Publications

TitleAbstractYear
Filter
PMID
Filter
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents. special references to bacteria isolated between july 1995 and june 1996].isolated bacteria from infections in general surgery during the period from july 1994 to june 1995 were investigated in a multicenter study in japan, and the following results were obtained. one hundred and sixty-four strains were isolated from primary infections, and 202 strains were isolated from postoperative infections. from primary infections, anaerobic gram-positive bacteria were predominant, while from post operative infections, aerobic gram-positive bacteria were predominant. among aerob ...19979100076
periodontal bacteria colonizing oral mucous membranes in edentulous patients wearing dental implants.the purpose of this study was to investigate the prevalence of the microbiota on the oral mucosal surfaces and in the peri-implant pocket in edentulous subjects with a past history of periodontitis. twenty edentulous subjects wearing dental implants for at least one year and with a history of periodontitis participated in this study. clinical parameters were assessed (plaque, redness, swelling, peri-implant probing depth [pipd], and bleeding on probing) and microbiological samples were taken (or ...19979100195
[the detection of peptostreptococcus asaccharolyticus and bacillus cereus in clinical materials from a child who died of sepsis with a fulminant clinical course].a case of an acute disease with a rapid clinical course and a fatal outcome in the presence of irreversible toxicoinfectious shock, appearing in two children after the consumption of sheep kidneys, is described. the post mortem examination of the children revealed the presence of hemorrhagic, erosive and necrotic areas on the mucous membrane of the stomach, the duodenum and the upper section of the small intestine. from the material obtained by probing the stomach of one of the children 6 hours ...19969103069
characterization of smooth and rough morphotypes of peptostreptococcus micros.isolation of the smooth (sm) morphotype of peptostreptococcus micros, a suspected oral pathogen, is sometimes accompanied by isolation of a rough (rg) morphotype of p. micros. the rg type readily changes to a sm-like variant (rgsm) in broth culture. sm and rg isolates and rgsm variants were compared to determine whether these three types are the result of phase variation. the rgsm variants resembled the sm morphotype in colony morphology; furthermore, the sm type and the rgsm type did not have t ...19979103622
cervical necrotizing fasciitis as a complication of acute epiglottitis. 19979106089
polymicrobial brain abscess in a patient infected with human immunodeficiency virus.although intracranial mass lesions that occur as a result of infection have commonly been reported in patients infected with human immunodeficiency virus, polymicrobial pyogenic brain abscess has not been described in this setting. we report the first case of a patient with a polymicrobial brain abscess involving streptococcus bovis, fusobacterium necrophorum, peptostreptococcus, and group g streptococcus, and we review the relevant world literature.19979114198
kinetics of folding of the igg binding domain of peptostreptococcal protein l.the kinetics of folding of a tryptophan containing mutant of the igg binding domain of protein l were characterized using stopped-flow circular dichroism, stopped-flow fluorescence, and hd exchange coupled with high-resolution mass spectrometry. both the thermodynamics and kinetics of folding fit well to a simple two-state model: (1) guanidine induced equilibrium denaturation transitions measured by fluorescence and circular dichroism were virtually superimposable. (2) the kinetics of folding/un ...19979116017
tissue penetration of meropenem in patients undergoing gynecologic surgery.the purpose of this study was to assess the tissue-penetrating ability of a new beta-lactam antibiotic, meropenem, in 64 patients undergoing elective gynecologic surgery. patients received a single 500-mg dose intravenously before surgery. plasma and tissue concentrations of meropenem were highest at approximately 1 hour, and good tissue penetration was seen in the variety of specimens evaluated. the median plasma concentration at approximately 1 hour was 13.3 micrograms/ml. the median fluid and ...19979126690
penicillin g and penicillin g-tinidazole treatment of experimentally induced summer mastitis--effect on elimination rates of bacteria and outcome of the disease.two udder hind quarters of ten pregnant heifers were inoculated experimentally with a combination of actinomyces pyogenes, peptostreptococcus indolicus and fusobacterium necrophorum at a dose of 9 x 10(8) colony forming units (cfu) of each species of bacteria. the development of aerobic-anaerobic mastitis was followed-up clinically and through analysis of udder secretion samples. all heifers developed acute clinical mastitis. thick, purulent secretions with foul odour were already present 24 h a ...19949133056
a case of secondary sjögren's syndrome with a swollen thigh. 19979135217
antibacterial activities of tosufloxacin against anaerobic bacteria and the electron micrograph of its bactericidal effects.tosufloxacin, a quinolone, showed a broad antibacterial spectrum against gram-positive and gram-negative bacteria including anaerobic bacteria. tosufloxacin was 4- to 8-fold more active than levofloxacin and ciprofloxacin. the mic90 of tosufloxacin for clinical isolates of bacteroides fragilis, bacteroides vulgatus, bacteroides thetaiotaomicron and peptostreptococcus asaccharolyticus were 0.78, 0.39, 1.56 and 0.39 micrograms/ml, respectively. morphological observation with the scanning and trans ...19979142454
synthesis by an alpha-glucosidase of glycosyl-trehaloses with an isomaltosyl residue.glycosyl-trehaloses with an isomaltosyl residue were synthesized by alpha-glucosidase from aspergillus niger by using maltotetraose as a glucosyl donor and trehalose as the acceptor. the one trisaccharide and two tetrasaccharides formed were isolated by successive column chromatography. the results of an enzymatic digestion, methylation analysis, and 13c-nmr studies indicated that these oligosaccharides were alpha-isomaltosyl alpha-glucoside, alpha-isomaltotriosyl alpha-glucoside and alpha-isoma ...19979145529
