| altering the surface properties of baculovirus autographa californica npv by insertional mutagenesis of the envelope protein gp64. | the envelope protein gp64 of the baculovirus autographa californica nuclear polyhedrosis virus is essential for viral entry into insect cells, as the glycoprotein both mediates ph-dependent membrane fusion and binds to host cell receptors. surface modification of baculovirus particles by genetic engineering of gp64 has been demonstrated by various strategies and thus has become an important and powerful tool in molecular biology. to improve further the presentation of peptides on the surface of ... | 2002 | 12230557 |
| expression of human bone morphogenetic protein 2 in bombyx mori larvae and its purification. | recombinant bm-bacpak-bmp2 virus which contains the human bone morphogenetic protein 2 (bmp2) cdna gene under the control of polyhedrin gene promoter was generated by cotransfection into bm-n cells with the transfer plasmid pbacpak-bmp2 and the modified bombyx mori nuclear polyhedrosis virus bm-bacpak genomic dna. on the fifth day after infection with the recombinant virus, bombyx mori larvae produced about 10 &mgr;g rbmp2 in 1 ml hemolymph. the expressed rbmp2 was processed into 16 kd c-termina ... | 1996 | 12232629 |
| sequencing and structural analysis of a novel early-late gene of the leucania separata nuclear polyhedrosis virus. | | 1996 | 12237712 |
| baculovirus infection raises the level of tata-binding protein that colocalizes with viral dna replication sites. | during the infection cycle of autographa californica multicapsid nuclear polyhedrosis virus, the tata-binding protein (tbp) of the insect host cell likely participates in early viral transcription, which is mediated by the host rna polymerase ii. however, the role of tbp in late and very late viral transcription, which is accomplished by an alpha-amanitin-resistant rna polymerase, is unclear. we observed a dramatic increase of tbp protein during the late phases of infection. tbp mrna levels, how ... | 2002 | 12368354 |
| insect cells as a factory to produce adeno-associated virus type 2 vectors. | recombinant adeno-associated viruses (raav) are produced transiently in mammalian cells usually by cotransfecting two or three plasmids containing aav genes, adenovirus helper genes, and a vector genome. expansion and transfection of adherent cells limit the scale of raav production. efficient transfection is performed with cells on solid support media such as tissue culture plates. a large animal study or a human clinical trial may require 10(15) particles of vector, depending on dose. to gener ... | 2002 | 12427305 |
| functional properties of a rainbow trout cyp3a27 expressed by recombinant baculovirus in insect cells. | cytochrome p450 3a27 (cyp3a27) is highly expressed in liver and intestine of rainbow trout (oncorhynchus mykiss). in many animal species, the intestine and liver are responsible for the first-pass metabolism of a wide range of xenobiotics. to help determine its physiological role, the catalytic capabilities of cyp3a27 protein were examined. an open reading frame of cyp3a27 in pfastbac donor plasmid was transferred to the baculovirus genome (bacmid dna) through tn7 site-specific transposition in ... | 2002 | 12433811 |
| expression of biologically active recombinant equine interferon-gamma by two different baculovirus gene expression systems using insect cells and silkworm larvae. | the full-length equine interferon-gamma (eifn-gamma) cdna, including the secretion signal peptide coding region, was recloned into baculovirus transfer vector pacym1. this vector was co-transfected with autographa californica nuclear polyhedrosis virus dna or hybrid nuclear polyhedrosis virus dna into spodoptera frugiperda cells. the recombinant viruses, named aceifn-gamma and hyeifn-gamma, were then recovered. recombinant eifn-gamma (reifn-gamma) was accumulated in the culture fluid of the acei ... | 2002 | 12445800 |
| use of an n-terminal half truncated ie1 as an antagonist of ie1, an essential regulatory protein in baculovirus. | an immediate-early gene product of baculovirus, ie1, is essential for viral gene expression and for viral dna replication. it has been demonstrated for autographa californica nuclear polyhedrosis virus (acnpv) that the c-terminal region of ie1 is required for dimerization. and the acidic n-terminal region of ie1 has been identified as the activation domain. we constructed an n-terminal 267 amino acid (a.a.) truncated mutant of bombyx mori nuclear polyhedrosis virus (bmnpv) ie1, which was defecti ... | 2002 | 12457979 |
| interaction of heliothis armigera nuclear polyhedrosis viral capsid protein with its host actin. | in order to find the cellular interaction factors of the heliothis armigera nuclear polyhedrosis virus capsid protein vp39, a heliothis armigera cell cdna library was constructed. then vp39 was used as bait. the host actin gene was isolated from the cdna library with the yeast two-hybrid system. this demonstrated that vp39 could interact with its host actin in yeast. in order to corroborate this interaction in vivo, the vp39 gene was fused with the green fluorescent protein gene in plasmid pegfp ... | 2002 | 12470589 |
| [biosynthesis of a single peptide chain containing human chorionic gonadotropin beta and c3d of complement]. | in view of the strong immunity-enhancing function of hel-c3d3 designed by dr. paul w. dempsey, we made our efforts to produce a similar recombinant protein of hcg beta. with polymerase chain reaction, we introduced a bam hi restriction site into the 3' terminal of hcg beta cdna. the new cdna and its terminal's correctness has been confirmed by sequencing. then we have it covalently attached to the c3d3 cdna at the pre-designed bam hi/bgl ii site. having the chimeric dna correctly cloned into the ... | 1999 | 12548792 |
| [analysis and expression of hyphantria cunea nuclear polyhedrosis virus sod gene]. | the sequencing results indicated that hyphantria cunea nuclear polyhedrosis virus (hcnpv) sod gene open reading frame of 456 nt encoding protein of 151 amino acid, was identified to that of bombyx mori nuclear polyhedrosis virus (bmnpv), and exhibited 97.2% homology at nucletde level to that of autographa californica nuclear polyhedrosis virus (acnpv), three amino acid residues difference in amino acid level with acnpv sod. the essential amino acid residues for the construction and active could ... | 2001 | 12549022 |
| [package of bombyx mori nuclear polyhedrosis virus with heterogoneous polyhedring protein]. | cloning the segment of polyhedrin gene which was separated from acmnpv genome dna by pcr method into transfer vector pbacpak8, we got recombinant transfer vector poac, then contransfecting bmn cells with the vector and linear virus bm-bacpak6, harvested recombinant virus hp-bmnpv which can produce polyhedrin but no blue plaques. analysing of recombinant virus from polyhedrin, recombinant viral dna and polyhedra, we confirmed that polyhedring of acmnpv can be not only high-level expressed in bmn ... | 2002 | 12557348 |
| baculovirus studies in new, indigenous lepidopteran cell lines. | eight lepidopteran cell lines were established recently and their susceptibility to different insect viruses was studied. two spodoptera litura cell lines from the larval and pupal ovaries, were found highly susceptible to s. litura nuclear polyhedrosis virus (slnpv, 5-6 x 10(6) npv/ml). the helicoverpa armigera cell line from the embryonic tissue was highly susceptible to h. armigera npv (hanpv, 6.3 x 10(6) npv/ml). these in vitro grown slnpv and hanpv caused 100% mortality to respective 2nd in ... | 2002 | 12561971 |
