| a note on the incidences of salmonella spp., listeria spp. and escherichia coli o157:h7 serotypes in turkish sausage (soudjouck). | the incidence of salmonella spp., listeria monocytogenes and escherichia coli o157:h7 was determined in 100 turkish sausage (soudjouck) samples collected from shops and markets in the afyon province, turkey. salmonella spp. were detected in 7% of the samples. all of the isolates were s. enterica paratyphi b. in addition, listeria spp. were detected in 9% of the samples. its distribution was 7% l. monocytogenes and 1% each of l. ivanovii and l. innocua. serological study of the seven l. monocytog ... | 2006 | 22061388 |
| characterization of lactic acid bacteria isolated from a greek dry-fermented sausage in respect of their technological and probiotic properties. | a total of 147 lactic acid bacteria was isolated from two types of naturally fermented dry sausages at four different stages of the ripening process studied in order to select the most suitable strains according to their technological characteristics including probiotic properties and antimicrobial activity against food-borne pathogens. identification of the isolates revealed that 90% were lactobacilli, 4% enterococci, 3% pediococcus sp. and sporadic isolates of weissella viridescens, leuconosto ... | 2003 | 22063449 |
| [quantitative risk assessment of listeria monocytogenes in bulk cooked meat products]. | to conduct a quantitative risk assessment for listeria monocytogenes in bulk cooked meat products in china. | 2011 | 21914338 |
| comparison of public health impact of listeria monocytogenes product-to-product and environment-to-product contamination of deli meats at retail. | this study compared the relative public health impact in deli meats at retail contaminated with listeria monocytogenes by either (i) other products or (ii) the retail environment. modeling was performed using the risk of listeriosis-associated deaths as a public health outcome of interest and using two deli meat products (i.e., ham and turkey, both formulated without growth inhibitors) as model systems. based on reported data, deli meats coming to retail were assumed to be contaminated at a fre ... | 2011 | 22054186 |
| survival of listeria innocua in dry fermented sausages and changes in the typical microbiota and volatile profile as affected by the concentration of nitrate and nitrite. | the involvement of nitrate and nitrite in the formation of n-nitrosamines in foods is a matter of great concern. this situation has led to revise the real amount of nitrate and nitrite needed in meat products to exert proper technological and safety activities, and also to extensive research to find alternatives to their use. the present study addresses the possibility of reducing the ingoing amounts of these additives below the legal limits established by the current european regulations. diffe ... | 2011 | 22200323 |
| epidemiology and risk management of listeriosis in india. | listeria monocytogenes is a foodborne pathogen that can cause serious invasive illness, mainly in certain well-defined high-risk groups, including elderly and immunocompromised patients, pregnant women, newborns and infants. in india, this pathogen has been isolated from humans, animals and foods. the incidence of listeria is generally comparable to those reported elsewhere in the world. in humans, maternal/neonatal listeriosis is the most common clinical form reported. among animal populations, ... | 2011 | 21955732 |
| the arsenal of virulence factors deployed by listeria monocytogenes to promote its cell infection cycle. | listeria monocytogenes is an intracellular gram-positive pathogen and the etiological agent of listeriosis, a human food-borne disease potentially fatal for certain risk groups. the virulence of l. monocytogenes is supported by a highly complex and coordinated intracellular life cycle that comprises several crucial steps: host cell adhesion and invasion, intracellular multiplication and motility, and intercellular spread. the completion of each stage is dependent on the orchestrated activity of ... | 2011 | 21921683 |
| [listeria monocytogenes osteomyelitis.] | | 2011 | 22153782 |
| microbial infection-induced expansion of effector t cells overcomes the suppressive effects of regulatory t cells via an il-2 deprivation mechanism. | regulatory foxp3(+) t cells are a critical cell population that suppresses t cell activation in response to microbial and viral pathogens. we identify a cell-intrinsic mechanism by which effector cd4(+) t cells overcome the suppressive effects of regulatory t (treg) cells in the context of three distinct infections: toxoplasma gondii, listeria monocytogenes, and vaccinia virus. the acute responses to the parasitic, bacterial, and viral pathogens resulted in a transient reduction in frequency and ... | 2011 | 22147768 |
| effect of food grade organic acids on inoculated s. aureus, l. monocytogenes, e. coli and s. typhimurium in sheep/goat meat stored at refrigeration temperature. | sheep/goat forequarters procured from freshly slaughtered animals were decontaminated with hot water and inoculated with staphylococcus aureus, listeria monocytogenes, escherichia coli and salmonella typhimurium. the forequarters were individually spray washed with 2% lactic acid and 1.5% acetic + 1.5% propionic acid combination. total viable count (tvc) of the treated meat samples was reduced by about 0.52 and 1.16 log units with marginal changes in colour and odour scores. inoculated organisms ... | 2004 | 22061013 |
| inhibitory activity of lactobacillus plantarum lmg p-26358 against listeria innocua when used as an adjunct starter in the manufacture of cheese. | lactobacillus plantarum lmg p-26358 isolated from a soft french artisanal cheese produces a potent class iia bacteriocin with 100% homology to plantaricin 423 and bacteriocidal activity against listeria innocua and listeria monocytogenes. the bacteriocin was found to be highly stable at temperatures as high as 100°c and ph ranges from 1-10. while this relatively narrow spectrum bacteriocin also exhibited antimicrobial activity against species of enterococci, it did not inhibit dairy starters inc ... | 2011 | 21995443 |
| survival of listeria monocytogenes introduced as a post-aging contaminant during storage of low-salt cheddar cheese at 4, 10, and 21°c. | traditional aged cheddar cheese does not support listeria monocytogenes growth and, in fact, gradual inactivation of the organism occurs during storage due to intrinsic characteristics of cheddar cheese, such as presence of starter cultures, salt content, and acidity. however, consuming high-salt (sodium) levels is a health concern and the dairy industry is responding by creating reduced-salt cheeses. the microbiological stability of low-salt cheese has not been well documented. this study exami ... | 2011 | 21854905 |
| inhibition of listeria monocytogenes by a bacteriocinogenic lactobacillus sake strain in modified atmosphere-packaged brazilian sausage. | lactobacillus sake 2a is a bacteriocinogenic strain isolated from "lingüiça frescal", a brazilian sausage. the combined effect of modified-atmosphere (ma) packaging (100% co(2) and 50% co(2)/50% n(2)) and addition of l. sake 2a on inhibition of growth of listeria monocytogenes was evaluated in "lingüiça" stored at 6 °c. by the end of the first week, the inhibition of l. monocytogenes due to ma was significant (p?0.05) while the presence of l. sake 2a did not influence significantly the growth of ... | 2002 | 22061076 |
| listeria monocytogenes encephalitis in france. | the authors had for aim to describe the characteristics of listeriosis encephalitis patients enrolled in a national study. | 2011 | 22036519 |