microbiology of chronic maxillary sinusitis: comparison between specimens obtained by sinus endoscopy and by surgical drainage.the aerobic and anaerobic microbiology of sinus aspirates obtained during surgery was compared with culture of samples obtained by endoscopy. six patients with chronic maxillary sinusitis were evaluated. polymicrobial flora was found in all specimens (two-to-five isolates/sample). a total of 24 isolates (18 anaerobic, five aerobic and one micro-aerophilic) was obtained from sinus aspirates, and 25 isolates (16 anaerobic and nine aerobic) were found in endoscopic specimens. the predominant organi ...19979152041
organisms isolated from dogs and cats with anaerobic infections and susceptibility to selected antimicrobial agents.to determine the prevalence of obligate anaerobic bacteria in bacterial infections in dogs and cats and susceptibility to selected antimicrobial agents.19979170087
matrix metalloproteinases-1, -3 and -8 and myeloperoxidase in saliva of patients with human immunodeficiency virus infection.human immunodeficiency virus (hiv)-seropositive patients have frequently severe gingival inflammation and/or attachment loss. in addition many infectious diseases affect their periodontium with varying clinical manifestations. matrix metalloproteinases seem to play a key role in physiological periodontal remodelling and pathological tissue destruction. the aim of the present study was to characterize the presence, molecular forms, cellular sources, activities, and relative amounts of fibroblast- ...19969171509
protein degradation by prevotella intermedia and actinomyces meyeri supports the growth of non-protein-cleaving oral bacteria in serum.the proteolytic activities of oral bacteria are thought to play an important role in the aetiology of dental abscesses. bacteria-derived proteases may contribute to tissue destruction, and are likely to impair host defence by degrading immunoglobulins and complement. degraded periodontal tissue and tissue fluid are likely to constitute essential sources of nutrients in the abscess. tissue fluid, which is derived from serum, is rich in protein and poor in carbohydrate, suggesting that breakdown o ...19979178115
periodontal findings in cohen syndrome with chronic neutropenia.radiographic periodontal status and microbiological findings of periodontal pockets in subjects with cohen syndrome are presented in this report. this hereditary disorder causes mental retardation, and neutropenia is one feature of the syndrome. fifteen patients with cohen syndrome and 15 controls matched for age and sex and, as far as possible, according to the degree of mental retardation were examined. alveolar bone loss was evaluated from the panoramic radiographs. two subgingival samples we ...19979182743
microbiology of nonbullous impetigo.our objective was to establish the aerobic and anaerobic microbiology of nonbullous impetigo (ni) in children. we used a retrospective review of clinical microbiology laboratory and patients' records. specimens were obtained from 40 patients with ni lesions and showed bacterial growth. aerobic or facultative anaerobic bacteria only were present in 24 patients (60%), strict anaerobic bacteria only in 5 patients (12.5%), and mixed anaerobic-aerobic flora was present in 11 patients (27.5%). sixty-f ...19979192410
associations of endodontic symptoms and signs with particular combinations of specific bacteria.significant associations have been reported between (a) specific bacterial species isolated from root canals and (b) between individual bacterial species and endodontic symptoms and signs. the prime objective of this study was to determine whether particular combinations of specific bacteria are associated with individual endodontic symptoms and signs. seventy root canals were investigated microbiologically taking care to maintain the viability of obligate anaerobes, which accounted for 64% of t ...19969206427
variations in the susceptibilities of components of the endodontic microflora to biomechanical procedures.the role of the endodontic microflora in pulpal disease and in endodontic treatment failures is well established. thus the need for effective microbial control is one of the important justifications for biomechanical procedures. however the efficacy of this stage of treatment is dependent upon the vulnerability of the involved species, which may not be uniform. the aim of this study was to investigate variations in the susceptibilities of members of the root canal microflora to routine biomechan ...19969206439
the antimicrobial activity of endodontic sealers to anaerobic bacteria.the effect of five root canal sealers and two root canal dressing materials on the growth of three anaerobic bacteria associated with endodontic infections was determined using the agar diffusion inhibitory test. samples of the following endodontic sealers (apexit, ketac-endo, roth sealer, sealapex and tubliseal) and root canal dressing materials (pulpdent and root-cal) were incubated for 48 h with the following anaerobic bacteria: capnocytophaga ochracea, porphyromonas gingivalis and peptostrep ...19969206446
immune response to fusobacterium nucleatum, prevotella intermedia and other anaerobes in children with acute tonsillitis.the number of aerobic and anaerobic bacteria was determined in the saliva of 20 children with acute group a beta-haemolytic streptococcal (gabhs) pharyngo-tonsillitis, and 20 with acute non-gabhs tonsillitis. antibody titres to four gram-negative anaerobic bacilli that reside in the oropharynx (fusobacterium nucleatum, prevotella intermedia, porphyromonas gingivalis, and actinobacillus actinomycetemcomitans) were determined in these and 20 control patients. an average of 8.8 aerobic and anaerobi ...19979222046
time-kill study of the activity of trovafloxacin compared with ciprofloxacin, sparfloxacin, metronidazole, cefoxitin, piperacillin and piperacillin/tazobactam against six anaerobes.a time-kill method was developed to examine the killing kinetics of trovafloxacin, ciprofloxacin, sparfloxacin, metronidazole, cefoxitin, piperacillin and piperacillin/tazobactam against one strain each of bacteroides fragilis, bacteroides thetaiotaomicron, prevotella melaninogenica, fusobacterium mortiferum, peptostreptococcus magnus and clostridium perfringens. solutions and suspensions were prepared inside an anaerobic glove box, using syringes and prereduced broth. bottles were then incubate ...19979222066
