| a novel link between campylobacter jejuni bacteriophage defence, virulence and guillain-barré syndrome. | guillain-barré syndrome (gbs) is a post-infectious disease in which the human peripheral nervous system is affected after infection by specific pathogenic bacteria, including campylobacter jejuni. gbs is suggested to be provoked by molecular mimicry between sialylated lipooligosaccharide (los) structures on the cell envelope of these bacteria and ganglioside epitopes on the human peripheral nerves, resulting in autoimmune-driven nerve destruction. earlier, the c. jejuni sialyltransferase (cst-ii ... | 2013 | 22945471 |
| comparison of different sampling strategies and laboratory methods for the detection of c. jejuni and c. coli from broiler flocks at primary production. | the objective of the study was to evaluate the performance of different combinations of sample type, transport medium and culture methods for the recovery of campylobacter jejuni and c. coli from broiler flocks at primary production. boot swabs moistened with one of four different transport media [maximum recovery diluent (n=120), exeter broth (ex) (n=120), buffered peptone water (n=120) and modified semi-solid cary-blair (n=120)], caecal samples (n=40) and faecal samples (n=120) from 40 broiler ... | 2013 | 22931208 |
| sodium metasilicate affects growth of campylobacter jejuni in fresh, boneless, uncooked chicken breast fillets stored at 4 degrees celsius for 7 days. | the objectives of this study were to determine the antimicrobial effects of sodium metasilicate (sms) treatments against campylobacter jejuni in fresh, boneless, uncooked chicken breast fillets and to ascertain the effects of sms treatments on ph. the fillets were inoculated with c. jejuni, treated with 0% sms and no inoculum (negative control), 0% sms and inoculum (positive control), 1 and 2% sms solutions, and stored at 4 ± 1°c. all samples were analyzed after 0, 1, 3, 5, and 7 d storage for c ... | 2012 | 22912470 |
| garlic derivative propyl propane thiosulfonate is effective against broiler enteropathogens in vivo. | two experiments were carried out to study the effects of dietary supplementation with the garlic (allium sativum)-derived product propyl propane thiosulfonate (pts-o) on the intestinal log(10) number of copies of enteropathogens in broiler chickens, together with their intestinal morphology and growth performance. the additive had no significant effect on feed intake at any dose assayed. in experiment 1 (1 to 21 d of age), the bw of chickens fed on 45 mg of pts-o/kg of diet was higher (p < 0.01) ... | 2012 | 22912448 |
| [basic research for the control of campylobacter food poisoning]. | appropriate handling and controlled temperature prevent cross-contamination and proliferation of contaminants in foods, thereby reducing the incidences of food-borne gastroenteritis in japan. however, the incidence of campylobacter jejuni/coli infection did not markedly decrease and has become one of the major causes of food-borne diseases. c. jejuni and c. coli are widespread in warm-blooded domestic animals; therefore, food products may easily become contaminated during processing. c. jejuni a ... | 2012 | 22894062 |
| influence of asellus aquaticus on escherichia coli, klebsiella pneumoniae, campylobacter jejuni and naturally occurring heterotrophic bacteria in drinking water. | water lice, asellus aquaticus (isopoda), frequently occur in drinking water distribution systems where they are a nuisance to consumers and water utilities. whether they are solely an aesthetic problem or also affect the microbial water quality is a matter of interest. we studied the influence of a. aquaticus on microbial water quality in non-chlorinated drinking water in controlled laboratory experiments. pure cultures of the indicator organisms escherichia coli and klebsiella pneumoniae and th ... | 2012 | 22884244 |
| farm and environmental distribution of campylobacter and salmonella in broiler flocks. | the objective of this study was to determine the prevalence of campylobacter and salmonella in broilers and their distribution in the indoor and outdoor farm environment. nine hundred samples (400 faecal; 300 indoor environment; 200 outdoor environment), were collected from 10 individual broiler houses on 10 farms. campylobacter jejuni prevalence was significantly higher (p=0.003) in faeces (29.5%; 118/400) than the environment (0.8%; 4/500) in contrast to salmonella typhimurium from faecal (8.8 ... | 2013 | 22884004 |
| anti-gq1b-negative miller fisher syndrome after campylobacter jejuni enteritis. | miller fisher syndrome is a clinical variant of guillain-barré syndrome, characterized by acute-onset ophthalmoplegia, ataxia, and areflexia. it results from an immune response to a cross-reactive antigen between gq1b ganglioside in human neurons and lipo-oligosaccharides of certain bacteria, e.g., campylobacter jejuni. anti-gq1b antibody is a powerful diagnostic marker for miller fisher syndrome. however, only a small number of anti-gq1b-negative miller fisher syndrome cases are documented. a 1 ... | 2012 | 22883289 |
| effect of crust freezing applied alone and in combination with ultraviolet light on the survival of campylobacter on raw chicken. | the application of crust freezing (cf) applied as a stand-alone treatment or in combination with ultraviolet (uv) light for reducing the level of artificially inoculated campylobacter jejuni on raw chicken was investigated. cf air temperatures of -5, -15 and -27 °c (±3 °c) with freezing times of 70, 15 and 6 min, respectively, were used. the level of c. jejuni on chicken was also examined following subsequent refrigerated (0-4 °c) storage at 3 and 7 days. all cf treatments resulted in significan ... | 2012 | 22850386 |
| purification and characterization of a new bacteriocin active against campylobacter produced by lactobacillus salivarius smxd51. | strain smxd51, isolated from chicken ceca and identified as lactobacillus salivarius, produced a component that inhibits the growth of gram-positive and gram-negative bacteria and especially campylobacter jejuni. the active peptide from the cell-free supernatant of lb. salivarius smxd51 was purified in three steps: (i) precipitation with 80% saturated ammonium sulfate, (ii) elution on a reversed phase spe upti-clean cartridge using different concentrations of acetonitrile, (iii) final purificati ... | 2012 | 22850384 |
| tracing campylobacter jejuni strains along the poultry meat production chain from farm to retail by pulsed-field gel electrophoresis, and the antimicrobial resistance of isolates. | in this study campylobacter jejuni isolates were recovered from birds, carcasses and carcass portions from two broiler chicken flocks and from equipment used for carcass and meat processing along the production chain from farms to retail stores. isolates were subjected to pulsed-field gel electrophoresis (pfge) using smai and kpni restriction enzymes and their antimicrobial susceptibilities were determined. c. jejuni was recovered from product and equipment used with both flocks at each point in ... | 2012 | 22850383 |
| behaviour of non-stressed and stressed listeria monocytogenes and campylobacter jejuni cells on fresh chicken burger meat packaged under modified atmosphere and inoculated with protective culture. | numerous investigations have provided evidence that chicken products are a source of listeria monocytogenes and campylobacter jejuni. different strategies applied in final products are needed to prevent consumers' contamination. in this work, the combination of modified atmosphere packaging (map) and protective culture to control the growth of freeze stressed and non-stressed l. monocytogenes and c. jejuni on fresh chicken meat burger was studied. meat burgers were inoculated with l. monocytogen ... | 2012 | 22831819 |