| [expression of schistosoma japonicun fatty acid binding protein gene in silkworm cells and larvae]. | to express the fatty acid binding protein (sj14fabp) gene of schistosoma japonicun in the silkworm cells and larvae. | 1999 | 12563767 |
| [application of nuclear polyhedrosis virus of insects in parasitology]. | | 2000 | 12567649 |
| mass production of polyhedral occlusion bodies of npv of helicoverpa armigera in relation to dose, age and larval weight. | a significant difference was noticed in the yield of polyhedral occlusion bodies (pobs) in various larval instars of h. armigera when three different doses of the nuclear polyhedrosis virus (npv) were administered. the yield of pobs from a single larva ranged from 0.35 x 10(6) to 25033.33 x 10(6) with a mean of 18422.33 x 10(6) for fourth instar inoculated. positive correlation existed between larval weight and number of pobs recovered. the regression analysis indicated pobs recovered responded ... | 2002 | 12597558 |
| expression of dopamine d1 receptor in sf9 insect cells and agonism of l-12-chloroscoulerine on recombinant d1 receptor. | to express dopamine d1 receptor in baculovirus-sf9 cell system, and to investigate the effects of l-12-chloroscoulerine (l-csl) on the recombinant d1 receptor (d1r). | 2003 | 12617770 |
| cloning, tissue distribution, and functional studies of a new cytochrome p450 3a subfamily member, cyp3a45, from rainbow trout (oncorhynchus mykiss) intestinal ceca. | in trout and mammals, the major extrahepatic expression site for cyp3a forms is in the intestine. a cdna encoding a new cyp3a subfamily member was isolated from rainbow trout intestinal ceca by reverse transcriptase-polymerase chain reaction (rt-pcr), followed by rapid amplification of cdna ends (race)-pcr. in a set of two primers for pcr, a consensus sequence in the highly conserved regions in 17 cyp3a sequences was used for one primer, and the second primer was designed based on adapter sequen ... | 2003 | 12646270 |
| large-scale production of porcine mature interleukin-18 (il-18) in silkworms using a hybrid baculovirus expression system. | in this report, a hybrid baculovirus expression system, which means a hybrid virus of the autographa californica nuclear polyhedrosis virus and the bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of porcine mature interleukin-18 (il-18) in silkworms. two recombinant hybrid baculoviruses containing cdna of the porcine precursor il-18 and the porcine caspase-1 were constructed and were used to infect silkworm larvae. after the co-infection of the two viruses, porcin ... | 2003 | 12655117 |
| molecular cloning and sequence analysis of the anticarsia gemmatalis multicapsid nuclear polyhedrosis virus gp64 glycoprotein. | the gp64 locus of anticarsia gemmatalis multicapsid nucleopolyhedrovirus isolate santa fe (agmnpv-sf) was characterised molecularly in our laboratory. to this end, we have located and cloned a agmnpv-sf genomic dna fragment containing the gp64 gene and sequenced the complete gp64 locus. nucleotide sequence analysis indicated that the agmnpv gp64 gene consists of a 1500 nucleotide open reading frame (orf), encoding a protein of 499 amino acids. of the seven gp64 homologues identified to date, the ... | 2003 | 12680694 |
| a baculovirus enhancin alters the permeability of a mucosal midgut peritrophic matrix from lepidopteran larvae. | the peritrophic matrix (pm) in lepidopterous larvae may function as a defensive barrier against ingested viral pathogens. pms isolated from trichoplusia ni and pseudaletia unipuncta larvae, were treated with a baculovirus-encoded metalloprotease (enhancin) from trichoplusia ni granulosis virus (tngv) and their in vitro permeability to blue dextran and fluorescent-labelled autographa californica nuclear polyhedrosis virus (acmnpv) was determined using a dual chamber permeability apparatus. incuba ... | 1999 | 12770384 |
| establishment and characterization of a continuous cell line from pupal ovaries of japanese oak silkworm antheraea yamamai guerin-meneville. | pupal ovaries of the wild oak silkworm antheraea yamamai guerin-meneville were cultured in mgm-448 (modified grace medium-448) medium containing 10% fetal bovine serum. after the primary culture was set up in 1988, a continuous cell line was obtained in 1991, designated as nises-anya-0611 (anya-0611). the population doubling time was 54 hrs. and 19 min. at 96 passages and 88 hrs. and 29 min. at 387 passages. spindle-shaped and spherical cells coexisted in the cell group. the cell line karyotype ... | 2003 | 12892519 |
| expression of vp2 gene protein of infectious bursal disease virus detected in korea. | the vp2 gene dna (1.4 kb in approximate) of a very virulent infectious bursal disease virus (vvibdv) chinju strain detected in chinju, korea was cloned into the bacmid, a baculovirus shuttle vector, through transposition of the gene from initially cloned pfastbachta plasmid, a baculovirus expression vector, and was subsequently expressed in spodoptera frugiperda (sf) cells. biological properties of the expressed vp2 subunit protein were characterized to aid in the development of genetically engi ... | 2003 | 12913360 |
| [connection of virus carriage and acute infection, caused by nuclear polyhedrosis virus in native populations of silkworm (lymantria dispar l., lym. lepid.)]. | the detection rate of dna of nuclear polyedrosis virus in the caterpillar organism ranged from 28.6 to 73.2%, the detection rate of virion antigens ranged from 10.3 to 38.2%, and that of the virus polyhedron antigens ranged from 16.1 to 52.3% in 4 natural populations of gipsy moths in the territory of the novosibirsk region at the outbreak culmination time of a mass reproduction of the insects. an artificial activation of virus reproduction, in experiment, caused death of the insects at a level ... | 2003 | 12924100 |
| rna interference as a metabolic engineering tool: potential for in vivo control of protein expression in an insect larval model. | many ex vivo factors influence the yield of recombinant protein produced via acmnpv (autographa californica multiple nucleocapsid nuclear polyhedrosis virus) in trichoplusia ni (t. ni) larvae. among these are: the method of infection, the time of infection, the virus load, and the time of harvest. in vivo strategies, however, that attempt to manipulate host function in this and other expression systems have largely been ignored. in this work, rna interference (rnai) is shown as an effective meta ... | 2003 | 12948752 |
| [influence of host age on the epizootic of nuclear polyhedrosis virus of spodoptera litura]. | through investigation of tracking infected spodoptera litura larvae, a study was carried out to observe the epizootic dynamics of nuclear virus disease of s. litura after virus release in different instar with concentration of 2.15 x 10(7) pibs.ml-1 in vegetable field. the results showed that with the age of s. litura increasing, the susceptibility to virus, mortality and death velocity decreased, the disease death sustained period of larvae obviously elongated, and the time that larval mortalit ... | 2003 | 12974004 |
| multiplication in vitro of a nuclear polyhedrosis virus in insect amoebocytes. | | 1961 | 13767303 |
| the effect of metamorphosis on nuclear-polyhedrosis virus infection in certain lepidoptera. | | 1965 | 14346127 |
