| microscopic changes of the urinary bladder in patients with primary tumors locally treated with corynebacterium parvum. | the study covered 34 patients with tumors of the urinary bladder treated surgically by transurethral resection and, as an adjuvant therapy, with corynebacterium parvum intravesically administered. microscopic observations were performed on smears stained with blue polychrome-tannin and the histologic study used preparata stained with hematoxylin-eosin and van gieson. after the second series of instillations, many biopsies from the same patient were taken. the investigation revealed the negativit ... | 1989 | 2533966 |
| [mechanism of azelaic acid action in acne]. | the physiopathologic mechanism of acne seems to be dependent on four main factors: a) sebum production and excretion; b) type of keratinization of the follicular channel; c) microbial colonization of the pilosebaceous unit and d) inflammatory reaction of the perifollicular area. azelaic acid is effective in the treatment of acne because it possesses an activity against all of these factors. azelaic acid is a competitive inhibitor of mitochondrial oxidoreductases and of 5 alpha-reductase, inhibit ... | 1989 | 2534550 |
| effect of sodium diethyldithiocarbamate, corynebacterium parvum and mycobacterium cell wall extract on in vitro blastogenic responses of bovine blood lymphocytes. | in vivo inoculation of three-month-old calves with sodium diethyldithiocarbamate (dtc), killed corynebacterium parvum or mycobacterium cell wall extract (mcwe) resulted in an enhancement of in vitro peripheral blood lymphocyte blastogenic responses to mitogens phytohemagglutinin (pha) and concanavalin a (con a) in the first three days after treatment. in a separate experiment, blood lymphocytes isolated from a healthy nontreated calf were incubated in vitro in presence of each of the same immuno ... | 1989 | 2536308 |
| delta-9-tetrahydrocannabinol inhibits macrophage protein expression in response to bacterial immunomodulators. | the objective of this study was to determine the effect of delta-9-tetrahydrocannabinol (delta-9-thc), the major psychoactive component of marihuana, on macrophage protein expression in response to bacterial immunomodulators. peritoneal macrophages of (b6c3)f1 mice receiving propionibacterium acnes exhibited a novel protein profile when compared to resident or vehicle-treated macrophages. in contrast, macrophages from mice treated with p. acnes in concert with delta-9-thc exhibited profiles for ... | 1989 | 2537903 |
| regulation of macrophage functions by l-arginine. | sites of inflammation with prominent macrophage infiltration, such as wounds and certain tumors, are uniquely deficient in free arginine. the effects of arginine availability on macrophage physiology were investigated. when cultured in media containing less than 0.1 mm l-arginine, rat resident peritoneal macrophages exhibited enhanced spreading, tumor cytotoxicity, superoxide production, phagocytosis, and protein synthesis. thus, arginine concentrations similar to those found in sites of inflamm ... | 1989 | 2538541 |
| aetiology of kawasaki disease. | thirteen serum samples from nine children with kawasaki disease and 23 control samples gave negative results on screening for antibodies to hog cholera virus, border disease of sheep, bovine diarrhoea virus, and equine arteritis virus. the sera from two children with kawasaki disease were cytotoxic; a possible link with cytotoxin from propionibacterium acnes is considered. | 1989 | 2539788 |
| ph dependence of the kinetic parameters for the pyrophosphate-dependent phosphofructokinase reaction supports a proton-shuttle mechanism. | the ph dependence of kinetic parameters for the pyrophosphate-dependent phosphofructokinase from propionibacterium freudenreichii suggests that the enzyme catalyzes its reaction via general acid-base catalysis with the use of a proton shuttle. the base is required unprotonated in both reaction directions. in the direction of fructose 6-phosphate phosphorylation the base accepts a proton from the hydroxyl at c-1 of f6p and then donates it to protonate the leaving phosphate. whether this occurs in ... | 1989 | 2548569 |
| bronchoalveolar leukocyte response in experimental silicosis: modulation by a soluble aluminum compound. | the biological properties of quartz have been related to its surface reactivity. we have addressed the role of particle surface reactivity in mediating the biological activity of quartz in mixed dusts, by treating the quartz with aluminum lactate. intratracheal instillation of untreated quartz in rats caused a rapid, sustained alveolitis and bronchoalveolar leukocytes obtained from these animals had enhanced activity in degrading fibronectin, but reduced ability to mount a respiratory burst. qua ... | 1989 | 2552617 |
| susceptibility of anaerobic bacteria to meropenem. | the activity of meropenem was determined against 350 strains of anaerobic bacteria by an agar dilution method. it was compared with those of piperacillin, cefoxitin, imipenem, clindamycin, metronidazole and chloramphenicol. meropenem and imipenem were the most active agents tested. on the basis of these results, meropenem appears to be a promising antimicrobial agent for anaerobic infections and warrants further clinical investigation. | 1989 | 2553656 |
| methylmalonyl-coa mutase from propionibacterium shermanii. evidence for the presence of two masked cysteine residues. | adenosylcobalamin-dependent methylmalonyl-coa mutase from propionibacterium shermanii contains no intramolecular disulphide bridges, but two of the six thiol groups in the heterodimer are only revealed after reduction of the denatured enzyme with dithiothreitol. the available evidence suggests that they are present in disulphide linkages to unknown thiols of low mr. the two specifically masked cysteine residues are cys-535 in the alpha-subunit and cys-517 in the beta-subunit, which occupy exactl ... | 1989 | 2569860 |
| cloning and structural characterization of the genes coding for adenosylcobalamin-dependent methylmalonyl-coa mutase from propionibacterium shermanii. | the structural genes coding for both subunits of adenosylcobalamin-dependent methylmalonyl-coa mutase from the gram-positive bacterium propionibacterium shermanii have been cloned, with the use of synthetic oligonucleotides as primary hybridization probes. the genes are closely linked and are transcribed in the same direction. nucleotide sequence analysis of 4.5 kb of dna encompassing both genes allowed us to infer the complete amino acid sequence of the two subunits: the beta-subunit is the pro ... | 1989 | 2569861 |
| subunit interactions in propionibacterium shermanii methylmalonyl-coa mutase studied by analytical ultracentrifugation. | the effect of increasing ionic strength on adenosylcobalamin-dependent methylmalonyl-coa mutase from propionibacterium shermanii was studied by using analytical ultracentrifugation. both sedimentation-velocity and low-speed sedimentation-equilibration measurements show that the enzyme dissociates progressively into its two dissimilar subunits with increasing ionic strength. equilibrium between the alpha beta-dimer and the separated subunits is rapidly established under these conditions. dissocia ... | 1989 | 2569862 |
