| [virulence-associated gene detection and eric-pcr typing of campylobacter jejuni strains isolated from foods in four southern chinese provinces]. | to know food contamination and genetic diversity of campylobacter jejuni in four provinces of south china, and to provide data for c. jejuni-associated foodborne disease prevention and control. | 2014 | 24783850 |
| cerebral trauma, campylobacter jejuni infection, and monosialotetrahexosylganglioside sodium mediated guillain-barré syndrome in a chinese patient: a rare case event. | | 2014 | 24763774 |
| rapid and high-throughput identification of recombinant bacteria with mass spectrometry assay. | to construct a rapid and high-throughput assay for identifying recombinant bacteria based on mass spectrometry. | 2014 | 24758753 |
| protease-resistant single-domain antibodies inhibit campylobacter jejuni motility. | camelid heavy-chain antibody variable domains (vhhs) are emerging as potential antimicrobial reagents. we have engineered a previously isolated vhh (flagv1m), which binds campylobacter jejuni flagella, for greater thermal and proteolytic stability. mutants of flagv1m were obtained from an error-prone polymerase chain reaction library that was panned in the presence of gastrointestinal (gi) proteases. additional flagv1m mutants were obtained through disulfide-bond engineering. each approach produ ... | 2014 | 24742504 |
| c-reactive protein in patients with guillain barré syndrome. | c-reactive protein (crp) is an acute phase reactant, widely used as a biomarker for various infectious and inflammatory conditions. guillain-barrι syndrome (gbs) is an acute, autoimmune, polyradiculoneuropathy, triggered by infectious agents such as campylobacter jejuni. gbs is generally precipitated 1-3 weeks following c. jejuni infection which suggests a humoral immunopathogenic mechanism. | 2014 | 24739831 |
| pathophysiology and diagnosis of guillain-barré syndrome - challenges and needs. | guillain-barré syndrome (gbs) is an autoimmune polyneuropathy which presents with acute onset and rapid progression of flaccid, hyporeflexi quadriparesis. both sensory and autonomic nerve involvement is seen. gbs has various subtypes that vary in their pathophysiology. the pathogenesis involves an immune response triggered by a preceding event which may be an infection, immunisation or surgical procedure. clinical diagnosis has been largely the primary diagnosing criterion for gbs along with ele ... | 2015 | 24731000 |
| food safety in raw milk production: risk factors associated to bacterial dna contamination. | while human illness from milkborne pathogens may be linked to contamination of the product after pasteurization or improper pasteurization, such diseases are usually associated with consumption of raw milk or its by-products. molecular biology tools were applied to investigate contamination by listeria monocytogenes, salmonella spp., some pathogenic strains of escherichia coli, and campylobacter jejuni in 548 raw milk samples from 125 dairy farms established in two regions from southern brazil. ... | 2014 | 24715203 |
| the campylobacter jejuni racrs system regulates fumarate utilization in a low oxygen environment. | the natural environment of the human pathogen campylobacter jejuni is the gastrointestinal tract of warm-blooded animals. in the gut, the availability of oxygen is limited; therefore, less efficient electron acceptors such as nitrate or fumarate are used by c. jejuni. the molecular mechanisms that regulate the activity of the highly branched respiratory chain of c. jejuni are still a mystery mainly because c. jejuni lacks homologues of transcription factors known to regulate energy metabolism in ... | 2015 | 24707969 |
| water quality monitoring and risk assessment by simultaneous multipathogen quantification. | water quality monitoring and microbial risk assessment are important to ensure safe water for drinking, recreational, and agricultural purposes. in this study, we applied a microfluidic quantitative pcr (mfqpcr) approach to simultaneously quantify multiple waterborne pathogens in a natural freshwater lake in hokkaido, japan, from april to november, 2012. tens of thousands of geese stopped over at this lake during their migration in spring and fall. because lake water is used for irrigation of th ... | 2014 | 24702133 |
| acute motor-sensory axonal neuropathy with hyperreflexia in guillain-barré syndrome. | guillain-barré syndrome is an acute inflammatory autoimmune polyradiculoneuritis. progressive motor weakness and areflexia are essential for its diagnosis. hyperreflexia has rarely been reported in the early healing period of guillain-barré syndrome following campylobacter jejuni infection in patients with acute motor axonal neuropathy with antiganglioside antibody positivity. in this study, we report a 12-year-old girl presenting with complaints of inability to walk, numbness in hands and feet, ... | 2015 | 24700665 |
| estimation of infectious risks in residential populations exposed to airborne pathogens during center pivot irrigation of dairy wastewaters. | in the western united states where dairy wastewaters are commonly land applied, there are concerns over individuals being exposed to airborne pathogens. in response, a quantitative microbial risk assessment (qmra) was performed to estimate infectious risks after inhalation exposure of pathogens aerosolized during center pivot irrigation of diluted dairy wastewaters. the dispersion of pathogens (campylobacter jejuni, escherichia coli o157:h7, non-o157 e. coli, listeria monocytogenes, and salmonel ... | 2014 | 24697271 |
| campylobacter infection has different outcomes in fast- and slow-growing broiler chickens. | campylobacter spp. are frequently carried by poultry, but they are not believed to cause significant disease in these animals. modern poultry breeds have been selected to grow rapidly under intensive conditions, but recently, consumers have moved toward purchasing birds produced in higher welfare, free-range or organic systems. birds reared in these systems tend to be a slower growing breed and are fed a different diet. birds reared in such systems are stocked at a lower density compared with th ... | 2013 | 24689180 |
| campylobacter jejuni strain discrimination and temperature-dependent glycome expression profiling by lectin microarray. | gram-negative campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans worldwide and the most frequently identified infectious trigger in patients developing guillain-barré syndrome (gbs). while c. jejuni is pathogenic in humans, it is a commensal in avian hosts. bacterial cell surface carbohydrates are important virulence factors and play roles in adherence, colonisation and infection. the mechanisms leading to infection or persistent colonisation of c. jejuni are not we ... | 2014 | 24680511 |
