| A platelet-mediated system for shuttling blood-borne bacteria to CD8a+ dendritic cells depends on glycoprotein GPIb and complement C3. | The acquisition of pathogen-derived antigen by dendritic cells (DCs) is a key event in the generation of cytotoxic CD8(+) T cell responses. In mice, the intracellular bacterium Listeria monocytogenes is directed from the blood to splenic CD8a(+) DCs. We report that L. monocytogenes rapidly associated with platelets in the bloodstream in a manner dependent on GPIb and complement C3. Platelet association targeted a small but immunologically important portion of L. monocytogenes to splenic CD8a(+) ... | 2011 | 22037602 |
| the effect of nisin on l. monocytogenes in turkish fermented sausages (sucuks). | turkish fermented sausage (sucuk) is a traditional, well-known meat product in turkey. the aim of this study was to evaluate the effect of different nisin concentrations on listeria monocytogenes in experimentally contaminated sucuk. analyses were performed on at 0, 1, 3, 5, 7, 10, 15, 20, 25 and 30days for l. monocytogenes and other microbiological parameters (total mesophilic aerobic bacteria and lactic acid bacteria) and physico-chemical parameters (ph, a(w) and moisture content).the sucuk do ... | 2007 | 22064303 |
| characterisation of naturally fermented sausages produced in the north east of italy. | in the friuli venezia giulia region, in the north east of italy, a traditional fermented sausage is produced without the use of microbial starters. it is characterized at the end of the ripening period by accentuated acidity, slight sourness and elastic, semi-hard consistency. in this study, three fermentations, carried out in different seasons (winter, spring and summer) were followed analyzing the microbiological, physicochemical and sensory aspects of this product. the sausages were character ... | 2005 | 22062975 |
| Cold Plasma Decontamination of Foods. | Cold plasma is a novel nonthermal food processing technology that uses energetic, reactive gases to inactivate contaminating microbes on meats, poultry, fruits, and vegetables. This flexible sanitizing method uses electricity and a carrier gas, such as air, oxygen, nitrogen, or helium; antimicrobial chemical agents are not required. The primary modes of action are due to UV light and reactive chemical products of the cold plasma ionization process. A wide array of cold plasma systems that operat ... | 2011 | 22149075 |
| Effects of dietary yeast extract on turkey stress response and heterophil oxidative burst activity. | 1. Effective nutritional approaches to counteract the negative effects of stress may provide food animal producers with useful alternatives to antibiotics. In this study, turkeys were fed on a standard diet, or the same diet supplemented with yeast extract (YE), to determine if YE would improve disease resistance in a stress model. 2. At 16 weeks of age, half of the birds were exposed to a bacterial challenge using a coarse spray of the pen environment. A subset of control and challenged birds w ... | 2011 | 21919572 |
| Discovery of novel biopreservation agents with inhibitory effects on growth of food-borne pathogens and their application to seafood products. | Selection of protective cultures is relevant in order to biopreserve and improve the functional safety of food products, mainly through inhibition of spoilage and/or pathogenic bacteria. Accordingly, the present study investigated potential applications of lactic acid bacteria (LAB) in the biopreservation of fish and shellfish products. For this purpose, a collection of 84 LAB strains isolated from sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) was identified and characterized for ... | 2011 | 22041547 |
| effect of nisin and its combination with sodium chloride on the survival of listeria monocytogenes added to raw buffalo meat mince. | antilisterial activity of nisin (nisaplin), alone at concentrations of 400 and 800 iu/g and in combination with 2% sodium chloride was incorporated in raw buffalo meat mince. samples of the raw meat mince were inoculated with 10(3) colony forming units (cfu)/g of l. monocytogenes and stored at 4°c for 16 days and at 37°c for 36 h. initial estimates of ph, extract release volume, mesophilic and psychrophilic counts were found to be 5.74, 48 ml, 3.5×10(5) and 1.0×10(5) cfu/g of meat, respectively. ... | 2000 | 22062071 |
| Small molecule modulators of Listeria monocytogenes biofilm development. | Listeria monocytogenes is an important food-borne pathogen whose ability to form disinfectant tolerant biofilms on a variety of surfaces presents a food safety challenge for manufacturers of ready-to-eat products. Here we developed a high-throughput biofilm assay for L. monocytogenes and as a proof of principle, used it to screen a 80-compound protein kinase inhibitor library to identify molecules that perturb biofilm development. The screen yielded molecules toxic to multiple strains of Listeri ... | 2011 | 22194285 |
| Antibacterial activity of different degree of hydrolysis of palm kernel expeller peptides against spore-forming and non-spore-forming bacteria. | The goal of this study was to determine inhibitory effect of palm kernel expeller (PKE) peptides of different degree of hydrolysis (DH %) against spore-forming bacteria Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus stearothermophillus, Bacillus subtilis, Bacillus thuringiensis, Clostridium perfringens; and non-spore-forming bacteria Escherichia coli, Lisinibacillus sphaericus, Listeria monocytogenes, Pseudomonas a ... | 2011 | 21848644 |
| TLR2 and RIP2 Pathways Mediate Autophagy of Listeria monocytogenes via Extracellular Signal-regulated Kinase (ERK) Activation. | Listeria monocytogenes is a facultative intracellular pathogen that invades both phagocytic and non-phagocytic cells. Recent studies have shown that L. monocytogenes infection activates the autophagy pathway. However, the innate immune receptors involved and the downstream signaling pathways remain unknown. Here, we show that macrophages deficient in the TLR2 and NOD/RIP2 pathway display defective autophagy induction in response to L. monocytogenes. Inefficient autophagy in Tlr2(-/-) and Nod2(-/ ... | 2011 | 22033934 |
| Vibralactone as a tool to study the activity and structure of the ClpP1P2 complex from Listeria monocytogenes. | | 2011 | 21954175 |
| Intracellular bacteria and adverse pregnancy outcomes. | This review considers the role of intracellular bacteria in adverse pregnancy outcomes, such as miscarriage, stillbirths, and preterm labour. The cause of miscarriage, stillbirth and preterm labour often remains unexplained. Intracellular bacteria that grow either poorly or not at all on media used routinely to detect human pathogens could be the aetiological agents of these obstetric conditions. For example, Listeria monocytogenes and Coxiella burnetti are intracellular bacteria that have a pre ... | 2011 | 21884294 |
| Changes in gene expression during adaptation of Listeria monocytogenes to the soil environment. | Listeria monocytogenes is a ubiquitous opportunistic pathogen responsible for listeriosis. In order to study the processes underlying its ability to adapt to the soil environment, whole-genome arrays were used to analyse transcriptome modifications 15 minutes, 30 minutes and 18 h after inoculation of L. monocytogenes EGD-e in soil extracts. Growth was observed within the first day of incubation and large numbers were still detected in soil extract and soil microcosms one year after the start of ... | 2011 | 21966375 |
