| separation of integument and nucellar tissues from cotton ovules (gossypium hirsutum l.) for both high- and low-throughput molecular applications. | here we present a quick and low-cost method to separate the different layers of tissue from the ovules and young seeds of cotton (gossypium hirsutum l.) for use in high- and low-throughput molecular applications. this method is performed at room temperature using standard laboratory equipment and does not require embedding of the samples, time-consuming fixation, or micro-sectioning procedures. we show that the three main tissues can be efficiently separated from isolated ovules collected on the ... | 2012 | 23237485 |
| growing season carbon dioxide exchange in flooded non-mulching and non-flooded mulching cotton. | there is much interest in the role that agricultural practices might play in sequestering carbon to help offset rising atmospheric co₂ concentrations. however, limited information exists regarding the potential for increased carbon sequestration of different management strategies. the objective of this study was to quantify and contrast carbon dioxide exchange in traditional non-mulching with flooding irrigation (tf) and plastic film mulching with drip irrigation (pm) cotton (gossypium hirsutum ... | 2012 | 23226376 |
| high-frequency regeneration via multiple shoot induction of an elite recalcitrant cotton (gossypium hirsutum l. cv narashima) by using embryo apex. | cotton (gossypium hirsutum l.) is one of the most commercially important fiber crops in the world. compared with other crops, cotton represents a recalcitrant species for regeneration protocols. the development of efficient and rapid regeneration protocol for elite indian cotton variety could help improve the quality characteristics and biotic or abiotic stress tolerance. here we report a novel regeneration protocol in indian cotton cultivar narashima. the maximum number of multiple shoots obtai ... | 2013 | 23221745 |
| protein differential expression in the elongating cotton (gossypium hirsutum l.) fiber under nitrogen stress. | nitrogen (n) is an essential macronutrient and an important factor limiting agricultural productivity. n deficient or excess conditions often occur during the cotton growth season and incorrect n application may affect cotton fiber yield and quality. here, the influence of n stress on the cotton fiber proteome was investigated by two-dimensional gel electrophoresis and mass spectrometry. the results indicated that n application rate affects nitrogen accumulation in fiber cells and fiber length. ... | 2012 | 23160829 |
| transcriptome profiling analysis reveals that flavonoid and ascorbate-glutathione cycle are important during anther development in upland cotton. | previous transcriptome profiling studies have investigated the molecular mechanisms of pollen and anther development, and identified many genes involved in these processes. however, only 51 anther ests of upland cotton (gossypium hirsutum) were found in ncbi and there have been no reports of transcriptome profiling analyzing anther development in upland cotton, a major fiber crop in the word. | 2012 | 23155472 |
| functional genomics of fuzzless-lintless mutant of gossypium hirsutum l. cv. mcu5 reveal key genes and pathways involved in cotton fibre initiation and elongation. | fuzzless-lintless cotton mutants are considered to be the ideal material to understand the molecular mechanisms involved in fibre cell development. although there are few reports on transcriptome and proteome analyses in cotton at fibre initiation and elongation stages, there is no comprehensive comparative transcriptome analysis of fibre-bearing and fuzzless-lintless cotton ovules covering fibre initiation to secondary cell wall (scw) synthesis stages. in the present study, a comparative transc ... | 2012 | 23151214 |
| agrobacterium-mediated transformation of cotton (gossypium hirsutum) shoot apex with a fungal phytase gene improves phosphorus acquisition. | cotton is an important world economic crop plant. it is considered that cotton is recalcitrant to in vitro proliferation. somatic embryogenesis and plant regeneration has been successful by using hypocotyl, whereas it is highly genotype dependent. here, a genotype-independent cotton regeneration protocol from shoot apices is presented. shoot apices from 3- to 5-day-old seedlings of cotton are infected with an agrobacterium strain, eha105, carrying the binary vector pc-ksa contained phytase gene ... | 2013 | 23143496 |
| investigating transgene integration and organization in cotton (gossypium hirsutum l.) genome. | in this chapter, we present detailed experimental procedures for investigating integration patterns of transgenes in cotton genome. we use conventional pcr and genomic southern blot hybridization to characterize integration of t-dna components and vector backbone fragments. for multiple copy insertions into the same site (complex loci), transgene/transgene junctions (including canonical and truncated t-dna and transgene involved vector backbone sequences) are characterized by pcr and sequencing. ... | 2013 | 23143486 |
| silicon carbide whisker-mediated transformation of cotton (gossypium hirsutum l.). | plant transformation methods are invaluable biotechnological tools to generate specific and targeted genetic variation for performance improvement of crop plants. genetic information is created by proper modification during gene cloning flanked by proper regulatory sequences and delivered to plants via -different plant transformation techniques. due to being a multipurpose plant, cotton has been subjected to different genetic transformation methods to provide the breeders with an opportunity to ... | 2013 | 23143485 |
| biolistic transformation of cotton embryogenic cell suspension cultures. | genetic transformation of cotton (gossypium hirsutum l.) is highly dependent on the ability to regenerate fertile plants from transgenic cells through somatic embryogenesis. induction of embryogenic cell cultures is genotype dependant. however, once embryogenic cell cultures are available, they can be effectively used for transformation by agrobacterium or biolistic bombardment methods. here i describe a detailed procedure to transform cotton embryogenic cell suspension cultures by biolistic bom ... | 2013 | 23143483 |
| biolistic transformation of cotton zygotic embryo meristem. | biolistic transformation of cotton (gossypium hirsutum l.) meristems, isolated from mature seed, is detailed in this report. a commercially available, helium-driven biolistic device (bio-rad pds1000/he) was used to bombard gold particles coated with a marker gene (uida or "β-glucuronidase") into the shoot meristem. the penetration of gold particles was dependent on bombardment parameters and it was mostly one to two cell layers deep. stable transformation of epidermal l1 layer was consistently o ... | 2013 | 23143482 |
| rnai-mediated ultra-low gossypol cottonseed trait: performance of transgenic lines under field conditions. | cottonseed remains a low-value by-product of lint production mainly due to the presence of toxic gossypol that makes it unfit for monogastrics. ultra-low gossypol cottonseed (ulgcs) lines were developed using rnai knockdown of δ-cadinene synthase gene(s) in gossypium hirsutum. the purpose of the current study was to assess the stability and specificity of the ulgcs trait and evaluate the agronomic performance of the transgenic lines. trials conducted over a period of 3 years show that the ulgcs ... | 2013 | 23078138 |
| extraction and characterization of nanocellulose structures from raw cotton linter. | this study aimed to characterize nanocellulose extracted from cotton (gossypium hirsutum) linters. the nanocellulose was subjected to electronic microscopy, thermal analysis, x-ray diffractometry, light scattering, and contact angle. the properties of the nanocellulose are considerably different from the linter. the acidic hydrolyses applied to extract the nanocrystals increased the crystallinity index and the hydrophilicity and decreased the thermal stability. on average, the nanocrystals were ... | 2013 | 23044127 |