| genetic diversity and antibiotic resistance profiles of campylobacter jejuni isolates from poultry and humans in turkey. | in this study, the investigation of clonal relations between human and poultry campylobacter jejuni isolates and the determination of susceptibilities of isolates to various antibiotics were aimed. a total of 200 c. jejuni isolates concurrently obtained from 100 chicken carcasses and 100 humans were genotyped by the pulsed-field gel electrophoresis (pfge) and automated repetitive extragenic palindromic pcr (rep-pcr, diversilab system) methods and were tested for their susceptibility to six antib ... | 2014 | 24667316 |
| a combination of mlst and crispr typing reveals dominant campylobacter jejuni types in organically farmed laying hens. | to elucidate the campylobacter jejuni population in organically farmed laying hens in finland, multilocus sequence typing (mlst) was combined with characterization of clustered regularly interspaced short palindromic repeat (crispr) sequences. | 2014 | 24655229 |
| identification of a protein glycosylation operon from campylobacter jejuni jcm 2013 and its heterologous expression in escherichia coli. | campylobacter jejuni is a human enteropathogenic bacterium possessing an n-glycosylation system. in this work, a protein glycosylation (pgl) operon conferring prokaryotic n-glycosylation in c. jejuni jcm 2013 was cloned and identified. fourteen open reading frames (orfs) were found in the pgl operon. the operon organization was similar to that of c. jejuni nctc 11168, with 98% and 99% identities in overall nucleotide sequence and amino acid sequence, respectively. the pgl operon was heterologous ... | 2014 | 24650731 |
| a capsule conjugate vaccine approach to prevent diarrheal disease caused by campylobacter jejuni. | campylobacter jejuni is a major cause of diarrheal disease and results in high levels of morbidity and economic loss in both industrialized and developing regions of the world. to date, prior vaccine approaches have failed to confer protection against this enteric pathogen. key challenges to the development of a practical campylobacter vaccine for human use include a lack of understanding of campylobacter pathogenesis and well-defined immune correlates of protection. with the discovery that c. j ... | 2014 | 24632556 |
| comparison of campylobacter jejuni pulsotypes isolated from humans and poultry in split and dalmatia county, croatia. | consumption of poultry is considered to be an important source of human infection with campylobacter. in the period from 2008 to 2010, 50 isolates of campylobacter jejuni from human faeces were analysed and compared with 61 isolates from poultry by pulsed field gel electrophoresis using smai and kpni. based on the analysis of smai macrorestriction profiles, 86 isolates (77.5 %) were assigned to 15 s clusters: 31 (62 %) from humans and 55 from poultry (90.2 %). altogether 21 isolates (19 %) exhib ... | 2015 | 24624966 |
| antibacterial and antioxidant activities in extracts of fully grown cladodes of 8 cultivars of cactus pear. | the antimicrobial and antioxidant activities of some cultivars of the nopal cactus have not been determined. in this study, 8 cultivars of nopal cacti from mexico were assayed for phenolic content, antioxidant activities, and antimicrobial activities against campylobacter jejuni, vibrio cholera, and clostridium perfringens. plant material was washed, dried, and macerated in methanol. minimum bactericidal concentrations (mbcs) were determined using the broth microdilution method. antioxidant acti ... | 2014 | 24621296 |
| detection and characterization of campylobacter spp. from 40 dairy cattle herds in quebec, canada. | dairy cattle are considered a campylobacter reservoir in the epidemiology of campylobacteriosis. currently, very little data on the prevalence of campylobacter in dairy herds are available in the province of quebec, canada. the objectives of this study were to evaluate the prevalence of campylobacter associated with management practices in 40 dairy cattle herds as well as to characterize the bacterial genetic diversity. fecal samples from 797 lactating cows of 40 dairy farms, water provided to a ... | 2014 | 24617502 |
| characterization and reactivity of broiler chicken sera to selected recombinant campylobacter jejuni chemotactic proteins. | campylobacter jejuni, a gram-negative rod bacterium, is the leading causative agent of human acute bacterial gastroenteritis worldwide. consumption and handling of raw or undercooked poultry are regarded as a major source for human infection. because bacterial chemotaxis guides microorganisms to colonization and invasion in the host cells, proteins involved in chemotactic processes can be novel targets for vaccine development. in this communication, we report amplification, cloning and expressio ... | 2014 | 24609189 |
| effects of commercial marinade seasoning and a natural blend of cultured sugar and vinegar on campylobacter jejuni and salmonella typhimurium and the texture of chicken breasts. | marination using various ingredients has been widely used to improve microbial safety and quality of chicken products at retail markets. the objective of this study was to investigate the effects of commercial marinade seasoning and cultured sugar/vinegar blend on campylobacter jejuni and salmonella typhimurium populations during refrigerated storage. in addition, their effects on the texture of precooked chicken breasts during frozen and refrigerated storage was investigated. chicken breasts in ... | 2014 | 24604867 |
| health risk assessment for splash parks that use rainwater as source water. | in the netherlands, rainwater becomes more and more popular as an economic and environmentally sustainable water source for splash parks, however, the associated public health risk and underlying risk factors are unknown. since splash parks have been associated with outbreaks of infectious diseases, a quantitative microbial risk assessment was performed using legionella pneumophila as a target pathogen to quantify the risk of infection for exposure due to inhalation and campylobacter jejuni for ... | 2014 | 24576701 |
| campylobacter infection in chickens modulates the intestinal epithelial barrier function. | asymptomatic carriage of campylobacter jejuni is highly prevalent in chicken flocks. thus, we investigated whether chronic campylobacter carriage affects chicken intestinal functions despite the absence of clinical symptoms. an experiment was carried out in which commercial chickens were orally infected with c. jejuni (1 × 10(8) cfu/bird) at 14 days of life. changes in ion transport and barrier function were assessed by short-circuit current (i(sc)) and transepithelial ion conductance (g(t)) in ... | 2015 | 24553586 |
| comparative proteomic label-free analysis of campylobacter jejuni nctc 11168 cultured with porcine mucin. | campylobacter jejuni is a major gastrointestinal pathogen in humans. poultry is a primary reservoir for c. jejuni, and c. jejuni appears to be highly adapted to the gastrointestinal tracts of avian species. we determined the protein expression profiles of c. jejuni nctc 11168 cultured in medium containing porcine mucin. differentially expressed proteins in the presence and absence of porcine mucin were identified using the label-free method. we identified 52 proteins with expression that was eit ... | 2014 | 24552179 |
