| characterization and partial purification of a bacteriocin-like substance produced by thermophilic bacillus licheniformis h1 isolated from cow manure compost. | the production and partial characterization of bacteriocin-like substances (blss) produced by bacteria isolated from cow manure compost were investigated. eight bls producers, which exhibited inhibitory activity against pathogenic bacteria, were isolated from cow manure compost at different stages of the composting process. the pile temperature ranged from 9.1 -¦c to 73.2 -¦c. the blss showed thermostability, but the bls producers were not thermostable except for the h1 producer. thermophilic ba ... | 2011 | 21729216 |
| signal 3 requirement for memory cd8(+) t-cell activation is determined by the infectious pathogen. | the relevance of direct inflammatory signals (signal 3) for the activation of memory cd8(+) t cells during recall responses is so far unknown. we therefore investigated the direct impact of il-12 and type i ifn on the formation, recall potential and protective capacity of memory t cells. using cd8(+) t cells deficient for il-12 or type i ifn receptors in an adoptive transfer system, we generated memory populations after infection with vaccinia virus, lymphocytic choriomeningitis virus or listeri ... | 2011 | 21830209 |
| prime-boost vaccination with heterologous live vectors encoding siv gag and multimeric hiv-1 gp160 protein: efficacy against repeated mucosal r5 clade c shiv challenges. | we sought to induce primate immunodeficiency virus-specific cellular and neutralizing antibody (nab) responses in rhesus macaques (rm) through a bimodal vaccine approach. rm were immunized intragastrically (i.g.) with the live-attenuated listeria monocytogenes (lm) vector lmdd-bdopsivgag encoding sivmac239 gag. siv gag-specific cellular responses were boosted by intranasal and intratracheal administration of replication-competent adenovirus (ad5hr-sivgag) encoding the same gag. to broaden antivi ... | 2011 | 21693155 |
| [listeria monocytogenes in human infections]. | the listeria genus is distinguished into six species from which just one--listeria monocytogenes is pathogenic for humans. the main route of acquisition of listeria is through the ingestion of contaminated food products. an important element of the l. monocytogenes pathogenesis infection is affiliation with high-risk group of immunocompromised patients, infants or pregnant women, who infected by this microorganism can lead to miscarriage. listeriosis can appear in the form of sepsis, infection o ... | 2011 | 21735837 |
| the use of hollow fiber membranes combined with cytometry in analysis of bacteriological samples. | to avoid destruction of the implanted biological material it may be separated from host immunological system by enclosure within a permiselective membrane. two-directional diffusion through the membrane of nutrients, metabolic products, as well as bioactive products of encapsulated cells is required to ensure their survival and functional activities. the system of cells encapsulated within the membrane releasing the biologically active substance may be applied either locally to give an opportuni ... | 2011 | 21704848 |
| microbial challenges of poultry meat production. | food safety and shelf-life are both important microbial concerns in relation to broiler meat production. focus is mainly placed on the absence or control of potentially pathogenic microbes such as salmonella spp. and campylobacter spp. but, from the commercial point of view, other spoilage bacteria also play a role as potential threats. regarding food safety, the primary target should be the production of pathogen-free live animals, thus allowing slaughter plants to keep the processing line free ... | 2011 | 21718794 |
| n-terminal gly(224)-gly(411) domain in listeria adhesion protein interacts with host receptor hsp60. | listeria adhesion protein (lap) is a housekeeping bifunctional enzyme consisting of n-terminal acetaldehyde dehydrogenase (aldh) and c-terminal alcohol dehydrogenase (adh). it aids listeria monocytogenes in crossing the epithelial barrier through a paracellular route by interacting with its host receptor, heat shock protein 60 (hsp60). to gain insight into the binding interaction between lap and hsp60, lap subdomain(s) participating in the hsp60 interaction were investigated. | 2011 | 21738582 |
| identity, regulation and in vivo function of gut nkp46+ror+¦t+ and nkp46+ror+¦t- lymphoid cells. | the gut is a major barrier against microbes and encloses various innate lymphoid cells (ilcs), including two subsets expressing the natural cytotoxicity receptor nkp46. a subset of nkp46(+) cells expresses retinoic acid receptor-related orphan receptor +¦t (ror+¦t) and produces il-22, like lymphoid tissue inducer (lti) cells. other nkp46(+) cells lack ror+¦t and produce ifn-+¦, like conventional natural killer (cnk) cells. the identity, the regulation and the in vivo functions of gut nkp46(+) il ... | 2011 | 21685873 |
| lmo0036, an ornithine and putrescine carbamoyltransferase in listeria monocytogenes, participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance. | listeria monocytogenes is a foodborne pathogen causing listeriosis. acid is one of the most frequently stresses that foodborne pathogens encounter. the ability to survive and proliferate in acidic environments is a prerequisite for its infection. however, there is limited knowledge about the molecular basis of adaption of l. monocytogenes to acid. arginine deiminase (adi) and agmatine deiminase (agdi) systems are implicated in bacterial tolerance to acidic environments. homologues of adi and agd ... | 2011 | 21835877 |
| transcriptome analysis of listeria monocytogenes grown on a ready-to-eat meat matrix. | the contamination of ready-to-eat (rte) meat products with listeria monocytogenes is a major concern for the food industry. for a better understanding of the adaptation and survival ability of l. monocytogenes grown on turkey deli meat, the transcriptome of l. monocytogenes strain f2365 was determined with a microarray. microarray data were validated with a quantitative real-time reverse transcription pcr assay. based on the microarray data, 39 and 45 genes from l. monocytogenes were transcripti ... | 2011 | 21740712 |
| recruitment of the major vault protein by inlk: a listeria monocytogenes strategy to avoid autophagy. | l. monocytogenes is a facultative intracellular bacterium responsible for listeriosis. it is able to invade, survive and replicate in phagocytic and non-phagocytic cells. the infectious process at the cellular level has been extensively studied and many virulence factors have been identified. yet, the role of inlk, a member of the internalin family specific to l. monocytogenes, remains unknown. here, we first show using deletion analysis and in vivo infection, that inlk is a bona fide virulence ... | 2011 | 21829365 |
| streptolysin s-like virulence factors: the continuing saga. | streptolysin s (sls) is a potent cytolytic toxin and virulence factor that is produced by nearly all streptococcus pyogenes strains. despite a 100-year history of research on this toxin, it has only recently been established that sls is just one of an extended family of post-translationally modified virulence factors (the sls-like peptides) that are produced by some streptococci and other gram-positive pathogens, such as listeria monocytogenes and clostridium botulinum. in this review, we descri ... | 2011 | 21822292 |
