| antigens and allergens of aspergillus fumigatus. ii. their further identification and partial characterization of a major allergen (ag 3). | these further studies demonstrated that most antigens and allergens detected by allergic bronchopulmonary aspergillosis (abpa) sera were recognized by rabbit antiserum, and a major allergenic component, ag 3, as previously demonstrated by self-crossed radioimmunoelectrophoresis, was identified in the conventional system. affinocrossed immunoelectrophoresis with concanavalin a indicated that for the patients with abpa, although the major antigens contained alpha-d-manno- or alpha-d-glucopyranosid ... | 1986 | 3522706 |
| antigens and allergens of aspergillus fumigatus. i. characterization by quantitative immunoelectrophoretic techniques. | both the igg (precipitinogen) and ige combining components of aspergillus fumigatus have been studied by quantitative immunoelectrophoretic techniques with sera of patients with allergic bronchopulmonary aspergillosis and specific rabbit antisera. considerable batch-to-batch variation in biochemical properties and in immunologic (both antigenic and allergenic) activity was revealed in identically prepared culture-filtrate antigens of the same strain of a. fumigatus. profiles of crossed immunoele ... | 1986 | 3522708 |
| serum iga antibodies to aspergillus fumigatus in various stages of allergic bronchopulmonary aspergillosis. | with double antibody elisa, serum iga antibodies against aspergillus fumigatus (af) were measured from patients in stages i through v allergic bronchopulmonary aspergillosis (abpa). all sera from patients with abpa demonstrated considerably greater values for iga-af than sera from nonatopic subjects. when this sera was compared with sera from patients with asthma and immediate cutaneous reactivity to aspergillus, the present studies documented markedly elevated serum iga-af in the acute, exacerb ... | 1986 | 3522709 |
| treatment of canine nasal aspergillosis with systemic ketoconazole and topical enilconazole. | | 1986 | 3523960 |
| [mycotic lesions of the bronchopulmonary system (experimental study)]. | | 1986 | 3525333 |
| in vitro killing of spores and hyphae of aspergillus fumigatus and rhizopus oryzae by rabbit neutrophil cationic peptides and bronchoalveolar macrophages. | the fungicidal activity of six rabbit neutrophil cationic peptides (np) against resting (dormant) spores, preincubated (swollen) spores, and hyphae of aspergillus fumigatus and rhizopus oryzae was examined. whereas resting spores of both fungi were totally resistant to killing by up to 500 micrograms of np/ml, preincubated spores and hyphae were sensitive, under optimal conditions, to less than 1 microgram /ml. np-1 and np-2, also found in rabbit bronchoalveolar macrophages, were the most active ... | 1986 | 3525696 |
| aspergillus lung disease. | | 1986 | 3525822 |
| [chronic necrotizing pulmonary aspergillosis]. | | 1986 | 3526022 |
| [empyema caused by aspergillus. apropos of 3 cases]. | | 1986 | 3526429 |
| fatal aspergillus pneumonia in chronic granulomatous disease. | a 20-year-old man died from aspergillus pneumonia three weeks after heavy exposure to grain dust. lung biopsy and autopsy demonstrated a distinctive form of suppurating granulomatous bronchopneumonia caused by aspergillus fumigatus, which was the sole agent cultured from the tissue. the liver and lymph nodes contained pigmented lipid histiocytes characteristic of chronic granulomatous disease, and subsequently both of the patient's brothers were found to have x-linked chronic granulomatous disea ... | 1986 | 3526863 |
| development of a sandwich elisa to detect igg and igg sub-class antibodies specific for a major antigen (ag 7) of aspergillus fumigatus. | a sandwich elisa has been developed, using an affinity purified monospecific antiserum as a capture antibody, to detect specific igg and igg sub-classes to a major antigen (ag 7) of aspergillus fumigatus in the sera of patients with allergic bronchopulmonary aspergillosis (abpa). significantly elevated levels of specific igg to ag 7 were detected in 97% of abpa sera tested, as compared to control sera and to sera from a. fumigatus skin-prick test positive individuals. igg sub-class antibody leve ... | 1986 | 3527473 |
| primary pulmonary aspergilloma: case report and review of the literature. | we describe a patient with acute leukemia who developed a primary aspergilloma. a combination of surgical resection and parenteral amphotericin b cured the lesion. the pathophysiology, diagnosis and treatment of primary pulmonary aspergilloma are reviewed. | 1986 | 3528050 |
| [mycetoma of the lungs]. | mycetomas of the lungs are, as a rule, localized in the upper (62), medial (22) and very rarely (8) in the lower portions of the lungs. most frequent localization is the right lung. operations were fulfilled on 68 of 92 patients. surgery was shown to be the most effective method of treatment of patients with lung mycetomas. it results in complete and clinical recovery. | 1986 | 3529583 |
| aspergillosis--clinical aspects. | allergic bronchopulmonary aspergillosis (abpa) is much more common than originally suspected, can have its onset in childhood and remain undiagnosed for years or decades, at which time it presents in a patient with end-stage fibrotic lung disease. in other patients, abpa may cause a finite number of roentgenographic lesions and not be associated with chronic sputum production or widespread bronchiectasis. clinical symptoms range from the patient being asymptomatic with a new roentgenographic inf ... | 1986 | 3532632 |
| aspergillus fumigatus antigens used in the serodiagnosis of aspergillosis. | the diagnostic antigens in aspergillus serology are mycelial or culture filtrate concentrates. recent work has been directed towards the separation of defined, reproducible products of greater sensitivity and specificity than crude extracts. conventional methods of separation have been used; they include fractional precipitation, detergent extraction, chromatography, and preparative isoelectric focusing. selective staining of the separated fractions has shown many of the more reactive components ... | 1986 | 3532633 |