| human cd8+ t cells display a differential ability to undergo cytokine-driven bystander activation. | a subset of cd44(hi)cd8+ t cells in some, but not all mice, can be induced to rapidly secrete ifnγ during infection with listeria monocytogenes. this response is dependent on the presence of both il-12 and il-18 and does not require engagement of the t cell receptor. in this study, we demonstrate that human cd8+ t cells also vary widely in their ability to secrete ifnγ within 15h of either listeria infection or cytokine stimulation. the magnitude of the rapid ifnγ response correlated more closel ... | 2011 | 21978649 |
| pH dependence of listeriolysin O aggregation and pore-forming ability. | Listeriolysin O (LLO) is the major factor implicated in the escape of Listeria monocytogenes from the phagolysosome. It is the only representative of cholesterol-dependent cytolysins that exhibits pH-dependent activity. Despite intense studies of LLO pH-dependence, this feature of the toxin still remains incompletely explained. Here we used fluorescence and CD spectroscopy to show that the structure of LLO is not detectably affected by pH at room temperature. We observed slightly altered haemoly ... | 2012 | 22023160 |
| effect of a bacteriocin produced by pediococcus acidilactici against listeria monocytogenes and clostridium perfringens on spanish raw meat. | the inhibitory effect of a bacteriocin, produced by pediococcus acidilactici, against listeria monocytogenes and clostridium perfringens on spanish raw meat surface, was evaluated by in situ assays. samples were incubated with the bacteriocin and then with a culture of the pathogenic bacteria. the treatment with 500, 1000 or 5000 bacteriocin units/ml (bu/ml) reduced the counts of l. monocytogenes after storage at 15°c during 72h by 1, 2 or 3 log cycles and with 1000 or 5000 bu/ml after storage a ... | 2006 | 22061374 |
| evaluation of sodium lactate as a replacement for conventional chemical preservatives in comminuted sausages inoculated with listeria monocytogenes. | sodium lactate (sl) as a potential replacer for potassium sorbate (ps) or sodium benzoate (sb) in comminuted sausages was evaluated. sausages manufactured with 3.3% sl were compared with a control and 0.05 or 0.1% of ps and sb with regard to its influence on changes of chemical composition, physico-chemical and textural properties, and the growth of inoculated listeria monocytogenes (lm) stored at 4 °c for up to 8 weeks. the sausages contained 62-64% moisture, 15-17% fat and 12-14% protein with ... | 2003 | 22063246 |
| diagnosis of listeria monocytogenes meningoencephalitis by real-time pcr for the hly gene. | listeria monocytogenes is a bacterial pathogen that can invade the central nervous system (cns), causing meningoencephalitis and brain abscesses. the diagnosis of cns listeriosis, based on the isolation of the bacteria in the cerebrospinal fluid (csf), can be difficult because of previous antibiotic treatment and a low number of bacteria in the csf. to improve the sensitivity of microbiological diagnosis, we have developed a real-time pcr assay for detecting and quantifying l. monocytogenes dna ... | 2011 | 21918022 |
| the high prevalence of listeria monocytogenes peritonitis in cirrhotic patients of an egyptian medical center. | spontaneous bacterial peritonitis (sbp) is a potentially lethal complication of cirrhosis. it is probably the most characteristic infectious complication of cirrhosis. | 2011 | 22000850 |
| inhibition of listeria monocytogenes by lactobacillus sake strains of meat origin. | the ability of two lactobacillus sake strains of meat origin to inhibit the growth of listeria monocytogenes at 4, 8, 15, 24 and 32°c in a conventional liquid media was investigated. growth of l. monocytogenes was affected by lac. sake strains at all temperatures. the inhibition was higher at 15, 24 and 32°c than at refrigeration temperatures. the inhibitory activity of both lactobacilli was similar perhaps due to the fact that lac. sake 148 produces a bacteriocin inhibitory to l. monocytogenes, ... | 1994 | 22059605 |
| rapid and sensitive detection of listeria monocytogenes by loop-mediated isothermal amplification. | loop-mediated isothermal amplification (lamp) was designed for detection of listeria monocytogenes, which is an important food-borne kind of pathogenic bacteria causing human and animal disease. the primers set for the hlya gene consist of six primers targeting eight regions on specific gene. the lamp assay could be performed within 40 min at 65°c in a water bath. amplification products were visualized by calcein and manganous ion and agarose gel electrophoresis. sensitivity of the lamp assay fo ... | 2011 | 21935669 |
| inhibition of salmonella sp. listeria monocytogenes and staphylococcus aureus in cooked ham by combining antimicrobials, high hydrostatic pressure and refrigeration. | recontamination of ready-to-eat products such as cooked ham during post-processing may be the cause of outbreaks of food-borne disease. the effectiveness of the combination of high pressure processing (hpp) at 600mpa with the natural antimicrobials nisin and potassium lactate has been evaluated in sliced cooked ham spiked with 4logcfu/g of salmonella sp., listeria monocytogenes and staphylococcus aureus after 3-months of storage at 1 and 6°c. in non-hpp sliced cooked ham, the addition of nisin p ... | 2008 | 22062095 |
| the occurrence of listeria monocytogenes in imported ready-to-eat foods in japan. | quantitative analyses of listeria monocytogenes in imported ready-to-eat (rte) foods sold at retail stores in japan were performed. of the 77 non-cooked meat products, 6 samples (7.8%) tested positive. the levels of contamination of 4 of the samples were below 100 colony-forming units (cfu)/g, which is the microbiological criterion for l. monocytogenes in rte foods as determined by codex. however, listeria cells at levels of 100 and 400 cfu/g were detected in a salami sample and a raw ham sample ... | 2011 | 22041477 |
| antibacterial activity of reactive quaternary ammonium compounds in solution and in nonleachable coatings. | antibacterial polymers suitable for coating applications without leaching of the biocidal component have been obtained by uv copolymerization of acrylic resins with acrylic monomers containing quaternary ammonium moieties. suitable reactive biocides, based on quaternary ammonium monomers (qams), endowed with undecylacryloyl group and alkyl chains with 2 (qam-c2), 8 (qam-c8), and 16 (qam-c16) carbon atoms have been synthesized. aqueous solutions of qams showed biocidal activity against escherich ... | 2011 | 22186051 |
| molecular chaperoning by glucose-regulated protein 170 in the extracellular milieu promotes macrophage-mediated pathogen sensing and innate immunity. | recognition of pathogen-associated molecular patterns by innate immune receptors is essential for host defense responses. although extracellular stress proteins are considered as indicators of the stressful conditions (e.g., infection or cell injury), the exact roles of these molecules in the extracellular milieu remain less defined. we found that glucose-regulated protein 170 (grp170), the largest stress protein and molecular chaperone, is highly efficient in binding cpg oligodeoxynucleotides ( ... | 2011 | 22207611 |