occurrence of invading bacteria in radicular dentin of periodontally diseased teeth: microbiological findings.bacterial invasion in roots of periodontally diseased teeth, which has been recently documented using cultural and microscopic techniques, may be important in the pathogenesis of periodontal disease. the purpose of this investigation was to determine the occurrence and the species of invading bacteria in radicular dentin of periodontally diseased teeth. samples were taken from the middle layer of radicular dentin of 26 periodontally diseased teeth. 14 healthy teeth were used as controls. dentin ...19979226388
nmr analysis of the interaction between protein l and ig light chains.protein l is a cell wall protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus. it binds to immunoglobulin (ig) light chains predominantly of the kappa subtype from a wide range of animal species. this binding is mediated by five highly homologous repeats designated as b1-b5, each of which comprises 72 to 76 amino acid residues. the fold of the ig light chain-binding b1 domain of protein l has previously been shown to comprise an alpha-helix packed agains ...19979231896
ovarian vein thrombosis with symptoms prior to term--a case report.a case with ovarian vein thrombosis before delivery is presented. c-reactive protein exceeded 200 mg/l, and the patient was treated for a suspected infection before the true diagnosis was revealed by ct. after aggressive anti-coagulant therapy, a follow-up ct showed complete remission.19979243212
cloning and expression of the two genes coding for l-serine dehydratase from peptostreptococcus asaccharolyticus: relationship of the iron-sulfur protein to both l-serine dehydratases from escherichia coli.the structural genes sdha and sdhb, coding for the alpha- and beta-subunits of the [4fe-4s] cluster containing l-serine dehydratase from peptostreptococcus asaccharolyticus, have been cloned and sequenced. expression of modified sdhb together with sdha in escherichia coli led to overproduction of active his6-tagged l-serine dehydratase. e. coli mew22, deficient in the l-serine dehydratase l-sd1, was complemented by this sdhba construct. the derived amino acid sequence of sdhba shares similaritie ...19979244285
separation method of igg fragments using protein l.protein l (igg kappa-chain-binding bacterial protein) showed a precipitate line(pseudo-immuno-reaction) with igg and f(ab')2 fragment, but did not show any line with the fab fragment, the fc fragment and free kappa-chains in the micro-ouchterlony method. the igg and fab fraction obtained from pa-pain-digested igg (from the sera of patients with chronic thyroiditis), followed by protein a-sepharose, were separated by protein l-sepharose affinity chromatography. the unbound fraction (uf) consisted ...19979246561
microorganisms in vaginal fluid from women in prolonged pregnancy.in order to compare the vaginal microflora of women in prolonged pregnancy with that of women who delivered at term, samples for quantitative aerobic and anaerobic microbiological culture were collected from 100 women at 42 weeks of gestation and from 60 women at term. the occurrence of lactobacilli-dominated flora was similar in women at term and women with prolonged pregnancy. however, non-hydrogen-peroxide-producing lactobacilli (p < 0.01) were significantly more common and peptostreptococci ...19979251948
beta-lactamase producing bacteria in adult periodontitis.in 23 untreated adult periodontitis patients, the occurrence of beta-lactamase producing periodontal bacteria was determined. in addition to non-selective isolation media, selective isolation and growth of beta-lactamase positive subgingival bacterial species was carried out on blood agar plates supplemented with amoxicillin and plates with amoxicillin+clavulanic acid. porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, peptostreptococcus micros, fusobacterium ...19979266340
effect of the addition of peptostreptococcus productus atcc35244 on the gastro-intestinal microbiota and its activity, as simulated in an in vitro simulator of the human gastro-intestinal tract.peptostreptococcus productus atcc35244, a reductive acetogenic strain, was added daily over 9 successive days to the fourth vessel (ascending colon) of the shime, a six-stage reactor system simulating the in vivo continuous culture conditions of the human gastro-intestinal tract. final numbers of organisms (cfu)/ml reactor contents (c) were attained such that log10c = 6.9 +/- 0.1. the addition caused the ch4 production to decrease below the detection limit while total gas and co2 production in t ...19979274052
use of the isolator 1.5 microbial tube for culture of synovial fluid from patients with septic arthritis.synovial fluid specimens obtained from patients with arthritis were plated onto solid media (conventional cultures) or inoculated into an isolator 1.5 microbial tube (isolator cultures), and the yield and time to detection of organisms were compared. overall, 144 specimens obtained from 137 patients were processed, and 31 (21.5%) cultures obtained from 29 patients were positive by at least one method. staphylococcus aureus was isolated from 12 patients, streptococcus pneumoniae and kingella king ...19979276427
peptostreptococcal myonecrosis of extraperitoneal origin--a life-threatening complication of pelvic ring disruption.this report illustrates the clinical and pathological findings of anaerobic streptococcal myonecrosis following extraperitoneal rectal injury in a 28-year-old patient with traumatic pelvic ring disruption. four days following admission, the patient underwent laparotomy, debridement and drainage of the left-sided abdominal wall and presacral space for gross surgical emphysema and streptococcal anaerobic myonecrosis. the specificity of this infection and its impact on the diagnosis and treatment o ...19979283248
increased susceptibility of rag-2 scid mice to dissemination of endodontic infections.specific immunity has been implicated in the pathogenesis of periapical lesions, although the extent to which these mechanisms are actually involved in either protection or destruction of the pulp-periapex complex is yet to be established. to investigate this question we compared periapical-lesion pathogenesis in rag-2 severe combined immunodeficient (scid) mice with immunocompetent control mice following surgical pulp exposure. in order to equalize the bacterial challenge, an infection protocol ...19979284152
