-monomycolate. the length of carbon chains and number of double bonds of mycolic acids were c(34), c(36)and c(38)saturated, monoenoic and dienoic molecular species, which were much shorter than t ... | 2001 | 11162189 |
| virulence-associated protein characterisation of rhodococcus equi isolated from bovine lymph nodes. | rhodococcus equi has a low pathogenicity in cattle, but it occasionally causes lymph node granulomas, which are detected at abattoir post mortem inspection, and must be distinguished from tuberculous granulomas. lymph node lesions were detected in 6719 cattle, from a total of 3,263,622 cattle examined post mortem in abattoirs, in the republic of ireland, during 1997 and 1998. histological examination was performed on all lesions, principally for the purpose of identifying animals with tuberculos ... | 2001 | 11165066 |
| consecutive bacillary angiomatosis and rhodococcus equi bacteremia during acute leukemia: zoonoses may cause fever in neutropenic patients. | | 2000 | 11168145 |
| rhodococcus equi and cytomegalovirus pneumonia in a renal transplant patient: diagnosis by fine-needle aspiration biopsy. | rhodococcus equi is a common cause of pneumonia in animals. human infection is rare. increasing number of cases are being reported in immunosuppressed individuals mostly associated with hiv infection, but also in solid organ transplant recipients and leukemia/lymphoma patients. we report on an adult male who developed pneumonia and gastroenteritis 4 mo after receiving a renal transplant. ct scan of the lungs showed a dominant 2.5-cm upper lobe lung mass and smaller bilateral nodules. he underwen ... | 2001 | 11169894 |
| rhodococcus equi infections in immunocompetent hosts: case report and review. | rhodococcus equi is an unusual cause of infection in humans. infection in immunocompetent patients is extremely rare-only 19 cases in immunocompetent hosts have been reported. localized infections represent nearly 50% of reported cases. pulmonary infections account for only 42% of infections in immunocompetent hosts, compared with 84% of infections in immunocompromised hosts. the mortality rate among immunocompetent patients is approximately 11%, compared with rates of 50%-55% among human immuno ... | 2001 | 11170969 |
| resident aerobic microbiota of the adult human nasal cavity. | recent evidence strongly suggests that the microbiota of the nasal cavity plays a crucial role in determining the reaction patterns of the mucosal and systemic immune system. however, little is known about the normal microbiota of the nasal cavity. the purpose of this study was to determine the microbiota in different parts of the nasal cavity and to develop and evaluate methods for this purpose. samples were collected from 10 healthy adults by nasal washes and by swabbing of the mucosa through ... | 2000 | 11200821 |
| the role of apoptosis in normal ontogenesis and solid human neoplasms. | mammalian cells are capable of committing "active suicide" or apoptosis in response to specialized pathological mechanisms employing a phylogenetically developed intrinsic program of death, triggered by signal transduction through specific receptors. changes in cellular structure such as: 1) condensation of the nuclear (chromatin) and cytoplasmic structures (especially the mitochondria); 2) blebbing of the cell membrane; 3) characteristic swelling of the endoplasmic reticulum; and 4) fragmentati ... | 2000 | 11204498 |
| de novo cortical cell division triggered by the phytopathogen rhodococcus fascians in tobacco. | plant growth, development, and morphology can be affected by several environmental stimuli and by specific interactions with phytopathogens. in many cases, plants respond to pathogenic stimuli by adapting their hormone levels. here, the interaction between the phytopathogen rhodococcus fascians and one of its host plants, tobacco, was analyzed phenotypically and molecularly. to elucidate the basis of the cell division modulation and shoot primordia initiation caused by r. fascians, tobacco plant ... | 2001 | 11204782 |
| a taxonomic note on the authorship and date of valid publication of rhodococcus sputi. | authorship of the name rhodococcus sputi is variously attributed to tsukamura 1978 or tsukamura and yano 1985. dna-dna binding data indicate that this species and rhodococcus obuensis tsukamura 1983 and rhodococcus chubuensis tsukamura 1983 are subjective (heterotypic) synonyms. although these organisms have been placed in the genus gordonia as gordonia sputi, the correct name of the taxon created by unification of these three species is directly affected by the date of valid publication of thes ... | 2001 | 11211266 |
