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the first german map of georeferenced ixodid tick locations.georeferenced locations of ixodid ticks are required to depict the observed distribution of species. further, they are used as input data for species distribution models also known as niche models. the latter were applied to describe current and future (projected) tick distributions. beside model assumptions and selected climate parameters, the number of georeferenced tick locations available as a digital dataset is of fundamental importance for the reliability of such models. for germany, howev ...201425301245
ectoparasite infestations and canine infection by rickettsiae and ehrlichiae in a semi-arid region of northeastern brazil.this study investigated the prevalence of rickettsia spp. and ehrlichia canis infection in dogs and their ectoparasites from rural and urban areas of two municipalities, petrolina and juazeiro, within a semiarid region (caatinga biome) of northeastern brazil, by immunofluorescence assay (ifa) and polymerase chain reaction (pcr). overall, 12.1% (61/504) and 23.0% (116/504) of canine plasma samples had antibodies reactive to rickettsia spp. and e. canis. e. canis dna was detected by pcr in 8.3% (4 ...026565771
tick-borne rickettsial pathogens in questing ticks, removed from humans and animals in mexico.tick-borne rickettsial diseases (tbrd) are commonly encountered in medical and veterinary clinical settings. the control of these diseases is difficult, requiring disruption of a complex transmission chain involving a vertebrate host and ticks. the geographical distribution of the diseases is related to distribution of the vector, which is an indicator of risk for the population. a total of 1107 were collected by tick drag from forests, ecotourism parks and hosts at 101 sites in 22 of the 32 sta ...026726019
candidatus rickettsia andeanae, a spotted fever group agent infecting amblyomma parvum ticks in two brazilian biomes.adult ticks of the species amblyomma parvum were collected from the vegetation in the pantanal biome (state of mato grosso do sul) and from horses in the cerrado biome (state of piauí) in brazil. the ticks were individually tested for rickettsial infection via polymerase chain reaction (pcr) targeting three rickettsial genes, glta, ompa and ompb. overall, 63.5% (40/63) and 66.7% (2/3) of a. parvum ticks from pantanal and cerrado, respectively, contained rickettsial dna, which were all confirmed ...024714968
rickettsia rickettsii isolation from naturally infected amblyomma parvum ticks by centrifugation in a 24-well culture plate technique.rocky mountain spotted fever is an acute illness caused by rickettsia rickettsii (r. rickettsii) and is transmitted by the bite of ticks of the genera dermacentor, amblyomma and rhipicephalus. the illness results in a high mortality rate and may be easily confused with other febrile syndromes. in yucatan state, mexico, childhood cases with a high mortality have been reported. in this work we report the isolation of a mexican r. rickettsii strain from a tick egg mass using an alternative method f ...201326623321
spotted fever group rickettsia in the pampa biome, brazil, 2015-2016. 027767913
rickettsia parkeri rickettsiosis, arizona, usa.in the united states, all previously reported cases of rickettsia parkeri rickettsiosis have been linked to transmission by the gulf coast tick (amblyomma maculatum). here we describe 1 confirmed and 1 probable case of r. parkeri rickettsiosis acquired in a mountainous region of southern arizona, well beyond the recognized geographic range of a. maculatum ticks. the likely vector for these 2 infections was identified as the amblyomma triste tick, a neotropical species only recently recognized in ...027089251
histoarchitecture of the ovary of rhipicephalus (boophilus) annulatus during pre- and postengorgement period.the present communication describes the detailed day wise study of histological changes of the ovary of rhipicephalus (boophilus) annulatus in the postengorgement period together with the systematic classification of their oocytes. the ovary of r. (b.) annulatus is panoistic type with an asynchronous development of oocytes. all the stages (ii, iii, iv, and v) of oocytes except stage i were similar to r. (b.) microplus. the stage i oocytes showed basophilia, which was not reported earlier in othe ...201525664337
morphological and molecular identification of the brown dog tick rhipicephalus sanguineus and the camel tick hyalomma dromedarii (acari: ixodidae) vectors of rickettsioses in egypt.rickettsioses have an epidemiological importance that includes pathogens, vectors, and hosts. the dog tick rhipicephalus sanguineus and the camel tick hyalomma dromedarii play important roles as vectors and reservoirs of rickettsiae. the aim of this study was to determine the prevalence of rickettsiae in ixodid ticks species infesting dogs and camels in egypt, in addition to, the morphological and molecular identification of r. sanguineus and h. dromedarii.201627847418
native strains of beauveria bassiana for the control of rhipicephalus sanguineus sensu lato.rhipicephalus sanguineus sensu lato ticks are widespread worldwide due to their adaptability to survive under different environmental conditions. they may act as vectors of a wide range of pathogens to humans and animals and their control is based on the use of chemical products on dogs and in the environment. alternative control strategies, such as the use of entomopathogenic fungi as bio-control agents have also been investigated. the ability of native strains of beauveria bassiana sensu lato ...201525651851
kampinos national park: a risk area for spotted fever group rickettsioses, central poland?ixodid ticks are important vectors of a variety of bacterial and protozoan pathogens which cause infections in humans. in this study, altogether 1041 questing ixodes ricinus (n = 305) and dermacentor reticulatus ticks (n = 736), sympatrically occurring in kampinos national park (kpn), central-east poland, were analyzed by pcr for rickettsia species. overall, the pathogen prevalence in ticks was 27.5 % for i. ricinus and 42.8 % for d. reticulatus. sequencing analysis showed that the first tick sp ...201627631765
anaplasma infection of bactrian camels (camelus bactrianus) and ticks in xinjiang, china.to date, anaplasmosis has been reported to be a subclinical disease in indian and arabian one-humped camels (camelus dromedarius) and llamas (lama glama). however, no information on anaplasma infection in two-humped bactrian camels (camelus bactrianus) in china has been published to date. the aim of this study was to investigate the prevalence of anaplasma spp. in domestic bactrian camels and ticks in xinjiang, china.201526055661