recently described clinically important anaerobic bacteria: taxonomic aspects and update.a new method of identifying bacteria, phylogenetic 16s rrna sequencing, has led to major reorganizations among most genera of anaerobic bacteria. the pigmented prevotella species now comprise seven species including p. nigrescens and p. tannerae; p. intermedia/p. nigrescens-like organisms await inclusion. the former mitsuokella dentalis and hallella seregens were transferred to prevotella as one species, p. dentalis. p. enoeca is a new nonpigmenting prevotella. the genus porphyromonas currently ...19979310640
dental implant infections.dental implants provide a restorative tool to support crowns, bridge abutments, and removable dentures. osseointegrated implants are titanium posts that are surgically implanted in alveolar bone. a tight immobile bond (osseointegration) forms between bone and titanium, and prosthetic and restorative fixtures are attached to the implants. titanium implants differ from natural teeth, which may make them more susceptible to mechanical stress. a small proportion of implants are not successful and ma ...19979310682
bilateral anaerobic empyemas complicating infectious mononucleosis. 19979315821
in-vitro activity of spiramycin and metronidazole alone or in combination against clinical isolates from odontogenic abscesses.one hundred and forty-eight isolates of bacteria from 20 intraoral odontogenic abscesses were tested for their susceptibility to spiramycin and metronidazole alone or in combination. all isolates, except rothia spp. (one), enterococcus avium (three), haemophilus parainfluenzae (one) and staphylococcus aureus (one) were sensitive to spiramycin and/or metronidazole. among the anaerobes, spiramycin as well as metronidazole showed good antimicrobial activity against species of prevotella, eubacteriu ...19979338486
the aerobic and anaerobic bacteriology of perirectal abscesses.the microbiology of perirectal abscesses in 144 patients was studied. aerobic or facultative bacteria only were isolated in 13 (9%) instances, anaerobic bacteria only were isolated in 27 (19%) instances, and mixed aerobic and anaerobic flora were isolated in 104 (72%) instances. a total of 325 anaerobic and 131 aerobic or facultative isolates were recovered (2.2 anaerobic isolates and 0.9 aerobic isolates per specimen). the predominant anaerobes were as follows: bacteroides fragilis group (85 is ...19979350771
prospective study of bacteremia rate after elastic band ligation and sclerotherapy of esophageal varices in patients with hepatosplenic schistosomiasis.esophageal band ligation is considered to be as efficient as endoscopic sclerotherapy, with a lower complication rate, including bacteremia. there are few studies comparing the two methods. the aim of this study was to compare the incidence of bacteremia after both treatments in patients with portal hypertension secondary to schistosomiasis.19979351034
distribution and mobility of the tetracycline resistance determinant tetq.we tested 34 american type culture collection (atcc) and 168 clinical bacterial isolates, from the human urogenital and oral tracts and streptococci isolated from cows with mastitis, for the presence of the tetq gene using a polymerase chain reaction (pcr) assay and dna-dna hybridization. the identities of pcr products were confirmed by southern blot hybridization of whole-cell dna. eleven of the atcc strains were positive for tetq, including five bacteroides spp., five prevotella spp. and a sin ...19979372425
metastatic bacterial endophthalmitis. a report of four cases all leading to blindness.to draw attention to the rare but severe entity of endophthalmitis as encountered due to metastatic spread of bacteria.19979374263
taxonomic update and clinical significance of species within the genus peptostreptococcus. 19979376474
coronal leakage of mixed anaerobic bacteria after obturation and post space preparation.the purpose of this study was to assess bacterial leakage of a mixed anaerobic community of organisms in obturated canals after post space preparation.19979377197
characterization of the free energy spectrum of peptostreptococcal protein l.native state hydrogen/deuterium exchange studies on cytochrome c and rnase h revealed the presence of excited states with partially formed native structure. we set out to determine whether such excited states are populated for a very small and simple protein, the igg-binding domain of peptostreptococcal protein l.19979377710
[site-specific microbiological tests and the diagnostic significance-- dental and oral infections]. 19979379527
[antimicrobial activities of clarithromycin against recent obtained clinical isolates].in order to evaluate antimicrobial activities of clarithromycin (cam), minimum inhibitory concentrations (mics) of cam and control drugs were determined against clinical isolates that were obtained from outpatients in 1994 and 1996. the results are summarized as follows; 1. it was not showed that cam-resistant strains were increasing among staphylococcus spp., beta-streptococci, moraxella subgenus branhamella catarrhalis, haemophilus influenzae, bordetella pertussis, campylobacter jejuni subsp. ...19979394238
[trend of bacterial meningitis in children over a 14 year period (1981 through 1994) in japan--an analysis based on studies in 27 institutions].we observed 266 children with purulent meningitis in 27 institutions in japan during the 14 years from 1981 on dividing these years into 3 periods, 1981-1985, 1986-1990 and 1991-1994, and studied the trend of causative organisms identified in 254 among the 266 patients. their ages were less than 3 months after birth in 50 children and 3 months or older in 216: there were 141 boys and 125 girls. the causative organisms were h. influenzae in 134 patients and s. pneumoniae in 50, most of them being ...19979394553
evaluation of the bbl crystal anaerobe identification system.the bbl crystal anaerobe (anr) identification system was evaluated, and the results were compared with those from conventional anaerobic methods. we tested 322 clinically significant anaerobic bacteria according to the manufacturer's instructions. the system identified correctly 286 of 322 (88.8%) of the anaerobic bacteria tested. of these, 263 of 322 (81.7%) were identified correctly on initial testing and 49 were identified correctly only to the genus level; on repeat testing, 23 of 49 (46.9%) ...19979399517