| multilocus sequence types of environmental campylobacter jejuni isolates and their similarities to those of human, poultry and bovine c. jejuni isolates. | in this study, we investigated the multilocus sequence type (mlst) diversity and population genetics of campylobacter jejuni isolates collected from the natural waters (n = 57), wild birds (n = 37) and zoo animals (n = 19) in southern finland, the helsinki area and the helsinki zoo, respectively. on average, we found c. jejuni in 20%, 10.4% or 11.5% of the samples collected from natural waters, wild birds and zoo animals, respectively. high st diversity was detected in all three sources and 41.2 ... | 2013 | 22827634 |
| reduced spread of campylobacter jejuni in broiler chickens by stimulating the bird's natural barriers. | we have tested the effect of feed structure and feeding regime to prevent the spread of the zoonotic pathogen campylobacter jejuni in broiler chicken flocks. | 2012 | 22817452 |
| distribution and genotypic characterization of campylobacter jejuni isolated from poultry in split and dalmatia county, croatia. | consumption of poultry contaminated with campylobacter jejuni has been recognized worldwide as the leading cause of campylobacteriosis. therefore, the aim of our study was to investigate the prevalence and genotype diversity of campylobacter jejuni in poultry meat intended for consumption in split and dalmatia county, which is the second biggest county in croatia. furthermore, we also wanted to discover possibly stable clones of c. jejuni appearing in different samples and periods of time, which ... | 2013 | 22805333 |
| evaluating the efficacy of an avian-specific probiotic to reduce the colonization of campylobacter jejuni in broiler chickens. | campylobacteriosis is the most frequent zoonotic disease in humans worldwide, and the contaminated poultry meat by campylobacter jejuni can be considered one of the important sources of enteric infections in humans. the use of probiotics, which can help to improve the natural defense of animals against pathogenic bacteria, is an alternative and effective approach to antibiotic administration for livestock to reduce bacterial contamination. in vitro experiments showed that enterococcus faecium, p ... | 2012 | 22802174 |
| benign ulcer of the right colon clinically misdiagnosed as carcinoma: an additional case. | benign solitary ulcer of the colon is an uncommon lesion that was originally described by cruveilhier in 1832. its aetiology remains unknown, and there are no pathognomonic lesions or symptoms. diagnosis is made by exclusion; in fact, diseases such as specific infections (cytomegalovirus, campylobacter jejuni, entamoeba histolytica), common clinical conditions (acute appendicitis, diverticulitis, intestinal obstruction, inflammatory bowel disease), pharmacotherapy (non-steroidal anti-inflammator ... | 2012 | 22799054 |
| cloning, expression, and antigenicity of 14 proteins from campylobacter jejuni. | fourteen campylobacter jejuni genes--pora, cadf, omp18, dnak, flac, peb1, peb2, peb3, peb4, ahpc, groel, tuf, hipo, and cj0069--were cloned and expressed in escherichia coli bl21. the recombinant proteins were purified on histidine (his) and glutathione s-transferase (gst) trap columns using the äkta explorer 100 system. recombinant proteins were visualized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified using matrix-assisted laser desorption ionization time-of-fli ... | 2012 | 22779748 |
| real-time taqman polymerase chain reaction-based genus-identification and pyrosequencing-based species identification of campylobacter jejuni, c. coli, c. lari, c. upsaliensis, and c. fetus directly on stool samples. | a new method was developed for campylobacter identification and applied directly on 599 stool samples from diarrhoeagenic patients. here, the gyrase b gene of campylobacter was targeted in a 2-step process: first, taqman polymerase chain reaction (pcr)-based identification of c. jejuni, c. coli, c. upsaliensis, c. lari, and c. fetus at the genus level, and, second, pyrosequencing-based identification at the species level. the taqman pcr method was compared to culturing and identified 87 campylob ... | 2012 | 22770773 |
| carbohydrate binding and gene expression by in vitro and in vivo propagated campylobacter jejuni after immunomagnetic separation. | campylobacter jejuni is an important human food-borne intestinal pathogen, however relatively little is known about its mechanisms of pathogenesis or pathogen-host interactions. to monitor changes in gene expression and glycan binding of c. jejuni within a common avian host, an immunomagnetic separation technique (ims) was utilised to directly isolate infecting c. jejuni 81116 from a chicken host. an average of 10(5) cells/g was re-isolated from chicken caecal samples by ims technique. the in vi ... | 2013 | 22753110 |
| survival of campylobacter jejuni in chicken meat at frozen storage temperatures. | the aim of this study was to determine the survival of campylobacter jejuni in chicken meat samples at frozen temperatures and given length of incubation and to determine the impact of aerobic bacteria on the survival of c. jejuni. the chicken meat samples were inoculated with c. jejuni nctc 11351 suspensions and stored in bags at temperatures of -20°c and -70°c. the mean value of c. jejuni from meat samples decreased from 7.52 log10 cfu/g after 30 minutes of incubation at ambient temperature, t ... | 2012 | 22750779 |
| long-amplicon propidium monoazide-pcr enumeration assay to detect viable campylobacter and salmonella. | the effect of amplicon length on the ability of propidium monoazide-pcr (pma-pcr) to reliably quantify viable cells without interference from dead cells was tested on heat- and ultraviolet (uv)-killed salmonella enterica and campylobacter jejuni, two important enteric pathogens of concern in environmental, food and clinical samples. | 2012 | 22747901 |
| phenotypic and genotypic characterizations of campylobacter jejuni isolated from the broiler meat production process. | a set of c. jejuni isolates of different origins and flaa-genotypes obtained throughout the broiler meat production chain was tested in this study for a possible correlation of their origin, phylogenetic relationship, and phenotypic properties. interestingly, the results showed a correlation of the origin and the phylogenetic relationship between the c. jejuni isolates and their ability to form biofilm, but not in their ability to survive at -18, 5, 20, and 48 °c. two strains, a broiler cloacae ... | 2012 | 22735984 |
| a case of myopericarditis associated to campylobacter jejuni infection in the southern hemisphere. | myopericarditis is an infrequent complication of acute diarrheal illness due to campylobacter jejuni, and it has been mainly reported in developed nations. the first case detected in chile--an upper-middle income country--that is coincidental with the increasing importance of acute gastroenteritis associated to this pathogen, is described. recognition of this agent in stools requires special laboratory techniques not widely available, and it was suspected when a young patient presented with acut ... | 2017 | 22729200 |