| allergens of aspergillus fumigatus: identification and characterization of components combining with specific ige antibody. | aspergillus fumigatus-specific ige antibodies, mediating immediate type hypersensitivity reactions are found in a proportion (16-26%) of patients with asthma and their presence is a criterion for diagnosis of allergic bronchopulmonary aspergillosis (abpa). sensitive radiolabelled or enzyme-linked immunoassays are required for detection/quantification of these antibodies in patient's serum, and the fungal components, allergens, to which they combine, may be identified in crossed radio-immunoelect ... | 1986 | 3532634 |
| recent statistical survey of visceral aspergillosis in japan, and experimental studies on the pathogenicity of aspergillus fumigatus in rabbits. | a statistical survey of visceral aspergillosis in japanese pathological autopsies (1972-1981) was carried out. it was found that the infection has increased remarkably in recent years, and the results obtained were briefly described. pathogenicity of several strains of aspergillus fumigatus in rabbits was studied on three occasions in the past 31 years. the pathogenicity appeared to be unchanged even after long subcultures for 13 years, while lowered pathogenicity was noted with long-term subcul ... | 1986 | 3532636 |
| cerebral aspergillosis and encephalomeningocele. | a case of massive cerebral aspergillosis complicating a frontal encephalomeningocele is reported. the etiology and pathogenesis are discussed and the diagnosis, symptoms, and treatment possibilities reviewed. | 1986 | 3536091 |
| [clinical course and diagnosis of chronic aspergillosis of the bronchi and lungs]. | | 1986 | 3536674 |
| inhibition of complement by culture supernatants of aspergillus fumigatus. | aspergillus fumigatus produced a water-soluble extracellular material that inhibited the opsonization of fungal cells by normal human serum. blastospores of cryptococcus neoformans and conidia from a. fumigatus or aspergillus niger were used as fungal targets for ingestion by human monocytes. the opsonic inhibitor from a. fumigatus was found to decrease binding of complement component c3b to fungal surfaces by selectively interfering with activation of the alternative complement pathway. inhibit ... | 1986 | 3537152 |
| successful management of primary invasive pulmonary aspergillosis. | we present the successful recovery of an immunocompetent patient with invasive pulmonary aspergillosis in the absence of any previous detectable lung damage or underlying systemic disease. | 1986 | 3539169 |
| characterization of a major antigenic component of aspergillus fumigatus. | a component of aspergillus fumigatus, ag 7, previously identified as a major antigen for patients with allergic bronchopulmonary aspergillosis, has been isolated by gel filtration and further purified by affinity chromatography using monospecific antiserum. the antigen, which binds both specific igg and ige antibodies, was shown to be a high molecular weight, 150-200 kd, heat-stable glycoprotein, which binds to concanavalin a, suggesting the presence of alpha-d mannopyrannoside, or alpha-d gluco ... | 1986 | 3539424 |
| in-hospital source of airborne penicillium species spores. | between 16 july and 1 october 1984, prospectively monitored corridor air samples from a bone marrow transplant station revealed a marked increase in airborne thermotolerant penicillium spores. simultaneous cultures of outside air showed lower spore counts, which were unchanged before, during, and after the corridor outburst, establishing that the source was within the hospital. although the corridor was equipped with recirculating high-efficiency particulate air filtration units which provided 1 ... | 1987 | 3539981 |
| aspergillus fumigatus-specific antibodies in allergic bronchopulmonary aspergillosis and aspergilloma: evidence for a polyclonal antibody response. | patients with the aspergillus-induced diseases allergic bronchopulmonary aspergillosis (abpa), aspergilloma (fungus ball), and aspergillus skin test-positive asthma were differentiated immunologically by radioimmunoassay based on their total immunoglobulin e (ige) and aspergillus fumigatus-specific ige levels. in this study, a new, highly sensitive biotin-avidin-linked immunosorbent assay was used to evaluate a. fumigatus-specific antibodies of all immunoglobulin classes. studied populations inc ... | 1987 | 3539998 |
| serodiagnosis of invasive aspergillosis in patients with hematologic malignancy: validation of the aspergillus fumigatus antigen radioimmunoassay. | six hundred sixteen sera from 79 hematology patients admitted on 152 occasions were analyzed for validation of the aspergillus fumigatus antigen radioimmunoassay (ria). invasive aspergillosis developed on 24 admissions of 22 patients. maximal antigenic activity was significantly higher in patients with invasive aspergillosis than in controls (p less than .0005). at the level of antigenic activity selected as the cutoff value, the sensitivity of the ria was 74%, the specificity 90%, the positive ... | 1987 | 3540137 |
| [aspergillus pulmonary infection. diagnosis using transthoracic aspiration puncture]. | | 1986 | 3540481 |
| transepithelial elimination in cutaneous aspergillosis. | | 1986 | 3543075 |
| computed tomography of renal aspergillosis. | the ct and sonographic findings of a case of localized, invasive aspergillosis of the kidney are presented. | 1987 | 3543078 |
| [two resected cases of pulmonary aspergillosis coincidentally accompanied by a solid tumor]. | | 1986 | 3543449 |
