| the structure of the specific capsular polysaccharide of rhodococcus equi serotype 4. | the specific capsular polysaccharide produced by rhodococcus equi serotype 4 was found to be a high-molecular-weight acidic polymer composed of d-glucose, d-mannose, pyruvic acid and a previously unidentified 5-amino-3,5-dideoxynonulosonic (rhodaminic) acid in the proportions 2:1:1:1. structural analysis, employing a combination of microanalytical methods, nuclear magnetic resonance spectroscopy, and mass spectrometric techniques, established that the polysaccharide consisted of linear repeating ... | 1999 | 10573859 |
| in vitro activity of moxifloxacin, a new 8-methoxyquinolone, against gram-positive bacteria. | the in vitro activity of moxifloxacin, formerly bay 12-8039, against gram-positive bacteria was tested by the agar dilution method. a total of 189 isolates that included staphylococcus aureus, enterococcus faecalis, enterococcus faecium, streptococci, rhodococci, leuconostocs, pediococci, lactobacilli, and diphtheroids were tested. moxifloxacin showed greater potency than ciprofloxacin against s. aureus, streptococci, and enterococci, having minimal inhibitory concentrations (mics) lower than th ... | 1999 | 10579093 |
| rhodopeptins (mer-n1033), novel cyclic tetrapeptides with antifungal activity from rhodococcus sp. i. taxonomy, fermentation, isolation, physico-chemical properties and biological activities. | five novel cyclic tetrapeptides, named rhodopeptin c1, c2, c3, c4 and b5, were isolated from a strain named rhodococcus sp. mer-n1033. they are a novel type of cyclic tetrapeptide composed of a beta-amino acid and three usual alpha-amino acids. rhodopeptins show high in vitro antifungal activity against candida albicans and cryptococcus neoformans, whereas they show no activity against bacteria. | 1999 | 10580382 |
| rhodopeptins, novel cyclic tetrapeptides with antifungal activities from rhodococcus sp. ii. structure elucidation. | the structures of rhodopeptins, novel antifungal peptides, were determined on the basis of physico-chemical analyses of the intact molecules and their acid hydrolysates. the structures of rhodopeptins c1, c2, c3, c4 and b5 were determined to be cyclo (-gly-l-orn-l-val-3-amino-10-methyldodecanoyl-), cyclo (-gly-l-orn-l-ile-3-amino-10-methyldodecanoyl-), cyclo (-gly-l-orn-l-val-3-amino-12-methyltridecanoyl-), cyclo (-gly-l-orn-l-val-3-amino- 12-methyltetradecanoyl-) and cyclo (-gly-l-lys-l-val-3-a ... | 1999 | 10580383 |
| rhodopeptins, novel cyclic tetrapeptides with antifungal activities from rhodococcus sp. iii. synthetic study of rhodopeptins. | total syntheses of cyclo (-gly-l-lys-l-val-(r)-3-aminododecanoyl-); lv9na and its diastereomer cyclo (-gly-l-lys-l-val-(s)-3-aminododecanoyl-); lv9nb, congeners of rhodopeptin b5 on beta-amino acid moiety, were achieved. the beta-amino acid moiety was prepared as a racemate by the thermal michael addition of an amine to alpha,beta-unsaturated ester. the racemic beta-amino acids were converted to their l-valylamide derivatives and the obtained diastereomers were separated. coupling of both diaste ... | 1999 | 10580384 |
| bacterial degradation of hydrocarbons as evidenced by respirometric analysis. | the microbial biodegradability of mineral oil and other hydrocarbons, namely hexane, decane and tetradecane was determined using the warburg constant volume respirometer. results of oxygen uptake indicated that hexane and tetradecane were more degradable than mineral oil and decane. rhodococcus erythropolis and erwinia cancerogena showed the highest (0.866) and lowest (0.115) oxygen quotient (qo2) values, respectively, when exposed to mineral oil. staphylococcus warneri and enterobacter cloacae ... | 1999 | 10582377 |
| identification and environmental detection of rhodococcus species by 16s rdna-targeted pcr. | bacteria of the genus rhodococcus can degrade a wide range of organic pollutants and catalyse many useful biotransformations. there is a need for improved tests to identify rhodococcus species. pcr-based methods for species identification offer advantages in terms of speed and accuracy over traditional methods and can allow direct detection of microbes in environmental samples., pcr tests, using primers targeted at species-specific sequences in the 16s rrna gene, were successfully developed for ... | 1999 | 10583674 |
| regiospecific internal desaturation of aliphatic compounds by a mutant rhodococcus strain. | a mutant rhodococcus strain lacking the ability to utilize 1-chlorohexadecane was found to cis-desaturate aliphatic compounds, such as 1-chlorohexadecane, n-hexadecane, and heptadecanonitrile, yielding corresponding products with a double bond mainly at the ninth carbon from the terminal methyl groups. a new oxidative pathway involving the cis-desaturation step was suggested for alkane utilization by rhodococcus spp. | 1999 | 10584034 |
| analysis of the reaction mechanism and substrate specificity of haloalkane dehalogenases by sequential and structural comparisons. | haloalkane dehalogenases catalyse environmentally important dehalogenation reactions. these microbial enzymes represent objects of interest for protein engineering studies, attempting to improve their catalytic efficiency or broaden their substrate specificity towards environmental pollutants. this paper presents the results of a comparative study of haloalkane dehalogenases originating from different organisms. protein sequences and the models of tertiary structures of haloalkane dehalogenases ... | 1999 | 10585505 |
| nad(+)-dependent (s)-specific secondary alcohol dehydrogenase involved in stereoinversion of 3-pentyn-2-ol catalyzed by nocardia fusca aku 2123. | an nad(+)-dependent alcohol dehydrogenase was purified to homogeneity from nocardia fusca aku 2123. the enzyme catalyzed (s)-specific oxidation of 3-pentyn-2-ol (pyoh), i.e., part of the stereoinversion reaction for the production of (r)-pyoh, which is a valuable chiral building block for pharmaceuticals, from the racemate. the enzyme used a broad variety of secondary alcohols including alkyl alcohols, alkenyl alcohols, acetylenic alcohols, and aromatic alcohols as substrates. the oxidation was ... | 1999 | 10586501 |
| haloalkane dehalogenases: structure of a rhodococcus enzyme. | the hydrolytic haloalkane dehalogenases are promising bioremediation and biocatalytic agents. two general classes of dehalogenases have been reported from xanthobacter and rhodococcus. while these enzymes share 30% amino acid sequence identity, they have significantly different substrate specificities and halide-binding properties. we report the 1.5 a resolution crystal structure of the rhodococcus dehalogenase at ph 5.5, ph 7.0, and ph 5.5 in the presence of nai. the rhodococcus and xanthobacte ... | 1999 | 10587433 |
| mediastinitis due to gordona sputi after cabg. | genus gordona is included in mycolic acid containing bacteria. this genus infection is very rare and occurs classically in immuno-compromised patients. we report a patient who developed mediastinitis due to gordona sputi after coronary artery bypass grafting (cabg) using left internal mammary artery. immunocompromised factors were not noticed in this case but postoperative bleeding, the most important risk factor of mediastinitis, was found in his course. the treatment was antibiotic therapy, su ... | 1999 | 10597001 |