| campylobacter jejuni is highly susceptible to killing by chicken host defense peptide cathelicidin-2 and suppresses intestinal cathelicidin-2 expression in young broilers. | little is known about the interactions of chicken host defense peptides (hdps) with campylobacter jejuni in young chicks. to examine the role of the chicken hdp, cathelicidin-2 (cath-2) in host-pathogen interactions we challenged 4-day-old ross 308 broilers with a chicken-derived c. jejuni isolate (ws356) and used the chicken pathogen salmonella enterica enteritidis phage type 4 (fgt1) as a reference. immunohistochemical staining was used to localize cath-2, c. jejuni and salmonella enteritidis. ... | 2012 | 22728124 |
| the gut microbiota elicits a profound metabolic reorientation in the mouse jejunal mucosa during conventionalisation. | proper interactions between the intestinal mucosa, gut microbiota and nutrient flow are required to establish homoeostasis of the host. since the proximal part of the small intestine is the first region where these interactions occur, and since most of the nutrient absorption occurs in the jejunum, it is important to understand the dynamics of metabolic responses of the mucosa in this intestinal region. | 2013 | 22722618 |
| application of medium-chain fatty acids in drinking water increases campylobacter jejuni colonization threshold in broiler chicks. | campylobacteriosis is the most reported bacterial-mediated gastroenteritic disease in many developed countries. broiler chickens are a natural host for campylobacter spp., and contaminated poultry meat products are a major source for transmitting pathogenic campylobacter strains to humans. currently, no intervention measure efficiently and effectively controls this pathogen in poultry flocks. medium-chain fatty acids (caproic, caprylic, capric, and lauric acids) show a marked anti-campylobacter ... | 2012 | 22700521 |
| survival of campylobacter jejuni in chicken meat, chicken skin and chicken liver at low temperatures. | the aim of this study was to determine survival of campylobacter jejuni (c. jejuni) in chicken meat, chicken skin medallions and chicken liver contaminated with this bacterium at +4 °c and -20 °c, after 24 hours of incubation. the survival of c. jejuni at +4 °c, -20 °c in chicken meat, chicken skin medallions and chicken liver were examined after 24 hours of incubation with c. jejuni. all samples were previously tested for the presence of campylobacter species according to iso 10272-1:2006. afte ... | 2012 | 22693971 |
| thermal inactivation of campylobacter jejuni in broth. | new zealand has a high rate of reported campylobacteriosis compared with other developed countries. one possible reason is that local strains have greater heat tolerance and thus are better able to survive undercooking; this hypothesis is supported by the remarkably high d-values reported for campylobacter jejuni in the netherlands. the objective of this study was to investigate the thermal inactivation of isolates from new zealand in broth, using strains that are commonly found in human cases a ... | 2012 | 22691469 |
| identifying and testing candidate genetic polymorphisms in the irritable bowel syndrome (ibs): association with tnfsf15 and tnfα. | the postinfectious irritable bowel syndrome (pi-ibs) suggests that impaired resolution of inflammation could cause ibs symptoms. the authors hypothesised that polymorphisms in genes whose expression were altered by gastroenteritis might be linked to ibs with diarrhoea (ibs-d) which closely resembles pi-ibs. | 2013 | 22684480 |
| anti-infective bovine colostrum oligosaccharides: campylobacter jejuni as a case study. | campylobacter jejuni is the leading cause of acute bacterial infectious diarrhea in humans. unlike in humans, c. jejuni is a commensal within the avian host. heavily colonized chickens often fail to display intestinal disease, and no cellular attachment or invasion has been demonstrated in-vivo. recently, researchers have shown that the reason for the attenuation of c. jejuni virulence may be attributed to the presence of chicken intestinal mucus and more specifically chicken mucin. since mucins ... | 2012 | 22647676 |
| campylobacter spp., c. jejuni and c. upsaliensis infection-associated factors in healthy and ill dogs from clinics in cordoba, spain. screening tests for antimicrobial susceptibility. | a microbiological and epidemiological survey examining campylobacter species-related infection in dogs from clinics in cordoba (southern spain) was performed from may 2007 to march 2008. three-hundred and six rectal swabs were taken from both ill and healthy dogs, and an epidemiological questionnaire was filled out. the isolated bacterial strains were identified by means of multiplex polymerase chain reaction (pcr). the prevalence factors for campylobacter spp., c. jejuni and c. upsaliensis infe ... | 2012 | 22640550 |
| antimicrobial resistance of emerging foodborne pathogens: status quo and global trends. | emerging foodborne pathogens are challenging subjects of food microbiology with their antibiotic resistance and their impact on public health. campylobacter jejuni, salmonella spp. and verotoxigenic escherichia coli (vtec) are significant emerging food pathogens, globally. the decrease in supply and increase in demand lead developed countries to produce animal products with a higher efficiency. the massive production has caused the increase of the significant foodborne diseases. the strict contr ... | 2013 | 22639875 |
| effect of temperature and antimicrobial resistance on survival of campylobacter jejuni in well water: application of the weibull model. | the aims of this study were to measure the survival of two campylobacter jejuni strains and their in vitro-challenged antimicrobial-resistant variants in well water, to evaluate the effects of antimicrobial resistance on survival and to develop a mathematical model for predicting the survival of camp. jejuni in well water in the temperature range from 4 to 25°c. | 2012 | 22612521 |
| importance of rna stabilization: evaluation of ansb, ggt, and rpoa transcripts in microaerophilic campylobacter jejuni 81-176. | a subset of food-borne campylobacter jejuni strains utilizes amino acids asparagine and glutamine as carbon sources that may enhance the ability of this microaerophilic pathogen to colonize specific tissues. in this study, we analyzed the transcript sizes of the ansb and ggt genes encoding the periplasmic asparaginase and γ-glutamyltranspeptidase in c. jejuni 81-176, respectively, and compared the expression level of mrnas at different time points during the growth in vitro. in addition, we incl ... | 2012 | 22610497 |
| a bifidobacterium-based synbiotic product to reduce the transmission of c. jejuni along the poultry food chain. | with the ban of dietary antimicrobial agents, the use of probiotics, prebiotics and synbiotics has attracted a great deal of attention in order to improve intestinal health and control food-borne pathogens, which is an important concern for the production of safe meat and meat products. recently, campylobacter jejuni has emerged as a leading bacterial cause of food-borne gastroenteritis in humans, and epidemiological evidences indicate poultry and poultry products as the main source of human inf ... | 2012 | 22608658 |
| characterization of two putative mechanosensitive channel proteins of campylobacter jejuni involved in protection against osmotic downshock. | acute hypotonic stress becomes a threat to the survival of bacteria in the environment. mechanosensitive channels play an essential role in the maintenance of bacterial cell integrity during hypoosmotic shock. a database search suggested that campylobacter jejuni, a major worldwide cause of bacterial gastroenteritis in humans, possesses two putative mechanosensitive channels, designated cjj0263 and cjj1025, in c. jejuni strain 81-176. osmotic downshock experiments demonstrated that a mutant lack ... | 2012 | 22608101 |