| changes in number and density of large granular lymphocytes upon in vivo augmentation of mouse natural killer activity. | nk activity of mice as well as humans and rats has been clearly associated with large granular lymphocytes (lgl). to better understand the effects of interferon (ifn) and ifn inducers on natural killer (nk) cells, we have compared the lgl in the spleens of normal and boosted mice. cells were fractionated by centrifugation on discontinuous percoll density gradients, and each fraction was tested for nk activity against yac-1 targets and for the presence of lgl. in vivo treatment with c. parvum (0. ... | 1985 | 2579157 |
| immunologic aspects of gynecologic cancer. | | 1985 | 2579358 |
| recent developments in immunomodulatory therapy. | | 1985 | 2579994 |
| anaerobic bacterial bacteremia: 12-year experience in two military hospitals. | clinical and microbiologic data of 296 patients with anaerobic bacteremia were reviewed. anaerobes were isolated with aerobic or facultative bacteremia in 23 instances. the bacteroides fragilis group accounted for 148 (70%) of 212 isolates of bacteroides species. b. fragilis accounted for 78% and b. thetaiotaomicron for 14%. among other species, there were 20 (6%) fusobacterium organisms, 63 (18%) clostridium isolates, and 53 (15%) anaerobic cocci. seventy-five patients died: 40 had b. fragilis ... | 1989 | 2584754 |
| [studies of the effects of bcg and corynebacterium parvum vaccines in experimental infection of mice with influenza virus. foot pad test, splenic indicator and histologic changes in the thymus gland, spleen and lymph nodes]. | evaluation of the influence of bcg and coparvax on reticulo-endothelial system in mouse was performed. mice were stimulated i.p. with bcg vaccine and coparvax vaccine. spleen index and histological changes in thymus, spleen and lymph nodes were evaluated after 14 days in mice vaccinated with bcg and after 7 days in mice vaccinated with coparvax. foot pad test was also performed by giving vaccine into three feet. tuberculin was injected into mouse foot pad on the day 7th and 14th and a lysate of ... | 1989 | 2586194 |
| effect of intracerebrally injected corynebacterium parvum on the development and growth of metastatic brain tumor in mice. | using kht tumor in a mouse metastatic tumor model, we examined the effect of intracerebral and/or intraperitoneal injections of corynebacterium parvum on the growth of metastatic brain tumor and the development of an inflammatory response in the central nervous system (cns). c. parvum given intraperitoneally had no effect on the development and growth of cns tumor, but did prolong the survival of mice by inhibiting the growth of systemic metastatic tumor, which was the cause of death in our tumo ... | 1989 | 2586725 |
| comparison of the nasal bacterial floras in two groups of healthy subjects and in patients with acute maxillary sinusitis. | the nasal bacterial flora was studied in 183 healthy men entering military service (entry group), 103 healthy recruits in service (service group), and 185 recruits with acute maxillary sinusitis. the 267 nasal and ipsilateral sinus aspirate findings in the same patients with acute maxillary sinusitis were compared pairwise. in the entry group presumed sinus pathogens were only rarely isolated from the nasal cavities: haemophilus influenzae in 4%, streptococcus pneumoniae in 1%, branhamella catar ... | 1989 | 2592539 |
| studies on antibacterial agents. i. synthesis of substituted 6,7-dihydro-1-oxo-1h,5h-benzo[i,j]quinolizine-2-carboxylic acids. | a series of substituted 6,7-dihydro-1-oxo-1h,5h-benzo[i,j]quinolizine-2-carboxylic acids was synthesized and tested for antibacterial activities. among them, 9-fluoro-6,7-dihydro-5-methyl-8-(4-methyl-1-piperazinyl)-1-oxo-1h,5h- benzo[i,j]quinolizine-2-carboxylic acid (opc-7241) exhibited potent antibacterial activity against gram-positive and -negative bacteria, including staphylococcus aureus and pseudomonas aeruginosa, and 9-fluoro-6,7-dihydro-8-(4-hydroxy-1-piperidyl)-5-methyl-1-oxo-1h, 5h-be ... | 1989 | 2598310 |
| studies on route of immunization with a mixture of killed parasites and adjuvants against babesia rodhaini infection in mice. | a series of experiments were undertaken to determine the most effective route of immunization with a mixture of killed babesia rodhaini antigen (s antigen) and formalin-fixed corynebacterium parvum (propionibacterium acnes) bacterin (cpb) against challenge infection with b. rodhaini 3 weeks later. the mice pretreated with s antigen and cpb mixture intraperitoneally, but not intramuscularly, were significantly resistant to intraperitoneal (ip) or intravenous (iv) challenge with 10(6) organisms. t ... | 1989 | 2601229 |
| effect of corynebacterium parvum bacterin on artificial immunity against babesia rodhaini infection in mice. | | 1989 | 2601237 |
| effects of synthetic retinoids on the growth of bacteria and their susceptibility to antibiotics. | in patients treated with oral retinoids the recovery of propionibacterium acnes and other anaerobic bacteria in the skin is markedly reduced, whereas an increased colonization of the skin and a significant rise in the incidence of cutaneous staphylococcal infections are observed. since very little is known about the effects of retinoids on bacteria, in the present study we investigated the influence of 4 retinoids (isotretinoin, etretinate, arotinoid ethyl ester, arotinoid sulfone) in 15 differe ... | 1989 | 2614503 |
| propionibacterium acnes endophthalmitis: an unusual manner of presentation. | an unusual clinical presentation of propionibacterium acnes endophthalmitis as a solitary nodule on the corneal endothelial surface at the superior limbus in association with granulomatous uveitis is reported. the patient had previous extracapsular cataract surgery with intraocular lens implantation and two subsequent yag laser capsulotomies. the organism was grown from a microbiopsy of the nodule after a negative anterior chamber tap. the patient was treated with systemic antibiotics, his condi ... | 1989 | 2614715 |
| dissimilatory nitrate reduction by propionibacterium acnes. | propionibacterium acnes p13 was isolated from human feces. the bacterium produced a particulate nitrate reductase and a soluble nitrite reductase when grown with nitrate or nitrite. reduced viologen dyes were the preferred electron donors for both enzymes. nitrous oxide reductase was never detected. specific growth rates were increased by nitrate during growth in batch culture. culture ph strongly influenced the products of dissimilatory nitrate reduction. nitrate was principally converted to ni ... | 1989 | 2624464 |