| stage v (fibrotic) allergic bronchopulmonary aspergillosis. a review of 17 cases followed from diagnosis. | a review of the records of 17 patients with stage v (fibrotic stage) allergic bronchopulmonary aspergillosis observed since initial diagnosis (mean observation period, 4.9 years) demonstrated that, of the 11 surviving patients, four have very severe respiratory impairment. the other seven patients have mild or moderate functional impairment, but most of these have not shown clinical deterioration during the observation period. the occurrence of new roentgenographic infiltrates after the time of ... | 1987 | 3545117 |
| synthesis and quantitative structure-activity relationship analysis of n-triiodoallyl- and n-iodopropargylazoles. new antifungal agents. | new series of n-(2,3,3-triiodoallyl) and n-(3-iodopropargyl) azole derivatives (100 compounds) involving pyrrole, pyrazole, imidazole, triazole, and tetrazole nuclei were synthesized successively with the aid of quantitative structure-activity relationship (qsar) analysis to obtain potent antifungal agents. starting from the derivatives of nitropyrrole-containing antibiotics, the qsar analysis of the pyrrole derivatives against candida albicans and trichophyton mentagrophytes strains indicated t ... | 1987 | 3546691 |
| cross-reactivity between antigens of fungal extracts studied by rast inhibition and immunoblot technique. | we have used the rast-inhibition technique in homologous and heterologous inhibitions of the aspergillus fumigatus, alternaria tenuis, and cladosporium herbarum rast assays followed by the immunoblot technique to assess the degree of shared allergenic determinants in these extracts. in the aspergillus rast, there was little or no inhibition with cladosporium and alternaria, but considerable cross-reactivity was found between alternaria and cladosporium. inhibition by alternaria of the cladospori ... | 1987 | 3549841 |
| receptor-mediated clearance of aspergillus galactomannan. | the fate of radiolabeled aspergillus fumigatus galactomannan was studied after intravenous injection into rabbits and rats. at 1 hr, the liver contained 35% of the injected dose in rabbits and 30% in rats. excretion of galactomannan into the urine, measured in rabbits, was another major catabolic route and accounted for 35% of the dose by 24 hr. immunization of rabbits increased hepatic uptake and decreased urinary excretion. hepatic uptake in unimmunized rats could be decreased by saccharomyces ... | 1987 | 3549914 |
| histopathology of organ lesions in mice after an intravenous or intratracheal or intrarenal infection with aspergillus fumigatus. | | 1987 | 3550456 |
| tremorgenic mycotoxins from aspergillus fumigatus as a possible occupational health problem in sawmills. | wood-trimmers' disease, generally called extrinsic allergic alveolitis, which affects workers in sawmills, is thought to be caused by fungal diaspores. the importance of aspergillus fumigatus on the surface of wood dried in kilns is accentuated by its ability to produce tremorgenic mycotoxins. eight strains of a. fumigatus from five different sawmills were isolated and cultivated on liquid media, and one of the strains was also cultivated on wood blocks. extracts were prepared, and the tremorgen ... | 1987 | 3555338 |
| microbiological diagnosis of suppurative keratitis in bangladesh. | experience in setting up an inexpensive microbiology laboratory in the bangladesh national society for the blind eye hospital and training complex at chittagong is presented, together with the results of a pilot study to identify organisms responsible in 33 consecutive cases of suppurative keratitis in the chittagong area of bangladesh. of the 33 cases 21 were positively identified by means of gram stain and/or culture. two-thirds of the responsible organisms were bacteria, and one-third were fu ... | 1987 | 3555609 |
| the role of infection in asthma. | the possible role of bacterial, viral and fungal infections in the development, exacerbation and treatment of asthma are discussed. although bacterial allergy has in the past been advocated as an important etiologic factor for asthma, the evidence is inconclusive. hyposensitization with bacterial antigens is no longer an accepted treatment. bacterial infection of the nasal sinuses and bronchi may exacerbate an asthmatic attack and in some cases patients benefit from antibiotic therapy. however, ... | 1987 | 3556065 |
| allergic bronchopulmonary aspergillosis. model of bronchopulmonary disease with defined serologic, radiologic, pathologic and clinical findings from asthma to fatal destructive lung disease. | allergic bronchopulmonary aspergillosis (abpa) complicates asthma and results in immunologic lung destruction. respiratory failure or fatalities from end-stage fibrotic lung disease have occurred in patients in the third and fourth decades of life. allergic bronchopulmonary aspergillosis may be confirmed in patients with varying severity of asthma from minimal to corticosteroid-dependent and has been reported to occur in approximately 10 percent of patients with cystic fibrosis. it has been docu ... | 1987 | 3556066 |
| aspergillus myositis in a patient with a myelodysplastic syndrome. | we present the case of an elderly man who, while being treated with corticosteroids for a myelodysplastic syndrome, developed myositis of the calf due to aspergillus fumigatus. despite therapy with amphotericin b the myositis failed to resolve and he died. at autopsy, a localized necrotizing myositis of the right calf was found with no evidence of disseminated aspergillus infection. myositis in the setting of disseminated candidiasis or cryptococcosis has been previously reported. this case is u ... | 1987 | 3574431 |