| combining ability analysis for within-boll yield components in upland cotton (gossypium hirsutum l.). | cotton is an important cash crop worldwide, accounting for a large percentage of world agricultural exports; however, yield per acre is still poor in many countries, including pakistan. diallel mating system was used to identify parents for improving within-boll yield and fiber quality parameters. combining ability analysis was employed to obtain suitable parents for this purpose. the parental genotypes cp-15/2, niab krishma, cim-482, ms-39, and s-12 were crossed in complete diallel mating under ... | 2012 | 23007974 |
| global alteration of micrornas and transposon-derived small rnas in cotton (gossypium hirsutum) during cotton leafroll dwarf polerovirus (clrdv) infection. | small rnas (srnas) are a class of non-coding rnas ranging from 20- to 40-nucleotides (nts) that are present in most eukaryotic organisms. in plants, srnas are involved in the regulation of development, the maintenance of genome stability and the antiviral response. viruses, however, can interfere with and exploit the silencing-based regulatory networks, causing the deregulation of srnas, including small interfering rnas (sirnas) and micrornas (mirnas). to understand the impact of viral infection ... | 2012 | 22987114 |
| efficient decolorization and deproteinization using uniform polymer microspheres in the succinic acid biorefinery from bio-waste cotton (gossypium hirsutum l.) stalks. | bio-waste cotton (gossypium hirsutum l.) stalks were converted into succinic acid by simultaneous saccharification and fermentation (ssf) using actinobacillus succinogenes 130z. after 54 h ssf at 40 °c and ph 7.0, the production of succinic acid was 63 g/l, with 1.17 g/l/h productivity and 64% conversion yield. after ssf, a simple method for the decolorization and deproteinization of crude ssf broth was developed through adsorption tests of polystyrene (pst) microspheres. under optimized conditi ... | 2013 | 22985822 |
| mechanism of phytohormone involvement in feedback regulation of cotton leaf senescence induced by potassium deficiency. | to elucidate the phytohormonal basis of the feedback regulation of leaf senescence induced by potassium (k) deficiency in cotton (gossypium hirsutum l.), two cultivars contrasting in sensitivity to k deficiency were self- and reciprocally grafted hypocotyl-to-hypocotyl, using standard grafting (one scion grafted onto one rootstock), y grafting (two scions grafted onto one rootstock), and inverted y grafting (one scion grafted onto two rootstocks) at the seedling stage. k deficiency (0.03mm for s ... | 2012 | 22962680 |
| targeted capture of homoeologous coding and noncoding sequence in polyploid cotton. | targeted sequence capture is a promising technology in many areas in biology. these methods enable efficient and relatively inexpensive sequencing of hundreds to thousands of genes or genomic regions from many more individuals than is practical using whole-genome sequencing approaches. here, we demonstrate the feasibility of target enrichment using sequence capture in polyploid cotton. to capture and sequence both members of each gene pair (homeologs) of wild and domesticated gossypium hirsutum, ... | 2012 | 22908041 |
| molecular markers associated with the immature fiber (im) gene affecting the degree of fiber cell wall thickening in cotton (gossypium hirsutum l.). | cotton fiber fineness and maturity measured indirectly as micronaire (mic) are important properties of determining fiber grades in the textile market. to understand the genetic control and molecular mechanisms of fiber fineness and maturity, we studied two near isogenic lines, gossypium hirsutum, texas marker-1 wild type (tm-1) and immature fiber (im) mutant showing a significant difference in mic values. the fibers from im mutant plants were finer and less mature with lower mic values than thos ... | 2013 | 22890806 |
| a chimeric arabinogalactan protein promotes somatic embryogenesis in cotton cell culture. | arabinogalactan proteins (agps) are a family of extracellular plant proteoglycans implicated in many aspects of plant growth and development, including in vitro somatic embryogenesis (se). we found that specific agps were produced by cotton (gossypium hirsutum) calli undergoing se and that when these agps were isolated and incorporated into tissue culture medium, cotton se was promoted. when the agps were partly or fully deglycosylated, se-promoting activity was not diminished. testing of agps s ... | 2012 | 22858635 |
| identification and analyses of mirna genes in allotetraploid gossypium hirsutum fiber cells based on the sequenced diploid g. raimondii genome. | the plant genome possesses a large number of micrornas (mirnas) mainly 21-24 nucleotides in length. they play a vital role in regulation of target gene expression at various stages throughout the whole plant life cycle. here we sequenced and analyzed ≈ 10 million non-coding rnas (ncrnas) derived from fiber tissue of the allotetraploid cotton (gossypium hirsutum) 7 days post-anthesis using ncrna-seq technology. in terms of distinct reads, 24 nt ncrna is by far the dominant species, followed by 21 ... | 2012 | 22835981 |
| the removal of reactive black 5 from aqueous solutions by cotton seed shell. | this study investigated the removal of reactive black 5 (rb5) textile dye from aqueous solutions using cotton (gossypium hirsutum) seed shell (css) as low cost adsorbents. the data were described according to the freundlich and langmuir isotherm models. of these, the langmuir model provided the best fit for the experimental data. the highest measured adsorption density was 12.19 mg/g at ph 2. an equilibrium adsorption rate of rb5 by css (q(e) = 11.879 mg/g) was observed at 30 minutes. in order t ... | 2012 | 22834220 |
| sampling strategies for square and boll-feeding plant bugs (hemiptera: miridae) occurring on cotton. | sampling methods for square and boll-feeding plant bugs (hemiptera: miridae) occurring on cotton, gossypium hirsutum l., were compared with the intent to assess if one approach was viable for two species occurring from early-season squaring to late bloom in 25 fields located along the coastal cotton growing region of south texas. cotton fleaphopper, pseudatomoscelis seriatus (reuter), damages squares early-season and dominated collections using five sampling methods (approximately 99% of insects ... | 2012 | 22812128 |
| cotton gene expression profiles in resistant gossypium hirsutum cv. zhongzhimian kv1 responding to verticillium dahliae strain v991 infection. | verticillium wilt of cotton (gossypium hirsutum) is a widespread and destructive disease that is caused by the soil-borne fungus pathogen verticillium dahliae (v. dahliae). to study the molecular mechanism in wilt tolerance, suppression subtractive hybridization (ssh) and dot blot techniques were used to identify the specifically expressed genes in a superior wilt-resistant cotton cultivar (g. hirsutum cv. zhongzhimian kv1) after inoculation with pathogen. cdnas from the root tissues of zhongzhi ... | 2012 | 22733494 |
| molecular diversity, genomic constitution, and qtl mapping of fiber quality by mapped ssrs in introgression lines derived from gossypium hirsutum × g. darwinii watt. | because the genetic basis of current upland cotton cultivars is narrow, exploring new germplasm resources and discovering novel alleles relevant to important agronomic traits have become two of the most important themes in the field of cotton research. in this study, g. darwinii watt, a wild cotton species, was crossed with four upland cotton cultivars with desirable traits. a total of 105 introgression lines (ils) were successfully obtained. by using 310 mapped ssrs evenly distributed across th ... | 2012 | 22733444 |