| a novel assay method for glycosphingolipid deacylase by enzyme-linked immunochemical detection of lysoglycosphingolipid. | lysoglycosphingolipids consist of a sphingoid long-chain base and monosaccharide or complex sugar, and they lack the fatty acyl group present in native glycosphingolipids. less than 1 pmol of lyso-forssman glycolipid and lysoganglioside gm1 were detected on a thin-layer chromatogram by an enzyme-linked immunochemical coloration method with anti-forssman glycolipid antibody (fom-1) and cholera toxin b subunit, respectively. each spot between 1 and 100 pmol lyso-forssman glycolipid was immunostain ... | 2001 | 11214737 |
| enhancing desulphurization by engineering a flavin reductase-encoding gene cassette in recombinant biocatalysts. | biological desulphurization of petroleum feedstocks and products may offer an attractive alternative to reduce sulphur oxide emissions that cause serious environmental pollution. dibenzothiophene (dbt) desulphurization via the dsz pathway of rhodococcus erythropolis igts8 is an energetically expensive process that consumes reducing equivalents. we have shown in this work that the hpac oxidoreductase from escherichia coli w is able to supply the required fmnh2 to the dsz monooxygenases. the cloni ... | 2000 | 11214801 |
| bacterial sources and sinks of isoprene, a reactive atmospheric hydrocarbon. | | 2000 | 11220299 |
| analysis of bacterial community structure in sulfurous-oil-containing soils and detection of species carrying dibenzothiophene desulfurization (dsz) genes. | the selective effects of sulfur-containing hydrocarbons, with respect to changes in bacterial community structure and selection of desulfurizing organisms and genes, were studied in soil. samples taken from a polluted field soil (a) along a concentration gradient of sulfurous oil and from soil microcosms treated with dibenzothiophene (dbt)-containing petroleum (fsl soil) were analyzed. analyses included plate counts of total bacteria and of dbt utilizers, molecular community profiling via soil d ... | 2001 | 11229891 |
| purification, characterization, and overexpression of flavin reductase involved in dibenzothiophene desulfurization by rhodococcus erythropolis d-1. | the dibenzothiophene (dbt)-desulfurizing bacterium, rhodococcus erythropolis d-1, removes sulfur from dbt to form 2-hydroxybiphenyl using four enzymes, dszc, dsza, dszb, and flavin reductase. in this study, we purified and characterized the flavin reductase from r. erythropolis d-1 grown in a medium containing dbt as the sole source of sulfur. it is conceivable that the enzyme is essential for two monooxygenase (dszc and dsza) reactions in vivo. the purified flavin reductase contains no chromoge ... | 2001 | 11229908 |
| detection of genes for alkane and naphthalene catabolism in rhodococcus sp. strain 1bn. | rhodococcus sp. 1bn was isolated from a contaminated site and showed various biodegradative capabilities. besides naphthalene, strain 1bn degraded medium- (c6) and long-chain alkanes (c16-c28), benzene and toluene, alone or when the hydrocarbons were mixed in equal proportions. the nucleotide sequence of an alk polymerase chain reaction (pcr) fragment revealed a 59% nucleotide homology to the pseudomonas oleovorans alkb gene. the nar fragments were highly homologous to genes coding for large and ... | 2000 | 11233165 |
| [cavitated pulmonary condensation by rhodococcus equi, in an hiv seropositive individual]. | the first clinical case described in portugal of a human infection by rhodococcus equi in a 35 year-old hiv1 seropositive i.v. drug user is presented. the patient was admitted to hospital due to clinical and radiological suspicion of tuberculosis. while in hospital, a rhodococcus equi cavitated lung infection was diagnosed. by applying a triple antibiotic therapy with erythromycin, rifampin and teicoplanin along with surgical excision of the lower lobe of the right lung, we were able to clinical ... | 2000 | 11234501 |
