| measles-associated diarrhoea in northeastern thailand. | a significant proportion of the illness and death of diarrhoeal diseases in the developing world is estimated to be due to the diarrhoea associated with measles. during february 1983-january 1984 a prospective study of measles in a hospital in northeastern thailand was conducted. a total of 550 cases of measles were studied. diarrhoea was the most frequent complication of measles, occurring in 233 cases (42.4%). the largest proportion (46.2%) of cases with diarrhoea occurred in may-july. childre ... | 1986 | 3738607 |
| effect of training on plasma anabolic and catabolic steroid hormones and their response during physical exercise. | | 1986 | 3744643 |
| [clinical aspects of acute intestinal infection caused by vibrio parahaemolyticus]. | | 1986 | 3750950 |
| a reappraisal of the complement fixation test using soluble mycobacterium avium antigen for the detection of m. paratuberculosis infection in cattle. | serums from 263 cattle suspected of having paratuberculosis on the basis of clinical signs, were tested for antibodies to mycobacterium paratuberculosis with a complement fixation test (cft) employing a heat extracted, soluble m. avium antigen. microscopic examination confirmed that 172 (65.4%) clinically affected animals had paratuberculosis, the remainder being disease-free. the specificity and sensitivity of the cft was 92.3% and 74.4% respectively. phenol treatment of serums before testing w ... | 1986 | 3753338 |
| cartilage-inducing factor-a. apparent identity to transforming growth factor-beta. | comparison of the sequence of the n-terminal 30 amino acids of cartilage-inducing factor-a (cif-a) from bovine demineralized bone with the corresponding sequence of human transforming growth factor-beta (tgf-beta) revealed 100% identity. furthermore, cif-a stimulated normal rat kidney fibroblasts to become anchorage-independent and form colonies in soft agar (in the presence of epidermal growth factor) in a manner similar to tgf-beta. similarly, tgf-beta from human platelets induced rat muscle m ... | 1986 | 3754555 |
| cadmium toxicity in kidney cells. resistance induced by short term pretreatment in vitro and in vivo. | epithelial cells from the kidney were freshly isolated from rats pretreated by daily subcutaneous doses of cdcl2 in vivo (0.5-2 mg cd/kg x 5). such cells were incubated in vitro in media with different concentrations of cadmium chloride (0-200 micrograms cd/ml). there was no inhibition of cell growth in such cells. however, in cells isolated from non-treated rats, in vitro exposure to the same concentrations of cdcl2 caused a dose dependent decrease in viability. when cells, isolated from non-tr ... | 1986 | 3754681 |
| stabilization of intracellular recordings in the cat spinal cord using high-frequency ventilation. | stabilization of intracellular recordings by a substantial reduction of respiratory motion may be accomplished by the use of high-frequency ventilation (600-800 breaths/min). the technique is a simple one and enables maintenance of relatively constant blood gas tensions. although the improved stability was demonstrated in experiments performed on alpha motoneurones in the thoracic spinal cord, it is anticipated that a reduced respiratory movement should benefit recordings in other spinal and sup ... | 1986 | 3756629 |
| the effect of c1 spinal cord transection or bilateral adrenal vein ligation on thioridazine-induced arrhythmia and death in the cat. | the phenothiazine thioridazine 1 mg/kg/min was infused intravenously into three groups of cats: (1) thioridazine alone (n = 5), (2) after bilateral adrenal ligation (n = 4), and (3) after spinal cord section at the atlanto-occipital junction (c1; n = 6). the times to arrhythmia and death with thioridazine alone were 47.8 +/- 7.8 and 72.8 +/- 5.6 minutes respectively. after bilateral adrenal ligation, arrhythmia and death occurred at 41.1 +/- 5.2 and 53.1 +/- 5.8 minutes, respectively, which show ... | 1986 | 3760244 |
| [hemodynamic effects of naloxone in man in brain death]. | | 1986 | 3766888 |
| evaluation of methods for enumeration of vibrio parahaemolyticus from seafood. | the efficiency of several enrichment broths in recovering vibrio parahaemolyticus inoculated into fish homogenates was studied. recovery by the most probable number technique was very low in all the broths, while direct plating on thiosulfate citrate bile salt sucrose agar yielded better recovery. a decrease in the enrichment time to 8 from 18 h did not improve recovery. at concentrations exceeding 2.5 micrograms/ml, polymyxin was inhibitory to v. parahaemolyticus. | 1986 | 3767361 |
| functional coupling between transient declines in blood glucose and feeding behavior: temporal relationships. | to assess the strength and time course of the functional coupling between transient declines in blood glucose and meal initiation, access to food was prevented throughout declines followed by restoration of access to food when glucose returned to baseline. neither preventing access to nor the absence of food affected the time course of the decline in blood glucose and the latency to food seeking behavior. when access to food was restored six to eight minutes after the blood glucose returned to b ... | 1986 | 3768746 |
| [regeneration of changes induced by a single dose of bromhexine in the epithelium of the respiratory tract]. | | 1986 | 3769026 |
| role of glucagon in splanchnic hyperemia of chronic portal hypertension. | the role of glucagon as a blood-borne mediator of the hyperdynamic circulation associated with chronic portal venous hypertension was assessed in the rat portal vein stenosis model. selective removal of pancreatic glucagon from the circulation was achieved by intravenous infusion of a highly specific glucagon antiserum. blood flow to splanchnic organs, kidneys, and testicles was measured with radioactive microspheres, and the reference-sample method. glucagon antiserum had no effect on blood flo ... | 1986 | 3777172 |