macrolides resistance of common bacteria isolated from taiwan.to determine the susceptibility to macrolides of common pathogenic bacteria isolated from taiwan, the in vitro activities of erythromycin, roxithromycin, azithromycin, clarithromycin, and dirithromycin were tested against 492 clinical isolates of eight different bacteria, collected from the national taiwan university hospital. the results showed high minimum inhibitory concentrations (mics) against most of the tested bacteria. the mic90s for staphylococcus aureus (both methicillin-resistant and ...19959407220
[meropenem: microbiologic perspective].meropenem is a beta-lactamic carbapenem derived from thienamycin and is structurally characterized by the presence of a beta-methyl group in position c1 which confers stability to the molecule versus renal dehydropeptidase 1 (dhp-1), thereby making the coadministration of an enzyme inhibitor unnecessary. its esterochemical configuration of the lateral chain in c2 (dimethyl carbomoilpyrrolidenethium) increases the activity versus gram negative bacteria (enterobacteria and pseudomonas) and moreove ...19979410064
[anti-anaerobic activity of carbapenems].the critical concentrations of sensitivity and resistance for meropenem versus anaerobic bacterias were analyzed. it is demonstrated the meropenem is a powerful inhibitor of the microorganisms of the bacteroides groups (mic90 < 1 mg/l), prevotella > porphyromonas, fusobacterium, veillonella, clostridium perfringens is inhibited at a mic < 0.06 mg/l. the mic of meropenem versus c. difficile is 2 mg/l. they are also highly susceptible to the propionibacterium, peptostreptococcus and peptococcus ty ...19979410076
[aerobic and anaerobic bacterial flora in chronic sinusitis in adults].the aim of the study was to analyse microbiologically samples obtained from 30 patients aged from 21 to 73 years treated for chronic sinusitis. aerobic bacteria only were isolated in 16 patients (53%), and anaerobic organisms only in 5 patients (17%). mixed aerobic and anaerobic isolates were recovered from 9 patients (30%). the isolated aerobic bacteria were as follows: streptococci from the species streptococcus salivarius, streptococcus anginosus, streptococcus group c, streptococcus sanguis, ...19979411078
[microflora of periodontal pockets in advanced periodontitis].the aim of study was the evaluation of periodontal pockets microflora in patients with advanced periodontitis. from each subject 16-20 samples were taken using paper points. pooled sample after 60 s. mixing was serially diluted in reduced bhi. for total cell counts and for the isolation of black pigmented anaerobes brucella agar supplemented with 5% sheep blood, hemin, menadione, with and without kanamycin-vancomycin mixture and bm agar plates were used. for isolation of a. actinomycetemcomitans ...19979411079
contrasting roles for symmetrically disposed beta-turns in the folding of a small protein.to investigate the role of turns in protein folding, we have characterized the effects of combinatorial and site-directed mutations in the two beta-turns of peptostreptococcal protein l on folding thermodynamics and kinetics. sequences of folded variants recovered from combinatorial libraries using a phase display selection method were considerably more variable in the second turn than in the first turn. these combinatorial mutants as well as strategically placed point mutants in the two turns h ...19979417937
[right-sided infective endocarditis with ventricular septal defect].two patients underwent surgical treatment for right-sided infective endocarditis with ventricular septal defect. in both cases, blood cultures showed peptostreptococcus, and the operation was performed at non-active phase after antibiotics therapy. the case 1 was a 7-year-old girl who was observed a vegetation on the chorda of the anterior paillary muscle by echocardiography. the defect was directly closed and the vegetation was excised. the case 2 was 22-year-old female who had been diagnosed o ...19949423075
anaerobic meningitis due to peptostreptococcus species: case report and review.we describe a patient with postsurgical anaerobic meningitis due to peptostreptococcus magnus. in cases of meningitis associated with peptostreptococcus species reported in the literature, the most common predisposing factors are meningorectal fistulae and head-and-neck surgery. most patients respond well to appropriate antimicrobial therapy. surgical intervention may be required in some instances.19979431395
microbiology of nosocomial sinusitis in mechanically ventilated children.to assess the bacteriology of nosocomial sinusitis in mechanically ventilated children.19989440778
periodontitis patient and the spouse: periodontal bacteria before and after treatment.suspected periodontal pathogens can be transmitted between spouses. the treatment response may be unsuccessful in periodontitis patients, if the spouse harbors these bacteria. the aim of the present 6-month follow-up study was to clarify whether the microbiological treatment outcome of periodontitis patients is related to the detection of suspected periodontal pathogens in the saliva of the spouse. 10 patients with advanced periodontitis and their spouses were included in the study. the patients ...19979442426
chronic sinusitis in children.in children, sinusitis is a common, generally uncomplicated, and self limiting disease which drops with age. the diagnosis is difficult because of relatively non specific signs and symptoms that overlap with viral upper respiratory infection and allergy. plain paranasal sinus radiographs are not adequate, in determining the extent of involvement in recurrent or chronic sinusitis and so ct scan has become the standard. viral illness appears to be the most common predisposing factor. immune defect ...19979444376
in vitro and in vivo antibacterial activities of a new carbapenem bo-2727 for use in obstetrics and gynecology.the antibacterial activity of bo-2727 against 100 clinical isolates of streptococcus agalactiae (20 strains), escherichia coli (20 strains), peptostreptococcus magnus (20 strains), bacteroides fragilis (20 strains) and prevotella bivia (20 strains) was compared with those of imipenem, panipenem, meropenem, biapenem and ceftazidime (caz). minimal inhibitory concentrations (mics) for 50% of tested strains (mic50s) of bo-2727 against s. agalactiae, e. coli, p. magnus, b. fragilis and p. bivia were ...19989444403