| [causes of para-allergy to tuberculin]. | experiments on 56 rabbits infected with microorganisms of the genera mycobacterium, nocardia and rhodococcus revealed that in response to the action of different antigens used t and b systems of immunity induced synthesis of antibodies reactive with nonspecific antigens (mycobacterial antigens, tuberculin). in some cases the statistically significant correlation between the dynamics of blast transformation and the specific lysis of lymphocytes in animals with nocardia and rhodococcus infections ... | 2001 | 11236505 |
| chronic scalp wound infection due to rhodococcus equi in an immunocompetent patient. | a case of chronic scalp wound infection due to rhodococcus equi in an immunocompetent individual following heavily contaminated traumatic injury was successfully treated by combining antibiotics and surgery. the agents used were erythromycin and rifampicin. | 2001 | 11243757 |
| isolation of virulent rhodococcus equi from native japanese horses. | r. equi was isolated from soil samples obtained from the environment of seven native japanese horse breeds (hokkaido, kiso, noma, misaki, tokara, miyako and yonaguni) and from fecal samples collected from three native horse breeds (hokkaido, kiso and misaki). virulent r. equi at various levels (ranging from 0.5 to 12.9%) was isolated from the feces or soil environment of hokkaido, kiso and misaki horses. isolates were investigated both for the presence of 15- to 17-kda antigens (virulence-associ ... | 2001 | 11247045 |
| the fas locus of the phytopathogen rhodococcus fascians affects mitosis of tobacco by-2 cells. | the effect of rhodococcus fascians, the causal agent of leafy gall disease, on the mitotic behavior of synchronized tobacco bright yellow-2 (by-2) cells was investigated. incubation of aphidicolin-synchronized by-2 cells with r. fascians cells specifically resulted in a broader mitotic index peak, an effect that was linked to an intact and expressed fas virulence locus. the obtained results pointed towards an effect of r. fascians on the prophase of mitosis. the relevance of these results to the ... | 2001 | 11248250 |
| the bactericidal activities of hmr 3004, hmr 3647 and erythromycin against gram-positive bacilli and development of resistance. | the bactericidal activities of two new ketolides, hmr 3004 and hmr 3647, and the potential to develop resistance to these two antibiotics were studied in gram-positive bacilli. as judged by time-kill kinetics both ketolides were mostly bacteriostatic, being bactericidal against only highly susceptible isolates of corynebacterium striatum (two isolates) and corynebacterium minutissimum (one isolate). spontaneous resistant mutants were detected in seven of 30 strains tested, mainly in rhodococcus ... | 1999 | 11252337 |
| the desymmetrization of bicyclic beta -diketones by an enzymatic retro-claisen reaction. a new reaction of the crotonase superfamily. | the enzyme 6-oxocamphor hydrolase, which catalyzes the desymmetrization of 6-oxocamphor to yield (2r,4s)-alpha-campholinic acid, has been purified with a factor of 35.7 from a wild type strain of rhodococcus sp. ncimb 9784 grown on (1r)-(+)-camphor as the sole carbon source. the enzyme has a subunit molecular mass of 28,488 da by electrospray mass spectrometry and a native molecular mass of approximately 83,000 da indicating that the active protein is trimeric. the specific activity was determin ... | 2001 | 11278926 |
| long-range (1)h-(15)n heteronuclear shift correlation at natural abundance: a tool to study benzothiazole biodegradation by two rhodococcus strains. | the biodegradation of benzothiazole and 2-hydroxybenzothiazole by two strains of rhodococcus was monitored by reversed phase high-pressure liquid chromatography and by (1)h nuclear magnetic resonance (nmr). both xenobiotics were biotransformed into a hydroxylated derivative of 2-hydroxybenzothiazole by these two strains. the chemical structure of this metabolite was determined by a new nmr methodology: long-range (1)h-(15)n heteronuclear shift correlation without any previous (15)n enrichment of ... | 2001 | 11282584 |
| succession of phenotypic, genotypic, and metabolic community characteristics during in vitro bioslurry treatment of polycyclic aromatic hydrocarbon-contaminated sediments. | dredged harbor sediment contaminated with polycyclic aromatic hydrocarbons (pahs) was removed from the milwaukee confined disposal facility and examined for in situ biodegradative capacity. molecular techniques were used to determine the successional characteristics of the indigenous microbiota during a 4-month bioslurry evaluation. ester-linked phospholipid fatty acids (plfa), multiplex pcr of targeted genes, and radiorespirometry techniques were used to define in situ microbial phenotypic, gen ... | 2001 | 11282603 |
