| comparative analysis of acid resistance in listeria monocytogenes and salmonella enterica strains before and after exposure to poultry decontaminants. role of the glutamate decarboxylase (gad) system. | data on the ability of chemical poultry decontaminants to induce an acid stress response in pathogenic bacteria are lacking. this study was undertaken in order to compare the survival rates in acid broths of listeria monocytogenes and salmonella enterica strains, both exposed to and not exposed to decontaminants. the contribution of the glutamate decarboxylase (gad) acid resistance system to the survival of bacteria in acid media was also examined. four strains (l. monocytogenes serovar 1/2, l. ... | 2009 | 19835779 |
| occurrence of foodborne pathogens in irish farmhouse cheese. | food safety is a critical factor in the production of farmhouse cheese. in ireland the varieties of farmhouse cheese produced reflect a much broader range than those produced commercially and some of these cheese varieties are associated with greater microbiological risk. these include cheese produced from unpasteurised milk and soft ripened cheese such as mould or smear-ripened cheeses which have high ph and relatively short ripening times. the aim of this study was to determine the microbiolog ... | 2009 | 19835780 |
| insertional mutagenesis of listeria monocytogenes 568 reveals genes that contribute to enhanced thermotolerance. | the objectives of this study were to identify molecular mechanisms of thermotolerance using transposon mutants of listeria monocytogenes 568, serotype 1/2a, and to compare their thermal death kinetics at 52, 56 and 60 degrees c. sixteen tn917 transposon mutants with enhanced heat resistance were acquired from a library of 4300 mutants following a multi-step screening process. genetic regions with tn917 insertions encompassed a broad range of functionalities including; transport, metabolism, repl ... | 2009 | 19836093 |
| diamide triggers mainly s thiolations in the cytoplasmic proteomes of bacillus subtilis and staphylococcus aureus. | glutathione constitutes a key player in the thiol redox buffer in many organisms. however, the gram-positive bacteria bacillus subtilis and staphylococcus aureus lack this low-molecular-weight thiol. recently, we identified s-cysteinylated proteins in b. subtilis after treatment of cells with the disulfide-generating electrophile diamide. s cysteinylation is thought to protect protein thiols against irreversible oxidation to sulfinic and sulfonic acids. here we show that s thiolation occurs also ... | 2009 | 19837798 |
| genetic features of resident biofilms determine attachment of listeria monocytogenes. | planktonic listeria monocytogenes cells in food-processing environments tend most frequently to adhere to solid surfaces. under these conditions, they are likely to encounter resident biofilms rather than a raw solid surface. although metabolic interactions between l. monocytogenes and resident microflora have been widely studied, little is known about the biofilm properties that influence the initial fixation of l. monocytogenes to the biofilm interface. to study these properties, we created a ... | 2009 | 19837841 |
| microbial induction of inflammatory bowel disease associated gene tl1a (tnfsf15) in antigen presenting cells. | tl1a is a member of the tnf superfamily and its expression is increased in the mucosa of inflammatory bowel disease patients. neutralizing anti-mouse tl1a ab attenuates chronic colitis in two t-cell driven murine models, suggesting that tl1a is a central modulator of gut mucosal inflammation in inflammatory bowel disease. we showed previously that tl1a is induced by immune complexes via the fc gamma r signaling pathway. in this study, we report that multiple bacteria, including gram negative org ... | 2009 | 19839006 |
| [listeriosis, a rare but severe foodborne infection]. | listeria monocytogenes is a gram-positive bacillus widespread in nature and responsible for human and animal infections. listeriosis is a severe and rare infection transmitted orally and vertically, frequently in the context of an underlying immunosuppression. l. monocytogenes is able to cross several barriers: intestinal mucosa, placenta and blood-brain barrier. listeriosis is associated with a range of clinical presentations: ranging from acute gastroenteritis, septicaemia, central nervous sys ... | 2009 | 19839454 |
| microbiological quality and safety of raw milk and soft cheese and detection of autochthonous lactic acid bacteria with antagonistic activity against listeria monocytogenes, salmonella spp., and staphylococcus aureus. | this study aimed to characterize the microbiological quality and safety of raw milk and soft cheese, verifying possible associations between microbial populations, and the detection of lactic acid bacteria (lab) with antagonistic activity against foodborne pathogens. raw milk (n = 36) and soft cheese (n = 18) samples were collected and submitted for the analysis of mesophilic aerobes, total coliforms, escherichia coli, lab, coagulase-positive staphylococcus (cps), listeria monocytogenes, and sal ... | 2010 | 19839761 |
| in vivo activities of recombinant divercin v41 and its structural variants against listeria monocytogenes. | recombinant divercin rv41 (dvnrv41) and its structural variants were used in this study to assess their antilisterial activities in vivo in mice challenged intravenously with listeria monocytogenes egde. treatment with dvnrv41 before and after infection permitted a conclusion as to the capacities of this peptide to retain activity and reduce growth of l. monocytogenes egde. moreover, the use of structural variants for the first time in vivo and the reductions of their activities confirmed the im ... | 2010 | 19841145 |
| tnfr2-deficient memory cd8 t cells provide superior protection against tumor cell growth. | tnf receptor-2 (tnfr2) plays a critical role in promoting the activation and survival of naive t cells during the primary response. interestingly, anti-cd3 plus il-2 activated tnfr2(-/-) cd8 t cells are highly resistant to activation-induced cell death (aicd), which correlates with high expression levels of prosurvival molecules such as bcl-2, survivin, and cd127 (il-7ralpha). we determined whether the resistance of activated tnfr2(-/-) cd8 t cells to aicd contributes to more effective protectio ... | 2009 | 19841176 |
| fat content increases the lethality of ultra-high-pressure homogenization on listeria monocytogenes in milk. | listeria monocytogenes ccug 15526 was inoculated at a concentration of approximately 7.0 log(10) cfu/ml in milk samples with 0.3, 3.6, 10, and 15% fat contents. milk samples with 0.3 and 3.6% fat content were also inoculated with a lower load of approximately 3.0 log(10) cfu/ml. inoculated milk samples were subjected to a single cycle of ultra-high-pressure homogenization (uhph) treatment at 200, 300, and 400 mpa. microbiological analyses were performed 2 h after the uhph treatments and after 5, ... | 2009 | 19841200 |
| preexposure to repeated low doses of zymosan increases the susceptibility to pulmonary infection in rats. | chronic exposure to low levels of mold has been reported to increase susceptibility to respiratory infections. in the current study, the authors investigate the lungs' ability to clear an infection after repeated low-dose zymosan exposure. exposure was conducted at a zymosan dose of 0.6 mg/kg body weight (bw) of rat, for a total of 4 doses, via intratracheal instillation during a 2 week period. treated animals were allowed to recover for 1 week before pulmonary inoculation with listeria monocyto ... | 2009 | 19842846 |