| [evaluation of the polymerase chain reaction (pcr) for the detection and identification of listeria monocytogens in fresh cheese coming from the metropolitan area of san josé, costa rica]. | food borne diseases are very important worldwide and their frequency is still high despite the different efforts focused in diminishing their morbidity and mortality. listeria monocytogenes is one of the agents associated in this kind of diseases. in the lactic industry, this bacteria is important since raw milks as well as dairy products have been associated in outbreaks, being fresh cheese one of the most vulnerable products to the contamination with this bacteria. the traditional identificati ... | 2010 | 21866690 |
| Structured Expert Elicitation About Listeria monocytogenes Cross-Contamination in the Environment of Retail Deli Operations in the United States. | Listeria monocytogenes is among the foodborne pathogens with the highest death toll in the United States. Ready-to-eat foods contaminated at retail are an important source of infection. Environmental sites in retail deli operations can be contaminated. However, commonly contaminated sites are unlikely to come into direct contact with food and the public health relevance of environmental contamination has remained unclear. To identify environmental sites that may pose a considerable cross-contami ... | 2011 | 22053817 |
| The species-specific mode of action of the antimicrobial peptide subtilosin against Listeria monocytogenes Scott A. | Aims: To elucidate the molecular mechanism of action of the antimicrobial peptide subtilosin against the foodborne pathogen Listeria monocytogenes Scott A. Methods and Results: Subtilosin was purified from a culture of Bacillus amyloliquefaciens. The minimal inhibitory concentration of subtilosin against L. monocytogenes Scott A was determined by broth microdilution method. The effect of subtilosin on the transmembrane electrical potential (??) and pH gradient (?pH), and its ability to induce ... | 2012 | 22040458 |
| Investigation of the role of ZurR in the physiology and pathogenesis of Listeria monocytogenes. | Listeria monocytogenes is a Gram positive pathogen that is ubiquitous in the environment. It is a facultative anaerobic rod that causes listeriosis, a disease with potentially lethal consequences for susceptible individuals. During infection, the pathogen is capable of sequestering metal ions to act as vital biocatalysts in cellular processes. The zinc uptake regulator (ZurR) is predicted to coordinate uptake of zinc from the external environment. An in-frame deletion of the zurR gene resulted i ... | 2011 | 22133190 |
| protection activity of a novel probiotic strain of bacillus subtilis against salmonella enteritidis infection. | the activity of 240 bacterial isolates screened from the gastrointestinal tracts of native chickens were evaluated for use as a potential probiotic in food animal production in order to protect against animal diseases and reduce pathogenic contamination of human food products. in observing the antagonistic activity of 117 bacilli isolates, 10 of these isolates exhibited higher growth inhibition of seven foodborne pathogens, including salmonella enteritidis, salmonella typhimurium, escherichia co ... | 2011 | 21907374 |
| [Expression and characterization of a new class IIa bacteriocin]. | NB-Cl gene is a potential class IIa bacteriocin gene. To obtain its soluble expression, NB-C1 was fused with the green fluorescent protein (GFP) gene and a recombinant expression vector plVEX 2.4d-GFP-NB-C1 was constructed, which was transformed into Escherichia coli BL21(DE3) pLysS. The expressed fusion protein GFP-NB-CI was purified by Ni-NTA affinity chromatography and the bioactivity was examined using Listeria monocytogenes as the indicator bacteria. The results showed that the expressed fu ... | 2011 | 22016980 |
| analysis of the serine/threonine/tyrosine phosphoproteome of the pathogenic bacterium listeria monocytogenes reveals phosphorylated proteins related to virulence. | phosphorylation is the most common and widely studied post-translational protein modification in bacteria. it plays an important role in all kinds of cellular processes and controls key regulatory mechanisms, including virulence in certain pathogens. to gain insight into the role of protein phosphorylation in the pathogen listeria monocytogenes, the serine (ser), threonine (thr) and tyrosine (tyr) phosphoproteome of this bacterium was determined. we used the "gel free" proteomic approach with hi ... | 2011 | 21956863 |
| illuminating the landscape of host-pathogen interactions with the bacterium listeria monocytogenes. | listeria monocytogenes has, in 25 y, become a model in infection biology. through the analysis of both its saprophytic life and infectious process, new concepts in microbiology, cell biology, and pathogenesis have been discovered. this review will update our knowledge on this intracellular pathogen and highlight the most recent breakthroughs. promising areas of investigation such as the increasingly recognized relevance for the infectious process, of rna-mediated regulations in the bacterium, an ... | 2011 | 22114192 |
| A strange case of waitress headache. | | 2011 | 22098855 |
| Spontaneous bacteriocin resistance in Listeria monocytogenes as a susceptibility screen for identifying different mechanisms of resistance and modes of action by bacteriocins of lactic acid bacteria. | A practical system was devised for grouping bacteriocins of lactic acid bacteria (LAB) based on mode of action as determined by changes in inhibitory activity to spontaneously-acquired bacteriocin resistance (Bac(R)). Wild type Listeria monocytogenes 39-2 was sensitive to five bacteriocins produced by 3 genera of LAB: pediocin PA-1 and pediocin Bac3 (Pediococcus), lacticin FS97 and lacticin FS56 (Lactococcus), and curvaticin FS47 (Lactobacillus). A spontaneous Bac(R) derivative of L. monocytogen ... | 2012 | 22001443 |
| role of general stress-response alternative sigma factors σ(s) (rpos) and σ(b) (sigb) in bacterial heat resistance as a function of treatment medium ph. | this investigation aimed to determine the role of general stress-response alternative sigma factors σ(s) (rpos) and σ(b) (sigb) in heat resistance and the occurrence of sublethal injuries in cell envelopes of stationary-phase escherichia coli bj4 and listeria monocytogenes egd-e cells, respectively, as a function of treatment medium ph. given that microbial death followed first-order inactivation kinetics (r(2)>0.95) the traditional d(t) and z values were used to describe the heat inactivation k ... | 2011 | 22177853 |
| Enterocin CRL35 Inhibits Listeria monocytogenes in a Murine Model. | Abstract Listeria monocytogenes is a foodborne pathogen causative of opportunistic infections. Listeriosis is associated with severe infections in pregnant women causing abortion or neonatal listeriosis. An alternative to antibiotics are safe novel bacteriocins peptides such as enterocin CRL35 with strong antilisterial activity produced by Enterococcus mundtii CRL35. In the present paper, our goal is to study the effectiveness of this peptide and the producer strain in a murine model of pregna ... | 2011 | 22011041 |
| Screening for Listeria monocytogenes surrogate strains applicable to food processing by ultrahigh pressure and pulsed electric field. | Ultrahigh pressure (UHP) and pulsed electric field (PEF) are emerging processing technologies developed to enhance the safety while maintaining the fresh-like quality of food. For each food and process combination, a pathogen of concern (i.e., target pathogen) must be determined, and a low-risk microorganism that serves as the pathogen surrogate for process validation must be identified. The objective of this study was to identify a surrogate for Listeria monocytogenes for UHP and PEF process v ... | 2011 | 22004812 |