| baculoviral polyhedrin as a novel fusion partner for formation of inclusion body in escherichia coli. | baculoviral polyhedrin, which originated from autographa californica nuclear polyhedrosis virus (acnpv), was employed for the first time as a novel fusion partner for expression of foreign proteins in an escherichia coli system. we characterized the expression of recombinant polyhedrin protein fused to green fluorescent protein (gfp). the polyhedrin fusion protein ( approximately 58 kda) was successfully expressed as an insoluble inclusion body comprising approximately 30% of the total cellular ... | 2003 | 14574705 |
| baculovirus capsid display: a novel tool for transduction imaging. | baculoviruses are enveloped insect viruses that can carry large quantities of foreign dna in their genome. baculoviruses have proved to be very promising gene therapy vectors but little is known about their transduction mechanisms in mammalian cells. we show in this study that autographa californica multiple nuclear polyhedrosis virus capsid is compatible with the incorporation of desired proteins in large quantities. fusions can be made to the n-terminus or c-terminus of the major capsid protei ... | 2003 | 14599820 |
| baculovirus expression system for magnetic sorting of infected cells and enhanced titer determination. | recombinant baculoviruses derived from the autographa californica nuclear polyhedrosis virus (acnpv) are widely used to express heterologous genes in insect cells, but the use of the baculovirus expression vector system (bevs) is hampered by slow and tedious procedures for the selection and separation of baculovirus-infected insect cells and for titer determination. here we developed a new technology based on the bicistronic vector with a fusion protein of the human integral plasma membrane glyc ... | 2004 | 14740489 |
| biological activity of recombinant bovine interferon tau using an autographa californica nuclear polyhedrosis virus expression system. | bovine interferon (bifn) tau, which plays a key role in maternal-fetal recognition of pregnancy, was expressed by an autographa californica nuclear polyhedrosis virus expression system. cdna coding bifntau was derived from cultured trophoblast cells. the recombinant (r) bifntau had high antiviral activity (1 x 10 (8) iu/mg) and the molecular weight of rbifntau was estimated to be 23 kda by western blotting analysis. we investigated the biological effect of rbifntau on prostaglandin (pg) f(2alpha ... | 2003 | 14967893 |
| increased mortality of gypsy moth lymantria dispar (l.) (lepidoptera: lymantriidae) exposed to gypsy moth nuclear polyhedrosis virus in combination with the phenolic gycoside salicin. | second instar gypsy moth, lymantria dispar (l.) (lepidoptera: lymantriidae), larvae suffered significantly greater mortality from aerially applied gypsy moth nuclear polyhedrosis virus (gypchek) when the virus was consumed on quaking aspen, populus tremuloides michx., versus red oak, quercus spp. l., foliage. laboratory assays in which various doses of gypchek and salicin (a phenolic glycoside present in aspen foliage) were tested in combination demonstrated that salicin significantly increased ... | 2003 | 14977101 |
| assessment of the application of baculoviruses for control of lepidoptera. | baculoviruses, among other insect viruses, are regarded as safe and selective bioinsecticides, restricted to invertebrates. they have been used worldwide against many insect pests, mainly lepidoptera. their application as microbial pesticides, however, has not met their potential to control pests in crops, forests, and pastures, with the exception of the nuclear polyhedrosis virus of the soybean caterpillar (anticarsia gemmatalis), which is used on approximately 1 million ha annually in brazil. ... | 1999 | 15012374 |
| [cooperative effect of spodoptera litura nuclear polyhedrosis virus with parasite wasp, microplitis sp. on the s. litura larvae]. | the relationship between the survival rate, developmental duration of microplitis sp. in spodoptera litura larvae and time of exposure of the host larvae to virus, the inoculation virus concentration were investigated to determined if parasites which emerged from virus-infected s. litura larvae had normal survive, and if the parasites could sever as the vector for s. litura nuclear polyhedrosis virus (sinpv). there was no significant difference in time that parasites spent in virus-infected larv ... | 2004 | 15146645 |
| characterization of two autographa californica nucleopolyhedrovirus proteins, ac145 and ac150, which affect oral infectivity in a host-dependent manner. | the genome of the baculovirus autographa californica nuclear polyhedrosis virus (acmnpv) contains two homologues, orf145 and orf150, of the heliothis armigera entomopoxvirus (haepv) 11,000-kda gene. polyclonal antibodies raised against the ac145 or ac150 protein were utilized to demonstrate that they are expressed from late to very late times of infection and are within the nuclei of infected sf-21 cells. transmission electron microscopy coupled with immunogold labeling of ac145 found this prote ... | 2004 | 15163737 |
| an additional copy of the homologous region (hr1) sequence in the autographa californica multinucleocapsid polyhedrosis virus genome promotes hyperexpression of foreign genes. | the autographa californica multinucleocapsid nuclear polyhedrosis virus genome contains nine homologous region (hr1, hr1a, hr2, hr2a, hr3, hr4a, hr4b, hr4c, and hr5) sequences that are thought to be involved in viral replication and activation of transcription. our results show that the 750 bp hr1 sequence is capable of functioning as an enhancer of transcription of foreign genes from the homologous late polyhderin gene promoter and the heterologous drosophila heat shock protein (hsp70) promoter ... | 2004 | 15209510 |
| [expression of human follicle stimulating hormone in insect cells]. | to study the expression of hfsh in insect cells, the cdna encoding the hfsh beta chain was cloned by overlapping-pcr using human chromosome dna extracted from placental tissue as template. then we constructed expression vector pvl1393/hfsh beta using an unfused protein nuclear polyhedrosis virus (acnpv) expression vector. the insect cells (sf9) were cotransfected with the expression vector and nuclear polyhedrosis linearized virus dna, and recombinant viruses acnpv-hfsh beta were collected. the ... | 2004 | 15259986 |
| baculovirus late expression factors. | autographa californica nuclear polyhedrosis virus, or acmnpv, is the type member of the baculoviruses, a family of double-stranded dna viruses with large circular genomes. the successive and concomitant expression of an assortment of early, late and very late genes is instrumental for successful baculovirus infection, and requires a switch from early dependence on a host cell-derived polymerase ii to a novel virus-encoded rna polymerase that is required for transcription later on in infection. a ... | 2004 | 15263813 |
| rapid parallel expression in e. coli and insect cells: analysis of five lef gene products of the autographa californica multiple nuclear polyhedrosis virus (acmnpv). | a number of strategies are emerging for the high throughput (htp) expression of recombinant proteins to enable structural and functional study. here we describe a workable htp strategy based on parallel protein expression in e. coli and insect cells. using this system we provide comparative expression data for five proteins derived from the autographa californica polyhedrosis virus genome that vary in amino acid composition and in molecular weight. although the proteins are part of a set of fact ... | 2004 | 15284479 |