| nitrilase of rhodococcus rhodochrous j1. conversion into the active form by subunit association. | nitrilase-containing resting cells of rhodococcus rhodochrous j1 converted acrylonitrile and benzonitrile to the corresponding acids, but the purified nitrilase hydrolyzed only benzonitrile, and not acrylonitrile. the activity of the purified enzyme towards acrylonitrile was recovered by preincubation with 10 mm benzonitrile, but not by preincubation with aliphatic nitriles such as acrylonitrile. it was shown by light-scattering experiments, that preincubation with benzonitrile led to the assemb ... | 2000 | 10601860 |
| a pentacoordinated di-n-carboxamido-dithiolato-o-sulfinato-iron(iii) complex related to the metal site of nitrile hydratase. | postcoordination oxidation by dioxygen of one of the thiolate groups in a pentadentate n(2)s(3) ligand results in an iron(iii) complex with two n-carboxamido, two thiolato, and one o-sulfinato ligands (see the cameron representation). this novel mixed coordination is similar to that determined for the inactive form of the nitrile hydratase from rhodococcus sp. n-771, but differs by the o versus s binding of the sulfinato ligand. | 1999 | 10602230 |
| effect of prophylactic administration of hyperimmune plasma to prevent rhodococcus equi infection on foals from endemically affected farms. | the effect on foals of prophylactic administration of hyperimmune plasma to prevent r. equi infection was investigated on three farms at which r. equi infection was endemic. sixteen foals between 10 and 39 days of age were intravenously given 1-21 of hyperimmune plasma. elisa antibody titres against r. equi were significantly increased and maintained at high levels for over 30 days in most of the recipient foals. the prevalence of r. equi infection was 6.3% (1/16) in the foals that received the ... | 1999 | 10605374 |
| search for an antibody profile of rhodococcus equi infection in aids patients despite the diversity of isolates and patient immune dysfunction. | diversity of virulence-associated antigens of rhodococcus equi was detected among thirteen strains isolated from aids patients on two continents. one out of four brazilian isolates presented the virulence-associated antigen of 15- to 17-kda, and the other three isolates had the 20-kda virulence-associated antigen. in contrast, only three out of nine italian isolates were positive for virulence-associated antigens - two for the 15- to 17-kda antigen and one for the 20-kda antigen. in four other i ... | 1999 | 10611743 |
| light inhibits rifampicin inactivation and reduces rifampicin resistance due to a cloned mycobacterial adp-ribosylation gene. | rifampicin is a principal drug used to combat infections by mycobacteria and related organisms. most strains of mycobacterium are able to inactivate this antibiotic by ribosylation via an adp-ribosylated intermediate. we found that this inactivation was inhibited by light at levels similar to those prevailing in laboratory environments. rifampicin resistance arising from the cloned adp-ribosyl transferase was also greatly diminished at these light levels. the cloned rhodococcus equi monooxygenas ... | 2000 | 10612740 |
| characterization of is1676 from rhodococcus erythropolis sq1. | to develop a transposable element-based system for mutagenesis in rhodococcus, we used the sacb gene from bacillus subtilis to isolate a novel transposable element, is1676, from r. erythropolis sq1. this 1693 bp insertion sequence is bounded by imperfect (10 out of 13 bp) inverted repeats and it creates 4 bp direct repeats upon insertion. comparison of multiple insertion sites reveals a preference for the sequence 5'-(c/t)ta(a/g)-3' in the target site. is1676 contains a single, large (1446 bp) o ... | 1999 | 10616714 |
| an extractive membrane biofilm reactor for degradation of 1,3-dichloropropene in industrial waste water. | a bacterial biofilm, capable of mineralising a technical mixture of cis- and trans-1,3-dichloropropene (dcpe), was enriched on the biomedium side of an extractive membrane biofilm reactor (embr). the membrane separates the biomedium from the industrial waste water, in terms of ph, ionic strength and the concentration of toxic chemicals. the biofilm, attached to a silicone membrane, is able to mineralize dcpe after its diffusion through the membrane. five bacterial strains with degradation capabi ... | 1999 | 10616720 |
| analysis of the relative abundance of different types of bacteria capable of toluene degradation in a compost biofilter. | a microbial community of a compost biofilter treating toluene vapors was investigated using serum-bottle assays and mineral-agar plates. toluene was not consumed in the absence of oxygen. however, filter-bed extracts exposed to toluene vapor as the only carbon source produced distinct colony types (phenotypic groups) that were counted separately. strains from each group were isolated and checked for toluene-degradation activity in serum bottles. only 15% of colonies were true toluene degraders. ... | 1999 | 10616721 |
| rhodococcus equi nosocomial meningitis cured by levofloxacin and shunt removal. | | 2000 | 10619769 |
| cloning and characterization of a novel cis-naphthalene dihydrodiol dehydrogenase gene (narb) from rhodococcus sp. ncimb12038. | rhodococcus sp. ncimb112038 can utilize naphthalene as its sole carbon and energy source. the gene encoding cis-naphthalene dihydrodiol dehydrogenase (narb) of this strain has been cloned and sequenced. expression of ncimb12038 cis-naphthalene dihydrodiol dehydrogenase was demonstrated in escherichia coli cells. narb encodes a putative protein of 271 amino acids and shares 39% amino acid identity with the cis-naphthalene dihydrodiol dehydrogenase from pseudomonas putida g7. comparison of narb wi ... | 2000 | 10620687 |
| cobalt-substituted fe-type nitrile hydratase of rhodococcus sp. n-771. | when the genes encoding alpha and beta subunits of fe-type nitrile hydratase (nhase) from rhodococcus sp. n-771 were expressed in escherichia coli in co-supplemented medium without co-expression of the nhase activator, the nhase specifically incorporated not fe but co ion into the catalytic center. the produced co-substituted enzyme exhibited rather weak nhase activity, initially. however, the activity gradually increased by the incubation with an oxidizing agent, potassium hexacyanoferrate. the ... | 2000 | 10631329 |
| biochemical identification and biophysical characterization of a channel-forming protein from rhodococcus erythropolis. | organic solvent extracts of whole cells of the gram-positive bacterium rhodococcus erythropolis contain a channel-forming protein. it was identified by lipid bilayer experiments and purified to homogeneity by preparative sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis (page). the pure protein had a rather low molecular mass of about 8.4 kda, as judged by sds-page. sds-resistant oligomers with a molecular mass of 67 kda were also observed, suggesting that the channel is formed by ... | 2000 | 10633112 |