| revisiting the protective and pathogenic roles of neutrophils: ly-6g is key! | the physiological and pathophysiological roles of neutrophils in immune homeostasis and disease have been investigated extensively by way of anti-gr-1 mab-mediated depletion experiments; however, the ability of the anti-gr-1 mab to specifically deplete neutrophils has long been questioned and it is now known that this mab, which binds ly6c and ly6g, is also able to deplete monocytes and subsets of cd8(+) t cells. this, therefore, casts doubt on the previous conclusions regarding the role of neu ... | 2011 | 21952813 |
| the pore-forming toxin listeriolysin o mediates a novel entry pathway of l. monocytogenes into human hepatocytes. | intracellular pathogens have evolved diverse strategies to invade and survive within host cells. among the most studied facultative intracellular pathogens, listeria monocytogenes is known to express two invasins-inla and inlb-that induce bacterial internalization into nonphagocytic cells. the pore-forming toxin listeriolysin o (llo) facilitates bacterial escape from the internalization vesicle into the cytoplasm, where bacteria divide and undergo cell-to-cell spreading via actin-based motility. ... | 2011 | 22072970 |
| antilisterial activity of lactic acid bacteria inoculated on cooked ham. | this study was conducted to evaluate the ability of lactobacillus sakei 1, a bacteriocin-producing (bac(+)) lactic acid bacterium (lab), isolated from brazilian fresh pork sausage to inhibit two listeria monocytogenes strains (serotypes 4b and 1/2a) on cooked, sliced vacuum-packaged ham. l. sakei atcc 15521 was used as a non-bacteriocin producer (bac(-)). l. monocytogenes (ca. 2 logcfu/ml) and lab (ca. 6 logcfu/ml) were inoculated on the sterilized ham, vacuum-sealed and incubated at 8°c for 10 ... | 2006 | 22063215 |
| growth of listeria monocytogenes on vacuum-packaged horsemeat for human consumption. | in order to investigate the likelihood of listeria monocytogenes (serotype 4b, atcc 19115) growth on vacuum-packaged horsemeat at refrigeration temperature, fourteen horsemeat surface/volume homogeneous 150 g weight pieces were superficially inoculated with serotype 4b l. monocytogenes and vacuum packaged. the samples were stored at 4±1 °c. two pieces (one for ph determination and one for l. monocytogenes counts) were examined at days 0, 7, 14, 21, 28, 35 and 42. surface ph did not show signific ... | 2004 | 22062545 |
| Tumor-Associated Antigen Expressing Listeria monocytogenes Induces Effective Primary and Memory T-Cell Responses Against Hepatic Colorectal Cancer Metastases. | PURPOSE: Despite advances in therapy for the treatment of metastatic colorectal cancer, many patients die of hepatic disease. Current immunotherapeutic strategies are likely limited by inhibitory signals from the tumor. To successfully eliminate tumor deposits within an organ, an appropriate immunologic milieu to amplify antitumor responses must be developed. METHODS: We used a murine model utilizing the CT26 col ... | 2011 | 21979110 |
| microbiological contamination of digested products from anaerobic co-digestion of bovine manure and agricultural by-products. | this study was performed to investigate the microbiological contamination of digestate product (dp) obtained from the anaerobic co-digestion of bovine manure and agricultural by-products. | 2011 | 21899580 |
| Low concentration of ethylenediaminetetraacetic acid (EDTA) affects biofilm formation of Listeria monocytogenes by inhibiting its initial adherence. | The distribution and survival of the food-borne pathogen Listeria monocytogenes is associated with its biofilm formation ability, which is affected by various environmental factors. Here we present the first evidence that EDTA at low concentration levels inhibits the biofilm formation of L. monocytogenes. This effect of EDTA is not caused by a general growth inhibition, as 0.1 mM EDTA efficiently reduced the biofilm formation of L. monocytogenes without affecting the planktonic growth. Adding 0. ... | 2012 | 22029913 |
| Diverse metabolic profiles of a Streptomyces strain isolated from a hyper-arid environment. | The metabolic profile of Streptomyces sp. strain C34, isolated from the Chilean hyper-arid Atacama Desert soil, is dependent on the culture media used for its growth. The application of an OSMAC approach on this strain using a range of cultivation media resulted in the isolation and identification of three new compounds from the rare class of 22-membered macrolactone polyketides, named chaxalactins A-C (1-3). In addition, the known compounds deferroxamine E (4), hygromycin A (5), and 5?-dihydroh ... | 2011 | 21879726 |
| Prevalence of Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. in Seafood Products Using Multiplex Polymerase Chain Reaction. | Although several etiological agents can be transmitted through seafood consumption, Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. are considered among the most important pathogens in terms of public health and disease. In this study, multiplex polymerase chain reaction (PCR), as a rapid and cost-effective method, was used to determine the prevalence of these pathogens in 245 samples of raw/fresh, frozen, and ready-to-eat (RTE) seafood products market ... | 2011 | 22044288 |
| microbial heat resistance of listeria monocytogenes and the impact on ready-to-eat meat quality after post-package pasteurization. | several methods using bactericides, hydrostatic pressure, and post-package pasteurization technologies to control listeria monocytogenes (lm) in ready-to-eat meats have been attempted. in addition to controlling lm contamination, any newly developed technology must have minimal effects on organoleptic properties. the objectives of this study were to: (1) determine the heat resistance of lm in two brands (a and b) of bologna differing in formulations, and, (2) evaluate the effects of post-package ... | 2006 | 22063046 |
| cd8α(+) dendritic cells are an obligate cellular entry point for productive infection by listeria monocytogenes. | cd8α(+) dendritic cells (dcs) prime cytotoxic t lymphocytes during viral infections and produce interleukin-12 in response to pathogens. although the loss of cd8α(+) dcs in batf3(-/-) mice increases their susceptibility to several pathogens, we observed that batf3(-/-) mice exhibited enhanced resistance to the intracellular bacterium listeria monocytogenes. in wild-type mice, listeria organisms, initially located in the splenic marginal zone, migrated to the periarteriolar lymphoid sheath (pals) ... | 2011 | 21867927 |
| sigb-dependent tolerance to protein synthesis-inhibiting antibiotics in listeria monocytogenes egde. | the alternative sigma factor sigb in food-borne pathogen listeria monocytogenes was determined in this study to be required for tolerance to protein synthesis-inhibiting antibiotics. the minimum inhibitory concentrations of tetracycline hcl and gentamicin sulphate against egdeδsigb were two- and fourfold less than those for egde, respectively. the ability of egdeδsigb to overcome the growth arrest caused by erythromycin and rifampin was also weaker than that of egde. the transcription analysis o ... | 2011 | 22143398 |
| recognition of greater diversity of bacillus species and related bacteria in human faeces. | in a study looking at culturable aerobic actinobacteria associated with the human gastrointestinal tract, the vast majority of isolates obtained from dried human faeces belonged to the genus bacillus and related bacteria. a total of 124 isolates were recovered from the faeces of 10 healthy adult donors. 16s rrna gene sequence analyses showed the majority belonged to the families bacillaceae (n=81) and paenibacillaceae (n=3), with bacillus species isolated from all donors. isolates tentatively id ... | 2011 | 22041546 |