| role of flha and mota in growth of listeria monocytogenes at low temperatures. | while temperature-dependent induction of flagella is a well-characterized phenomenon in listeria monocytogenes, the essentiality of increased flagellum production during growth at low temperatures remains unclear. to study this relationship, we compared the relative expression levels of two motility genes, flha and mota, at 3┬░c, 25┬░c and 37┬░c in l. monocytogenes strain egd-e by using qrt-pcr, and compared the growth curves, motility, and flagellation between the wild-type and flha and mota de ... | 2011 | 21683466 |
| quantitative risk assessment of listeriosis due to consumption of raw milk. | the objectives of this study were to estimate the risk of illness for raw milk consumers due to listeria monocytogenes in raw milk sold by permitted dealers, and the risk for people on farms who consume raw milk. three scenarios were evaluated for raw milk sold by dealers: raw milk purchased directly from bulk tanks, from on-farm stores, and from retail. to assess the effect of mandatory testing of raw milk by regulatory agencies, the number of listeriosis cases per year was compared where no ra ... | 2011 | 21819653 |
| cd8+ t cells primed in the periphery provide time-bound immune-surveillance to the central nervous system. | after vaccination, memory cd8(+) t cells migrate to different organs to mediate immune surveillance. in most nonlymphoid organs, following an infection, cd8(+) t cells differentiate to become long-lived effector-memory cells, thereby providing long-term protection against a secondary infection. in this study, we demonstrated that ag-specific cd8(+) t cells that migrate to the mouse brain following a systemic listeria infection do not display markers reminiscent of long-term memory cells. in cont ... | 2011 | 21715683 |
| antimicrobial susceptibilities of listeria monocytogenes isolated in japan. | the antimicrobial susceptibility of 201 listeria monocytogenes isolates from foods, environments, animals and human patients in japan was determined. all isolates were susceptible to ampicillin, the first choice of drug for listeriosis treatment, chloramphenicol, dihydrostreptomycin, erythromycin, enrofloxacin, gentamicin, kanamycin, lincomycin, nosiheptide, salinomycin, vancomycin, and virginiamycin. a human strain was resistant to oxytetracycline. the minimum inhibitory concentration (mic) for ... | 2011 | 21836384 |
| effect of lactoferrin and its derivatives against gram-positive bacteria in vitro and, combined with high pressure, in chicken breast fillets. | the bactericidal activity of lactoferrin (lf), amidated lactoferrin (amilf), pepsin digested lactoferrin (pdlf), and its activated (alf) commercial form, against six strains of three gram-positive bacterial species was investigated. listeria monocytogenes was most sensitive in vitro, staphylococcus aureus showed a moderate resistance, and enterococus faecalis was highly resistant to antimicrobials. when chicken breast fillets were inoculated with l. monocytogenes cect5725 and treated with antimi ... | 2011 | 21703778 |
| inactivation of trea, the gene encoding a phosphotrehalase, causes increased thermal and osmotic stress resistance in listeria monocytogenes 568 when grown in the presence of trehalose. | the foodborne pathogen, listeria monocytogenes is a problem for food processors and consumers alike, as the organism is resistant to harsh environmental conditions and inimical barriers implemented to prevent the survival and/or growth of harmful bacteria. one mechanism by which listeriae mediates survival is through the accumulation of compatible solutes, such as proline, betaine and carnitine. in other bacteria including escherichia coli, the synthesis and accumulation of another compatible so ... | 2011 | 21821737 |
| effect of antimicrobial coatings on the radiosensitization of escherichia coli, salmonella typhimurium, and listeria monocytogenes in fresh broccoli. | radiosensitization of listeria monocytogenes, escherichia coli, salmonella typhimurium, and aerobic microflora was evaluated in broccoli florets coated by antimicrobial coatings and treated with different doses of +¦-radiation. broccoli florets were inoculated with aerobic microflora isolated from broccoli and with pathogenic bacteria (l. monocytogenes, e. coli, and salmonella typhimurium) at 10(6) cfu/ml. inoculated florets were then coated with methylcellulose-based coating containing various ... | 2011 | 21740707 |
| transient receptor potential melastatin 2 (trpm2) ion channel is required for innate immunity against listeria monocytogenes. | the generation of reactive oxygen species (ros) is inherent to immune responses. ros are crucially involved in host defense against pathogens by promoting bacterial killing, but also as signaling agents coordinating the production of cytokines. transient receptor potential melastatin 2 (trpm2) is a ca(2+)-permeable channel gated via binding of adp-ribose, a metabolite formed under conditions of cellular exposure to ros. here, we show that trpm2-deficient mice are extremely susceptible to infecti ... | 2011 | 21709234 |
| inlb-mediated listeria monocytogenes internalization requires a balanced phospholipase d activity maintained through phospho-cofilin. | internalization of listeria monocytogenes into non-phagocytic cells is tightly controlled by host cell actin dynamics and cell membrane alterations. however, knowledge about the impact of phosphatidylcholine cleavage driven by host cell phospholipase d (pld) on listeria internalization into epithelial cells is limited. here, we report that l. monocytogenes activates pld in vero cells during the internalization. with immunostaining it was shown that both pld1 and pld2 surrounded partially or comp ... | 2011 | 21722201 |
| oata, a peptidoglycan o-acetyltransferase involved in listeria monocytogenes immune escape, is critical for virulence. | microbial pathogens have evolved mechanisms to overcome immune responses and successfully infect their host. here, we studied how listeria monocytogenes evades immune detection by peptidoglycan (pgn) modification. by analyzing l. monocytogenes muropeptides, we detected o-acetylated muramic acid residues. we identified an o-acetyltransferase gene, oata, in the l. monocytogenes genome sequence. comparison of pgn from parental and isogenic oata mutant strains showed that the o-acetyltransferase oat ... | 2011 | 21844299 |
| prevalence and biofilm-forming ability of listeria monocytogenes in new zealand mussel (perna canaliculus) processing plants. | greenshellôäó mussels are new zealand's largest seafood export species. some export markets require compliance with 'zero' tolerance legislation for listeria monocytogenes in 25-ág of product. even though individually quick frozen (iqf) mussel products are labeled 'to be cooked', and are not classified as ready-to-eat, some markets still require them to comply with the strict policy. three mussel processing plants were assessed for the pattern of l.-ámonocytogenes contamination on raw material, ... | 2011 | 21839390 |
| characterization and antibiotic susceptibility of listeria monocytogenes isolated from poultry and red meat in morocco. | this study was carried out on 426 samples of raw meats collected from butcheries and supermarkets in casablanca, morocco. the samples were examined for the occurrence of listeria species. strains of listeria monocytogenes were characterized by several biochemical tests and confirmed by polymerase chain reaction (pcr). +¦-hemolytic cultures and nonhemolytic isolates were tested for biochemical properties with the listeria api test. among the 43 listeria species isolates; we identified 10 strains ... | 2008 | 21694879 |
| novel multiplex single nucleotide polymorphism-based method for identifying epidemic clones of listeria monocytogenes. | a novel primer extension-based, multiplex minisequencing assay targeting six highly informative single nucleotide polymorphisms (snps) in four virulence genes correctly identified and differentiated all four epidemic clones (ecs) of listeria monocytogenes and 9 other strains initially misclassified as non-ecs. this assay allows rapid, accurate, and high-throughput screening for all known ecs of l. monocytogenes. | 2011 | 21742911 |