| molecular structure of a proteolytic fragment of tlp20. | myosin light-chain kinase is responsible for the phosphorylation of myosin in smooth muscle cells. in some tissue types, the c-terminal portion of this large enzyme is expressed as an independent protein and has been given the name telokin. recently, an antibody directed against telokin was found to interact with a protein derived from the baculovirus autographa californica nuclear polyhedrosis virus. this protein was biochemically characterized and given the name tlp20 for telokin-like protein ... | 1996 | 15299576 |
| characterization of lactate dehydrogenase-elevating virus orf6 protein expressed by recombinant baculoviruses. | lactate dehydrogenase-elevating virus (ldv) has a strict species-specificity and can replicate only in a subset of mouse primary macrophages in vitro. because it is difficult to grow and purify sufficient quantities of ldv virions from the primary macrophages, it has been difficult to further characterize ldv envelope proteins. a few expression systems have been reported for structural analysis of the nonglycosylated envelope protein m/vp-2, however, very few studies of the antigenicity of m/vp- ... | 2004 | 15325515 |
| the murine thymic stromal lymphopoietin receptor is partially expressed with an extracellular c-terminus. | the gene encoding the murine thymic stromal lymphopoietin receptor was expressed in lepidopteran insect cells using the baculovirus expression vector system. the corresponding gene was inserted under the polyhedrin promoter of the autographa californica nuclear polyhedrosis virus and expressed with an n-terminal poly-histidine tag and a c-terminal flag-tag in the spodoptera frugiperda insect cell line sf9 during viral infection. flow cytometer analysis of cells infected with the produced recombi ... | 2005 | 15488943 |
| ha-vp39 binding to actin and the influence of f-actin on assembly of progeny virions. | we present evidence that actin is necessary for the successful assembly of hanpv virions. purified nucleocapsid protein ha-vp39 of heliothis armigera nuclear polyhedrosis virus (hanpv) was found to be able to bind to actin in vitro without assistance, as demonstrated by western blot and isothermal titration calorimeter. deltah and binding constants (k) detected by isothermal titration calorimeter strongly suggested that ha-vp39 first binds actin to seed the formation of hexamer complex of actin, ... | 2004 | 15503206 |
| promoter and piggybac activities within embryos of the potato tuber moth, phthorimaea operculella, zeller (lepidoptera: gelechiidae). | potato production in tropical and subtropical countries suffers from damage caused by the potato tuber moth (ptm), phthorimaea operculella. the aim of this research was the development of the components required for a germline transformation system for the ptm. we tested three components that are critical to genetic transformation systems for insects: promoter activity, marker gene expression, and transposable element function. we compared the transcriptional activities of five different promote ... | 2004 | 15527988 |
| efficient large-scale protein production of larvae and pupae of silkworm by bombyx mori nuclear polyhedrosis virus bacmid system. | silkworm is one of the most attractive hosts for large-scale production of eukaryotic proteins as well as recombinant baculoviruses for gene transfer to mammalian cells. the bacmid system of autographa californica nuclear polyhedrosis virus (acnpv) has already been established and widely used. however, the acnpv does not have a potential to infect silkworm. we developed the first practical bombyx mori nuclear polyhedrosis virus bacmid system directly applicable for the protein expression of silk ... | 2005 | 15596136 |
| expression and one-step purification of bovine interleukin-21 (il-21) in silkworms using a hybrid baculovirus expression system. | a hybrid baculovirus, a hybrid of the autographa californica nuclear polyhedrosis virus and the bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of bovine interleukin-21 (il-21) in silkworms. a recombinant hybrid baculovirus containing the full length of the cdna of bovine interleukin-21 was constructed and used to infect silkworm larvae or silkmoth pupae. after the infection of the virus, bovine mature il-21 was produced in the haemolymph or pupal cell lysates. a ... | 2004 | 15604780 |
| construction of a host range-expanded hybrid baculovirus of bmnpv and acnpv, and knockout of cysteinase gene for more efficient expression. | acnpv (autographa californica nuclear polyhedrosis virus) and bmnpv(bombyx mori nuclear polyhedrosis virus) are two principal insect-baculovirus expression systems, each having different characteristics. acnpv has a wider host range and can infect a series of cell lines thus making it suitable for cell suspension culture expression, but the small size of the host insect, a. californica, makes acnpv less suitable for large scale protein synthesis. in contrast, bmnpv can only infect the silkworm, ... | 2004 | 15623152 |
| [cloning and sequencing of p35 gene from bombyx mori nuclear polyhedrosis virus strain shandong]. | according to the 5' and 3' untranslated region sequences of p35 gene from bombyx mori nuclear polyhedrosis virus isolate t3, a pair of primers was designed employing computer analysis. with the primers,polymerase chain reaction (pcr) was performed and a 1080 bp fragment was obtained from bmnpv strain shandong. sequencing result showed that it was p35 gene (genbank accession number: ay157746), and it included an open reading frame of 900 bp, encoding 299 amino acids with mr=34.91 kda and pi=6.40. ... | 2003 | 15639959 |
| high-level expression of recombinant 3ab1 non-structural protein from fmdv in insect larvae. | for its potential usefulness in diagnosis, the non-structural protein 3ab1 from foot-and-mouth disease virus was expressed as a soluble protein by using autographa californica nuclear polyhedrosis virus as a vector. the 3ab1 coding sequence was introduced into acnpv genome via pbacpak3ab1 transfer vector to originate ac3ab1 recombinant baculovirus of phenotype occ-. rachiplusia nu larvae were injected with supernatants of sf9 cells infected with ac3ab1 and 5 days post-infection total protein ext ... | 2004 | 15664073 |
| involvement of the toll-like receptor 9 signaling pathway in the induction of innate immunity by baculovirus. | we have previously shown that mice inoculated intranasally with a wild-type baculovirus (autographa californica nuclear polyhedrosis virus [acnpv]) are protected from a lethal challenge by influenza virus. however, the precise mechanism of induction of this protective immune response by the acnpv treatment remained unclear. here we show that acnpv activates immune cells via the toll-like receptor 9 (tlr9)/myd88-dependent signaling pathway. the production of inflammatory cytokines was severely re ... | 2005 | 15709004 |
| construction of a transposon-mediated baculovirus vector hanpvid and a new cell line for expressing barnase. | in this study we developed the transposon-mediated shuttle vector 'hanpvid', which composed of hanpv (heliothis armigera nuclear polyhedrosis virus) genomic dna and a transposon cassette from bacmid of bac-to-bac system. hanpvid replicates in e. coli in the same way as bacmid and retains infective function in cotton bollworm cells (hz-am1). using hanpvid we constructed a recombinant virus, which could infect hz-am1 cells and generate recombinant hanpv (rha-bar) containing the barnase gene, a rib ... | 2005 | 15715945 |