| [monitoring and simulation of soil electrical conductivity based on the hyperspectral parameters of cotton (gossypium hirsutum) functional leaves]. | taking the salt-tolerant cotton variety ccri-44 and salt-sensitive cotton variety sumian 12 as test materials, a two-year pot experiment was conducted at the pailou experimental station of nanjing agricultural university in 2008 and 2009 to study the relationships of soil electrical conductivity (ec) with the spectral reflectance and hyperspectral indices of cotton functional leaves at different growth stages under five simulated salinity levels (0, 0.35%, 0.60%, 0.85%, and 1.00%) of coastal sal ... | 2012 | 22720615 |
| molecular characterization of an elicitor-responsive armadillo repeat gene (gharm) from cotton (gossypium hirsutum). | only a few armadillo (arm) repeat proteins have been characterized in plants where they appear to have diverse functions, including the regulation of defence responses. in this study, the identification, cloning and characterization of a gene, encoding an arm repeat protein (gharm), is described. gharm exists as multiple copies in cotton, with an 1713 bp orf encoding 570 amino acids. the predicted protein contains three consecutive arm repeats within an armadillo-type fold, with no other disting ... | 2012 | 22714909 |
| genome-wide identification of differentially expressed genes under water deficit stress in upland cotton (gossypium hirsutum l.). | cotton is the world's primary fiber crop and is a major agricultural commodity in over 30 countries. like many other global commodities, sustainable cotton production is challenged by restricted natural resources. in response to the anticipated increase of agricultural water demand, a major research direction involves developing crops that use less water or that use water more efficiently. in this study, our objective was to identify differentially expressed genes in response to water deficit st ... | 2012 | 22703539 |
| expression of atsap5 in cotton up-regulates putative stress-responsive genes and improves the tolerance to rapidly developing water deficit and moderate heat stress. | the regulation of gene expression is a key factor in plant acclimation to stress, and it is thought that manipulation of the expression of critical stress-responsive genes should ultimately provide increased protection against abiotic stress. the aim of this study was to test the hypothesis that the ectopic expression of the atsap5 (at3g12630) gene in transgenic cotton (gossypium hirsutum, cv. coker 312) will improve tolerance to drought and heat stress by up-regulating the expression of endogen ... | 2012 | 22633820 |
| overexpression of cotton (gossypium hirsutum) dirigent1 gene enhances lignification that blocks the spread of verticillium dahliae. | dirigent super-family abounds throughout the plant kingdom, especially vascular plants. to elucidate the function of cotton (gossypium hirsutum) dir genes in lignification, two cdnas (designated ghdir1 and ghdir2) encoding putative dirigent proteins were isolated from cotton cdna libraries. real-time quantitative reverse transcription-polymerase chain reaction analysis revealed that ghdir1 transcript was preferentially accumulated in cotton hypocotyls, whereas ghdir2 was predominantly expressed ... | 2012 | 22595512 |
| identification of rna editing sites in cotton (gossypium hirsutum) chloroplasts and editing events that affect secondary and three-dimensional protein structures. | rna editing can alter individual nucleotides in primary transcripts, which can cause the amino acids encoded by edited rna to deviate from the ones predicted from the dna template. we investigated rna editing sites of protein-coding genes from the chloroplast genome of cotton. fifty-four editing sites were identified in 27 transcripts, which is the highest editing frequency found until now in angiosperms. all these editing sites were c-to-u conversion, biased toward ndh genes and u_a context. ex ... | 2012 | 22576925 |
| cloning and expression analysis of novel aux/iaa family genes in gossypium hirsutum. | members of the aux/iaa gene family encode proteins that mediate the responses of auxin-regulated gene expression and regulate various aspects of plant morphological development. here, we provide the first identification and characterization of nine cdnas encoding the complete open reading frame (orf) of the aux/iaa family in cotton. these were designated ghaux1 to ghaux9 (gossypiumhirsutum aux/iaa). the proteins encoded by these nine genes had either whole or partially conserved domains of the a ... | 2012 | 22575728 |
| dominant gene cpls(r)1 corresponding to premature leaf senescence resistance in cotton (gossypium hirsutum l.). | cotton (gossypium hirsutum l.) premature leaf senescence-resistant inbred xlz33 and senescence-susceptible inbred lines xlz13 were selected and crossed to produce f(1), f(1)-reciprocal, f(2) and bc(1) generations for evaluation of leaf senescence process and inheritance. the results showed that leaf senescence processes for xlz13 and xlz33 were obviously different and leaf senescence traits could be distinguished between the two parents at particular periods of cotton growth. inheritance anlysis ... | 2012 | 22574717 |
| a novel mesh processing based technique for 3d plant analysis. | in recent years, imaging based, automated, non-invasive, and non-destructive high-throughput plant phenotyping platforms have become popular tools for plant biology, underpinning the field of plant phenomics. such platforms acquire and record large amounts of raw data that must be accurately and robustly calibrated, reconstructed, and analysed, requiring the development of sophisticated image understanding and quantification algorithms. the raw data can be processed in different ways, and the pa ... | 2012 | 22553969 |
| the cotton centromere contains a ty3-gypsy-like ltr retroelement. | the centromere is a repeat-rich structure essential for chromosome segregation; with the long-term aim of understanding centromere structure and function, we set out to identify cotton centromere sequences. to isolate centromere-associated sequences from cotton, (gossypium hirsutum) we surveyed tandem and dispersed repetitive dna in the genus. centromere-associated elements in other plants include tandem repeats and, in some cases, centromere-specific retroelements. examination of cotton genomic ... | 2012 | 22536361 |
| host preference of cotton fleahopper, pseudatomoscelis seriatus (reuter) is not labile to geographic origin and prior experience. | several phytophagous insects exhibit distinct preference for their host plants. in widely distributed generalist insects, host preference can be influenced by geographic variation in host plant distribution and abundance as well as by prior experience. we have studied host preference of the cotton fleahopper, pseudatomoscelis seriatus (reuter), a pest of cotton in texas and other neighboring states, by measuring olfactory orientation to horsemint (monarda punctata l.) and cotton (gossypium hirsu ... | 2012 | 22525067 |
| epidermal cell differentiation in cotton mediated by the homeodomain leucine zipper gene, ghhd-1. | gossypium hirsutum l. (cotton) fibres are specialized trichomes a few centimetres in length that grow from the seed coat. few genes directly involved in the differentiation of these epidermal cells have been identified. these include ghmyb25-like and ghmyb25, two related myb transcription factors that regulate fibre cell initiation and expansion. we have also identified a putative homeodomain leucine zipper (hd-zip) transcription factor, ghhd-1, expressed in trichomes and early fibres that might ... | 2012 | 22443311 |
| genome physical mapping of polyploids: a bibac physical map of cultivated tetraploid cotton, gossypium hirsutum l. | polyploids account for approximately 70% of flowering plants, including many field, horticulture and forage crops. cottons are a world-leading fiber and important oilseed crop, and a model species for study of plant polyploidization, cellulose biosynthesis and cell wall biogenesis. this study has addressed the concerns of physical mapping of polyploids with bacs and/or bibacs by constructing a physical map of the tetraploid cotton, gossypium hirsutum l. the physical map consists of 3,450 bibac c ... | 2012 | 22438974 |