| participation of iron on the growth inhibition of pathogenic strains of mycobacterium avium and m. paratuberculosis in serum. | serum, which is low in freely-available iron, was bacteriostatic for m. avium and m. paratuberculosis irrespective of the ability of these organisms to produce the iron-chelators mycobactin and exochelin for iron acquisition. bacteriostasis was overcome when the serum was supplemented with exochelin or mycobactin, but only if the mycobacteria had previously adapted to growth in a low iron environment. the results imply that for studies on mycobacterial pathogenicity the test cultures should be g ... | 1986 | 3788345 |
| occurrence and distribution of halophilic vibrios in subtropical coastal waters of hong kong. | the summer occurrence and distribution of halophilic vibrios in the subtropical coastal waters of hong kong were investigated. the density of vibrios in six sample sites ranged from 90 to 6,700 per ml, which made up 0.41 to 40% of the total bacterial populations of these sample sites. the sucrose-positive vibrios were found to be much more common (88% of total vibrios) than the sucrose-negative ones. a total of 48 strains belonging to six vibrio species were fully characterized. among these, vib ... | 1986 | 3789725 |
| low-density lipoprotein apheresis and regression of atherosclerotic plaque in vitro. | short-term organ culture and primary cell culture of human aorta were used to study the effect of selective removal of low-density lipoproteins (ldls) from the surrounding medium (ldl apheresis) on the lipid content of cultured tissue and cells, respectively. ldl apheresis was performed by passing the culture medium through immunosorbent containing agarose-bound goat antibodies against human ldl. ldl apheresis promoted the decrease of lipids of all classes in the cultured atherosclerotic plaque. ... | 1986 | 3800702 |
| furosemide lowers intracranial pressure by inhibiting csf production. | furosemide administration effectively lowers intracranial pressure in newborn preterm and term rabbit pups. this effect may be due to the diuretic action of the drug, its ability to inhibit cerebrospinal fluid production or to a combination of both. to test these possibilities newborn rabbits were either injected with furosemide and left unmolested for 6 hours, or anaesthetized and subjected to ventriculocisternal perfusions. during the 6 hour postnatal period the decrease in body weight was 8 t ... | 1986 | 3811613 |
| lack of enzyme polymorphism in trypanosoma rangeli stocks from sylvatic and domiciliary transmission cycles in colombia. | although trypanosoma rangeli is biologically and morphologically distinct from trypanosoma cruzi, these two hemoflagellates are epidemiologically linked. we report the results of enzyme electrophoretic studies of t. rangeli stocks isolated from sylvatic and domiciliary rhodnius prolixus, and infected humans inhabiting foci in which t. cruzi was sympatrically transmitted. t. rangeli stocks displayed electrophoretically detectable polymorphism for only a single enzyme, isocitrate dehydrogenase (ic ... | 1987 | 3812885 |
| the primary structure of the hemoglobin of the european souslik (citellus citellus, rodentia). | the complete primary structures of two hemoglobin components of the european souslik (citellus citellus) are presented. the two hemoglobins have identical alpha-chains but differ in the amino-acid sequence of their beta-chains. the chain separation was achieved by chromatography on carboxymethyl-cellulose cm-52. amino-acid sequences were established by automatic liquid-phase and gas-phase edman degradation of the globin chains, of their tryptic peptides and of a peptide resulting from acidic hyd ... | 1986 | 3814354 |
| role of chitin in the survival of vibrio parahaemolyticus at different temperatures. | washed cells of vibrio parahaemolyticus declined in numbers when incubated in phosphate-buffered saline for 6 h at different temperatures. addition of chitin flakes to phosphate-buffered saline not only helped the organism to survive, but also resulted in an increase in cell numbers, particularly at 10 degrees c. the effect of chitin could not be simulated by n-acetylglucosamine, yeast extract, starch, or casein. | 1986 | 3815158 |
| evaluation of the hereditary syrian hamster cardiomyopathy by 31p nuclear magnetic resonance spectroscopy: improvement after acute verapamil therapy. | the relation between metabolic and functional derangement in various cardiomyopathies has not been well characterized. this information was specifically sought in a spontaneous cardiomyopathic model. metabolic and hemodynamic parameters were obtained in glucose-perfused beating hearts of 180-200-day-old cardiomyopathic syrian hamsters and age-matched healthy animals. this period in the cardiomyopathic hamster lifetime is intermediary between the necrotic phase and the appearance of heart failure ... | 1986 | 3815756 |
| [neuronal organization of conditioned inhibition]. | | 1986 | 3817182 |
| processing-independent in vitro translocation of cytochrome p-450(scc) precursor across mitochondrial membranes. | in the presence of a membrane-permeable metal chelator, bovine adrenal cortex mitochondria imported p-450(scc) precursor without processing of the amino-terminal extension peptide. the imported precursor was bound to the matrix side surface of the inner membrane. when the inhibition due to the metal chelator was removed, the imported precursor was processed to the mature form. unprocessed precursor was also detected in mitochondria when the import reaction was carried out at relatively low tempe ... | 1986 | 3818579 |
| similarity principles and intrinsic geometries: contrasting approaches to interspecies scaling. | we criticize standard allometric approaches on the grounds that they emphasize scaling to one variable at a time, whereas chemically reactive hydrodynamic systems involved in pharmacokinetic phenomena are of higher dimension. we show that attempts based on mechanical similitude to set a dosage that would be equivalent across species (for example, from mouse to humans) lead to ambiguous results. another failing of standard allometry may be its incapability to accommodate the neoteny of homo sapie ... | 1986 | 3820095 |