| prevalence of foodborne pathogens in grilled chicken from street vendors and retail outlets in reynosa, tamaulipas, mexico. | we analyzed a total of 70 grilled chicken samples bought randomly from street vendors and retail outlets in the city of reynosa, mexico, to determine the prevalence of escherichia coli (shiga toxin producing and enterotoxin producing), salmonella spp., staphylococcus aureus, listeria spp., and campylobacter spp. using microbiological methods and pcr detection of bacterial sequences. of the 70 samples, 27 (38.5%) were from retail outlets and 43 (61.4%) from street vendors. all specimens were nega ... | 2011 | 21819659 |
| influence of synthetic peptide inducers on antibacterial activity of enterococci. | antibacterial activity of probiotic enterococci is often related to the production of bacteriocins. there is a variety of enterococcus faecium strains which synthesise one or several enterocins including a, b, p, 96, l50ab and others. in this work we have analysed the probiotic strain e. faecium l3 whose genome contained the genes encoding enterocins ðé, ðæ and a three-component regulatory system. all these genes were found to be expressed in enterococcal strain l3. antimicrobial activity of the ... | 2011 | 21840807 |
| [listeriosis of the central nervous system: epidemiological and diagnostic aspects--own observations]. | the aim of the study was to evaluate clinical and laboratory findings and predisposing factors for listeria monocytogenes (lm) meningitis. | 2011 | 21735838 |
| prevalence, distribution, and diversity of listeria monocytogenes in retail environments, focusing on small establishments and establishments with a history of failed inspections. | despite growing concerns about cross-contamination of ready-to-eat foods with listeria monocytogenes, our knowledge about the ecology and transmission of l. monocytogenes in retail establishments has remained limited. we conducted a cross-sectional study to characterize the prevalence, distribution, and subtype diversity of l. monocytogenes in 120 new york state retail deli establishments that were hypothesized to present an increased risk for environmental l. monocytogenes contamination (i.e., ... | 2011 | 21740710 |
| tlr2 signaling contributes to rapid inflammasome activation during f. novicida infection. | background: early detection of microorganisms by the innate immune system is provided by surface-expressed and endosomal pattern recognition receptors (prrs) such as toll-like receptors (tlrs). detection of microbial components by tlrs initiates a signaling cascade leading to the expression of proinflammatory cytokines including il-6 and il-1+¦. some intracellular bacteria subvert the tlr response by rapidly escaping the phagosome and entering the cytosol. however, these bacteria may be recogniz ... | 2011 | 21698237 |
| comparison between the resistance of benzalkonium chloride-adapted and -nonadapted biofilms of listeria monocyogenes to modified atmosphere packaging and nisin once transferred to mussels. | benzalkonium chloride-adapted and -nonadapted listeria monocytogenes biofilm cells were transferred by contact to cooked or live mussels and packed in rich co(2) and o(2), respectively. the viabilities of transferred cells during storage of these packed samples at 2.5 -¦c were compared. in addition, in cooked mussels the combined effect of co(2) and nisin against the survival of l. monocytogenes was also studied by using a first-order factorial design. the results obtained demonstrated that biof ... | 2011 | 21740713 |
| regulated shift from helical to polar localization of listeria monocytogenescell wall-anchored proteins. | many virulence factors of gram-positive bacterial pathogens are covalently anchored to the peptidoglycan (pg) by sortase enzymes. however, for rod-shaped bacteria little is known about the spatiotemporal organization of these surface proteins in the cell wall. here we report the three-dimensional (3d) localization of the pg-bound virulence factors inla, inlh, inlj, and svpa in the envelope of listeria monocytogenesunder different growth conditions. we found that all pg-anchored proteins are posi ... | 2011 | 21725001 |
| acid adaptation affects the viability of listeria monocytogenes bcrc 14846 and salmonella typhimurium bcrc 10747 exposed to disinfectants at 25-¦c and 40-¦c. | abstract in the present study, listeria monocytogenes bcrc 14846 and salmonella typhimurium bcrc 10747 were subjected to acid adaptation at ph 5.5 at 37-¦c for 1 and 4ôçëh, respectively. the viability of the acid-adapted cells of test organisms exposed to clidox-s, a chlorine-containing disinfectant, and quatricide, a quaternary ammonium compound, was examined and compared with that of the control cells at 25-¦c and 40-¦c. results revealed that acid adaptation significantly enhanced the viabilit ... | 2011 | 21740275 |
| identification of bacteriocin genes in enterococci isolated from game animals and saltwater fish. | bacteriocins produced by enterococci, referred to as enterocins, possess great interest for their potential use as biopreservatives in food and feed, as well as alternative antimicrobials in humans and animals. in this context, the aim of the present study was to determine the antimicrobial activity and the presence of bacteriocin structural genes in fecal enterococcal isolates from animal origins. evaluation of the direct antimicrobial activity of 253 isolates from wild boars (sus scrofa, n = 6 ... | 2011 | 21819651 |
| reduced detectability of listeria monocytogenes in the presence of listeria innocua. | recent foodborne crises have demonstrated the importance of monitoring food safety. in terms of microbiological criteria, food safety requires the reliable detection of pathogens such as listeria monocytogenes along the food chain by appropriate analytical methods. however, indications exist that accompanying listeria innocua strains suppress the growth of l. monocytogenes during selective enrichment, which may cause reduced or even inhibited detection. to study these effects, the limit of detec ... | 2011 | 21819654 |
| behaviour of listeria monocytogenes in packaged water buffalo mozzarella cheese. | aims:ôçé the aim of the study was to evaluate the behaviour of listeria monocytogenes in the conditioning liquid of packaged water buffalo mozzarella cheese (wbmc). methods and results:ôçé the conditioning liquid was contaminated with l.-ámonocytogenes, and the contaminated samples were stored at four different storage temperatures: 5 and 10-¦c for 22ôçâdays; 20-¦c for 9ôçâdays; 20-¦c for 3ôçâdays and then at 5-¦c for 6ôçâdays. the results showed that l.-ámonocytogenes concentration decreased wh ... | 2011 | 21740447 |
| evaluation of beneficial attributes for phytate-degrading pediococcus pentosaceus cfr r123. | in this study we have screened and selected potent phytate-degrading lactic acid bacteria (lab), and evaluated their beneficial attributes. around 60 lab strains were isolated from several cereal- and pulse-based conventional fermented preparations. they were screened for their ability to degrade myo-inositol hexakisphosphate (ip6) by cobalt chloride qualitative staining method (plate assay). one of the cultures, pediococcus pentosaceus cfr r123, was capable of degrading both calcium and sodium ... | 2010 | 21831762 |