sensitivity to permethrin in a dermacentor reticulatus population from eastern poland in laboratory study.the action of chemical compounds on the palaearctic tick d. reticulatus (fabricius) (acari: amblyomminae) has been poorly investigated so far. therefore, the effects of application of permethrin on engorged d. reticulatus females have been assessed, and the survival rate for the different developmental stages of the tick species in its non-parasitic phase of the life cycle was determined upon application of the pyrethroid.201424405550
clustered cases of rickettsia sibirica mongolitimonae infection, france. 023460995
ixodes pacificus ticks maintain embryogenesis and egg hatching after antibiotic treatment of rickettsia endosymbiont.rickettsia is a genus of intracellular bacteria that causes a variety of diseases in humans and other mammals and associates with a diverse group of arthropods. although rickettsia appears to be common in ticks, most rickettsia-tick relationships remain generally uncharacterized. the most intimate of these associations is rickettsia species phylotype g021, a maternally and transstadially transmitted endosymbiont that resides in 100% of i. pacificus in california. we investigated the effects of t ...201425105893
experimental infection of rhipicephalus sanguineus with ehrlichia chaffeensis.ehrlichia chaffeensis, the etiologic agent of human monocytic ehrlichiosis, is a tick-borne rickettsial pathogen that is infective to a wide range of mammals, including dogs and people. amblyomma americanum, the lone star tick, is considered the primary vector of e. chaffeensis, but this pathogen has been detected in other tick species, including the brown dog tick, rhipicephalus sanguineus. we hypothesized that the arkansas strain of e. chaffeensis is infective to r. sanguineus, and used a nove ...201424894131
climate and environmental change drives ixodes ricinus geographical expansion at the northern range margin.global environmental change is causing spatial and temporal shifts in the distribution of species and the associated diseases of humans, domesticated animals and wildlife. in the on-going debate on the influence of climate change on vectors and vector-borne diseases, there is a lack of a comprehensive interdisciplinary multi-factorial approach utilizing high quality spatial and temporal data.201424401487
an evidence-based review on medicinal plants used as insecticide and insect repellent in traditional iranian medicine.insects can be the cause of major ecological problems; they can transmit microbes and parasites that affect humans, and damage food crops, trees, and homes. the total economic cost of insect-related damage and disease is immeasurable. in traditional iranian medicine (tim), several medicinal plants have been identified as insecticides or insect repellents, but many of them are still unknown. therefore, the aim of this study was to review the insecticidal or insect repellent activity of certain me ...201627186389
saliva from nymph and adult females of haemaphysalis longicornis: a proteomic study.haemaphysalis longicornis is a major vector of theileria spp., anaplasma phagocytophilum, babesia spp. and coxiella burnetti in east asian countries. all life stages of ixodid ticks have a destructive pool-feeding style in which they create a pool-feeding site by lacerating host tissue and secreting a variety of biologically active compounds that allows the tick to evade host responses, enabling the uptake of a blood meal. the identification and functional characterization of tick saliva protein ...201526104117
systematic revision of the adeleid haemogregarines, with creation of bartazoon n. g., reassignment of hepatozoon argantis garnham, 1954 to hemolivia, and molecular data on hemolivia stellata.life cycles and molecular data for terrestrial haemogregarines are reviewed in this article. collection material was re-examined: hepatozoon argantis garnham, 1954 in argas brumpti was reassigned to hemolivia as hemolivia argantis (garnham, 1954) n. comb.; parasite dna was extracted from a tick crush on smear of an archived slide of hemolivia stellata in amblyomma rotondatum, then the 18s ssrrna gene was amplified by pcr. a systematic revision of the group is proposed, based on biological life c ...201526551414
hard tick species of livestock and their bioecology in golestan province, north of iran.a survey on tick species composition was carried out in golestan province iran during year 2010-2011.the aim was to determine tick species parasitizing domestic ruminants and their seasonal population dynamics.201325629071
crimean-congo hemorrhagic fever in iran.the presence of crimean-congo hemorrhagic fever virus (cchfv) in iran was first identified in studies of livestock sera and ticks in the 1970s, but the first human infection was not diagnosed until 1999. since that time, the number of cases of cchf in iran has markedly increased. through january 2012, articles in the published literature have reported a total of 870 confirmed cases, with 126 deaths, for a case fatality rate (cfr) of 17.6%. the disease has been seen in 26 of the country's 31 prov ...201323872313
hard ticks on domestic ruminants and their seasonal population dynamics in yazd province, iran.ticks are the main vectors for transmission of different pathogens to human and animals. this survey was performed to find out distribution of ticks, which infested the domestic ruminants in yazd province, central iran during year 2008-2009.201022808391
cockroach, tick, storage mite and other arthropod allergies: where do we stand with molecular allergy diagnostics?: part 15 of the series molecular allergology.arthropods form a broad phylum within the animal kingdom, comprising widely varying members such as insects, arachnids, crabs and centipedes. in addition to common allergies to house dust mites or hymenoptera venom, there are also rarer allergies that can be attributed to three major sources of allergens: cockroaches, ticks and storage mites. other less known allergen sources include spiders, mosquitos, horseflies, red chironomid larvae, silverfish and ladybugs, as well as a variety of storage p ...201426146603
detection of rickettsia parkeri from within piura, peru, and the first reported presence of candidatus rickettsia andeanae in the tick rhipicephalus sanguineus.domestic farm animals (n=145) were sampled for the presence of ectoparasites in northwestern peru during march, 2008. ninety domestic animals (62%) were positive for the presence of an ectoparasite(s) and produced a total collection of the following: 728 ticks [amblyomma maculatum, anocentor nitens, rhipicephalus (boophilus) microplus, rhipicephalus sanguineus, and otobius megnini], 12 lice (haematopinus suis), and 3 fleas (ctenocephalides felis). a rickettsia genus-specific qpcr assay was perfo ...201323488453