| pediocin production in milk by pediococcus acidilactici in co-culture with streptococcus thermophilus and lactobacillus delbrueckii subsp. bulgaricus. | the production of pediocin in milk by pediococcus acidilactici was evaluated in co-culture with the dairy fermentation cultures streptococcus thermophilus and lactobacillus delbrueckii ssp. bulgaricus. the cultures were tested singly and in different combinations in milk (0 or 2% fat content) during incubation at 40 degrees c for up to 10 h. cell-free milk samples taken every 60 min were tested for bacteriocin activity against listeria monocytogenes. pediocin activity was not detectable when p. ... | 2010 | 19844751 |
| real-time pcr detection of five prevalent bacteria causing acute meningitis. | | 2009 | 19845537 |
| selenium deficiency impairs host innate immune response and induces susceptibility to listeria monocytogenes infection. | susceptibility or resistance to infection with listeria monocytogenes correlates with selenium (se) deficiency in response to infection. | 2009 | 19852827 |
| listeria monocytogenes acta is a key player in evading autophagic recognition. | autophagy is a pivotal bulk degradation system that eliminates undesirable molecules, damaged organelles, and misfolded protein aggregates in response to diverse stimuli, including infection. autophagy acts to limit intracellular microbial growth but intracellular pathogens have evolved strategies to subvert host autophagic responses for their survival. we found that listeria monocytogenes acta, a surface protein required for actin polymerization and actin-based bacterial motility, plays a pivot ... | 2009 | 19855178 |
| bioactive alginate coatings to control listeria monocytogenes on cold-smoked salmon slices and fillets. | the relatively high incidence of listeria monocytogenes in cold smoked salmon (css) is of concern as css is a ready-to-eat product. no post-processing measures are currently available to control this pathogen in css. the objective of this study was to develop an effective antimicrobial edible coating containing organic salts to control the growth of l. monocytogenes in css slices and fillets. an in-house made formulation consisting of sodium lactate (sl, 0-2.4%) and sodium diacetate (sd, 0-0.25% ... | 2010 | 19861230 |
| fabh selectivity for anteiso branched-chain fatty acid precursors in low-temperature adaptation in listeria monocytogenes. | gram-positive bacteria, including listeria monocytogenes, adjust membrane fluidity by shortening the fatty acid chain length and increasing the proportional production of anteiso fatty acids at lower growth temperatures. the first condensation reaction in fatty acid biosynthesis is carried out by beta-ketoacyl-acyl carrier protein synthase iii (fabh), which determines the type of fatty acid produced in bacteria. here, we measured the initial rates of fabh-catalyzed condensation of malonyl-acyl c ... | 2009 | 19863661 |
| the in vitro antibiofilm activity of selected culinary herbs and medicinal plants against listeria monocytogenes. | the antibiofilm activity of extracts obtained from selected herbs, spices, beverages and commercially important medicinal plants was investigated on listeria monocytogenes. | 2010 | 19874481 |
| the use of chromogenic bacteria as coloured substitutes for pathogens: a simple strategy during design and development of a new method for sample pretreatment. | in the present study, chromogenic (red) bacteria were used to simulate actual target bacteria during set-up and optimization of an isolation process of bacteria, designed for food samples. isolation of bacteria from food in the context of molecular biological detection of food pathogens is a multistep process. development of such a separation method requires continuous monitoring of the location of the presumable targets in the sample tubes. therefore, red-coloured pigmented bacteria were used a ... | 2010 | 19874486 |
| assessment of survival of listeria monocytogenes, salmonella infantis and enterococcus faecalis artificially inoculated into experimental waste or compost. | to evaluate survival of pathogenic strains, listeria monocytogenes and salmonella infantis and a sanitation indicator enterococcus faecalis in composts at different stages of the composting process and during storage. | 2010 | 19878526 |
| [prevalence of listeria monocytogenes in fresh tomatoes (lycopersicum esculentum) and coriander (coriandrum sativum) in three markets of valencia, venezuela]. | the incidence of l. monocytogenes in tomatoes and coriander obtained from three different markets, during eight weeks were determined. 192 samples were evaluated: 96 of tomatoes, and 96 of coriander. the isolation of l. monocytogenes was performed using covenin 3718:2001. the data were analyzed by spss version 12.0. kolmogorov smirnov, mann whitney u, kruskal wallis u test; spearman's correlation were applied, and p<0.05 significance level was aplied. it was not found significant differences bet ... | 2009 | 19886518 |
| risk of fetal mortality after exposure to listeria monocytogenes based on dose-response data from pregnant guinea pigs and primates. | one-third of the annual cases of listeriosis in the united states occur during pregnancy and can lead to miscarriage or stillbirth, premature delivery, or infection of the newborn. previous risk assessments completed by the food and drug administration/the food safety inspection service of the u.s. department of agriculture/the centers for disease control and prevention (fda/usda/cdc) and food and agricultural organization/the world health organization (fao/who) were based on dose-response data ... | 2009 | 19886944 |
| cutting edge: memory cd8 t cell compartment grows in size with immunological experience but nevertheless can lose function. | the size of the adaptive immune system is considered to be kept constant by the attrition of pre-existing memory. however, recently it was shown that the cd8 memory compartment can grow in size and the number of pre-existing memory is largely preserved, predicting that pre-existing immunity should be maintained (vezys et al.; nature 457: 196-199). experimental proof for this assumption is still lacking. we address this question in the listeria monocytogenes (l.m.) infection model and confirm the ... | 2009 | 19890045 |
| listeria monocytogenes does not survive ingestion by acanthamoeba polyphaga. | listeria monocytogenes is a ubiquitous bacterium capable of infecting humans, particularly pregnant women and immunocompromised individuals. although the intracellular invasion and pathogenesis of listeriosis in mammalian tissues has been well studied, little is known about the ecology of l. monocytogenes , and in particular the environmental reservoir for this bacterium has not been identified. this study used short-term co-culture at 15, 22 and 37 degrees c to examine the interaction of l. mon ... | 2010 | 19892759 |
| the sos response of listeria monocytogenes is involved in stress resistance and mutagenesis. | the sos response is a conserved pathway that is activated under certain stress conditions and is regulated by the repressor lexa and the activator reca. the food-borne pathogen listeria monocytogenes contains reca and lexa homologues, but their roles in listeria have not been established. in this study, we identified the sos regulon in l. monocytogenes by comparing the transcription profiles of a wild-type strain and a deltareca mutant strain after exposure to the dna-damaging agent mitomycin c. ... | 2010 | 19892760 |