| the evidence for clonal spreading of quinolone resistance with a particular clonal complex of campylobacter jejuni. | campylobacter is the most prevalent cause of bacterial gastroenteritis worldwide and it represents a significant public health risk of increasing severity due to its escalating resistance to clinically important quinolone and macrolide antibiotics. as a zoonotic pathogen campylobacter is transmitted along the food chain and naturally cycles from environmental waters, feedstuff, animals and food to humans. we determined antibiotic resistance profiles, as well as multilocus sequence types and flaa ... | 2014 | 24534165 |
| reduction of microbiological risk in minced meat by a combination of natural antimicrobials. | responsibility for food safety must be taken through the entire food-production chain, to avoid consumer cross-contamination. the antimicrobial activities of an alpinia katsumadai seed extract and epigallocatechin gallate (egcg), and their combination, were evaluated against individual food-borne pathogenic strains of listeria monocytogenes, escherichia coli and campylobacter jejuni, individually and as a cocktail, in chicken-meat juice and sterile minced meat as food models, and in minced meat ... | 2014 | 24532379 |
| structure of an unusual s-adenosylmethionine synthetase from campylobacter jejuni. | s-adenosylmethionine (adomet) participates in a wide range of methylation and other group-transfer reactions and also serves as the precursor for two groups of quorum-sensing molecules that function as regulators of the production of virulence factors in gram-negative bacteria. the synthesis of adomet is catalyzed by adomet synthetases (mats), a ubiquitous family of enzymes found in species ranging from microorganisms to mammals. the adomet synthetase from the bacterium campylobacter jejuni (cjm ... | 2014 | 24531478 |
| use of synthetic genes for cloning, production and functional expression of the bacteriocins enterocin a and bacteriocin e 50-52 by pichia pastoris and kluyveromyces lactis. | the use of synthetic genes may constitute a successful approach for the heterologous production and functional expression of bacterial antimicrobial peptides (bacteriocins) by recombinant yeasts. in this work, synthetic genes with adapted codon usage designed from the mature amino acid sequence of the bacteriocin enterocin a (enta), produced by enterococcus faecium t136, and the mature bacteriocin e 50-52 (bace50-52), produced by e. faecium nrrl b-32746, were synthesized. the synthetic enta and ... | 2014 | 24510220 |
| proteomic identification of immunodominant membrane-related antigens in campylobacter jejuni associated with sheep abortion. | campylobacter jejuni clone sa is the predominant agent inducing sheep abortion and a zoonotic agent causing gastroenteritis in humans in the united states. in an attempt to identify antigens of clone sa that may be useful for vaccine development, immunoproteomic analyses were conducted to characterize the membrane proteome of c. jejuni clone sa. 2-de of c. jejuni membrane-related proteins was followed by immunoblotting analyses using convalescent sera that were derived from ewes naturally infect ... | 2014 | 24487037 |
| synthesis and structure-activity relationship studies of n-terminal analogues of the antimicrobial peptide tridecaptin a(1). | chemical synthesis was used to increase the potency of the antimicrobial lipopeptide tridecaptin a1. lipid tail modification proved to be an ideal platform for synthesizing structurally simpler analogues that are not readily accessible by isolation. the stereochemical elements of the tridecaptin a1 lipid tail are not essential for antimicrobial activity and could be replaced with hydrophobic aliphatic or aromatic groups. some simpler analogues displayed potent antimicrobial activity against gram ... | 2014 | 24479847 |
| characterization and antigenicity of recombinant campylobacter jejuni flagellar capping protein flid. | campylobacter jejuni, a flagellated, spiral-rod, gram-negative bacterium, is the leading pathogen of human acute bacterial gastroenteritis worldwide, and chickens are regarded as a major reservoir of this micro-organism. bacterial flagella, composed of more than 35 proteins, play important roles in colonization and adhesion to the mucosal surface of chicken caeca. in this study, the flagellar capping protein, flid, encoded by the flid gene, from the campylobacter jenuni d1-39 isolate was express ... | 2014 | 24445509 |
| on the fiftieth anniversary. postinfectious irritable bowel syndrome: mechanisms related to pathogens. | gastrointestinal (gi) infections resulting from bacterial, viral, and parasitic pathogens predispose to postinfectious irritable bowel syndrome (pi-ibs) and other functional gi disorders. existing literature supports the role of enterochromaffin cell hyperplasia, serotonin synthesis and reuptake, impaired barrier function, altered immune activation, and potentially mast cell activation in the pathophysiology of pi-ibs. | 2014 | 24438587 |
| a single nucleotide in the promoter region modulates the expression of the β-lactamase oxa-61 in campylobacter jejuni. | despite prevalent β-lactam resistance in campylobacter jejuni, an important zoonotic enteric pathogen, the molecular basis of β-lactamase-mediated β-lactam resistance is still largely unknown. in particular, some c. jejuni strains that carry β-lactamase gene blaoxa-61 (cj0299) are still susceptible to β-lactams with undetected β-lactamase activity, suggesting blaoxa-61 is subjected to regulation. the objective of this study was to determine the regulatory mechanism of β-lactamase in c. jejuni. | 2014 | 24408987 |
| double-locus sequence typing using pora and peb1a for epidemiological studies of campylobacter jejuni. | campylobacter jejuni is the leading cause of foodborne bacterial gastroenteritis worldwide. bacterial typing schemes play an important role in epidemiological investigations to trace the source and route of transmission of the infectious agent by identifying outbreak and differentiating among sporadic infections. in this study, a double-locus sequence typing (dlst) scheme for c. jejuni based on concatenated partial sequences of pora and peb1a genes is proposed. the dlst scheme was validated usin ... | 2014 | 24404778 |
| methyl-accepting chemotaxis proteins 3 and 4 are responsible for campylobacter jejuni chemotaxis and jejuna colonization in mice in response to sodium deoxycholate. | methyl-accepting chemotaxis proteins (mcps), also termed transducer-like proteins (tlps), serve as sensors in bacterial chemotactic signalling, and detect attractants and promote bacterial movement towards suitable sites for colonization. campylobacter jejuni is a leading cause of human enteritis, but the mechanisms responsible for bacterial chemotaxis and early colonization in the jejunum of hosts are poorly understood. in the present study, we identified several types of bile and sodium deoxyc ... | 2014 | 24403598 |