| Quality Parameters and Antioxidant and Antibacterial Properties of Some Mexican Honeys. | A total of 14 Mexican honeys were screened for quality parameters including color, moisture, proline, and acidity. Antioxidant properties of complete honey and its methanolic extracts were evaluated by the DPPH, ABTS, and FRAP assays. In addition, the antimicrobial activity of complete honeys against Bacillus cereus ATCC 10876, Listeria monocytogenes Scott A, Salmonella Typhimurium ATCC 14028, and Sthapylococcus aureus ATCC 6538 was determined. Most of honeys analyzed showed values within qual ... | 2011 | 22133067 |
| Evaluation of applied biosystems MicroSeq real-time PCR system for detection of Listeria monocytogenes in food. Performance Tested Method 011002. | Increasingly, more food companies are relying on molecular methods, such as PCR, for pathogen detection due to their improved simplicity, sensitivity, and rapid time to results. This report describes the validation of a new Real-Time PCR method to detect Listeria monocytogenes in nine different food matrixes. The complete system consists of the MicroSEQ L. monocytogenes Detection Kit, sample preparation, the Applied Biosystems 7500 Fast Real-Time PCR instrument, and RapidFinder Express software. ... | 2011 | 22165012 |
| [Bacteriological quality of traditional, organic and hydroponic cultured lettuce in Costa Rica]. | The main objective of this work was to evaluate the microbiological quality of lettuces commercialized in the Metropolitan Area of San José, Costa Rica, and cultured in different ways, in order to detect differences between the culturing methods and the risk that these products may represent for Public Health. The study was done at the Food Microbiology Laboratory, Universidad de Costa Rica, from March to July, 2010. 30 lettuce samples were analyzed (10 obtained by traditional culture, 10 by org ... | 2011 | 22097292 |
| control of listeria monocytogenes growth in a ready-to-eat poultry product using a bacteriophage. | a bacteriophage (phage) that infected strains of the species listeria monocytogenes as well as listeria ivanovii and listeria welshimeri, but not listeria grayi or listeria innocua, was isolated from sheep faeces. the phage had a contractile tail and an icosohedral head indicating that it was a myovirus, and was morphologically similar to phage a511. at 30 °c, phages added at 5.2 × 10⁷ pfu ml⁻¹ prevented the growth in broth of l. monocytogenes present at approximately twice this concentration fo ... | 2011 | 21925027 |
| influence of nisin and selected meat additives on the antimicrobial effect of ovotransferrin against listeria monocytogenes. | the objective of this study was to determine the effect of nisin and selected meat additives (salt, lactate, lactate-diacetate combination, and polyphosphate) on the antimicrobial activities of ovotransferrin (otf) against the growth of listeria monocytogenes. a bioscreen c turbidometer (oy growth curves ab ltd., helsinki, finland) was used to evaluate the effect of various concentrations of nisin and individual meat additives on the antilisterial activity of otf in brain heart infusion (bhi) br ... | 2011 | 22010245 |
| caveolin-1 orchestrates tcr synaptic polarity, signal specificity, and function in cd8 t cells. | tcr engagement triggers the polarized recruitment of membrane, actin, and transducer assemblies within the t cell-apc contact that amplify and specify signaling cascades and t effector activity. we report that caveolin-1, a scaffold that regulates polarity and signaling in nonlymphoid cells, is required for optimal tcr-induced actin polymerization, synaptic membrane raft polarity, and function in cd8, but not cd4, t cells. in cd8(+) t cells, caveolin-1 ablation selectively impaired tcr-induced n ... | 2011 | 21849673 |
| rapid, sensitive, and simultaneous detection of three foodborne pathogens using magnetic nanobead-based immunoseparation and quantum dot-based multiplex immunoassay. | losses caused by foodborne diseases are enormous in terms of human life, illness, medical costs, and food product recalls. rapid detection of multiple bacterial pathogens in foods is extremely important to ensure food safety. the objective of this research was to develop a multiplex immunoassay by integrating magnetic nanobeads (mnbs) for immunoseparation with quantum dots (qds) as fluorescent labels for rapid, sensitive, and simultaneous detection of three major pathogenic bacteria, salmonella ... | 2011 | 22186043 |
| spatial competition with lactococcus lactis in mixed-species continuous-flow biofilms inhibits listeria monocytogenes growth. | surfaces in industrial settings provide a home for resident biofilms that are likely to interact with the attachment, growth and survival of pathogens such as listeria monocytogenes. experimental results have indicated that l. monocytogenes cells were inhibited by the presence of a model resident flora (lactococcus lactis) in dual-species continuous flow-biofilms, and are spatially restricted to the lower biofilm layers. using a new, simplified individual-based model (ibm) that simulates bacteri ... | 2011 | 22043862 |
| antilisterial activity of lactic acid bacteria isolated from "alheiras" (traditional portuguese fermented sausages): in situ assays. | a total of 226 lactic acid bacteria (lab) isolated from "alheira", a traditional portuguese fermented sausage, were screened for antagonistic activity against some pathogenic microorganisms, including listeria monocytogenes. the objective was to isolate lab with antibacterial activity from "alheiras" and to select strains that could be used in "alheira" production. isolates displaying antibacterial activity against listeria innocua and l. monocytogenes were investigated for the nature of the ant ... | 2007 | 22061260 |
| comparison of polymerase chain reaction and conventional methods for diagnosis of listeria monocytogenes isolated from different clinical specimens and food stuffs. | listeria monocytogenes has emerged as a significant food borne pathogen in recent decades. polymerase chain reaction (pcr) is deemed to be more reliable than conventional methods of identification. this work aimed to evaluate the accuracy of pcr in comparison to conventional methods for the diagnosis of l. monocytegenes from different clinical specimens and food stuffs. | 2011 | 22239022 |
| bicyclo[4.4.0]decane oxygenated sesquiterpenes from eryngium maritimum essential oil. | investigation of the essential oil of the aerial parts of eryngium maritimum l. from corsica led to the isolation of one known sesquiterpene ( 1) and three new oxygenated sesquiterpenes with a muurolane or cadinane skeleton ( 2- 4). structure assignments of 4 βh -muurol-9-en-15-al ( 1), 4 βh -cadin-9-en-15-al ( 2), 4 βh -muurol-9-en-15-ol ( 3) and 4 βh -cadin-9-en-15-ol ( 4) were carried out by gc/ms (ei and ci) and comprehensive 1d and 2d nmr spectroscopy. antibacterial activity of eryngium m ... | 2012 | 22258696 |
| characterization of functional, safety, and probiotic properties of enterococcus faecalis ugra10, a new as-48-producer strain. | enterococcus faecalis ugra10, a new as-48-producer strain, has been isolated from a spanish sheep's cheese. the inhibitory substance produced by e. faecalis ugra10 was purified and characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry, confirming its identity with as-48 enterocin (7.150 da). subsequent genetic analysis showed the existence of the as-48 gene cluster on a plasmid of approximately 70-kb. the ugra10 strain was examined for safety properties ... | 2012 | 22265284 |