| the function of envelope protein p74 from autographa californica multiple nucleopolyhedrovirus in primary infection to host. | this research investigated the function of envelope protein p74 of autographa californica multiple nuclear polyhedrosis virus (acmnpv) in primary infection to host. a p74-inactivation recombinant baculovirus, rac-gfp(delta) p74, was constructed by inserting gfp driven by acmnpv polyhedrin promoter into the p74 locus of acmnpv genome. bioassays showed that the p74-null occlusion bodies (obs) failed to infect its natural host larvae, spodoptera exigua, per os, while the p74-null budded virus (bvs) ... | 2005 | 15744572 |
| cloning and characterization of the g protein betagamma subunits from trichoplusia ni (high five cells). | baculoviral-mediated expression in insect cells has become a method of choice where high-level protein expression is desired and where expression in escherichia coliform (e. coli.) is unsuitable. genes of interest are inserted into the baculoviral genome of autographa californica nuclear polyhedrosis virus (acnpv) under the extremely strong, but very late polyhedron gene (polh). the preferred host lines are derived from spodoptera frugiperda (sf9 or sf21) or tricoplusia ni (high five, invitrogen ... | 2005 | 15763469 |
| microarray and rt-pcr screening for white spot syndrome virus immediate-early genes in cycloheximide-treated shrimp. | here, we report for the first time the successful use of cycloheximide (chx) as an inhibitor to block de novo viral protein synthesis during wssv (white spot syndrome virus) infection. sixty candidate ie (immediate-early) genes were identified using a global analysis microarray technique. rt-pcr showed that the genes corresponding to orf126, orf242 and orf418 in the taiwan isolate were consistently chx-insensitive, and these genes were designated ie1, ie2 and ie3, respectively. the sequences for ... | 2005 | 15780883 |
| baculoviral polyhedrin-bacillus thuringiensis toxin fusion protein: a protein-based bio-insecticide expressed in escherichia coli. | previously, we found that baculoviral polyhedrin (polh) used as a fusion partner for recombinant expression in escherichia coli showed almost the same characteristics (rapid solubilization under alkaline conditions and specific degradation by specific alkaline proteases in insect midgut) as the native baculoviral polh, and formed easily isolatable inclusion bodies. here, polh derived from the autographa californica nuclear polyhedrosis virus (acnpv) was fused with a bacillus thuringiensis (bt) t ... | 2005 | 15981278 |
| fossil evidence of insect pathogens. | the present report describes fossil evidence of insect pathogens, heretofore, almost non-existent, from six samples of amber ranging in age from 15 to 100 million years. they include a cytoplasmic polyhedrosis virus and trypanosomatid infection in an adult biting midge (diptera: ceratopogonidae), and a nuclear polyhedrosis virus in an adult sand fly (diptera: phlebotomidae), both from early cretaceous burmese amber, several types of fungal thalli on the cuticle of an adult mosquito (culicidae: d ... | 2005 | 16009374 |
| genetically engineered horseradish peroxidase for facilitated purification from baculovirus cultures by cation-exchange chromatography. | an engineered horseradish peroxidase isozyme c (hrp c) gene was constructed by the addition of a 6xarg fusion tail to 6xhis-hrp c by the pcr strategy. the 6xhis-6xarg-hrp c cdna was expressed in the sf9 insect cell line from spodoptera frugiperda infected with autographa californica nuclear polyhedrosis virus. the recombinant peroxidase isoelectric point was 9.5 as judged by isoelectric focusing and was purified directly from the culture medium at day-6 post-infection by cation-exchange chromato ... | 2005 | 16038999 |
| from metchnikoff to monsanto and beyond: the path of microbial control. | in 125 years since metchnikoff proposed the use of metarhizium anisopliae to control the wheat cockchafer and brought about the first field trials, microbial control has progressed from the application of naturalists' observations to biotechnology and precision delivery. this review highlights major milestones in its evolution and presents a perspective on its current direction. fungal pathogens, the most eye-catching agents, dominated the early period, but major mycological control efforts for ... | 2005 | 16039302 |
| expression of spider flagelliform silk protein in bombyx mori cell line by a novel bac-to-bac/bmnpv baculovirus expression system. | bombyx mori nuclear polyhedrosis virus (bmnpv) baculovirus expression system (bes) has a lot of advantages such as high expression efficiency, convenience, and low feeding cost. in this report, we used a recently developed bmnpv bacmid, which could infect both b. mori cell lines and silkworm larvae. the results showed it takes only 7 to 10 days to generate recombinant baculovirus and permit the rapid isolation from small-scale cultures and then use it to transfect b. mori cell lines, compared to ... | 2006 | 16158284 |
| preparation of chil-2 and ibdv vp2 fusion protein by baculovirus expression system. | this study aims to produce an effective subunit vaccine against infectious bursal disease virus (ibdv). the genes of chicken interleukin-2 (chil-2) and ibdv viral protein 2 (vp2) were amplified and fused by splice overlap extension-polymerase chain reaction (soe-pcr). the fusion gene was digested by ecor i/kpn i and inserted into pbacpak8 vector, resulting in recombinant transfer plasmid pbacpakvp2-il2. the recombinant plasmid was transfected into sf-9 cells accompanied with hybrid nuclear polyh ... | 2005 | 16212892 |
| insecticidal effects of buthus occitanus tunetanus botit6 toxin expressed in escherichia coli and baculovirus/insect cells. | botit6 is a neurotoxin polypeptide derived from the venom of the scorpion buthus occitanus tunetanus (bot). its mature form is composed of 62 amino acids. botit6 has been reported to be the most potent toxin from bot venom that has a strict selectivity for insects. such toxin may have potential as a potent animal-harmless tool against insects. using rt-pcr, we isolated and sequenced a cdna encoding 62 amino acid residues corresponding to the known amino acid sequence of botit6. we have expressed ... | 2005 | 16216259 |
| visualization of a recombinant gene protein in the baculovirus expression vector system using confocal scanning laser microscopy. | expression of the recombinant protein beta-galactosidase in the spodoptera frugiperda sf-9 insect cell line infected by the autographa californica nuclear polyhedrosis virus expressing beta-galactosidase (acnpv-betagal) was visualized using confocal scanning laser microscopy with fluorescent staining of both the recombinant protein and the cell nucleus. the average size of the insect cells and the intracellular dna concentration both increased markedly, respectively reading 3.8- and 2.3-fold the ... | 1999 | 16232550 |