| humus bacteria of norway spruce stands: plant growth promoting properties and birch, red fescue and alder colonizing capacity. | we studied the potential of the humus layer of the norway spruce stands to supply beneficial rhizobacteria to birch (betula pendula), alder (alnus incana) and fescue grass (festuca rubra), representatives of pioneer vegetation after clear-cutting of the coniferous forest. axenically grown seedlings of these species were inoculated with the acid spruce humus, ph 3.7-5.3. actinorhizal propagules, capable of nodulating alder, were present in high density (10(3) g(-1)) in humus of long-term limed pl ... | 2000 | 10640667 |
| [pulmonary infection from rhodococcus equi after renal transplantation. review of the literature]. | rhodococcus equi, a strictly aerobic gram positive coco-bacillus, is a pathogen for horses and foals. it may induce opportunistic infections and is described in aids infected patients. we report the case of a 47-year old man, breeder of horses, with kidney transplant who has presented, 8 years after his graft, an impairment of health, a fever and evidence of pulmonary disease. the pulmonary biopsy under scanner guidance and microbiology study, has displayed the diagnosis of rhodococcus equi infe ... | 1999 | 10642987 |
| [bacteremic pneumonia due to rhodococcus equi in a patient with human immunodeficiency virus infection and visceral leishmaniasis]. | | 1999 | 10650653 |
| characterization of the 20s proteasome from the actinomycete frankia. | frankia is an actinomycete that fixes atmospheric nitrogen in symbiotic association with the root systems of a variety of non-leguminous plants, denominated actinorhizal plants. information on the biology of proteolysis in frankia is almost non-existent as it is extremely difficult to grow this organism. we have purified 20s proteasomes from frankia strain acn14a/ts-r. it is composed of one alpha-subunit and one beta-subunit, which assemble into the canonical structure of four rings of seven sub ... | 2000 | 10652097 |
| the rhodococcus fascians-plant interaction: morphological traits and biotechnological applications. | rhodococcus fascians is a gram-positive bacterium that infects dicotyledonous and monocotyledonous plants, leading to an alteration in the normal growth process of the host. the disease results from the modulation of the plant hormone balances, and cytokinins are thought to play an important role in the induction of symptoms. generally, on the aerial parts of the plants, existing meristems were found to be most sensitive to the action of r. fascians, but, depending on the infection procedure, di ... | 2000 | 10664130 |
| [conjugative transfer of a plasmid from escherichia coli to various strains of the order actinomycetales]. | the conjugal transfer of autonomous and integrative plasmids from the donor strain escherichia coli s17-1 to strains of genera actinomadura, arthrobacter, kitasatoa, micromonospora, nocardia, rhodococcus, saccharopolyspora, and to 16 strains of the genus streptomyces was demonstrated. the status of plasmids in recipient strains and the stability of their inheritance were analyzed. plasmids constructed for strains of the genus streptomyces were shown to function in a large number of strains belon ... | 1999 | 10687092 |
| gene cloning and nucleotide sequencing and properties of a cocaine esterase from rhodococcus sp. strain mb1. | a strain of rhodococcus designated mb1, which was capable of utilizing cocaine as a sole source of carbon and nitrogen for growth, was isolated from rhizosphere soil of the tropane alkaloid-producing plant erythroxylum coca. a cocaine esterase was found to initiate degradation of cocaine, which was hydrolyzed to ecgonine methyl ester and benzoate; both of these esterolytic products were further metabolized by rhodococcus sp. strain mb1. the structural gene encoding a cocaine esterase, designated ... | 2000 | 10698749 |
| evaluation of mini-vidas rapid test for detection of listeria monocytogenes from production lines of fresh to cold-smoked fish. | this study was conducted to evaluate the efficacy of the mini-vidas listeria monocytogenes (lmo) system (biomérieux vitek, inc., missouri, usa) for detection of l. monocytogenes in environmental and fish samples from three portuguese cold-smoking plants and from their fresh fish suppliers. mini-vidas-lmo is a fully automated system that uses fluorescent elfa (enzyme linked fluorescent assay) technology for detection of listeria monocytogenes antigens in food. it can be a rapid screening method a ... | 2000 | 10699670 |
| antimicrobial activity of o-carboranylalanine. | functionalized polyhedral carboranes, including amino acid analogs, have unique physicochemical properties and are used as experimental anticancer agents. however, our current knowledge on their effect in nonmammalian biological systems is limited. we investigated the activity spectrum in vitro of o-carboranylalanine (o-cba), considered to be a highly lipophilic analog of phenylalanine, against representative plant pathogenic bacteria and fungi of various taxonomic position. the antibacterial ef ... | 1999 | 10707765 |
| characterization of the gene cluster involved in isoprene metabolism in rhodococcus sp. strain ad45. | the genes involved in isoprene (2-methyl-1,3-butadiene) utilization in rhodococcus sp. strain ad45 were cloned and characterized. sequence analysis of an 8.5-kb dna fragment showed the presence of 10 genes of which 2 encoded enzymes which were previously found to be involved in isoprene degradation: a glutathione s-transferase with activity towards 1,2-epoxy-2-methyl-3-butene (isoi) and a 1-hydroxy-2-glutathionyl-2-methyl-3-butene dehydrogenase (isoh). furthermore, a gene encoding a second gluta ... | 2000 | 10715003 |
| trehalose 6,6'-dimycolate (cord factor) enhances neovascularization through vascular endothelial growth factor production by neutrophils and macrophages. | trehalose 6,6'-dimycolate (tdm) plays important roles in the development of granulomatous inflammation during infection with mycobacterium spp., rhodococcus spp., etc. to reveal the augmenting effect of tdm on vascular endothelial growth factor (vegf) production and neovascularization, we investigated murine granulomatous tissue air pouches induced by rhodococcus sp. strain 4306 tdm dissolved in freund's incomplete adjuvant (fia), comparing them to pouches treated with fia alone. histologically, ... | 2000 | 10722600 |
| cloning of an orf with homology to mycobacterium echa1, encoding the enoyl-coa hydratase, in rhodococcus fascians. | an open reading frame encoding a polypeptide of significant homology (55.7% identity) with the enoyl-coa hydratase encoded by the gene echa1 from mycobacterium tuberculosis has been found in the genome of the plant-pathogen bacteria rhodococcus fascians strain nrrl-b-15096. sequence alignments showed that it possesses several conserved blocks common to e. coli, m. tuberculosis and human mitochondria. one of such blocks includes a glutamate residue located at position 149, corresponding to the gl ... | 1999 | 10727085 |
| complement activation by mycoloyl glycolipids from mycobacterium tuberculosis and rhodococcus ruber. | in this study, we examined complement activation by mycoloyl glycolipids (mgl) such as trehalose-6,6'-dimycolate (tdm), often termed cord factor, and trehalose-6-monomycolate from mycobacterium tuberculosis and rhodococcus ruber, and also examined the effect of complement binding to mgl on phagocytosis by human monocytes. tdm and tmm, but not glucose mycolate, mannose mycolate or fructose mycolate which differ from tmm only in carbohydrate moiety, exhibited complement activation. tdm and tmm of ... | 1999 | 10730082 |