| Long-term mortality in patients diagnosed with Listeria monocytogenes meningitis: A Danish nationwide cohort study. | To determine the long-term mortality, the causes of death and the incidence of cancer in listeria meningitis patients. | 2012 | 22036889 |
| challenges to meat safety in the 21st century. | the safety of meat has been at the forefront of societal concerns in recent years, and indications exist that challenges to meat safety will continue in the future. major meat safety issues and related challenges include the need to control traditional as well as "new," "emerging," or "evolving" pathogenic microorganisms, which may be of increased virulence and low infectious doses, or of resistance to antibiotics or food related stresses. other microbial pathogen related concerns include cross- ... | 2008 | 22062090 |
| Reporter bacteriophage A511::celB transduces a hyperthermostable glycosidase from Pyrococcus furiosus for rapid and simple detection of viable Listeria cells. | Reporter bacteriophages for detection of pathogenic bacteria offer fast and sensitive screening for live bacterial targets. We present a novel strategy employing a gene encoding a hyperthermophilic enzyme, permitting the use of various substrates and assay formats. The celB gene from the hyperthermophilic archaeon Pyrococcus furiosus specifying an extremely thermostable ß-glycosidase was inserted into the genome of the broad host range, virulent Listeria phage A511 by homologous recombination. I ... | 2011 | 22164348 |
| Development and validation of an antigen capture ELISA based on monoclonal antibodies specific for Listeria monocytogenes in food. | A capture enzyme-linked immunosorbent assay (ELISA) for the identification of Listeria monocytogenes in food was standardised and validated. The assay was refined by analysing samples of meat, seafood, dairy products, pasta and flour. The method was found to be 100% specific for Listeria spp. tested, with a limit of sensitivity of 6.6 × 10(3) colony-forming units (cfu)/ml. Comparison of L. monocytogenes capture ELISA against the official International Organization for Standardization (ISO) metho ... | 2011 | 21947967 |
| Ruminant rhombencephalitis-associated Listeria monocytogenes alleles linked to a multilocus variable-number tandem-repeat analysis complex. | Listeria monocytogenes is among the most important food-borne pathogens and is well adapted to persist in the environment. To gain insight into the genetic relatedness and potential virulence of L. monocytogenes strains causing central nervous system (CNS) infections, we used multilocus variable-number tandem-repeat analysis (MLVA) to subtype 183 L. monocytogenes isolates, most from ruminant rhombencephalitis and some from human patients, food, and the environment. Allelic-profile-based comparis ... | 2011 | 21984240 |
| multistate outbreak of listeriosis associated with jensen farms cantaloupe--united states, august-september 2011. | listeriosis is caused by listeria monocytogenes, a gram-positive bacillus common in the environment and acquired by humans primarily through consumption of contaminated food. infection causes a spectrum of illness, ranging from febrile gastroenteritis to invasive disease, including sepsis and meningoencephalitis. invasive listeriosis occurs predominantly in older adults and persons with impaired immune systems. listeriosis in pregnant women is typically a mild "flu-like" illness, but can result ... | 2011 | 21976119 |
| alanine racemase mutants of mycobacterium tuberculosis require d-alanine for growth and are defective for survival in macrophages and mice. | alanine racemase (alr) is an essential enzyme in most bacteria, however some species (e.g. l. monocytogenes) can utilise d-amino acid transaminase (dat) for generating d-alanine, which renders alr non-essential. in addition to the conflicting reports on gene knockout of alr in m. smegmatis, a recent study concluded that depletion of alr does not affect the growth of m. smegmatis. in order to get an unambiguous answer on the essentiality of alr in m. tuberculosis and validate it as a drug target ... | 2011 | 22075031 |
| listeria monocytogenes infection in macrophages induces vacuolar-dependent host mirna response. | listeria monocytogenes is a gram-positive facultative intracellular pathogen, causing serious illness in immunocompromised individuals and pregnant women. upon detection by macrophages, which are key players of the innate immune response against infection, l. monocytogenes induces specific host cell responses which need to be tightly controlled at transcriptional and post-transcriptional levels. here, we ask whether and how host mirnas, which represent an important mechanism of post-transcriptio ... | 2011 | 22114673 |
| selective culling of high avidity antigen-specific cd4+ t cells after virulent salmonella infection. | typhoid fever is a persistent infection caused by host-adapted salmonella strains adept at circumventing immune-mediated host defences. given the importance of t cells in protection, the culling of activated cd4+ t cells after primary infection has been proposed as a potential immune evasion strategy used by this pathogen. we demonstrate that the purging of activated antigen-specific cd4+ t cells after virulent salmonella infection requires spi-2 encoded virulence determinants, and is not restri ... | 2011 | 22044420 |
| diversity of microorganisms in the environment and dry fermented sausages of small traditional french processing units. | naturally fermented sausages produced in nine traditional french processing units and their environmental surfaces were characterised by microbial and physico-chemical analyses. salmonella and staphylococcus aureus were not detected in the environment whereas listeria monocytogenes was detected in four samples. staphylococcus/kocuria, lactic acid bacteria (lab), enterobacteriaceae, pseudomonas, yeasts/moulds and enterococci contaminated the surfaces of two processing units, indicating insufficie ... | 2007 | 22064197 |
| systemic lupus erythematosus (sle) cerebritis versus listeria monocytogenes meningoencephalitis in a patient with systemic lupus erythematosus on chronic corticosteroid therapy: the diagnostic importance of cerebrospinal fluid (csf) of lactic acid levels. | background: listeria monocytogenes is a motile, aerobic, gram-positive intracellular bacillus that causes enteritis, meningitis, meningoencephalitis, or subacute bacterial endocarditis. patients with impaired t-lymphocyte function/cell-mediated immunity are predisposed to intracellular pathogens, e.g., l. monocytogenes. in adults, infection by l. monocytogenes of the central nervous system (cns) clinically manifests as either acute bacterial meningitis or meningoencephalitis. in patients with sy ... | 2011 | 22177759 |
| the survival of e. coli o157:h7, s. typhimurium and l. monocytogenes in black carrot (daucus carota) juice. | in this study the survival and growth patterns of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in various concentrations of black carrot juice were investigated during incubation period at 4°c and 37°c for 7days. several parameters, such as juice concentration (%), ph, incubation temperature (°c) and time (days) were found effective on the survival of pathogens tested. although l. monocytogenes has been found to be the less resistant microorganism to the variable c ... | 2011 | 22153385 |