| innate memory phenotype cd4(+) t cells play a role in early protection against infection with listeria monocytogenes in a cd30l-dependent manner. | cd30 ligand (cd30l, cd153) is a type ii membrane-associated glycoprotein belonging to the tumor necrosis factor (tnf) family. we show here that cd30l knock out (ko) mice were highly susceptible to primary infection with listeria monocytogenes as assessed by the survival rate. the number of bacteria on day 3 after infection was significantly greater in the peritoneal cavity, spleen and liver of cd30lko mice than in wild type (wt) mice. the innate function of memory phenotype (mp) cd44(+) cd4(+) t ... | 2011 | 21699557 |
| listeria bacteriophage peptidoglycan hydrolases feature high thermoresistance and reveal increased activity after divalent metal cation substitution. | the ability of the bacteriophage-encoded peptidoglycan hydrolases (endolysins) to destroy gram-positive bacteria from without makes these enzymes promising antimicrobials. recombinant endolysins from listeria monocytogenes phages have been shown to rapidly lyse and kill the pathogen in all environments. to determine optimum conditions regarding application of recombinant listeria phage endolysins in food or production equipments, properties of different listeria endolysins were studied. optimum ... | 2011 | 21720825 |
| detection, cellular localization and antibacterial activity of two lytic enzymes of pediococcus acidilactici atcc 8042. | aim:ôçé in pediococcus acidilactici atcc 8042, two activities of peptidoglycan hydrolase (pgh) with lytic effect against micrococcus lysodeikticus and staphylococcus aureus have been detected. this work intends to elucidate the growth phase of maximum lytic activity, the localization and the effectiveness of the activity against pathogenic gram-negative and gram-positive bacteria. methods and results:ôçé cells were grown in mrs medium and collected at different growth stages, and the proteins we ... | 2011 | 21707881 |
| variability in biofilm production by listeria monocytogenes correlated to strain origin and growth conditions. | this study aimed to identify factors that influence the development of biofilm by listeria monocytogenes strains and to determine the extent to which biofilm production protects against quaternary ammonium compound (qac) disinfectant challenge. a total of 95 l. monocytogenes strains were studied and biofilm production was assessed as a function of incubation temperature, media ph, strain origin, serotype, and environmental persistence status. attachment and biofilm development (inferred by the l ... | 2011 | 21824672 |
| cutting edge: {beta}-catenin is dispensable for t cell effector differentiation, memory formation, and recall responses. | the molecular mechanisms that regulate mature t cell fate and enable cells to differentiate into memory t cells are largely unknown. memory t cells share certain key features with stem cells: they both have the ability to self-renew and are long-lived. the wnt-+¦-catenin signaling pathway is a key player in regulating stem cell self-renewal and differentiation. we generated a conditional knockout mouse that specifically lacks +¦-catenin in mature t cells and report in this article that +¦-cateni ... | 2011 | 21724993 |
| rhombencephalitis: a series of 97 patients. | the term rhombencephalitis refers to inflammatory diseases affecting the hindbrain (brainstem and cerebellum). rhombencephalitis has a wide variety of etiologies, some of them potentially severe and life threatening without proper early treatment. in this retrospective observational study, we reviewed the records of consecutively hospitalized patients at university hospital of bellvitge (barcelona, spain) from january 1990 to december 2008. rhombencephalitis was defined as a brainstem and/or cer ... | 2011 | 21694648 |
| efficacy of freezing, frozen storage and edible antimicrobial coatings used in combination for control of listeria monocytogenes on roasted turkey stored at chiller temperatures. | the presence and growth of listeria monocytogenes on ready-to-eat (rte) turkey is an important food safety issue. the antilisterial efficacy of four polysaccharide-based edible coatings (starch, chitosan, alginate and pectin) incorporating sodium lactate (sl) and sodium diacetate (sd) as well as commercial preparations opti.form pd4, novagardôäó cb1, protect-m and guardianôäó nr100 were compared against l.┬ámonocytogenes on roasted turkey. pectin coating treatments incorporating sl/sd, opti.form ... | 2011 | 21839391 |
| a protein thermometer controls temperature-dependent transcription of flagellar motility genes in listeria monocytogenes. | facultative bacterial pathogens must adapt to multiple stimuli to persist in the environment or establish infection within a host. temperature is often utilized as a signal to control expression of virulence genes necessary for infection or genes required for persistence in the environment. however, very little is known about the molecular mechanisms that allow bacteria to adapt and respond to temperature fluctuations. listeria monocytogenes (lm) is a food-borne, facultative intracellular pathog ... | 2011 | 21829361 |
| genome sequence of the nonpathogenic listeria monocytogenes serovar 4a strain m7. | this report presents the complete and annotated genome sequence of the naturally nonpathogenic listeria monocytogenes serovar 4a strain m7, isolated from cow's milk in zhejiang province, china. | 2011 | 21742872 |
| listeria monocytogenes contamination in french breeding and fattening turkey flocks. | this study aimed to collect data and create a database related to listeria monocytogenes contamination in breeding and fattening turkey flocks. seventy-five breeding turkey flocks and 86 fattening turkey flocks were sampled. three hundred seventy-five and 428 samples were analyzed in breeding and fattening turkey flocks, respectively. l. monocytogenes was detected in 9 of 75 breeding flocks, leading to an estimated prevalence of 12% (95% confidence interval, 4.6-19.4). in fattening turkeys, the ... | 2011 | 21740711 |
| a novel c-terminal mutation resulting in constitutive activation of the listeria monocytogenes central virulence regulatory factor prfa. | the environmental bacterium listeria monocytogenes survives and replicates in a variety of diverse ecological niches that range from the soil to the cytosol of infected mammalian cells. the ability of l. monocytogenes to replicate within an infected host requires the expression of a number of secreted bacterial gene products whose expression is regulated by the transcriptional activator prfa. prfa becomes activated following bacterial entry into host cells, however the mechanism by which this ac ... | 2011 | 21835879 |
| the lmo0501 gene coding for a putative transcription activator protein in listeria monocytogenes promotes growth under cold, osmotic and acid stress conditions. | in listeria monocytogenes egde, the lmo0501 gene locus encodes a protein similar to the mannitol transcription regulator (mltr) protein in bacillus subtilis and bacillus stearothermophilus. in this study we investigated its functional role in l.-ámonocytogenes egde cells in view of growth under different stress conditions. increased lmo0501 gene expression at mrna level was detected in response to cold, osmotic and organic acid stress exposure. an egde +ölmo0501 mutant strain was diminished in g ... | 2011 | 21839374 |
| adaptation of listeria monocytogenes to oxidative and nitrosative stress in ifn-+¦-activated macrophages. | ifn-+¦-activated macrophages are considered to be the primary effector cells in host defense against listeria monocytogenes infections. however despite the induction of the complex host defense mechanisms, survival of l. monocytogenes in activated macrophages is still observed. here we used a whole genome-based transcriptome approach to examine for bacterial genes specifically induced in ifn-+¦-activated macrophages. we demonstrated that cells activated by ifn-+¦ had elevated oxidative and nitro ... | 2011 | 21697010 |