| isolation of campylobacter from human stool samples. | campylobacter is an undetected cause of diarrhoea especially under 5 years of age in most of the countries. isolation of this organism is difficult, expensive and cumbersome. | 2014 | 24399385 |
| prevalence, concentration and genotypes of campylobacter jejuni in faeces from dairy herds managed in farm systems with or without housing. | to determine the faecal excretion of campylobacter jejuni by dairy cows that used housing in combination with outdoor grazing. | 2014 | 24372778 |
| [frequency of antibodies to the recombinant protein p39 of c. jejuni in patients with gastrointestinal disorders and reactive arthritis in poland]. | campylobacterjejuni is has been found to be the leading cause of bacterial gastroenteritis worldwide. clinical manifestation on enterocolitis caused by c. jejuni are diarrhea, fever, abdominal pain and in some patients, fecal blood. after c. jejuni infection, squeals may occur such as reactive arthritis. the aim of the study was to investigate the frequency of antibodies to the recombinant protein p39 sticks c. jejuni in patients with gastrointestinal disorders and reactive arthritis in poland. | 2014 | 25804073 |
| guillain-barré syndrome in asia. | over the past 20 years, the most notable advance in understanding guillain-barré syndrome (gbs) has been the identification of an axonal variant. this advance arose chiefly through studies undertaken in east asian countries and comprised two major aspects: first, the immunopathogenesis of axonal gbs related to anti-ganglioside antibodies and molecular mimicry of campylobacter jejuni; and second, the observation that distinct electrophysiological patterns of axonal gbs existed, reflecting reversi ... | 2014 | 24357682 |
| use of in vivo-induced antigen technology to identify in vivo-expressed genes of campylobacter jejuni during human infection. | campylobacter jejuni is a prevalent foodborne pathogen worldwide. human infection by c. jejuni primarily arises from contaminated poultry meats. genes expressed in vivo may play an important role in the pathogenicity of c. jejuni. we applied an immunoscreening method, in vivo-induced antigen technology (iviat), to identify in vivo-induced genes during human infection by c. jejuni. an inducible expression library of genomic proteins was constructed from sequenced c. jejuni nctc 11168 and was then ... | 2014 | 24346471 |
| [ill after drinking untreated milk; 'die amelander krankheit']. | in 2012 three patients consulted their general practitioner with symptoms of gastro-enteritis with bloody stools. this was caused by drinking untreated milk infected with campylobacter jejuni. another patient developed reactive arthritis. he too had drunk untreated milk that had probably been infected with yersinia enterocolitica. between 1958 and 1995 many german children living in the region of cologne developed gastro-enteritis after holidaying on ameland, one of the dutch islands. this condi ... | 2013 | 24345367 |
| consumption of raw or unpasteurized milk and milk products by pregnant women and children. | sales of raw or unpasteurized milk and milk products are still legal in at least 30 states in the united states. raw milk and milk products from cows, goats, and sheep continue to be a source of bacterial infections attributable to a number of virulent pathogens, including listeria monocytogenes, campylobacter jejuni, salmonella species, brucella species, and escherichia coli o157. these infections can occur in both healthy and immunocompromised individuals, including older adults, infants, youn ... | 2014 | 24344105 |
| a modified molecular beacons-based multiplex real-time pcr assay for simultaneous detection of eight foodborne pathogens in a single reaction and its application. | foodborne disease outbreaks are often caused by one of the major pathogens. early identification of the causal pathogen is crucial for disease control and prevention. we describe a real-time polymerase chain reaction (rtpcr) assay that can identify, in a single reaction, up to eight common foodborne bacterial pathogens, including salmonella enterica subsp. enterica, listeria monocytogenes, escherichia coli o157, vibrio parahaemolyticus, v. vulnificus, campylobacter jejuni, enterobacter sakazakii ... | 2014 | 24328501 |
| semi-automatic selection of summary statistics for abc model choice. | a central statistical goal is to choose between alternative explanatory models of data. in many modern applications, such as population genetics, it is not possible to apply standard methods based on evaluating the likelihood functions of the models, as these are numerically intractable. approximate bayesian computation (abc) is a commonly used alternative for such situations. abc simulates data x for many parameter values under each model, which is compared to the observed data x obs. more weig ... | 2014 | 24323893 |
| [preparation and characterization of monoclonal antibodies against flhf protein of campylobacter jejuni]. | to develop a prokaryotic expression system for campylobacrer jejuni flhf protein and prepare monoclonal antibodies(mab) against this protein. | 2013 | 24321074 |
| notes from the field: recurrent outbreak of campylobacter jejuni infections associated with a raw milk dairy—pennsylvania, april–may 2013. | | 2013 | 24308038 |
| specificity of a udp-galnac pyranose-furanose mutase: a potential therapeutic target for campylobacter jejuni infections. | pyranose-furanose mutases are essential enzymes in the life cycle of a number of microorganisms, but are absent in mammalian systems, and hence represent novel targets for drug development. to date, all such mutases show preferential recognition of a single substrate (e.g., udp-gal). we report here the detailed structural characterization of the first bifunctional pyranose-furanose mutase, which recognizes both udp-gal and udp-galnac. the enzyme under investigation (cjungm) is involved in the bi ... | 2014 | 24302429 |
| campylobacter jejuni prevalence and hygienic quality of retail bovine ground meat in finland. | detection of common genotypes of campylobacter jejuni among finnish human and bovine isolates, suggested that bovines may be a source for zoonotic camp. jejuni infection. in addition, a finnish epidemiological study implied the tasting and eating raw or undercooked beef as risk factors for acquiring campylobacteriosis. we therefore performed a study on the occurrence of camp. jejuni in retail bovine ground meat in helsinki by the use of both cultivation and pcr. during 2011 and 2012, 175 bovine ... | 2014 | 24299275 |
| eucommia ulmoides oliv.: ethnopharmacology, phytochemistry and pharmacology of an important traditional chinese medicine. | eucommia ulmoides oliv. (family eucommiaceae), also known as dù-zhòng (chinese: ), tuchong (in japanese), is the sole species of the genus eucommia. the leaf, stem, and bark as well as staminate flower of eucommia ulmoides have been traditionally used to cure many diseases in china, japan, korea, among others. the aim of this review is to comprehensively outline the botanical description, ethnopharmacology, phytochemistry, biological activities, and toxicology of eucommia ulmoides and to discuss ... | 2014 | 24296089 |