| significant increase in recombinant protein production of a virus-infected sf-9 insect cell culture of low moi under low dissolved oxygen conditions. | spodoptera frugiperda sf-9 insect cells were infected with recombinant autographa californica nuclear polyhedrosis virus at a low multiplicity of infection (moi) (0.1), and the effect of dissolved oxygen (do) on the production of a polyhedrin promoter-driven recombinant protein (beta-galactosidase), intrinsic proteases (carboxyl and cysteine proteases), and the virus was determined. the do concentrations used in the present study were 45%, 25%, 5%, and 1.3% of air saturation. at 5% do the cell g ... | 2002 | 16233329 |
| transformed lepidopteran cells expressing a protein of the silkmoth fat body display enhanced susceptibility to baculovirus infection and produce high titers of budded virus in serum-free media. | baculovirus vectors constitute important tools for therapeutic protein production and mammalian cell transduction for gene therapy applications. a prerequisite for such applications is that the cell lines in which baculoviruses are propagated be maintained in serum-free media that are devoid of potential human pathogens. however, in serum-free media, the performance of baculovirus-based systems can be significantly reduced. in this report, we show that silkmoth-derived host cell lines for the bo ... | 2005 | 16233927 |
| development of a new high-throughput screening model for human high density lipoprotein receptor (cla-1) agonists. | to develop a new high-throughput screening model for human high-density lipoprotein (hdl) receptor (cd36 and limpii analogous-1, cla-1) agonists using cla-1-expressing insect cells. | 2005 | 16274109 |
| the use of a recombinant baculovirus expressing a chitinase from the hard tick haemaphysalis longicornis and its potential application as a bioacaricide for tick control. | baculoviruses are specific insect pathogens used as selective biological insecticides on lepidopteran insects. we have tested a recombinant baculovirus expressing a chitinase gene for its efficacy as a tick bioacaricide. the recombinant autographa californica multiple nuclear polyhedrosis virus expressing a chitinase enzyme (acmnpv-cht1) from the hard tick, haemaphysalis longicornis, was constructed and found to have a novel bioacaricidal effect against ticks. the recombinant baculovirus was use ... | 2006 | 16292677 |
| loss of posterior silk gland transcription specificity of fibroin light chain promoter due to absence of 41 bp sequence containing possible inhibitor binding sites. | the gene encoding fibroin light chain protein (fibl) is specifically expressed in the posterior silk gland of silkworm and repressed in other tissues. the binding sites of several transcription factors involved in the silk gland transcription specificity of fibl promoter have been recognized, including sgfb, psgf and bmfa. here we report the leak expression of the enhanced green fluorescent protein (egfp) reporter gene in tissues other than the posterior silk gland in vivo when under the control ... | 2005 | 16331326 |
| comparison of solid-phase radioimmunoassays for baculoviruses. | the sensitivity and cross-reaction of four solid-phase radioimmunoassays (ria) for trichoplusia ni nuclear polyhedrosis virus containing singly enveloped virions were investigated. the detection limits of each assay were as follows: indirect ria, 5 ng of dissolved polyhedron antigen; direct ria, 50 ng; indirect sandwich ria, 200 ng; and direct sandwich ria, 300 ng. the indirect and indirect sandwich rias showed considerable cross-reaction with other baculovirus antigens, but the direct and direc ... | 1978 | 16345305 |
| plaque assay of nuclear polyhedrosis viruses in cell culture. | the nuclear polyhedrosis virus of autographa californica has been titrated in spodoptera frugiperda cells by the plaque method, using a solid overlay which does not require either the use of modified culture medium or expensive purified agarose or the addition of culture medium as a liquid layer above the solid agarose. this assay is more sensitive than that using a viscous methyl cellulose overlay but less sensitive than the end-point dilution technique. neither trichoplusia ni nor bombyx mori ... | 1978 | 16345306 |
| physical factors that affect in vitro autographa californica nuclear polyhedrosis virus infection. | of the physical parameters tested for in vitro baculovirus infection, multiplicity of infection was most important in governing percent cell infection. most plaques formed within the first 5 min of incubation. efficiency of infection, however, was low, and the virus titer did not diminish during prolonged incubation. efficiency of infection improved markedly when cells or virus were preincubated with selected polyanions and polycations. precise regulation of the ph, osmotic pressure, and ionic c ... | 1981 | 16345768 |
| characterization of a nuclear polyhedrosis virus isolated from diseased gonometa podocarpi (lepidoptera:lasiocampidae). | gonometa podocarpi is an important pest of several species of pine in east africa, and large numbers of trees in plantations in kenya were partially or completely defoliated by the larval stage of this insect. after the infestation in the mt. elgon region, large numbers of dead and moribund larvae were found on the ground. examination of extracts of these larvae demonstrated the presence of an occluded virus. electron microscopy of purified sectioned polyhedra demonstrated the presence of virus ... | 1981 | 16345830 |
| two naturally occurring nuclear polyhedrosis virus variants of neodiprion sertifer geoffr. (hymenoptera; diprionidae). | two isolates of neodiprion sertifer (geoffr.) nuclear polyhedrosis virus from britain and north america were compared using three biochemical techniques. alkaline protease assays of polyhedra revealed the presence of endogenous enzyme activity in the british isolate but not in the north american isolate. sodium dodecyl sulfate polyacrylamide gel electrophoresis of virus particle structural polypeptides revealed only minor differences, with the exception that the north american virus was contamin ... | 1982 | 16345930 |
| generalized immunoassay for autographa californica nuclear polyhedrosis virus infectivity in vitro. | a quantitative in vitro immunoassay for the infectivity of autographa californica nuclear polyhedrosis virus was developed and performed in six different lepidopteran cell lines. the assay was not dependent upon cytopathic effect or polyhedron production, but rather upon viral antigen production and its recognition in a peroxidase-antiperoxidase staining procedure. the importance of using such an assay for accurately assessing infectivity in cell lines which produce polyhedra inefficiently was d ... | 1982 | 16346059 |
| dynamics of baculovirus growth and dispersal in mamestra brassicae l. (lepidopteranoctuidae) larval populations introduced into small cabbage plots. | the nuclear polyhedrosis virus of mamestra brassicae has been studied in larval populations of the moth introduced into small plots of cabbages. primary dispersal of virus from single foci of infected larvae resulted from enhanced movement of the larvae, which colonized new plants logarithmically. virus growth within the host population was quantified, and infection of young larvae in the following generation was related directly to the concentration of virus produced during the primary phase. s ... | 1983 | 16346197 |