| defective biosynthesis of proteolipid protein in pelizaeus-merzbacher disease. | the brain of an 18-year-old patient with pelizaeus-merzbacher disease was examined by standard neuropathological and biochemical methods and by immunocytochemical and immunochemical techniques. analysis revealed a lack of myelin-specific lipids, but showed a residual immunoreactivity for myelin basic protein, myelin-associated glycoprotein, and 2',3'-cyclic nucleotide-3'-phosphodiesterase. examination by immunocytochemistry and enzyme-linked immunosorbent assay showed an absence of proteolipid a ... | 1987 | 3827224 |
| [an experimental study on adhesion blocking in simultaneous injury of tendon and nerve]. | the formation of adhesions between simultaneously injured tendon and nerve with and without adhesion blocking materials was studied by using a rabbit model. a collagen membrane, produced from adult bovine corium and with a negative electric charge induced by chemical modification, was used as the blocking material. microangiography and histological studies were performed to investigate the vascular supply to the adhesions. the following results were obtained. blood vessel proliferation was obser ... | 1985 | 3831164 |
| animal model for theophylline--cimetidine drug interaction. | cimetidine (300 mg i.v.) and theophylline (15 mg/kg i.v.) were studied in beagle dogs for possible drug interaction. the drugs were administered alone and in combination using a crossover design. although none of the determined pharmacokinetic parameters for theophylline changed significantly in the presence of cimetidine, a trend towards significance was found for the terminal half-life, area under the curve, and mean residence time. the magnitude in changes found in beagles is representative o ... | 1985 | 3831635 |
| a strain of urease-producing vibrio parahaemolyticus isolated in malaysia. | | 1985 | 3833915 |
| morphologic and histochemical studies of bone cells from sl-3 rats. | | 1985 | 3834472 |
| [effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. | | 1985 | 3835674 |
| plasma cell leukemia accompanied by polyclonal gammopathy and congenital tumor in the atrioventricular node. | a 32-year-old man with plasma cell leukemia and congenital tumor of the atrioventricular node is described. although m protein could not be detected by immunoelectrophoresis, the examination of bone marrow atypical plasma cells with immunofluorescent and immunoperoxidase showed that most of these plasma cells produced igg or iga. also some plasma cells possessed concomitantly both igg and iga. an electrocardiogram revealed complete atrioventricular block. the patient died of aspergillosis and bl ... | 1985 | 3837948 |
| degenerative lesions of a coronary chemoreceptor and nearby neural elements in the hearts of victims of sudden death. | a coronary chemoreceptor and its neighboring nerves and ganglia were studied by serial sections of tissue taken from the hearts of sixteen victims of sudden unexpected death. either focal or extensive inflammatory destruction was present in the chemoreceptors of 13 of the 16 hearts, and similar abnormalities of local nerves and ganglia were present in all 16 hearts. since an identical coronary chemoreceptor in the dog has previously been shown to be the site of origin of a powerful reflex with m ... | 1986 | 3837961 |
| isolation and characterization of neuropeptide y from porcine intestine. | the isolation and primary structure of intestinal neuropeptide y (npy) is described. the peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for npy. the amino acid sequence of this peptide is: tyr-pro-ser-lys-pro-asp-asn-pro-gly-glu-asp-ala-pro-ala-glu-asp-leu-ala- arg-tyr-tyr- ser-ala-leu-arg-his-tyr-ile-asn-leu-ile-thr-arg-gln-arg-tyr-nh2. this the structure of intestinal npy is identical to the npy of brain origin. | 1985 | 3838090 |
| theoretical mechanisms for synthesis of carcinogen-induced embryonic proteins: xiv. mutational and non-mutational mechanisms as subsets of a more general mechanism. part c. a defined cancer mutation. | the results of applying a mechanism of ethionine-induced embryonic gene derepressions to explain similar features found in hereditary tyrosinemia have been extended to a well defined cancer mutation. in all three cases, the described mechanism is compatible with the explanation for the etiology of embryonic like phenotypic expressions in cells and potentially for the carcinogenic process. the essence of the formulated process for a human bladder carcinoma mutation in the ras gene for a protein p ... | 1985 | 3853079 |
| generation of prostaglandin e2-like radioimmunoreactive material in human plasma during storage at -20 c but not at -80 c. | concentrations of prostaglandin e2 (pge2) as determined by radioimmunoassay following silicic-acid column chromatography in extracted samples (n = 5) of human plasma (na2edta: 18 mg/5 ml blood; indomethacin: 1mg/5 ml blood) were less than 1.0 pg/ml when the samples were extracted immediately after blood withdrawal. virtually identical concentrations of pge2 were determined in samples stored at -80 degrees c for up to 6 months. however, when the same analytical procedures were used after prolonge ... | 1985 | 3858888 |
| protein loss of bovine dental enamel during in-vitro subsurface demineralization. | a chemical system based on the dialysis principle was used to study protein loss of dental enamel during demineralization with an acetic-acid buffer solution containing calcium and phosphate, in which the fluoride-ion activity was kept constant. this resulted in a subsurface lesion, with a depth of about 130 microns. after demineralization, protein material was isolated from the demineralization solution. u.v. spectra of the protein showed strong absorbance between 240 and 300 nm. amino-acid com ... | 1985 | 3865639 |
| in vitro activity of ci-934, a quinolone carboxylic acid active against gram-positive and -negative bacteria. | ci-934 is a totally synthetic difluorinated quinolinecarboxylic acid with an ethyl-amino-methyl pyrrolidine side chain, which has broad-spectrum antibacterial activity, including particular potency directed against streptococci and staphylococci. the ci-934 mic (micrograms per milliliter) for 90% of the strains tested was 0.4 (range, 0.2 to 0.8) for a group of streptococci (pneumococci, viridans streptococci, streptococcus faecalis, and lancefield groups a, b, and c), 0.2 (0.05 to 0.2) for staph ... | 1985 | 3866513 |