| behaviour of listeria monocytogenes isolates through gastro-intestinal tract passage simulation, before and after two sub-lethal stresses. | the effects of previous exposure to sub-lethal acidic and osmotic stresses on the survival of listeria monocytogenes during exposure to gastro-intestinal (gi) tract simulation, was investigated. six l. monocytogenes strains isolated from cheeses were selected and exposed to high salt concentrations or acidic conditions and their viability compared in quick and slow digestions. the results demonstrated that (i) all isolates were more sensitive to the exposure to acidic than to osmotic sub-lethal ... | 2012 | 22265279 |
| p47(phox) directs murine macrophage cell fate decisions. | macrophage differentiation and function are pivotal for cell survival from infection and involve the processing of microenvironmental signals that determine macrophage cell fate decisions to establish appropriate inflammatory balance. nadph oxidase 2 (nox2)-deficient chronic granulomatous disease (cgd) mice that lack the gp91(phox) (gp91(phox-/-)) catalytic subunit show high mortality rates compared with wild-type mice when challenged by infection with listeria monocytogenes (lm), whereas p47(ph ... | 2012 | 22222227 |
| effects of slightly acidic low concentration electrolyzed water on microbiological, physicochemical, and sensory quality of fresh chicken breast meat. | anticmicrobial effect of slightly acidic low concentration electrolyzed water (slalcew) and strong acidic electrolyzed water (staew) on fresh chicken breast meat was evaluated in this study. meat samples each of 10 ± 0.2 g in weight and 2.5 × 2.5 cm(2) in size were experimentally inoculated with listeria monocytogenes (atcc 19115) and salmonella typhimurium (atcc 14028) and subjected to dipping treatment (22 ± 2 °c for 10 min) with slalcew and staew. shelf-life study was conducted for inoculate ... | 2012 | 22260115 |
| resistance of pathogenic bacteria on the surface of stainless steel depending on attachment form and efficacy of chemical sanitizers. | various bacteria including food spoilage bacteria and pathogens can form biofilms on different food processing surfaces, leading to potential food contamination or spoilage. therefore, the survival of foodborne pathogens (escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium, staphylococcus aureus, cronobacter sakazakii) in different forms (adhered cells, biofilm producing in tsb, biofilm producing at rh 100%) on the surface of stainless steel and stored at various relative hu ... | 2012 | 22225983 |
| growth inhibition of foodborne pathogens by oenococcus oeni. | to explore the possibility of using oenococcus oeni to inhibit foodborne pathogens, and to characterize antimicrobial compounds produced by o. oeni, 24 strains of o. oeni were tested for their ability to inhibit growth of foodborne pathogens, escherichia coli o157:h7, salmonella enteritidis, and listeria monocytogenes by using the spot-on-lawn method. of the 24 strains, 17 strains were able to inhibit all 3 pathogens in this study. proteases, catalase, and buffer solutions were used for determin ... | 2012 | 22260113 |
| virulence factors that modulate the cell biology of listeria infection and the host response. | the gram-positive bacterial pathogen listeria monocytogenes has become one of the best studied models in infection biology. this review will update our knowledge of listeria virulence factors and highlight their role during the listeria infection process. | 2012 | 22244577 |
| synergies of carvacrol and 1,8-cineole to inhibit bacteria associated with minimally processed vegetables. | this study assessed the occurrence of an enhancing inhibitory effect of the combined application of carvacrol and 1,8-cineole against bacteria associated with minimally processed vegetables using the determination of fractional inhibitory concentration (fic) index, time-kill assay in vegetable broth and application in vegetable matrices. their effects, individually and in combination, on the sensory characteristics of the vegetables were also determined. carvacrol and 1,8-cineole displayed minim ... | 2011 | 22273461 |
| in vitro and in situ antimicrobial action and mechanism of glycinin and its basic subunit. | glycinin, basic subunit and β-conglycinin were isolated from soybean protein isolate and tested for their antimicrobial action against pathogenic and spoilage bacteria as compared to penicillin. the three fractions exhibited antibacterial activities equivalent to or higher than penicillin in the next order; basic subunit>glycinin>β-conglycinin with mic of 50, 100 and 1000μg/ml, respectively. the ic(50%) values of the basic subunit, glycinin and β-conglycinin against listeria\monocytogenes were 1 ... | 2011 | 22236762 |
| innate immune pathways triggered by listeria monocytogenes and their role in the induction of cell-mediated immunity. | acquired cell-mediated immunity to listeria monocytogenes is induced by infection with live, replicating bacteria that grow in the host cell cytosol, whereas killed bacteria, or those trapped in a phagosome, fail to induce protective immunity. in this chapter, we focus on how l. monocytogenes is sensed by the innate immune system, with the presumption that innate immunity affects the development of acquired immunity. infection by l. monocytogenes induces three innate immune pathways: an myd88-de ... | 2012 | 22244582 |
| the intestinal secreted c-type lectin reg3b attenuates salmonellosis but not listeriosis in mice. | background: the reg3 protein family, including the human member designated pancreatitis-associated protein, are secreted proteins that contain a c-type lectin domain involved in carbohydrate binding. they are expressed by intestinal epithelial cells. colonization of germ-free mice and intestinal infection with pathogens increases the expression of reg3g and reg3b in the murine ileum. reg3g is directly bactericidal for gram-positive bacteria but the exact role of reg3b in bacterial infections is ... | 2012 | 22252863 |
| chemical compositions of the essential oils of aerial parts of chamaemelum mixtum (l.) alloni. | the chemical compositions of the aerial parts essential oils of chamaemelum mixtum (l.) alloni from corsica and sardinia were investigated employing gas chromatography and gas chromatography/mass spectrometry. the structure of (z)-heptadeca-9,16-dien-7-one, a natural compound not previously described, was elucidated by gas chromatography/mass spectrometry (electron impact and chemical ionization) and one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy. the variation in ch ... | 2012 | 22225420 |
| a novel restriction- modification system is responsible for temperature- dependent phage resistance in listeria monocytogenes ecii. | listeria monocytogenes epidemic clone ii (ecii) strains are unusual in being completely resistant to phage when grown at low temperatures (≤30°c). in the current study we constructed and characterized a mariner-based mutant (j46c) of the ecii strain h7550-cd(s) that lacked temperature-dependent resistance to phage. the transposon was localized in lmoh7858_2753 (orf 2753), a member of a 12-orf genomic island unique to ecii strains. orf 2753 and orf 2754 exhibited homologies to restriction endonuc ... | 2012 | 22247158 |