| operon structure and functional analysis of the genes encoding thermophilic desulfurizing enzymes of paenibacillus sp. a11-2. | paenibacillus a11-2 can efficiently cleave two carbon&bond;sulfur bonds in dibenzothiophene (dbt) and alkyl dbts, which are refractory by conventional petroleum hydrodesulfurization, to remove sulfur atom at high temperatures. an 8.7-kb dna fragment containing the genes for the dbt desulfurizing enzymes of a11-2 was cloned in escherichia coli and characterized. heterologous expression analysis of the deletion mutants identified three open reading frames that were required for the desulfurization ... | 2000 | 10733908 |
| isolation of rare opportunistic pathogens in hungary: case report and short review of the literature. rhodococcus equi. | rhodococcus equi is a well-established pathogen in foal pneumonia and is increasingly recognized as a pathogen in immunocompromised humans. we have isolated a gram-positive coccobacillus from 8 blood samples and lung tissues of a renal transplant patient. colony morphology, growth in lowenstein-jensen medium, 21 biochemical reactions, the characteristic morphological cycle (coccus-rod-coccus) and the camp test established the r. equi diagnosis. histological studies of 2 lung biopsy specimens rev ... | 2000 | 10735185 |
| roles of horizontal gene transfer and gene integration in evolution of 1,3-dichloropropene- and 1,2-dibromoethane-degradative pathways. | the haloalkane-degrading bacteria rhodococcus rhodochrous ncimb13064, pseudomonas pavonaceae 170, and mycobacterium sp. strain gp1 share a highly conserved haloalkane dehalogenase gene (dhaa). here, we describe the extent of the conserved dhaa segments in these three phylogenetically distinct bacteria and an analysis of their flanking sequences. the dhaa gene of the 1-chlorobutane-degrading strain ncimb13064 was found to reside within a 1-chlorobutane catabolic gene cluster, which also encodes a ... | 2000 | 10735862 |
| production of isopropyl cis-6-hexadecenoate by regiospecific desaturation of isopropyl palmitate by a double mutant of a rhodococcus strain. | resting cells of a double mutant noted as ksm-mt66, derived from rhodococcus sp. strain ksm-b-3 by uv irradiation, were found to cis-desaturate isopropyl hexadecanoate, yielding isopropyl cis-6-hexadecenoate. addition of sodium glutamate (1.0%), mg so4 (2 mm), and thiamine (2 mm) increased the productivity of the unsaturated product in phosphate buffer. optimal temperature and ph for the reaction were around 26 degrees c and 7, respectively. under the optimized conditions, more than 50 g/l of is ... | 2000 | 10737199 |
| mycolic acids from rhodococcus, gordonia, and dietzia. | the mycolic acids from 11 species of rhodococcus, seven species of gordonia, and one species of dietzia were analyzed using capillary gas chromatography and mass spectrometry (glc/ms). all strains tested in this study were divided into three groups according to the degree of double bonds and the average carbon number (av.nc.) of their mycolic acids. the genus gordonia belongs to the first group possessing an av.nc. in the upper 50s and 60s with 0 to 5 double bonds. some rhodococcus species posse ... | 2000 | 10739337 |
| characterization of the protocatechuic acid catabolic gene cluster from streptomyces sp. strain 2065. | protocatechuate 3,4-dioxygenase (ec 1.13.11.3) catalyzes the ring cleavage step in the catabolism of aromatic compounds through the protocatechuate branch of the beta-ketoadipate pathway. a protocatechuate 3,4-dioxygenase was purified from streptomyces sp. strain 2065 grown in p-hydroxybenzoate, and the n-terminal sequences of the beta- and alpha-subunits were obtained. pcr amplification was used for the cloning of the corresponding genes, and dna sequencing of the flanking regions showed that t ... | 2000 | 10742233 |
| nitrile hydratase and amidase from rhodococcus rhodochrous hydrolyze acrylic fibers and granular polyacrylonitriles. | rhodococcus rhodochrous ncimb 11216 produced nitrile hydratase (320 nkat mg of protein(-1)) and amidase activity (38.4 nkat mg of protein(-1)) when grown on a medium containing propionitrile. these enzymes were able to hydrolyze nitrile groups of both granular polyacrylonitriles (pan) and acrylic fibers. nitrile groups of pan40 (molecular mass, 40 kda) and pan190 (molecular mass, 190 kda) were converted into the corresponding carbonic acids to 1.8 and 1.0%, respectively. in contrast, surfacial n ... | 2000 | 10742253 |
| successful treatment of rhodococcus equi pulmonary infection in a renal transplant recipient. | the rhodococcus is a mycobacterium-like organism which is normally a pathogen in foals. it usually spreads by direct contact or by aerosol from horse faeces and causes pyogranulomatous pulmonary infections. occasionally, it acts opportunistically to infect immuno-compromised human hosts, most commonly those with the acquired immune deficiency syndrome (aids). here we report a pulmonary infection by rhodococcus equi in a renal transplant recipient who was successfully treated. the literature on t ... | 2000 | 10743428 |
| [adaptation of acrylamide producer rhodococcus rhodochrous m8 to change in ammonium concentration in medium]. | the mechanism of adaptation of the acrylamide producing strain rhodococcus rhodochrous m8 to changes in ammonium concentrations in the medium was studied. an increase in the content of ammonium in the medium changed the activity of glutamine synthetase (gs) (ec 6.3.1.2) and glutamine dehydrogenase (gd) (ec 1.4.1.4), the enzymes of ammonium assimilation, as well as the activities of enzymes responsible for nitrile utilization: nitrile hydratase (ec 4.2.1.84) and amidase (ec 3.5.1.4). this also ca ... | 2000 | 10752079 |
| [storage of industrial microorganisms entrapped into polymer matrices]. | our study of the techniques of long-term storage of the biomass of various strains of microorganisms, which cause breakdown or transformation of synthetic organic compounds, demonstrates that desiccated agar beads with immobilized microbial cells can be used for this purpose. in addition, the cells can be stored in desiccated matrices of agar or polyvinyl alcohol, coating synthetic cords. such dry biocatalysts may be used for quick starting of bioreactors and in other biotechnological processes. ... | 2000 | 10752086 |
| characterization of the naphthalene-degrading bacterium, rhodococcus opacus m213. | bacterial strain m213 was isolated from a fuel oil-contaminated soil in idaho, usa, by growth on naphthalene as a sole source of carbon, and was identified as rhodococcus opacus m213 by 16s rdna sequence analysis and growth on substrates characteristic of this species. m213 was screened for growth on a variety of aromatic hydrocarbons, and growth was observed only on simple 1 and 2 ring compounds. no growth or poor growth was observed with chlorinated aromatic compounds such as 2,4-dichloropheno ... | 2000 | 10754253 |