effect of the saliva from different triatomine species on the biology and immunity of tlr-4 ligand and trypanosoma cruzi-stimulated dendritic cells.triatomines are blood-sucking vectors of trypanosoma cruzi, the causative agent of chagas disease. during feeding, triatomines surpass the skin host response through biomolecules present in their saliva. dendritic cells (dcs) play a crucial role in the induction of the protection to aggressive agents, including blood-sucking arthropods. here, we evaluated if salivary components of triatomines from different genera evade the host immunity by modulating the biology and the function of lps- or t. c ...201627938380
an update on the epidemiological situation of spotted fever in brazil.spotted fever is a tick-borne rickettsial disease. in brazil, its notification to the ministry of health is compulsory. since 2007, cases of spotted fever have been integrated to the notifiable diseases information system, and epidemiological analyzes are part of the routines on surveillance programs.201627555867
frequency and distribution of rickettsiae, borreliae, and ehrlichiae detected in human-parasitizing ticks, texas, usa.to describe the presence and distribution of tickborne bacteria and their vectors in texas, usa, we screened ticks collected from humans during 2008-2014 for rickettsia, borrelia, and ehrlichia spp. thirteen tick species were identified, and 23% of ticks carried bacterial dna from at least 1 of the 3 genera tested.026811941
immunosuppressive effects of amblyomma cajennense tick saliva on murine bone marrow-derived dendritic cells.dendritic cells (dcs) are professional antigen-presenting cells with vital roles in the activation of host immunity. ticks are bloodsucking arthropods that secrete bioactive compounds with immunomodulatory properties via their saliva. it is known that some tick species modulate the biology of dcs with different intensities; however, studies on amblyomma cajennense, the cayenne tick, have not yet been performed, although this species is considered one of the most capable of modulating immune resp ...201525586117
tick-borne lymphadenopathy-like condition in an african woman in kenya.a 30-year-old african woman in kenya succumbed to severe swollen regional lymph nodes, development of painful boils and ulcer formation and rashes at specific tick-biting sites together with an intermittent fever and headache following repeated tick bites of rhipicephalus pulchellus. she later developed nuchal lymphadenopathy-like condition and an eschar with edematous margins at bitten sites. a sustained high fever and fatigue then followed. she became well after treatment with antibiotics and ...024497868
phylogeography and demographic history of amblyomma variegatum (fabricius) (acari: ixodidae), the tropical bont tick.the genetic diversity of amblyomma variegatum (fabricius) from four caribbean islands and five african countries was compared by analyzing the sequences of three gene fragments, two mitochondrial (12srdna and d-loop-dl), and one nuclear (intergenic transcribed spacer 2 [its2]). genetic variability of the its2 dna fragment consisted of only uninformative single nucleotide mutations, and therefore this gene was excluded from further analyses. mitochondrial gene divergences among african population ...201222448720
the environmental-endocrine basis of gynandromorphism (intersex) in a crustacean.commensurate with the decline in many crustacean populations has been an accumulation in reports of sexually ambiguous individuals within these populations. the cause of gynandromorphism or intersex among crustaceans is unknown. we show that gynandromorphism in the branchiopod crustacean daphnia magna is initiated by the sex-determining hormone methyl farnesoate when levels of the hormone are intermediate between low levels that stimulate the production of broods containing all female offspring ...200617205107
ectoparasites of stray dogs in mazandaran, gilan and qazvin provinces, north and center of iran.the aim of the present study was to determine the prevalence of ectoparasite infestations in stray dogs in mazandaran, gilan and qazvin provinces in fall and winter in 2013(december to march).201627308294
high seroprevalence for spotted fever group rickettsiae, is associated with higher temperatures and rural environment in mbeya region, southwestern tanzania.rickettsioses are endemic in sub-sahara africa. burden of disease, risk factors and transmission are hitherto sparsely described.201525849718
molecular detection of tick-borne pathogens in captive wild felids, zimbabwe.the populations of wild felids in africa, of especially lions (panthera leo) and cheetahs (acinonyx jubatus), are declining and the species are classified as vulnerable to extinction by the international union for conservation of nature. as infections with tick-borne pathogens (tbp) can become more of a problem in wild felids, there are relatively few studies on tbp in wild felids in africa and on how these infections might influence population numbers.201425404084
are ticks venomous animals?as an ecological adaptation venoms have evolved independently in several species of metazoa. as haematophagous arthropods ticks are mainly considered as ectoparasites due to directly feeding on the skin of animal hosts. ticks are of major importance since they serve as vectors for several diseases affecting humans and livestock animals. ticks are rarely considered as venomous animals despite that tick saliva contains several protein families present in venomous taxa and that many ixodida genera ...201425006341
tick infestation patterns in free ranging african buffalo (syncercus caffer): effects of host innate immunity and niche segregation among tick species.ticks are of vast importance to livestock health, and contribute to conflicts between wildlife conservation and agricultural interests; but factors driving tick infestation patterns on wild hosts are not well understood. we studied tick infestation patterns on free-ranging african buffalo (syncercus caffer), asking (i) is there evidence for niche segregation among tick species?; and (ii) how do host characteristics affect variation in tick abundance among hosts? we identified ticks and estimated ...201224533310
rangelia vitalii in a free-ranging maned wolf (chrysocyon brachyurus) and co-infections.an adult free-ranged female maned wolf was rescued from a periurban area subject to anthropogenic disturbances in the minas gerais, brazil. the animal presented poor body condition and anemia. the clinical condition rapidly deteriorated culminating in dead and a necropsy was performed. the main gross lesions were marked anemia and blood content in the intestines accompanied by many types of parasites. the protozoa rangelia vitalii was identified by histopathological analysis predominantly within ...201627761403