| campylobacter jejuni cutaneous infection in a patient with graft versus host disease. | | 2012 | 22595471 |
| [development and application of taqman mgb probe fluorescence quantitative pcr method for rapid detection of clostridium piliforme]. | to develop a taqman mgb probe-based, sensitive and specific fluorescence quantitative pcr assay method for rapid detection of clostridium piliforme. | 2012 | 22575149 |
| barriers to horizontal gene transfer in campylobacter jejuni. | campylobacter jejuni is among the most frequent agent of foodborne gastroenteritis in the world, but its physiology and pathogenesis is less well understood than other bacterial enteric pathogens. this is due in part to the incompatibility of the molecular tools that have enabled advances in the characterization of other bacterial species. most notably, the dearth of plasmid-based complementation, reporter assays, and plasmid-based unmarked mutagenesis procedures in many of the type strains has ... | 2012 | 22569516 |
| an inactivation kinetics model for campylobacter jejuni on chicken meat under low-temperature storage. | campylobacter jejuni is susceptible to low temperatures. freezing and chilling are effective interventions for reducing the occurrence of c. jejuni on poultry meat. the survival rates of three c. jejuni strains (atcc33560, jr0706-2, and alm-80) inoculated onto chicken meat samples were measured at -20°c and 4°c, and the survival curves of these three strains were determined. the results showed that the number of surviving cells decreased by 3.16, 2.87, and 3.14 log colony-forming unit (cfu)/g, r ... | 2012 | 22568749 |
| a polymer microfluidic chip for quantitative detection of multiple water- and foodborne pathogens using real-time fluorogenic loop-mediated isothermal amplification. | inexpensive, portable, and easy-to-use devices for rapid detection of microbial pathogens are needed to ensure safety of water and food. in this study, a disposable polymer microfluidic chip for quantitative detection of multiple pathogens using isothermal nucleic acid amplification was developed. the chip contains an array of 15 interconnected reaction wells with dehydrated primers for loop-mediated isothermal amplification (lamp), and requires only a single pipetting step for dispensing of sam ... | 2012 | 22566273 |
| [campylobacter infections in children]. | campylobacter infections are essentially enteric infections frequently occurring before 15 years of age. the main species responsible for these infections is campylobacter jejuni. the infection is observed mainly during summertime, and boys are more often affected than girls. the transmission is usually food-borne (poultry or cross-contamination of raw food). environmental contamination is also possible. in addition to the digestive symptoms, systemic infectious complications or postinfectious c ... | 2012 | 22559950 |
| prospective study of human norovirus infection in children with acute gastroenteritis in greece. | noroviruses are considered as a major cause of acute gastroenteritis in childhood worldwide. this prospective study was undertaken to investigate the frequency and clinical features of norovirus infections in children aged less than 5 years with acute gastroenteritis in greece. | 2012 | 22555327 |
| immunoproliferative small intestinal disease associated with campylobacter jejuni. | | 2012 | 22552252 |
| dynamics of populations of campylobacter jejuni in two grandparent broiler breeder farms: persistent vs. transient strains. | the objectives of the study were to characterize and investigate the populations of campylobacter jejuni in two grandparent broiler breeder farms over four years. caecal as well as farm environmental samples were obtained. campylobacter isolates were characterized by macrorestriction profile (smai and kpni-pfge) and pcr-rflp of the flaa gene. susceptibility tests against seven antimicrobials were also performed. birds were negative for campylobacter spp. when they came to these two production fa ... | 2012 | 22551591 |
| bactericidal effect of hydrolysable and condensed tannin extracts on campylobacter jejuni in vitro. | strategies are sought to reduce intestinal colonisation of food-producing animals by campylobacter jejuni, a leading bacterial cause of human foodborne illness worldwide. presently, we tested the antimicrobial activity of hydrolysable-rich blackberry, cranberry and chestnut tannin extracts and condensed tannin-rich mimosa, quebracho and sorghum tannins (each at 100 mg/ml) against c. jejuni via disc diffusion assay in the presence of supplemental casamino acids. we found that when compared to non ... | 2012 | 22528299 |
| recent developments in bacterial protein glycan coupling technology and glycoconjugate vaccine design. | the discovery of the campylobacter jejuni n-linked glycosylation system combined with its functional expression in escherichia coli marked the dawn of a new era in glycoengineering. the process, termed protein glycan coupling technology (pgct), has, in particular, been applied to the development of glycoconjugate vaccines. in this review, we highlight recent technical developments in this area, including the first structural determination of the coupling enzyme pglb, the use of glycotags for opt ... | 2012 | 22516134 |
| analysis of campylobacter jejuni whole-genome dna microarrays: significance of prophage and hypervariable regions for discriminating isolates. | campylobacter is a leading cause of foodborne illness in humans, and improving our understanding of the epidemiology of this organism is essential. the objective of this study was to identify the genes that discriminate isolates of c. jejuni by analysis with whole-genome dna microarrays. statistical analyses of whole-genome data from 95 geographically diverse cattle, chicken, and human c. jejuni isolates identified 142 most significant variable genes. of this total, 125 (88%) belonged to genomic ... | 2012 | 22506962 |
| evaluation of current molecular approaches for genotyping of campylobacter jejuni strains. | campylobacter jejuni has been recognized as the most common bacterial cause of gastroenteritis worldwide, in both developed and developing countries, since the late 1970s. a number of genotyping schemes have been developed to identify the sources and route of transmission of these foodborne pathogens so that proper control measures can be developed. in this review, we provide current genotypic schemes developed for campylobacter spp. (particularly c. jejuni) over the last decades, along with an ... | 2012 | 22506653 |
| influence of enteric bacteria, parasite infections and nutritional status on diarrhoea occurrence among 6-60 months old children admitted at a regional hospital in morogoro, tanzania. | while nutritional, microbiological and immunological factors have been implicated in childhood diarrhoea in many countries, there is limited aetiological information in morogoro region of tanzania. a case-control study was conducted to establish whether diarrhoea in 6-60 months old children admitted at a regional hospital in morogoro, was attributable to enteric bacteria and/or parasites and the contribution of under-nutrition, as measured by weight-for-age below -2 sd. from january to september ... | 2012 | 26591731 |