| direct examination vs culture of biopsy specimens for the diagnosis of keratomycosis. | in two patients with fungal keratitis, direct examination of corneal biopsy specimens showed positive fungal elements, but cultures of biopsy specimens failed to disclose fungal growth. we compared the value of direct examination and culture of biopsy specimens in the diagnosis of keratomycosis in rabbits with experimental fungal keratitis caused by fusarium solani, aspergillus fumigatus, and candida albicans. cultures disclosed seven specimens (70%) positive for candida and eight (80%) for fusa ... | 1987 | 3578461 |
| separation of organic dust from microorganism suspensions by partitioning in aqueous polymer two-phase systems. | conidia of penicillium brevi-compactum and aspergillus fumigatus, sporangiospores of rhizopus rhizopodiformis, spores of streptomyces griseus, and bacterial cells of bacillus subtilis were partitioned in two-phase systems consisting of dextran, polyethylene glycol, substituted positively charged sulfonylpolyethylene glycol, and water. at a ph of 2.8 in the system, the microorganisms showed 60 to 90% affinity for the upper, polyethylene glycol-rich phase, except for cells of b. subtilis, which we ... | 1987 | 3579285 |
| [bronchocentric granulomatosis as a manifestation of allergic bronchopulmonary aspergillosis without bronchial asthma]. | a 17-year-old girl developed fever, cough and hemoptysis, as well as bouts of septic fever, dyspnea on exertion, cyanosis and weight loss. opacification in the left lower lung was a persistent feature. complement-fixation reaction to aspergillus fumigatus was 1:80, and there were four precipitation lines against this fungus on immunoelectrophoresis. aspergilli were also demonstrated in sputum and bronchial aspirate. because the patient's condition deteriorated an atypical lingula resection was p ... | 1987 | 3595464 |
| chronic granulomatous disease with cranial fungal osteomyelitis and epidural abscess. case report. | a patient is described with osteomyelitis of the cranium and epidural abscess due to aspergillus fumigatus as the presenting manifestations of chronic granulomatous disease. the diagnosis was suggested by the unusual nature of the organism isolated and confirmed by appropriate laboratory studies. the details of diagnostic assessment and therapeutic management are discussed, and the central nervous system manifestations of chronic granulomatous disease are reviewed. | 1987 | 3598663 |
| evaluation of gamma radiation levels for reducing pathogenic bacteria and fungi in animal sewage and laboratory effluents. | sewage samples collected from animal wastes and from effluents at an animal disease laboratory were inoculated with known numbers of pathogenic organisms and subjected to various doses of gamma radiation from a 60co source. surviving test organisms were quantitatively determined by selective and enrichment techniques. the experiment was modeled as a quantal assay in which probit analysis was applied to obtain d10 values. the d10 value represents the irradiating dose required to reduce the popula ... | 1987 | 3651881 |
| [bacteriological aspects of 64 cases of severe pneumonia seen in a respiratory intensive care unit]. | we report on the bacterial aspects of 64 cases of severe pneumonia in an intensive care over a two year period, excluding cases occurring during artificial ventilation. in all the cases, the grave respiratory and haemodynamic signs, the blood gas and radiological findings justified admission of these patients to an intensive care unit. specimens for bacteriology, virology and parasitology enabled a precise microbiological diagnosis 43 times (63%); in 44% of these diagnosis the possibility of the ... | 1987 | 3671863 |
| [diagnostic bronchoscopy--mycologic and bacteriologic findings]. | the authors studied the findings of mycotic and bacterial agents in the lower air passages of 122 patients with lung disease examined by bronchoscopy. the series represents a current profile of patients investigated at the department of tuberculosis and respiratory diseases, pardubice hospital within 17 months of the years 1982-1983. bronchial swabs were compared with the findings in the swabs of the throat taken immediately before the endoscopic examination. yeast organisms were isolated from t ... | 1987 | 3673125 |
| separation of mycotoxin-containing sources in grain dust and determination of their mycotoxin potential. | two distinct reservoirs of mycotoxins exist in fungal-infected cereal grains--the fungal spores and the spore-free mycelium-substrate matrix. many fungal spores are of respirable size and the mycelium-substrate matrix can be pulverized to form particles of respirable size during routine handling of grain. in order to determine the contribution of each source to the level of mycotoxin contamination of dust, we developed techniques to harvest and separate mycelium-substrate matrices from spores of ... | 1986 | 3709472 |
| fungi on the hair of large mammals in egypt. | the frequency of occurrence of fungi in 260 hair samples of dog, donkey and cow collected from veterinary medicine hospital of assiut university was determined. twenty-three species which belong to fifteen genera were collected. in low frequency, several dermatophytes (chrysosporium, microsporum, arthroderma and trichophyton) were found as well as some other fungal species pathogenic to man and animals (aspergillus fumigatus, stachybotrys chartarum, scopulariopsis brevicaulis and cephalosporium ... | 1986 | 3713793 |
| superinfection by aspergillus fumigatus of a pulmonary lesion caused by mycobacterium xenopi. | a 36-year-old man had a pulmonary infection with a cavity in the right upper lobe caused by mycobacterium xenopi. a superinfection by aspergillus fumigatus occurred 1 year later. both organisms were found in the resected lung specimen. the patient remained asymptomatic after surgery. the superinfection of healed tuberculous lesions by 'atypical' mycobacteria or aspergillus species is well known but the co-existence of the 2 organisms is very rare. | 1986 | 3715986 |