| genotypic variations in photosynthetic and physiological adjustment to potassium deficiency in cotton (gossypium hirsutum). | a hydroponic culture experiment was conducted to determine genotypic variation in photosynthetic rate and the associated physiological changes in response to potassium (k) deficiency in cotton (gossypium hirsutum l.) seedlings with contrasting two cotton cultivars in k efficiency. the k-efficient liaomian18 produced 66.7% more biomass than the k-inefficient nucotn99(b) under k deficiency, despite their similar biomass under k sufficiency. compared with nucotn99(b), liaomian18 showed 19.4% higher ... | 2012 | 22387141 |
| a high-density simple sequence repeat and single nucleotide polymorphism genetic map of the tetraploid cotton genome. | genetic linkage maps play fundamental roles in understanding genome structure, explaining genome formation events during evolution, and discovering the genetic bases of important traits. a high-density cotton (gossypium spp.) genetic map was developed using representative sets of simple sequence repeat (ssr) and the first public set of single nucleotide polymorphism (snp) markers to genotype 186 recombinant inbred lines (rils) derived from an interspecific cross between gossypium hirsutum l. (tm ... | 2012 | 22384381 |
| spatial mapping of lipids at cellular resolution in embryos of cotton. | advances in mass spectrometry (ms) have made comprehensive lipidomics analysis of complex tissues relatively commonplace. these compositional analyses, although able to resolve hundreds of molecular species of lipids in single extracts, lose the original cellular context from which these lipids are derived. recently, high-resolution ms of individual lipid droplets from seed tissues indicated organelle-to-organelle variation in lipid composition, suggesting that heterogeneity of lipid distributio ... | 2012 | 22337917 |
| floral to green: mating switches moth olfactory coding and preference. | mating induces profound physiological changes in a wide range of insects, leading to behavioural adjustments to match the internal state of the animal. here, we show for the first time, to our knowledge, that a noctuid moth switches its olfactory response from food to egg-laying cues following mating. unmated females of the cotton leafworm (spodoptera littoralis) are strongly attracted to lilac flowers (syringa vulgaris). after mating, attraction to floral odour is abolished and the females fly ... | 2012 | 22319127 |
| development and mapping of snp assays in allotetraploid cotton. | a narrow germplasm base and a complex allotetraploid genome have made the discovery of single nucleotide polymorphism (snp) markers difficult in cotton (gossypium hirsutum). to generate sequence for snp discovery, we conducted a genome reduction experiment (ecori, bafi double digest, followed by adapter ligation, biotin-streptavidin purification, and agarose gel separation) on two accessions of g. hirsutum and two accessions of g. barbadense. from the genome reduction experiment, a total of 2.04 ... | 2012 | 22252442 |
| protein expression changes during cotton fiber elongation in response to low temperature stress. | low temperature stress is one of the major abiotic stresses limiting the formation of cotton (gossypium hirsutum l.) fiber qualities, especially fiber length. to investigate the molecular adaptation mechanisms of cotton fiber elongation to low temperature stress, two cotton cultivars, kemian 1 (low temperature-tolerant) and sumian 15 (low temperature-sensitive), were planted in the field at two sowing dates (25 april and 10 june). the two sowing dates resulted in different growing conditions and ... | 2012 | 22244703 |
| [gene mapping of an ultra-dwarf mutant in upland cotton]. | a ultra-dwarf mutant was newly found in upland cotton (gossypium hirsutum l.). this mutant was controlled by single recessive gene du. seventy pairs of polymorphic primers were selected from 1350 primers, which covered all the identified chromosomes by screening in parents and near-isogenic lines. gene du was mapped using an f2 population derived from intraspecific crosses between "ultra-dwarf 1" and "xinluzao 16". thirty-six primers were distributed in eight linkage groups, and du was linked to ... | 2011 | 22207386 |
| cloning and characterization of homeologous cellulose synthase catalytic subunit 2 genes from allotetraploid cotton (gossypium hirsutum l.). | cellulose synthase catalytic subunits (cesas) are the catalytic sites within a multisubunit complex for cellulose biosynthesis in plants. cesas have been extensively studied in diploid plants, but are not well characterized in polyploid plants. gossypium hirsutum is an allotetraploid cotton species producing over 90% of the world's cotton fibers. although g. hirsutum cesas (ghcesas) are responsible for cellulose production in cotton fiber, very limited numbers of ghcesa genes have been identifie ... | 2012 | 22200568 |
| generation of ests for flowering gene discovery and ssr marker development in upland cotton. | upland cotton, gossypium hirsutum l., is one of the world's most important economic crops. in the absence of the entire genomic sequence, a large number of expressed sequence tag (est) resources of upland cotton have been generated and used in several studies. however, information about the flower development of this species is rare. | 2011 | 22163052 |
| difference in mirna expression profiles between two cotton cultivars with distinct salt sensitivity. | micrornas (mirnas) are a class of endogenous, non-coding small rnas that play important roles in many developmental processes and stress responses in plants and animals. cotton (gossypium hirsutum l.) is considered a relatively salt-tolerant non-halophytic plant species. to study the role of mirnas in salt adaptation, a salt-tolerant cotton cultivar sn-011 and a salt-sensitive cultivar lm-6 were used to detect differentially expressed mirnas. using mirna microarray analysis and a computational a ... | 2012 | 22160515 |
| cotton shoot plays a major role in mediating senescence induced by potassium deficiency. | the objective of this study was to determine the roles of shoot and root in the regulation of premature leaf senescence induced by potassium (k) deficiency in cotton (gossypium hirsutum l.). two contrasting cultivars (ccri41, more sensitive to k deficiency; and scrc22, a less sensitive cultivar) were selected for self- and reciprocal-grafting, using standard grafting (one scion/one rootstock), y grafting (two scions/one rootstock) and inverted y grafting (one scion/two rootstocks) at the seedlin ... | 2012 | 22154601 |
| [salt tolerance evaluation of cotton (gossypium hirsutum) at its germinating and seedling stages and selection of related indices]. | a sand culture experiment was conducted to study the salt tolerance of 13 cotton cultivars at their germinating and seedling stages under the stress of different concentration nacl, and a cluster analysis was made on the salt tolerance, according to the subjection values of salt toxicity coefficients of multi-indices and the sum subjection value. it was observed that the appropriate concentration of nacl for the evaluation of salt tolerance was 150 mmol x l(-1). the salt tolerance differed with ... | 2011 | 22097366 |
| cdna-aflp-based genetical genomics in cotton fibers. | genetical genomics, or genetic analysis applied to gene expression data, has not been widely used in plants. we used quantitative cdna-aflp to monitor the variation in the expression level of cotton fiber transcripts among a population of inter-specific gossypium hirsutum × g. barbadense recombinant inbred lines (rils). two key fiber developmental stages, elongation (10 days post anthesis, dpa), and secondary cell wall thickening (22 dpa), were studied. normalized intensity ratios of 3,263 and 1 ... | 2012 | 22080217 |