effect of amblyomma maculatum (acari: ixodidae) saliva on the acute cutaneous immune response to rickettsia parkeri infection in a murine model.rickettsia parkeri luckman (rickettsiales: rickettsiaceae) is a pathogenic spotted fever group rickettsia transmitted by amblyomma maculatum koch (acari: ixodidae) in the united states. the acute innate immune response to this pathogen and the effect of tick feeding or salivary components on this response is largely unknown. we hypothesized that a. maculatum saliva enhances r. parkeri infection via downregulation of the acute cellular and cytokine immune response. c3h/hen mice were intradermally ...201627521760
comparative speed of kill of sarolaner (simparica™ chewables) and fluralaner (bravecto(®)) against induced infestations of amblyomma americanum on dogs.the lone star tick, amblyomma americanum, infests dogs and cats in north america and transmits the pathogens ehrlichia chaffeensis and ehrlichia ewingii, which cause monocytic and granulocytic ehrlichiosis in dogs and humans, and cytauxzoon felis which causes cytauxzoonosis in cats. a parasiticide's speed of kill is important to minimize the direct deleterious effects [related to blood-feeding] of tick infestation and reduce the risk of transmission of tick-borne pathogens. in this study the spe ...201627430425
evasins: therapeutic potential of a new family of chemokine-binding proteins from ticks.blood-sucking parasites, such as ticks, remain attached to their hosts for relatively long periods of time in order to obtain their blood meal without eliciting an immune response. one mechanism used to avoid rejection is the inhibition of the recruitment of immune cells, which can be achieved by a class of chemokine-binding proteins (ckbps) known as evasins. we have identified three distinct evasins produced by the salivary glands of the common brown dog tick, rhipicephalus sanguineus. they dis ...201627375615
comparative speed of kill of oral treatments with simparica™ (sarolaner) and bravecto®(fluralaner) against induced infestations of rhipicephalus sanguineus on dogs.rhipicephalus sanguineus is the most widely distributed tick species infesting dogs worldwide, which may cause discomfort to the host and transmit diseases. acaricides with a rapid and sustained speed of kill are thus important to prevent infestation and to reduce the risk of disease transmission. in this study, the speed of kill of a monthly administered simparica™ (sarolaner) treatment against induced infestations with r. sanguineus on dogs was evaluated and compared with a single dose of brav ...201626911244
expanding range of amblyomma americanum and simultaneous changes in the epidemiology of spotted fever group rickettsiosis in the united states.spotted fever group (sfg) rickettsia species are etiologic agents of a wide range of human infections from asymptomatic or mild infections to severe, life-threatening disease. in the united states, recent passive surveillance for sfg rickettsiosis shows an increased incidence and decreased severity of reported cases. the reasons for this are not well understood; however, we hypothesize that less pathogenic rickettsiae are causing more human infections, while the incidence of disease caused by mo ...201526503270
spotted fever group rickettsiae in multiple hard tick species from fairfax county, virginia.spotted fever group rickettsiosis (sfgr) is a potentially fatal disease that has displayed increasing incidence in the united states in recent years. the most well-known and severe type of this disease is rocky mountain spotted fever, but there are other mild forms that occur. recently, human infection with rickettsia parkeri has been reported and linked with the tick amblyomma maculatum. in 2010, a population of r. parkeri-infected a. maculatum was discovered in fairfax county, virginia, leadin ...201424978651
functional characterisation of a nicotinic acetylcholine receptor α subunit from the brown dog tick, rhipicephalus sanguineus.ticks and tick-borne diseases have a major impact on human and animal health worldwide. current control strategies rely heavily on the use of chemical acaricides, most of which target the cns and with increasing resistance, new drugs are urgently needed. nicotinic acetylcholine receptors (nachrs) are targets of highly successful insecticides. we isolated a full-length nachr α subunit from a normalised cdna library from the synganglion (brain) of the brown dog tick, rhipicephalus sanguineus. phyl ...201324291321
redescription of hepatozoon felis (apicomplexa: hepatozoidae) based on phylogenetic analysis, tissue and blood form morphology, and possible transplacental transmission.a hepatozoon parasite was initially reported from a cat in india in 1908 and named leucocytozoon felis domestici. although domestic feline hepatozoonosis has since been recorded from europe, africa, asia and america, its description, classification and pathogenesis have remained vague and the distinction between different species of hepatozoon infecting domestic and wild carnivores has been unclear. the aim of this study was to carry out a survey on domestic feline hepatozoonosis and characteriz ...201323587213
efficacy of an imidacloprid/flumethrin collar against fleas, ticks, mites and lice on dogs.the studies reported here were conducted to ascertain the efficacy of imidacloprid/flumethrin incorporated in a slow-release matrix collar, against infestations of dogs by fleas, ticks, mites and lice. efficacy was evaluated against the flea ctenocephalides felis felis, the ticks rhipicephalus sanguineus, ixodes ricinus, ixodes scapularis, dermacentor reticulatus and dermacentor variabilis, the mite sarcoptes scabiei and the biting louse trichodectes canis.201222647530
induction of humoral immune response to multiple recombinant rhipicephalus appendiculatus antigens and their effect on tick feeding success and pathogen transmission.rhipicephalus appendiculatus is the primary vector of theileria parva, the etiological agent of east coast fever (ecf), a devastating disease of cattle in sub-saharan africa. we hypothesized that a vaccine targeting tick proteins that are involved in attachment and feeding might affect feeding success and possibly reduce tick-borne transmission of t. parva. here we report the evaluation of a multivalent vaccine cocktail of tick antigens for their ability to reduce r. appendiculatus feeding succe ...201627589998
arsenophonus nasoniae and rickettsiae infection of ixodes ricinus due to parasitic wasp ixodiphagus hookeri.arsenophonus nasoniae, a male-killing endosymbiont of chalcid wasps, was recently detected in several hard tick species. following the hypothesis that its presence in ticks may not be linked to the direct occurrence of bacteria in tick's organs, we identified a. nasoniae in wasps emerging from parasitised nymphs. we confirmed that 28.1% of ixodiphagus hookeri wasps parasitizing ixodes ricinus ticks were infected by a. nasoniae. moreover, in examined i. ricinus nymphs, a. nasoniae was detected on ...201626901622