| deep desulfurization of extensively hydrodesulfurized middle distillate oil by rhodococcus sp. strain ecrd-1. | dibenzothiophene (dbt), and in particular substituted dbts, are resistant to hydrodesulfurization (hds) and can persist in fuels even after aggressive hds treatment. treatment by rhodococcus sp. strain ecrd-1 of a middle distillate oil whose sulfur content was virtually all substituted dbts produced extensive desulfurization and a sulfur level of 56 ppm. | 2001 | 11282654 |
| comparison of nucleic acid amplification, serology, and microbiologic culture for diagnosis of rhodococcus equi pneumonia in foals. | recently, a technique was described for amplification of rhodococcus equi-specific chromosomal and vapa dna from blood and tracheal wash fluids. it was hypothesized that this technique would be more sensitive than standard culture techniques or serology for diagnosis of r. equi pneumonia in foals. tracheal wash fluid, nasal swabs, whole blood samples, and serum samples from 56 foals with pneumonia were analyzed. final clinical diagnosis was determined by the attending clinician on the basis of f ... | 2001 | 11283043 |
| tsukamurella strandjordae sp. nov., a proposed new species causing sepsis. | we have isolated a gram-positive, weakly acid-alcohol-fast, irregular rod-shaped bacterium from cultures of blood from a 5-year-old girl with acute myelogenous leukemia. this isolate was compared with 14 other strains including reference strains of tsukamurella species by a polyphasic approach based on physiological and biochemical properties, whole-cell short-chain fatty acid and mycolic acid analyses, dna-dna hybridization, and sequencing of the 16s rrna gene. this isolate represents a new tax ... | 2001 | 11283073 |
| b-cell epitope mapping of the vapa protein of rhodococcus equi: implications for early detection of r. equi disease in foals. | linear b-cell epitopes of the rhodococcus equi virulence-associated protein (vapa) were mapped using a synthetic peptide bank in this study. the peptides were screened in an enzyme-linked immunosorbent assay (elisa) with a total of 70 sera from foals with current r. equi disease (51 sera), as well as from foals that had either recovered from r. equi infection 10 months previously (3 sera) or that had no known history of r. equi disease (16 sera). an epitope with the sequence nlqkdepngra was iden ... | 2001 | 11283104 |
| dna shuffling method for generating highly recombined genes and evolved enzymes. | we introduce a method of in vitro recombination or "dna shuffling" to generate libraries of evolved enzymes. the approach relies on the ordering, trimming, and joining of randomly cleaved parental dna fragments annealed to a transient polynucleotide scaffold. we generated chimeric libraries averaging 14.0 crossovers per gene, a several-fold higher level of recombination than observed for other methods. we also observed an unprecedented four crossovers per gene in regions of 10 or fewer bases of ... | 2001 | 11283594 |
| fite stain positivity in rhodococcus equi: yet another acid-fast organism in respiratory cytology--a case report. | rhodococcus equi is an aerobic gram-positive and acid-fast coccobacillus that may cause cavitary pneumonia in immunocompromised hosts such as hiv-infected patients. numerous grocott's methenamine silver (gms)-positive organisms were initially noted on the direct smear; a minor number of acid-fast organisms were seen in the thin-prep slide. since the abundant mucous material with the attached organisms seen in conventional smears may be lost in liquid-based preparations, more sensitive stains suc ... | 2001 | 11285619 |
| fe-type nitrile hydratase. | the characteristic features of fe-type nitrile hydratase (nhase) from rhodococcus sp. n-771 are described. through the biochemical analyses, we have found that nitric oxide (no) regulates the photoreactivity of this enzyme by association with the non-heme iron center and photoinduced dissociation from it. the regulation is realized by a unique structure of the catalytic non-heme iron center composed of post-translationally modified cysteine-sulfinic (cys-so2h) and -sulfenic acids (cys-soh). to u ... | 2001 | 11293544 |