| studies on the biosynthesis of corrinoids and porphyrinoids. i. the labeling of oxygen of vitamin b12. | recently the amide-oxygen has been suggested to participate in the formation of the corrin ring of vitamin b12. to confirm this hypothesis, 17o-labeled aminolevulinic acid (ala) was prepared and administered to propionibacterium shermanii. the isolated vitamin b12 showed only broad 17o signals in the oxygen-17 nuclear magnetic resonance (17o-nmr) spectrum. however, distinct isotope-shifted peaks were observed in the 13c-nmr spectrum of vitamin b12 isolated after incorporation of [1-13c:1,4-18o2] ... | 1989 | 2630086 |
| [bacteriogenic premature rupture of the fetal membranes]. | | 1989 | 2636019 |
| [cariogenicity of propionibacterium acnes, streptococcus intermedius and streptococcus mutans in germ-free rats]. | a comparative study has been made on the cariogenic potentials of propionibacterium acnes atcc 11828, streptococcus intermedius oe-1 and streptococcus mutans oz-1. the strains of p. acnes and s. intermedius have chondroitinase activity. as metabolic end-products, the p. acnes strain produces mainly a weak acid, propionic acid, whereas strong lactic acid is the main product of the strains of s. intermedius and s. mutans. five germ-free rats were used for infection with each bacterial strain. the ... | 1989 | 2637631 |
| antimicrobial action of natural substances on oral bacteria. | the antimicrobial action of natural substances was investigated in vitro against oral bacteria including streptococcus sp., actinomyces sp., actinobacillus sp., bacteroides sp., capnocytophaga sp., eikenella sp., fusobacterium sp. and propionibacterium sp. among the natural substances tested, hinokitiol was the most inhibitory to oral bacteria. cinnamon bark oil, papua-mace extracts, and clove bud oil in spice extracts were also inhibitory against many oral bacteria. egg white lysozyme exhibited ... | 1989 | 2637783 |
| protection of mice against vaccinia and herpes simplex virus infection by propionibacterium acnes. | heat-killed cell suspension of several propionibacterium acnes strains were prepared and studied for their protective activity in viral infection of mice and immunomodulating properties. majority of the strains caused marked spleen enlargement in the treated mice. these changes persisted for several weeks. only some of the tested strains enhanced significantly primary humoral immune response against sheep red blood cells. there was no increase, however, in neutralizing or hemagglutination-inhibi ... | 1989 | 2639640 |
| [preliminary studies of the effect of steroid hormones on skin bacteria in vitro]. | it results from studies on skin hormones that some of steroid hormones reach high concentrations on skin surface mainly during a course of acne vulgaris. our studies indicate that the activity of hormones on skin bacteria can be multiform. nineteen hormones of analytical grade of purity were tested. they were derived from different firms of synthesized in the department of endocrinology of the pharmacology institute of polish academy of sciences in cracow. bacterial strains tested in this study ... | 1989 | 2640684 |
| propionibacterium acnes immunotherapy in chronic recurrent canine pyoderma. an adjunct to antibiotic therapy. | in a randomized, double-blind, placebo-controlled study, dogs with chronic recurrent pyoderma were treated with antibiotics plus intravenous injections of either propionibacterium acnes or placebo. responses (an increase, decrease, or clearing of lesions) were measured and evaluated statistically. eighty percent (12 of 15) of the dogs treated with antibiotics and p acnes compared with 38% (five of 13) of the dogs treated with antibiotics and placebo responded with significant improvement or comp ... | 1989 | 2647969 |
| regulation of insulin and interleukin-1 release after propionibacterium acnes-induced macrophage activation in mice. | the administration of a potent activator of macrophages (m phi), propionibacterium acnes, in nondiabetic mice was associated with the release of significant amounts of interleukin-1 (il-1) in the peritoneal cavity and plasma within 4 hours after treatment. shortly before il-1 peaks were observed, the levels of pancreatic insulin, [3h]leucine-proinsulin, and insulin/total protein ratio were elevated, and followed by a transient but marked hyperinsulinemia at 4 hours after treatment. a single dose ... | 1989 | 2648066 |
| soluble malarial antigens are toxic and induce the production of tumour necrosis factor in vivo. | heat-stable soluble products of rodent malarial parasites induce activated peritoneal macrophages to secrete tumour necrosis factor (tnf) in vitro. since heat-stable parasite antigens are known to be present in the circulation of patients with malaria and it has been suggested that much of the pathology of malaria is due to tnf, we investigated the ability of such antigens to induce the production of tnf in vivo and to be toxic to mice. injection of antigens obtained from plasmodium yoelii or fr ... | 1989 | 2654012 |
| propionibacterium acnes in sarcoidosis. | | 1989 | 2657925 |
| intravascular coagulation in the development of massive hepatic necrosis induced by corynebacterium parvum and endotoxin in rats. | when escherichia coli endotoxin was intravenously injected into rats given killed corynebacterium parvum 6 days previously, fibrin deposition and endothelial cell injury occurred in hepatic sinusoids at 1.5 h and were intensified thereafter. serum alanine aminotransferase values were increased along with prothrombin time and decreased plasma levels of antithrombin iii and coagulation factor viii:c at 5 h. antithrombin iii concentrate (plus heparin) or superoxide dismutase infused concurrently wi ... | 1989 | 2660248 |
| characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic balb/c hosts. | a mouse mammary tumor cell line, designated jc, has been established from a spontaneously developed primary adenocarcinoma of an aged virgin female balb/c mouse. isoenzyme analyses including glucose-6-phosphate dehydrogenase, lactate dehydrogenase, and peptidase proved that this cell line is of murine origin and devoid of contamination from other species. karyotyping revealed that the number of chromosome ranged from 26 to 100, with a modal number of 40. electron microscopic examination detected ... | 1989 | 2666384 |
| propionibacterium acnes causes postoperative brain abscesses unassociated with foreign bodies: case reports. | propionibacterium acnes was isolated in pure culture from brain abscesses that occurred in two patients after intracranial surgery. although such infections are usually associated with cerebrospinal fluid shunts and other foreign bodies, these cases clearly demonstrate the pathogenicity of this anaerobic diphtheroid in the absence of such. progression of p. acnes infection in the central nervous system can be insidious. to treat such infections adequately, therapy cannot rely upon some standard ... | 1989 | 2666880 |
| [bacterial skin flora, host defense and skin infections]. | resident skin flora consists of coagulase-negative staphylococci, micrococci, aerobic and anaerobic coryneform organisms (i.e. propionibacterium acnes) as well as gram-negative rods (i.e. acinetobacter). their growth is favoured by increased temperature and humidity and modified by body location, age, sex and chronic diseases (i.e. diabetes mellitus). occupation, hospitalization, soaps and disinfectants as well as medications exert promoting and inhibiting influences, too. in addition, environme ... | 1989 | 2693140 |