| the genome sequence of the lactic acid bacterium, carnobacterium maltaromaticum atcc 35586 encodes potential virulence factors. | the genus carnobacterium belongs to the lactic acid bacteria and carnobacterium maltaromaticum is commonly found in modified atmosphere packed and vacuum packed fish and meat products as well as in live fish. this species has been described as a fish pathogenic organism but human clinical isolates have only been obtained at one occasion. to investigate the virulence potential we sequenced the entire genome of strain atcc 35586, isolated from a diseased salmon. when comparing the translated gene ... | 2011 | 21704418 |
| a predictive model for the inactivation of listeria innocua in cooked poultry products during postpackage pasteurization. | contamination of listeria monocytogenes in ready-to-eat poultry products poses potential risk of listeriosis to the public. to control the level of listeria contamination, attention has been focused on the postpackage pasteurization of fully cooked poultry products. in this study, we sought to develop a model to predict the thermal inactivation of l. monocytogenes in chicken drumettes during postpackage hot water pasteurization. fully cooked chicken drumettes were inoculated with listeria innocu ... | 2011 | 21819652 |
| evidence of autoinduction heterogeneity via expression of the agr system of listeria monocytogenes at the single-cell level. | to investigate if the primary function of the agr system of listeria monocytogenes is to monitor cell density, we followed agr expression in batch cultures, in which the autoinducer concentration was uniform, and in biofilms. expression was heterogeneous, suggesting that the primary function of agr is not to monitor population density. | 2011 | 21724873 |
| effect of antimicrobial spice and herb extract combinations on listeria monocytogenes, staphylococcus aureus, and spoilage microflora growth on cooked ready-to-eat vacuum-packaged shrimp. | two spice and herb extract combinations from galangal (alpinia galanga), rosemary (rosmarinus officinalis), and lemon iron bark (eucalyptus staigerana) were evaluated for their ability to inhibit the growth of inoculated listeria monocytogenes and staphylococcus aureus and naturally present spoilage microflora on cooked ready-to-eat shrimp stored for 16 days at 4 or 8 -¦c. a combination of galangal, rosemary, and lemon iron bark significantly reduced (p < 0.05) levels of aerobic bacteria and lac ... | 2011 | 21740714 |
| characterization of two safe enterococcus strains producing enterocins isolated from egyptian dairy products. | five bacterial cocci isolates were selected from a wide pool of 503 isolates collected from traditional egyptian dairy products on the basis of their inhibitory activities against lactobacillus brevis f145, lactobacillus bulgaricus 340, enterococcus faecium hklhs, listeria ivanovii atcc, listeria innocua cip 80.11 and listeria monocytogenes egde 107776. these 5 isolates were identified as e. faecium tx1330 and e. faecium e980 by 16s rdna amplification and sequencing. the antibacterial activity o ... | 2011 | 21831786 |
| antibacterial effects of enniatins j(1) and j(3) on pathogenic and lactic acid bacteria. | enniatins (ens) are n-methylated cyclohexadepsipeptides, secondary metabolites produced by various species of the genus fusarium. they are known to act as antifungal, antiyeast and antibacterial and to possess antiinsecticidal and phytotoxic properties. in this study we evaluated for the first time the antibiotic effect of pure fractions of en j(1) and j(3) on several pathogenic strains and lactic acid bacteria. the ens j(1) and j(3) were purified from the fermentation extract of fusarium solani ... | 2011 | 21742008 |
| effects of natural antimicrobials on inhibition of listeria monocytogenes and on chemical, physical and sensory attributes of naturally-cured frankfurters. | due to regulations for natural and organic processed meats, sodium nitrite and many antimicrobials cannot be used. therefore, natural and organic processed meats are more susceptible to pathogenic bacterial growth, and natural alternatives to chemical preservatives are needed. inhibition of listeria monocytogenes, and quality characteristics of frankfurters manufactured with 3% cranberry powder, or with 1% or 2% cranberry powder each with either cherry powder (0.6%), lime powder (60mg/kg), or a ... | 2011 | 21723674 |
| rapid analysis of listeria monocytogenes cell wall teichoic acid carbohydrates by esi-ms/ms. | we report the application of electrospray ionization (esi) mass spectrometry for compositional characterization of wall teichoic acids (wta), a major component of gram-positive bacterial cell walls. tandem mass spectrometry (esi-ms/ms) of purified and chemically hydrolyzed monomeric wta components provided sufficient information to identify wta monomers and their specific carbohydrate constituents. a lithium matrix was used for ionization of uncharged wta monomers, and successfully applied to an ... | 2011 | 21738682 |
| [biologic therapy and infections]. | biologic therapy is an important therapeutic arsenal in rheumatic diseases. anti-tnf therapies affect host defenses against infections, since tnf mediates inflammation and modulates cellular immune responses. cases of tuberculosis have been observed in patients treated with tnf antagonists, mainly due to the presence of latent or "dormant" tuberculosis infection (ltb1). other microorganisms responsible for the infectious complications associated with biologic therapy are generally intracellular ... | 2008 | 21794348 |
| genome sequence of listeria monocytogenes scott a, a clinical isolate from a food-borne listeriosis outbreak. | listeria monocytogenes is an opportunistic food-borne pathogen and the causative agent of listeriosis in animals and humans. we present the genome sequence of listeria monocytogenes scott a, a widely distributed and frequently used serovar 4b clinical isolate from the 1983 listeriosis outbreak in massachusetts. | 2011 | 21685277 |
| the ifn-+¦-inducible gtpase, irga6, protects mice against toxoplasma gondii but not against plasmodium berghei and some other intracellular pathogens. | clearance of infection with intracellular pathogens in mice involves interferon-regulated gtpases of the irg protein family. experiments with mice genetically deficient in members of this family such as irgm1(lrg-47), irgm3(igtp), and irgd(irg-47) has revealed a critical role in microbial clearance, especially for toxoplasma gondii. the in vivo role of another member of this family, irga6 (iigp, iigp1) has been studied in less detail. we investigated the susceptibility of two independently gener ... | 2011 | 21698150 |
| [unusual location of a brain abscess due to listeria monocytogenes.] | here we report a case of sustentorial brain abscess due to listeria monocytogenes. blood culture and procalcitonine blood measurement were negative. l. monocytogenes was isolated from csf after inoculation in castañeda medium. | 2011 | 21835558 |
| bacterial extract cantastim activates macrophages via tlr-2. | cantastim is a second generation bacterial immunomodulator. the aim of this study was to examine the mechanism by which bacterial immunomodulator cantastim induces production of inflammatory cytokines in monocytes/macrophages. proinflammatory cytokines were induced in pma-differentiated thp-1 cells by stimulation with tlr agonists and cantastim in the presence or absence of anti-tlr blocking antibodies or isotype matched control antibodies. also, rna interference was used to knockdown tlr2 or tl ... | 2011 | 21717809 |