| new mild technologies in meat processing: high pressure as a model technology. | as a consequence of market globalization, the production and manufacture of meat products is at a stage of innovative dynamics. consumers demand high quality and convenient meat products, with natural flavour and taste, and very much appreciate the fresh appearance of minimally processed food. to harmonize or to blend all these demands without compromising safety, it is necessary to implement new preservation technologies in the meat industry and in the food industry in general. high hydrostatic ... | 2002 | 22061612 |
| effect of packaging and storage time on survival of listeria monocytogenes on kippered beef steak and turkey tenders. | the objective of our study was to determine effect of packaging method and storage time on reducing listeria monocytogenes in shelf-stable meat snacks. commercially available kippered beef steak strips and turkey tenders were dipped into a 5-strain l. monocytogenes cocktail, and dried at 23 °c until a water activity of 0.80 was achieved. inoculated samples were packaged with 4 treatments: (1) vacuum, (2) nitrogen flushed with oxygen scavenger, (3) heat sealed with oxygen scavenger, and (4) heat ... | 2011 | 22133033 |
| Comparison of techniques to screen and characterize bacteria-specific hybridomas for high-quality monoclonal antibodies selection. | Antibodies are very important materials for diagnostics. A rapid and simple hybridoma screening method will help in delivering specific monoclonal antibodies. In this study, we systematically developed the first antibody array to screen for bacteria-specific monoclonal antibodies using Listeria monocytogenes as a bacteria model. The antibody array was developed to expedite the hybridoma screening process by printing hybridoma supernatants on a glass slide coated with an antigen of interest. This ... | 2011 | 22033288 |
| Hyicin 3682, a bioactive peptide produced by Staphylococcus hyicus 3682 with potential applications for food preservation. | Bacteriocins are peptides produced by bacteria and having inhibitory activity against other bacteria. Many of these substances may be useful as antibacterial agents for practical applications. In this study, 21 Staphylococcus spp. isolated from pigs, dogs and bovine milk in different states of Brazil were investigated for staphylococcin production. Hyicin 3682, a bacteriocin produced by one such strain, inhibited almost all strains tested, including Bacillus cereus, Listeria monocytogenes and St ... | 2011 | 22019494 |
| genotypic characterization of listeria monocytogenes isolated from humans in india. | listeria monocytogenes is a foodborne pathogen associated with severe diseases in humans and animals. the genotypic analysis of 17 l. monocytogenes isolates recovered from humans in india during 2006-2009 using multiplex serotyping pcr allowing serovar predictions, conventional serology and by pulsed field gel electrophoresis (pfge) is presented. the isolates were recovered from patients exhibiting various clinical conditions. a multiplex-pcr based serotyping assay revealed 88·24% (15/17) of the ... | 2011 | 21929876 |
| Profiling of recovery efficiencies for three standard protocols (FDA-BAM, ISO-11290, and modified USDA) on temperature-injured Listeria monocytogenes. | There have been a number of studies conducted in order to compare the efficiencies of recovery rates, utilizing different protocols, for the isolation of L. monocytogenes. However, the severity of multiple cell injury has not been included in these studies. In the current study, L. monocytogenes ATCC 19112 was injured by exposure to extreme temperatures (60°C and -20°C) for a one-step injury, and for a two-step injury the cells were transferred directly from a heat treatment to frozen state to i ... | 2011 | 21952372 |
| Temporal regulation of rapamycin on memory CTL programming by IL-12. | Mammalian target of rapamycin (mTOR) is a master regulator of cell growth. Recent reports have defined its important role in memory cytotoxic T lymphocyte (CTL) differentiation in infections and memory programming. We report that rapamycin regulated memory CTL programming by IL-12 to a similar level in a wide range of concentrations, and the enhanced memory CTLs by rapamycin were functional and provided similar protection against Listeria Monocytogenes challenge compared to the control. In addit ... | 2011 | 21966447 |
| Molecular Techniques in Ecohealth Research Toolkit: Facilitating Estimation of Aggregate Gastroenteritis Burden in an Irrigated Periurban Landscape. | Assessment of microbial hazards associated with certain environmental matrices, livelihood strategies, and food handling practices are constrained by time-consuming conventional microbiological techniques that lead to health risk assessments of narrow geographic or time scope, often targeting very few pathogens. Health risk assessment based on one or few indicator organisms underestimates true disease burden due a number of coexisting causative pathogens. Here, we employed molecular techniques i ... | 2011 | 22146856 |
| listeria peritonitis in patients on peritoneal dialysis: two cases and a review of the literature. | two cases are reported of patients on continuous ambulatory peritoneal dialysis who presented with peritonitis caused by listeria monocytogenes. they were successfully treated with intraperitoneal and intravenous administration of amoxicillin. in patients on peritoneal dialysis, listeria monocytogenes is a very rare cause of peritonitis, with only 11 cases reported to date, and mainly occurring in immunocompromised patients. in contrast to the majority of the reported cases, neither of our patie ... | 2011 | 22058269 |
| first-line treatment with cephalosporins in spontaneous bacterial peritonitis provides poor antibiotic coverage. | abstract objective. spontaneous bacterial peritonitis is a common infection in cirrhosis, associated with a high mortality. third-generation cephalosporins are recommended as first-line treatment. the aim was to evaluate the epidemiology of microbiological ascitic fluid findings and antimicrobial resistance in denmark. material and methods. all patients with cirrhosis and a positive ascitic fluid culture, at three university hospitals in the copenhagen area during a 7-year period, were re ... | 2011 | 22191479 |
| development of a listeria monocytogenes-based vaccine against hepatocellular carcinoma. | live attenuated listeria monocytogenes (lm) is a promising bacterial vector able to induce a t-cell response to tumor-associated antigens and demonstrates great potential for use in vaccine development. a novel recombinant lm-based vaccine (lmdd (lm δdalδdat)-mpfg (multiple peptide fusing genes)) was developed with the ability to express and secrete hepatocellular carcinoma (hcc)-related tumor-associated antigens fragments due to the insertion of hepatitis b virus (hbv)-x protein (hbx)-derived e ... | 2011 | 21927025 |
| listeria monocytogenes infection of the alimentary tract (enteric listeriosis) of sheep in new zealand. | a retrospective study of the microscopical lesions of nine cases of enteric listeriosis of sheep was conducted. lesions were present variably in the abomasum and the small and large intestines. the inflammation was multifocal to extensive, mainly neutrophilic and involved the lamina propria, muscularis mucosa and superficial submucosa, with intense focus on the muscularis mucosa. the mesenteric lymph nodes were also affected and, in some sheep, the liver. large numbers of gram-positive rods were ... | 2011 | 21925677 |