| choosing orientation: influence of cargo geometry and acta polarization on actin comet tails. | networks of polymerizing actin filaments can propel intracellular pathogens and drive movement of artificial particles in reconstituted systems. while biochemical mechanisms activating actin network assembly have been well characterized, it remains unclear how particle geometry and large-scale force balance affect emergent properties of movement. we reconstituted actin-based motility using ellipsoidal beads resembling the geometry of listeria monocytogenes. beads coated uniformly with the l. mon ... | 2012 | 22219381 |
| bacteriological quality of raw milk used for production of a brazilian farmstead raw milk cheese. | abstract the objective of this study was to evaluate the bacteriological quality of raw cow's milk utilized for the production of traditional minas serro cheese, a brazilian farmstead raw milk cheese. raw milk samples were collected from six farmstead cheese operations manufacturing raw milk cheese from cow's milk. coliform count (cc) and escherichia coli counts were determined using petrifilm(™) ec plates, and staphylococcus aureus counts were determined using petrifilm(™) staph express count ... | 2012 | 22217108 |
| inflammatory monocytes and neutrophils are licensed to kill during memory responses in vivo. | immunological memory is a hallmark of b and t lymphocytes that have undergone a previous encounter with a given antigen. it is assumed that memory cells mediate better protection of the host upon re-infection because of improved effector functions such as antibody production, cytotoxic activity and cytokine secretion. in contrast to cells of the adaptive immune system, innate immune cells are believed to exhibit a comparable functional effector response each time the same pathogen is encountered ... | 2011 | 22241983 |
| listeria monocytogenes and its products as agents for cancer immunotherapy. | this review covers the use of listeria monocytogenes and its virulence factors as cancer immunotherapeutics. we describe their development as vectors to carry protein tumor antigen and eukaryotic dna plasmids to antigen-presenting cells and efforts to harness their tumor-homing properties. we also describe their use as vectors of angiogenic molecules to induce an immune response that will destroy tumor vasculature. the background knowledge necessary to understand the biology behind the rationale ... | 2012 | 22244580 |
| survival and growth of foodborne pathogens in minimally processed vegetables at 4 and 15 °c. | we conducted this study to investigate the survival and growth of pathogens on fresh vegetables stored at 4 and 15 °c. vegetables (romaine lettuce, iceberg lettuce, perilla leaves, and sprouts) were inoculated with 4 pathogens (salmonella enterica serovar typhimurium, staphylococcus aureus, listeria monocytogenes, and escherichia coli o157:h7) and stored at 2 different temperatures for different periods of time (3, 6, 9, 12, and 15 d at 4 °c and 1, 2, 3, 5, and 7 d at 15 °c). populations of the ... | 2012 | 22260117 |
| fsl j1-208: a virulent uncommon phylogenetic lineage iv listeria monocytogenes strain with a small chromosome size and a putative virulence plasmid carrying internalin-like genes. | the bacterial genus listeria contains both saprotrophic and facultative pathogenic species. a small genome size has been suggested to be associated with the loss of pathogenic potential of l. welshimeri and l. seeligeri. in this paper we present data on the genome of l. monocytogenes strain fsl j1-208, a representative of phylogenetic lineage iv. although this strain was isolated from a clinical case in a caprine host and has no decreased invasiveness in human intestinal epithelial cells, our an ... | 2012 | 22247147 |
| recombinant probiotic expressing listeria adhesion protein attenuates listeria monocytogenes virulence in vitro. | listeria monocytogenes, an intracellular foodborne pathogen, infects immunocompromised hosts. the primary route of transmission is through contaminated food. in the gastrointestinal tract, it traverses the epithelial barrier through intracellular or paracellular routes. strategies to prevent l. monocytogenes entry can potentially minimize infection in high-risk populations. listeria adhesion protein (lap) aids l. monocytogenes in crossing epithelial barriers via the paracellular route. the use o ... | 2012 | 22235279 |
| culture confirmation of listeria monocytogenes using tmrna as a diagnostics target. | | 2012 | 22261139 |
| incidence of salmonella, listeria monocytogenes, escherichia coli o157:h7, and staphylococcal enterotoxin in two types of mexican fresh cheeses. | handcrafted fresh cheeses are popular among consumers in mexico. however, unsafe raw materials and inadequate food safety practices during cheese manufacture and preservation make them a potential public health risk. the incidence of salmonella, listeria, escherichia coli o157:h7, and staphylococcal enterotoxin was analyzed in two types of fresh cheese (panela and adobera) commonly marketed in mexico. a total of 200 samples, 100 panela and 100 adobera, were acquired from 100 wholesale milk prod ... | 2012 | 22221358 |
| monocyte-mediated immune defense against murine listeria monocytogenes infection. | infection of mice with listeria monocytogenes induces a robust innate inflammatory response that restricts bacterial growth in the liver and spleen prior to the development of protective t cell responses. ly6c(hi) monocytes contribute to the innate immune response following l. monocytogenes infection and in their absence, mice rapidly succumb to infection. emigration of ly6c(hi) monocytes from the bone marrow into the circulation is the first step in their recruitment to sites of l. monocytogene ... | 2012 | 22244581 |
| the antimicrobial properties of the lowbush blueberry (vaccinium angustifolium) fractional components against foodborne pathogens and the conservation of probiotic lactobacillus rhamnosus. | the antimicrobial properties of lowbush blueberry (vaccinium angustifolium) were studied against escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium, and lactobacillus rhamnosus to determine which fractional components have antimicrobial effects and which microorganisms are most susceptible to these antimicrobial properties. lowbush blueberry extract (f1) was separated using a c-18 sep-pak cartridge into monomeric phenolics (f2) and anthocyanins plus proanthocyanidins (f3). ... | 2012 | 22265292 |
| innate-like cd4 t cells selected by thymocytes suppress adaptive immune responses against bacterial infections. | we have reported a new innate-like cd4 t cell population that expresses cell surface makers of effector/memory cells and produce th1 and th2 cytokines immediately upon activation. unlike conventional cd4 t cells that are selected by thymic epithelial cells, these cd4 t cells, named t-cd4 t cells, are selected by mhc class ii expressing thymocytes. previously, we showed that the presence of t-cd4 t cells protected mice from airway inflammation suggesting an immune regulatory role of t-cd4 t cells ... | 2011 | 23264931 |
| mitogenic response and probiotic characteristics of lactic acid bacteria isolated from indigenously pickled vegetables and fermented beverages. | lactic acid bacteria from indigenous pickled vegetables and fermented beverages (fermented rice and madhuca longifolia flowers) were isolated and investigated for their functional characteristics in vitro as potential new probiotic strains. four isolates (all lactobacillus spp.) selected on the basis of high tolerance to bile (0.2%) were identified by standard and molecular methods (16s rdna) as l. helveticus, l. casei, l. delbrueckii and l. bulgaricus from pickled vegetables and fermented bever ... | 2011 | 22806866 |