| heterologous expression of bacterial epoxyalkane:coenzyme m transferase and inducible coenzyme m biosynthesis in xanthobacter strain py2 and rhodococcus rhodochrous b276. | coenzyme m (com) (2-mercaptoethanesulfonic acid) biosynthesis is shown to be coordinately regulated with the expression of the enzymes of alkene and epoxide metabolism in the propylene-oxidizing bacteria xanthobacter strain py2 and rhodococcus rhodochrous strain b276. these results provide the first evidence for the involvement of com in propylene metabolism by r. rhodochrous and demonstrate for the first time the inducible nature of eubacterial com biosynthesis. | 2000 | 10762269 |
| synthesis and antifungal activity of rhodopeptin analogues. 1. modification of the east and south amino acid moieties. | structure-activity relationships of the east and south amino acid modified analogues of rhodopeptins, novel antifungal cyclic tetrapeptides isolated from rhodococcus species mer-n1033, have been investigated. it was observed that a basic amino acid moiety (lysine or ornithine) as the east amino acid and a hydrophobic and bulky neutral amino acid (i.e., gamma-methylleucine) as the south amino acid were indispensable structure motifs for antifungal activity of rhodopeptin analogues. | 2000 | 10768200 |
| synthesis and antifungal activity of rhodopeptin analogues. 2. modification of the west amino acid moiety. | structure-activity relationships of the west amino acid modified analogues of rhodopeptins, novel antifungal tetrapeptide isolated from rhodococcus species mer-n1033, have been investigated. among the analogues synthesized, 2,2-difluoro and 2-hydroxy derivatives retained the antifungal activity with better physical properties, i.e., solubility or acute toxicity. | 2000 | 10768201 |
| purification and characterization of a baeyer-villiger mono-oxygenase from rhodococcus erythropolis dcl14 involved in three different monocyclic monoterpene degradation pathways. | a baeyer-villiger mono-oxygenase (bvmo), catalysing the nadph- and oxygen-dependent oxidation of the monocyclic monoterpene ketones 1-hydroxy-2-oxolimonene, dihydrocarvone and menthone, was purified to homogeneity from rhodococcus erythropolis dcl14. monocyclic monoterpene ketone mono-oxygenase (mmkmo) is a monomeric enzyme of molecular mass 60 kda. it contains 1 mol of fad/monomer as the prosthetic group. the n-terminal amino acid sequence showed homology with many other nadph-dependent and fad ... | 2000 | 10769172 |
| pulmonary infection in human immunodeficiency virus-infected patients in taiwan. | pulmonary infection is a major cause of morbidity and mortality in human immunodeficiency virus (hiv)-infected patients. | 2000 | 10770026 |
| mics of oxazolidinones for rhodococcus equi strains isolated from humans and animals. | eperezolid and linezolid are representatives of a new class of orally active, synthetic antimicrobial agents. the in vitro activity values (mics) of linezolid, eperezolid, and comparator antibiotics against 102 strains of rhodococcus equi isolated from humans and animals were determined. linezolid was more active than eperezolid against the strains tested; premafloxacin was the most active comparator antibiotic. | 2000 | 10770781 |
| [the degree of halophily in rhodococcus erythropolis and halobacterium salinarum depends on the partial pressure of oxygen]. | | 2000 | 10776634 |
| effects of non-ionic surfactants on the uptake and hydrolysis of fluoresceindiacetate by alkane-oxidizing bacteria. | biological effects of non-ionic surfactants on alkane-oxidizing bacteria were studied by assessing their influence on the uptake of prefluorochrome fluoresceindiacetate (fda) and its intracellular hydrolysis to fluorescein. both decreasing and increasing rates of hydrolysis as a consequence of the presence of surfactants were observed. the surfactants influenced the uptake of fda, but not its intracellular hydrolysis. the effects of the surfactants on the uptake rate depended strongly on the str ... | 2000 | 10779877 |
| haloalkane-utilizing rhodococcus strains isolated from geographically distinct locations possess a highly conserved gene cluster encoding haloalkane catabolism. | the sequences of the 16s rrna and haloalkane dehalogenase (dhaa) genes of five gram-positive haloalkane-utilizing bacteria isolated from contaminated sites in europe, japan, and the united states and of the archetypal haloalkane-degrading bacterium rhodococcus sp. strain ncimb13064 were compared. the 16s rrna gene sequences showed less than 1% sequence divergence, and all haloalkane degraders clearly belonged to the genus rhodococcus. all strains shared a completely conserved dhaa gene, suggesti ... | 2000 | 10781539 |
| nicotinoprotein (nadh-containing) alcohol dehydrogenase from rhodococcus erythropolis dsm 1069: an efficient catalyst for coenzyme-independent oxidation of a broad spectrum of alcohols and the interconversion of alcohols and aldehydes. | extracts from benzyl-alcohol-grown rhodococcus erythropolis dsm 1069 showed nad(p)-independent, n,n-dimethyl-4-nitrosoaniline (ndma)-dependent alcohol dehydrogenase activity. the enzyme exhibiting this activity was purified to homogeneity and characterized. it appears to be a typical nicotinoprotein as it contains tightly bound nadh acting as cofactor instead of coenzyme. other characteristics indicate that it is highly similar to the known nicotinoprotein alcohol dehydrogenase (np-adh) from amy ... | 2000 | 10784035 |
| targeted disruption of the kstd gene encoding a 3-ketosteroid delta(1)-dehydrogenase isoenzyme of rhodococcus erythropolis strain sq1. | microbial phytosterol degradation is accompanied by the formation of steroid pathway intermediates, which are potential precursors in the synthesis of bioactive steroids. degradation of these steroid intermediates is initiated by delta(1)-dehydrogenation of the steroid ring structure. characterization of a 2.9-kb dna fragment of rhodococcus erythropolis sq1 revealed an open reading frame (kstd) showing similarity with known 3-ketosteroid delta(1)-dehydrogenase genes. heterologous expression of k ... | 2000 | 10788377 |
| identification of fluoropyrogallols as new intermediates in biotransformation of monofluorophenols in rhodococcus opacus 1cp. | the transformation of monofluorophenols by whole cells of rhodococcus opacus 1cp was investigated, with special emphasis on the nature of hydroxylated intermediates formed. thin-layer chromatography, mass spectrum analysis, and (19)f nuclear magnetic resonance demonstrated the formation of fluorocatechol and trihydroxyfluorobenzene derivatives from each of three monofluorophenols. the (19)f chemical shifts and proton-coupled splitting patterns of the fluorine resonances of the trihydroxyfluorobe ... | 2000 | 10788394 |
| resistance of rhodococcus equi to acid ph. | rhodococcus equi is an important gram-positive intracellular facultative pathogen in foals of less than 3 months of age, that causes suppurative bronchopneumonia, lymphadenitis and/or enteritis. the disease in young foals mainly occurs in spring and summer when weather conditions are favorable for survival and multiplication of the bacteria in the environment. r. equi is widespread in the environment of horsebreeding farms: it has been isolated from the soil of paddocks and from the feces of adu ... | 2000 | 10791761 |