| role of quantitative risk assessment and food safety objectives in managing listeria monocytogenes on ready-to-eat meats. | listeria monocytogenes may be found on ready-to-eat (rte) meats, posing a public health risk. to minimize the public health impact, an appropriate level of protection (alop) can be established for a population with respect to l. monocytogenes, and ideally should be based on a scientific assessment of the risk, as well as societal and economic factors. food safety systems can be based on meeting the alop. food safety objectives (fso) provide a link between the alop and performance objectives that ... | 2006 | 22062717 |
| prevalence of listeria species in camel sausages from retail markets in aydin province in turkey and rapd analysis of listeria monocytogenes isolates. | samples were taken from 100 camel sausages from the different retail markets in aydin province in the south-west of turkey and they were tested for the presence of listeria spp by biochemical methods. samples were enriched using listeria enrichment broth and they were inoculated onto listeria selective agar. listeria monocytogenes was isolated from nine samples (9%), listeria innocua from 14 samples (14%) and listeria welshimeri from two samples(2%). a 701 bp fragment of listeriolysin o sequence ... | 2006 | 21851683 |
| effect of selected dairy starter cultures on microbiological, chemical and sensory characteristics of swine and venison (dama dama) nitrite-free dry-cured sausages. | the aim of this study was the evaluation of selected lactic acid bacteria (lab) starter culture of dairy origin in the production of nitrite-free low-acid fermented venison (dama dama) sausage (salame di daino) produced in a small-scale plant in umbria (italy), and their effect on microbiological, physico-chemical and sensorial properties of the products. salame di daino was obtained with two different processes: with and without the addition of selected lab starter cultures. microbial counts of ... | 2012 | 22032919 |
| antagonistic effect on listeria monocytogenes and l. innocua of a bacteriocin-like metabolite produced by lactic acid bacteria isolated from sucuk. | two lactobacilli and four pediococci strains producing bacteriocin-like metabolities isolated from sucuk were tested with agar spot tests and well diffusion assays for their inhibitory activity against 16 listeria strains, also isolated from sucuk. the production of organic acids and hydrogen peroxide limited, l. sake lb 706 (used as a bacteriocin producer strain) and the isolated lactic acid bacteria (lab) showed inhibitory activity against all of the listeria strains, while l. sake lb 706-a (u ... | 2001 | 22062969 |
| Inactivation of Listeria monocytogenes on agar and processed meat surfaces by atmospheric pressure plasma jets. | An apparatus for generating atmospheric pressure plasma (APP) jet was used to investigate the inactivation of Listeria monocytogenes on the surface of agar plates and slices of cooked chicken breast and ham. He, N2 (both 7 L/min), and mixtures of each with O2 (0.07 L/min) were used to produce the plasma jets. After treatment for 2 min with APP jets of He, He + O2, N2, or N2 + O2, the numbers of L. monocytogenes on agar plates were reduced by 0.87, 4.19, 4.26, and 7.59 log units, respectively. Si ... | 2011 | 21925030 |
| Use of a DNA microarray for detection and identification of bacterial pathogens associated with fishery products. | We established a microarray for the simultaneous detection and identification of diverse putative pathogens often associated with fishery products by targeting specific genes of Listeria monocytogenes, Salmonella, Shigella, Staphylococcus aureus, Streptococcus pyogenes, Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, and Yersinia enterocolitica and the 16S-23S rRNA gene internal transcribed spacer (ITS) region of Proteus mirabilis and Proteus vulgaris. The microarray contained 26 sp ... | 2011 | 21965411 |
| Contemporary unconventional clinical use of co-trimoxazole. | Clin Microbiol Infect 2012; 18: 8-17 ABSTRACT: In the late 1960s, the combination of trimethoprim and sulphamethoxazole (co-trimoxazole) was introduced into clinical practice and used to treat many infectious diseases, such as urinary tract infections, respiratory infections, sexually transmitted diseases, Gram-negative sepsis, enteric infections and typhoid fever. Subsequently, co-trimoxazole was reported to be effective against numerous bacterial, fungal and protozoal pathogens, including Noca ... | 2012 | 21851485 |
| Initial adhesion of Listeria monocytogenes to solid surfaces under liquid flow. | Some strains of the food borne pathogen Listeria monocytogenes persist in food processing environments. The exact reason behind this phenomenon is not known, but strain differences in the ability to adhere to solid surfaces could offer an explanation. In the present work, initial adhesion of nine strains of L. monocytogenes was investigated under liquid flow at two levels of shear stress on six different surfaces using a flow chamber set-up with microscopy measurements. The surfaces tested were ... | 2012 | 21968113 |
| production of bacteriocin-like metabolites by lactic acid cultures isolated from sucuk samples. | a total of 51 sucuk samples, obtained from different regions of turkey, were examined for lactic acid bacteria (lab) strains producing putative bacteriocin-like metabolites. for detection of antagonistic activity, agar spot and well diffusion assay tests were used. lactobacillus sake lb790, listeria monocytogenes li6, staphylococcus aureus st44 and escherichia coli nrrl b-3704 were used as indicator organisms. strains having antimicrobial activity were also tested against clostridium perfringens ... | 2000 | 22060908 |
| antibiotic desensitization therapy in secondary syphilis and listeria infection: case reports and review of desensitization therapy. | two adult cases, one of secondary syphilis and one of listeria monocytogenes bacteremia, in which antibiotic desensitization therapy was utilized to assist treatment of active infection in the face of severe penicillin allergy. clinical considerations are discussed that led to the decision to employ a formal desensitization procedure. antibiotic desensitization protocols can facilitate optimal and safe antibiotic therapy in the appropriate clinical setting. | 2011 | 22187514 |
| Influence of prior growth conditions, pressure treatment parameters, and recovery conditions on the inactivation and recovery of Listeria monocytogenes, Escherichia coli, and Salmonella Typhimurium in turkey meat. | The relatively high prevalence of Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella enterica serovar Typhimurium in various food products is of great concern to the food industry. The objective of this study was to determine the pressure-inactivation of the pathogens in a representative food model as affected by prior growth temperature, physiological age of the culture, pressure level and treatment temperature. The effect of post-treatment conditions (incubation temperature and g ... | 2011 | 22169000 |
| disruption of microtubule network rescues aberrant actin comets in dynamin2-depleted cells. | a large gtpase dynamin, which is required for endocytic vesicle formation, regulates the actin cytoskeleton through its interaction with cortactin. dynamin2 mutants impair the formation of actin comets, which are induced by listeria monocytogenes or phosphatidylinositol-4-phosphate 5-kinase. however, the role of dynamin2 in the regulation of the actin comet is still unclear. here we show that aberrant actin comets in dynamin2-depleted cells were rescued by disrupting of microtubule networks. dep ... | 2011 | 22174845 |