bacteriology of inflamed and uninflamed epidermal inclusion cysts.to determine whether inflamed and uninflamed epidermoid cysts differ in the number and/or type of bacteria inhabiting them.19989449909
gram-positive anaerobic cocci.gram-positive anaerobic cocci (gpac) are a heterogeneous group of organisms defined by their morphological appearance and their inability to grow in the presence of oxygen; most clinical isolates are identified to species in the genus peptostreptococcus. gpac are part of the normal flora of all mucocutaneous surfaces and are often isolated from infections such as deep organ abscesses, obstetric and gynecological sepsis, and intraoral infections. they have been little studied for several reasons, ...19989457430
aerobic, anaerobic and fungal burn wound infections.one hundred and twenty-seven patients were studied prospectively for aerobic, anaerobic and fungal burn wound infections. all cases yielded organisms on culture. a total of 377 isolates were recovered (239 aerobes, 116 anaerobes and 22 fungi). aerobic bacteria alone were present in 49 patients (38.6%). anaerobic bacteria alone were present in four patients (3.2%). candida sp. alone was present in one patient (0.8%). mixed aerobic and/or anaerobic bacteria and/or fungi were present in 73 patients ...19979457609
purulent pericarditis secondary to candida parapsilosis and peptostreptococcus species.purulent pericarditis secondary to candida pericarditis is a rare but lethal infection. the first case of pericardial infection by candida parapsilosis in combination with peptostreptococcus species in an immunocompetent nondebilitated individual is reported. urgent pericardiectomy was required after pericardial drainage failed to stop symptoms of tamponade. the etiology, microbiology and treatment of this unusual condition are reviewed.19989487278
[non-sporeforming anaerobic bacteria in bronchoalveolar lavage fluid of patients with pneumonia during the course of lung cancer].the aim of this study was to assess the occurrence of non-sporeforming anaerobes in bronchoalveolar lavage in patients with pneumonia coexisting with lung cancer. material consisted of 40 patients with lung cancer. bronchoalveolar lavage was performed before administration of antimicrobial treatment and obtained material was cultivated in anaerobic and aerobic conditions. quantitative assessment of anaerobic bacteria and their susceptibility to common antimicrobial agents were also performed. pe ...19979489438
periapical abscesses: causal bacteria and antibiotic sensitivity.the purpose of this study was to investigate the aerobic and anaerobic flora of periapical abscesses and evaluate their susceptibility to various antibiotics. in 52 patients, with a diagnosis of periapical abscesses, pus cultures were taken. forty-two aerobes and 122 anaerobes were revealed, with 2 or more than 2 anaerobic strains isolated in 36 patients. infections were purely aerobic in 6%, purely anaerobic in 17% and mixed in 75%, while in 2% of the specimens there was no growth of microorgan ...19979491841
dacryocystitis caused by anaerobic bacteria in the newborn. 19989493822
microbial complexes in subgingival plaque.it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ...19989495612
anaerobic bacteria in orofacial abscesses.obligate anaerobic bacteria were cultured from 15 orofacial abscesses. bacteroides species constituted 43 per cent, clostridium 21 per cent, fusobacterium 14 per cent, peptostreptococci 11 per cent, peptococci 7 per cent and veillonella 4 per cent of the isolates. this study confirms the polymicrobial nature of orofacial infections. sensitivity to antibiotics was unpredictable. clostridium difficile, clostridium tetani, peptostreptococcus productus and veillonella parvula showed resistance to so ...19939508939
gram-positive resistance: challenge for the development of new antibiotics.the incidence of infections caused by multidrug-resistant gram-positive organisms is increasing despite advances in antibacterial therapy over the last 20 years. as the pathogens causing these infections are frequently resistant to most currently available antibacterials, they are extremely difficult to treat. problematic pathogens include strains of streptococcus pneumoniae resistant to beta-lactams and macrolides, viridans group streptococci resistant to beta-lactams and aminoglycosides, enter ...19979511055
[actinobacteriosis of paranasal sinuses].on the base the review of literature and own case the authors presents diagnostic difficulties and therapy of paranasal sinuses actinobacteriosis. actinobacteriosis of sinuses is very rare case and diagnostic analysis based on mycologic and histopathologic investigations. in our case sinuses actinobacteriosis orbital abscess complication has been noted. antibiotic therapy and surgical treatment have been used in this case.19979518322
microbiology of necrotizing fasciitis associated with omphalitis in the newborn infant.the purpose of this study was to report the aerobic and anaerobic microbiology of periumbilical necrotizing fasciitis in newborn infants.19989527941
aerobic and anaerobic infection associated with malignancy.the goal of this work was to study the microbiology and clinical characteristics of patients with infections associated with malignancy treated over a period of 17 years. a total of 668 specimens were obtained from 605 patients. the malignancies include 224 hematological malignancies and 381 nonhematogenic malignancies. anaerobic bacteria only were isolated in 201 (30%) specimens, aerobic bacteria in 226 (34%), mixed aerobic-anaerobic bacteria in 231 (35%) and candida spp. in 10 (1%). a total of ...19989540171
a microbiological study of pre- and postoperative apicoectomy sites.there is little information on the microbiology of periapical lesions, and no data on the residual microbial flora in the periapex, if any, after apicoectomy procedures. hence, 64 patients treated by apicoectomy procedures were prospectively studied to assess the bacterial flora in the periapex and to evaluate the residual bacteria in postoperative apicoectomy sites. of the 64 lesions studied, 14 (22%) were sterile and 50 (78%) yielded bacteria preoperatively. bacteria could be recovered from 28 ...19979552822