| in vitro survey of autographa californica nuclear polyhedrosis virus interaction with nontarget vertebrate host cells. | thirty-five nontarget host cell lines, 23 of human and 12 of nonhuman vertebrate origin, were exposed to autographa californica nuclear polyhedrosis virus preparations derived from four different sources: polyhedra, hemolymph, cell culture medium, and cultured cells. the virus and cells were incubated together at two different temperatures, 28 or 37 degrees c, for four different lengths of time, 16, 40, 64, or 168 h, and the cells were assayed for the presence of virus by a peroxidase-antiperoxi ... | 1983 | 16346229 |
| characterization of gypsy moth (lymantria dispar) nuclear polyhedrosis virus. | characterization of the proteins and nucleic acid of the gypsy moth nuclear polyhedrosis virus isolated in ithaca, n.y. (ldnpv-it) is presented. a total of 29 viral structural proteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis when the virus was isolated in the absence of alkaline protease activity. fourteen surface envelope viral proteins were identified by lactoperoxidase iodination. eleven proteins were associated with nucleocapsids prepared by nonidet p-40 ... | 1983 | 16346355 |
| hydrophobic interactions involved in attachment of a baculovirus to hydrophobic surfaces. | the hydrophobic interactions of trichoplusia ni nuclear polyhedrosis virus were characterized by hydrophobic interaction chromatography. the determination of the hydrophobic force and some of the factors that influence its size is discussed in relation to the attachment to leaf surfaces of polyhedra during their use as biological control agents against insect pests. | 1986 | 16347113 |
| measurement of surface charge of baculovirus polyhedra. | the isoelectric points of three baculoviruses, trichoplusia ni nuclear polyhedrosis virus (npv), t. ni granulosis virus, and spodoptera littoralis npv were identified by cell electrophoresis. at neutral ph polyhedra were negatively charged. t. ni npv polyhedra were reacted with a number of reagents which could potentially attach to or degrade their surface structure. this gave information on the components that contribute to the charge profile of t. ni npv. this is discussed in relation to the u ... | 1987 | 16347308 |
| effects of temperature and ph on survival of free nuclear polyhedrosis virus of autographa californica. | the effects of temperature and low ph on replication and survival of nonoccluded autographa californica nuclear polyhedrosis virus were investigated. no virus replication or formation of polynuclear inclusion bodies occurred at 37 degrees c. the virus was immediately inactivated upon exposure to ph 2.0 and was inactivated within 1 h at ph 4.0. the virus titer slowly declined, a 3-orders of magnitude reduction in virus titer, at ph 5.0 during a 4-h exposure. virus survival at ph 6.0 was equal to ... | 1987 | 16347494 |
| comparative replication of lymantria dispar nuclear polyhedrosis virus strains in three continuous-culture cell lines. | we compared the replication of the gypsy moth (lymantria dispar) nuclear polyhedrosis virus in two new cell lines, from embryos and fat body of l. dispar, and in a previously available ovarian cell line. three virus isolates (the hamden strain [ldp-67] used commercially as gypchek, a plaque-purified clone of hamden [5-7d], and an isolate from abington, mass. [ab]) were each tested on the three cell lines. the fat-body-derived cell line proved best in terms of occlusion body production for all th ... | 1989 | 16347901 |
| risk assessment studies: detailed host range testing of wild-type cabbage moth, mamestra brassicae (lepidoptera: noctuidae), nuclear polyhedrosis virus. | the host range of a multiply enveloped nuclear polyhedrosis virus (npv) (baculoviridae) isolated from the cabbage moth, mamestra brassicae (lepidoptera: noctuidae), was determined by challenging a wide range of insect species with high (10 polyhedral inclusion bodies) and low (10 polyhedral inclusion bodies) doses of the virus. the identity of the progeny virus was confirmed by dot blotting. analysis of 50% lethal dose was carried out on selected species, and the progeny virus was identified by ... | 1990 | 16348279 |
| insecticidal activity of a bacterial crystal protein expressed by a recombinant baculovirus in insect cells. | baculoviruses are insect pathogens with a relatively slow speed of action, and this has limited their use as control agents of insect pests. introduction into baculoviruses of genes which code for proteins interfering specifically with insect metabolism or metamorphosis, such as toxins, hormones, and enzymes, may enhance the pathogenicity of these viruses. the complete insecticidal crystal protein gene cryia(b) of bacillus thuringiensis subsp. aizawai 7.21 was engineered into the nuclear polyhed ... | 1990 | 16348284 |
| co-occlusion and persistence of a baculovirus mutant lacking the polyhedrin gene. | a co-occlusion process was evaluated as a commercially and ecologically acceptable strategy for the development of genetically improved baculovirus insecticides. coinfection of spodoptera frugiperda (iplb-sf-21) tissue culture cells with autographa californica nuclear polyhedrosis virus (acmnpv) and an acmnpv mutant (ac-e10) lacking the polyhedrin gene resulted in occlusion of both virus types within polyhedra. the amount of occluded ac-e10 virions in progeny polyhedra populations during serial ... | 1990 | 16348313 |
| functional expression of lepidopteran-selective neurotoxin in baculovirus: potential for effective pest management. | recombinant baculovirus expressing insect-selective neurotoxins derived from venomous animals are considered as an attractive alternative to chemical insecticides for efficient insect control agents. recently we identified and characterized a novel lepidopteran-selective toxin, buthus tamulus insect-selective toxin (butait), having 37 amino acids and eight half cysteine residues from the venom of the south indian red scorpion, mesobuthus tamulus. the synthetic toxin gene containing the butait se ... | 2006 | 16406338 |
| in vivo analysis of fibroin heavy chain signal peptide of silkworm bombyx mori using recombinant baculovirus as vector. | in order to investigate the functional signal peptide of silkworm fibroin heavy chain (fibh) and the effect of n- and c-terminal parts of fibh on the secretion of fibh in vivo, n- and c-terminal segments of fibh gene were fused with enhanced green fluorescent protein (egfp) gene. the fused gene was then introduced into silkworm larvae and expressed in silk gland using recombinant acmnpv (autographa californica multiple nuclear polyhedrosis virus) as vector. the fluorescence of egfp was observed ... | 2006 | 16466694 |
| antibody-based surface plasmon resonance detection of intact viral pathogen. | surface plasmon resonance (spr) technique was used to directly detect an intact form of insect pathogen: the baculovirus, autographa californica multiple nuclear polyhedrosis virus (acmnpv). an spr sensor chip with three bio-functional layers was used to detect the intact acmnpv: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, protein a, and a mouse igg monoclonal antibody raised against a surface protein of the target viral pathogen. a two-channel (reference & tes ... | 2006 | 16470580 |
| construction of hybrib autographa californica nuclear polyhedrosis bacmid by modification of p143 helicase. | we developed a new hybrid nuclear polyhedrosis virus (npv) bacmid capable of infecting spodoptera frugiperda, tricoplusia ni, and bombyx mori, and b. mori cell lines for producing hybrid recombinant baculovirus that can carry a gene of interest and express it in a broad range of hosts. a gfp(uv)-beta1,3-n-acetylglucosaminyltransferase 2 fusion gene was expressed successfully in silkworm larvae using this hybrid bacmid. the hybrid npv bacmid provides an altogether simple and realistically feasibl ... | 2006 | 16478634 |