| bile acid-controlled transgene expression in mammalian cells and mice. | in recent years, using trigger-inducible mammalian gene switches to design sophisticated transcription-control networks has become standard practice in synthetic biology. these switches provide unprecedented precision, complexity and reliability when programming novel mammalian cell functions. metabolite-responsive repressors of human-pathogenic bacteria are particularly attractive for use in these orthogonal synthetic mammalian gene switches because the trigger compound sensitivity often matche ... | 2014 | 24280297 |
| are bacteriophage defence and virulence two sides of the same coin in campylobacter jejuni? | the continuous battle for survival in the environment has led to the development or acquisition of sophisticated defence systems in bacteria. these defence systems have contributed to the survival of the bacterial species in the environment for millions of years. some systems appear to have evolved in a number of pathogenic bacteria towards a role in virulence and host immune evasion. recently, different bacterial cell envelope components from diverse bacterial species have been linked not only ... | 2013 | 24256240 |
| design, synthesis, and biological testing of novel naphthoquinones as substrate-based inhibitors of the quinol/fumarate reductase from wolinella succinogenes. | novel naphthoquinones were designed, synthesized, and tested as substrate-based inhibitors against the membrane-embedded protein quinol/fumarate reductase (qfr) from wolinella succinogenes, a target closely related to qfrs from the human pathogens helicobacter pylori and campylobacter jejuni. for a better understanding of the hitherto structurally unexplored substrate binding pocket, a structure-activity relationship (sar) study was carried out. analogues of lawsone (2-hydroxy-1,4-naphthoquinone ... | 2013 | 24251984 |
| campylobacter jejuni meningitis in a neonate: a rare case report. | campylobacter jejuni is a common cause of gastroenteritis especially in childhood. however, extraintestinal manifestations of c. jejuni such as bacteremia, cholecystitis, pancreatitis and osteomyelitis are rare. meningitis in newborns caused by this organism is unusually rare. we report a case of neonatal meningitis in a 15-day old term boy caused by campylobacter jejuni. | 2013 | 24246522 |
| axonal guillain-barré syndrome: concepts and controversies. | acute motor axonal neuropathy (aman) is a pure motor axonal subtype of guillain-barré syndrome (gbs) that was identified in the late 1990s. in asia and central and south america, it is the major subtype of gbs, seen in 30-65% of patients. aman progresses more rapidly and has an earlier peak than demyelinating gbs; tendon reflexes are relatively preserved or even exaggerated, and autonomic dysfunction is rare. one of the main causes is molecular mimicry of human gangliosides by campylobacter jeju ... | 2013 | 24229616 |
| screening of genes expressed in vivo during interaction between chicken and campylobacter jejuni. | chicken are considered as the most important source of human infection by campylobacter jejuni, which primarily arises from contaminated poultry meats. however, the genes expressed in vivo of the interaction between chicken and c. jejuni have not been screened. in this regard, in vivo-induced antigen technology (iviat) was applied to identify expressed genes in vivo during interaction between chicken and c. jejuni, a prevalent foodborne pathogen worldwide. chicken sera were obtained by inoculati ... | 2014 | 24225374 |
| molecular epidemiology of campylobacter jejuni human and chicken isolates from two health units. | a study was conducted over a 2-year period in the perth district and wellington-dufferin-guelph health units in ontario, with an objective of using comparative genomic fingerprinting (cgf) with a 40-gene assay (cgf40) to investigate the association between human cases of campylobacteriosis and spatially and temporally related campylobacter isolates from retail chicken. cgf results were available for isolates from 115 human cases and 718 retail chicken samples. these data were combined with cgf r ... | 2014 | 24219173 |
| four-year monitoring of foodborne pathogens in raw milk sold by vending machines in italy. | prevalence data were collected from official microbiological records monitoring four selected foodborne pathogens (salmonella, listeria monocytogenes, escherichia coli o157:h7, and campylobacter jejuni) in raw milk sold by self-service vending machines in seven italian regions (60,907 samples from 1,239 vending machines) from 2008 to 2011. data from samples analyzed by both culture-based and real-time pcr methods were collected in one region. one hundred raw milk consumers in four regions were i ... | 2013 | 24215694 |
| treatment of guillain-barré syndrome. | guillain-barré syndrome (gbs) is an idiopathic postinfectious immune disease that leads to progressive motor weakness due to damage to the myelin sheath. epidemiological studies have linked gbs to infections from campylobacter jejuni, cytomegalovirus, and other pathogens. the syndrome includes multiple subtypes, with the most common being ascending motor weakness. treatment with intravenous immunoglobulin and plasma exchange reduces the time for recovery to occur, although some remain disabled. | 2013 | 24202119 |
| multistate outbreak of campylobacter jejuni infections associated with undercooked chicken livers--northeastern united states, 2012. | in october 2012 the vermont department of health (vdh) identified three cases of laboratory-confirmed campylobacter jejuni infection in vermont residents; the isolates had indistinguishable pulsed-field gel electrophoresis (pfge) patterns. a query of pulsenet, the national molecular subtyping network for foodborne disease surveillance, led to the identification of one additional case each from new hampshire, new york, and vermont that had been reported in the preceding 6 months. an investigation ... | 2013 | 24196663 |
| chest pain and diarrhea: a case of campylobacter jejuni-associated myocarditis. | diarrhea and chest pain are common symptoms in patients presenting to the emergency department (ed). however, rarely is a relationship between these two symptoms established in a single patient. | 2014 | 24188611 |
| sialylation of campylobacter jejuni endotoxin promotes dendritic cell-mediated b cell responses through cd14-dependent production of ifn-β and tnf-α. | campylobacter jejuni is the most common bacterial cause of human gastroenteritis and often precedes development of guillain-barré syndrome (gbs), a life-threatening paralytic disease. the incorporation of the carbohydrate sialic acid into c. jejuni lipooligosaccharides (los) is associated with increased severity of gastroenteritis and with induction of gbs; however, the underlying mechanisms remain completely unknown. in this study, we demonstrate that sialic acids in c. jejuni endotoxin enhance ... | 2013 | 24166974 |