| the cultivation of the mushroom agaricus bisporus (champignon): micro-organisms and preservability. | cultivation of agaricus bisporus mushrooms requires the use of substrates that are potentially dangerous from the microbiological point of view, such as chicken and horse manure. microorganisms can pose risks to consumers and workers, and generate lower profits. packaging of fresh mushrooms with impermeable films is used to extend their shelf life but creates anaerobic and humidity conditions that could favour the growth of microorganisms such as listeria monocytogenes and clostridium botulinum. ... | 2011 | 22508614 |
| [infectious encephalitis]. | infectious encephalitis is a severe disease of the central nervous system with high case fatality ratio and long-term sequels. although a high proportion remains unexplained, the most frequently identified cause in all countries was always herpes simplex virus type 1. the generalisation of acyclovir dramatically improved the survival rate of herpes simplex encephalitis patients. however, a high proportion of patients still experience neuropsychological sequels. bacteria are a usual cause of ence ... | 2011 | 22288343 |
| [establishment of a multiplex pcr detection method for four foodborne pathogens]. | to establish a multiplex pcr method for simultaneous detection of salmonella, shigella, staphylococcus aureus and listeria monocytogenes in food. | 2011 | 22279674 |
| loop-mediated amplification accelerated by stem primers. | isothermal nucleic acid amplifications (inaats) have become an important alternative to pcr for in vitro molecular diagnostics in all fields. amongst inaats loop-mediated amplification (lamp) has gained much attention over the last decade because of the simplicity of hardware requirements. lamp demonstrates performance equivalent to that of pcr, but its application has been limited by the challenging primer design. the design of six primers in lamp requires a selection of eight priming sites wit ... | 2011 | 22272122 |
| antimicrobial activity of cyclodextrin entrapped allyl isothiocyanate in a model system and packaged fresh-cut onions. | the aim of this work was to determine the antimicrobial effect of allyl isothiocyanate (ait) entrapped in alpha and beta cyclodextrin inclusion complexes (ics). in model experiments, ait formulations were applied to filter paper discs fixed inside the lid of petri dishes, where the agar surface was inoculated with the target organism (penicillium expansum, escherichia coli or listeria monocytogenes). solid phase microextraction coupled with gas chromatography was used to determine static headspa ... | 2011 | 22265303 |
| type i interferon signaling regulates the composition of inflammatory infiltrates upon infection with listeria monocytogenes. | type i ifn is key to the immune response to viral pathogens, however its role in bacterial infections is less well understood. mice lacking the type i ifn receptor (ifnar-/-) demonstrate enhanced resistance to infection with listeriamonocytogenes. we have now determined that following infection with listeria, the composition of innate cells recruited to the peritoneal cavity of ifnar-/- mice reflects an increase in the frequency of neutrophils and a decrease in monocyte frequency compared to wt ... | 2011 | 22212606 |
| innate ifn-γ-producing cells in the spleen of mice early after listeria monocytogenes infection: importance of microenvironment of the cells involved in the production of innate ifn-γ. | production of innate interferon-γ (ifn-γ) is a crucial step in immunological defense against bacteria. however, there is little information regarding cellular mechanisms underlying ifn-γ production in vivo early after bacterial infection. here we analyze innate ifn-γ production in the spleen of mice early after listeria monocytogenes (lm) infection ex vivo by flow-cytometry and in situ by immunohistochemistry, and compare them with the ifn-γ-producing cells reported previously in our in vitro co ... | 2011 | 22566816 |
| [distribution of listeria monocytogenes serotypes isolated from foods, colombia, 2000-2009]. | listeria monocytogenes is an intracellular, opportunistic pathogen which can cause severe infections such as meningitis, encephalitis and bacteremia. it can also cause abortions in human beings. foods are the vehicle for infection of the host. serotypification has discriminated 13 serotypes: 1/2a,1/2b, 1/2c, 3a, 3b, 3c, 4a, 4ab, 4b, 4c, 4d, 4e, 7. 4b is the cause of the majority of cases of listeriosis in the world. | 2012 | 23715189 |
| [outbreaks of salmonella spp., staphylococcus aureus and listeria monocytogenes associated with poultry consumption. systematic review]. | food borne diseases are a serious public health problem. poultry are often associated with these outbreaks. | 2012 | 23715186 |
| [in vitro activity of doripenem against strains from pediatric diseases and strains causing purulent meningitis]. | this study evaluated the in vitro activity of doripenem (drpm) against 200 streptococcus pneumoniae and 197 haemophilus influenzae from children and adults in 2007, 50 h. influenzae type b in 2006, 20 listeria monocytogenes in 1990-2005, 23 neisseria meningitidis in 2007-2009 and 83 bordetella pertussis in 1989-2003. all strains were isolated from japanese clinical facilities. we also investigated in vitro activity of other carbapenems (meropenem, imipenem, panipenem, biapenem), cephems (ceftria ... | 2012 | 23593734 |
| lipid components and water soluble metabolites in salted and dried tuna (thunnus thynnus l.) roes. | the salted and dried product of tuna roe (bottarga) is a seafood characteristic of the mediterranean area and exported all over the world. samples of bottarga from bluefin tunas (thunnus thynnus, l.) caught in the southwest mediterranean sea were analysed. the samples were characterised by high content of marine wax esters (55-67 mol% of lipid classes), of docosahexaenoic (22:6 n-3, 25 w%) and oleic (18:1 n-9, 19 w%) fatty acids. cholesterol was detected as 7-9 w% of lipids. free fatty acids, in ... | 2012 | 23497865 |
| [protective immune responses induced by a recombinant listeria monocytogenes delivering hpv16 e7]. | to investigate specific immune responses elicited by a recombinant listeria monocytogenes strain lm4 deltahly::e7 and assess protective effect in c57bl/6 mice. | 2012 | 23457800 |
| effect of soy and milk protein-related compounds on listeria monocytogenes infection in human enterocyte caco-2 cells and a/j mice. | listeria monocytogenes causes listeriosis in humans, mainly through the consumption of ready-to-eat foods such as cheese. immunocompromised persons, the elderly, and pregnant women and their fetuses or newborns are at the highest risk for the infection. we examined the effects of dietary milk-casein (mc) and soy-protein (sp), and their digested compounds tryptone (tp) and phytone peptone (pp), respectively, on l. monocytogenes invasion and infection in human enterocyte-like caco-2 cells and a/j ... | 2012 | 23442612 |
| microbiological investigation of raphanus sativus l. grown hydroponically in nutrient solutions contaminated with spoilage and pathogenic bacteria. | the survival of eight undesired (spoilage/pathogenic) food related bacteria (citrobacter freundii pss60, enterobacter spp. pss11, escherichia coli pss2, klebsiella oxytoca pss82, serratia grimesii pss72, pseudomonas putida pss21, stenotrophomonas maltophilia pss52 and listeria monocytogenes atcc 19114(t)) was investigated in mineral nutrient solution (mns) during the crop cycle of radishes (raphanus sativus l.) cultivated in hydroponics in a greenhouse. mnss were microbiologically analyzed weekl ... | 2012 | 23290244 |