| evidence for diverse oxidations in the catabolism of toluene by rhodococcus rhodochrous strain ofs. | rhodococcus rhodochrous strain ofs grew on toluene as a sole source of carbon and energy with a maximum growth rate of 0.011 h(-1). initial reaction products were extracted, derivatized and identified by gc-ms. oxygen consumption studies indicated that ofs grown on an aliphatic substrate required an induction period before oxidizing toluene. ofs grown on toluene transformed an array of aromatic ground water pollutants including styrene, ethylbenzene and chlorobenzene. products of these transform ... | 2000 | 10803902 |
| alkylated benzothiophene desulfurization by rhodococcus sp. strain t09. | a benzothiophene desulfurizing bacterium was isolated and identified as rhodococcus sp. strain t09. growth assays revealed that this strain assimilated, as the sole sulfur source, various organosulfur compounds that cannot be assimilated by the well-studied dibenzothiophene-desulfurizing rhodococcus sp. igts8. the cellular growth rate of strain t09 for the alkylated benzothiophenes depended on the alkylated position and the length of the alkyl moiety. | 2000 | 10803960 |
| [dependence of transformation of chlorophenols by rhodococci on position and number of chlorine atoms in the aromatic ring]. | study of the conversion of chlorophenols by rhodococcus opacus 1g, r. rhodnii 135, r. rhodochrous 89, and r. opacus 1cp disclosed the dependence of the conversion rate and pathway on the number and position of chlorine atoms in the aromatic ring. the most active chlorophenol converter, strain r. opacus 1cp, grew on each of the three isomeric monochlorophenols and on 2,4-dichlorophenol; the rate of growth decreased from 4-chlorophenol to 3-chlorophenol and then to 2-chlorophenol. the parameters o ... | 2000 | 10808489 |
| [effect of composition of cellular lipids of formation of nonspecific antibiotic resistance of alkanotrophic rhodocoocci]. | the antibiotic resistance and lipid composition of rhodococci grown in rich organic media with gaseous or liquid n-alkanes were studied. hydrocarbon-grown rhodococci exhibited an increased resistance to a wide range of antibiotics (aminoglycosides, linkosamides, macrolides, beta-lactams, and aromatic compounds). the enhanced antibiotic resistance of rhodococci grown on n-alkanes correlated with an increased content of total cell lipids (up to 14-28%) and saturated straight-chain fatty acids (c16 ... | 2000 | 10808491 |
| cooperation between reactive oxygen and nitrogen intermediates in killing of rhodococcus equi by activated macrophages. | rhodococcus equi is a facultative intracellular bacterium of macrophages which can infect immunocompromised humans and young horses. in the present study, we examine the mechanism of host defense against r. equi by using a murine model. we show that bacterial killing is dependent upon the presence of gamma interferon (ifn-gamma), which activates macrophages to produce reactive nitrogen and oxygen intermediates. these two radicals combine to form peroxynitrite (onoo(-)), which kills r. equi. mice ... | 2000 | 10816516 |
| efficient production of desulfurizing cells with the aid of expert system. | an expert system was used to achieve the high production of desulfurizing cells of rhodococcus erythropolis ka 2-5-1. by adding a proper amount of sulfur containing component with the aid of the expert system, we could avoid excess feeding which resulted in the lowering of desulfurizing activity and starvation which caused serious damage to cell growth. in order to determine the addition amount by the expert system, the data of the amount of chemical elements contained in the cells were used as ... | 2000 | 10817820 |
| cholesterol oxidase: sources, physical properties and analytical applications. | since flegg (h.m. flegg, an investigation of the determination of serum cholesterol by an enzymatic method, ann. clin. biochem. 10 (1973) 79-84) and richmond (w. richmond, the development of an enzymatic technique for the assay of cholesterol in biological fluids, scand. j. clin. lab. invest. 29 (1972) 25; w. richmond, preparation and properties of a bacterial cholesterol oxidase from nocardia sp. and its application to enzyme assay of total cholesterol in serum, clinical chemistry 19 (1973) 135 ... | 2000 | 10822008 |
| methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates. | miniaturized growth systems for heterogeneous culture collections are not only attractive in reducing demands for incubation space and medium but also in making the parallel handling of large numbers of strains more practicable. we report here on the optimization of oxygen transfer rates in deep-well microtiter plates and the development of a replication system allowing the simultaneous and reproducible sampling of 96 frozen glycerol stock cultures while the remaining culture volume remains froz ... | 2000 | 10831450 |
| metabolism of carveol and dihydrocarveol in rhodococcus erythropolis dcl14. | rhodococcus erythropolis dcl14 assimilates all stereoisomers of carveol and dihydrocarveol as sole source of carbon and energy. induction experiments with carveol- or dihydrocarveol-grown cells showed high oxygen consumption rates with these two compounds and with carvone and dihydrocarvone. (dihydro)carveol-grown cells of r. erythropolis dcl14 contained the following enzymic activities involved in the carveol and dihydrocarveol degradation pathways of this micro-organism: (dihydro)carveol dehyd ... | 2000 | 10832640 |
| rhodococcus opacus strain pd630 as a new source of high-value single-cell oil? isolation and characterization of triacylglycerols and other storage lipids. | the triacylglycerol (tag)-accumulating, hydrocarbon-degrading bacterium rhodococcus opacus strain pd630 and chemically induced storage-deficient mutants derived from this strain were investigated for their capability to accumulate storage lipids in the cytoplasm during cultivation under nitrogen-limiting conditions. acylglycerols were analysed by matrix-associated laser desorption ionization-time of flight mass spectrometry (maldi-tof) and by reversed-phase hplc. fatty acids comprising 13-19 car ... | 2000 | 10832641 |
| detection of the 20-kda virulence-associated antigen of rhodococcus equi in malakoplakia-like lesion in pleural tissue obtained from an aids patient. | a malakoplakia-like lesion was detected in a pleural biopsy from an aids patient presenting clinical and radiologic features of pneumonia. cultures of bronchoalveolar lavage and pleural fluid evidenced rhodococcus equi as the causative agent of pleuro-pulmonary infection. immunochemical characterization of the r. equi isolate showed the presence of a strain similar to the atcc 33704 reference strain presenting the capsular antigen of serotype 4, and the intermediate virulence-associated antigen ... | 2000 | 10834389 |
| rhodococcus koreensis sp. nov., a 2,4-dinitrophenol-degrading bacterium. | a 2,4-dinitrophenol-degrading bacterial strain, dnp505t, which was isolated from an industrial wastewater, was taxonomically studied by a polyphasic approach using phenotypic, chemotaxonomic and genetic methods. strain dnp505t has a cell wall of chemotype iv containing meso-diaminopimelic acid, arabinose and galactose. the predominant menaquinone is mk-8(h2). mycolic acids contain 43-53 carbon atoms. strain dnp505t has a cellular fatty acid profile containing straight-chain saturated, unsaturate ... | 2000 | 10843063 |