| microbial diversity in hot synthetic compost as revealed by pcr-amplified rrna sequences from cultivated isolates and extracted dna. | high-temperature (>/=60 degrees c) synthetic food waste compost was examined by cultivation-dependent and -independent methods to determine predominant microbial populations. fluorescent direct counts totaled 6.4 (+/-2.5)x10(10) cells gdw(-1) in a freeze-dried 74 degrees c compost sample, while plate counts for thermophilic heterotrophic aerobes averaged 2.6 (+/-1.0)x10(8) cfu gdw(-1). a pre-lysis cell fractionation method was developed to obtain community dna and a suite of 16s and 18s rdna-tar ... | 2001 | 11295460 |
| vitamin requirements of hydrocarbon-utilizing soil bacteria. | the numbers of oil-utilizing bacteria in several samples of clean and oil-polluted soils counted on vitamin-containing media were severalfold higher than the numbers counted on vitamin-free media. colonies that grew on a medium containing a vitamin mixture were tested for growth on the same medium lacking any vitamins. more than 90% of the total colonies failed to grow. the remaining 10% grew, yet their growth was enhanced, when vitamins were added. the predominant oil-utilizing bacteria in one ... | 2001 | 11297361 |
| improvement of desulfurization activity in rhodococcus erythropolis ka2-5-1 by genetic engineering. | rhodococcus erythropolis ka2-5-1 can desulfurize dibenzothiophene (dbt) into 2-hydroxybiphenyl. a cryptic plasmid, prc4, which was derived from r. rhodochrous ifo3338, was combined with an escherichia coli vector to construct an e. coli-rhodococcus shuttle vector. the complete nucleotide sequence of 2582-bp prc4 was analyzed. based on the characteristics of its putative replication genes, prc4 was assigned to the family of pal5000-related replicons. the desulfurization gene cluster, dszabc, and ... | 2001 | 11302154 |
| kinetic analysis of microbial desulfurization of model and light gas oils containing multiple alkyl dibenzothiophenes. | the reaction mechanism of biodesulfurization was investigated using whole cells of rhodococcus erythropolis ka2-5-1, which have the ability to convert dibenzothiophene (dbt) into 2-hydroxybiphenyl. the desulfurization patterns of alkyl dbts were represented by the michaeis-menten equation. the values of rate constants, the limiting maximal velocity (vmax) and michaelis constant (km), for desulfurization of alkyl dbts were calculated. the relative desulfurization activities of various alkyl dbts ... | 2001 | 11302162 |
| [clusterization of halophilic and halotolerant eubacteria using whole-cell protein electrophoresis data]. | total cell proteins of the nineteen halophilic and halotolerant eubacteria isolated from marine sediments and highly mineralized formation waters of oil fields were investigated by sds gel electrophoresis. the microorganisms studied, phenotypically identified as belonging to the genera dietzia, rhodococcus, staphylococcus, cytophaga, brevibacterium, and archangium, were found to form clearly distinguishable clusters (20-30% similarity at the generic level) on the dendrogram derived from electrop ... | 2000 | 11315673 |
| characterization of the 450-kb linear plasmid in a polychlorinated biphenyl degrader, rhodococcus sp. strain rha1. | a strong polychlorinated biphenyl (pcb) degrader, rhodococcus sp. strain rha1, has diverse biphenyl/pcb degradative genes and harbors huge linear plasmids, including prhl1 (1,100 kb), prhl2 (450 kb), and prhl3 (330 kb). the diverse degradative genes are distributed mainly on the prhl1 and prhl2 plasmids. in this study, the structural and functional characteristics of prhl2 were determined. we constructed a physical map of prhl2, and the degradative enzyme genes, including bphb2, etbd2, etbc, bph ... | 2001 | 11319076 |
| epidemiology of rhodococcus equi strains on thoroughbred horse farms. | pulsed-field gel electrophoresis of restriction endonuclease-digested genomic dna from a large collection of clinical isolates of rhodococcus equi, an important pathogen of foals, was used to compare strain distribution between farms and over time. forty-four strains were found among 209 isolates, with 5 of these accounting for over half the isolates and the 22 strains isolated more than once accounting for 90% of the isolates. the average genotypic diversity on each farm and in each year was fo ... | 2001 | 11319096 |