| intrinsic role of foxo3a in the development of cd8+ t cell memory. | cd8(+) t cells undergo rapid expansion during infection with intracellular pathogens, which is followed by swift and massive culling of primed cd8(+) t cells. the mechanisms that govern the massive contraction and maintenance of primed cd8(+) t cells are not clear. we show in this study that the transcription factor, foxo3a, does not influence ag presentation and the consequent expansion of cd8(+) t cell response during listeria monocytogenes infection, but plays a key role in the maintenance of ... | 2012 | 23277488 |
| micro-rna 155 is required for optimal cd8+ t cell responses to acute viral and intracellular bacterial challenges. | recent studies have begun to define the role of micro-rnas in regulating the immune response. micro-rna155 (mir-155) has been shown to play a role in germinal center formation, t cell inflammation, and regulatory t cell development. in this study, we evaluated the role of mir-155 in cytotoxic t cell function. we report in this study that mice lacking mir-155 have impaired cd8(+) t cell responses to infections with lymphocytic choriomeningitis virus and the intracellular bacteria listeria monocyt ... | 2012 | 23275599 |
| isolation and characterization of a nisin-like bacteriocin produced by a lactococcus lactis strain isolated from charqui, a brazilian fermented, salted and dried meat product. | a lactococcus lactis subsp. lactis strain (l. lactis 69) capable to produce a heat-stable bacteriocin was isolated from charqui, a brazilian fermented, salted and sun-dried meat product. the bacteriocin inhibited, in vitro, listeria monocytogenes, staphylococcus aureus, several lactic acid bacteria isolated from foods and spoilage halotolerant bacteria isolated from charqui. the activity of the bacteriocin was not affected by ph (2.0-10.0), heating (100 °c), and chemical agents (1% w/v). treatme ... | 2012 | 23273471 |
| the insect galleria mellonella as a powerful infection model to investigate bacterial pathogenesis. | the study of bacterial virulence often requires a suitable animal model. mammalian models of infection are costly and may raise ethical issues. the use of insects as infection models provides a valuable alternative. compared to other non-vertebrate model hosts such as nematodes, insects have a relatively advanced system of antimicrobial defenses and are thus more likely to produce information relevant to the mammalian infection process. like mammals, insects possess a complex innate immune syste ... | 2012 | 23271509 |
| inhibitory activity of surfactin, produced by different bacillus subtilis subsp. subtilis strains, against listeria monocytogenes sensitive and bacteriocin-resistant strains. | three surfactin-producing bacillus subtilis strains, c4, m1 and g2iii, previously isolated from honey and intestines from the apis mellifera l. bee, were phylogenetically characterized at sub-species level as b. subtilis subsp. subtilis using gyra gene sequencing. the antagonistic effect of surfactin was studied against seven different listeria monocytogenes strains, 6 of which were resistant to bacteriocins. surfactin showed anti-listeria activity against all 7 strains and a dose of 0.125 mg/ml ... | 2012 | 23265790 |
| synthesis, pharmacological activity evaluation and molecular modeling of new polynuclear heterocyclic compounds containing benzimidazole derivatives. | novel heterocyclic compounds containing benzimidazole derivatives were synthesized from 2-(1h-benzimidazol-2-yl) acetonitrile (1) and arylhydrazononitrile derivative 2 was obtained via coupling of 1 with 4-methyl phenyldiazonium salt, which was then reacted with hydroxylamine hydrochloride to give amidooxime derivative 3. this product was cyclized into the corresponding oxadiazole derivative 4 upon reflux in acetic anhydride. compound 4 was refluxed in dmf in the presence of triethylamine to giv ... | 2012 | 23263801 |
| elaboration of highly hydrophobic polymeric surface - a potential strategy to reduce the adhesion of pathogenic bacteria? | different polymeric surfaces have been modified in order to reach a high hydrophobic character, indeed the superhydrophobicity property. for this purpose, polypropylene and polystyrene have been treated by rf or μwaves cf4 plasma with different volumes, the results were compared according to the density of injected power. the effect of pretreatment such as mechanical abrasion or plasma activation was also studied. the modified surfaces were shown as hydrophobic, or even superhydrophobic dependin ... | 2012 | 23827554 |
| immunological response in egg-sensitive adults challenged with cheese containing or not containing lysozyme. | lysozyme is an enzyme that hydrolyzes bacterial peptidoglicans. for this reason, it is used in cheese manufacturing in order to prevent a defect of long-ripened hard cheese called "late blowing" due to the outgrowth of spores of clostridium tyrobutyricum and clostridium butyricum. moreover, germination of listeria monocytogenes spores into vegetative cells is also sensitive to lysozyme. the enzyme can be an allergenic molecule, and for this reason there are concerns about its use in food industr ... | 2012 | 23756581 |
| considerations on the isolation of listeria monocytogenes and other listeria spp. in food products. | listeria monocytogenes, a gram positive bacillus, is well adapted for survival as a saprophyte in soil and decaying vegetation, but also able to cause serious infections, due to its ability of intracellular multiplication and meningeal and placental dissemination. a total number of 43 listeria spp. strains isolated from food were investigated. bacterial identification was performed according to standard methodology, based on: gram staining affinity, morphology, culture aspects on blood agar (exa ... | 2012 | 23755698 |
| survival of listeria monocytogenes, and other food spoilage microbes in vacuum packaged west african soft cheese 'wara'. | 'wara' soft cheese is traditionally produced in nigeria and has a poor microbial quality. this study assessed the survivability of listeria monocytogenes and other food spoilage microbes (enterobacteriacea, molds and yeasts) in vacuum packaged soft cheese treated independently with carica papaya (vcpc), terminalia cattapa (vtcc) crude extracts, nisin (vnc), and the combination of these three treatments (v+3) stored at 15 degrees c and 28 degrees c for a three week storage period. vacuum packagin ... | 2012 | 23678645 |
| prevalence of listeria monocytogenes in idiazabal cheese. | raw-milk cheese has been identified in risk assessment as a food of greater concern to public health due to listeriosis. | 2012 | 23588469 |
| simultaneous detection of listeria monocytogenes and salmonella spp. in dairy products using real time pcr-melt curve analysis. | the present investigation reports development of post real time pcr (rti-pcr) - melt curve analysis for simultaneous detection of listeria monocytogenes and salmonella spp. the optimal sybr green i (sg-i) concentration of 1.6 μm resulted in two specific peaks with melting temperature (tm) of 79.90 ± 0.39 °c and 86.29 ± 0.13 °c for l. monocytogenes and salmonella spp respectively. the detection sensitivity of the assay in reconstituted non-fat dried milk (nfdm; 11%) spiked with the target pathoge ... | 2011 | 23572847 |