secondary bacterial infection in ghanaian patients with scabies.from 110 patients with secondarily infected scabies lesions, 105 bacteria consisting of 66 aerobes and 39 anaerobes were isolated. a mixture of aerobic and anaerobic bacteria was present in 15 (13.6%). the predominant aerobic and anaerobic bacteria were staphylococcous aureus 39.1% and pepostreptococcus spp. 14.2% respectively. organisms that resided in the mucus membranes close to or in contact with the lesions predominated in those infections. most organisms were recovered from the finger and ...19979557448
aerobic and anaerobic microbiology of dacryocystitis.to investigate the aerobic and anaerobic microbiology of dacryocystitis.19989559743
the sequences of small proteins are not extensively optimized for rapid folding by natural selection.the thermodynamic stabilities of small protein domains are clearly subject to natural selection, but it is less clear whether the rapid folding rates typically observed for such proteins are consequences of direct evolutionary optimization or reflect intrinsic physical properties of the polypeptide chain. this issue can be investigated by comparing the folding rates of laboratory-generated protein sequences to those of naturally occurring sequences provided that the method by which the sequences ...19989560214
aerobic and anaerobic microbiology of retroperitoneal abscesses.the aerobic and anaerobic microbiology of various types of retroperitoneal abscesses was studied by review of the clinical and laboratory data for 161 patients treated between 1974 and 1990 for such abscesses. these included 109 anterior, 8 posterior, 21 retrofascial, and 23 pelvic retroperitoneal abscesses. a total of 472 organisms (2.9 isolates/specimen)--204 aerobic and facultative (1.3/specimen), and 268 anaerobic (1.7/specimen)--were recovered. aerobes only were recovered from 34 abscesses ...19989564479
supernatant cytotoxicity and proteolytic activity of selected oral bacteria against human gingival fibroblasts in vitro.the purpose of this study was to determine if endodontic bacterial act in vitro on human gingival fibroblast functions via extracellular products. the bacteria used were prevotella nigrescens, capnocytophaga ochracea, peptostreptoccocus micros and actinobacillus actinomycetemcomitans. supernatants were collected from bacterial cultures at the beginning of the stationary phase when their density was similar. toxins that inhibited fibroblast proliferation were found in all culture supernatants of ...19989569986
ammonia-hyperproducing bacteria from new zealand ruminants.pasture-grazed dairy cows, deer, and sheep were tested for the presence of ammonia-hyperproducing (hap) bacteria in roll tubes containing a medium in which tryptone and casamino acids were the sole nitrogen and energy sources. colonies able to grow on this medium represented 5.2, 1.3, and 11.6% of the total bacterial counts of dairy cows, deer, and sheep, respectively. a total of 14 morphologically distinct colonies were purified and studied further. restriction fragment length polymorphisms of ...19989572953
effect of red blood cells on the growth of porphyromonas endodontalis and microbial community development.establishment of a microbial community in the root canal system depends on numerous factors, of which nutrient availability may be one of the most important. we hypothesized that the presence of red blood cells or hemoglobin in this environment could cause shifts in microbial composition of communities, resulting in organisms such as porphyromonas endodontalis becoming more dominant. an in vitro model system using mixed, batch cultures was performed with the bacteria p. endodontalis, fusobacteri ...19989573801
subgingival microbiota of shallow periodontal pockets in individuals after head and neck irradiation.this study aimed at investigating the subgingival plaque microorganisms of shallow pockets (< or = 5 mm) in subjects who previously received irradiation in the head and neck region for treatment of nasopharyngeal carcinoma. direct microscopy and anaerobic culture were used. subgingival paper point samples were taken from 6 tooth-sites (one/sextant) per subject for direct microscopy (n = 108). another set of paper points was taken from the deepest of the previously selected sites (one per subject ...19989573815
prevalence of peptostreptococcus micros morphotypes in patients with adult periodontitis.the prevalence of the smooth and rough colonial morphotypes of peptostreptococcus micros was examined with culture technique in 123 patients with adult periodontitis (age 24-68 years). of all subgingival samples, 91% contained the smooth morphotype of p. micros. the smooth morphotype constituted a mean percentage of the total anaerobic viable biota of 6.0%, with a range of 0.02-35.7%. of these samples, 49% contained colonies of the rough morphotype as well, with a mean percentage of the total an ...19989573826
infected stingray injury. 19989576395
tracheal botryomycosis in a patient with tracheopathia osteochondroplastica.tracheal botryomycosis has never been reported in the literature. a 68 year old man presented with progressive dyspnoea and an exophytic mass below the vocal cords was found by bronchoscopy. pathological study of the mass showed tracheal botryomycosis probably associated with peptostreptococcus spp infection. the patient was given intravenous penicillin for six weeks. tracheal obstruction necessitated tracheal resection and the surgical specimen revealed typical findings of tracheopathia osteoch ...19989577526
aerobic and anaerobic bacteriology of otorrhea associated with tympanostomy tubes in children.the microbiology of in 55 ear aspirates obtained from 34 children with chronic otorrhea was studied. aspiration of the middle ear exudate was done immediately following removal of tympanostomy tube (tt). the middle ear aspirates and swab specimens of the external auditory canals were cultured for aerobic and anaerobic bacteria. sixty-five isolates were recovered only from the middle ears, 73 only from the external ear canals, and 73 were present at both sites. analysis of the 138 middle ear isol ...19989583788
[subacute infectious endocarditis in prosthetic valve by peptostreptococcus micros]. 19989586375