| an engineered eukaryotic protein glycosylation pathway in escherichia coli. | we performed bottom-up engineering of a synthetic pathway in escherichia coli for the production of eukaryotic trimannosyl chitobiose glycans and the transfer of these glycans to specific asparagine residues in target proteins. the glycan biosynthesis was enabled by four eukaryotic glycosyltransferases, including the yeast uridine diphosphate-n-acetylglucosamine transferases alg13 and alg14 and the mannosyltransferases alg1 and alg2. by including the bacterial oligosaccharyltransferase pglb from ... | 2012 | 22446837 |
| the role of wlarg, wlatb and wlatc in lipooligosaccharide synthesis by campylobacter jejuni strain 81116. | campylobacter jejuni is a major bacterial cause of gastroenteritis world-wide. c. jejuni produces a range of glycans including lipooligosaccharide (los), an important virulence factor. the genetic content of the los synthesis locus varies between c. jejuni strains and 19 classes have been described. three los synthesis genes of c. jejuni strain 81116 (nctc 11828), wlarg, wlatb and wlatc were the focus of this study. wlarg and the remaining two proteins of interest share sequence similarity to am ... | 2012 | 22445818 |
| developing an anti-campylobacter jejuni vaccine. | the proteomics era allows for the definition of biological organisms at the protein level, offering new opportunities for delimiting the self- and non-self boundaries between microbes and the human host. here, we apply proteomics to design a vaccine against the major pathogenic factor of campylobacter jejuni, i.e. cytolethal distending toxin (cdt). we used the scientific rationale that only peptide sequences not represented in the host proteome have the potential to evoke powerful, non-cross-rea ... | 2012 | 22428568 |
| gene expression profile of campylobacter jejuni-induced gbs in bama miniature pigs. | our aim was to investigate the in vivo gene expression pattern of the guillain-barre syndrome (gbs) with dna microarrays and bioinformatics tools. oral-infusion model animals mimicking human infection of gbs were analyzed. tissue samples and body fluids were collected to perform antibody tests and biopsy assays. gene-expression microarray was conducted with nerve tissues and gbs-related genes were elucidated via bioinformatics tools. model animals showed typical symptoms of gbs in that mild demy ... | 2012 | 22427118 |
| survival of campylobacter jejuni and salmonella enterica typhimurium in vacuum-packed, moisture-enhanced pork. | the abilities of campylobacter jejuni and salmonella enterica typhimurium to survive in vacuum-packaged, moisture-enhanced pork stored at 4 or 10°c were examined. pork loins were surface inoculated with either c. jejuni or salmonella typhimurium and then moisture enhanced to a target of 10 or 20%. the enhanced pork loins were sliced 1 cm thick and vacuum packaged. a pork loin without moisture enhancement was sliced and vacuum packaged as a control. samples were collected, plated, and the numbers ... | 2012 | 22410234 |
| high pressure inactivation of escherichia coli, campylobacter jejuni, and spoilage microbiota on poultry meat. | this study evaluated the high pressure inactivation of campylobacter jejuni, escherichia coli, and poultry meat spoilage organisms. all treatments were performed in aseptically prepared minced poultry meat. treatment of 19 strains of c. jejuni at 300 mpa and 30°c revealed a large variation of pressure resistance. the recovery of pressure-induced sublethally injured c. jejuni depended on the availability of iron. the addition of iron content to enumeration media was required for resuscitation of ... | 2012 | 22410223 |
| hydrogenase activity in the foodborne pathogen campylobacter jejuni depends upon a novel abc-type nickel transporter (nikzyxwv) and is slyd-independent. | campylobacter jejuni is a human pathogen of worldwide significance. it is commensal in the gut of many birds and mammals, where hydrogen is a readily available electron donor. the bacterium possesses a single membrane-bound, periplasmic-facing nife uptake hydrogenase that depends on the acquisition of environmental nickel for activity. the periplasmic binding protein cj1584 (nikz) of the atp binding cassette (abc) transporter encoded by the cj1584c-cj1580c (nikzyxwv) operon in c. jejuni strain n ... | 2012 | 22403188 |
| "ulcerative crepitus" -- a case with subcutaneous emphysema and pneumomediastinum without colonic perforation or toxic megacolon in ulcerative colitis successfully treated conservatively. | a 19-year-old man with a 1-year history of ulcerative colitis presented with fever, bloody diarrhea and severe dehidration. he was on po.48 mg methylprednisolon and 3 g mesalazine daily, and has recently finished taking chlarythromycin for campylobacter jejuni infection. on physical examination, no abdominal tenderness was found, but surprisingly, extensive bilateral subcutaneous emphysema was detected in the supraclavicular regions. laboratory tests proved anaemia, elevated white blood cell cou ... | 2012 | 22398071 |
| a waterborne outbreak with a single clone of campylobacter jejuni in the danish town of køge in may 2010. | in denmark, large-scale waterborne outbreaks are rare. this report describes the investigation of an outbreak that occurred in the town of køge in may 2010. | 2012 | 22385125 |
| effect of mannoproteins on the growth, gastrointestinal viability, and adherence to caco-2 cells of lactic acid bacteria. | yeast cell wall (ycw) preparations and yeast mannoprotein extracts have been effective against some enteropathogenic bacteria as campylobacter jejuni, escherichia coli, and salmonella, and they can affect the population of beneficial lactic acid bacteria (lab). in this work, we studied the effect of a mannoprotein extract on five strains of lab. this extract was metabolised by the bacteria, enhancing their survival in simulated gastrointestinal juice, and increasing the adherence of lactobacillu ... | 2012 | 22384965 |
| eimeria species parasites as novel vaccine delivery vectors: anti-campylobacter jejuni protective immunity induced by eimeria tenella-delivered cjaa. | vaccination of poultry against coccidiosis caused by the eimeria species is almost entirely based upon varied formulations of live parasites. the recent development of a series of protocols that support genetic complementation by transfection in eimeria now provides an opportunity to utilise live anticoccidial vaccines to deliver additional vaccinal antigens. the capacity of eimeria tenella to express an exogenous antigen and induce an immune response during in vivo infection which is protective ... | 2012 | 22342500 |
| clinical manifestations of campylobacter jejuni infection in adolescents and adults, and change in antibiotic resistance of the pathogen over the past 16 years. | campylobacter jejuni infection, traditionally a paediatric illness, is now seen more frequently in adolescents and adults in northern china. published surveillance reports on c. jejuni infection in these patients are rare. we aimed to characterize (1) the clinical manifestations of this infection in adolescents and adults, and (2) changes in antibiotic resistance of the pathogen. | 2012 | 22339578 |
| differentiation of the virulence potential of campylobacter jejuni strains by use of gene transcription analysis and a caco-2 assay. | campylobacter jejuni is the leading cause of bacterial diarrheal disease in humans, and contaminated poultry and poultry products are recognized as the main vehicle of infection. despite the significance of c. jejuni as a foodborne pathogen, little is known about its response to stress, and, especially, how its virulence is modulated under such conditions. the aim of this study was to assess the effect of temperature shift in a broth model system on virulence expression and cell survival of thre ... | 2012 | 22336513 |