| antifungal activity of ketoconazole with emphasis on zoophilic fungal pathogens. | the antifungal activity of ketoconazole was studied against fungal pathogens associated with various mycotic infections in animals. a concentration of 10 micrograms of ketoconazole/ml of medium proved fungicidal to trichophyton verrucosum. the same concentration acted as a strong fungistat against the majority of the tested dermatophytes. yeasts were generally more sensitive than yeast-like pathogens. ketoconazole also proved fungicidal against pityrosporon canis and strongly inhibitory on crypt ... | 1986 | 3729122 |
| fowl cholera: protection against pasteurella multocida by ribosome-lipopolysaccharide vaccine. | ribosomal protein from aspergillus fumigatus substituted for intact ribosomes in potentiating the immunogenicity of pasteurella multocida lipopolysaccharide. ribosomal protein behaved as a carrier for the lipopolysaccharide. the basic protein methylated albumin, but not protamine sulfate, substituted for ribosomal protein as a carrier for lipopolysaccharide. synthetic single- and double-stranded polynucleotides did not function as an adjuvant to potentiate the immunogenicity of lipopolysaccharid ... | 1986 | 3729887 |
| detection of allergens in mould and mite preparations by a nitrocellulose electroblotting technique. | the allergen composition of moulds (alternaria alternata and aspergillus fumigatus) and mites (dermatophagoides pteronyssinus and dermatophagoides farinae) was studied by electroblotting. the allergens were separated by sds-gpage and transferred to a nitrocellulose membrane by electroblotting. specific ige antibodies directed against mould and mite allergens were utilized as probes to detect the allergens. the bands were localized on the nitrocellulose membrane by means of enzyme- or isotope-lab ... | 1986 | 3733230 |
| cerebral and orbital aspergillus infection due to invasive aspergillosis of ethmoid sinus. | aspergillus fumigatus infection due to primary infection of the ethmoid sinus affected the brain and orbit of an otherwise healthy elderly woman. primary nasal sinus infection may be a chronic process analogous to aspergilloma of the lung: it may, however, assume a locally invasive course and spread to the orbit or brain, as in the case described. this form of aspergillus infection is rare in europe but common in hot dry areas like the sudan. the infection was probably contracted by our patient ... | 1986 | 3734114 |
| [mycoflora of hay and straw]. | studies were carried out on hay and straw to establish the composition of mycoflora in them. they were sampled both at the time of harvesting and after storing for four to five months. it was found that the hay and straw were most often inseminated with spores of the fungus species alternaria tenuis, aspergillus fumigatus, mucor hiemalis, penicillium sp., and more rarely with spores of fusarium, chaetomium, scopulariopsis, cladosporium, trichoderma, and stachybotrys alternans. it was demonstrate ... | 1986 | 3739224 |
| the maternal to fetal transfer of immunoglobulins associated with placental lesions in sheep. | in this study we evaluated maternofetal transmission of immunoglobulins in ewes under conditions of altered placental morphology. intravenous injection of human red blood cells was used to induce immunoglobulins in pregnant ewes. the hemagglutination test was used to detect antibody in maternal serum, fetal and placental fluids. placental injury was induced by intravenous inoculation of escherichia coli endotoxin or spores of aspergillus fumigatus into pregnant ewes at days 99 or 100 of gestatio ... | 1986 | 3742359 |
| [aspergillosis with obstruction of the central airways]. | two cases are reported of obstruction of central airways by plugs containing aspergillus fumigatus. in one case the obstruction was located in the right stem bronchus and, despite treatment with amphotericin b, the immunocompromised patient died from invasive disseminated aspergillosis. in the other case the obstruction was in the trachea and, since the immune function of the patient was normal, she responded favorably to treatment by amphotericin b. | 1986 | 3775339 |
| circulating antibodies and antigenic cross-reactivity in hendersonula toruloidea and scytalidium hyalinum infections. | twelve (34%) of thirty-five patients with an active infection on the palms or soles caused by hendersonula toruloidea or scytalidium hyalinum were found to have circulating antibodies to these organisms by counter immunoelectrophoresis or immunodiffusion, compared with 9% of uninfected controls. in every instance there was cross-reactivity between the positive patients' sera and the heterologous non-dermatophyte antigen. using crossed and intermediate gel immunoelectrophoresis it was found that ... | 1986 | 3778813 |
| significance of igg antibodies against environmental microbial antigens in a farming population. | a group of dairy farmers studied 6 years earlier in a field survey was re-surveyed for respiratory symptoms, occupational capability and the presence of antibodies against environmental micro-organisms. specific igg antibodies to micropolyspora faeni, thermoactinomyces vulgaris, aspergillus umbrosus and aspergillus fumigatus were measured by elisa from the serum samples obtained in the two surveys. antibody titres remained constant in 70% of both farmers and controls, but where changes took plac ... | 1986 | 3779941 |
| microascus cinereus (emile-weil & gaudin) curzi--a human pathogen? | microascus cinereus is described accompanying aspergillus fumigatus in human lung tissue. the tissue contained a. fumigatus conidiophores and conidia and m. cinereus perithecia, ascospores, annellophores and conidia. the pathogenicity of m. cinereus is discussed. | 1986 | 3808006 |