| purification and characterization of the enantioselective nitrile hydratase from rhodococcus equi a4. | the nitrile hydratase from rhodococcus equi a4 consisted of two kinds of subunits which slightly differed in molecular weight (both approximately 25 kda) and showed a significant similarity in the n-terminal amino acid sequences to those of the nitrile hydratase from rhodococcus sp. n-774. the enzyme preferentially hydrated the s-isomers of racemic 2-(2-, 4-methoxyphenyl)propionitrile, 2-(4-chlorophenyl)propionitrile and 2-(6-methoxynaphthyl)propionitrile (naproxennitrile) with e-values of 5-15. ... | 2001 | 11330707 |
| the plant pathogen rhodococcus fascians colonizes the exterior and interior of the aerial parts of plants. | rhodococcus fascians is a plant-pathogenic bacterium that causes malformations on aerial plant parts, whereby leafy galls occur at axillary meristems. the colonization behavior on nicotiana tabacum and arabidopsis thaliana plants was examined. independent of the infection methods, r. fascians extensively colonized the plant surface where the bacteria were surrounded by a slime layer. r. fascians caused the collapse of epidermal cells and penetrated intercellularly into the plant tissues. the ons ... | 2001 | 11332724 |
| bacterial symbiosis and paratransgenic control of vector-borne chagas disease. | the triatomine vectors of chagas disease are obligate haematophagous insects, feeding on vertebrate blood throughout their entire developmental cycle. as a result of obtaining their nutrition from a single food source, their diet is devoid of certain vitamins and nutrients. consequently, these insects harbour populations of bacterial symbionts within their intestinal tract, which provide the required nutrients that are lacking from their diet. we have isolated and characterised symbiont cultures ... | 2001 | 11334952 |
| kupffer cells of cirrhotic rat livers sensitize colon cancer cells to fas-mediated apoptosis. | metastasis of colorectal carcinomas rarely occurs in cirrhotic livers. our study investigated the influence of activated kupffer cells from cirrhotic rat livers on hepatic colonization and fasr-mediated apoptosis of colon cancer cells. a rat colon cancer cell line, rcn-9, was used to inoculate rat livers. treatment with conditioned media of kupffer cells isolated from ccl(4)-induced cirrhotic rat livers (cirrhotic kcm) significantly reduced the incidence of hepatic colonization of rcn-9 cells. i ... | 2001 | 11336480 |
| [bacteria--degraders of polycyclic aromatic hydrocarbons, isolated from soil and bottom sediments in salt-mining areas]. | fifteen bacterial strains capable of utilizing naphthalene, phenanthrene, and biphenyl as the sole sources of carbon and energy were isolated from soils and bottom sediments contaminated with waste products generated by chemical and salt producing plants. based on cultural, morphological, and chemotaxonomic characteristics, ten of these strains were identified as belonging to the genera rhodococcus, arthrobacter, bacillus, and pseudomonas. all ten strains were found to be halotolerant bacteria c ... | 2001 | 11338839 |
| long-term repeated biodesulfurization by immobilized rhodococcus erythropolis ka2-5-1 cells. | in this study, biodesulfurization (bds) was carried out using immobilized rhodococcus erythropolis ka2-5-1 in n-tetradecane containing dibenzothiophene (dbt) as a model oil (n-tetradecane/immobilized cell biphasic system). the cells were immobilized by entrapping them with calcium alginate, agar, photo-crosslinkable resin prepolymers (ent-4000 and entp-4000), and urethane prepolymers (pu-3 and pu-6); and it was found that ent-4000-immobilized cells had the highest dbt desulfurization activity in ... | 2001 | 11341322 |
| protective effect of nocardia opaca lysozyme digest in experimental murine candida albicans infections. | candida albicans, as an opportunistic pathogen, causes therapeutic problems in immunocompetent individuals and frequently it initiates severe infections in immunocompromised hosts. the application of a lysozyme digest preparation from the cell walls of nocardia opaca (nocardia lysozyme digest; nld), recently classified as rhodococcus opacus, has a protective effect in intravenous (i.v.) c. albicans infections in inbred icr mice which have normal complement production. it also significantly reduc ... | 2001 | 11346272 |