| an optimized binary typing panel improves the typing capability for campylobacter jejuni. | campylobacter jejuni is a frequent bacterial pathogen causing gastroenteritis worldwide. we report here a mathematically optimized combination of 10 loci selected from 2 previously published binary typing panels. the optimized combination offers advantages of higher differentiation capability, simplicity, cost-effectiveness, and portability for routine surveillance and outbreak investigations of c. jejuni. | 2013 | 24139878 |
| increased incidence of campylobacter jejuni-associated guillain-barré syndromes in the greater paris area. | the role of campylobacter jejuni as the triggering agent of guillain-barré syndrome (gbs) has not been reassessed since the end of the 1990s in france. we report that the number of c. jejuni-related gbs cases increased continuously between 1996 and 2007 in the paris region (mean annual increment: 7%, p = 0·007). | 2014 | 24107359 |
| cow's milk with active immunoglobulins against campylobacter jejuni: effects of temperature on immunoglobulin activity. | adult holstein cows were injected with an antiserum against campylobacter jejuni and immunoglobulin activities in vitro were determined in blood and milk several weeks after injection. the immunoactivity of immunoglobulins in milk was measured by an elisa after different temperature-time treatments (60-91°c and 4-3600 s) at laboratory and pilot-plant scales. kinetic and thermodynamic parameters were determined. | 2014 | 24105722 |
| quantitative assessment of infection risk from exposure to waterborne pathogens in urban floodwater. | flooding and heavy rainfall have been associated with waterborne infectious disease outbreaks, however, it is unclear to which extent they pose a risk for public health. here, risks of infection from exposure to urban floodwater were assessed using quantitative microbial risk assessment (qmra). to that aim, urban floodwaters were sampled in the netherlands during 23 events in 2011 and 2012. the water contained campylobacter jejuni (prevalence 61%, range 14- >10(3) mpn/l), giardia spp. (35%, 0.1- ... | 2014 | 24095592 |
| a boy with iga nephropathy complicated by tubulointerstitial nephritis and uveitis (tinu) syndrome. | abnormal cellular and humoral immunity underlie both immunoglobulin a (iga) nephropathy and tubulointerstitial nephritis and uveitis (tinu) syndrome. we encountered a teenage boy who developed tinu syndrome during the course of iga nephropathy. | 2015 | 24075025 |
| bioengineered 2'-fucosyllactose and 3-fucosyllactose inhibit the adhesion of pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines. | human milk oligosaccharides help to prevent infectious diseases in breastfed infants. larger scale testing, particularly in animal models and human clinical studies, is still limited due to shortened availability of more complex oligosaccharides. the purpose of this study was to evaluate 2'-fucosyllactose (2'-fl) and 3-fucosyllactose (3-fl) synthesized by whole-cell biocatalysis for their biological activity in vitro. therefore, we have tested these oligosaccharides for their inhibitory potentia ... | 2013 | 24074741 |
| geographic setting influences great lakes beach microbiological water quality. | understanding of factors that influence escherichia coli (ec) and enterococci (ent) concentrations, pathogen occurrence, and microbial sources at great lakes beaches comes largely from individual beach studies. using 12 representative beaches, we tested enrichment cultures from 273 beach water and 22 tributary samples for ec, ent, and genes indicating the bacterial pathogens shiga-toxin producing e. coli (stec), shigella spp. , salmonella spp , campylobacter jejuni/coli , and methicillin-resista ... | 2013 | 24073635 |
| mucosal vaccines: where do we stand? | mucosal vaccinology is a relatively young but rapidly expanding discipline. at present the vast majority of vaccines are administered by injection, including vaccines that protect against mucosally acquired pathogens such as influenza virus and human papilloma virus. however, mucosal immune responses are most efficiently induced by the administration of vaccines onto mucosal surfaces. the small number of currently licensed mucosal vaccines have reduced the burden of disease and mortality caused ... | 2013 | 24066889 |
| isolation and characterization of antimicrobial constituents of searsia chirindensis l. (anacardiaceae) leaf extracts. | searsia chirindensis is used in south african traditional medicine for management of bacterial infections such as diarrhoea. aim of the study was to examine the phytochemical composition from the leaves of searsia chirindensis that is responsible for the ethnomedicinal use of this plant. | 2013 | 24060408 |
| [nosocomial diarrhea in intensive care unit: other than clostridium difficile]. | to investigate the incidence and clinical features of non-clostridium difficile (c. difficile) associated nosocomial diarrhea in intensive care unit (icu) caused by klebsiella oxytoca and clostridium perfringens. | 2013 | 24059125 |
| the globins of campylobacter jejuni. | campylobacter jejuni is a zoonotic gram-negative bacterial pathogen that is exposed to reactive nitrogen species, such as nitric oxide, from a variety of sources. to combat the toxic effects of this nitrosative stress, c. jejuni upregulates a small regulon under the control of the transcriptional activator nssr, which positively regulates the expression of a single-domain globin protein (cgb) and a truncated globin protein (ctb). cgb has previously been shown to detoxify nitric oxide, but the ro ... | 2013 | 24054796 |
| campylobacter jejuni and cytopenias. | leukopenia and thrombocytopenia in a febrile patient are not uncommon and may be a diagnostic clue in patients without an alternative explanation for cytopenias. this has not been reported in campylobacter jejuni infections. | 2013 | 24034076 |
| effect of ciprofloxacin exposure on dna repair mechanisms in campylobacter jejuni. | ciprofloxacin resistance is common both among animal and human campylobacter jejuni isolates. resistant isolates are shown to persist even without selection pressure. to obtain further insight on effects of ciprofloxacin exposure on c. jejuni we compared transcriptional responses of both c. jejuni wild-type strain 81-176 (ciprofloxacin mic 0.125 mg l(-1)) and its intermediate ciprofloxacin-resistant variant p3 (asp90→asn in gyra) in the absence and presence of ciprofloxacin. further, we sequence ... | 2013 | 24025607 |
| safety of long-term ppi therapy. | proton pump inhibitors have become the mainstay of medical treatment of acid-related disorders. long-term use is becoming increasingly common, in some cases without a proper indication. a large number of mainly observational studies on a very wide range of possible associations have been published in the past decade and are critically reviewed in this article and the existing evidence is evaluated and translated into possible clinical consequences. based on the existing evidence the benefits of ... | 2013 | 23998981 |