| a cytolethal distending toxin gene-based multiplex pcr assay for detection of campylobacter spp. in stool specimens and comparison with culture method. | in this study, we evaluated the applicability of cytolethal distending toxin (cdt) gene-based species-specific multiplex pcr for the direct detection and identification of campylobacter jejuni, c. coli and c. fetus from stool specimens of patients with gastroenteritis in comparison to culture methods. a total of 711 stool specimens were examined for the isolation or detection of campylobacters by using skirrow's selective agar culture plates, a filtration method and the multiplex pcr assay. fort ... | 2012 | 22322187 |
| lipooligosaccharide locus classes are associated with certain campylobacter jejuni multilocus sequence types. | the lipooligosaccharide (los) locus class was determined using polymerase chain reaction (pcr) in 335 finnish campylobacter jejuni strains isolated from humans, poultry and bovines with known multilocus sequence types. the results revealed an association between clonal complexes/sequence types (sts) and los locus classes. based on these results, we further predicted the los locus classes distribution among the sts of 209 additional c. jejuni strains from finnish human domestically acquired infec ... | 2012 | 22298242 |
| guillain-barré syndrome associated with acute hepatitis e infection. | guillain-barré syndrome (gbs) is a neurological emergency that warrants prompt diagnosis and treatment. occasionally, the clinical, laboratory and electrophysiological features are neither typical nor diagnostic. it requires a high index of suspicion and clinical judgement for early diagnosis and commencement of treatment in those scenarios. some pathogens are potential triggers of this serious neurological disease, including: campylobacter jejuni, mycoplasma pneumoniae and cytomegalovirus. alth ... | 2012 | 22285113 |
| antagonistic activity of lactobacillus acidophilus atcc 4356 on the growth and adhesion/invasion characteristics of human campylobacter jejuni. | the aim of this research was to determine the potential probiotic activity of lactobacillus acidophilus atcc 4356 against several human campylobacter jejuni isolates. the ability to inhibit the pathogen's growth was evaluated by co-culture experiments as well as by antimicrobial assays with cell-free culture supernatant (cfcs), while interference with adhesion/invasion to intestinal caco-2 cells was studied by exclusion, competition, and displacement tests. in the co-culture experiments l. acido ... | 2012 | 22271268 |
| analysis of risk factors associated with antibiotic-resistant escherichia coli. | antimicrobial-resistant bacteria represent a major threat to human and animal health. we compared equine fecal samples (n=264) from 138 horses from hospital and nonhospital (livery stable and riding school) premises in north west england to determine the prevalence of escherichia coli, salmonella, and campylobacter and rates of antimicrobial-resistant e. coli strains. campylobacter jejuni was detected only in hospitalized horses (1.1%), and no salmonella was identified. data analysis of the hors ... | 2012 | 22229818 |
| campylobacter, salmonella, listeria monocytogenes, verotoxigenic escherichia coli, and escherichia coli prevalence, enumeration, and subtypes on retail chicken breasts with and without skin. | this study examined the prevalence, counts, and subtypes of campylobacter, salmonella, listeria monocytogenes, verotoxigenic escherichia coli (vtec), and e. coli on raw retail chicken breast with the skin on versus the skin off. from january to december 2007, 187 raw skin-on chicken breasts and 131 skin-off chicken breasts were collected from randomly selected retail grocery stores in the region of waterloo, ontario, canada. campylobacter isolates were recovered from a higher proportion of the s ... | 2012 | 22221353 |
| impact and management of campylobacter in human medicine--us perspective. | viruses, enteric bacteria and parasites can all produce similar syndromes of acute enteritis, although the pathophysiology and molecular pathogenesis may vary widely. the severity of acute enteritis varies greatly, and only a small fraction of cases undergo medical evaluation. there are over 200 000 000 episodes of acute enteritis annually in the usa, of which approximately 75 000 000 are foodborne. fewer than 20% of estimated cases have a known etiology. nearly half of the more than 4 000 000 c ... | 2002 | 23570172 |
| genetic differentiation of campylobacter jejuni. | genotyping methods have been developed and applied to differentiate campylobacter jejuni isolates over the last two decades. although a wealth of information was generated, the data are disappointingly complex and do not support simple models of transmission. several observations have apparently weakened the value and complicated the interpretation of genotyping methods. for several methods, instability of genotype has been demonstrated, due to recombinations with or without transformation. c. j ... | 2002 | 23570170 |
| the ecology of campylobacter jejuni in avian and human hosts and in the environment. | campylobacter jejuni, and its close relative c. coli, are highly successful bacteria colonizing the intestinal mucosa of a wide range of avian and animal hosts, including humans. in general, this colonization is either as a commensal, as in birds, or is an asymptomatic transient infection, as in livestock and in humans in endemic regions. however, in susceptible human populations, infection causes acute bacterial enteritis. the ecology of the organism for each outcome of colonization is consider ... | 2002 | 23570169 |
| molecular cloning and transposition of a kanamycin resistance determinant from campylobacter jejuni between replicons in escherichia coli. | this paper reports a restriction map of a fragment of dna encoding kanamycin resistance cloned from plasmid dna of campylobacter jejuni aba94 in the recombinant plasmid prs9421-1. in transposition experiments, kanamycin-resistant r751::km9421 transconjugants appeared at frequencies of 10(-7) per donor cell. these transconjugants harboured a plasmid 4 kb larger than the parental 49 kb plasmid r751. restriction enzyme analysis and southern blot hybridization of these transconjugants showed that th ... | 1996 | 24415309 |
| cloning and expression of the erythromycin resistance determinant from campylobacter jejuni in escherichia coli. | the erythromycin resistance gene (em(r)) from campylobacter jejuni aba94 plasmid dna was cloned into the puc18 vector and then expressed in escherichia coli. the location of the em(r) determinant on the chimeric plasmid was determined by restriction endonuclease mapping within a 0.8-kb ecori fragment. this fragment then hybridized to the 78-kb plasmid dna but not to the 3.3-or 12.6-kb plasmid dna of campylobacter jejuni aba94. em(r) in campylobacter jejuni is therefore probably plasmid-mediated. | 1995 | 24415023 |
| guillain-barré syndrome with severe persistent disability: relationship to hyperacute guillain-barré syndrome. | guillain-barré syndrome (gbs) is a heterogeneous condition with a variable prognosis. we studied nine patients who were unable to walk unaided 12 months after the onset of their illness to discover whether they belonged to a more homogeneous subgroup. sis of the nine patients had symptoms of gastroenteritis shortly before the onset of their neuropathic symptoms and a hyperacute onset of weakness so that they were bed-bound within 24 h. these patients had predominantly motor rather than sensory i ... | 1994 | 24283425 |