| development of a rhodococcus recombinant strain for degradation of products from anaerobic dechlorination of pcbs. | the gram-positive bacterium rhodococcus sp. strain rha1, naturally containing the biphenyl pathway, was electroporated with a broad host range plasmid containing the 4-chlorobenzoate (4-cba) degradation operon (fcb) isolated from arthrobacter globiformis strain kzt1. the recombinant strain grew in medium containing 4-cba and 4-chlorobiphenyl (4-cb) as the only source of carbon, with stoichiometric release of chloride and a molar growth yield on 4-cb that suggested utilization of both biphenyl ri ... | 2001 | 11349275 |
| influence of hexaconazole, carbofuran and ethion on soil microflora and dehydrogenase activities in soil and intact cell. | total microbial count was highly affected (up to 61% at 1000 micrograms level) in presence of hexaconazole and persisted up to 21 days. bacteria were more susceptible than actinomycetes. carbofuran and ethion were moderately toxic to soil microflora. inhibitory effects of all the three pesticides gradually decreased after 21 days as was evident by increase in total microbial count except in carbofuran. gdh activity in soil was also affected initially (up to 14 days) by all the three pesticides ( ... | 2001 | 11349536 |
| photo quiz. disseminated rhodocuccus equi infection. | | 2001 | 11349643 |
| immunity to rhodococcus equi: antigen-specific recall responses in the lungs of adult horses. | rhodococcal pneumonia is an important disease of young horses that is not seen in immunocompetent adults. since all foals are normally exposed to rhodococcus equi in their environment, we hypothesized that most develop protective immune responses. furthermore, these antigen-specific responses were hypothesized to operate throughout adult life to prevent rhodococcal pneumonia. a better understanding of the mechanisms of immune clearance in adult horses would help define the requirements for an ef ... | 2001 | 11356253 |
| [assimilation of propane and properties of propan monooxygenase from rhodococcus erythropolis 3/89]. | the ability of propane-assimilating microorganisms of the genus rhodococcus to utilize metabolites of the terminal and subterminal pathways of propane oxidation was studied. propane monooxygenase of rhodococcus erythropolis 3/89 was shown to be the an inducible enzyme catalyzing epoxidation and hydroxylation of organic compounds. the optimum conditions for epoxidation of gaseous and liquid alkenes and hydroxylation of aromatic carbohydrates were found. | 2001 | 11357423 |
| dehalogenation, denitration, dehydroxylation, and angular attack on substituted biphenyls and related compounds by a biphenyl dioxygenase. | the attack by the bph-encoded biphenyl dioxygenase of burkholderia sp. strain lb400 on a number of symmetrical ortho-substituted biphenyls or quasi ortho-substituted biphenyl analogues has been investigated. 2,2'-difluoro-, 2,2'-dibromo-, 2,2'-dinitro-, and 2,2'-dihydroxybiphenyl were accepted as substrates. dioxygenation of all of these compounds showed a strong preference for the semisubstituted pair of vicinal ortho and meta carbons, leading to the formation of 2'-substituted 2,3-dihydroxybip ... | 2001 | 11371517 |
| cloning and expression of the benzoate dioxygenase genes from rhodococcus sp. strain 19070. | the bopxyz genes from the gram-positive bacterium rhodococcus sp. strain 19070 encode a broad-substrate-specific benzoate dioxygenase. expression of the bopxy terminal oxygenase enabled escherichia coli to convert benzoate or anthranilate (2-aminobenzoate) to a nonaromatic cis-diol or catechol, respectively. this expression system also rapidly transformed m-toluate (3-methylbenzoate) to an unidentified product. in contrast, 2-chlorobenzoate was not a good substrate. the bopxyz dioxygenase was ho ... | 2001 | 11375157 |
| complete denitration of nitroglycerin by bacteria isolated from a washwater soakaway. | four axenic bacterial species capable of biodegrading nitroglycerin (glycerol trinitrate [gtn]) were isolated from soil samples taken from a washwater soakaway at a disused gtn manufacturing plant. the isolates were identified by 16s rrna gene sequence homology as pseudomonas putida, an arthrobacter species, a klebsiella species, and a rhodococcus species. each of the isolates utilized gtn as its sole nitrogen source and removed nitro groups sequentially from gtn to produce glycerol dinitrates a ... | 2001 | 11375172 |