| strain dependent expression of stress response and virulence genes of listeria monocytogenes in meat juices as determined by microarray. | a subgenomic array, encompassing 54 probes targeting genes responsible for virulence, adhesion and stress response in listeria monocytogenes, was used in order to study their expression in food systems. rna extracted from l. monocytogenes inoculated in bhi and in situ (i.e. in minced meat and fermented sausage juices) and incubated at 4°c, was hybridized on the array and the results obtained were compared in order to understand the effect that the food juice has on the expression. three differen ... | 2012 | 21924790 |
| Association of ActA to peptidoglycan revealed by cell wall proteomics of intracellular Listeria monocytogenes. | Listeria monocytogenes is a Gram-positive intracellular bacterial pathogen that colonizes the cytosol of eukaryotic cells. Recent transcriptomic studies have revealed that intracellular L. monocytogenes alter expression of genes encoding envelope components. However, no comparative global analysis of this cell wall remodeling process is yet known at the protein level. Here, we used high resolution mass spectrometry to define the cell wall proteome of L. monocytogenes growing inside epithelial ce ... | 2011 | 21846725 |
| characterization and expression of attacin, an antibacterial protein-encoding gene, from the beet armyworm, spodoptera exigua (hübner) (insecta: lepidoptera: noctuidae). | to isolate antimicrobial-related genes from the beet armyworm, spodoptera exigua, we performed genefishing, a polymerase chain reaction (pcr)-based differential display technique. an attacin-like complementary dna (cdna) including a 3'-untranslated region was identified from among 18 over-expressed genes in microbial-infected larvae. the full-length attacin cdna from s. exigua cdna (seattacin) was cloned using rapid amplification of cdna ends pcr. the attacin-like cdna transcript was 765 nucleot ... | 2011 | 22160467 |
| effect of pork lard content on the chemical, microbiological and sensory properties of a typical fermented meat product (pitina) obtained from alpagota sheep. | the aim was to investigate the physicochemical, microbiological and sensory properties of pitina, a typical fermented meat product and evaluate the effect of two levels of pork lard content (low fat, lf, 10% vs. high fat, hf, 30%) on its attributes. hf attained lower ph than lf pitina, which reached lower water activity. lab comprised the major flora with substantial counts of micrococci, enterococci and mould and yeast. gram negative enterobacteria were recovered as coliforms and faecal colifor ... | 2008 | 22063596 |
| Effect of high pressure, in combination with antilisterial agents, on the growth of Listeria monocytogenes during extended storage of cooked chicken. | A cocktail of Listeria monocytogenes strains was inoculated into cooked chicken (~2.2 × 10³ CFU g?¹) which was then pressure-treated (600 MPa/2 min/20 °C) and stored for up to 105 days at 8 °C. In addition, sodium lactate (2% w/w) or a pressure-resistant Weissella viridescens strain, known to have antilisterial activity, were added to the meat prior to inoculation with the pathogen and pressure treatment, to investigate the effect on Listeria survival. Pressure treatment alone was not sufficient ... | 2011 | 21925036 |
| listeria monocytogenes mutants with altered growth phenotypes at refrigerated temperature and high salt environment. | listeria monocytogenes can survive and grow in refrigerated temperatures and high salt environment. in an effort to better understand the associated mechanisms, a library of ∼ 5200 transposon mutants of ls411, a food isolate from the jalisco cheese outbreak, were screened for their ability to grow in brain heart infusion broth at 5°c or in the presence of 7% nacl and two mutants with altered growth profiles were identified. the ls522 mutant has a transposon insertion between seca2 and iap and sh ... | 2011 | 22179239 |
| Characterization of an anti-listerial enterocin from wheat silage based Enterococcus faecium. | Two Enterococcus faecium and one E. faecalis strains isolated and identified from wheat silage were characterized based on plasmid content, hemolytic activity, antibiotic resistance patterns, bacteriocin production potential, and presence of enterocin structural genes (entA, entB, entP, entL50B). Among the isolates, only the E. faecium U7 strain exhibited bacteriocin activity against Listeria monocytogenes ATCC 7644, and vancomycin resistant Enterococcus spp. (VRE). A combination of three struct ... | 2011 | 22144257 |
| the effects of sodium lactate and starter cultures on ph, lactic acid bacteria, listeria monocytogenes and salmonella spp. levels in pure chicken dry fermented sausage. | two starter cultures (a and b) and seven sodium lactate concentrations were evaluated for chicken raw dry-fermented sausage processing. starter culture b contained more lactic acid bacteria and less staphylococci than starter a. their effects on acidification and inhibition of pathogens (listeria monocytogenes and salmonella spp.) were monitored. starter culture b grew faster and was less inhibited by sodium lactate, thus inducting a faster and more important ph drop into the sausages. with lowe ... | 2003 | 22063699 |
| Rapid detection of food- and waterborne bacteria using surface-enhanced Raman spectroscopy coupled with silver nanosubstrates. | Development of rapid and sensitive methods to detect pathogens is important to food and water safety. This study aimed to detect and discriminate important food- and waterborne bacteria (i.e., Escherichia coli O157:H7, Staphylococcus epidermidis, Listeria monocytogenes, and Enterococcus faecelis) by surface-enhanced Raman spectroscopy (SERS) coupled with intracellular nanosilver as SERS substrates. An in vivo molecular probing using intracellular nanosilver for the preparation of bacterial sampl ... | 2011 | 22005743 |
| a live-attenuated listeria vaccine (anz-100) and a live-attenuated listeria vaccine expressing mesothelin (crs-207) for advanced cancers: phase i studies of safety and immune induction. | purpose: listeria monocytogenes (lm)-based vaccines stimulate both innate and adaptive immunity. anz-100 is a live-attenuated lm strain (lm δacta/δinlb). uptake by phagocytes in the liver results in local inflammatory responses and activation and recruitment of natural killer (nk) and t cells, in association with increased survival of mice bearing hepatic metastases. the lm δacta/δinlb strain, engineered to express human mesothelin (crs-207), a tumor-associated antigen expressed by a variety of ... | 2011 | 22147941 |
| an unstructured 5'-coding region of the prfa mrna is required for efficient translation. | expression of virulence factors in the human bacterial pathogen listeria monocytogenes is almost exclusively regulated by the transcriptional activator prfa. the translation of prfa is controlled by a thermosensor located in the 5'-untranslated rna (utr), and is high at 37°c and low at temperatures <30°c. in order to develop a thermoregulated translational expression system, the 5'-utr and different lengths of the prfa-coding sequences were placed in front of lacz. when expressed in escherichia ... | 2011 | 22053088 |
| enhancing the antilisterial effect of lactobacillus curvatus cwbi-b28 in pork meat and cocultures by limiting bacteriocin degradation. | this work focused on listeria monocytogenes growth inhibition and growth rebound in raw and cooked pork meat inoculated with lactobacillus curvatus strains. during storage of raw meat homogenates in the presence of the bacteriocin-producing strain lactobacillus curvatus cwbi-b28wt, the listeria monocytogenes cfu count was initially reduced to an undetectable level, but a growth rebound occurred after two weeks, coinciding with loss of 70% of the bacteriocin activity present at the end of week 2. ... | 2008 | 22063576 |