| oleanolic acid and ursolic acid affect peptidoglycan metabolism in listeria monocytogenes. | the plant pentacyclic triterpenoids, oleanolic and ursolic acids, inhibit the growth and survival of many bacteria, particularly gram-positive species, including pathogenic ones. the effect of these compounds on the facultative human pathogen listeria monocytogenes was examined. both acids affected cell morphology and enhanced autolysis of the bacterial cells. autolysis of isolated cell walls was inhibited by oleanolic acid, but the inhibitory activity of ursolic acid was less pronounced. both c ... | 2010 | 19894138 |
| chemical composition and antimicrobial and antioxidant activities of mentha (longifolia l. and viridis) essential oils. | the study was aimed to investigate essential oil chemical composition (gas chromatography/flame ionization detection [gc-fid] and gas chromatography/mass spectrometry [gc-ms]) and antioxidant (1,1-diphenyl-2-picrylhydrazyl free radical (dpph) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate [abts] assays) and antimicrobial (gram-positive and gram-negative bacteria, fungi, and yeast) activities of essential oils extracted from leaves of mentha longifolia l. and mentha viridis. gc-ms analysis ... | 2009 | 19895481 |
| effects of allspice, cinnamon, and clove bud essential oils in edible apple films on physical properties and antimicrobial activities. | essential oils (eos) derived from plants are rich sources of volatile terpenoids and phenolic compounds. such compounds have the potential to inactivate pathogenic bacteria on contact and in the vapor phase. edible films made from fruits or vegetables containing eos can be used commercially to protect food against contamination by pathogenic bacteria. eos from cinnamon, allspice, and clove bud plants are compatible with the sensory characteristics of apple-based edible films. these films could e ... | 2009 | 19895483 |
| antibacterial effects of allspice, garlic, and oregano essential oils in tomato films determined by overlay and vapor-phase methods. | physical properties as well as antimicrobial activities against escherichia coli o157:h7, salmonella enterica, and listeria monocytogenes of allspice, garlic, and oregano essential oils (eos) in tomato puree film-forming solutions (tpffs) formulated into edible films at 0.5% to 3% (w/w) concentrations were investigated in this study. antimicrobial activities were determined by 2 independent methods: overlay of the film on top of the bacteria and vapor-phase diffusion of the antimicrobial from th ... | 2009 | 19895486 |
| safety and functional aspects of preselected enterococci for probiotic use in iberian dry-fermented sausages. | the purpose of this study was to investigate enterococci for potential probiotic use in iberian dry-fermented sausages. a total of 15 strains isolated from iberian dry-fermented sausages, human feces, and pig feces were evaluated for their safety and functional characteristics including biogenic amine (ba) production, antibiotic susceptibility, hemolysis, virulence determinants, cell adhesion, and antimicrobial activity against foodborne pathogens. the strain enterococcus faecium se906 was able ... | 2009 | 19895487 |
| influence of sublethal concentrations of common disinfectants on expression of virulence genes in listeria monocytogenes. | listeria monocytogenes is a food-borne human pathogen that causes listeriosis, a relatively rare infection with a high fatality rate. the regulation of virulence gene expression is influenced by several environmental factors, and the aim of the present study was to determine how disinfectants used routinely in the food industry affect the expression of different virulence genes in l. monocytogenes when added at sublethal concentrations. an agar-based assay was developed to screen the effect of d ... | 2010 | 19897753 |
| galleria mellonella as a model system for studying listeria pathogenesis. | essential aspects of the innate immune response to microbial infection are conserved between insects and mammals. this has generated interest in using insects as model organisms to study host-microbe interactions. we used the greater wax moth galleria mellonella, which can be reared at 37 degrees c, as a model host for examining the virulence potential of listeria spp. here we report that galleria is an excellent surrogate model of listerial septic infection, capable of clearly distinguishing be ... | 2010 | 19897755 |
| survival of listeria monocytogenes in simulated gastrointestinal system and transcriptional profiling of stress- and adhesion-related genes. | food ingestion is the major route of exposure to the important human pathogen listeria monocytogenes. an in vitro gastrointestinal model was used to (1) compare the survival rates of l. monocytogenes strains of serotypes 1/2a, 1/2c, and 4b; and (2) examine the transcription of stress- and adhesion-related genes after exposure to the conditions similar to those encountered in the mouth, stomach, and small intestine. none of the l. monocytogenes strains investigated could survive in the gastric ju ... | 2010 | 19899961 |
| wild boars as an important reservoir for foodborne pathogens. | one hundred fifty-three wild boars shot in the canton of geneva, switzerland, were studied for the occurrence of foodborne pathogens. tonsils and fecal samples of the animals were examined using real-time polymerase chain reaction, enzyme-linked fluorescent immunoassay, and cultural methods. the detection rate of salmonella spp., yersinia enterocolitica, yersinia pseudotuberculosis, stx-positive escherichia coli, and listeria monocytogenes was 12%, 35%, 20%, 9%, and 17%, respectively, when tonsi ... | 2010 | 19899962 |
| ligand-mediated dimerization of the met receptor tyrosine kinase by the bacterial invasion protein inlb. | the listeria monocytogenes surface protein inlb mediates bacterial invasion into host cells by activating the human receptor tyrosine kinase met. so far, it is unknown how inlb or the physiological met ligand hepatocyte growth factor/scatter factor causes met dimerization, which is considered a prerequisite for receptor activation. we determined two new structures of inlb, revealing a recurring, antiparallel, dimeric arrangement, in which the two protomers interact through the convex face of the ... | 2010 | 19900460 |
| tetraspanin cd81 is required for listeria monocytogenes invasion. | listeria monocytogenes is an intracellular bacterial pathogen that invades epithelial cells by subverting two cellular receptors, e-cadherin and met. we recently identified type ii phosphatidylinositol 4-kinases alpha and beta (pi4kiialpha and pi4kiibeta) as being required for bacterial entry downstream of met. in this work, we investigated whether tetraspanins cd9, cd63, and cd81, which figure among the few described molecular partners of pi4kiialpha, function as molecular adaptors recruiting p ... | 2010 | 19901060 |