| suppression of resistance to salmonella typhimurium in young chickens inoculated with corynebacterium parvum. | the effect of corynebacterium parvum on resistance to salmonella typhimurium infection was evaluated in young chickens. one-day-old chickens were inoculated subcutaneously (sc) or intraperitoneally (ip) with 1.4 mg killed c. parvum and challenged by ip injection with 5.0 x 10(7) s. typhimurium 4 days later. spleen and bursa of fabricius weights were not altered in the c. parvum-inoculated chickens. a transient increase in thymus weight occurred 3 days after inoculation with c. parvum. phytohemag ... | 1989 | 2695051 |
| delayed cerebrospinal-fluid shunt infection in children. | twelve patients with shunt infection occurring more than 6 months following shunt implantation or revision were identified and their charts reviewed. these cases were accumulated over a 9-year period, and delays from shunt surgery to detection were as long as 11 years. for 5 patients, no antecedent infection or surgery could be identified as a presumptive cause of the shunt infection. propionibacterium species and staphylococcus epidermidis species were the most common organisms identified. the ... | 1989 | 2702349 |
| role of respiratory-burst products from polymorphonuclear leukocytes in the antitumor activity of propionibacterium acnes vaccine. | tumor cells injected into balb/c mice together with heat-killed 48-h p. acnes cells were rendered nontumorigenic as early as 12 h after injection, as determined by the inability of the tumor cells to give rise to tumors when transferred to a new host. determination of tumor cell antigen levels by elisa indicated that the tumour antigens had virtually disappeared by 24 h after injection of tumor cells and p. acnes. in contrast, in control animals injected with tumor cells only, there was an initi ... | 1989 | 2706641 |
| [sepsis due to propionibacterium acnes?]. | | 1989 | 2707126 |
| effects of bile acids on liver cell injury by cultured supernatant of activated liver adherents cells. | when heat-killed propionibacterium acnes (p. acnes) is intravenously injected into mice followed by an intravenous injection of a small amount of lipopolysaccharide (lps) 7 days later, most of the mice die of massive hepatic cell necrosis within 24 hours of lps injection. in addition, when the liver adherent cells including kupffer cells are separated from the mice 7 days after p. acnes injection and incubated in vitro with lps, remarkable activity of the cytotoxic factor is found in the culture ... | 1989 | 2707549 |
| activation of murine peritoneal macrophages by saikosaponin a, saikosaponin d and saikogenin d. | macrophage activation by saikosaponins and saikogenins was investigated and compared with that by other saponins and macrophage stimulants. saikosaponins a and d induced a marked cell accumulation in the peritoneal cavity when administered intraperitoneally. among saikosaponins and saikogenins tested, saikosaponin d significantly activated peritoneal macrophages in terms of enhancement of phagocytic activity, increased level of cellular lysosomal enzyme (acid phosphatase), induction of cytostati ... | 1989 | 2707937 |
| automated analysis of 2,3-diamino-2,3-dideoxy-d-glucuronic acid by cation exchange chromatography with fluorometric postcolumn derivatization. | 2,3-diamino-2,3-dideoxy-d-glucuronic acid (diaminoglucuronic acid) occurs as its di-n-acetyl derivative as a unique constituent of some bacterial cell walls. a sensitive chromatographic method for its determination is described. diaminoglucuronic acid was well separated from glucosamine and galactosamine in about 80 min on a dionex dc-6a cation exchange column (0.9 x 18 cm, 50 degrees c) with a sodium citrate buffer (ph 5.28) containing boric acid (0.2 m). the amino sugars in the eluate were mon ... | 1989 | 2712291 |
| hyperthermia induced polyphosphate changes in propionibacterium acnes as studied by 31p nmr. | the polyphosphate component in 31p nmr spectra of the gram-positive propionibacterium acnes increased after hyperthermia treatment. the cells were exposed to temperatures in the interval from 15 degrees c to 45 degrees c. the amount of polyphosphate increased with increasing temperature. there were no temperature induced changes in the other phosphorous components seen in the spectra with exception of a decrease in atp for higher temperatures. the increase in polyphosphates was less than that ob ... | 1989 | 2712997 |
| role of novel adjuvants in specific active immunotherapy. | | 1989 | 2717639 |
| role of lipids in augmenting the antibacterial activity of benzoyl peroxide against propionibacterium acnes. | antimicrobial activity and physicochemical properties of benzoyl peroxide (bp) were investigated to determine the mechanism of action for the compound as an antiacne agent. the mics and mbcs against nine strains of propionibacterium acnes ranged between 100 and 800 micrograms/ml in a nutrient broth system, with a median fourfold increase in activity demonstrated when lipid was added. the partition coefficient of bp in a 50:50 artificial skin lipid and water system was greater than 2,500, with th ... | 1989 | 2729927 |
| augmentation of mouse liver-associated natural killer activity by biologic response modifiers occurs largely via rapid recruitment of large granular lymphocytes from the bone marrow. | a variety of biologic response modifiers (brm) can potently augment nk activity in nonlymphoid organs. by using the liver as a model organ, we have shown that this augmentation of organ-associated nk activity is coincident with a 10- to 15-fold increase in the number of large granular lymphocytes (lgl) which can be isolated. the present study was designed to investigate the mechanism by which brm induce this increase in liver-associated lgl and the coincident increase in hepatic nk activity. ini ... | 1989 | 2732472 |
| lymphocytes generated by in vivo priming and in vitro sensitization demonstrate therapeutic efficacy against a murine tumor that lacks apparent immunogenicity. | the adoptive transfer of sensitized lymphocytes is an effective means to mediate the regression of established tumors. however, successful therapy can only be demonstrated in animal models where tumors are intrinsically immunogenic, capable of eliciting systemic immunity. to explore the potential of this therapeutic approach to tumors of less immunogenicity, we have selected and used a murine tumor, mca 102, for the current study because all attempts to immunize syngeneic mice failed. we report ... | 1989 | 2738408 |
| focal infections of the central nervous system due to propionibacterium acnes. | propionibacterium acnes as the single cause of intracranial infections has rarely been described. we present four patients in whom the infection arose as a result of a breach of skin tegument, and postulate that p. acnes is an under-recognised pathogen. | 1989 | 2745987 |