| the sinorhizobium meliloti nutrient-deprivation-induced tyrosine degradation gene hmga is controlled by a novel member of the arsr family of regulatory genes. | the regulation of the nutrient-deprivation-induced sinorhizobium meliloti homogentisate dioxygenase (hmga) gene, involved in tyrosine degradation, was examined. hmga expression was found to be independent of the canonical nitrogen regulation (ntr) system. to identify regulators of hmga, secondary mutagenesis of an s. meliloti strain harboring a hmga-luxab reporter gene fusion (n4) was carried out using transposon tn1721. two independent tn1721 insertions were found to be located in a positive re ... | 2001 | 11375175 |
| biotransformation of d-limonene to (+) trans-carveol by toluene-grown rhodococcus opacus pwd4 cells. | the toluene-degrading strain rhodococcus opacus pwd4 was found to hydroxylate d-limonene exclusively in the 6-position, yielding enantiomerically pure (+) trans-carveol and traces of (+) carvone. this biotransformation was studied using cells cultivated in chemostat culture with toluene as a carbon and energy source. the maximal specific activity of (+) trans-carveol formation was 14.7 u (g of cells [dry weight])(-1), and the final yield was 94 to 97%. toluene was found to be a strong competitiv ... | 2001 | 11375201 |
| is fatal rhodococcus equi pneumonia of foals only an infection acquired by the perinate? | | 2001 | 11380022 |
| application of sartwell's model (lognormal distribution of incubation periods) to age at onset and age at death of foals with rhodococcus equi pneumonia as evidence of perinatal infection. | the distributions of the incubation periods for infectious and neoplastic diseases originating from point-source exposures, and for genetic diseases, follow a lognormal distribution (sartwell's model). conversely, incubation periods in propagated outbreaks and diseases with strong environmental components do not follow a lognormal distribution. in this study sartwell's model was applied to the age at onset and age at death of foals with rhodococcus equi pneumonia. the age at onset of clinical si ... | 2001 | 11380023 |
| chromosome topology and genome size of selected actinomycetes species. | information about the genome organization of actinomycetes species is restricted to a few genera: corynebacterium, mycobacterium, rhodococcus, saccharopolyspora and streptomyces. streptomyces species and saccharopolyspora erythraea were shown to contain a single linear 8 mb chromosome. in contrast, the corynebacterium, mycobacterium and rhodococcus species studied were demonstrated to possess a smaller (3 mb-6.5 mb) single circular chromosome. to investigate whether linear chromosome topology an ... | 2000 | 11386344 |
| genomic and phenomic differentiation of rhodococcus equi and related strains. | 16s rdna sequence and pyrolysis mass spectrometric analyses were carried out on representatives of rhodococcus equi and marker strains of genera that encompass mycolic acid containing actinomycetes. the r. equi strains formed a monophyletic clade within the evolutionary radiation occupied by members of the genera nocardia and rhodococcus. the 16s rdna sequence data also showed r. equi to be an heterogeneous taxon. this heterogeneity was underscored by the pyrolysis mass spectrometric data. these ... | 2000 | 11386356 |
| the diversity of extradiol dioxygenase (edo) genes in cresol degrading rhodococci from a creosote-contaminated site that express a wide range of degradative abilities. | analysis of the bacterial population of soil surface samples from a creosote-contaminated site showed that up to 50% of the culturable micro-organisms detected were able to utilise a mixture of cresols. from fifty different microbial isolates fourteen that could utilise more than one cresol isomer were selected and identified by 16s rrna analysis. eight isolates were rhodococcus strains and six were pseudomonas strains. in general, the rhodococcus strains exhibited a broader growth substrate ran ... | 2000 | 11386357 |
| in situ polychlorophenol bioremediation potential of the indigenous bacterial community of boreal groundwater. | the composition and chlorophenol-degrading potential of groundwater bacterial community in a permanently cold, oxygen-deficient chlorophenol contaminated aquifer at kärkölä, finland was studied with the aim of evaluating in situ bioremediation potential. the groundwater contained from 10(4) to 10(7) microscopically counted cells/ml and up to 10(5) cfu/ml heterotrophic bacteria cultivable at 8 and 20 degrees c. of the 102 pure cultures, of which 86% gram-negative, from the plume area (10,000 micr ... | 2001 | 11394785 |