| gammadelta t lymphocytes producing ifngamma and il-17 in response to candida albicans or mycobacterial antigens: possible implications for acute and chronic inflammation. | t lymphocytes bearing the gammadelta t cell receptor are known to play an important role in the first-line defense against viral, bacterial and fungal pathogens. two main subsets of gammadelta t cells are known, showing distinct functional behaviour: vdelta2 t lymphocytes, circulating in the peripheral blood, are involved in the response to mycobacterial infections and certain viruses, including coxsakie virus b3 and herpes simplex virus type 2. vdelta1 t cells are resident in the mucosal-associ ... | 2009 | 19903136 |
| assuring growth inhibition of listerial contamination during processing and storage of traditional greek graviera cheese: compliance with the new european union regulatory criteria for listeria monocytogenes. | the current microbiological regulatory criteria in the european union specify a maximum listeria monocytogenes population of 100 cfu/g allowable in ready-to-eat foods provided the product will not exceed this limit throughout its shelf life. the aim of this study was to validate the manufacturing method for traditional greek graviera cheese produced from thermized milk. initial challenge experiments evaluated the fate of inoculated l. monocytogenes (ca. 4 log cfu/ml, three-strain cocktail) in th ... | 2009 | 19903387 |
| efficacy of chlorine dioxide against listeria monocytogenes in brine chilling solutions. | chilled brine solutions are used by the food industry to rapidly cool ready-to-eat meat products after cooking and before packaging. chlorine dioxide (clo(2)) was investigated as an antimicrobial additive to eliminate listeria monocytogenes. several experiments were performed using brine solutions made of sodium chloride (nacl) and calcium chloride (cacl(2)) inoculated with l. monocytogenes and/or treated with 3 ppm of clo(2). first, 10 and 20% cacl(2) and nacl solutions (ph 7.0) were inoculated ... | 2009 | 19903388 |
| morphology, release characteristics, and antimicrobial effect of nisin-loaded electrospun gelatin fiber mat. | gelatin electrospun (e-spun) fiber mats containing nisin were produced by electrostatic spinning of gelatin-nisin in 70% (vol/vol) acetic acid aqueous solutions. varying nisin loading concentration (0 to 3% [wt/wt]) did not affect the fiber average diameter, whereas increasing gelatin concentration from 20 to 24% (wt/vol) caused an increase in the average diameter. all nisin-loaded gelatin e-spun fiber mats demonstrated inhibition against lactobacillus plantarum tistr 850. however, all fiber mat ... | 2009 | 19903391 |
| prevalence and molecular diversity of listeria monocytogenes in retail establishments. | as our understanding of listeria monocytogenes transmission in retail and deli operations is limited, we conducted a cross-sectional study of l. monocytogenes contamination patterns in 121 retail establishments, using testing of food and environmental samples and subtype analysis (ribotyping) of l. monocytogenes isolates. seventy-three (60%) establishments had at least one sample that tested positive for l. monocytogenes; 5 (2.7%) of the 183 food and 151 (13.0%) of the 1,161 environmental sample ... | 2009 | 19903398 |
| pkctheta is required for alloreactivity and gvhd but not for immune responses toward leukemia and infection in mice. | when used as therapy for hematopoietic malignancies, allogeneic bm transplantation (bmt) relies on the graft-versus-leukemia (gvl) effect to eradicate residual tumor cells through immunologic mechanisms. however, graft-versus-host disease (gvhd), which is initiated by alloreactive donor t cells that recognize mismatched major and/or minor histocompatibility antigens and cause severe damage to hematopoietic and epithelial tissues, is a potentially lethal complication of allogeneic bmt. to enhance ... | 2009 | 19907075 |
| multilocus sequence typing of outbreak-associated listeria monocytogenes isolates to identify epidemic clones. | listeria monocytogenes is a foodborne pathogen found in a wide variety of environments. subtype characterization of l. monocytogenes isolates from listeriosis outbreaks that have occurred over the last three decades has suggested that a number of outbreaks were caused by a small number of l. monocytogenes epidemic clones (ecs). in this study we compared the prevalence, ecology, and phylogenetic position of outbreak-associated isolates and non-outbreak-associated isolates to probe the evolutionar ... | 2010 | 19911921 |
| re-evaluation, optimization, and multilaboratory validation of the pulsenet-standardized pulsed-field gel electrophoresis protocol for listeria monocytogenes. | the pulsenet methods development and validation laboratory began a re-evaluation of the standardized pulsed-field gel electrophoresis (pfge) protocols with the goal of optimizing their overall performance and robustness. herein, we describe a stepwise evaluation of the pulsenet-standardized pfge protocol for listeria monocytogenes that led to the modification of several steps which significantly improved the overall appearance and reproducibility of the resulting pfge data. these improvements in ... | 2010 | 19911934 |
| a listeria monocytogenes strain is still virulent despite nonfunctional major virulence genes. | the low-virulence listeria monocytogenes strains have been previously assigned to 4 phenotypic groups. this study aimed to characterize the a23 strain, which exhibits a pulsed-field gel electrophoresis profile specific to low-virulence strains. this strain has the same causal mutations as the group iii strains and a supplementary mutation in the mpl gene, leading to the absence of internalin a expression and the presence of inactive internalin b, phosphatidyl-inositol phospholipase c, and phosph ... | 2009 | 19911993 |
| pregnancy does not deter the development of a potent maternal protective cd8+ t-cell acquired immune response against listeria monocytogenes despite preferential placental colonization. | listeria monocytogenes (lm) preferentially colonizes the placenta and causes fetal loss and systemic disease during pregnancy. as systemic cd8+ t-cell memory is critical in controlling lm infection, we addressed the issue as to whether it is modulated during pregnancy. | 2010 | 19912156 |
| quantification of listeria monocytogenes in minimally processed leafy vegetables using a combined method based on enrichment and 16s rrna real-time pcr. | modern lifestyle markedly changed eating habits worldwide, with an increasing demand for ready-to-eat foods, such as minimally processed fruits and leafy greens. packaging and storage conditions of those products may favor the growth of psychrotrophic bacteria, including the pathogen listeria monocytogenes. in this work, minimally processed leafy vegetables samples (n = 162) from retail market from ribeirão preto, são paulo, brazil, were tested for the presence or absence of listeria spp. by the ... | 2010 | 19913686 |
| inactivation of escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri on spinach leaves by x-ray. | several recent foodborne disease outbreaks associated with leafy green vegetables, including spinach, have been reported. x-ray is a non-thermal technology that has shown promise for reducing pathogenic and spoilage bacteria on spinach leaves. inactivation of inoculated escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri on spinach leaves using x-ray at different doses (0.1, 0.2, 0.3, 0.5, 0.75, 1.0, 1.5 and 2.0 kgy) was studied. the effect of x-ray on col ... | 2010 | 19913687 |