| genetic and epigenetic status of triple exotic consanguinity cotton introgression lines. | introgression lines are some of the most important germplasm for breeding applications and other research conducted on cotton crops. the dna methylation level among 10 introgression lines of cotton (gossypium hirsutum) and three exotic parental species (g. arboreum, g. thurberi and g. barbadense) were assessed by methylation-sensitive amplified polymorphism (msap) technology. the methylation level in the introgression lines ranged from 33.3 to 51.5%. however, the lines pd0111 and pd0113 had the ... | 2011 | 21968805 |
| a combined functional and structural genomics approach identified an est-ssr marker with complete linkage to the ligon lintless-2 genetic locus in cotton (gossypium hirsutum l.). | cotton fiber length is an important quality attribute to the textile industry and longer fibers can be more efficiently spun into yarns to produce superior fabrics. there is typically a negative correlation between yield and fiber quality traits such as length. an understanding of the regulatory mechanisms controlling fiber length can potentially provide a valuable tool for cotton breeders to improve fiber length while maintaining high yields. the cotton (gossypium hirsutum l.) fiber mutation li ... | 2011 | 21902843 |
| genomically biased accumulation of seed storage proteins in allopolyploid cotton. | allopolyploidy is an important process during plant evolution that results in the reunion of two divergent genomes into a common nucleus. many of the immediate as well as longer-term genomic and epigenetic responses to polyploidy have become appreciated. to investigate the modifications of gene expression at the proteome level caused by allopolyploid formation, we conducted a comparative analysis of cotton seed proteomes from the allopolyploid gossypium hirsutum (ad genome) and its model a-genom ... | 2011 | 21900265 |
| recent long-distance transgene flow into wild populations conforms to historical patterns of gene flow in cotton (gossypium hirsutum) at its centre of origin. | over 95% of the currently cultivated cotton was domesticated from gossypium hirsutum, which originated and diversified in mexico. demographic and genetic studies of this species at its centre of origin and diversification are lacking, although they are critical for cotton conservation and breeding. we investigated the actual and potential distribution of wild cotton populations, as well as the contribution of historical and recent gene flow in shaping cotton genetic diversity and structure. we e ... | 2011 | 21899621 |
| evidence of female-produced sex pheromone of adelphocoris suturalis (hemiptera: miridae): effect of age and time of day. | after the reduction in the use of broad-spectrum insecticides, adelphocoris suturalis (hemiptera: miridae) has become an important pest of transgenic cotton, gossypium hirsutum l., in china. a. suturalis is a highly mobile insect, moving rapidly in and out of crop fields, and traps baited with sex pheromones may be useful in monitoring and controlling its numbers. however, little is currently known regarding the chemical communication based on sex pheromones by this species. to use the synthesiz ... | 2011 | 21882682 |
| transcriptome-wide identification and stress properties of the 14-3-3 gene family in cotton (gossypium hirsutum l.). | 14-3-3s are a class of conserved proteins in eukaryotes involved in almost all cellular processes. published studies have shown that this family plays a role in response to stress conditions. in this study, comparative genomics identified thirty-one 14-3-3 cdnas encoding 25 unique proteins with transcripts that were sufficiently divergent in the coding regions to define different 14-3-3 members. phylogenetic analysis indicated that the cotton 14-3-3 family was similar to the family in the plant ... | 2011 | 21805362 |
| a novel isoform of sucrose synthase is targeted to the cell wall during secondary cell wall synthesis in cotton fiber. | sucrose (suc) synthase (sus) is the major enzyme of suc breakdown for cellulose biosynthesis in cotton (gossypium hirsutum) fiber, an important source of fiber for the textile industry. this study examines the tissue-specific expression, relative abundance, and temporal expression of various sus transcripts and proteins present in cotton. a novel isoform of sus (susc) is identified that is expressed at high levels during secondary cell wall synthesis in fiber and is present in the cell wall frac ... | 2011 | 21757635 |
| qtl alleles for improved fiber quality from a wild hawaiian cotton, gossypium tomentosum. | seventeen backcross-self families from crosses between two gossypium hirsutum recurrent parent lines (ca3084, ca3093) and g. tomentosum were used to identify quantitative trait loci (qtls) controlling fiber quality traits. a total of 28 qtls for fiber quality traits were identified (p < 0.001), including four for fiber elongation, eight for fiber fineness, four for fiber length, four for fiber strength, six for fiber uniformity, one for boll weight, and one for boll number. three statistically s ... | 2011 | 21735234 |
| characterization of profilin genes from allotetraploid (gossypium hirsutum) cotton and its diploid progenitors and expression analysis in cotton genotypes differing in fiber characteristics. | the actin-binding protein profilin (prf) plays an important role in cell growth and expansion by regulating the organization of the actin filaments. recent studies have reported association between fiber elongation in cultivated cotton (gossypium hirsutum) and prf expression. in the present study, we cloned four genomic clones from allotetraploid cotton (g. hirsutum) and its putative diploid progenitors (g. arboreum and g. raimondii) designated ghprf1_a, ghprf1_d, gaprf1, and grprf1 encoding cot ... | 2012 | 21725637 |
| assessment of impact of solar uv components on growth and antioxidant enzyme activity in cotton plant. | a field experiment was conducted to study the impact of solar uv-b (280-315 nm) and uv-a (315-400 nm) components on the growth and antioxidant enzyme activity in cotton plant (gossypium hirsutum var. vikram). solar uv components were excluded by filtering the sunlight through selective uv-b (<315 nm) and uv-b/a (<400 nm) absorbing polyester films. plants grown under filters that transmitted solar uv served as controls. exclusion of uv-b and uv-b/a enhanced plant height, leaf area and total bioma ... | 2011 | 23573013 |
| elucidation of thermotolerance diversity in cotton (gossypium hirsutum l.) using physio-molecular approaches. | cotton (gossypium hirsutum) is an important cash crop, but high temperature during its growing season is one of the major factors that limit its productivity. this problem compels plant breeders to breed for heat tolerance, which can help to overcome this challenge. it is very important to make a comprehensive screening of heat-tolerant genotypes so that only the best are chosen. here we report the combined use of several techniques that can help breeders to screen their germplasm. twelve cultiv ... | 2011 | 21710467 |
| identification of exotic genetic components and dna methylation pattern analysis of three cotton introgression lines from gossypium bickii. | the impact of alien dna fragments on plant genome has been studied in many species. however, little is known about the introgression lines of gossypium. to study the consequences of introgression in gossypium, we investigated 2000 genomic and 800 epigenetic sites in three typical cotton introgression lines, as well as their cultivar (gossypium hirsutum) and wild parents (gossypium bickii), by amplified fragment length polymorphism (aflp) and methylation-sensitive amplified polymorphism (msap). t ... | 2013 | 21630566 |
| transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton (gossypium hirsutum l.). | high salinity is one of the main factors limiting cotton growth and productivity. the genes that regulate salt stress in tm-1 upland cotton were monitored using microarray and real-time pcr (rt-pcr) with samples taken from roots. microarray analysis showed that 1503 probe sets were up-regulated and 1490 probe sets were down-regulated in plants exposed for 3h to 100mm nacl, and rt-pcr analysis validated 42 relevant/related genes. the distribution of enriched gene ontology terms showed such import ... | 2011 | 21569837 |
| differential gene expression and associated qtl mapping for cotton yield based on a cdna-aflp transcriptome map in an immortalized f2. | cdna-aflp techniques have found new applications in recent years. currently, the methodology is used to establish differential gene expression and construct linkage maps. in the present study, a transcriptome map based on cdna-aflp techniques was constructed using an immortalized f(2) (if(2)) population of 171 lines. the lines were derived from intercrosses between 180 recombinant inbred lines (rils) of the cotton hybrid xiangzamian 2 (gossypium. hirsutum l.). a total of 302 transcriptome-derive ... | 2011 | 21512772 |
| identification of salt responsive genes using comparative microarray analysis in upland cotton (gossypium hirsutum l.). | salinity negatively impacts plant growth and productivity, and little is known about salt responsive genes in cotton. in this study, an intra-specific backcross population of cotton (gossypium hirsutum l.) was treated with 200 mm nacl after which differentially expressed genes were identified by comparison between salt tolerant and susceptible segregant bulks using comparative microarray analysis. microarray analysis identified 720 salt-responsive genes, of which 695 were down-regulated and only ... | 2011 | 21421393 |
| functional analysis of gossypium hirsutum cellulose synthase catalytic subunit 4 promoter in transgenic arabidopsis and cotton tissues. | gossypium hirsutum cellulose synthase catalytic subunit 4 (ghcesa4) plays an important role in cellulose biosynthesis during cotton fiber development. the transcript levels of ghcesa4 are significantly up-regulated as secondary cell wall cellulose is produced in developing cotton fibers. to understand the molecular mechanisms involved in transcriptional regulation of ghcesa4, β-glucuronidase (gus) activity regulated by a ghcesa4 promoter (-2574/+56) or progressively deleted promoters were determ ... | 2011 | 21421377 |
| differential contributions to the transcriptome of duplicated genes in response to abiotic stresses in natural and synthetic polyploids. | polyploidy has occurred throughout plant evolution and can result in considerable changes to gene expression when it takes place and over evolutionary time. little is known about the effects of abiotic stress conditions on duplicate gene expression patterns in polyploid plants. we examined the expression patterns of 60 duplicated genes in leaves, roots and cotyledons of allotetraploid gossypium hirsutum in response to five abiotic stress treatments (heat, cold, drought, high salt and water subme ... | 2011 | 21361962 |
| agronomic characterization, genetic diversity and association analysis of cotton cultivars using simple sequence repeat molecular markers. | cotton is the most important textile plant in the world and is one of the most important crops for the production of oilseed. because of its worldwide economic importance, new cultivars are constantly being released in the world and consequently in the greek market, as greece is the largest producer in europe. we used simple sequence repeat (ssr) markers for the identification and the phylogenetic analysis of the most widely cultivated cotton cultivars in greece. initially, we used 12 pairs of s ... | 2011 | 21341213 |
| development and population growth of lygus hesperus on selected weed hosts, artificial diet and cotton in the laboratory. | laboratory studies were conducted to obtain basic biological information of lygus hesperus knight (hemiptera: miridae) on alfalfa (medicago sativa l.), pigweed (amaranthus palmeri s. wats.), russian thistle (salsola iberica sennen and pau), green bean (phaseolus vulgaris l.), artificial diet, and cotton (gossypium hirsutum l.) square and cotton boll at 27 degrees c. the nymphal developmental duration was 28.8 d on cotton bolls, nearly twice longer, compared with approximately 15 d in other hosts ... | 2010 | 21309220 |
| contradictions in host plant resistance to pests: spider mite (tetranychus urticae koch) behaviour undermines the potential resistance of smooth-leaved cotton (gossypium hirsutum l.). | two-spotted spider mites (tetranychus urticae koch) oviposit near leaf veins or in leaf folds on the undersides of cotton (gossypium hirsutum l.) leaves where the humid boundary layer offers protection from desiccation. the authors predicted that the boundary layer of glabrous cotton leaves should be shallower than that of hairy leaves, providing some resistance to mites. the dynamics of mite populations, leaf damage, leaf gas exchange and crop yield on two leaf hair isolines (smooth versus hair ... | 2011 | 21308962 |
| delineation of interspecific epistasis on fiber quality traits in gossypium hirsutum by adaa analysis of intermated g. barbadense chromosome substitution lines. | genetic diversity is the foundation of any crop improvement program, but the most cultivated upland cotton [gossypium hirsutum l., 2n = 52, genomic formula 2(ad)(1)] has a very narrow gene pool resulting from its evolutionary origin and domestication history. cultivars of this cotton species (g. hirsutum l.) are prized for their combination of exceptional yield, other agronomic traits, and good fiber properties, whereas the other cultivated 52-chromosome species, g. barbadense l. [2n = 52, genom ... | 2011 | 21301803 |
| a mixture toxicity approach for environmental risk assessment of multiple insect resistance genes. | multiple substance considerations applied to chemical mixtures in ecological risk assessments can be logically extended to nontarget organism (nto) risk assessment for pyramided trait crops expressing multiple insect resistance genes. a case instance is developed that considers a two-protein pyramid of cry1f and cry1ac synthetic proteins expressed in cotton (gossypium hirsutum l.). a mixture toxicity approach was used to arrive at the aggregated multisubstance potentially affected fraction (mspa ... | 2011 | 21298718 |
| high temperature limits in vivo pollen tube growth rates by altering diurnal carbohydrate balance in field-grown gossypium hirsutum pistils. | it has recently been reported that high temperature slows in vivo pollen tube growth rates in gossypium hirsutum pistils under field conditions. although numerous physical and biochemical pollen-pistil interactions are necessary for in vivo pollen tube growth to occur, studies investigating the influence of heat-induced changes in pistil biochemistry on in vivo pollen tube growth rates are lacking. we hypothesized that high temperature would alter diurnal pistil biochemistry and that pollen tube ... | 2011 | 21256621 |
| genome structure of cotton revealed by a genome-wide ssr genetic map constructed from a bc1 population between gossypium hirsutum and g. barbadense. | cotton, with a large genome, is an important crop throughout the world. a high-density genetic linkage map is the prerequisite for cotton genetics and breeding. a genetic map based on simple polymerase chain reaction markers will be efficient for marker-assisted breeding in cotton, and markers from transcribed sequences have more chance to target genes related to traits. to construct a genome-wide, functional marker-based genetic linkage map in cotton, we isolated and mapped expressed sequence t ... | 2011 | 21214949 |