| vibrio parahaemolyticus osteomyelitis of 12 years' duration. | | 1985 | 3868797 |
| gas chromatographic determination of mefloquine in human and dog plasma using electron-capture detection. | a sensitive and selective gas-liquid chromatographic method for the determination of plasma levels of mefloquine in human and dog plasma is described. the drug and internal standard were extracted from plasma at ph 9.0 into isopropyl acetate. after evaporation of the solvent, the residue was taken up in toluene and derivatised with heptafluorobutyrylimidazole. the derivative was quantified by gas-liquid chromatography on a 3% gc ge-se30 column with electron-capture detection. the limit of detect ... | 1985 | 3872308 |
| the effect of induced electric currents on bone after experimental osteotomy in sheep. | we have investigated the effect of currents induced by electromagnetic fields on the healing of the tibia of sheep after osteotomy, using objective and quantifiable criteria wherever possible. a battery-powered, induction apparatus was developed and was enclosed within the cast applied to the limb, so that the treated fractures received pulsed magnetic fields for 24 hours a day while the animals were freely mobile. in all, 13 sheep were treated and 13 were used as controls. the response was asse ... | 1985 | 3873459 |
| interleukin 1 released from beagle alveolar macrophages exposed to dust particles. | | 1985 | 3877837 |
| interleukin 1 released from beagle alveolar macrophages exposed to dust particles. | | 1985 | 3877837 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 4-1985. a 36-year-old man with a cardiac mass three years after renal transplantation. | | 1985 | 3880868 |
| proteoglycans in pathological conditions: atherosclerosis. | proteoglycans accumulate within the innermost layer (intima) of blood vessels during atherosclerosis. this accumulation is marked in some forms of human atherosclerosis and is particularly prominent in vessels that have been experimentally injured and have healed by the process of reendothelialization. the two major cell types of the arterial wall, endothelium and smooth muscle, are the major sources of arterial proteoglycans, and cell cultures have demonstrated that these cells synthesize at le ... | 1985 | 3881292 |
| characterization with monoclonal antibodies of a surface antigen of plasmodium falciparum merozoites. | the merozoite, the extracellular form of the erythrocyte stage of the malarial parasite, invades the erythrocyte and develops intracellularly. cloned hybridoma cell lines secreting monoclonal antibodies directed against the merozoite surface were selected by indirect immunofluorescent assay by using intact isolated merozoites. monoclonal antibodies to a 200,000 m.w. merozoite surface antigen were selected and were used to characterize this protein and its role in erythrocyte invasion. immunoelec ... | 1985 | 3881529 |
| cholera and other vibrioses in the united states. | | 1985 | 3881668 |
| [minimally transformed 3t3 cell lines as an object for research on the mechanisms of proliferation]. | the present review gives a detailed description of mouse cell lines 3t3 (swiss 3t3, balb/c 3t3, and nih 3t3) including their establishment, evolution, growth properties and formation of the specific monolayer with low saturation density. questionable views of the 3t3 cell origin are discussed. the main features of these continuous cell lines are shown, such as a high requirement for serum growth factors, substrate dependence, inhibition of cell division in dense monolayer and absence of oncogeni ... | 1985 | 3885522 |
| cloning and expression in escherichia coli of vibrio parahaemolyticus thermostable direct hemolysin and thermolabile hemolysin genes. | two hemolysin genes of vibrio parahaemolyticus wp1, a thermostable direct (tsd) hemolysin gene and a thermolabile hemolysin gene, were cloned into the pbr322 vector in escherichia coli k-12 c600. a large amount of the tsd hemolysin produced in e. coli k-12 accumulated in the periplasmic space. the tsd hemolysin gene was localized on a 0.9-kilobase hindiii-bamhi fragment by identifying qualitatively the production of the tsd hemolysin by a reverse passive hemagglutination assay in the osmotic sho ... | 1985 | 3886626 |
| shiga toxin--an expanding role in the pathogenesis of infectious diseases. | | 1985 | 3886803 |
| a mutagen precursor in chinese cabbage, indole-3-acetonitrile, which becomes mutagenic on nitrite treatment. | after treatment with nitrite, chinese cabbage showed direct-acting mutagenicity on salmonella typhimurium ta100 inducing 3100 revertants per g. one of the mutagen precursors that became mutagenic after nitrite treatment was isolated, and identified as indole-3-acetonitrile. after treatment with nitrite, 1 mg of indole-3-acetonitrile induced 17 400 revertants of ta100 and 21 000 revertants of ta98 without s9 mix. | 1985 | 3889600 |
| repair of dna single-strand breaks in x-irradiated yeast. ii. kinetics of repair as measured by the dna-unwinding method. | the kinetics of disappearance of single-strand breaks (ssb) from the dna of x-irradiated stationary yeast cells under liquid-holding conditions was found to proceed in a dose-independent manner up to a dose of at least 2400 gy, and was found to be complete after incubation of cells for 1 h. this was deduced from data for a yeast wild-type (wt) haploid and diploid strain as well as for rad52 haploid cells defective in dna double-strand break (dsb) repair. in all cases an initial fast repair compo ... | 1985 | 3889609 |
| spontaneous mutagenesis: the roles of dna repair, replication, and recombination. | there appears to be no dearth of mechanisms to explain spontaneous mutagenesis. in the case of base substitutions, data for bacteriophage t4 and especially for e. coli and s. cerevisiae suggest important roles in spontaneous mutagenesis for the error-prone repair of dna damage (to produce mutations) and for error-free repair of dna damage (to avoid mutagenesis). data from the very limited number of studies on the subject suggest that about 50% of the spontaneous base substitutions in e. coli, an ... | 1985 | 3889622 |