| evaluation of an enterocin as-48 enriched bioactive powder obtained by spray drying. | enterocin as-48 is a cationic cyclic bacteriocin produced by enterococcus faecalis with broad bactericidal activity. currently we are assaying the efficacy of as-48 as biopreservative in foods. in this work we have applied the spray drying process to different as-48 liquid samples to obtain active dried preparations. we have also assayed different methods, heat, uv irradiation and filtration, to inactivate/remove the as-48 producer cells from the samples. best results were obtained for the sampl ... | 2010 | 19913693 |
| sodium lactate, sodium diacetate and pediocin: effects and interactions on the thermal inactivation of listeria monocytogenes on bologna. | the effects and interactions of temperature (56.3-60 degrees c), sodium lactate (sl; 0-4.8%), sodium diacetate (sd; 0-0.25%) and pediocin (0-10,000 au) on listeria monocytogenes on bologna were studied and a predictive inactivation model was developed. bologna was manufactured with different sl/sd concentrations in the formulation, dipped in pediocin solution and treated at different temperatures using combinations of parameters determined by central composite design. d-values were calculated an ... | 2010 | 19913694 |
| amplified fragment length polymorphism and multi-locus sequence typing for high-resolution genotyping of listeria monocytogenes from foods and the environment. | standardized tools for typing listeria monocytogenes isolates are required in epidemiological surveys investigating food-borne disease outbreaks and in the food-processing environment to identify the sources of contamination and routes by which the organisms are spread. in this survey amplified fragment length polymorphism (aflp) and multi-locus sequence typing (mlst) have been used to study 103 l. monocytogenes isolates from food and environmental sources. a total of 62 aflp types and 66 mlst s ... | 2010 | 19913699 |
| effects of x-ray radiation on escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri inoculated on shredded iceberg lettuce. | the main goal of this investigation was to study the efficacy of x-ray doses (0.1, 0.2, 0.3, 0.5, 0.75, 1.0, 1.5 and 2.0 kgy) on inoculated escherichia coli o157: h7, listeria monocytogenes, salmonella enterica and shigella flexneri on shredded iceberg lettuce. the second goal was to study the effect of x-ray on the inherent microflora counts and visual color of shredded iceberg lettuce during storage at 4 degrees c for 30 days. treatment with 1.0 kgy x-ray significantly reduced the population o ... | 2010 | 19913700 |
| fate of listeria monocytogenes during freezing, thawing and home storage of frankfurters. | little information is available regarding the fate of listeria monocytogenes during freezing, thawing and home storage of frankfurters even though recent surveys show that consumers regularly store unopened packages in home freezers. this study examined the effects of antimicrobials, refrigerated storage, freezing, thawing method, and post-thawing storage (7 degrees c) on l. monocytogenes on frankfurters. inoculated (2.1 log cfu/cm(2)) frankfurters formulated without (control) or with antimicrob ... | 2010 | 19913705 |
| effect of nanovesicle-encapsulated nisin on growth of listeria monocytogenes in milk. | commercial nisin was encapsulated in nanovesicles (mean diameter 140 nm) prepared from partially purified soy lecithin. nisin-loaded liposomes and unencapsulated (free) nisin were initially tested in bhi medium and skim milk inoculated with listeria monocytogenes and incubated for 48 h at 30 degrees c. at such abuse temperature conditions, free nisin showed better inhibitory than the liposomal counterparts. subsequently, the effect of encapsulated or free nisin was evaluated in combination with ... | 2010 | 19913710 |
| polymorphism of acta gene is not related to in vitro virulence of listeria monocytogenes. | listeria monocytogenes is a foodborne pathogen which is able to cause serious disease both in humans and in animals. several studies have demonstrated variations in the levels of virulence among l. monocytogenes strains. invasion and growth ability of l. monocytogenes into cultured cells have been used to evaluate its pathogenicity. in particular, invasiveness and growth ability have been typically investigated using hela cell line. this study aimed to provide further insights on the virulence p ... | 2010 | 19913932 |
| a trans-acting riboswitch controls expression of the virulence regulator prfa in listeria monocytogenes. | riboswitches are rna elements acting in cis, controlling expression of their downstream genes through a metabolite-induced alteration of their secondary structure. here, we demonstrate that two s-adenosylmethionine (sam) riboswitches, srea and sreb, can also function in trans and act as noncoding rnas in listeria monocytogenes. srea and sreb control expression of the virulence regulator prfa by binding to the 5'-untranslated region of its mrna. absence of the sam riboswitches srea and sreb incre ... | 2009 | 19914169 |
| microbiological analysis of composts produced on south carolina poultry farms. | the purpose of this study was to determine whether the methods used in compost operations of small and medium-sized poultry farms resulted in the production of an amendment free of foodborne pathogens. | 2010 | 19922596 |
| defensin from disk abalone haliotis discus discus: molecular cloning, sequence characterization and immune response against bacterial infection. | gene-encoded antimicrobial peptides (amps) serve a major role in host defense systems against pathogens. in this study, cdna of a new mollusk defensin was identified from a normalized cdna library constructed from whole tissues of disk abalone. abalone defensin peptide (pro-defensin) has a 198-bp coding sequence comprised of a putative 66 amino acids with a mature defensin consisting of 48 amino acid residues. the presence of an invertebrate defensin family domain, an arrangement of six cysteine ... | 2010 | 19922800 |
| il-23 is required for protection against systemic infection with listeria monocytogenes. | listeria monocytogenes (lm) is a gram-positive, intracellular bacterium that can induce spontaneous abortion, septicemia, and meningitis. although it is known that neutrophils are required for elimination of the bacteria and for survival of the host, the mechanisms governing the recruitment of neutrophils to lm-infected tissues are not fully understood. we demonstrate here that il-23 and the il-17 receptor a (il-17ra), which mediates both il-17a and il-17f signaling, are necessary for resistance ... | 2009 | 19923464 |
| [a case of listeria meningitis showed high levels of adenosine deaminase in cerebrospinal fluid]. | an 83-year-old woman developed high fever and headache for four days. she had disturbance of consciousness and was admitted to our hospital. a lumbar puncture was performed and cerebrospinal fluid (csf) analysis revealed a white blood cell count of 268/mm3 and culture became positive for listeria monocytogenes. six days after admission, the adenosine deaminase (ada) level in csf markedly elevated (43.3 iu/l) with pleocytosis, but a negative pcr test for tuberculosis in csf, sputum, gastric fluid ... | 2009 | 19928691 |
| infectious encephalitis in france in 2007: a national prospective study. | encephalitis is associated with significant mortality and morbidity, but its cause remains largely unknown. we designed a national prospective study in france in 2007 to describe patients with encephalitis, investigate the etiologic diagnosis of encephalitis, and assess risk factors associated with death. | 2009 | 19929384 |