| chemiluminescence in a macrophage cell line modulated by biological response modifiers. | we have studied a murine macrophage cell line, j774, and found these cells capable of a zymosan-triggered chemiluminescent oxidative burst. such activity was enhanced by preincubation with corynebacterium parvum (cp), bacillus calmette-guerin, and lipopolysaccharide (lps). under similar conditions, cp and lps were shown to enhance j774-mediated tumor cell lysis. we have also demonstrated that murine interferon alpha + beta rendered j774 cells more sensitive to the actions of cp and lps. these re ... | 1989 | 2746137 |
| [antibody activity to propionibacterium acnes in bronchoalveolar lavage fluid in sarcoidosis]. | while increased levels of circulating antibody to various microorganisms have been reported in sarcoidosis patients, the pathogenesis of the disease is still unknown. in this report, the levels of antibody activities against propionibacterium acnes (p. acnes) were measured in bronchoalveolar lavage fluid (balf) in patients with sarcoidosis, using an enzyme-linked immunosorbent assay method. each immunoglobulin class of antibody activity to p. acnes was corrected by albumin concentrations in balf ... | 1989 | 2747059 |
| serotonin and tryptamine metabolism in the acute hepatic failure model: changes in tryptophan and its metabolites in the liver, brain and kidney. | when heat-killed propionibacterium acnes, a gram-positive anaerobe, is intravenously injected into mice followed by an intravenous injection of gram-negative lipopolysaccharide (lps) 7 days later, most of the mice die of massive hepatic cell necrosis within 24 hours of lps injection. using this experimental model, acute hepatic failure was induced in mice, and the tryptophan metabolism in the liver, brain and kidney was studied. as a result, the tryptophan level was remarkably high in all three ... | 1989 | 2748146 |
| non-specific resistance to babesia divergens in the mongolian gerbil (meriones unguiculatus). | inoculation of mature gerbils with bcg gave protection to subsequent infection with b. divergens when inoculated by the intracardiac and intraperitoneal routes, the latter showing a dose dependent relationship. bcg vaccination was most effective in immature gerbils (less than 4 weeks old), which are innately resistant to b. divergens. vaccination of gerbils with killed propionesbacterium acne and zymosan a failed to elicit a protective response, which contrasts conspicuously with rodent babesia ... | 1989 | 2759766 |
| colony counts and characterization of bacteria adherent to the rumen wall and desquamated epithelial cells in conventional young lambs. | characterization and enumeration of the adherent epimural community of the rumen wall of young, conventionally reared lambs were carried out from 2 to 21 days after birth. three hundred strains were isolated by anaerobic procedures from three sites: dorsal, ventral, and caudal sacs, and from the sloughed epithelial cells. the population of epimural bacteria was very dense from the first days of the lamb's life. this population increased slightly with age. during the first week the counts were si ... | 1989 | 2766120 |
| recent studies on the microbial ecology of the upper gastrointestinal tract. | microecological problems have escalated recently in clinical medicine. we studied the microecology of the upper small bowel. duodenal flora was examined, displaying microbial growth in 90.8% of the 400 patients examined. the microflora was classified according to germ quality into definite types of colonization. frequency of microbial colonization is normally 36.7% and 63.3% by overgrowth, indicating differing dysbioses. simulation of microbial overgrowth in the duodenum is possible with a conti ... | 1989 | 2767771 |
| [changes in production of platelet activating factor by adherent hepatocytes of mice with experimental liver failure]. | it is thought that the platelet activating factor (paf) enhances the immune response and inflammatory reaction. we studied the production of paf from liver adherent cells. as a result, liver adherent cells produced paf, when they were stimulated with calcium ionophore. in addition, when mice were treated with heat-killed propionibacterium acnes and the mononuclear cells were infiltrated into the liver, a significantly larger amount of paf was produced compared to normal mice. | 1989 | 2770018 |
| diphtheroids as ocular pathogens. | unlike corynebacterium diphtheriae and propionibacterium acnes, the pleomorphic gram-positive rods known as diphtheroids are generally regarded as nonpathogenic contaminants of the human external eye. we reviewed five years of microbiology records at wills eye hospital and studied a series of eight cases of apparently infectious keratitis associated with heavy growth of diphtheroids on cultures of ulcer scrapings. all of these cases included indolent ulcers that occurred almost exclusively in el ... | 1989 | 2774033 |
| coaggregation of fusobacterium nucleatum, selenomonas flueggei, selenomonas infelix, selenomonas noxia, and selenomonas sputigena with strains from 11 genera of oral bacteria. | twenty-eight strains of fusobacterium nucleatum and 41 selenomonas strains, including s. sputigena (24 strains), s. flueggei (10 strains), s. infelix (5 strains), and s. noxia (2 strains), were tested for their ability to coaggregate with each other and with 49 other strains of oral bacteria representing actinobacillus, actinomyces, bacteroides, capnocytophaga, gemella, peptostreptococcus, porphyromonas, propionibacterium, rothia, streptococcus, and veillonella species. selenomonads coaggregated ... | 1989 | 2777378 |
| propionibacterium-associated endophthalmitis after extracapsular cataract extraction. review of reported cases. | sixteen cases of previously reported culture-proven propionibacterium-associated endophthalmitis after extracapsular cataract extraction (ecce) are reviewed. the inflammation was observed 2 to 10 months after surgery and occurred after laser posterior capsulotomy in four cases. clinically, it appeared as a chronic iridocyclitis characterized by granulomatous-appearing keratic precipitates (5 cases), hypopyon (10 cases), and a white plaque on the posterior capsule or intraocular lens (iol) implan ... | 1989 | 2783995 |
| hematologic interactions of endotoxin, tumor necrosis factor alpha (tnf alpha), interleukin 1, and adrenal hormones and the hematologic effects of tnf alpha in corynebacterium parvum-primed rats. | endotoxin reduces the release among other cytokines of tumor necrosis factor (tnf) and interleukin 1 (il-1) and causes peripheral lymphopenia and a dose-response-dependent initial neutropenia followed by a monophasic neutrophilia. tnf alone induces lymphopenia and an initial neutropenia followed by a biphasic neutrophilia. il-1 alone induces lymphopenia and a monophasic neutrophilia. tnf-plus-il-1 caused a greater lymphopenia than either monokine alone, suggesting that both monokines contribute ... | 1989 | 2786048 |