| cardioprotective effects of defibrotide in acute myocardial ischemia in the cat. | we investigated the effects of defibrotide (d), a natural polydeoxyribonucleotide, on acute myocardial ischemia (ami) in anesthetized cats. a permanent ligature was placed around the left anterior descending coronary artery (lad) 12-14 mm from its origin. ventricular fibrillation and death were exceptional and when they occurred the cats were not included in the evaluation. pretreatment of cats with d, 32 mg kg-1 h-1, i.v. infusion, maintained throughout the 5 h occlusion period, reduced ami-st ... | 1985 | 3890260 |
| cardioprotective effects of defibrotide in acute myocardial ischemia in the cat. | we investigated the effects of defibrotide (d), a natural polydeoxyribonucleotide, on acute myocardial ischemia (ami) in anesthetized cats. a permanent ligature was placed around the left anterior descending coronary artery (lad) 12-14 mm from its origin. ventricular fibrillation and death were exceptional and when they occurred the cats were not included in the evaluation. pretreatment of cats with d, 32 mg kg-1 h-1, i.v. infusion, maintained throughout the 5 h occlusion period, reduced ami-st ... | 1985 | 3890260 |
| a comparative study of enterotoxigenic escherichia coli, shigella, aeromonas, and vibrio as etiologies of diarrhea in northeastern thailand. | the incidence of enterotoxigenic escherichia coli (etec), shigella, aeromonas, and vibrio was determined in patients with diarrhea seen at a hospital in northeastern thailand, and compared with the incidence of these bacteria in household contacts and their neighbors. etec was identified in 17%, shigella in 9%, aeromonas in 9%, v. parahaemolyticus in 5%, and non-01 v. cholerae in 2% of 299 patients with diarrhea. these five species of bacteria were isolated more often from patients with diarrhea ... | 1985 | 3890578 |
| the microbiology of cooked prawns and shrimps on retail sale. | in an inter-laboratory survey, 148 samples of cooked prawns and shrimps were obtained at the point of sale to the consumer. salmonellae and vibrio parahaemolyticus were not detected. yersinia enterocolitica was isolated from three samples. results for total viable count and presence of escherichia coli and staphylococcus aureus complied well with available guidelines for imported cooked prawns, suggesting that the risk of food poisoning from retail samples of these foods in the south of england ... | 1985 | 3891850 |
| rapidly progressive glomerulonephritis accompanying legionnaires' disease. | a rapidly progressive, crescentic glomerulonephritis with acute oliguric renal failure occurred simultaneously with legionnaires' disease (ld) in a 52-year-old man. the diagnosis of ld was based on a sixfold rise in indirect fluorescent antibody titer against legionella pneumophila serogroup 4. treatment with erythromycin lactobionate resulted in a clinical resolution of pulmonary manifestations. the impairment of kidney function, however, was progressive and within two weeks led to end-stage re ... | 1985 | 3896188 |
| rapidly progressive glomerulonephritis accompanying legionnaires' disease. | a rapidly progressive, crescentic glomerulonephritis with acute oliguric renal failure occurred simultaneously with legionnaires' disease (ld) in a 52-year-old man. the diagnosis of ld was based on a sixfold rise in indirect fluorescent antibody titer against legionella pneumophila serogroup 4. treatment with erythromycin lactobionate resulted in a clinical resolution of pulmonary manifestations. the impairment of kidney function, however, was progressive and within two weeks led to end-stage re ... | 1985 | 3896188 |
| canine distemper virus does not infect oligodendrocytes in vitro. | dissociated canine brain cell cultures were infected with virulent canine distemper virus (cdv). double immunofluorescent labelling was done to simultaneously demonstrate viral antigen and specific glial cell markers. virus containing oligodendrocytes were not found at any stage of the infection. a certain proportion of the infected cells were shown to be astrocytes. it was concluded that cdv has no obvious tropism for oligodendrocytes which could explain the mechanism of demyelination in distem ... | 1985 | 3897461 |
| molecular assays for detection of ras oncogenes in human and animal tumors. | | 1985 | 3902219 |
| comparison of the modified elek test and wagatsuma agar for determination of the kanagawa phenomenon of vibrio parahaemolyticus. | the modified elek test and wagatsuma agar were compared for their ability to detect the kanagawa activity of 142 strains of vibrio parahaemolyticus. the performance of the modified elek test was on a par with that of the wagatsuma agar as far as positivity was concerned, and the test was far superior to wagatsuma agar in eliminating doubtful results. the results of the modified elek test were not unduly influenced by the different types of agar used. | 1985 | 3902884 |
| characterization of monoclonal antibodies against shiga-like toxin from escherichia coli. | three monoclonal antibodies, designated mab 16e6, mab 13c4, and mab 19g8, were produced which recognize shiga-like toxin (slt) from escherichia coli. all three monoclonal antibodies neutralized the cytotoxicity of e. coli slt and were able to immunoprecipitate intact labeled toxin with staphylococcus aureus protein a. the three antibodies were of the g1 heavy and kappa light chain classes. mab 16e6 bound to the b subunit of slt in western blots and also neutralized the lethality of the toxin for ... | 1985 | 3905611 |
| mechanism of sbcb-suppression of the recbc-deficiency in postreplication repair in uv-irradiated escherichia coli k-12. | the mechanism by which an sbcb mutation suppresses the deficiency in postreplication repair shown by recb recc mutants of escherichia coli was studied. the presence of an sbcb mutation in uvra recb recc cells increased their resistance to uv radiation. this enhanced resistance was not due to a suppression of the minor deficiency in the repair of dna daughter-strand gaps or to an inhibition of the production of dna double-strand breaks in uv-irradiated uvra recb recc cells; rather, the presence o ... | 1985 | 3911022 |