| cytogenetic characteristics of new monosomic stocks of cotton (gossypium hirsutum l.). | the use of aneuploid lines significantly increases the effectiveness of molecular-genetic analysis and the development of superior quality breeding lines via substitutions by alien chromosomes. to date, however, a complete set of aneuploid series for each cotton chromosome is not available. here, we present the development of a monosomic stock collection of cotton (gossypium hirsutum l.) from uzbekistan, including the origin of 92 primary monosomics, meiotic metaphase-i analysis, study of tetrad ... | 2011 | 23533784 |
| programmed cell death during pigment gland formation in gossypium hirsutum leaves. | ultrastructural studies have shown that the formation of pigment glands in gossypium hirsutum l. leaves is a lysigenous process, originating from a cluster of cells in the ground meristem. various techniques were used here to investigate whether programmed cell death (pcd) plays a critical role in this developmental process. nuclei of internal cells in the pigment gland-forming tissue were tunel-positive and dapi-negative, suggesting that dna cleavage is an early event and complete dna degradati ... | 2010 | 21040304 |
| [characterization of the damage of spodoptera eridania (cramer) and spodoptera cosmioides (walker) (lepidoptera: noctuidae) to structures of cotton plants]. | the cotton plant, gossypium hirsutum, hosts various pests that damage different structures. among these pests, spodoptera cosmioides (walker) and spodoptera eridania (cramer) (lepidoptera: noctuidae) are considered important. the objectives of this study were to characterize and to quantify the potential damage of s. eridania and s. cosmioides feeding on different structures of cotton plants. for this purpose, newly-hatched larvae were reared on the following plant parts: leaf and flower bud; le ... | 2010 | 20878002 |
| association of aflp and ssr markers with agronomic and fibre quality traits in gossypium hirsutum l. | molecular markers linked to qtl contributing to agronomic and fibre quality traits would be useful for cotton improvement. we have attempted to tag yield and fibre quality traits with aflp and ssr markers using f(2) and f(3) populations of a cross between two gossypium hirsutum varieties, ps56-4 and rs2013. out of 50 aflp primer combinations and 177 ssr primer pairs tested, 32 aflp and four ssr primers were chosen for genotyping f(2) individuals. marker-trait associations were studied for eight ... | 2010 | 20861566 |
| two cotton cys2/his2-type zinc-finger proteins, ghdi19-1 and ghdi19-2, are involved in plant response to salt/drought stress and abscisic acid signaling. | cotton (gossypium hirsutum) often encounters abiotic stress such as drought and high salinity during its development, and its productivity is significantly limited by those adverse factors. to investigate the molecular adaptation mechanisms of this plant species to abiotic stress, we identified two genes encoding di19-like cys2/his2 zinc-finger proteins in cotton. gfp fluorescence assay demonstrated that ghdi19-1 and ghdi19-2 are two nuclear-localized proteins. quantitative rt-pcr and northern b ... | 2010 | 20852918 |
| diurnal pollen tube growth rate is slowed by high temperature in field-grown gossypium hirsutum pistils. | for gossypium hirsutum pollination, germination, and pollen tube growth must occur in a highly concerted fashion on the day of flowering for fertilization to occur. because reproductive success could be influenced by the photosynthetic activity of major source leaves, we hypothesized that increased temperatures under field conditions would limit fertilization by inhibiting diurnal pollen tube growth through the style and decreasing subtending leaf photosynthesis. to address this hypothesis, g. h ... | 2011 | 20832140 |
| transcriptome profiling of early developing cotton fiber by deep-sequencing reveals significantly differential expression of genes in a fuzzless/lintless mutant. | cotton fiber as a single-celled trichome is a biological model system for studying cell differentiation and elongation. however, the complexity of its gene expression and regulatory mechanism allows only marginal progress. here, we report the high-throughput tag-sequencing (tag-seq) analysis using solexa genome analyzer platform on transcriptome of -2 to 1 (fiber initiation, stage i) and 2-8 (fiber elongation, stage ii) days post anthesis (dpa) cotton (gossypium hirsutum) ovules (wild type: wt; ... | 2010 | 20828606 |
| the history and disposition of transposable elements in polyploid gossypium. | transposable elements (tes) are a major component of plant genomes. it is of particular interest to explore the potential activation of te proliferation, especially in hybrids and polyploids, which often are associated with rapid genomic and epigenetic restructuring. here we explore the consequences of genomic merger and doubling on copia and gypsy-like gorge3 long terminal repeat (ltr) retrotransposons as well as on non-ltr long interspersed nuclear elements (lines) in allotetraploid cotton, go ... | 2010 | 20725147 |
| comparisons of early transcriptome responses to low-oxygen environments in three dicotyledonous plant species. | waterlogging is a serious impediment to crop productivity worldwide which acts to reduce oxygen levels in the rhizosphere due to the low diffusion rate of molecular oxygen in water. plants respond to low oxygen through rapid and specific changes at both the transcriptional and translational levels. transcriptional changes to low-oxygen (hypoxia) stress have been studied in a number of plant species using whole genome microarrays. using transcriptome data from root tissue from early time points ( ... | 2010 | 20724824 |
| identification of the family of aquaporin genes and their expression in upland cotton (gossypium hirsutum l.). | cotton (gossypium spp.) is produced in over 30 countries and represents the most important natural fiber in the world. one of the primary factors affecting both the quantity and quality of cotton production is water. a major facilitator of water movement through cell membranes of cotton and other plants are the aquaporin proteins. aquaporin proteins are present as diverse forms in plants, where they function as transport systems for water and other small molecules. the plant aquaporins belong to ... | 2010 | 20626869 |
| meta-analysis of cotton fiber quality qtls across diverse environments in a gossypium hirsutum x g. barbadense ril population. | cotton fibers (produced by gossypium species) are the premier natural fibers for textile production. the two tetraploid species, g. barbadense (gb) and g. hirsutum (gh), differ significantly in their fiber properties, the former having much longer, finer and stronger fibers that are highly prized. a better understanding of the genetics and underlying biological causes of these differences will aid further improvement of cotton quality through breeding and biotechnology. we evaluated an inter-spe ... | 2010 | 20584292 |
| overexpression of a profilin (ghpfn2) promotes the progression of developmental phases in cotton fibers. | cotton fiber development at the stages of elongation and secondary wall synthesis determines the traits of fiber length and strength. to date, the mechanisms controlling the progression of these two phases remain elusive. in this work, the function of a fiber-preferential actin-binding protein (ghpfn2) was characterized by cytological and molecular studies on the fibers of transgenic green-colored cotton (gossypium hirsutum) through three successive generations. overexpression of ghpfn2 caused p ... | 2010 | 20558432 |
| [determination of protein and gossypol content in cotton kernel powder with near infrared reflectance spectroscopy]. | near-infrared reflectance spectroscopy (nirs) was used as a rapid and nondestructive method to determine the protein content and gossypol content in cotton kernel powder samples, using 49 upland cotton (gossypium hirsutum l.) germplasms and 188 recombinant inbred lines (rils). the cottonseed samples harvested from the upland cotton germplasms and rils grown in different cotton growing regions in different years were analyzed chemically for protein and gossypol contents, as well as scanned in the ... | 2010 | 20496676 |