| recurrent outbreak of campylobacter jejuni infections associated with a raw milk dairy--pennsylvania, april-may 2013. | during may 2013, the pennsylvania department of health investigated an outbreak of campylobacteriosis among consumers of raw (unpasteurized) milk from a dairy certified by the pennsylvania department of agriculture (pda) to sell raw milk onsite, at retail stores, and at off-farm pick-up sites. investigation by the pennsylvania department of health and pda identified six confirmed and two probable cases of campylobacteriosis associated with raw milk from the dairy. a confirmed case was defined as ... | 2013 | 23985499 |
| influence of mutation in cj0183 and cj0588 genes for colonization abilities of campylobacter jejuni in caco-2 cells using confocal laser scanning microscope. | the cj0183 and cj0588 genes identified in the campylobacter jejuni nctc 11168 genome encode proteins with homology to virulence factors found in other bacteria. previous studies showed that single mutation in the cj0183 gene does not affect adhesion of c. jejuni to the caco-2 cell line whereas protein encoded by cj0588 is involved in adherence to the caco-2 cells. in the presented study differences in invasion index were observed between mutants in both genes and single mutation of cj0588 in 811 ... | 2013 | 23971210 |
| campylobacteriosis in returning travellers and potential secondary transmission of exotic strains. | multilocus sequence types (sts) were determined for 232 and 737 campylobacter jejuni/coli isolates from dutch travellers and domestically acquired cases, respectively. putative risk factors for travel-related campylobacteriosis, and for domestically acquired campylobacteriosis caused by exotic sts (putatively carried by returning travellers), were investigated. travelling to asia, africa, latin america and the caribbean, and southern europe significantly increased the risk of acquiring campyloba ... | 2014 | 23962634 |
| [screening and identification of glycoproteins from campylobacter jejuni nctc 11168 based on specific affinity between lectins and glycoproteins]. | to screen glycoproteins from campylobacter jejuni nctc 11168. | 2013 | 23957150 |
| campylobacter infection associated with consumption of duck liver pâté: a retrospective cohort study in the setting of near universal exposure. | a retrospective cohort study was performed following several reported cases of gastrointestinal illness after a catered event. the attack rate was 45/77 (58·4%) by clinical case definition, with four individuals confirmed to have campylobacter. there was near universal exposure to most foodstuffs served; consumption of duck liver pâté [relative risk (rr) 2·53, 95% confidence interval (ci) 1·05-6·10], mixed leaf salad (rr 2·91, 95% ci 1·22-6·92) and table water (rr undefined, p < 0·01) were assoc ... | 2014 | 23953682 |
| influence of acid-adaptation of campylobacter jejuni on adhesion and invasion of int 407 cells. | the aim of this study was to determine the influence of acid-adaptation on the survival as well as adhesion and invasion of human intestinal cells by nine campylobacter jejuni strains after exposure to different stress conditions. acid-adapted and nonadapted c. jejuni were exposed to different secondary stress conditions such as acid (ph 4.5), starvation (phosphate-buffered saline, ph 7.2), or salt (3% wt/vol nacl). after exposure to the secondary stress, the adhesion and invasion abilities of t ... | 2013 | 23952474 |
| the guillain-barré syndrome. | the latest estimation for the frequency of guillain-barré syndrome (gbs) is 1.1 to 1.8 per 100000 persons per year. guillain-barré syndrome is today divided into two major subtypes: acute inflammatory demyelinating polyneuropathy (aidp) and the axonal subtypes, acute motor axonal neuropathy (aman) and acute motor and sensory axonal neuropathy (amsan). the axonal forms of gbs are caused by certain autoimmune mechanisms, due to a molecular mimicry between antecedent bacterial infection (particular ... | 2013 | 23931791 |
| epidemiological investigations on the possible risk of distribution of zoonotic bacteria through apparently healthy homing pigeons. | clinically healthy homing pigeons may serve as an unnoticed reservoir for zoonotic bacteria. hence, healthy pigeons from 172 different racing pigeon lofts were examined for salmonella serovars, campylobacter spp. and chlamydophila (chlamydia) psittaci. two samplings were performed during the racing season in summer (1242 adult and 1164 juvenile pigeons) and two during winter (1074 adult pigeons). each sampling was accompanied by a questionnaire to identify risk factors for positive lofts. betwee ... | 2013 | 23930968 |
| community-acquired infectious diarrhoea in children under 5 years of age in dakar, senegal. | in sub-saharan africa, infectious diarrhoea is a major cause of childhood morbidity and mortality. a cross-sectional study was undertaken to document the pathogens potentially involved in community-acquired childhood diarrhoea in dakar, the capital of senegal. | 2013 | 23930725 |
| binational outbreak of guillain-barré syndrome associated with campylobacter jejuni infection, mexico and usa, 2011. | in june 2011, a cluster of suspected cases of guillain-barré syndrome (gbs), which can follow campylobacter jejuni infection, was identified in san luis río colorado (slrc), sonora, mexico and yuma county, arizona, usa. an outbreak investigation identified 26 patients (18 from sonora, eight from arizona) with onset of gbs 4 may-21 july 2011, exceeding the expected number of cases (n = 1-2). twenty-one (81%) patients reported antecedent diarrhoea, and 61% of 18 patients tested were seropositive f ... | 2014 | 23924442 |
| tetrazolium reduction allows assessment of biofilm formation by campylobacter jejuni in a food matrix model. | to develop a staining method for specific detection of metabolically active (viable) cells in biofilms of the foodborne pathogen campylobacter jejuni. | 2013 | 23910098 |
| development of a magnetic nanoparticles microarray for simultaneous and simple detection of foodborne pathogens. | foodborne diseases are a widespread and growing public health problem affecting both developed and developing countries, microbiologically contaminated food and water are the major causes of diarrhoeal diseases. methods based on polymerase chain reaction (pcr) and microarrays are rapid and sensitive enough to detect very small quantities of microorganisms, however, the requirement for expensive equipments limits their application. in the present paper, we describe a method based on multiplex pcr ... | 2013 | 23909141 |
| dihydrodipicolinate synthase from campylobacter jejuni: kinetic mechanism of cooperative allosteric inhibition and inhibitor-induced substrate cooperativity. | dihydrodipicolinate synthase (dhdps), an enzyme of the meso-diaminopimelate pathway of lysine biosynthesis, is essential for bacterial growth and is considered a target for novel antibiotics. we have studied dhdps from campylobacter jejuni for the first time, determining the kinetic mechanism of catalysis and inhibition with its natural allosteric feedback inhibitor (s)-lysine. the tetrameric enzyme is known to have two allosteric sites, each of which binds two molecules of lysine. the results s ... | 2013 | 23902434 |