| encephalitis: why we need to keep pushing the envelope. | | 2009 | 19929385 |
| nisin-induced expression of pediocin in dairy lactic acid bacteria. | to test whether a single vector, nisin-controlled expression (nice) system could be used to regulate expression of the pediocin operon in streptococcus thermophilus, lactococcus lactis subsp. lactis and lactobacillus casei. | 2010 | 19929951 |
| comparison of conventional and molecular methods for the routine confirmation of listeria monocytogenes in milk products produced domestically in croatia. | conventional methods for the identification of listeria in foodstuffs are generally cumbersome and time consuming. the use of primary enrichment in half strength fraser broth and the use of palcam agar were assessed in comparison with api listeria and polymerase chain reaction (pcr) for their ability to accurately detect and confirm the presence of list. monocytogenes in milk products. the aim of our work was to detect list. monocytogenes in domestic unpasteurised milk, fresh cheese and cream of ... | 2010 | 19930757 |
| quantification of host-microbe interactions by automated fluorescence microscopy. | we describe an automated fluorescence microscopy-based assay that quantifies the invasion of mammalian cells by intracellular pathogens. pathogens associated with host cell surfaces, intracellular pathogens and mammalian cells are directly counted based on their specific fluorescent labeling. such approach utilizes automated image acquisition and processing, and is thus ideally suited for high-throughput analyses. this method was validated using listeria monocytogenes as a model intracellular pa ... | 2010 | 19931271 |
| effect of growth and recovery temperatures on pressure resistance of listeria monocytogenes. | experimental conditions can affect the outcome of bacterial stress-tolerance assays. growth conditions that optimize microbial recovery should be established to help evaluate the effectiveness of treatment conditions for food safety. the objectives of this study were to determine the effects of growth and recovery temperatures on pressure resistance of early stationary-phase listeria monocytogenes in milk. the tested conditions were the following: (1) l. monocytogenes was grown at various temper ... | 2010 | 19931930 |
| molecular and genetic characterization of a novel bacteriocin locus in enterococcus avium isolates from infants. | enterococci are among the most common human intestinal lactic acid bacteria, and they are known to produce bacteriocins. in this study, fecal enterococci were isolated from infants and screened for bacteriocin production. bacteriocin-producing enterococcus avium isolates were obtained, and a new pediocin-like bacteriocin was purified and characterized. this bacteriocin, termed avicin a, was found to be produced by isolates from two healthy infants. it was purified to homogeneity from culture sup ... | 2010 | 19933345 |
| transcriptomic response of listeria monocytogenes to iron limitation and fur mutation. | iron is required by almost all bacteria, but concentrations above physiological levels are toxic. in bacteria, intracellular iron is regulated mostly by the ferric uptake regulator, fur, or a similar functional protein. iron limitation results in the regulation of a number of genes, especially those involved in iron uptake. a subset of these genes is the fur regulon under the control of fur. in the present study, we have identified fur- and iron-regulated genes in listeria monocytogenes by dna m ... | 2010 | 19933349 |
| tumor progression locus 2 (map3k8) is critical for host defense against listeria monocytogenes and il-1 beta production. | tumor progression locus 2 (tpl2, also known as map3k8 and cot) is a serine-threonine kinase critical in innate immunity, linking toll-like receptors (tlrs) to tnf production through its activation of erk. tpl2(-/-) macrophages have abrogated tnf production but overproduce il-12 in response to tlr ligands. despite enhanced il-12 production, tpl2(-/-) t cells have impaired ifn-gamma production. therefore, the role of tpl2 in a bona fide bacterial infection where all of these cytokines are importan ... | 2009 | 19933865 |
| different routes of bacterial infection induce long-lived th1 memory cells and short-lived th17 cells. | we used a sensitive method based on tetramers of peptide and major histocompatibility complex ii (pmhcii) to determine whether cd4(+) memory t cells resemble the t helper type 1 (t(h)1) and interleukin 17 (il-17)-producing t helper (t(h)17) subsets described in vitro. intravenous or intranasal infection with listeria monocytogenes induced pmhcii-specific cd4(+) naive t cells to proliferate and produce effector cells, about 10% of which resembled t(h)1 or t(h)17 cells, respectively. t(h)1 cells w ... | 2010 | 19935657 |
| contribution of cysteine residue to the properties of listeria monocytogenes listeriolysin o. | listeriolysin (llo) is the key virulence factor critical for listeria monocytogenes pathogenesis. listerial cytolysin belongs to the family of cholesterol-dependent cytolysins (cdcs), a group of pore-forming toxins produced by related gram-positive bacteria. most cdcs contain a cysteine residue in the conserved undecapeptide - a sequence that is highly preserved among this group of proteins. substitutions of cysteine do not always lead to loss of hemolytic activity, questioning the purpose of su ... | 2009 | 19935887 |
| monitoring bacterial indicators and pathogens in cattle feedlot waste by real-time pcr. | quantitative microbial health risk assessment requires accurate enumeration of pathogens in hazard-containing matrices as part of the risk characterization process. as part of a risk management-oriented study of cattle feedlot waste contaminants, we investigated the utility of quantitative real-time pcr (qpcr) for surveying the microbial constituents of different faecal wastes. the abundance of escherichia coli and enterococci were first estimated in five cattle feedlot waste types from five loc ... | 2010 | 19939430 |
| dcs in mouse models of intracellular bacterial infection. | direct infection of antigen-presenting cells (apcs) with living intracellular bacteria may influence the early innate immune response as well as the following t-cell response. revealing the identity of primarily targeted cells during infection is therefore an important task, which requires sensitive methods for the intracellular detection of bacteria before their extensive replication. determination of colony-forming units (cfu) combines - in contrast to microscopy-based methods - a high sensiti ... | 2010 | 19941122 |
| defining a role for hfq in gram-positive bacteria: evidence for hfq-dependent antisense regulation in listeria monocytogenes. | small trans-encoded rnas (srnas) modulate the translation and decay of mrnas in bacteria. in gram-negative species, antisense regulation by trans-encoded srnas relies on the sm-like protein hfq. in contrast to this, hfq is dispensable for srna-mediated riboregulation in the gram-positive species studied thus far. here, we provide evidence for hfq-dependent translational repression in the gram-positive human pathogen listeria monocytogenes, which is known to encode at least 50 srnas. we show that ... | 2010 | 19942685 |