| how does listeria monocytogenes combat acid conditions? | listeria monocytogenes, a major foodborne pathogen, possesses a number of mechanisms that enable it to combat the challenges posed by acidic environments, such as that of acidic foods and the gastrointestinal tract. one mechanism employed by l. monocytogenes for survival at low ph is the adaptive acid tolerance response (atr) in which a short adaptive period at a nonlethal ph induces metabolic changes that allow the organism to survive a lethal ph. overcoming acid conditions by l. monocytogenes ... | 2012 | 23540331 |
| growth kinetics of listeria monocytogenes and spoilage microorganisms in fresh-cut cantaloupe. | the main objective of this study was to investigate the growth kinetics of listeria monocytogenes and background microorganisms in fresh-cut cantaloupe. fresh-cut cantaloupe samples, inoculated with three main serotypes (1/2a, 1/2b, and 4b) of l. monocytogenes, were incubated at different temperatures, ranging from 4 to 43 °c, to develop kinetic growth models. during storage studies, the population of both background microorganisms and l. monocytogenes began to increase almost immediately, with ... | 2012 | 23498195 |
| fate of escherichia coli o157:h7, listeria monocytogenes, and salmonella on fresh-cut celery. | illnesses from escherichia coli o157:h7, listeria monocytogenes, and salmonella have been associated with the consumption of numerous produce items. little is known about the effect of consumer handling practices on the fate of these pathogens on celery. the objective of this study was to determine pathogen behavior at different temperatures under different storage conditions. commercial fresh-cut celery was inoculated at ca. 3logcfu/g onto either freshly cut or outer uncut surfaces and stored i ... | 2012 | 23498192 |
| high content of biogenic amines in pecorino cheeses. | pecorino refers to italian cheeses made exclusively from raw or pasteurized ewes' milk, characterized by a high content of fat matter and it is mainly produced in the middle and south of italy by traditional procedures. the autochthonous microbiota plays an important role in the organoleptic traits of pecorino cheese and it can influence biogenic amines (ba) content. the aim of this study was to characterize from microbiological and chemical point of view 12 randomly purchased commercial cheeses ... | 2012 | 23498190 |
| [bacteriostatic and/or bactericidal extract of aloe vera gel on cultures of listeria monocytogenes]. | listeria monocytogenes is a bacteria responsible for food borne diseases (fbd). the effect of aloe vera gel extract as a possible bacteriostatic and/or bactericidal against listeria monocytogenes, was checked by determined the minimum inhibitory concentration (mic), the time of minimum inhibition (tmi) and minimum bactericidal concentration (mbc) solutions extract of aloe vera gel in different concentrations on cultures of listeria monocytogenes atcc 7635. we applied the agar diffusion method, u ... | 2012 | 23477211 |
| enterocin t, a novel class iia bacteriocin produced by enterococcus sp. 812. | enterococcus sp. 812, isolated from fresh broccoli, was previously found to produce a bacteriocin active against a number of gram-positive bacteria, including listeria monocytogenes. bacteriocin activity decreased slightly after autoclaving (121 °c for 15 min), but was inactivated by protease k. mass spectrometry analysis revealed the bacteriocin mass to be approximately 4,521.34 da. n-terminal amino acid sequencing yielded a partial sequence, nh2-atyygngvyxdkkkxwvewgqa, by edman degradation, wh ... | 2013 | 23912616 |
| antibody-based sensors: principles, problems and potential for detection of pathogens and associated toxins. | antibody-based sensors permit the rapid and sensitive analysis of a range of pathogens and associated toxins. a critical assessment of the implementation of such formats is provided, with reference to their principles, problems and potential for 'on-site' analysis. particular emphasis is placed on the detection of foodborne bacterial pathogens, such as escherichia coli and listeria monocytogenes, and additional examples relating to the monitoring of fungal pathogens, viruses, mycotoxins, marine ... | 2009 | 22408533 |
| clues to diagnosing culture negative listeria rhombencephalitis. | a previously healthy 35-year-old caucasian woman developed left body (including facial) hemianaesthesia, asymmetrical lower cranial nerve palsies and cerebellar signs after a 4-day history of headache, nausea and vomiting. serial blood and cerebrospinal fluid (csf) cultures returned negative for a culprit organism. csf examination revealed a lymphocytic pleocytosis and an elevated protein count. csf cytological examination identified plasma cells. mri of brain showed multiple ring-enhancing 'abs ... | 2012 | 23035165 |
| bilateral optic perineuritis as the presenting feature of giant cell arteritis. | optic perineuritis (opn) is an uncommon inflammatory disorder of the optic nerve sheath. most cases are idiopathic, though opn can rarely occur as a manifestation of vasculitic diseases. we describe the case of a 74-year-old caucasian man presenting with diplopia and bilateral visual loss. a brain mri showed bilateral opn without involvement of other structures. all the tests performed to investigate opn's aetiology showed negative results. considering clinical presentation and neuroimaging find ... | 2013 | 23365154 |
| dexd-box rna-helicases in listeria monocytogenes are important for growth, ribosomal maturation, rrna processing and virulence factor expression. | rna-helicases are proteins required for the unwinding of occluding secondary rna structures, especially at low temperatures. in this work, we have deleted all 4 dexd-box rna helicases in various combinations in the gram-positive pathogen listeria monocytogenes. our results show that 3 out of 4 rna-helicases were important for growth at low temperatures, whereas the effect was less prominent at 37°c. over-expression of one rna-helicase, lmo1450, was able to overcome the reduced growth of the quad ... | 2014 | 25590644 |
| rna helicase important for listeria monocytogenes hemolytic activity and virulence factor expression. | rna helicases have been shown to be important for the function of rna molecules at several levels, although their putative involvement in microbial pathogenesis has remained elusive. we have previously shown that listeria monocytogenes dexd-box rna helicases are important for bacterial growth, motility, ribosomal maturation, and rrna processing. we assessed the importance of the rna helicase lmo0866 (here named csha) for expression of virulence traits. we observed a reduction in hemolytic activi ... | 2015 | 26483402 |
| an hd-domain phosphodiesterase mediates cooperative hydrolysis of c-di-amp to affect bacterial growth and virulence. | the nucleotide cyclic di-3',5'- adenosine monophosphate (c-di-amp) was recently identified as an essential and widespread second messenger in bacterial signaling. among c-di-amp-producing bacteria, altered nucleotide levels result in several physiological defects and attenuated virulence. thus, a detailed molecular understanding of c-di-amp metabolism is of both fundamental and practical interest. currently, c-di-amp degradation is recognized solely among dhh-dhha1 domain-containing phosphodiest ... | 2015 | 25583510 |