| the effect of using citric or acetic acid on survival of listeria monocytogenes during fish protein recovery by isoelectric solubilization and precipitation process. | isoelectric solubilization and precipitation (isp) is a protein recovery process effective at reducing listeria innocua, a nonpathogenic bacterium typically used as a surrogate for l. monocytogenes in recovered trout protein. the response of l. monocytogenes to isp processing was determined and compared to the response of l. innocua. headed and gutted rainbow trout were inoculated with l. monocytogenes (10.16 log cfu/g), homogenized, and ph-adjusted with granular citric acid (ph 2.0 and 2.5) or ... | 2011 | 21913922 |
| associations among pathogenic bacteria, parasites, and environmental and land use factors in multiple mixed-use watersheds. | over a five year period (2004-08), 1171 surface water samples were collected from up to 24 sampling locations representing a wide range of stream orders, in a river basin in eastern ontario, canada. water was analyzed for cryptosporidium oocysts and giardia cyst densities, the presence of salmonella enterica subspecies enterica, campylobacter spp., listeria monocytogenes, and escherichia coli o157:h7. the study objective was to explore associations among pathogen densities/occurrence and objecti ... | 2011 | 21889781 |
| the effect of nacl-free processing and high pressure on the fate of listeria monocytogenes and salmonella on sliced smoked dry-cured ham. | nacl is an important multifunctional ingredient applied in dry-cured ham elaboration. however, its excessive intake has been linked to serious cardiovascular diseases causing a recent increase in the development of reduced salt products. in the present study listeria monocytogenes and salmonella, food-borne pathogens which can cross-contaminate post processed products, were spiked with <100 cfu/g on slices of both standard (s) and nacl-free processed (f) (elaborated with kcl+potassium lactate in ... | 2011 | 22000500 |
| behaviour of listeria monocytogenes in raw sausages (merguez) in presence of a bacteriocin-producing lactococcal strain as a protective culture. | the effectiveness of a bacteriocin produced by lactococcus lactis subsp. lactis m in reducing population level and growth of listeria monocytogenes atcc 7644 in fermented merguez sausage was examined. two different formulas (with or without added nitrites) were assayed and predetermined numbers of listeria (ca 10(6) cfu g(-1)) were added to sausage mixture. the effect of in situ production of the bacteriocin by lactococcus lactis m on listeria monocytogenes atcc 7644 during fermentation and stor ... | 2003 | 22062517 |
| molecular tracking of listeria monocytogenes in an iberian pig abattoir and processing plant. | the environment and products from an iberian pig abattoir and processing plant were sampled to investigate the prevalence and genetic diversity of listeria monocytogenes. the organism was not detected in the pig carcasses prior to processing. fresh and dry-cured pork did show detectable levels, always ranging below 100cfu per gram. a total of 163 l. monocytogenes isolates collected during one year were characterized by pcr-based serotyping and pulsed-field gel electrophoresis (pfge) restriction ... | 2008 | 22062102 |
| Listeria monocytogenes cell wall constituents' charge effect on electroporation threshold. | Genetically engineered cells with mutations of relevance to electroporation, cell membrane permeabilization by electric pulses, can become a promising new tool for fundamental research on this important biotechnology. Listeria monocytogenes mutants lacking DltA or MprF and assayed for sensitivity to the cathelicidin like anti-microbial cationic peptide (mCRAMP), were developed to study the effect of cell wall charge on electroporation. Working in the irreversible electroporation regime (IRE), we ... | 2011 | 22100748 |
| influence of rosemary-tocopherol/packaging combination on meat quality and the survival of pathogens in restructured irradiated pork loins. | irradiated restructured pork loins treated with rosemary-tocopherol/double-packaging had lower tbars values than vacuum-packaged control after 10 days of refrigerated storage. the rosemary-tocopherol combination, however, had no effect on the production of sulfur volatiles responsible for the irradiation off-odor, and color changes in irradiated pork. v7/a3 double-packaging was effective in reducing the sulfur volatiles significantly. rosemary-tocopherol combination was highly effective in reduc ... | 2006 | 22062849 |
| purification, crystallization and preliminary x-ray crystallographic analysis of pbp4 from listeria monocytogenes. | penicillin-binding proteins (pbps), which catalyze peptidoglycan synthesis, have been extensively studied as a well established target of antimicrobial agents, including β-lactam derivatives. however, remarkable resistance to β-lactams has developed among pathogenic bacteria since the clinical use of penicillin began. recently, the glycosyltransferase (gt) domain of class a pbps has been proposed as an attractive target for antibiotic development as moenomycin-bound gt-domain structures have bee ... | 2011 | 22102039 |
| HTLV-1 bZIP factor impairs cell-mediated immunity by suppressing production of Th1 cytokines. | Adult T-cell leukemia (ATL) patients and human T-cell leukemia virus-1 (HTLV-1) infected individuals succumb to opportunistic infections. Cell mediated immunity is impaired, yet the mechanism of this impairment has remained elusive. The HTLV-1 basic leucine zipper factor (HBZ) gene is encoded in the minus strand of the viral DNA and is constitutively expressed in infected cells and ATL cells. To test the hypothesis that HBZ contributes to HTLV-1 associated immunodeficiency, we challenged transge ... | 2011 | 22123848 |
| the sensory acceptability of cooked meat products treated with a protective culture depends on glucose content and buffering capacity: a case study with lactobacillus sakei 10a. | biopreservation has been proven to be a promising natural preservation technique, but the impact of protective cultures on the sensory properties of cooked meat products (cmp) is not well documented. this work presents a case study on the protective culture lactobacillus sakei 10a to obtain a clear view on the real consequences of using protective cultures on the sensory quality of cmp. a preliminary screening study on 13 different cmp and more elaborate application trials at 7°c on vacuum packa ... | 2006 | 22063058 |
| Transcytosis of Listeria monocytogenes across the intestinal barrier upon specific targeting of goblet cell accessible E-cadherin. | Listeria monocytogenes (Lm) is a foodborne pathogen that crosses the intestinal barrier upon interaction between its surface protein InlA and its species-specific host receptor E-cadherin (Ecad). Ecad, the key constituent of adherens junctions, is typically situated below tight junctions and therefore considered inaccessible from the intestinal lumen. In this study, we investigated how Lm specifically targets its receptor on intestinal villi and crosses the intestinal epithelium to disseminate s ... | 2011 | 21967767 |
| defining treatment conditions for pulsed electric field pasteurization of apple juice. | the influence of temperature and the presence of n(α)-lauroyl ethylester (ethyl lauroyl arginate, lae) on the inactivation caused by continuous pulsed electric field treatments (pef) in escherichia coli o157:h7 suspended in apple juice have been investigated to define treatment conditions applicable at industrial scale that promote an equivalent safety level when compared with thermal processing. in the range of experimental conditions investigated (outlet temperature: 20-40 °c, electric field s ... | 2011 | 21880388 |