| analysis of the proinflammatory property of epidermal cyst contents: chemotactic c5a anaphylatoxin generation. | we investigated in vitro the contents of epidermal cysts for complement activation and found that they activated complement mainly through the alternative pathway. chemotactic c5a anaphylatoxin produced by the cyst contents after contact with serum most likely plays a significant role in the initiation and aggravation of inflammation in ruptured epidermal cysts. our additional study disclosed that components of three representative follicular resident microorganisms (pityrosporum ovale, propioni ... | 1989 | 2786384 |
| [interleukin-2 production and receptor expression of alveolar lymphocytes stimulated by propionibacterium acnes in sarcoidosis]. | we previously reported that alveolar lymphocytes in patients with active sarcoidosis are sensitized to propionibacterium acnes (p. acnes) which may play a significant role in the induction of alveolitis in these patients. however, the mechanism of lymphocyte activation is not fully understood. in this study, we further investigated the production of interleukin-2 (il-2), and the responsiveness to il-2 of alveolar lymphocytes obtained from sarcoidosis patients and stimulated by p. acnes in vitro. ... | 1989 | 2787441 |
| [infections by anaerobes in children: frequency of isolation and antimicrobial resistance]. | we studied the frequency of isolation and antimicrobial susceptibility of strict anaerobic bacteria isolated in a paediatric hospital. a total of 1,753 samples from purulent material and hemocultures were processed. one hundred and thirty strains were isolated from 95 children: 44 from 48 cases of peritonitis (91.7%), 2 from 8 brain abscesses (25%), 23 from 124 soft tissue abscesses (18.5%), 4 from 64 empyemas or lung abscesses (6.3%) and 22 from 1,509 hemocultures (1.5%). mixed infection (anaer ... | 1989 | 2788434 |
| management options for propionibacterium acnes endophthalmitis. | the authors reviewed the management of nine culture-proven cases of propionibacterium acnes endophthalmitis which presented at an average of 4 months after extracapsular cataract extraction (ecce) and posterior chamber intraocular lens (pc iol) implantation. the initial signs included a white intracapsular plaque (9/9), vitritis (9/9), granulomatous uveitis (4/9), nongranulomatous uveitis (5/9), hypopyon (4/9), beaded fibrin strands in the anterior chamber (3/9), and diffuse intraretinal hemorrh ... | 1989 | 2788852 |
| [comparison of the effectiveness and adverse effects of a corynebacterium parvum vaccine made in czechoslovakia with coparvax, a british preparation made by wellcome, in the treatment of malignant pleural effusions]. | malignant pleural effusion complicates various malignant diseases, most frequently lung and breast cancer. a group of 20 patients with this complication were treated with the czechoslovak vaccine corynebacterium parvum and the english preparation coparvax, and their effects and side-effects were compared. within 4 weeks following the application, 7 patients died. in 9 surviving patients, the exudate ceased to be replenished and in 4 persons, after intrapleural application of the preparation, it ... | 1989 | 2790915 |
| effects of tetracyclines on the production of extracellular proteins by members of the propionibacteriaceae. | the effect of tetracyclines on the synthesis of proteins in propionibacterium avidum and p. acnes was examined. synthesis of an extracellular lipase by p. avidum was slightly more sensitive to inhibition by tetracycline than total (cellular and extracellular) protein synthesis. the effect of tetracycline and other analogues on the synthesis of secreted proteins was also examined in p. avidum and p. acnes by protein radiolabelling experiments. in all cases the synthesis of secreted proteins was o ... | 1989 | 2792736 |
| toxicity of il-2 and corynebacterium parvum following direct intracranial injection. | the toxicity of il-2 and/or c. parvum when injected directly into the mouse brain was examined by survival and histopathology using different numbers of injections, different doses of il-2, different solvents for the il-2, and different doses and routes of administration of c. parvum. two injections was found significantly to increase mortality (19%) over a single injection (4%). mortality from two injections of 30,000 u (23%) or 60,000 u (20%) was higher than from two injections of 15,000 u (12 ... | 1989 | 2795120 |
| intralenticular abscess. | intralenticular abscess is an uncommon complication of surgery or trauma. a case of intralenticular abscess following penetrating trauma is presented. in this case, the offending organism was identified as propionibacterium acnes. this organism is considered to be a cause of slowly progressing endophthalmitis and has been implicated as a cause of the so-called toxic lens syndrome. | 1989 | 2803777 |
| propionibacterium acnes isolated from explanted intraocular lens in pseudophakic bullous keratopathy. | propionibacterium acnes is an anaerobic bacterium that has been reported to produce ocular infection or inflammation following surgery or penetrating trauma. it has been well documented as an etiologic agent associated with infections of prosthetic implants, including intraocular lenses (iols). p. acnes is part of the natural flora of the external eye and has biological properties that may promote, heighten, or prolong an inflammatory response. it not only resists in vitro degradation by reticul ... | 1989 | 2805713 |
| an experimentally-induced acute hepatic failure model in various strains of mice. | when balb/cajc1 mice are intravenously injected with heat-killed propionibacterium acnes (p. acnes) followed by an intravenous injection of lipopolysaccharide (lps) 7 days later, massive necrosis is induced in the liver tissue and most of the mice die within 24 hours of lps injection. using this experimental model, acute hepatic failure was induced in various strains of mice and the difference in the response was studied. as a result, as in balb/cajc1 mice, acute hepatic failure was also induced ... | 1989 | 2806831 |
| [the response of alveolar lymphocytes induced by propionibacterium acnes in pulmonary sarcoidosis: correlation with clinical studies, pulmonary function studies and bronchoalveolar lavage]. | current concepts of the pathogenesis of sarcoidosis suggest that the alveolitis in this disorder is related to increased numbers of helper t-cells within the lungs. however, the mechanism of this accumulation of lymphocytes is not known. we have reported that proliferation of alveolar lymphocytes induced by p. acnes is increased in patients with active sarcoidosis. to determine whether the response of alveolar lymphocytes would be useful in staging the activity of pulmonary sarcoidosis, we studi ... | 1989 | 2810973 |
| [spondylitis of the cervical spine caused by non-sporogenic anaerobes]. | | 1989 | 2811118 |