in vitro effect of the ethanolic extract of tephrosia vogelii on rhipicephalus sanguineus in abomey-calavi.ticks are vectors of several diseases, of which many are zoonosis transmissible to humans. the use of tephrosia leafs' extract as a low cost acaricide is spreading among farmers in central kenya.026101758
high prevalence of rickettsia africae variants in amblyomma variegatum ticks from domestic mammals in rural western kenya: implications for human health.tick-borne spotted fever group (sfg) rickettsioses are emerging human diseases caused by obligate intracellular gram-negative bacteria of the genus rickettsia. despite being important causes of systemic febrile illnesses in travelers returning from sub-saharan africa, little is known about the reservoir hosts of these pathogens. we conducted surveys for rickettsiae in domestic animals and ticks in a rural setting in western kenya. of the 100 serum specimens tested from each species of domestic r ...025325312
occurrence of tick-transmitted pathogens in dogs in jos, plateau state, nigeria.canine babesiosis caused by babesia rossi, transmitted by haemaphysalis elliptica in south africa, has also been reported from nigeria. although h. leachi (sensu lato) is widespread in sub-saharan africa, published literature on the occurrence of canine babesiosis is meagre. it has been postulated that the genotype of babesia rossi erythrocyte membrane antigen 1 (brema1) may be linked to virulence of the specific isolate. the primary objective of this study was to detect and characterise tick-bo ...201424661795
toxicity of millettia ferruginea darasana (family: fabaceae) against the larvae and adult ticks of amblyomma variegatum fabricius a three-host tick in cattle.the in vitro toxicity of millettia ferruginea darasana (family: fabaceae) was tested against the larvae adult male and female of a three-host tick, amblyomma variegatum fabricius (family: ixodidae or hard tick), known as 'tropical bont tick' parasitic mainly to cattle found in ethiopia and other equatorial africa. the 20, 40, 60, 80 and 100 % concentrations of the seed oil extracted with petroleum ether were found to kill all (100 % mortality) larvae after 12, 9, 6, 3 and 1.5 h respectively. the ...201326064022
ixodid ticks of traditionally managed cattle in central nigeria: where rhipicephalus (boophilus) microplus does not dare (yet?).ticks and tick-borne diseases (tbds) undermine cattle fitness and productivity in the whole of sub-saharan africa, including nigeria. the aim of this study was to document the composition of tick species, assessing the burden of infestation, in traditionally managed cattle in an area of central nigeria where acaricides have not been used historically.201323758913
simple method to differentiate among rickettsia species.in this work we present a new option to identify 11 rickettsial species that cause human rickettsioses, with some advantages over the previous methods described. using rickettsial isolates from 11 rickettsia species as a sample, we used the polymerase chain reaction to amplify a 990- to 1,000-bp dna fragment from the ompb gene, common for the 11 rickettsia species analyzed in this study, which were digested with alui restriction enzyme to obtain different digestion patterns. this restriction pat ...201323595041
coxiella burnetii in humans, domestic ruminants, and ticks in rural western kenya.we conducted serological surveys for coxiella burnetii in archived sera from patients that visited a rural clinic in western kenya from 2007 to 2008 and in cattle, sheep, and goats from the same area in 2009. we also conducted serological and polymerase chain reaction-based surveillance for the pathogen in 2009-2010, in human patients with acute lower respiratory illness, in ruminants following parturition, and in ticks collected from ruminants and domestic dogs. antibodies against c. burnetii w ...201323382156
comparison of real-time quantitative pcr and culture for the diagnosis of emerging rickettsioses.isolation of rickettsia species from skin biopsies may be replaced by pcr. we evaluated culture sensitivity compared to pcr based on sampling delay and previous antibiotic treatment.201222413026
rhipicephalus (boophilus) microplus aquaporin as an effective vaccine antigen to protect against cattle tick infestations.vaccination as a control method against the cattle tick, rhipicephalus (boophilus) microplus has been practiced since the introduction of two products in the mid-1990s. there is a need for a vaccine that could provide effective control of r. microplus in a more consistent fashion than existing products. during our transcriptome studies of r. microplus, several gene coding regions were discovered to encode proteins with significant amino acid similarity to aquaporins.201425306139
anaplasma infection in ticks, livestock and human in ghaemshahr, mazandaran province, iran.anaplasmosis is an important issue for animal breeders in terms of economic losses as well as a health concern to human. ticks are considered as the main vector of this disease. lack of documented information about anaplasma species in iran was the scope of this study to determine the population of ticks and the presence of anaplasma in ticks, domestic ruminants and also human beings in northern iran.201426114134
ticks infesting domestic dogs in the uk: a large-scale surveillance programme.recent changes in the distribution of tick vectors and the incidence of tick-borne disease, driven variously by factors such as climate change, habitat modification, increasing host abundance and the increased movement of people and animals, highlight the importance of ongoing, active surveillance. this paper documents the results of a large-scale survey of tick abundance on dogs presented to veterinary practices in the uk, using a participatory approach that allows relatively cost- and time-eff ...201627388169
a review of canine babesiosis: the european perspective.canine babesiosis is a significant tick-borne disease caused by various species of the protozoan genus babesia. although it occurs worldwide, data relating to european infections have now been collected for many years. these data have boosted the publication record and increased our working knowledge of these protozoan parasites. both the large and small forms of babesia species (b. canis, b. vogeli, b. gibsoni, and b. microti-like isolates also referred to as "b. vulpes" and "theileria annae") ...201627289223