| listeria meningitis and ventriculitis in an immunocompetent child: case report and literature review. | listeria monocytogenes meningitis is very rare in immunocompetent children. we present a case of a previously healthy 6-year-old girl who developed l. monocytogenes meningitis and ventriculitis. we also review the medical literature on non-neonatal l. monocytogenes meningitis in immunocompetent children. | 2011 | 21877182 |
| Listeria monocytogenes inhibition by defatted mustard meal-based edible films. | An antimicrobial edible film was developed from defatted mustard meal (Sinapis alba) (DMM), a byproduct from the bio-fuel industry, without incorporating external antimicrobials and its antimicrobial activity against Listeria monocytogenes and physical properties were investigated. The DMM colloidal solution consisting of 184g water, 14g DMM, and 2g glycerol was homogenized and incubated at 37°C for 0.2, 0.5, 24 or 48h to prepare a film-forming solution. The pH of a portion of the film-forming s ... | 2011 | 22104123 |
| bacterial populations associated with meat from the deboning room of a high throughput red meat abattoir. | developing countries are faced with high incidences of food poisoning outbreaks, with obvious economic consequences. in highly perishable foodstuffs such as fresh red meat the threat of food poisoning is particularly intense. in this study, red meat samples were collected from a deboning room of a high throughput abattoir. the samples were analysed for the presence of bacillus cereus., staphylococcus aureus., pseudomonas spp., listeria monocytogenes., escherichia coli and salmonella spp. the aer ... | 2004 | 22060877 |
| Phage-based biocontrol strategies to reduce foodborne pathogens in foods. | There has been much recent interest in the use of phages as biocontrol agents of foodborne pathogens in animals used for food production, and in the food products themselves. This interest seems to be driven by consumers' request for more natural foods, as well as the fact that foodborne outbreaks continue to occur, globally, in many foods, some of which (such as fresh produce), lack adequate methods to control any pathogenic contamination present. Also, the many successes with respect to regula ... | 2011 | 22164346 |
| recombination and positive selection contributed to the evolution of listeria monocytogenes lineages iii and iv, two distinct and well supported uncommon l. monocytogenes lineages. | listeriamonocytogenes lineages iii and iv represent two uncommon lineages of the human and animal pathogen l. monocytogenes, characterized by occurrence of unusual phenotypic and genetic characteristics that differentiate them from the common lineages i and ii. to gain further insights into the evolution of lineages iii and iv, we amplified and sequenced housekeeping genes (i.e., gap, prs, purm, ribc, and sigb), internalin genes (i.e., inla, inlb, inlc, inlg, inlc2, inld, inle, inlf, and inlh) a ... | 2011 | 21854875 |
| Comparison of automated BAX PCR and standard culture methods for detection of Listeria monocytogenes in blue Crabmeat (Callinectus sapidus) and blue crab processing plants. | This study compared the automated BAX PCR with the standard culture method (SCM) to detect Listeria monocytogenes in blue crab processing plants. Raw crabs, crabmeat, and environmental sponge samples were collected monthly from seven processing plants during the plant operating season, May through November 2006. For detection of L. monocytogenes in raw crabs and crabmeat, enrichment was performed in Listeria enrichment broth, whereas for environmental samples, demi-Fraser broth was used, and th ... | 2011 | 22054196 |
| Reduction of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes with electrolyzed oxidizing water on inoculated hass avocados (Persea americana var. Hass). | This study was intended to evaluate the bactericidal effect of electrolyzed oxidizing water (EOW) and chlorinated water on populations of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes inoculated on avocados (Persea americana var. Hass). In the first experiment, inoculated avocados were treated with a water wash applied by spraying tap water containing 1 mg/liter free chlorine for 15 s (WW); WW treatment and then spraying sodium hypochlorite in water containing 75 mg/l ... | 2011 | 21902927 |
| atypical listeria monocytogenes serotype 4b strains harboring a lineage ii-specific gene cassette. | listeria monocytogenes is the etiological agent of listeriosis, a severe food-borne illness. the population of l. monocytogenes is divided into four lineages (i to iv), and serotype 4b in lineage i has been involved in numerous outbreaks. several serotype 4b epidemic-associated clonal groups (eci, -ii, and -ia) have been identified. in this study, we characterized a panel of strains of serotype 4b that produced atypical results with a serotype-specific multiplex pcr and possessed the lmo0734 to ... | 2011 | 22138999 |
| cloning, expression, and identification of a novel class iia bacteriocin in the escherichia coli cell-free protein expression system. | the nb-c1 gene, acquired from the result of data mining of the lactic acid bacteria genome, is a novel potential class iia bacteriocin gene with the characteristic ygngvxc cluster. to produce soluble nb-c1 efficiently and overcome issues of protein toxicity, we adopted a gfp fusion strategy using an escherichia coli cell-free protein expression system. we constructed the expression vector pivex2.4d-gfp-nb-c1, which was expressed in both the batch mode and the continuous exchange cell-free (cecf) ... | 2011 | 22038547 |
| listeria monocytogenes: a rare cause for an infected abdominal aortic aneurysm. | infected aneurysms associated with listeria monocytogenes are rare. we describe a case of an infrarenal abdominal aortic aneurysm infected by listeria monocytogenes. the aneurysm was diagnosed using a contrast-enhanced computed tomography (ct) scan and the infective organism identified within positive blood cultures. the patient underwent a successful urgent open aortic aneurysm repair and completed a 6-week course of antibiotics. | 2011 | 21937471 |
| two zinc uptake systems contribute to the full virulence of listeria monocytogenes during growth in vitro and in vivo. | we report here the identification and characterization of two zinc uptake systems, zuram and zinabc, in the intracellular pathogen listeria monocytogenes. transcription of both operons was zinc responsive and regulated by the zinc-sensing repressor zur. deletion of either zuram or zina had no detectable effect on growth in defined media, but a double zuram zina mutant was unable to grow in the absence of zinc supplementation. deletion of zina had no detectable effect on intracellular growth in h ... | 2012 | 22025520 |
| hierarchical bayesian models to assess between- and within-batch variability of pathogen contamination in food. | assessing within-batch and between-batch variability is of major interest for risk assessors and risk managers in the context of microbiological contamination of food. for example, the ratio between the within-batch variability and the between-batch variability has a large impact on the results of a sampling plan. here, we designed hierarchical bayesian models to represent such variability. compatible priors were built mathematically to obtain sound model comparisons. a numeric criterion is prop ... | 2011 | 22043854 |
| perforin plays an unexpected role in regulating t-cell contraction during prolonged listeria monocytogenes infection. | after infection or vaccination, antigen-specific t cells proliferate then contract in numbers to a memory set point. t-cell contraction is observed after both acute and prolonged infections although it is unknown if contraction is regulated similarly in both scenarios. here, we show that contraction of antigen-specific cd8(+) and cd4(+) t cells is markedly reduced in tnf/perforin-double deficient (dko) mice responding to attenuated listeria monocytogenes infection. reduced contraction in dko m ... | 2011 | 22161269 |