| infectious arthritis caused by propionibacterium acnes: a report of two cases. | propionibacterium acnes, a micro-organism with low pathogenicity, rarely causes arthritis in a joint without a prosthesis. this report deals with two cases of p acnes infectious arthritis probably caused by direct penetration of the micro-organism into the joint space during arthrocentesis, despite the use of strict disinfection procedures. p acnes isolated from synovial fluid cannot be dismissed as a bothersome contaminant without due consideration of the clinical status of the individual patie ... | 1989 | 2818021 |
| two-dimensional gel electrophoresis of ribosomal proteins from propionibacterium acnes and granulosum. | ribosomal proteins (r-proteins) from a type i strain (atcc 11827) and a type ii strain (atcc 11828) of propionibacterium acnes (p. acnes) and from a propionibacterium granulosum (p. granulosum) strain (atcc 11829-2) were analyzed using two-dimensional polyacrylamide gel electrophoresis (two-dimensional page), for the purpose of genetical analysis of the p. acnes type i and type ii strains and the p. granulosum strain. among the fifty r-proteins in all, almost all of the proteins were electrophor ... | 1989 | 2818796 |
| inactivation of pyrophosphate-dependent phosphofructokinase from propionibacterium freudenreichii by pyridoxal 5'-phosphate. determination of the ph dependence of enzyme-reactant dissociation constants from protection against inactivation. | the pyrophosphate-dependent phosphofructokinase from propionibacterium freudenreichii is rapidly inactivated by low concentrations of pyridoxal 5'-phosphate (plp). the inactivation is first order with respect to plp and the rate increases linearly with plp concentrations suggesting that over the concentration range used no significant e-plp complex accumulates during inactivation. the rate of inactivation decreases at high and low ph and this is discussed in terms of the mechanism of schiff base ... | 1988 | 2833495 |
| bone marrow derived macrophages have polyamine and ectoenzyme phenotypes distinct from resident macrophages. | several prototype macrophage (mo) populations were compared for differences in ectoenzyme phenotype and polyamine content. resident peritoneal mo and corynebacterium parvum (cp)-activated peritoneal mo expressed unique ectoenzyme phenotypes, while bone marrow derived mo (bmdmo), obtained from stem cells after 7 days in culture with colony stimulating factor, and thioglycollate (tg)-elicited peritoneal mo exhibited a similar ectoenzyme phenotype. all of the mo populations, however, differed in po ... | 1988 | 2835568 |
| the atp/amp binding site of pyruvate,phosphate dikinase: selective modification with fluorescein isothiocyanate. | pyruvate,phosphate dikinase from propionibacterium shermanii is strongly inhibited by fluorescein 5'-isothiocyanate (fitc). the time course of inactivation is biphasic, but the dependence of the pseudo-first-order rate constants on the inhibitor concentration indicates the formation of a reversible complex with the enzyme prior to covalent modification. the substrate/product nucleotide pairs mgatp and mgamp protected against inactivation, while in the absence of mg2+, both the nucleotides were i ... | 1988 | 2839231 |
| isotope exchange as a probe of the kinetic mechanism of pyrophosphate-dependent phosphofructokinase. | data obtained from isotope exchange at equilibrium, exchange of inorganic phosphate against forward reaction flux, and positional isotope exchange of 18o from the bridge position of pyrophosphate to a nonbridge position all indicate that the pyrophosphate-dependent phosphofructokinase from propionibacterium freudenreichii has a rapid equilibrium random kinetic mechanism. the maximum rates of isotope exchange at equilibrium for the [14c]fructose 1,6-bisphosphate in equilibrium fructose 6-phosphat ... | 1988 | 2839232 |
| in vitro activities of daptomycin (ly146032) and paldimycin (u-70,138f) against anaerobic gram-positive bacteria. | the in vitro activities of daptomycin (ly146032), paldimycin (u-70,138f), vancomycin, and penicillin g against 344 clinical isolates of anaerobic gram-positive bacteria were determined by an agar dilution method in calcium-supplemented (50 micrograms/ml) wilkins-chalgren medium, using an inoculum of 10(5) cfu. daptomycin demonstrated excellent activity against a broad range of anaerobic gram-positive cocci and bacilli, including peptostreptococcus, eubacterium, bifidobacterium, actinomyces, prop ... | 1988 | 2840019 |
| effect of macrophage activation on resistance of mouse peritoneal macrophages to infection with herpes simplex virus types 1 and 2. | to define the effect of heterogeneity of murine peritoneal macrophages (m phi) on intrinsic resistance to herpes simplex virus (hsv) infection, several m phi populations were characterized for their response to infection with hsv type 1 (hsv-1) and hsv-2. steady-state resident m phi (res m phi) were compared in parallel with m phi activated with corynebacterium parvum (now designated propionibacterium acnes) (cp m phi) and thioglycollate-elicited inflammatory m phi (tg m phi). res m phi were com ... | 1988 | 2841412 |
| effect of endotoxin on release of reactive oxygen intermediates by rat hepatic macrophages. | reactive oxygen intermediates released by activated hepatic macrophages have been implicated in the pathogenesis of a rat model of liver injury induced by sequential administration of corynebacterium parvum and endotoxin. in this model, c. parvum causes extensive infiltration of the liver with activated macrophages, but severe liver injury occurs only after subsequent exposure to endotoxin. we have therefore investigated the effects of endotoxin on the release of reactive oxygen intermediates by ... | 1988 | 2846404 |
| influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines. | sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ... | 1988 | 2847397 |
| bacterial products as immunomodulating agents. | | 1985 | 2858453 |
| a comparison of the effect of povidone-iodine and 60% n-propanol on the resident flora using a new test method. | the efficacy and safety of povidone-iodine (pvp-i) and 60% v/v% n-propanol on the forehead of patient's were examined using a newly developed method. significantly higher reduction factors (rfs) of the total resident flora were obtained using pvp-i immediately after and 3-6 h after application (1.4, 1.4, 1.4) than with isopropanol (0.6, 0.6, 0.8) using the detergent scrub method. no significant difference was found using the cyanoacrylate method. propionibacter spp. showed similar results. with ... | 1985 | 2860179 |
| adverse effect of intrapleural corynebacterium parvum as adjuvant therapy in resected stage i and ii non-small cell carcinoma of the lung. ludwig lung cancer study group. | intrapleural corynebacterium parvum 7 mg or placebo was administered postoperatively as adjuvant therapy to patients with resected stage i and ii non-small cell lung cancer in a prospective, randomized, multicenter trial. a total of 475 patients were entered into this study between july, 1977, and february, 1979. of this group, 405 can be evaluated, with an average follow-up time of 4.6 years. more side effects, especially fever and chest pain, were observed in patients receiving corynebacterium ... | 1985 | 2860271 |