| immobilization of bacteriophages on modified silica particles. | bacteriophages are selective anti-bacterial agents, which are receiving increasing acceptance by regulatory agencies for use both in the food industry and in clinical settings for biocontrol. while immobilized phage could be particularly useful to create antimicrobial surfaces, current immobilization strategies require chemical bioconjugation to surfaces or more difficult processes involving modification of their head proteins to express specific binding moieties, for example, biotin or cellulos ... | 2010 | 19945158 |
| the overgrowth of listeria monocytogenes by other listeria spp. in food samples undergoing enrichment cultivation has a nutritional basis. | the isolation of listeria monocytogenes from food is carried out using a double enrichment. in cases where multiple listeria species are present within the original sample, l. monocytogenes can be overgrown during enrichment by other species of listeria present in the original sample. from a practical perspective, this can result in a false negative or complicate the ability of public health investigators to match food and clinical isolates. we have further investigated this phenomenon by analys ... | 2010 | 19945759 |
| diverse cadmium resistance determinants in listeria monocytogenes isolates from the turkey processing plant environment. | two different cada cadmium resistance determinants (cada1, first identified in tn5422, and cada2, associated with plm80) were detected among cadmium-resistant listeria monocytogenes strains from turkey processing plants. prevalence of cada1 versus cada2 was serotype associated. cadmium-resistant isolates that were also resistant to benzalkonium chloride (bc) were more likely to harbor cada2 alone or together with cada1 than isolates that were cadmium resistant but bc susceptible. | 2010 | 19948870 |
| il-15 regulates both quantitative and qualitative features of the memory cd8 t cell pool. | memory t cells are critical for immunity to various intracellular pathogens. recent studies have indicated that cd8 secondary memory cells, induced by prime-boost approaches, show enhanced protective function compared with primary memory cells and exhibit phenotypic and functional characteristics that distinguish them from primary memory cells. however, little is known about the cytokine requirements for generation and maintenance of boosted memory cd8 t cells. we studied the role of il-15 in de ... | 2010 | 19949092 |
| antilisterial activity of a broad-spectrum bacteriocin, enterocin lr/6 from enterococcus faecium lr/6. | enterocin lr/6, a purified bacteriocin, exhibited broad inhibitory spectrum both against related as well as some food-borne pathogens such as listeria monocytogenes, yersinia enterocolitica, aeromonas sp., shigella sp., and bacillus licheniformis. in this investigation, we have focused on l. monocytogenes as the target organism, as it is not only an important pathogen but can also survive over a wide range of environmental conditions such as refrigeration temperature, low ph, and high-salt conce ... | 2010 | 19957209 |
| reduction of listeria monocytogenes on the surface of fresh channel catfish fillets by bacteriophage listex p100. | bacteriophage listex p100 (phage p100) was approved by the u.s. food and drug administration and u.s. department of agriculture's food safety and inspection service for listeria monocytogenes control on both raw and ready-to-eat food products. in this article, we present the proof of concept on the influence of phage dose, phage contact time, and storage temperature on the listericidal activity of phage p100 in reducing the l. monocytogenes loads on the surface of fresh channel catfish fillet. t ... | 2010 | 19958102 |
| evaluation of a multiplex pcr assay as an alternative method for listeria monocytogenes serotyping. | listeria monocytogenes serotyping is commonly used as the first level of characterisation in the epidemiological surveillance of food and clinical isolates and is therefore widely accepted. the aim of this study was to define a scheme for multiplex molecular serotyping of l. monocytogenes based on a previously described pcr assay and then to evaluate and compare this new procedure with conventional serotyping by agglutination. the study included 1204 listeria strains collected from food products ... | 2010 | 19958798 |
| the role of infection in inflammatory bowel disease: initiation, exacerbation and protection. | inflammatory bowel disease, a collective term for ulcerative colitis and crohn's disease, is a chronic, immune-mediated disease of the gastrointestinal tract that develops in genetically susceptible individuals. the role of infection in the development of inflammatory bowel disease is underscored by various clinical observations, such as the delayed age of onset, suggesting that childhood exposure to pathogens is essential, and the clinical improvement that follows decreasing bacterial intestina ... | 2009 | 19960852 |
| short-term inhibition of p53 combined with keratinocyte growth factor improves thymic epithelial cell recovery and enhances t-cell reconstitution after murine bone marrow transplantation. | myeloablative conditioning before bone marrow transplantation (bmt) results in thymic epithelial cell (tec) injury, t-cell immune deficiency, and susceptibility to opportunistic infections. conditioning regimen-induced tec damage directly contributes to slow thymopoietic recovery after bmt. keratinocyte growth factor (kgf) is a tec mitogen that stimulates proliferation and, when given before conditioning, reduces tec injury. some tec subsets are refractory to kgf and functional t-cell responses ... | 2010 | 19965631 |
| delayed but functional neutrophil extracellular trap formation in neonates. | | 2009 | 19965699 |
| cd4+ t cell regulation of cd25 expression controls development of short-lived effector cd8+ t cells in primary and secondary responses. | both cd4(+) t cell help and il-2 have been postulated to "program" activated cd8(+) t cells for memory cell development. however, the linkage between these two signals has not been well elucidated. here we have studied effector and memory cd8(+) t cell differentiation following infection with three pathogens (listeria monocytogenes, vesicular stomatitis virus, and vaccinia virus) in the absence of both cd4(+) t cells and il-2 signaling. we found that expression of cd25 on antigen-specific cd8(+) ... | 2010 | 19966302 |
| hydrophobization and antimicrobial activity of chitosan and paper-based packaging material. | this study reports the elaboration of water-resistant, antimicrobial, chitosan and paper-based materials as environmentally friendly food packaging materials. two types of papers were coated with chitosan-palmitic acid emulsions or with a blend of chitosan and o,o'-dipalmitoylchitosan (dpct). micromorphology studies showed that inclusion of hydrophobic compounds into the chitosan matrix was enhanced by grafting them onto chitosan and that this led to their penetration of the paper's core. compar ... | 2010 | 19994882 |
| tio2 nanowire bundle microelectrode based impedance immunosensor for rapid and sensitive detection of listeria monocytogenes. | | 2009 | 19995090 |