| quantum dot-based immunochromatography test strip for rapid detection of campylobacter jejuni. | campylobacter jejuni is a recognized human foodborne pathogen which is one of the leading causes of human gastrointestinal enteritis worldwide. in stress conditions, c. jejuni is able to enter into a viable but non-culturable state that is difficult to diagnose. hence a rapid, sensitive, and specific method is required to monitor food and water for natural or intentional contamination by this pathogen. we report a quantum dot (qd)-based immunochromatography test strip (qdits) for rapid detection ... | 2013 | 23901473 |
| infectious and noninfectious triggers in guillain-barré syndrome. | guillain-barré syndrome (gbs) is the commonest cause of acquired flaccid paralysis in the world and regarded by many as the prototype for postinfectious autoimmunity. here the authors consider both infectious and noninfectious triggers of gbs and determine where possible what immunological mechanisms may account for this association. in approximately two-thirds of cases, an infectious trigger is reported in the weeks that lead up to disease onset, indicating that the host's response to infection ... | 2013 | 23899233 |
| phage receptor binding protein-based magnetic enrichment method as an aid for real time pcr detection of foodborne bacteria. | we present a novel phage receptor binding protein-based magnetic separation and pre-enrichment method as an alternative to the immunomagnetic separation methods by replacing antibodies with bacteriophage receptor binding proteins (rbps). we couple the proposed rbp-based magnetic separation with real time pcr for rapid, sensitive and specific detection of campylobacter jejuni cells in artificially contaminated skim milk, milk with 2% fat and chicken broth. recovery rates, assessed by real time pc ... | 2013 | 23897488 |
| engineered genetic selection links in vivo protein folding and stability with asparagine-linked glycosylation. | predicting the structural consequences of site-specific glycosylation remains a major challenge due in part to the lack of convenient experimental tools for rapidly determining how glycosylation influences protein folding. to address this shortcoming, we developed a genetic selection that directly links the in vivo folding of asparagine-linked (n-linked) glycoproteins with antibiotic resistance. using this assay, we identified three known or putative glycoproteins from campylobacter jejuni (peb3 ... | 2013 | 23894044 |
| evaluation of nanoparticle-encapsulated outer membrane proteins for the control of campylobacter jejuni colonization in chickens. | numerous vaccination strategies have been evaluated to develop effective vaccines against campylobacter jejuni colonization in poultry but with limited success. the following experiments were conducted to investigate the effect of biodegradable and biocompatible poly (lactide-co-glycolide) nanoparticle (np) encapsulated outer membrane proteins (omp) of c. jejuni. chickens were vaccinated with different routes [subcutaneous (s/c) or oral] and doses (25, 125, or 250 µg) of candidate nanoparticle v ... | 2013 | 23873570 |
| molecular methods to investigate adhesion, transmigration, invasion and intracellular survival of the foodborne pathogen campylobacter jejuni. | campylobacter jejuni is a spiral-shaped gram-negative pathogen and major agent of gastrointestinal foodborne illness in humans worldwide. this pathogen encodes numerous described pathogenicity-associated factors involved in important processes including bacterial adhesion to, transmigration across, invasion into and intracellular survival within intestinal epithelial cells. this review article highlights various molecular techniques applied in the studies of each of these individual steps of c. ... | 2013 | 23872466 |
| is clostridium difficile associated with relapse of inflammatory bowel disease? results from a retrospective and prospective cohort study in the netherlands. | although clostridium difficile may be associated with exacerbations in inflammatory bowel diseases (ibd), prospective studies identifying the role of c. difficile in disease activity are currently lacking. we examined the prevalence of c. difficile in feces of (1) symptomatic ibd patients retrospectively and (2) consecutive outpatients in relation to disease activity prospectively. | 2013 | 23867869 |
| anti-ganglioside antibodies in patients with guillain barré syndrome and other neurological disorders. | a study was performed on 59 guillain-barré syndrome (gbs) cases, 58 neurological controls (nc) and 60 non-neurological controls (nnc) to investigate the association of anti-ganglioside antibodies in gbs and other neurological disorders. campylobacter jejuni was isolated from 5.7% of gbs patients. anti-ganglioside immunoglobulin g was present in 82% and immunoglobulin m in 46% in acute inflammatory demyelinating polyneuropathy patients, 70% and 44% respectively in acute motor axonal neuropathy su ... | 2013 | 23867676 |
| the xer/dif site-specific recombination system of campylobacter jejuni. | chromosome dimers, which form during the bacterial life cycle, represent a problem that must be solved by the bacterial cell machinery so that chromosome segregation can occur effectively. the xer/dif site-specific recombination system, utilized by most bacteria, resolves chromosome dimers into monomers using two tyrosine recombinases, xerc and xerd, to perform the recombination reaction at the dif site which consists of 28-30 bp. however, single xer recombinase systems have been recently discov ... | 2013 | 23861023 |
| a novel immunoproteomics method for identifying in vivo-induced campylobacter jejuni antigens using pre-adsorbed sera from infected patients. | campylobacter jejuni is an important food-borne and zoonotic pathogen with a worldwide distribution. humans and chickens are hosts of this pathogen. at present, there is no ideal vaccine for controlling human campylobacteriosis or the carriage of c. jejuni by chickens. bacterial in vivo-induced antigens are useful as potential vaccine candidates and biomarkers of virulence. | 2013 | 23850640 |
| dynamics of the cag-type iv secretion system of helicobacter pylori as studied by bacterial co-infections. | many pathogenic gram-negative bacteria possess type iv secretion systems (t4ss) to inject effector proteins directly into host cells to modulate cellular processes to their benefit. the human bacterial pathogen helicobacter pylori, a major aetiological agent in the development of chronic gastritis, duodenal ulcer and gastric carcinoma, harbours the cag-t4ss to inject the cytotoxin associated antigen (caga) into gastric epithelial cells. this results in deregulation of major signalling cascades, ... | 2013 | 23844976 |