| the involvement of dendritic cells in the handling of the immune stimulant c. parvum. a morphological investigation using immunoperoxidase techniques. | in earlier experiments we showed that locally administered corynebacterium parvum (c. parvum) stimulated the t-cell system and had a beneficial effect on the recurrence rate of surgically resected cancers of the uterine cervix. in this paper we report the use of an immunoperoxidase technique to trace c. parvum antigen in the draining lymph nodes. in a guinea pig model the popliteal lymph node was studied after the injection of 70 micrograms of c. parvum in the hind footpad. at 6 h, intact bacter ... | 1985 | 2861687 |
| comparative in-vitro activity of sch 34343 and other antimicrobial agents against anaerobic bacteria. | the activity of sch 34343 was determined against 575 strains of anaerobic bacteria by an agar-dilution method. its activity was compared with that of benzylpenicillin, piperacillin, cefoxitin, imipenem, clindamycin, metronidazole, chloramphenicol, vancomycin, fusidic acid and bacitracin. sch 34343 and imipenem were the most active agents tested. based on these results, sch 34343 appears to be a promising antimicrobial agent for anaerobic infections and warrants further clinical investigations. | 1985 | 2863246 |
| a comparison of the in vivo antibacterial effects of 'opsite', 'tegaderm' and 'ensure' dressings. | three semi-permeable polyurethane dressings, 'ensure', 'opsite' and 'tegaderm', were tested for their ability to prevent the increase in bacterial population under naturally hydrated, occluded volar forearm skin. only 'opsite' inhibited the bacterial multiplication in these tests. it is concluded that in the practical situation, 'opsite' could well confer a greater margin of safety. | 1985 | 2865296 |
| the subunit structure of methylmalonyl-coa mutase from propionibacterium shermanii. | 5'-deoxyadenosylcobalamin-dependent methylmalonyl-coa mutase was purified to homogeneity from propionibacterium shermanii by a simplified procedure. the native enzyme has an apparent mr of 165,000, similar to the enzyme from other sources but larger than previously reported. it consists of two non-identical subunits, of mr 79,000 and 67,000 respectively. the smaller subunit is apparently not a proteolytic fragment of the larger one. the final preparation usually contained some inactive mutase, b ... | 1986 | 2875711 |
| intrapleural and intravenous corynebacterium parvum in patients with resected stage i and ii non-small cell carcinoma of the lung. the ludwig lung cancer study group. | a prospective randomized trial compared the administration of intrapleural plus intravenous corynebacterium parvum (c. parvum) versus placebo in patients with resected stage i and stage ii non-small cell bronchogenic carcinoma. treatment consisted of 7 mg c. parvum injected into the pleural space and 7 mg c. parvum intravenously once between days 6 and 12 postoperatively and 7 mg intravenously every 3rd month during the 1st year. intrapleural administration of 35 cc of saline served as the place ... | 1986 | 2876772 |
| protection of mice against intestinal amoebiasis with bcg, corynebacterium parvum and listeria monocytogenes. | treatment with corynebacterium parvum or bcg, or infection with live listeria monocytogenes was found to protect mice against subsequent infection with entamoeba histolytica. complete protection was obtained in mice treated with 10(7) (colony forming units) of bcg but not with 10(5). partial protection was achieved with 10(6) of bcg. these data provide evidence that non-specific immunity plays an important role in the host defense against amoebic infection. | 1986 | 2880331 |
| mechanism of decline of natural killer cell activity in corynebacterium parvum-treated mice: inhibition by erythroblasts and thy 1.2+ lymphocytes. | injection of (c57bl/6 x dba/2)f1 mice with corynebacterium parvum (cp), iv, resulted in a depression of splenic natural killer (nk) cell activity 7-17 days after treatment. this decline in reactivity was accompanied by an increase in splenic tumor-binding cells and a decrease in cytotoxic tumor-binding cells as evaluated in a single-cell assay. morphologic analysis indicated that the increased tumor-binding cells following cp injection were due to erythroblasts binding to yac-1 tumor cells. with ... | 1987 | 2887687 |
| quantitative measurement of the error in the cryptic stereospecificity of methylmalonyl-coa mutase. | 1. samples of methylmalonyl-coa and (2h3)methylmalonyl-coa were prepared by a combination of chemical and enzymic methods. after ion-exchange chromatography the unlabelled methylmalonyl-coa was pure, the deuterated substance contained 11-12% dephospho-coa derivative. 2. the sample of unlabelled methylmalonyl-coa was incubated in deuterated buffer with catalytic amounts of methylmalonyl-coa mutase, epimerase, and coenzyme b12. the progress of the reaction was monitored directly by 1h-nmr spectros ... | 1987 | 2889598 |
| the error in the cryptic stereospecificity of methylmalonyl-coa mutase. the use of carba-(dethia)-coenzyme a substrate analogues gives new insight into the enzyme mechanism. | a preparation containing 80.0 +/- 0.5% (2rs)-methylmalonyl-carba-(dethia)-coa and 20.0 +/- 0.5% propionyl-carba-(dethia)-coa was reacted in buffered deuterium oxide with catalytic amounts of coenzyme b12, methylmalonyl-coa mutase and methylmalonyl-coa epimerase. the rearrangement of the methylmalonyl-carba-(dethia)-coa to succinyl-carba-(dethia)-coa was monitored by recording 500-mhz 1h-nmr spectra in short time intervals. after reaching equilibrium (approximately equal to 28 min) the products s ... | 1988 | 2895708 |
| evolutionary conservation among biotin enzymes. | | 1988 | 2896195 |
| crystallization and preliminary diffraction data for adenosylcobalamin-dependent methylmalonyl-coa mutase from propionibacterium shermanii. | pink crystals of methylmalonyl-coa mutase from propionibacterium shermanii, a coenzyme b12 (5'-deoxyadenosylcobalamin)-dependent enzyme, have been obtained by the hanging-drop method in two different forms. one form lies in the space group p21, with unit cell dimensions a = 122 a, b = 160 a and c = 90 a, with beta = 104 degrees (1 a = 0.1 nm). there are two alpha beta dimers in the asymmetric unit. the crystals diffract to 3.2 a resolution and are suitable for high resolution x-ray diffraction s ... | 1988 | 2897473 |
| contamination of blood during cardiopulmonary bypass: the effect of antibiotic prophylaxis. | despite antibiotic prophylaxis in cardiac surgery, gram-positive bacteria can be isolated in up to 10% of intraoperative blood cultures. during a prospective randomized trial, blood was collected from the oxygenator at the end of bypass in 58 patients given teicoplanin and in 60 others given flucloxacillin and tobramycin. coagulase-negative staphylococci were cultured from 16 patients given teicoplanin but in only four cases after flucloxacillin and tobramycin (fisher's exact test, p = 0.005). i ... | 1988 | 2905372 |