| cold acclimation and low temperature resistance in cotton: gossypium hirsutum phospholipase dalpha isoforms are differentially regulated by temperature and light. | phospholipase dalpha (pldalpha) was isolated from cultivated cotton (gossypium hirsutum) and characterized. two pldalpha genes were identified in the allotetraploid genome of g. hirsutum, derived from its diploid progenitors, g. raimondii and g. arboreum. the genes contained three exons and two introns. the translated products shared a 98.6% homology and were designated as grpldalpha and gapldalpha. their orfs encoded a polypeptide of 807 amino acids with a predicted molecular mass of 91.6 kda s ... | 2010 | 20478966 |
| identification and evaluation of new reference genes in gossypium hirsutum for accurate normalization of real-time quantitative rt-pcr data. | normalizing through reference genes, or housekeeping genes, can make more accurate and reliable results from reverse transcription real-time quantitative polymerase chain reaction (qpcr). recent studies have shown that no single housekeeping gene is universal for all experiments. thus, suitable reference genes should be the first step of any qpcr analysis. only a few studies on the identification of housekeeping gene have been carried on plants. therefore qpcr studies on important crops such as ... | 2010 | 20302670 |
| characterization of two cotton (gossypium hirsutum l) invertase genes. | two cotton vacuolar-invertase genes were identified and sequenced. both genes had seven exons, including an unusually small second exon typical of acid invertases. these genes encode peptides with many features shared by acid invertases from other species including, leader sequences that probably target the peptide to the vacuole, active site motifs and substrate binding motifs. expression analyses indicated that one of the genes was expressed in roots during the starch filling stage of developm ... | 2010 | 20300865 |
| characterization of an adp-glucose pyrophosphorylase small subunit gene expressed in developing cotton (gossypium hirsutum) fibers. | adp-glucose pyrophosphorylase (adpgp, ec 2.7.7.27) is a tetrameric protein composed of two small and two large subunits that catalyzes the biosynthesis of adp-glucose from glucose-phosphate which is used to provide the glucose subunits for starch biosynthesis. a second cotton gene encoding an adpgp small subunit has been cloned and characterized. the gene contains eight introns similar to previously reported potato and cotton adpgp small subunit genes. the deduced translation of the gene contain ... | 2011 | 20140709 |
| near-isogenic cotton germplasm lines that differ in fiber-bundle strength have temporal differences in fiber gene expression patterns as revealed by comparative high-throughput profiling. | gene expression profiles of developing cotton (gossypium hirsutum l.) fibers from two near-isogenic lines (nils) that differ in fiber-bundle strength, short-fiber content, and in fewer than two genetic loci were compared using an oligonucleotide microarray. fiber gene expression was compared at five time points spanning fiber elongation and secondary cell wall (scw) biosynthesis. fiber samples were collected from field plots in a randomized, complete block design, with three spatially distinct b ... | 2010 | 20087569 |
| cotton chromosome substitution lines crossed with cultivars: genetic model evaluation and seed trait analyses. | seed from upland cotton, gossypium hirsutum l., provides a desirable and important nutrition profile. in this study, several seed traits (protein content, oil content, seed hull fiber content, seed index, seed volume, embryo percentage) for f(3) hybrids of 13 cotton chromosome substitution lines crossed with five elite cultivars over four environments were evaluated. oil and protein were expressed both as percentage of total seed weight and as an index which is the grams of product/100 seeds. an ... | 2010 | 20087566 |
| tarnished plant bug (hemiptera: miridae) thresholds for cotton before bloom in the midsouth of the united states. | insecticide applications to control tarnished plant bug, lygus lineolaris (palisot de beauvois) (hemiptera: miridae), during cotton, gossypium hirsutum l., bud formation are common throughout the midsouth of the united states. cultivation practices and the pest complex have changed since action thresholds were established for this pest. a trial was conducted at 33 locations over 3 yr throughout the midsouth to evaluate tarnished plant bug damage to cotton during the prebloom period. there was no ... | 2009 | 20069839 |
| long-term effects of land application of class b biosolids on the soil microbial populations, pathogens, and activity. | this study evaluated the influence of 20 annual land applications of class b biosolids on the soil microbial community. the potential benefits and hazards of land application were evaluated by analysis of surface soil samples collected following the 20th land application of biosolids. the study was initiated in 1986 at the university of arizona marana agricultural center, 21 miles north of tucson, az. the final application of biosolids was in march 2005, followed by growth of cotton (gossypium h ... | 2010 | 20048328 |
| genotypic differences in thermotolerance are dependent upon prestress capacity for antioxidant protection of the photosynthetic apparatus in gossypium hirsutum. | numerous studies have illustrated the need for antioxidant enzymes in acquired photosynthetic thermotolerance, but information on their possible role in promoting innate thermotolerance is lacking. we investigated the hypothesis that genotypic differences in source leaf photosynthetic thermostability would be dependent upon prestress capacity for antioxidant protection of the photosynthetic apparatus in gossypium hirsutum. to test this hypothesis, thermosensitive (cv. st4554) and reportedly ther ... | 2010 | 20002327 |
| a new snp haplotype associated with blue disease resistance gene in cotton (gossypium hirsutum l.). | resistance to cotton blue disease (cbd) was evaluated in 364 f(2.3) families of three populations derived from resistant variety 'delta opal'. the cbd resistance in 'delta opal' was controlled by one single dominant gene designated cbd. two simple sequence repeat (ssr) markers were identified as linked to cbd by bulked segregant analysis. cbd resides at the telomere region of chromosome 10. ssr marker dc20027 was 0.75 cm away from cbd. dc20027 marker fragments amplified from 3 diploid species an ... | 2010 | 19960336 |
| proteomic identification of differentially expressed proteins in the ligon lintless mutant of upland cotton (gossypium hirsutum l.). | cotton fiber is an ideal model for studying plant cell elongation. to date, the underlying mechanisms controlling fiber elongation remain unclear due to their high complexity. in this study, a comparative proteomic analysis between a short-lint fiber mutant (ligon lintless, li(1)) and its wild-type was performed to identify fiber elongation-related proteins. by 2-de combined with local est database-assisted ms/ms analysis, 81 differentially expressed proteins assigned to different functional cat ... | 2010 | 19954254 |
| response of the enzymes to nitrogen applications in cotton fiber (gossypium hirsutum l.) and their relationships with fiber strength. | to investigate the response of key enzymes to nitrogen (n) rates in cotton fiber and its relationship with fiber strength, experiments were conducted in 2005 and 2006 with cotton cultivars in nanjing. three n rates 0, 240 and 480 kgn/hm(2), signifying optimum and excessive nitrogen application levels were applied. the activities and the gene expressions of the key enzymes were affected by n, and the characteristics of cellulose accumulation and fiber strength changed as the n rate varied. beta-1 ... | 2009 | 19937205 |