| specificity of surface molecules of adult brugia parasites: cross-reactivity with antibody from wuchereria, onchocerca and other human filarial infections. | the extent of structural and immunological similarity between surface antigens of three species of brugia filarial parasites was studied by lodogen-mediated surface labelling of adult worms of b. malayi, b. pahangi and b. timori. the close homology and cross-reactivity between these antigens reported in previous surface labelling studies with bolton-hunter reagent, was verified in this system. the surface antigens of adult b. pahangi are also recognised by antibody from patients with wuchereria ... | 1985 | 3911361 |
| a mucoid, sucrose-positive vibrio parahaemolyticus strain isolated from diarrhoeal stool. | | 1985 | 3912993 |
| human pancreatic growth hormone (gh)-releasing hormone stimulates gh synthesis and release in infant rats. an in vivo study. | administration of human pancreatic gh-releasing factor 1-40 (hpgrf-40) at doses of 1, 10, 20, 100, and 500 ng/100 g bw sc induced in 10-day-old rats a clear-cut rise in plasma gh 15-min post-injection, although the effect was not dose-related and peak gh levels were already present after the lowest grf dose. in 25-day-old rats, hpgrf induced only a slight rise in plasma gh at the dose of 500 ng/100 g bw sc, whereas it was completely ineffective at the lower doses. in 5-day-old rats, hpgrf (20 ng ... | 1985 | 3917905 |
| [in vitro drug-sensitivity test using human tumor clonogenic assay in lung cancer patients]. | it would be helpful for successful chemotherapy in cancer patients if a drug-sensitivity test in vitro could predict the exact response of an individual patient's tumor. we have investigated a drug-sensitivity test using human tumor clonogenic assay since 1980. in this paper, results obtained in lung cancer patients are discussed. specimens for testing were obtained from primary tumor, metastatic mass, malignant pleural and pericardial effusion, and affected bone marrow. drugs tested in this stu ... | 1985 | 3927847 |
| synthesis of dihomoprostaglandins from adrenic acid (7,10,13,16-docosatetraenoic acid) by human endothelial cells. | human umbilical vein endothelial cells were found to contain adrenic acid (22:4) in their cellular lipids. since this fatty acid may be metabolized by cyclooxygenase in the kidney, the metabolism of adrenic acid was studied in endothelial cell cultures. [14c]adrenic acid was metabolized to several more polar metabolites. two of these metabolites co-migrated on hplc with 1 alpha,1 beta-dihomo-8-ketoprostaglandin f1 alpha (the metabolite of 1 alpha, 1 beta-dihomoprostaglandin i2) and 1 alpha,1 bet ... | 1985 | 3931686 |
| the effects of glucose and an aldose reductase inhibitor on the sorbitol content and collagen synthesis of bovine retinal capillary pericytes in culture. | the absolute rate of collagen synthesis by cultured bovine retinal capillary pericytes, determined using the specific radioactivity of proline in the cellular amino acid pool, was compared in media containing different concentrations of glucose (5, 10 or 40 mm) and sorbinil (0.0 or 0.1 mm), an inhibitor of aldose reductase. the absolute rate of collagen synthesis, in proline molar terms, by pericytes in medium with 5 mm glucose was 3.3 +/- 1.9 (s.d.) pmol 10(-7) cells 24 hr-1, and increased sign ... | 1985 | 3933992 |
| modified taurocholate-tellurite-gelatin agar for improved differentiation of vibrio species. | a total of 78 strains, representing 21 vibrio species, were examined by using taurocholate-tellurite-gelatin agar (ttga) medium and modified ttga medium containing 4-methylumbelliferyl-beta-d-galactoside (150 micrograms/ml). modified ttga medium allowed for simple and direct detection of beta-d-galactosidase (beta-gal) activity. this feature, in conjunction with other differential characteristics of ttga medium, gave improved differentiation of the vibrios tested. the modified ttga medium allowe ... | 1985 | 3934207 |
| ghrf test in acromegalic patients for the evaluation of therapeutic results. | we studied here the clinical significance of ghrf test in evaluating therapeutic results in patients with acromegaly. three cases of acromegaly were studied at pre- and posttreatment. case 1 is a 42 year old woman, and her plasma gh showed slight increases responding to trh and arginine administration before therapy. eleven years after the pituitary irradiation, basal plasma gh decreased to low normal ranges and showed no response to the above two agents. only an insufficient increase in plasma ... | 1985 | 3937295 |
| fate of n-(2-hydroxypropyl)methacrylamide copolymers with pendent galactosamine residues after intravenous administration to rats. | n-(2-hydroxypropyl)methacrylamide (hpma) copolymers bearing galactosamine residues accumulate in the liver after intravenous administration to rats (duncan, r., kopecek, j., rejmanová, p. and lloyd, j.b. (1983) biochim. biophys. acta 755, 518-521). in this study hpma copolymers bearing pendent galactosamine residues (1.0-11.6 mol%) were injected intravenously into rats and their rates of blood clearance and liver accumulation were measured. a level of substitution of 4 mol% was found to be suffi ... | 1986 | 3942780 |
| distribution of gamma-carboxyglutamic acid residues in partially carboxylated human prothrombins. | the role of gamma-carboxyglutamic acid in prothrombin has been examined using partially carboxylated variant prothrombins isolated from a person with a hereditary defect in vitamin k-dependent carboxylation. these species differ in gamma-carboxyglutamic acid content, distribution, and function, as monitored by metal binding properties, conformational transitions, phospholipid binding, and calcium-dependent coagulant activity (borowski, m., furie, b. c., goldsmith, g. h., and furie, b. (1985) j. ... | 1986 | 3944102 |
| distribution of gamma-carboxyglutamic acid residues in partially carboxylated human prothrombins. | the role of gamma-carboxyglutamic acid in prothrombin has been examined using partially carboxylated variant prothrombins isolated from a person with a hereditary defect in vitamin k-dependent carboxylation. these species differ in gamma-carboxyglutamic acid content, distribution, and function, as monitored by metal binding properties, conformational transitions, phospholipid binding, and calcium-dependent coagulant activity (borowski, m., furie, b. c., goldsmith, g. h., and furie, b. (1985) j. ... | 1986 | 3944102 |