| [molecular biology of enzymes degrading pollutants: diversity and convergent evolution observed on extradiol dioxygenases]. | | 2000 | 10846472 |
| post-translational modification is essential for catalytic activity of nitrile hydratase. | nitrile hydratase from rhodococcus sp. n-771 is an alphabeta heterodimer with a nonheme ferric iron in the catalytic center. in the catalytic center, alphacys112 and alphacys114 are modified to a cysteine sulfinic acid (cys-so2h) and a cysteine sulfenic acid (cys-soh), respectively. to understand the function and the biogenic mechanism of these modified residues, we reconstituted the nitrile hydratase from recombinant unmodified subunits. the alphabeta complex reconstituted under argon exhibited ... | 2000 | 10850812 |
| desulfurization of alkylated forms of both dibenzothiophene and benzothiophene by a single bacterial strain. | thirty-five bacterial strains capable of converting dibenzothiophene into 2-hydroxybiphenyl were isolated. among them rhodococcus erythropolis ka2-5-1 was chosen for further characterization because of its ability to retain high desulfurization activity stably. pcr cloning and dna sequencing of a ka2-5-1 genomic dna fragment showed that it was practically identical with dszabc genes from rhodococcus sp. igts8, a representative carbon-sulfur-bond-targeted dibenzothiophene-degrading bacterium. ka2 ... | 2000 | 10856644 |
| real-time monitoring of nitrile biotransformations by mid-infrared spectroscopy. | in this study mid-infrared spectroscopy was used to follow the enzyme kinetics involved in nitrile biocatalysis using whole cell suspensions of the bacterium rhodococcus rhodochrous ll100-21. the bacteria were grown on acetonitrile to induce a two-step enzymatic pathway. acetonitrile was biotransformed to acetamide by a nitrile hydratase enzyme and subsequently to acetic acid (carboxylate ion) by an amidase enzyme. the bacteria were also grown on benzonitrile to induce a one-step enzymatic pathw ... | 2000 | 10856779 |
| room-temperature ionic liquids as replacements for organic solvents in multiphase bioprocess operations. | organic solvents are widely used in a range of multiphase bioprocess operations including the liquid-liquid extraction of antibiotics and two-phase biotransformation reactions. there are, however, considerable problems associated with the safe handling of these solvents which relate to their toxic and flammable nature. in this work we have shown for the first time that room-temperature ionic liquids, such as 1-butyl-3-methylimi- dazolium hexafluorophosphate, [bmim][pf(6)], can be successfully us ... | 2000 | 10861402 |
| enzymatic dehalogenation of gas phase substrates with haloalkane dehalogenase. | haloalkane dehalogenase is an enzyme capable of catalyzing the conversion of short-chained (c(2)-c(8)) aliphatic halogenated hydrocarbons to a corresponding primary alcohol. because of its broad substrate specificity for mono-, di-, and trisubstituted halogenated hydrocarbons and cofactor independence, haloalkane dehalogenases are attractive biocatalysts for gas-phase bioremediation of pollutant halogenated vapor emissions. a solid preparation of haloalkane dehalogenase from rhodococcus rhodochr ... | 2000 | 10861403 |
| change in natural history of opportunistic infections in hiv-infected patients. | | 2000 | 10862994 |
| hiv-infected patient with a rhodococcus equi pneumonia. | the case history of a hiv patient with a pulmonary infect of rhodococcus equi is presented. he recovered after prolonged treatment with antibiotics and lobectomy. the rhodococcus equi infection was the presenting symptom of his impaired immune status caused by hiv infection. | 2000 | 10862999 |
| [oxidation of organic compounds by rhodococcus erythropolis 3/89 propanomonooxygenase]. | the ability of propane monooxygenase of rhodococcus erythropolis 3/89 to catalyze oxidation of higher liquid alkenes and aromatic hydrocarbons was studied. optimal conditions of tetradecene epoxidation and benzene hydroxylation were found. under these conditions, oxidation was shown to be accompanied by a 100% conversion of benzene to phenol. | 2000 | 10867943 |
| degradation of anaerobic reductive dechlorination products of aroclor 1242 by four aerobic bacteria. | we studied the aerobic degradation of eight pcb congeners which comprise from 70 to 85% of the anaerobic dechlorination products from aroclor 1242, including 2-, 4-, 2,4-, 2,6-, 2,2'-, 2,4'-, 2,2', 4-, and 2,4,4'-chlorobiphenyl (cb), and the biodegradation of their mixtures designed to simulate anaerobic dechlorination profiles m and c. strains comamonas testosteroni vp44 and rhodococcus erythreus ny05 preferentially oxidized a para-substituted ring, while rhodococcus sp. rha1, similar to well k ... | 1999 | 10870552 |
| cloning and expression of gene encoding a novel endoglycoceramidase of rhodococcus sp. strain c9. | endoglycoceramidase (egcase) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. we previously reported that the asn-glu-pro (nep) sequence is part of the active site of egcase of rhodococcus sp. strain m-777. this paper describes the molecular cloning of a new egcase gene utilizing the nep sequence from the genomic library of rhodococcus sp. strain c9, which was clearly distinguishable from m-777 by 16s rdna analysis. c9 ... | 2000 | 10876169 |
| molecular analysis of surfactant-driven microbial population shifts in hydrocarbon-contaminated soil. | we analyzed the impact of surfactant addition on hydrocarbon mineralization kinetics and the associated population shifts of hydrocarbon-degrading microorganisms in soil. a mixture of radiolabeled hexadecane and phenanthrene was added to batch soil vessels. witconol sn70 (a nonionic, alcohol ethoxylate) was added in concentrations that bracketed the critical micelle concentration (cmc) in soil (cmc') (determined to be 13 mg g(-1)). addition of the surfactant at a concentration below the cmc' (2 ... | 2000 | 10877792 |
| substrate specificity of regiospecific desaturation of aliphatic compounds by a mutant rhodococcus strain. | substrate specificity of cis-desaturation of alipahtic compounds by resting cells of a mutant, rhodococcus sp. strain ksm-mt66, was examined. among substrates tested, the rhodococcal cells were able to convert n-alkanes (c13-c19), 1-chloroalkanes (c16 and c18), ethyl fatty acids (c14-c17) and alkyl (c1-c4) esters of palmitic acid to their corresponding unsaturated products of cis configuration. the products from n-alkanes and 1-chloroalkanes had a double bond mainly at the 9th carbon from their ... | 2000 | 10879483 |
| purification, characterization, and cdna cloning of a novel acidic endoglycoceramidase from the jellyfish, cyanea nozakii. | endoglycoceramidase (ec ) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides in various glycosphingolipids. we report here the purification, characterization, and cdna cloning of a novel endoglycoceramidase from the jellyfish, cyanea nozakii. the purified enzyme showed a single protein band estimated to be 51 kda on sds-polyacrylamide gel electrophoresis. the enzyme showed a ph optimum of 3.0 and was activated by triton x-100 and lubrol px but not by s ... | 2000 | 10882727 |