update on epidemiology of canine babesiosis in southern france.canine babesiosis is an emerging or re-emerging disease caused by babesia and theileria protozoans, also called piroplasms, transmitted by ixodid ticks. in europe, four etiological agents have been identified to date, namely babesia canis, b. vogeli, b. gibsoni and theileria annae. france has a high prevalence of canine babesiosis and two tick species, dermacentor reticulatus and rhipicephalus sanguineus, are supposed to transmit b. canis and b. vogeli respectively. in southern france, where dog ...201526303260
reclassification of theileria annae as babesia vulpes sp. nov.theileria annae is a tick-transmitted small piroplasmid that infects dogs and foxes in north america and europe. due to disagreement on its placement in the theileria or babesia genera, several synonyms have been used for this parasite, including babesia spanish dog isolate, babesia microti-like, babesia (theileria) annae, and babesia cf. microti. infections by this parasite cause anemia, thrombocytopenia, and azotemia in dogs but are mostly subclinical in red foxes (vulpes vulpes). furthermore, ...201525890372
a molecular survey of vector-borne pathogens in red foxes (vulpes vulpes) from bosnia and herzegovina.red foxes (vulpes vulpes) have recently been recognized as potential reservoirs of several vector-borne pathogens and a source of infection for domestic dogs and humans, mostly due to their close vicinity to urban areas and frequent exposure to different arthropod vectors. the aim of this study was to investigate the presence and distribution of babesia spp., hepatozoon canis, anaplasma spp., bartonella spp., 'candidatus neoehrlichia mikurensis', ehrlichia canis, rickettsia spp. and blood filaro ...201525889961
fox on the run--molecular surveillance of fox blood and tissue for the occurrence of tick-borne pathogens in austria.the red fox (vulpes vulpes) is a widespread species, harbouring many pathogens relevant for humans and pets. indeed, anaplasma spp., ehrlichia canis and rickettsia spp. among the bacteria and hepatozoon canis as well as babesia sp. among the parasites have been the focus of several studies.201425413694
questionnaire-based survey on the distribution and incidence of canine babesiosis in countries of western europe.the incidence of canine babesiosis may vary considerably from one country to another depending on the distribution of the causative parasite species and their specific vectors. the aim of the present study was to evaluate the clinical occurrence of canine babesiosis diagnosed in european veterinary clinics and propose an updated map of the disease distribution in western europe. questionnaires were sent to companion animal veterinary clinics in spain, france, benelux, germany and austria. the an ...201424626325
a randomized, blinded, controlled and multi-centered field study comparing the efficacy and safety of bravecto (fluralaner) against frontline (fipronil) in flea- and tick-infested dogs.fluralaner, a new molecular entity of the isoxazoline class, has potent insecticidal and acaricidal activity and can be safely administered orally to dogs.201424593931
theileria annae in a young swedish dog.a severe regenerative anemia was detected in a 12-week-old mixed breed puppy in sweden. a small protozoan parasite was observed in erythrocytes on a blood smear. it was initially suspected to be babesia gibsoni based on its size and because b. gibsoni was previously recorded in sweden. surprisingly, specific polymerase chain reaction analysis identified the protozoan as theileria annae. t. annae is endemic in northwest spain, is very uncommonly reported elsewhere and has never been recorded in s ...201323842507
efficacy of an imidacloprid/flumethrin collar against fleas and ticks on cats.the objectives of the studies listed here were to ascertain the therapeutic and sustained efficacy of 10% imidacloprid (w/w) and 4.5% flumethrin (w/w) incorporated in a slow-release matrix collar, against laboratory-infestations of fleas and ticks on cats. efficacy was evaluated against the flea ctenocephalides felis felis, and the ticks ixodes ricinus, amblyomma americanum and rhipicephalus turanicus. the number of studies was so large that only a general overview can be presented in this abstr ...201222541037
evaluation of the long-term efficacy and safety of an imidacloprid 10%/flumethrin 4.5% polymer matrix collar (seresto®) in dogs and cats naturally infested with fleas and/or ticks in multicentre clinical field studies in europe.the objective of these two gcp multicentre european clinical field studies was to evaluate the long-term efficacy and safety of a new imidacloprid/flumethrin collar (seresto®, bayer animalhealth, investigational veterinary product(ivp)) in dogs and cats naturally infested with fleas and/or ticks in comparison to a dimpylat collar ("ungezieferband fuer hunde/fuer katzen", beaphar, control product (cp)).201222463745
mitochondrial genomes suggest that hexapods and crustaceans are mutually paraphyletic.for over a century the relationships between the four major groups of the phylum arthropoda (chelicerata, crustacea, hexapoda and myriapoda) have been debated. recent molecular evidence has confirmed a close relationship between the crustacea and the hexapoda, and has included the suggestion of a paraphyletic hexapoda. to test this hypothesis we have sequenced the complete or near-complete mitochondrial genomes of three crustaceans (parhyale hawaiensis, squilla mantis and triops longicaudatus), ...016024395
complete mitochondrial genome sequence of urechis caupo, a representative of the phylum echiura.mitochondria contain small genomes that are physically separate from those of nuclei. their comparison serves as a model system for understanding the processes of genome evolution. although hundreds of these genome sequences have been reported, the taxonomic sampling is highly biased toward vertebrates and arthropods, with many whole phyla remaining unstudied. this is the first description of a complete mitochondrial genome sequence of a representative of the phylum echiura, that of the fat innk ...200415369601
single genotype of anaplasma phagocytophilum identified from ticks, camargue, france. 023697386
rickettsia species in ticks removed from humans in istanbul, turkey.a total of 167 ticks collected from humans in istanbul (turkey) in 2006 were screened for rickettsia species, and nested pcrs targeting glta and ompa rickettsial fragment genes were carried out. rickettsia monacensis (51), r. aeschlimannii (8), r. conorii subsp. conorii (3), r. helvetica (2), r. raoultii (1), r. africae (1), r. felis (1), and other rickettsia spp. (2), were detected. to our knowledge, these rickettsia species (except r. conorii) had never been reported in ticks removed from huma ...201222925016