| [the mycoflora of corn silage]. | maize silage was sampled to investigate the mycotic flora in it, the material for testing being taken at the time of preparing the silage as well 4 to 5 months later. it was found that richest mycoflora developed in the superficial layers of the silage mass, chiefly constituted by fungus species of the aspergillus, penicillium, alternaria, and mucor genera. it was also established that aspergillus fumigatus was the most frequently isolated species. it is believed that the development of the moul ... | 1986 | 3811222 |
| diagnostic specificity of a sandwich elisa for aspergillus-related diseases. | a sandwich elisa with specificity for a major antigen (ag 7) of aspergillus fumigatus has been compared with indirect elisas with use of crude antigenic (culture filtrate) extracts and found to have a sensitivity of 100% and specificity of 92.3% for antibody detection in sera of patients with allergic bronchopulmonary aspergillosis (abpa) and aspergilloma. in the ag 7 elisa, all sera from groups with abpa (21) and aspergilloma (15) had positive titers, and mean values for both these groups were ... | 1987 | 3819216 |
| primary cutaneous aspergillosis in six leukemic children. | we report a cluster of primary cutaneous aspergillosis in six children with hematologic malignancy. when first seen, they had hemorrhagic bullae caused by aspergillus flavus, aspergillus fumigatus, and aspergillus niger at the sites of insertion of intravenous cannulas or where arm boards had been taped to the extremities. rapid diagnosis of cutaneous aspergillosis was made by direct examination of the blister roof with potassium hydroxide before it progressed to a necrotic ulcer. intravenous am ... | 1985 | 3855880 |
| treatment of aspergillus fumigatus keratitis in rabbits with oral and topical ketoconazole. | aspergillus fumigatus keratitis was established in rabbits by intrastromal injection of a suspension containing 5,000 spores. after a 48-hour incubation period, the animals were treated with oral and topical ketoconazole alone and in combination with topical natamycin for five days. colony counts per cornea were obtained at the conclusion of the treatment period. based on these colony counts, neither oral nor topical ketoconazole was effective despite moderate in vitro sensitivity of the fungus ... | 1985 | 3872596 |
| surgery and granulocyte transfusions for life-threatening infections in chronic granulomatous disease. | we report two patients with chronic granulomatous disease (cgd) and life-threatening infections: a 10 10/12-year-old boy had aspergillus fumigatus spondylitis with destruction of the 11th vertebral body and paravertebral abscess formation, and an 8 5/12-year-old boy had multiple staphylococcus aureus hepatic abscesses with subphrenic abscess formation. both patients failed to respond to intense antimicrobial therapy but showed a remarkable recovery following surgical drainage combined with granu ... | 1985 | 3878346 |
| allergic bronchopulmonary aspergillosis. | allergic bronchopulmonary aspergillosis (abpa) occurs as a complication of asthma. it presents as an infiltrative pulmonary disorder with respiratory and systemic symptoms, eosinophilia, elevated total serum ige, and skin sensitizing, as well as precipitating antibodies to aspergillus fumigatus. sputum cultures are not always positive for the organism. if unchecked, the disease may lead to bronchiectasis and ultimately pulmonary fibrosis. therapy consists mainly of corticosteroids. | 1985 | 3880535 |
| limited reliability of the "negative" two-dimensional echocardiogram in the evaluation of infectious vegetative endocarditis: diagnostic and surgical implications. | three patients with vegetative endocarditis involving a native valve, a mechanical prosthesis and a bioprosthetic valve respectively are presented. each underwent emergency surgical exploration and prosthetic valve replacement based on clinical evidence of vegetative endocarditis after an initial delay following a report of a negative two-dimensional echocardiographic study. these cases emphasize the fact that within the clinical setting in which vegetative endocarditis is strongly suspected a n ... | 1985 | 3881454 |
| aspergilloma of the renal pelvis in a leukemic child. | | 1985 | 3881740 |
| acute rejection and massive cyclosporine requirements in heart transplant recipients treated with rifampin. | | 1985 | 3883596 |
| autoradiographic method to screen for soil microorganisms which accumulate zinc. | an autoradiographic method was developed to screen for and isolate soil microorganisms which accumulate zinc (zn). diluted soil samples (rubicon fine sand, entic haplorthods [ph 5.9]) were plated on soil extract-glucose agar containing radioactive 65zn. after 7 days of incubation, individual colonies which accumulated sufficient 65zn could be detected by autoradiography. these colonies were isolated and confirmed as zn accumulators in pure culture by using the autoradiographic plate technique. m ... | 1985 | 3883897 |
| the evaluation of a quantitative enzyme-linked immunosorbent assay (elisa) for anti-aspergillus fumigatus igg. | a standard enzyme-linked immunosorbent assay method for anti-aspergillus fumigatus igg in human serum was modified to produce a quantitative assay. the resulting assay was reproducible and capable of separating individual precipitin line groups and provided a means of monitoring the variation in antibody levels over long periods in patients with pulmonary aspergillosis. | 1985 | 3884714 |
| disseminated aspergillosis and bleeding colonic ulcers in renal transplant patient. | | 1985 | 3884810 |
| contribution to the study of aspergillus fumigatus fresenius. | 50 strains of aspergillus fumigatus were studied for enzymatic and physiologic profile. proteolytic and lipolytic activity were lacking. various ribonucleases and sacchrolytic enzymes were found. | 1985 | 3885041 |
| cardiac aspergillosis in acquired immune deficiency syndrome. | | 1985 | 3885709 |