| a repeat-batch membrane bioreactor with a phase inversion for the desaturation of isopropyl palmitate by a mutant rhodococcus strain. | a repeat-batch membrane bioreactor was constructed for the novel bioconversion of isopropyl hexadecanoate to isopropyl cis-6-hexadecenoate by a rhodococcus mutant. the addition of glutamate, thiamine, and mgso(4) was very effective in improving not only the rate and yield of the bioconversion but also the maintenance of desaturation activity during cell recycling. an oil-in-water (o/w) type emulsion of the reaction medium was inverted to a water-in-oil (w/o) type by discharging the water phase f ... | 2000 | 10908790 |
| association of disease with isolation and virulence of rhodococcus equi from farm soil and foals with pneumonia. | to determine whether isolation and virulence of rhodococcus equi from soil and infected foals are associated with clinical disease. | 2000 | 10909463 |
| characterization of s-triazine herbicide metabolism by a nocardioides sp. isolated from agricultural soils. | atrazine, a herbicide widely used in corn production, is a frequently detected groundwater contaminant. nine gram-positive bacterial strains able to use this herbicide as a sole source of nitrogen were isolated from four farms in central canada. the strains were divided into two groups based on repetitive extragenic palindromic (rep)-pcr genomic fingerprinting with eric and boxa1r primers. based on 16s ribosomal dna sequence analysis, both groups were identified as nocardioides sp. strains. none ... | 2000 | 10919761 |
| rhodococcus l-phenylalanine dehydrogenase: kinetics, mechanism, and structural basis for catalytic specificity. | phenylalanine dehydrogenase catalyzes the reversible, pyridine nucleotide-dependent oxidative deamination of l-phenylalanine to form phenylpyruvate and ammonia. we have characterized the steady-state kinetic behavior of the enzyme from rhodococcus sp. m4 and determined the x-ray crystal structures of the recombinant enzyme in the complexes, e.nadh.l-phenylalanine and e.nad(+). l-3-phenyllactate, to 1.25 and 1.4 a resolution, respectively. initial velocity, product inhibition, and dead-end inhibi ... | 2000 | 10924111 |
| multiple respiratory bursts as a response to veratrate stress in rhodococcus erythropolis cells. | although rhodococcus spp. strains are able to degrade methoxyphenols by enzymatic means, the contact with veratric acid (3, 4-dimethoxybenzoic acid, hereafter called veratrate) is very stressful for the cells of rhodococcus erythropolis dsm 1069 (rh). within 5 min of contact veratrate in phosphate buffer, the emergence of many vacuoles was observed in the cell body and respiratory bursts, with violent endogenous oxygen uptake, took place several times during the 24 h incubation. during these pea ... | 2000 | 10924225 |
| pathogenicity of rhodococcus equi expressing a virulence-associated 20 kda protein (vapb) in foals. | rhodococcus equi strains of intermediate virulence (imv) for mice possess a 20kda protein designated virulence associated protein b (vapb) and a virulence plasmid of 79-100kb, and can be recovered from the submaxillary lymph nodes of pigs. the pathogenicity of such r. equi strains for foals is unknown. in this study, two foals, 42 and 43 days of age, were infected intratracheally with 10(6) and 10(9) cells of r. equi imv strain a5, respectively. the foal infected with 10(9) cells of strain a5 be ... | 2000 | 10925043 |
| propionicin sm1, a bacteriocin from propionibacterium jensenii df1: isolation and characterization of the protein and its gene. | we purified a bacteriocin from the cell-free supernatant of propionibacterium jensenii df1 isolated from swiss raw milk, and named it propionicin sm1. the heat-stable protein was strongly bactericidal against p. jensenii dsm20274. on the basis of the n-terminal amino acid sequence of the purified protein, a degenerate oligonucleotide probe was designed to locate and clone the corresponding gene of p. jensenii df1. it hybridized exclusively with the df1l-resident plasmid plme106, but not with chr ... | 2000 | 10930068 |
| lung nodular lesions in heart transplant recipients. | to describe the characteristics and etiology of lung nodules after heart transplantation (ht). during a 6-year period 147 patients received ht and 130 survived more than 1 week. nodular lesions were demonstrated after ht in 13 patients (10%). median age was 53 years, and all patients were male. nodules were detected 23 to 158 days after ht (median, 66 days). an etiologic diagnosis was made in all but 1 case: aspergillus (5), nocardia-rhodococcus (4), and cytomegalovirus (cmv) (3). previous sever ... | 2000 | 10930815 |
| microbial conversion of indene to indandiol: a key intermediate in the synthesis of crixivan. | indene is oxidized to mixtures of cis- and trans-indandiols and related metabolites by pseudomonas putida and rhodococcus sp. isolates. indene metabolism is consistent with monooxygenase and dioxygenase activity. p. putida resolves enantiomeric mixtures of cis-1,2-indandiol by further selective oxidation of the 1r, 2s-enantiomer yielding high enantiomeric purity of cis-(1s, 2r)-indandiol, a potential intermediate in the synthesis of indinavir sulfate (crixivan), a protease inhibitor used in the ... | 1999 | 10935755 |
| pharmacokinetics of erythromycin estolate and erythromycin phosphate after intragastric administration to healthy foals. | to determine pharmacokinetics and plasma concentrations of erythromycin and related compounds after intragastric administration of erythromycin phosphate and erythromycin estolate to healthy foals. | 2000 | 10951982 |
| [equine rhodococcus equi pneumonia: first report in israel and its significance for man]. | rhodococcus equi is an important pathogen in young horses. in recent years it has been increasingly recognized as an opportunistic infectious agent in patients with immune deficiency. the increase in recognized cases may be related to the increased prevalence of aids. however, more cases may have been recognized lately due to increased awareness of the pathogenicity of this bacterium. based on medical reports, there appears to be an association between rhodococcus equi infections and exposure to ... | 1999 | 10959322 |
| rapid characterisation of deep-sea actinomycetes for biotechnology screening programmes. | a continual need in natural product discovery is dereplication, that is the ability to exclude previously tested microorganisms from screening programmes. whole-cell fingerprinting techniques offer an ideal solution to this problem because of their rapidity and reproducibility, dependence on small samples, and automation. one such technique, curie-point pyrolysis mass spectrometry (pyms), has been deployed for the characterisation of a unique collection of actinomycetes recovered from pacific oc ... | 2000 | 10959565 |
| microbiology of the stalactites from grotta dei cervi, porto badisco, italy. | the active stalactites from grotta dei cervi, porto badisco, southeastern italy, were sampled to investigate the microbial communities present in these speleothems. sampling was carried out in a transect about 150 m long in the central gallery, where numerous gram-positive bacteria were isolated. actinomycetes of the genus streptomyces were the most abundant, followed by members of the genus bacillus. further isolates were assigned to the genera amycolatopsis, arthrobacter; agromyces. micrococcu ... | 2000 | 10963330 |