| changes in cell morphology and polyamine composition during growth of campylobacter jejuni. | resting cells of campylobacter jejuni were spherical whereas growing cells were mainly spiral. content of cadaverine increased with the decrease in spherical forms prior to growth commencing but production of spermidine increased in early log phase. cadaverine and spermidine are possibly involved in changes in cell morphology and growth, respectively. | 1994 | 24421028 |
| waterborne transmission ofcampylobacter enteritis. | campylobacter jejuni is an important cause of human diarrheal disease throughout the world and likesalmonella enteritidis, has a large animal reservoir which includes most of man's domestic animals. until recently, it has been difficult to trace the chain of transmission from animals to man because of inadequate environmental sampling techniques and means to distinguish strains. recent improvements in these techniques have made environmental studies more feasible in 2 water-related out-breaks.in ... | 1982 | 24226052 |
| high prevalence and predominance of the aph(2″)-if gene conferring aminoglycoside resistance in campylobacter. | campylobacter is a major foodborne pathogen and previous studies revealed that campylobacter isolates from food-producing animals are increasingly resistant to gentamicin in china. the molecular epidemiology and genetic mechanisms responsible for gentamicin resistance in china have not been well understood. in this study, 607 campylobacter isolates of chicken and swine origins collected in the year of 2014 were analyzed, revealing that 15.6% (25/160) of the c. jejuni isolates and 79.9% (357/447) ... | 2017 | 28264854 |
| high rates of contamination of poultry meat products with drug resistant campylobacter in metro manila, philippines. | a total of 265 chicken parts were collected from 15 wet markets and 15 supermarkets in metro manila, philippines. campylobacter spp. was isolated on modified charcoal cefoperazone deoxycholate agar plates and identified through biochemical tests and pcr amplification of genus and species specific genes. antimicrobial resistance profiles were determined following clinical and laboratory standards institute protocols. two hundred seven (78%) campylobacter spp. were isolated. c. jejuni and c. coli ... | 2016 | 27795478 |
| development of an isothermal amplification-based assay for the rapid visual detection of salmonella bacteria. | the efficient and timely detection of pathogens is a major concern worldwide. the aim of this study was to establish a rapid detection method for salmonella bacteria in food samples to facilitate timely treatment. widely used detection methods currently include culture-based methods and pcr-based methods. the former are time consuming, requiring 2 to 3 d, whereas the latter have higher accuracy but are typically complicated, requiring expertise and expensive instruments. in this study, a sensiti ... | 2017 | 28711269 |
| a rapid salmonella detection method involving thermophilic helicase-dependent amplification and a lateral flow assay. | salmonella is a major foodborne pathogen that is widespread in the environment and can cause serious human and animal disease. since conventional culture methods to detect salmonella are time-consuming and laborious, rapid and accurate techniques to detect this pathogen are critically important for food safety and diagnosing foodborne illness. in this study, we developed a rapid, simple and portable salmonella detection strategy that combines thermophilic helicase-dependent amplification (thda) ... | 2017 | 28526589 |
| development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens. | bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. in this study a novel assay, combining gold nanoparticle (gnp)-based multiplex oligonucleotide ligation-pcr and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including shigella spp., campylobacter jejuni, bacillus cereus, escherichia coli o157:h7, listeria monocyt ... | 2017 | 28505584 |
| next generation sequencing-based multigene panel for high throughput detection of food-borne pathogens. | contamination of food by chemicals or pathogenic bacteria may cause particular illnesses that are linked to food consumption, commonly referred to as foodborne diseases. bacteria are present in/on various foods products, such as fruits, vegetables and ready-to-eat products. bacteria that cause foodborne diseases are known as foodborne pathogens (fbps). accurate detection methods that are able to reveal the presence of fbps in food matrices are in constant demand, in order to ensure safe foods wi ... | 2017 | 28578266 |
| campylobacter jejuni as a cause of diarrhoea. | this study was carried out to isolate and identify campylobacter jejuni from stool samples of patients suffering from diarrhoea. the study spanned a two-year period and c. jejuni was isolated in 10 (4.5%) out of 220 patients of diarrhoea] disorders. in samples of stool collected from 50 healthy individuals, c. jejuni was isolated in one (2%) case. further, to isolate other enteropathogens alongwith c. jejuni, procedures were undertaken simultaneously to identify salmonella, shigella and vibrio c ... | 1997 | 28769509 |
| application of maldi-tof ms fingerprinting as a quick tool for identification and clustering of foodborne pathogens isolated from food products. | foodborne pathogens can be associated with a wide variety of food products and it is very important to identify them to supply safe food and prevent foodborne infections. since traditional techniques are time-consuming and laborious, this study was designed for rapid identification and clustering of foodborne pathogens isolated from various restaurants in al-qassim region, kingdom of saudi arabia (ksa) using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ... | 2017 | 28825446 |
| application of long amplicon propidium monoazide-pcr to assess the effects of temperature and background microbiota on pathogens in river water. | the decay rates of enteric waterborne pathogens were evaluated following the introduction of yersinia enterocolitica, salmonella enterica, campylobacter jejuni and arcobacter butzleri into river water at different temperatures (5, 15 and 25°c) for a period of 28 days. to improve the accuracy of the results a molecular viability assay, long amplicon propidium monoazide-polymerase chain reaction (pma-pcr), was used to quantify the viable cell concentration and results from pcr with and without pma ... | 2017 | 28598346 |
| probiotic characteristics of lactobacillus curvatus dn317, a strain isolated from chicken ceca. | the probiotic characteristics of lactobacillus curvatus dn317, a strain isolated from chicken ceca, were evaluated. this strain was selected for study from the isolated lactobacillus strains because it has specific anti-microbial activity against campylobacter jejuni atcc 33560, camp. jejuni nctc 11168, listeria monocytogenes atcc 7644, and bacillus subtilis atcc 8633. lact. curvatus dn317 showed an auto-aggregation percentage of 72% and presented the highest co-aggregation with lact. monocytoge ... | 2017 | 28741151 |