| chemotherapy of aspergillus fumigatus keratitis: an experimental study. | an experimental keratitis study of aspergillus fumigatus was performed in 130 rabbits divided into 12 groups of ten animals each. three antifungal drugs (myconazole, amphotericin b and pimaricin) were tested using two procedures (topical drops and subconjunctival injections) and two different concentrations (500 and 10 000 times the mic). in each case, the drugs were applied every 3 h starting 14 h after inoculation. miconazole was useful at 10 mg/ml concentration by topical drops and subconjunc ... | 1985 | 3887174 |
| bovine mycotic abortion in denmark. | a survey is given of the occurrence of mycotic infections associated with abortion in danish cattle. during a period of six years a total of 748 samples of placenta material were examined. mycotic abortion was demonstrated in 101 cases (14%). the case rate was significantly higher (21%) during the winter months from december through february than during the rest of the year (10%). abortions occurred as from the 135th day of pregnancy, with maximum in the eighth month, where 45% of all cases were ... | 1985 | 3887326 |
| intracranial granuloma caused by aspergillus fumigatus. | a case is reported of intracranial granuloma caused by aspergillus fumigatus involving the anterior cranial fossa and the frontal lobe. in this case, clinical symptoms developed about 5 years before the diagnosis was made. the final diagnosis was made by a craniotomy. the patient was treated with an extensive excision and chemotherapy, but finally he failed to respond to these treatments. we compile a summary of reported cases with a tabulation of pertinent information and discuss the pathogenes ... | 1985 | 3887626 |
| galactofuranosyl groups are immunodominant in aspergillus fumigatus galactomannan. | aspergillus fumigatus galactomannan was prepared and its structure partially characterized. galactofuranosyl groups were immunodominant when this polysaccharide antigen was reacted with antibody raised in rabbits. | 1985 | 3889593 |
| surgical management of symptomatic pulmonary aspergilloma. | pulmonary aspergilloma is a potentially life-threatening disease resulting from the colonization of lung cavities by the ubiquitous fungus aspergillus fumigatus. complex aspergilloma, characterized by thick-walled cavities with surrounding parenchymal inflammation, is a risk factor for increased morbidity and mortality. fifteen patients with symptomatic aspergilloma underwent major thoracic procedures at north carolina memorial hospital between january 1, 1972, and december 31, 1983. twelve of t ... | 1985 | 3890782 |
| [diagnosis of allergic bronchopulmonary aspergillosis: report of 3 cases]. | | 1985 | 3891259 |
| serum antibodies and their role in antibody-dependent cell-mediated cytotoxicity in aspergillosis. | a total of 22 sera from patients with aspergilloma and allergic bronchopulmonary aspergillosis (abpa) were examined concomitantly for specific antibody against aspergillus fumigatus antigen and for their activity in antibody-dependent cell-mediated cytotoxicity (adcc) against aspergillus antigen-coated target cells. these sera demonstrated significant precipitin bands in agar gel double diffusion test (78% of abpa and 75% of aspergilloma sera), while in indirect immunofluorescence studies all se ... | 1985 | 3891592 |
| antibody determination against aspergillus fumigatus by means of the enzyme-linked immunosorbent assay. i. liberation of polystyrene binding components during cultivation. | the liberation of polystyrene binding antigenic components (pbc) of aspergillus fumigatus involved in elisa igg measurements was studied during cultivation in the culture filtrates. pbc were rapidly liberated into the culture medium during the logarithmic phase of growth (phase i), were in part degraded or reconsumed during the stationary phase (phase ii) and again secreted (other pbc) during the lytic phase (phase iii). elisa igg titers and numbers of precipitating components are not always rel ... | 1985 | 3891635 |
| studies on toxin of aspergillus fumigatus. xxii. fashion of binding of asp-hemolysin to human erythrocytes and asp-hemolysin-binding proteins of erythrocyte membranes. | the fashion of binding of asp-hemolysin to human erythrocytes and the isolation of asp-hemolysin-binding proteins from erythrocyte membranes were investigated by the immunocytochemical technique and affinity chromatography. asp-hemolysin bound best at a ph range from 5 to 7. the erythrocytes treated with asp-hemolysin showed diffuse, ring-like or cap-like staining by the peroxidase-labeled antibody method under the light microscope. the distribution of asp-hemolysin on the erythrocyte surface wa ... | 1985 | 3892239 |
| the many faces of pulmonary aspergillosis. | the recognition of aspergillus in all its clinical disguises remains a challenge to clinicians in all fields of medicine. fortunately, aspergillus has low pathogenicity for humans and requires very heavy inoculum of spores (aspergillary pneumonitis) in order to infect those whose pulmonary defense mechanisms, inherent structure, and physiology are intact. patients with problems from aspergillus may be seen in either inpatient or outpatient clinical practice. the patient with fibrotic or cavitary ... | 1985 | 3892565 |
| frequency of aspergillus fumigatus isolates and antibodies to aspergillus antigens in cystic fibrosis. | the frequency of aspergillus fumigatus isolates from sputum was assessed prospectively during a 22-month period in 156 patients with cystic fibrosis (cf) from one center, and findings were compared to a cross-sectional evaluation of specific igg and iga antibodies, occurrence of chronic pseudomonas aeruginosa infection, and pulmonary function. the prevalence rate for the 22-month period was 40%. positive a. fumigatus cultures appeared to be independent of the presence or duration of chronic bron ... | 1985 | 3893030 |