the synergistic action of imidacloprid and flumethrin and their release kinetics from collars applied for ectoparasite control in dogs and cats.the control of tick and flea burdens in dogs and cats has become essential to the control of important and emerging vector borne diseases, some of which are zoonoses. flea worry and flea bite hypersensitivity are additionally a significant disease entity in dogs and cats. owner compliance in maintaining the pressure of control measures has been shown to be poor. for these reasons efforts are continuously being made to develop ectoparasiticides and application methods that are safe, effective and ...201222498105
rickettsia monacensis as cause of mediterranean spotted fever-like illness, italy. 022469314
seroprevalence and risk factors of ehrlichia canis infection among companion dogs of mashhad, north east of iran, 2009-2010.the aims of this study were to determine the seroprevalence of canine ehrlichiosis and risk factors of this disease in companion dogs' population of mashhad, north east of iran. canine monocytic ehrlichiosis (cme) is a zoonotic disease transmitted by ticks, rhipicephalus sanguineus, and caused by an obligate intracellular bacterium, ehrlichia canis.201526623430
repellency, prevention of attachment and acaricidal efficacy of a new combination of fipronil and permethrin against the main vector of canine babesiosis in europe, dermacentor reticulatus ticks.dermacentor reticulatus is a european hard tick of major veterinary importance because it is the vector of canine babesiosis due to babesia canis. the efficacy against this particular tick species is therefore a key characteristic for an acaricidal solution for dogs. the repellency, prevention of attachment and acaricidal efficacy of frontline tri- act/frontect, a new combination of fipronil and permethrin against induced infestations of dermacentor reticulatus ticks on dogs were evaluated after ...201525622802
a new multiple-locus variable-number tandem repeat analysis reveals different clusters for anaplasma phagocytophilum circulating in domestic and wild ruminants.anaplasma phagocytophilum is a tick-borne intragranulocytic alpha-proteobacterium. it is the causative agent of tick-borne fever in ruminants, and of human granulocytic anaplasmosis in humans, two diseases which are becoming increasingly recognized in europe and the usa. however, while several molecular typing tools have been developed over the last years, few of them are appropriate for in-depth exploration of the epidemiological cycle of this bacterium. therefore we have developed a multiple-l ...201425228371
the role of cats in the eco-epidemiology of spotted fever group diseases.mediterranean spotted fever (msf), whose etiological agent is r. conorii, is one of the oldest described vector-borne infectious diseases. although it is endemic in the mediterranean area, clinical cases have also been reported in other regions. r. massiliae-bar29 is related to msf cases. this strain is distributed worldwide. r. conorii and r. massiliae-bar29 are transmitted by ticks. dogs are considered the sentinel of r. conorii infection. cats could also be involved in their transmission. ric ...201425084969
frequency of piroplasms babesia microti and cytauxzoon felis in stray cats from northern italy.emerging diseases caused by piroplasms pose a health risk for man and other animals, and domestic cats have been proposed as potential reservoirs for some piroplasm infections. the aim of this study was to identify the frequency of the piroplasms babesia microti and cytauxzoon felis in stray cats from northern italy and to identify possible risk factors associated with these infections. blood samples from 260 stray cats enrolled in a trap-neuter-release (tnr) program in northern italy were exami ...201424895629
proteomic analysis of cattle tick rhipicephalus (boophilus) microplus saliva: a comparison between partially and fully engorged females.the cattle tick rhipicephalus (boophilus) microplus is one of the most harmful parasites affecting bovines. similarly to other hematophagous ectoparasites, r. microplus saliva contains a collection of bioactive compounds that inhibit host defenses against tick feeding activity. thus, the study of tick salivary components offers opportunities for the development of immunological based tick control methods and medicinal applications. so far, only a few proteins have been identified in cattle tick ...201424762651
abstracts of the 1st conference on neglected vectors and vector-borne diseases (eurnegvec): with management committee and working group meetings of the cost action td1303, april 8-11, 2014, cluj-napoca, romania. 201425078468
detection of vector-borne pathogens in cats and their ectoparasites in southern italy.vector-borne pathogens are the subject of several investigations due to the zoonotic concern of some of them. however, limited data are available about the simultaneous presence of these pathogens in cats and their ectoparasites. the aim of the present study was to define the species of ectoparasites found on cats as well as to investigate vector-borne pathogens in cats and their ectoparasites in southern italy.201627160725
evaluation of the in vitro expression of atp binding-cassette (abc) proteins in an ixodes ricinus cell line exposed to ivermectin.ticks are among the most important vectors of pathogens causing human and animal disease. acaricides are used to control tick infestation, although there are increasing reports of resistance. recently, over-expression of atp-binding cassette (abc) transporter proteins (p-glycoproteins, pgp) has been implicated in resistance to the acaricide ivermectin in the ticks rhipicephalus (boophilus) microplus and rhipicephalus sanguineus sensu lato. ixodid tick cell lines have been used to investigate dru ...201627090214
knock-down and speed of kill of a combination of fipronil and permethrin for the prevention of ctenocephalides felis flea infestation in dogs.a topical combination of fipronil + permethrin (frontline tri-act/frontect, merial) has recently been developed to control fleas, ticks, mosquitoes, sandflies and stable flies on dogs. two studies were conducted to assess its speed of kill and knock-down effect on ctenocephalides felis fleas. the combination was compared to either fipronil alone or to a combination of permethrin, dinotefuran, and pyriproxyfen,201626830931
fatal babesia canis canis infection in a splenectomized estonian dog.a previously splenectomized dog from estonia was presented with a sudden lack of appetite and discoloration of the urine. despite supportive therapy, its condition deteriorated dramatically during 1 day. severe thrombocytopenia and high numbers of protozoan hemoparasites were evident in blood smears, and the hematocrit dropped from 46 to 33 %. the dog was euthanized before specific antibabesial treatment was initiated. blood samples from the dog and from two other dogs in the same household test ...201626810086