| synthesis and antimicrobial activity of chiral quaternary n-spiro ammonium bromides with 3',4'-dihydro-1'h-spiro[isoindoline-2,2'-isoquinoline] skeleton. | a new class of highly functionalized tetrahydroisoquinolines with a quaternary carbon stereocenter was synthesized starting from an easily accessible l-tartaric acid. nine strains of bacteria (staphylococcus aureus, streptococcus pyogenes, streptococcus mutans, streptococcus salivarius, bacillus subtilis, enterococcus faecalis, moraxella catarrhalis, escherichia coli, campylobacter jejuni) were used for the determination of minimal inhibitory concentration (mic) and minimal bactericidal concentr ... | 2017 | 28740363 |
| amelioration of intestinal and systemic sequelae of murine campylobacter jejuni infection by probiotic vsl#3 treatment. | the incidence of human campylobacter jejuni infections is progressively increasing worldwide. probiotic compounds might open up valuable tools to decrease pathogen burden and subsequent pro-inflammatory immune responses, but in vivo data are scarce. | 2017 | 28413453 |
| biochemical characterization of wbkc, an n-formyltransferase from brucella melitensis. | it has become increasingly apparent within the last several years that unusual n-formylated sugars are often found on the o-antigens of such gram negative pathogenic organisms as francisella tularensis, campylobacter jejuni, and providencia alcalifaciens, among others. indeed, in some species of brucella, for example, the o-antigen contains 1,2-linked 4-formamido-4,6-dideoxy-α-d-mannosyl groups. these sugars, often referred to as n-formylperosamine, are synthesized in pathways initiating with gd ... | 2017 | 28636341 |
| prevalence and risk factors for thermotolerant species of campylobacter in poultry meat at retail in europe. | the thermotolerant species campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter upsaliensis are the causative agents of the human illness called campylobacteriosis. this infection represents a threat for the health of consumers in europe. it is well known that poultry meat is an important food vehicle of campylobacter infection. as emerged from the reported scientific literature published between 2006 and 2016, poultry meat sold at retail level in europe represents an i ... | 2017 | 28854745 |
| isolation and identification of campylobacter spp. from poultry and poultry by-products in tunisia by conventional culture method and multiplex real-time pcr. | thermophilic campylobacter spp. are one of the primary causes of bacterial human diarrhea. the consumption of poultry meats, by-products, or both is suspected to be a major cause of human campylobacteriosis. the aims of this study were to determine the prevalence of thermophilic campylobacter spp. in fresh poultry meat and poultry by-products by conventional culture methods and to confirm campylobacter jejuni and campylobacter coli isolates by using the multiplex pcr assay. two hundred fifty fre ... | 2017 | 28853632 |
| genome reduction for niche association in campylobacter hepaticus, a cause of spotty liver disease in poultry. | the term "spotty liver disease" (sld) has been used since the late 1990s for a condition seen in the uk and australia that primarily affects free range laying hens around peak lay, causing acute mortality and a fall in egg production. a novel thermophilic sld-associated campylobacter was reported in the united kingdom (uk) in 2015. subsequently, similar isolates occurring in australia were formally described as a new species, campylobacter hepaticus. we describe the comparative genomics of 10 c. ... | 2017 | 28848714 |
| fecal shedding of campylobacter jejuni and campylobacter coli among feral pigs in texas. | the population and range of feral pigs in the united states are rapidly expanding, yet key knowledge gaps exist regarding their role in the ecology and transmission of foodborne pathogens. our objectives were to estimate the prevalence of campylobacter jejuni and campylobacter coli shedding among feral pigs throughout texas and to identify risk factors for positive status. faecal samples were collected from feral pigs in texas from february 2014 through may 2015, and target organisms were detect ... | 2017 | 28834190 |
| cardamom, cumin, and dill weed essential oils: chemical compositions, antimicrobial activities, and mechanisms of action against campylobacter spp. | natural antimicrobials as well as essential oils (eos) have gained interest to inhibit pathogenic microorganisms and to control food borne diseases. campylobacter spp. are one of the most common causative agents of gastroenteritis. in this study, cardamom, cumin, and dill weed eos were evaluated for their antibacterial activities against campylobacter jejuni and campylobacter coli by using agar-well diffusion and broth microdilution methods, along with the mechanisms of antimicrobial action. che ... | 2017 | 28714890 |
| genetic diversity of thermotolerant campylobacter spp. isolates from different stages of the poultry meat supply chain in argentina. | the objective of this study was to investigate a clonal relationship among thermotolerant campylobacter spp. isolates from different stages of the poultry meat supply chain in argentina. a total of 128 thermotolerant campylobacter spp. (89 c. jejuni and 39 c. coli) isolates from six poultry meat chains were examined. these isolates were from: a) hens from breeder flocks, b) chickens on the farm (at ages 1 wk and 5 wk), c) chicken carcasses in the slaughterhouse, and d) chicken carcasses in the r ... | 2017 | 28712509 |
| a study on campylobacter jejuni and campylobacter coli through commercial broiler production chains in thailand: antimicrobial resistance, the characterization of dna gyrase subunit a mutation, and genetic diversity by flagellin a gene restriction fragment length polymorphism. | contaminated poultry meat is regarded as the main source of human campylobacteriosis. during september 2014 and february 2015, breeder flocks, hatcheries, and broiler farms from two chicken production chains were investigated chronologically. five commercial breeder flocks (breeder flocks 1-5), two hatcheries (hatcheries a and b), and five broiler flocks (broiler flocks 1-5) were sampled in this study. campylobacter colonization of both breeder and broiler flocks was determined from cloacal swab ... | 2017 | 28665716 |
| campylobacter species isolated from pigs in grenada exhibited novel clones: genotypes and antimicrobial resistance profiles of sequence types. | infections caused by campylobacter species pose a severe threat to public health worldwide. however, in grenada, the occurrence and characteristics of campylobacter in food animals, including pigs, remain mostly unknown. in this study, we identified the sequence types (sts) of campylobacter from young healthy pigs in grenada and compared the results with previous studies in grenada and other countries. antimicrobial resistance patterns and diversity of the campylobacter clones were evaluated. ni ... | 2017 | 28650672 |
| impact of built-up-litter and commercial antimicrobials on salmonella and campylobacter contamination of broiler carcasses processed at a pilot mobile poultry-processing unit. | the small-scale mobile poultry-processing unit (mppu) produced raw poultry products are of particular food safety concern due to exemption of usda poultry products inspection act. limited studies reported the microbial quality and safety of mppu-processed poultry carcasses. this study evaluated the salmonella and campylobacter prevalence in broiler ceca and on mppu-processed carcasses and efficacy of commercial antimicrobials against campylobacter jejuni on broilers. in study i, straight-run hub ... | 2017 | 28649571 |