bacterial interference in the nasopharynx following antimicrobial therapy of acute otitis media.the effect on the nasopharyngeal bacterial flora of therapy for 10 days with co-amoxiclav or cefprozil was studied in 50 children with acute otitis media. before therapy, potential pathogens (streptococcus pneumoniae, haemophilus influenzae and moraxella catarrhalis) were isolated in 14 (56%) of those treated with co-amoxiclav and 15 (60%) of those treated with cefprozil. following therapy, the reduction in the number of these pathogens was the same in the two groups. however, differences betwee ...19989598781
aminopeptidase activities in peptostreptococcus spp. are statistically correlated to gelatin hydrolysis.one hundred peptostreptococcus isolates from five species were assessed for their ability to hydrolyze gelatin. most peptostreptococcus magnus (95.8%) and peptostreptococcus micros isolates (79.0%) hydrolyzed gelatin in contrast to peptostreptococcus asaccharolyticus (8.0%), peptostreptococcus anaerobius (10.0%), and peptostreptococcus prevotii isolates (16.7%). gelatin hydrolysis in peptostreptococcus magnus and peptostreptococcus micros isolates correlated (r = 0.80; p = 0.0019) with more amin ...19989606913
partial purification of an iron-dependent l-serine dehydratase from clostridium sticklandii.an oxygen-sensitive and highly unstable l-serine dehydratase was partially purified from the gram-positive anaerobe clostridium sticklandii. the final active preparation contained five proteins of 27, 30, 44.5, 46, and 58 kda as judged by sds-page. the n-terminal sequence of the 30 kda subunit showed some similarity to the alpha-subunits of the iron-containing l-serine dehydratases from clostridium propionicum and peptostreptococcus asaccharolyticus. oxygen-inactivated l-serine dehydratase from ...19989637015
osteomyelitis of the fibula due to septic embolism: a rare complication of a graft enteric erosion. case report.we report the case of a 70-year-old female who experienced a graft enteric erosion (gee) three years after a right aorto-femoral bypass. she was treated by right axillo-femoral bypass, removal of the aortic prosthesis and direct aortic suture. the duodenum was stitched up and separated from the aorta by omentoplasty. bacteriological culture of the removed graft identified escherichia coli and peptostreptococcus. antibiotic therapy consisting of amoxicillin and gentamycin was given for seven days ...19989639000
aerobic and anaerobic microbiology of infections after trauma in children.to review the recovery of aerobic and anaerobic bacteria from infections after trauma in children over a 20 year period.19989639177
development of an anaerobic bacterial leakage model.the majority of bacteria associated with infections of endodontic origin are strict anaerobes. the purpose of this study was to develop an endodontic microleakage model using strict anaerobic bacteria in a two-chamber system. nine species of anaerobic bacteria were tested for viability and detection by either turbidity or color change of the broth. a survey of ph chromogenic substrates revealed that bromcresol purple (ph 5.2 = yellow, ph 6.8 = purple) could be used as a chromogenic indicator to ...19989641124
endodontic pathogens stimulate monocyte chemoattractant protein-1 and interleukin-8 in mononuclear cells.microbial infection of the dental pulp leads to the recruitment of leukocytes and the formation of lesions of endodontic origin. the chemokines interleukin-8 (il-8) and monocyte chemoattractant protein-1 (mcp-1) are relatively specific chemoattractants for neutrophils and monocytes, respectively. in the present studies, peripheral blood mononuclear cells were stimulated by streptococcus mutants, porphyromonas endodontalis, and peptostreptococcus anaerobius, which are associated with lesions of e ...19989641137
[antimicrobial activity of macrolides against clinical isolates].antimicrobial activity of 6 macrolides was determined using a micro-broth dilution method, against 535 clinical isolates of 22 species, which were isolated in 1996 from 325 facilities in japan. results were as follows. 1. in general, antimicrobial activities of 14-membered macrolides were higher than those of 16-membered macrolides. the antimicrobial activities of 14-membered macrolides were in the order of clarithromycin (cam), erythromycin (em), roxithromycin (rxm). among 16-membered macrolide ...19989644599
aerobic and anaerobic microbiology of chronic venous ulcers.the role of bacteria in the pathogenesis of chronic venous leg ulcers (cvlu) is unclear. the objective of the study was to establish the aerobic and anaerobic bacteriology of cvlu.19989646126
microbiology of cervical lymphadenitis in adults.the microbiology of needle aspirates from 40 inflamed cervical lymph glands was studied for aerobic and anaerobic bacteria, fungi and mycobacteria. forty-two bacterial, 11 mycobacterial and six fungal isolates were isolated. aerobic bacteria only were recovered in 11 (27.5%), anaerobes alone in five (12.5%) and mixed aerobic and anaerobic bacteria in seven (17.5%). mycobacterium sp. were recovered in 11 (27.5%) and fungi in six (15%). the recovery of anaerobes was associated with dental infectio ...19989655225
comparative in vitro activity of bay 12-8039 and five other antimicrobial agents against anaerobic bacteria.the in vitro activity of bay 12-8039 against 360 anaerobic clinical isolates was determined by the agar dilution method and compared to that of five other antimicrobial agents. bay 12-8039 and imipenem were the most active agents tested. the following mic90 values were determined for bay 12-8039: peptostreptococcus spp. (50 isolates), 1 mg/l; propionibacterium acnes (30 isolates). 0.25 mg/l; clostridium perfringens (30 isolates), 0.5 mg/l; clostridium difficile (50 isolates), 2 mg/l; bacteroides ...19989665302
semiquantitative bacteriology of closed odontogenic abscesses.pus samples from twenty-three dentoalveolar abscesses were collected by needle aspiration and examined by direct inoculation technique using 6 different aerobic and anaerobic agar plates. for aerobic culture, sheep blood agar and chocolate agar (kyokuto pharmaceutical co., tokyo, japan) were used. for anaerobic culture, four different media, 1) brucella hk agar with hemolyzed rabbit blood and defibrillated sheep blood, 2) paramomycin-vancomycin brucella hk agar with hemolyzed rabbit blood, 3) ph ...19989667143
Displaying items 1201 - 1300 of 2255