| targeting of the central nervous system by listeria monocytogenes. | among bacteria that reach the central nervous system (cns), listeria monocytogenes (lm) is one of deadliest, in human and ruminant. this facultative intracellular bacterium has the particularity to induce meningitis, meningoencephalitis and rhombencephalitis. mechanisms by which lm accesses the cns remain poorly understood, but two major routes of infection have been proposed, based on clinical, in vitro and in vivo observations. a retrograde neural route is likely to occur in ruminants upon cro ... | 2012 | 22460636 |
| ruptured thoracic aortic aneurysm infected with listeria monocytogenes: a case report and a review of literature. | a 75-year-old male with a history of alcoholic liver cirrhosis, sigmoid colon cancer, and metastatic liver cancer was admitted to our institution with a complaint of a prickly feeling in his chest. on admission, a chest radiograph revealed a normal cardio-thoracic ratio of 47%. echocardiography revealed pericardial effusion and blood chemical analyses revealed elevated c-reactive protein levels (14.7 mg/dl). on day 3, chest radiography revealed cardiomegaly with a cardio-thoracic ratio of 58% an ... | 2013 | 25512697 |
| intracellular organisms as placental invaders. | in this article we present a novel model for how the human placenta might get infected via the hematogenous route. we present a list of diverse placental pathogens, like listeria monocytogenes or cytomegalovirus, which are familiar to most obstetricians, but others, like salmonella typhi, have only been reported in case studies or small case series. remarkably, all of these organisms on this list are either obligate or facultative intracellular organisms. these pathogens are able to enter and su ... | 2015 | 27695204 |
| quantification of bacterial indicators and zoonotic pathogens in dairy wastewater ponds. | zoonotic pathogens in land-applied dairy wastewaters are a potential health risk. the occurrence and abundance of 10 pathogens and 3 fecal indicators were determined by quantitative real-time pcr (qpcr) in samples from 30 dairy wastewaters from southern idaho. samples tested positive for campylobacter jejuni, stx(1)- and eaea-positive escherichia coli, listeria monocytogenes, mycobacterium avium subsp. paratuberculosis, and salmonella enterica, with mean recoveries of genomic dna corresponding t ... | 2012 | 22983964 |
| a multiplex pcr/ldr assay for simultaneous detection and identification of the niaid category b bacterial food and water-borne pathogens. | enteric pathogens that cause gastroenteritis remain a major global health concern. the goal of this study was to develop a multiplex pcr/ligation detection reaction (ldr) assay for the detection of all niaid category b bacterial food and water-borne pathogens directly from stool specimens. to validate the pcr/ldr assay, clinical isolates of campylobacter spp., vibrio spp., shigella spp., salmonella spp., listeria monocytogenes, yersinia enterocolitica, and diarrheagenic escherichia coli were tes ... | 2014 | 24709368 |
| multiplex detection of nine food-borne pathogens by mpcr and capillary electrophoresis after using a universal pre-enrichment medium. | routine microbiological quality analyses in food samples require, in some cases, an initial incubation in pre-enrichment medium. this is necessary in order to ensure that small amounts of pathogenic strains are going to be detected. in this work, a universal pre-enrichment medium has been developed for the simultaneous growth of bacillus cereus, campylobacter jejuni, clostridium perfringens, cronobacter sakazakii, escherichia coli, enterobacteriaceae family (38 species, 27 genera), listeria mono ... | 2015 | 26579100 |
| development of a droplet digital polymerase chain reaction for rapid and simultaneous identification of common foodborne pathogens in soft cheese. | dairy products can harbor various microorganisms (e.g., campylobacter spp., salmonella spp., listeria monocytogenes, verocytotoxin-producing escherichia coli) arising from animal reservoirs, and which can become important sources of foodborne illness. therefore, early detection of food pathogens is crucial to prevent diseases. we wished to develop an accurate quantitative protocol based on a droplet digital polymerase chain reaction (ddpcr) involving eight individual taqman™ reactions to detect ... | 2016 | 27840628 |
| persistence and potential viable but non-culturable state of pathogenic bacteria during storage of digestates from agricultural biogas plants. | despite the development of on-farm anaerobic digestion as a process for making profitable use of animal by-products, factors leading to the inactivation of pathogenic bacteria during storage of digestates remain poorly described. here, a microcosm approach was used to evaluate the persistence of three pathogenic bacteria (salmonella enterica derby, campylobacter coli and listeria monocytogenes) in digestates from farms, stored for later land spreading. nine samples, including raw digestates, liq ... | 2016 | 27695451 |
| fate of viable but non-culturable listeria monocytogenes in pig manure microcosms. | the fate of two strains of listeria monocytogenes and their ability to become viable but non-culturable (vbnc) was investigated in microcosms containing piggery effluents (two raw manures and two biologically treated manures) stored for 2 months at 8 and 20°c. levels of l. monocytogenes were estimated using the culture method, qpcr, and propidium monoazide treatment combined with qpcr (qpcrpma). the chemical composition and the microbial community structure of the manures were also analyzed. the s ... | 2016 | 26973623 |
| survey of infectious etiologies of bovine abortion during mid- to late gestation in dairy herds. | bovine abortion of unknown infectious etiology still remains a major economic problem. thus, we investigated whether brucella spp., listeria monocytogenes, salmonella spp., campylobacter spp. and coxiella burnetii are associated with abortion and/or stillbirth in tunisian dairy cattle. using a pan-chlamydiales pcr, we also investigated the role of chlamydiaceae, waddlia chondrophila, parachlamydia acanthamoebae and other members of the chlamydiales order in this setting. veterinary samples taken ... | 2014 | 24662769 |
| use of essential oils and extracts from spices in meat protection. | the hydro distillation method was used in this study to get essential oils (eos) from cumin (cuminum cyminum l.), clove (eugenia caryohyllata) and elecampane (inula helenium l.) and the co-hydro distillation method (addition of fatty acid ethyl esters as extraction cosolvents) to get functional extracts (efs). the mic (minimum inhibitory concentration) and the mbc (minimum bactericidal concentration) were determined on five pathogenic strains (escherichia coli o157:h7, salmonella, listeria monoc ... | 2011 | 24803704 |
| bacteriophage biocontrol of listeria monocytogenes on soft ripened white mold and red-smear cheeses. | soft-ripened cheeses belong to the type of food most often contaminated with listeria monocytogenes, and they have been implicated in several outbreaks of listeriosis. bacteriophages represent an attractive way to combat foodborne pathogens without affecting other properties of the food. we used the broad host range, virulent listeria phage a511 for control of l. monocytogenes during the production and ripening phases of both types of soft-ripened cheeses, white mold (camembert-type) cheese, as ... | 2011 | 22334865 |