| purification and partial characterization of a non-o1 vibrio cholerae hemolysin that cross-reacts with thermostable direct hemolysin of vibrio parahaemolyticus. | a newly identified hemolysin (nag-rtdh), which is related to the thermostable direct hemolysin (vp-tdh) of vibrio parahaemolyticus produced by non-o1 vibrio cholerae, was studied. nag-rtdh was purified by successive column chromatographies on deae-cellulose and an immunoaffinity column coupled with anti-vp-tdh immunoglobulin. the molecular weight of nag-rtdh was estimated as 18,500, similar to that of vp-tdh, as judged by sodium dodecyl sulfate slab gel electrophoresis, but its charge or molecul ... | 1986 | 3957429 |
| interaction of histidine-rich glycoprotein with fibrinogen and fibrin. | histidine-rich glycoprotein (hrgp) is a human plasma and platelet protein of apparently diverse biological functions. in this study a new interaction for hrgp is described. hrgp specifically interacts with fibrinogen as demonstrated by two independent systems. using an enzyme-linked immunosorbent assay it was demonstrated that hrgp bound to adsorbed fibrinogen in a concentration-dependent and saturable manner, with an apparent dissociation constant (kd) of 6.7 nm. the binding was specific, rever ... | 1986 | 3958188 |
| influence of isoflurane on hypoxic pulmonary vasoconstriction in dogs. | the authors studied the influence of locally administered isoflurane anesthesia on the pulmonary vascular response to regional alveolar hypoxia (hypoxic pulmonary vasoconstriction [hpv]) over a range of cardiac outputs (cos) in seven mechanically ventilated, closed-chest dogs. the right lung was ventilated with 100% o2 throughout the study. the left lung was ventilated with either 100% o2 (normoxia) or an hypoxic gas mixture (hypoxia). different alveolar concentrations of isoflurane (0, 1, and 2 ... | 1986 | 3963450 |
| decontamination of cell lines by selective elimination of mycoplasmas. | | 1986 | 3964004 |
| decontamination of cell lines by selective elimination of mycoplasmas. | | 1986 | 3964004 |
| a one-year survey of campylobacter enteritis and other forms of bacterial diarrhoea in hong kong. | the following enteropathogens were isolated from the faeces of 769 (10.2%) of 7,545 patients of whom 5,704 had diarrhoea or abdominal pain, attending a teaching hospital in hong kong during one year: salmonellae 458 (6.1%); vibrio parahaemolyticus 125 (1.7%); campylobacters 108 (1.4%); shigellae 83 (1.1%); others 19 (0.3%). further identification of the campylobacter isolates showed that 63 (58%) were campylobacter jejuni biotype 1, 44 (41%) were c. coli and only one was c. jejuni biotype 2. sev ... | 1985 | 3973381 |
| seasonal distribution of vibrio parahaemolyticus in freshwater environs and in association with freshwater fishes in calcutta. | the seasonal distribution of vibrio parahaemolyticus in freshwater environs and in association with freshwater fishes was studied in 1982 and 1983. the occurrence of this organism in water and sediments at the three sites studied was very infrequent and was restricted to the summer months, although it was not always isolated during these months. the association of v. parahaemolyticus with plankton was chiefly confined to the summer months and progressively declined with the onset of monsoons, re ... | 1985 | 3977307 |
| metastatic nontraumatic clostridium septicum osteomyelitis. | nontraumatic clostridial infections are rare, but need to be diagnosed and treated early or high morbidity and mortality rates result. we believe that this is the first reported case of metastatic nontraumatic clostridium septicum osteomyelitis. early treatment with surgical debridement and parenteral antibiotics without hyperbaric oxygen was used. an associated occult rectal malignancy was discovered and treated. | 1985 | 3980945 |
| computer characterization of vibrio parahaemolyticus & allied sucrose-negative vibrios isolated from aquatic environs of porto novo. | | 1985 | 3988326 |
| nucleotide sequence of the thermostable direct hemolysin gene of vibrio parahaemolyticus. | the gene encoding the thermostable direct hemolysin of vibrio parahaemolyticus was characterized. this gene (designated tdh) was subcloned into pbr322 in escherichia coli, and the functional tdh gene was localized to a 1.3-kilobase hindiii fragment. this fragment was sequenced, and the structural gene was found to encode a mature protein of 165 amino acid residues. the mature protein sequence was preceded by a putative signal peptide sequence of 24 amino acids. a putative tdh promoter, determine ... | 1985 | 3988703 |
| [persistent chlamydia infection in cell culture]. | | 1985 | 3993192 |
| cadmium chloride susceptibility, a characteristic of campylobacter spp. | we report a simple diagnostic characteristic useful in the presumptive identification of campylobacter jejuni and campylobacter coli. filter paper disks impregnated with cadmium chloride were placed on streaked agar medium. zones of growth inhibition for campylobacter spp. occurred at 1.25 micrograms per disk. other enteropathogens (salmonella spp., shigella spp., vibrio cholerae, vibrio parahaemolyticus, escherichia coli, and yersinia enterocolitica) were resistant to at least 40 micrograms per ... | 1985 | 3998099 |
| effect of fecal pollution on vibrio parahaemolyticus densities in an estuarine environment. | vibrio parahaemolyticus densities in the narragansett bay, r.i., estuary were found to significantly correlate with the level of fecal pollution in the water. results of field investigations showed a definite association between the levels of this organism and those of escherichia coli, clostridium perfringens, and enterococci. densities of v. parahaemolyticus were greatest in the near-surface waters of contaminated areas and decreased sharply with both the distance from the sources of fecal pol ... | 1985 | 4004246 |