| embolotherapy of a mycotic pseudoaneurysm of the internal mammary artery in chronic granulomatous disease. | we report a case demonstrating an unusual and previously unpublished complication of chronic granulomatous disease (cgd), that is massive hemorrhage from a mycotic pseudoaneurysm of the left internal mammary due to invasive aspergillus fumigatus of the anterior chest wall. this brief report further illustrates the angiographic diagnosis and embolotherapeutic control of a mycotic pseudoaneurysm of the internal mammary artery. | 1985 | 3893696 |
| rapid immunoelectrophoretic assay for detection of serum antibodies to aspergillus fumigatus catalase in patients with pulmonary aspergillosis. | a rapid immunoelectrophoretic assay was developed to detect antibodies to aspergillus fumigatus catalase. the method's diagnostic sensitivity for pulmonary aspergillosis was 88% (72-97%, 95% confidence limits) in 33 patients presenting with either aspergilloma or aspergillus lung infiltrate. the diagnostic specificity was 94% (90-97%) as judged from 191 patients with other infiltrative lung diseases, including infections and neoplasia. none of the 185 healthy subjects had catalase antibodies. th ... | 1985 | 3894017 |
| angioinvasive pulmonary aspergillosis: presentation as massive pulmonary saddle embolism in an immunocompromised patient. | a 33 year old woman with chronic myelogenous leukemia presented with clinical symptoms and hemodynamic signs suggestive of pulmonary embolism. initial angiographic studies supported the diagnosis of a massive saddle pulmonary embolus, and an inferior vena cava filter was inserted. however, subsequent autopsy revealed unsuspected angioinvasive pulmonary aspergillosis with secondary in situ thrombosis. the clinical features and diagnostic considerations in immunocompromised patients presenting wit ... | 1985 | 3894476 |
| [a case of aspergillosis of the ear]. | | 1985 | 3895133 |
| mycotic pseudoaneurysm of an aortic bypass graft and contiguous vertebral osteomyelitis due to aspergillus fumigatus. | fungal osteomyelitis is rare in patients who are not immunocompromised. this report documents that aspergillus vertebral osteomyelitis may, however, be associated with infection of an adjacent prosthetic vascular graft in the absence of overt immunosuppression. a 73-year-old man is described with a mycotic pseudoaneurysm of a dacron aortic bypass graft and contiguous vertebral osteomyelitis due to aspergillus fumigatus. the patient was successfully treated with resection of the infected graft an ... | 1985 | 3895911 |
| treatment of aspergillus fumigatus keratitis in rabbits with oral and topical ketoconazole. | | 1985 | 3895970 |
| dual mycotic infection in a chicken. | a double mycotic infection, caused by aspergillus fumigatus and a zygomycete-like organism, was histopathologically demonstrated in the lungs of a 12-week-old chicken. a. fumigatus was isolated, but the zygomycete was not. | 1985 | 3896222 |
| [fungal pneumonia]. | | 1985 | 3897098 |
| [nasosinus aspergillosis]. | | 1985 | 3898267 |
| the occurrence of and organisms concerned with bovine mycotic abortion in some counties of ireland. | bovine mycotic abortion in some counties of the irish provinces of leinster and ulster was estimated at 1-2% of all abortions for which a causal agent was identified in the years from 1969 to 1978. the organism most commonly isolated from diseased specimens was aspergillus fumigatus, followed by absidia corymbifera. there was no relationship between the incidence of the disease in any year and the rainfall or number of raindays during the previous summer. the data obtained are discussed in relat ... | 1985 | 3898556 |
| aspergillus fumigatus thrombi causing total occlusion of both coronary arterial ostia, all four major epicardial coronary arteries and coronary sinus and associated with purulent pericarditis. | | 1985 | 3898801 |
| detection of class-specific antibodies against aspergillus fumigatus antigens in various pulmonary diseases. | using an enzyme-linked immunosorbent assay (elisa) and a radioimmunoassay (rast), the specific iga, igg, igm and ige antibody levels against aspergillus fumigatus antigens were determined in sera from patients with either chronic bronchitis or non-allergic bronchial asthma, allergic asthma, allergic bronchopulmonary aspergillosis or aspergilloma. these levels were compared with those in serum samples from healthy controls. the results showed statistical differences in specific antibody levels be ... | 1985 | 3898993 |
| [enzyme-linked immunosorbent assay of serum igg antibodies to aspergillus fumigatus antigen using mouse monoclonal antibody to human igg]. | | 1985 | 3899058 |
| igg enzyme linked immunosorbent assay for diagnosis of invasive aspergillosis: retrospective study over 15 years of transplant recipients. | four commercially available aspergillus fumigatus antigens were compared in an igg enzyme linked immunosorbent assay by testing multiple sera from 19 patients who had received renal and hepatic allografts and who had histologically confirmed invasive aspergillosis. raised antibody levels were found in 16 of the 19 patients. more antibody responses were detected by culture filtrate antigens, but somatic antigens often detected antibodies sooner. when antibodies to aspergillus antigens were presen ... | 1985 | 3900143 |
| the diagnosis of invasive aspergillosis by an enzyme-linked immunosorbent assay for circulating antigen. | an inhibition enzyme-linked immunosorbent assay (elisa) capable of detecting 10 ng of aspergillus carbohydrate antigen/ml of serum was developed. when retrospectively applied to the sera of 19 patients with invasive aspergillosis, the elisa detected antigen in 11 patients. none of 14 healthy controls or 28 patients with a variety of other infections were positive for circulating antigen. a rabbit model of invasive aspergillosis was also developed. daily fungal cultures of blood were negative in ... | 1985 | 3900240 |
| [detection of aspergillosis among patients with lung diseases]. | | 1985 | 3900990 |