| Listeria and autophagy escape: Involvement of InlK, an internalin-like protein. | Autophagy is a cell-autonomous mechanism of innate immunity that protects the cytosol against bacterial infection. Invasive bacteria, including Listeria monocytogenes, have thus evolved strategies to counteract a process that limits their intracellular growth. ActA is a surface protein produced by L. monocytogenes to polymerize actin and mediate intra- and intercellular movements, which plays a critical role in autophagy escape. We have recently investigated the role of another L. monocytogenes ... | 2012 | 22082958 |
| phage and their lysins as biocontrol agents for food safety applications. | bacteriophage (phage) are bacterial viruses and are considered to be the most widely distributed and diverse natural biological entities. soon after their discovery, bacteriophage were found to have antimicrobial properties that were exploited in many early anti-infection trials. however, the subsequent discovery of antibiotics led to a decline in the popularity of bacteriophage in much of the western world, although work continued in the former soviet union and eastern europe. as a result of th ... | 2010 | 22129344 |
| Pulsed-field gel electrophoresis (PFGE) analysis of Listeria monocytogenes isolates of clinical, animal, food, and environmental origin from Ireland. | Listeria monocytogenes is an important foodborne human pathogen. Human infection is associated with high mortality rates. Epidemiological investigation and molecular sub-typing can be useful in linking specific sources of infection with human illness. This study describes retrospective use of pulsed-field gel electrophoresis (PFGE) to examine relationships of 222 isolates from human and non-human sources in Ireland. Human clinical isolates from other countries were also examined. Eight small cl ... | 2011 | 22116984 |
| Potential role of chitinases and chitin-binding proteins in host-microbial interactions during the development of intestinal inflammation. | The small and large intestines contain an abundance of luminal antigens derived from food products and enteric microorganisms. The function of intestinal epithelial cells is tightly regulated by several factors produced by enteric bacteria and the epithelial cells themselves. Epithelial cells actively participate in regulating the homeostasis of intestine, and failure of this function leads to abnormal and host-microbial interactions resulting in the development of intestinal inflammation. Major ... | 2011 | 21938682 |
| comparison of two possible routes of pathogen contamination of spinach leaves in a hydroponic cultivation system. | the route of pathogen contamination (from roots versus from leaves) of spinach leaves was investigated with a hydroponic cultivation system. three major bacterial pathogens, escherichia coli o157:h7, salmonella, and listeria monocytogenes, were inoculated into the hydroponic solution, in which the spinach was grown to give concentrations of 10⁶ and 10³ cfu/ml. in parallel, the pathogens were inoculated onto the growing leaf surface by pipetting, to give concentrations of 10⁶ and 10³ cfu per leaf ... | 2011 | 21902924 |
| Genotyping of selected bacterial enteropathogens in Norway. | In Norway the Norwegian Institute of Public Health (NIPH) is the primary facility for nationwide surveillance of foodborne infections, and it is vital that we can perform rapid and high resolution identification of foodborne bacteria at the strain level. During the last decade a rapid introduction of DNA-based methods has been introduced, which show promise in enhancing the speed and discriminatory capability of the typing laboratory. The laboratory responsible for genotyping enteropathogens at ... | 2011 | 21975141 |
| microbiological contamination of raw beef trimmings and ground beef. | to survey the microbiological quality of beef trimmings and final-ground beef, samples were collected from eight commercial grinding facilities, including trimmings from fed-cattle, culled-beef cows, culled-dairy cows, imported-beef trimmings and finished-ground products. trim samples (core and purge) and ground product samples (n=586) were evaluated for aerobic plate (apc), total coliform (tcc), escherichia coli (ecc) and staphylococcus aureus counts and the presence of salmonella spp. and list ... | 2000 | 22061902 |
| statins inhibit inos-mediated microbicidal potential of activated monocyte-derived dendritic cells by an ifn-β-dependent mechanism. | statins are prescribed to 25 million people worldwide for treating hypercholesterolemia and reducing the risk of cardiovascular diseases. however, the side effects of statins on immunity, and particularly on dc immunobiology, have not been analyzed in-depth. here, we have investigated the impact of lovastatin treatment during monocyte differentiation into dcs on the responsiveness of the resulting monocyte-derived dcs (modcs) to tlr-mediated activation. lovastatin positively regulated tlr4 signa ... | 2011 | 21874649 |
| inhibitory effects of uv treatment and a combination of uv and dry heat against pathogens on stainless steel and polypropylene surfaces. | pathogens that contaminate the surfaces of food utensils may contribute to the occurrence of foodborne disease outbreaks. we investigated the efficacy of uv treatment combined with dry heat (50 °c) for inhibiting 5 foodborne pathogens (escherichia coli o157:h7, salmonella typhimurium, pseudomonas aeruginosa, listeria monocytogenes, and staphylococcus aureus) on stainless steel and polypropylene surfaces in this study. we inoculated substrates with each of the 5 foodborne pathogens cultured on ag ... | 2011 | 22132742 |
| A single method for recovery and concentration of enteric viruses and bacteria from fresh-cut vegetables. | Fresh-cut vegetables are prone to be contaminated with foodborne pathogens during growth, harvest, transport and further processing and handling. As most of these products are generally eaten raw or mildly treated, there is an increase in the number of outbreaks caused by viruses and bacteria associated with fresh vegetables. Foodborne pathogens are usually present at very low levels and have to be concentrated (i.e. viruses) or enriched (i.e. bacteria) to enhance their detection. With this aim, ... | 2012 | 22036077 |
| occurrence of escherichia coli o157:h7, listeria monocytogenes, salmonella and campylobacter spp. on beef carcasses in northern ireland. | a survey of beef carcasses was conducted in all 10 european community approved abattoirs in northern ireland to determine the incidence of escherichia coli o157:h7. analyses were based on excised samples of neck meat taken less than 48 h post-kill. overall, 780 carcasses were sampled and all were negative for e. coli o157:h7. a sub-set of samples was analysed for the presence of listeria monocytogenes (n=200), salmonella (n=200) and campylobacter spp.(n=100). l. monocytogenes was not detected bu ... | 2001 | 22062423 |
| Food safety objectives for Listeria monocytogenes in Spanish food sampled in cafeterias and restaurants. | To gain more insight into the context of food safety management by public administrations, food safety objectives must be studied. The Valencian administration quantified the prevalence of Listeria monocytogenes in cafeterias and restaurants in this region of Spain between 2002 and 2010. The results obtained from this survey are presented here for 2,262 samples of fish, salad, egg, cold meat, and mayonnaise dishes. Microbiological criteria defined for L. monocytogenes were used to differentiate ... | 2011 | 21902930 |