| multiple innate signaling pathways cooperate with cd40 to induce potent, cd70-dependent cellular immunity. | we have previously shown that toll-like receptor (tlr) agonists cooperate with cd40 to generate cd8 t cell responses exponentially larger than the responses generated with traditional vaccine formulations. we have also shown that combined tlr agonist/anti-cd40 immunization uniquely induces the upregulation of cd70 on antigen bearing dendritic cells (dcs). in contrast, immunization with either a tlr agonist or a cd40 stimulus alone does not significantly increase cd70 expression on dcs. furthermo ... | 2010 | 19995538 |
| purification and characterization of phocaecin pi80: an anti-listerial bacteriocin produced by streptococcus phocae pi80 isolated from the gut of peneaus indicus (indian white shrimp). | a bacteriocin-producing strain pi80 was isolated from the gut of penaeus indicus (indian white shrimp) and identified as streptococcus phocae pi80. the bacteriocin was purified from a culture supernatant to homogeneity as confirmed by tricine sds-page. reverse-phase hplc analysis revealed a single active fraction eluted at 12.94 min, and maldi-tof mass spectrometry analysis showed the molecular mass to be 9.244 kda. this molecular mass does not correspond to previously described streptococcal ba ... | 2009 | 19996693 |
| combined effects of probiotic fermentation and high-pressure extraction on the antioxidant, antimicrobial, and antimutagenic activities of deodeok (codonopsis lanceolata). | this study was designed to evaluate the combined effects of probiotic fermentation and high-pressure extraction (hpe) on the functional properties of codonopsis lanceolata. the ground c. lanceolata samples were anaerobically fermented with lactobacillus acidophilus adh, bifidobacterium longum b6, lactobacillus rhamnosus gg, or lactobacillus paracasei at 37 degrees c for 10 days and subjected to 500 mpa at 50 degrees c for 30 min. the extraction yields of c. lanceolata samples were noticeably inc ... | 2010 | 20000575 |
| the combination of energy-dependent internal adaptation mechanisms and external factors enables listeria monocytogenes to express a strong starvation survival response during multiple-nutrient starvation. | the goal of this study was to characterize the starvation survival response (ssr) of a wild-type listeria monocytogenes 10403s and an isogenic deltasigb mutant strain during multiple-nutrient starvation conditions over 28 days. this study examined the effects of inhibitors of protein synthesis, the proton motive force, substrate level phosphorylation, and oxidative phosphorylation on the ssr of l. monocytogenes 10403s and a deltasigb mutant during multiple-nutrient starvation. the effects of sta ... | 2010 | 20001327 |
| listeriosis: a resurgent foodborne infection. | listeria monocytogenes is the causative agent of human listeriosis, a potentially fatal foodborne infection. clinical manifestations range from febrile gastroenteritis to more severe invasive forms, including sepsis, meningitis, rhombencephalitis, perinatal infections, and abortions. in recent years, an increasing rate of listeriosis has been reported in several european countries. these increases primarily reflect a higher rate of bacteraemic listeriosis in those > or =65 years of age, and are ... | 2010 | 20002687 |
| eevd motif of heat shock cognate protein 70 contributes to bacterial uptake by trophoblast giant cells. | the uptake of abortion-inducing pathogens by trophoblast giant (tg) cells is a key event in infectious abortion. however, little is known about phagocytic functions of tg cells against the pathogens. here we show that heat shock cognate protein 70 (hsc70) contributes to bacterial uptake by tg cells and the eevd motif of hsc70 plays an important role in this. | 2009 | 20003465 |
| penicillin-binding proteins (pbp) and lmo0441 (a pbp-like protein) play a role in beta-lactam sensitivity of listeria monocytogenes. | while seven penicillin-binding proteins (pbps) or pbp-like proteins have been identified either by radiolabelled penicillin binding studies or genomic analysis, only pbp3 has been considered of interest for beta-lactams activity against listeria monocytogenes. herein we reveal that both pbp4 and lmo0441 (a pbp-like protein) play a direct role in cephalosporin activity in l. monocytogenes while pbp4 additionally has a protective affect against both penicillin and carbapenem. | 2009 | 20003484 |
| detection, isolation, and incidence of listeria spp. in small-scale artisan cheese processing facilities: a methods comparison. | environmental sampling, focused on environmental sites with a high probability of contamination, was conducted in eight artisan cheese processing facilities. samples (n = 236) from 86 food contact surfaces and 150 non-food contact surfaces were examined for the presence of listeria spp. by comparing the efficacy of three different primary enrichment media used in five detection and isolation methods. university of vermont broth was the most sensitive primary enrichment medium for the detection o ... | 2009 | 20003731 |
| anti-listeria monocytogenes activity of heat-treated lyophilized pomegranate juice in media and in ground top round beef. | heat treatment can affect antimicrobial activity of plant by-products by altering phenolic content and composition and forming melanoidins. the antilisterial efficacy of heat-treated and unheated lyophilized pomegranate juice (lpj) was determined. the lpj was heated at 100 degrees c for 0, 30, 60, or 120 min and added at 2% (wt/wt) to ground top round beef, which was then cooked and inoculated with individual l. monocytogenes strains. samples of meat stored at 5 degrees c were taken at days 1, 8 ... | 2009 | 20003732 |
| inactivation of listeria monocytogenes on hams shortly after vacuum packaging by spray application of lauric arginate. | this study measured and compared the short-term efficacy levels of lauric arginate (lae) as a postlethality treatment against listeria monocytogenes present on varied surfaces of large-diameter hams. preliminary in vitro work demonstrated a 5-log inactivation of l. monocytogenes in 5,000- and 9,090-ppm lae solutions within 180 min at 4.4 and 23 degrees c. six different whole-muscle ham types were inoculated with l. monocytogenes at ca. 7-log cfu per ham and spray treated with between 15 and 29 m ... | 2009 | 20003733 |
| effect of storage and subsequent reheating on viability of listeria monocytogenes on pork scrapple. | we evaluated the fate of listeria monocytogenes on commercial pork scrapple, a regionally popular, ready-to-eat (rte) meat. we also conducted an informal survey to address consumer practices for storing and reheating scrapple. of the 129 consumers who responded to at least one of the eight questions posed in the survey, about half (46.4%; 52 of 112) considered scrapple rte, the majority (69.7%; 76 of 109) stored it in the refrigerator, and all (100%; 112 of 112) preferred to reheat it prior to c ... | 2009 | 20003735 |
| chicken salad as the source of a case of listeria monocytogenes infection in connecticut. | listeriosis is a severe infection with high morbidity and mortality. we report a fatal case of listeriosis in a patient with a history of crohn's disease who consumed chicken salad purchased from a retail food establishment before developing listeriosis. as part of the regulatory testing programs, the u.s. department of agriculture food safety and inspection service and the florida department of agriculture and consumer affairs found that chicken products from a single food-production establishm ... | 2009 | 20003746 |