| baculovirus etl promoter acts as a shuttle promoter between insect cells and mammalian cells. | to identify a shuttle promoter that can mediate gene expression in both insect cells and mammalian cells to facilitate the development of a baculovirus vector-based mammalian cell gene delivery vehicle. | 2006 | 16490168 |
| in vivo production, stabilization, and infectivity of baculovirus preoccluded virions. | wild-type and polyhedrin-negative isolates of autographa californica nuclear polyhedrosis virus were replicated in fifth-instar trichoplusia ni larvae. insect tissues infected with wild-type virus contained two types of virions that are highly infectious when ingested, those occluded in polyhedra and preoccluded virions. tissue infected with the polyhedrin-negative virus contained only preoccluded virions. the relative potencies of the two types of infected tissue were determined by dose-mortali ... | 1996 | 16535202 |
| characterization of baculovirus autographa californica multiple nuclear polyhedrosis virus infection in mammalian cells. | the baculovirus autographa californica multiple nuclear polyhedrosis virus (acmnpv) is used as a vector in many gene therapy studies. wild-type acmnpv infects many mammalian cell types in vitro, but does not replicate. we investigated the dynamics of acmnpv genomic dna in infected mammalian cells and used flow cytometric analysis to demonstrate that recombinant baculovirus containing a cytomegalovirus immediate early promoter/enhancer with green fluorescent protein (gfp) expressed high levels of ... | 2006 | 16545777 |
| a novel method for in vitro radiolabeling and testing enveloped viruses by phosphatidylethanolamine n-methyltransferase and host cell-specific binding. | the present study developed a novel virus labeling and testing method, referred to as an envelope-labeled virus assay (elva), in which virus envelope is labeled in vitro by the action of phosphatidylethanolamine n-methyltransferase (pemt) and tested through a host cell-specific binding. a recombinant strain (vgfpuv) of autographa californica multiple nuclear polyhedrosis virus (acmnpv) and spodoptera frugiperda (sf-9) insect cells were used as a model of viruses and host cells, respectively. the ... | 2006 | 16552776 |
| expression of bioactive recombinant bovine interferon-gamma using baculovirus. | the precise role of bovine interferon-gamma (boifn-gamma) in disease and therapy is still poorly defined. clearly it is involved in defence against parasites, bacteria, viruses and possibly tumor cells. this paper reports the expression of boifn-gamma in a baculovirus system to generate a fully functional recombinant protein. bovine interferon-gamma cdna was cloned from mitogen stimulated peripheral blood mononuclear cell (pbmc) rna utilizing the reverse transcription-polymerase chain reaction ( ... | 2006 | 16608121 |
| enhancing insecticidal efficacy of baculovirus by early expressing an insect neurotoxin, lqhit2, in infected trichoplusia ni larvae. | lqhit(2), an insect specific neurotoxin from the venom of leiurus quinquestriatus hebraeus, has been demonstrated to improve insecticidal efficacy of autographa californica nuclear polyhedrosis virus (acmnpv). a polyhedrin-positive recombinant acmnpvvacp(hsp70)egfp/p(pag90)it(2) was engineered for larvae to express the enhanced green fluorescence protein (egfp) and lqhit(2) under the control of p(hsp70) and p(pag90) promoters, respectively. this would allow a visual observation of the viral infe ... | 2006 | 16636828 |
| cross infectivity of baculovirus, spilarctia obliqua nuclear polyhedrosis virus against mulberry pest, porthesia xanthorrhoea kollar. | spilarctia obliqua nucleopolyhedrosis virus (sonpv) has been found effective against s. obliqua (walker) as well as porthesia xanthorrhoea kollar. lc50 concentration of s. obliqua sonpv against s. obliqua and p. xanthorrhoea was 2.5 x 10(4) and 3.7 x 10(4) pobs/ml, respectively. lt50 of sonpv against s. obliqua was 5.73 days, while it was 6.98 days for p. xanthorrhoea. cross infectivity of sonpv against p. xanthorrhoea and ease of its mass production may make it an ideal biopesticide. | 2006 | 16708898 |
| improving baculovirus transduction of mammalian cells by surface display of a rgd-motif. | an rgd-containing peptide, comprising 23 amino acids from the foot-and-mouth disease virus (fmdv) vp1 protein was engineered into the envelope of autographa californica nuclear polyhedrosis virus surface (acnpv) using two different display strategies. the rgd-motif is a well-described tripeptide, that by binding to cell surface integrins facilitates virus entry into cells. this epitope was displayed, either by directly modifying the native major envelope protein gp64 of acnpv, or by incorporatin ... | 2006 | 16716431 |
| effects of cry1ac toxin of bacillus thuringiensis and nuclear polyhedrosis virus of helicoverpa armigera (hübner) (lepidoptera: noctuidae) on larval mortality and pupation. | in the laboratory, the percentage mortality and pupation of helicoverpa armigera (hübner) were investigated when larvae were exposed to cry1ac of bacillus thuringiensis berliner, nuclear polyhedrosis virus of h. armigera (hanpv) or cry1ac and hanpv together. the results revealed that interactions between cry1ac and hanpv varied with bioassay method and concentration of the suspension. when larvae were infected using a suspension containing both hanpv and cry1ac, most combinations of cry1ac (62.5 ... | 2006 | 16770833 |
| effect of alkaline protease on the antigenic nature of wiseana nuclear polyhedrosis virus polyhedron protein. | polyhedron protein from wiseana spp. nuclear polyhedrosis virus was found to be degraded by an alkali protease when polyhedra are dissolved in alkali. the protease activity did not occur at high ph (0.1 m naoh) and was inactivated by heating polyhedra to 70 degrees c for 3 h. the products from the protease degradation of wiseana spp. nuclear polyhedrosis virus polyhedron protein retain the antigenicity of undegraded polyhedron protein as measured by the direct solid-phase radioimmunoassay and im ... | 1977 | 16789167 |
| alkaline protease associated with virus particles of a nuclear polyhedrosis virus: assay, purification, and properties. | proteolytic activity was detected within polyhedra of the nuclear polyhedrosis virus of spodoptera littoralis. the enzyme activity was detected by its ability to degrade the major structural polypeptide of polyhedra (polyhedrin). a quantitative assessment of activity was made by a radioassay technique using (3)h-labeled polyhedrin as the substrate. of the structural components of polyhedra, virus particles showed the greatest specific proteolytic activity. preparations of purified nucleocapsids ... | 1978 | 16789169 |
| physical map of the dna genome of autographa californica nuclear polyhedrosis virus. | a physical map of the 88 x 10(6) dalton, circular dna genome of autographa californica nuclear polyhedrosis virus was constructed. the complete order of bamhi and xmai restriction enzyme sites was determined. the ecori and hindiii fragments were partially ordered, and their general locations, relative to the bamhi and xmai maps, were determined. alterations in the restriction endonuclease fragment patterns of natural genotypic variants of a. californica nuclear polyhedrosis virus, including tric ... | 1979 | 16789175 |