| isolation of a new drug-resistance plasmid from a strain of vibrio parahaemolyticus. | a new r plasmid, psa55, with a molecular weight of 112 megadaltons (md), was isolated from a strain of vibrio parahaemolyticus with multiple drug resistance. the psa55 plasmid conferred on its host resistance to chloramphenicol, tetracycline, streptomycin, kanamycin, ampicillin, trimethoprim and 2,4-diamino-6,7-diisopropyl pteridine, and belongs to incompatibility group c. the plasmid was transferable to escherichia coli, v. parahaemolyticus, v. alginolyticus and nag bivrio at a frequency of 10( ... | 1985 | 4010538 |
| biliary excretion and enterohepatic circulation of 1-nitropyrene metabolites in fischer-344 rats. | 1-nitropyrene (1-np), present in diesel engine emissions, is a potent mutagen to bacteria, such as those found in mammalian intestinal tract, which contain nitroreductase enzymes. the purposes of this study were to determine the importance of bile as a route of excretion of 1-np metabolites and to determine if reabsorption of biliary metabolites required the presence of intestinal bacteria. the bile ducts of male fischer-344 rats were cannulated, 0.3 or 1.2 mumoles [3h]1-np was given i.v., and b ... | 1985 | 4015679 |
| altered membrane association of glycogen phosphorylase in the dystrophic chicken. | the subcellular distribution of glycogen phosphorylase in pectoralis muscle from normal and dystrophic chickens was determined as a function of age. a substantially larger proportion of the total activity was associated with membranous cellular organelles, both mitochondria and sarcoplasmic reticulum, in preparations from dystrophic birds. the difference could be detected as early as 2 weeks ex ovo. interaction of phosphorylase with cellular membranes may provide a probe for the underlying membr ... | 1985 | 4016151 |
| the instability of intestinal clostridial colonisation in living beings. | | 1985 | 4022979 |
| reentrant ventricular arrhythmias in the late myocardial infarction period in the dog. 13. correlation of activation and refractory maps. | isochronal maps of ventricular activation were analyzed in dogs 3-5 days after ligation of the left anterior descending coronary artery utilizing a 64-channel multiplexer. isochronal maps of the effective refractory period were determined from 62 epicardial sites and correlated with the activation maps. the ischemia occurring in the surviving epicardial layer prolonged refractoriness in a spatially nonuniform manner. the resulting pattern of refractoriness on the epicardial surface resembled con ... | 1985 | 4028346 |
| detection of the thermostable direct hemolysin gene and related dna sequences in vibrio parahaemolyticus and other vibrio species by the dna colony hybridization test. | a specific gene probe for the vibrio parahaemolyticus thermostable direct hemolysin gene was constructed and used to examine the presence or absence of the thermostable direct hemolysin gene or related dna sequences in v. parahaemolyticus and other vibrios by the dna colony hybridization method. the gene probe consisted of a 406-base-pair, completely internal fragment covering 71% of the structural gene with psti linkers added to the ends. six copies of this 415-base-pair psti fragment were clon ... | 1985 | 4030087 |
| arsenic toxicosis and suspected chromium toxicosis in a herd of cattle. | arsenic toxicosis and suspected chromium toxicosis were diagnosed in a herd of cattle that ingested ashes from lumber treated with copper, chromium, and arsenic. findings included peracute death, depression, ataxia, weakness, recumbency, and watery diarrhea. chemical analyses of liver, kidney, abomasal contents, rumen contents, and ashes revealed high concentrations of arsenic and chromium. histologically, specimens of abomasum and duodenum had diffuse mucosal degeneration and engorged capillari ... | 1985 | 4030455 |
| the incidence of rhodococcus (corynebacterium) equi in domestic animals and soil. | | 1985 | 4032938 |
| the role of adenosine in the regulation of coronary blood flow in newborn lambs. | adenosine is a metabolic vasodilator of the coronary vessels in the adult. whether it plays a similar role in the regulation of coronary blood flow (cbf) in the newborn is not known. we evaluated changes in adenosine release during periods of decreased oxygen supply (hypoxia) and increased oxygen demand (dobutamine infusions). in anesthetized open-chest lambs (age 1 to 8 days), aortic and coronary sinus adenosine concentrations, circumflex cbf, and myocardial oxygen consumption (mvo2) were measu ... | 1985 | 4035574 |
| inhibition of lipases by proteins. a kinetic study with dicaprin monolayers. | we report further investigations on protein inhibition of pancreatic and microbial lipases carried out with the monolayer technique. when beta-lactoglobulin a, melittin, serum albumin, myoglobin, and a protein inhibiting lipase from soybean were preincubated with a dicaprin film at a surface pressure of 35 dynes/cm, no activity was detected with horse pancreatic or rhizopus delemar lipases. by contrast, rhizopus arrhizus and geotrichum candidum lipase activities were not impaired under the same ... | 1985 | 4038705 |
| local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males. | the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ... | 1985 | 4040921 |
| enzyme-linked immunosorbent assays for detection of thermostable direct hemolysin of vibrio parahaemolyticus. | several systems for enzyme-linked immunosorbent assay (elisa) of thermostable direct hemolysin (tdh) of vibrio parahaemolyticus were tested, and single-antibody sandwich elisa systems gave satisfactory results. elisa was able to detect as little as several nanograms of purified tdh per milliliter. the method of de jong (j. clin. microbiol. 17:928-930, 1983) and the glutaraldehyde method were successful for preparing conjugates of alkaline phosphatase and anti-tdh antibody. tdh in fluids in intes ... | 1985 | 4044797 |
| efficacy of trazodone in narcolepsy. | a 25-year-old man with narcolepsy and cataplexy experienced partial relief of symptomatology following administration of methylphenidate. moreover, the latter caused extreme agitation and aggression. however, administration of the antidepressant agent trazodone resulted in almost complete alleviation of the narcoleptic and cataplectic attacks within 48 h after initiation of therapy. trazodone, a novel antidepressant agent may be useful in the long-term management of individuals suffering from na ... | 1985 | 4054183 |