assessment of the prophylactic speed of kill of frontline tri-act(®) against ticks (ixodes ricinus and rhipicephalus sanguineus) on dogs.the objective of the study was to assess the speed of kill of a single topical treatment with a combination of fipronil and permethrin (frontline tri-act(®)/frontect(®)) against experimental infestations of ixodes ricinus and rhipicephalus sanguineus ticks on dogs. in this parallel group designed, randomised, single centre, controlled efficacy study, 16 healthy adult dogs were allocated to two groups: 8 dogs were treated with the topical combination on day 0 and the other 8 dogs served as untrea ...201626795064
the first detection of rickettsia aeschlimannii and rickettsia massiliae in rhipicephalus turanicus ticks, in northwest china.rickettsia spp. belonging to the spotted fever group (sfg) cause infections in humans, domestic animals and wildlife. at least five sfg rickettsial species have been reported in china, but the occurrence of rickettsia aeschlimannii and r. massiliae in ticks has not been characterized to date.201526652857
targeted silencing of the aquaporin 2 gene of rhipicephalus (boophilus) microplus reduces tick fitness.ticks are blood-feeding arthropods that can affect human and animal health both directly by blood-feeding and indirectly by transmitting pathogens. the cattle tick rhipicephalus (boophilus) microplus is one of the most economically important ectoparasites of bovines worldwide and it is responsible for the transmission of the protozoan babesia bovis, the etiological agent of bovine babesiosis. aquaporins (aqps) are water channel proteins implicated in physiological mechanisms of osmoregulation. m ...201526626727
candidatus neoehrlichia sp. in an austrian fox is distinct from candidatus neoehrlichia mikurensis, but closer related to candidatus neoehrlichia lotoris.candidatus neoehrlichia came under the focus of recent research in terms of human and pet relevance. candidatus neoehrlichia mikurensis seems to be relatively abundant in animals and humans from central european countries, whereas candidatus neoehrlichia lotoris was found solely in raccoons from the usa.201526471191
repellency and acaricidal efficacy of a new combination of fipronil and permethrin against ixodes ricinus and rhipicephalus sanguineus ticks on dogs.a blinded, controlled laboratory study was conducted to assess the repellency and acaricidal activity of a topical spot on formulation, a combination of fipronil and permethrin, against ixodes ricinus and rhipicephalus sanguineus ticks on dogs.201526463998
ticks and associated pathogens collected from cats in sicily and calabria (italy).limited information is available about the species of ticks infesting the cat and the pathogens that they harbor. the aims of the present study were to identify the species of ticks removed from cats living in sicily and calabria (italy) and to detect dna of vector-borne pathogens in the same ticks.201526445916
piroplasmosis in wildlife: babesia and theileria affecting free-ranging ungulates and carnivores in the italian alps.piroplasmosis are among the most relevant diseases of domestic animals. babesia is emerging as cause of tick-borne zoonosis worldwide and free-living animals are reservoir hosts of several zoonotic babesia species. we investigated the epidemiology of babesia spp. and theileria spp. in wild ungulates and carnivores from northern italy to determine which of these apicomplexan species circulate in wildlife and their prevalence of infection.201424533742
survey of toxoplasma gondii and neospora caninum, haemotropic mycoplasmas and other arthropod-borne pathogens in cats from albania.albania is a country on the western part of the balkan peninsula. the mediterranean climate is favourable for the stable development of many arthropod species, which are incriminated as vectors for various agents. recently, several papers have reported on epidemiological aspects of parasitic diseases including vector-borne disease agents of dogs with zoonotic characteristics in albania. however, data on the epidemiology of feline parasitic and bacterial agents in albania is scarce.201424517118
multispacer typing of rickettsia isolates from humans and ticks in tunisia revealing new genotypes.rickettsioses are important remerging vector born infections. in tunisia, many species have been described in humans and vectors. genotyping is important for tracking pathogen movement between hosts and vectors. in this study, we characterized rickettsia species detected in patients and vectors using multispacer typing (mst), proposed by founier et al. and based on three intergenic spacers (dksa-xerc, rmpe- trna(fmet), mppa-pruc) sequencing.201324380581
molecular detection of vector-borne agents in dogs from ten provinces of china.although many vector-borne agents are potential zoonoses and cause substantial morbidity and mortality in dogs worldwide, there are limited data on these organisms in dogs of china.201526428085
anaplasma phagocytophilum infection in domestic animals in ten provinces/cities of china.a nationwide epidemiologic investigation of domestic animal infections has been conducted in nine provinces and one city during 2007-2010. serum samples from a total of 707 goats, 433 cattle, and 219 dogs were collected for detecting anaplasma phagocytophilum igg antibody by immunofluorescence assays and the average seroprevalences were 10.05% for dogs, 3.82% for goats, and 0.69% for cattle, respectively. a total of 472 goats, 201 cattle, 102 dog blood clots, and 1,580 ticks were collected for p ...022764312
rocky mountain spotted fever characterization and comparison to similar illnesses in a highly endemic area-arizona, 2002-2011.rocky mountain spotted fever (rmsf) has emerged as a significant cause of morbidity and mortality since 2002 on tribal lands in arizona. the explosive nature of this outbreak and the recognition of an unexpected tick vector, rhipicephalus sanguineus, prompted an investigation to characterize rmsf in this unique setting and compare rmsf cases to similar illnesses.201525697743
community-based control of the brown dog tick in a region with high rates of rocky mountain spotted fever, 2012-2013.rocky mountain spotted fever (rmsf) transmitted by the brown dog tick (rhipicephalus sanguineus sensu lato) has emerged as a significant public health risk on american indian reservations in eastern arizona. during 2003-2012, more than 250 rmsf cases and 19 deaths were documented among arizona's american indian population. the high case fatality rate makes community-level interventions aimed at rapid and sustained reduction of ticks urgent. beginning in 2012, a two year pilot integrated tick pre ...201425479289
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