| cd8 t cell immunity to plasmodium permits generation of protective antibodies after repeated sporozoite challenge. | individuals living in malaria endemic areas are subject to repeated infections yet fail to develop sterilizing immunity, however, immunization of mice with attenuated sporozoites or subunit vaccines has shown the ability to protect mice against a sporozoite challenge. we recently reported that mice primed with dendritic cells coated with the dominant circumsporozoite cd8 t cell epitope from plasmodium berghei followed by a boost with recombinant listeria monocytogenes expressing the same epitope ... | 2009 | 19712771 |
| usda fsis, fda bam, aoac, and iso culture methods bd bbl chromagar listeria media. | bbl chromagar listeria media (cl) was evaluated for detection of listeria monocytogenes in raw ground beef, smoked salmon, lettuce, and brie cheese. the recovery of l. monocytogenes on cl was compared to the u.s. food and drug administration (fda) bacteriological analytical manual (bam), u.s. department of agriculture (usda) food safety and inspection service (fsis), aoac, and international organization for standardization (iso) reference-plated media using the recommended pre-enrichments and se ... | 2009 | 19714979 |
| preparation of antimicrobial membranes: coextrusion of poly(lactic acid) and nisaplin in the presence of plasticizers. | nisin is a naturally occurring antimicrobial polypeptide and is popularly used in the food and food-packaging industries. nisin is deactivated at temperatures higher than 120 degrees c and, therefore, cannot be directly incorporated into poly(l-lactic acid) (pla), a biomass-derived biodegradable polymer, by coextrusion because pla melts at temperatures around 160 degrees c or above. however, pla can remain in a melt state at temperatures below the t(m) in the presence of lactic acid or other pla ... | 2009 | 19715335 |
| evidence-based semiquantitative methodology for prioritization of foodborne zoonoses. | to prioritize an extended list of food- and water-borne zoonoses to allow food safety authorities to focus on the most relevant hazards in the food chain. | 2009 | 19715429 |
| beta-naphthoflavone causes an ahr-independent inhibition of invasion and intracellular multiplication of listeria monocytogenes in murine hepatocytes. | we recently reported a heretofore unknown role for the aryl hydrocarbon receptor in host resistance to listeriosis in mice. hepatocytes are an important site for listeria monocytogenes multiplication in vivo. in this study, we investigated whether activation of ahr in tib73 murine embryonic hepatocytes affects the ingestion and intracellular multiplication of l. monocytogenes. treatment of tib73 cells with the ahr agonist beta-naphthoflavone (bnf) significantly inhibited the ingestion and intrac ... | 2009 | 19715752 |
| prolonged hospital and community-based listeriosis outbreak caused by ready-to-eat scalded sausages. | listeria monocytogenes is a foodborne bacterial pathogen. immunocompromised patients are at higher risk of developing invasive listeriosis with high fatality rates. after notification of two patients with listeria that had stayed in the same hospital (hospital a) before the onset of infection, we began an investigation to ascertain the extent of the outbreak, identify its source and prevent further infections. we conducted active case finding by contacting hospital a, reviewing medical records a ... | 2009 | 19716628 |
| multiplexed bead-based mesofluidic system for detection of food-borne pathogenic bacteria. | in the present study, a simple and rapid multiplexed bead-based mesofluidic system (bms) was developed for simultaneous detection of food-borne pathogenic bacteria, including staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, salmonella, enterobacter sakazakii, shigella, escherichia coli o157:h7, and campylobacter jejuni. this system is based on utilization of isothiocyanate-modified microbeads that are 250 mum in diameter, which were immobilized with specific amino-modified ... | 2009 | 19717622 |
| inhibition of listeria monocytogenes in cooked ham by virulent bacteriophages and protective cultures. | protective cultures can be used successfully as an additional hurdle together with phages to reduce growth of listeria monocytogenes on sliced cooked ham. addition of phages resulted in a rapid 10-fold reduction of l. monocytogenes. after 14 to 28 days of storage, a 100-fold reduction was observed in samples with phages and protective culture compared to results for samples with phages alone. | 2009 | 19717623 |
| [influence of mutation l,d-carboxypeptidase-coding gene on dynamics of listeria infection]. | to study the progress of infection caused by mutant listeria monocytogenes with deletion of imo0028 gene coding l,d-carboxypeptidase (protein involved in metabolism of peptidoglycan). | 2009 | 19718829 |
| communication and autoinduction in the species listeria monocytogenes: a central role for the agr system. | in order to withstand changes in their environment, bacteria have evolved mechanisms to sense the surrounding environment, integrate these signals and adapt their physiology to thrive under fluctuating conditions. among these mechanisms, the ability of bacteria to exchange information between cells has become a dynamic field of interest for microbiologists over the past four decades. first described by nelson et al.,1 this phenomenon often referred as either cell-cell communication, quorum sensi ... | 2009 | 19721895 |
| effect of acid stress, antibiotic resistance, and heat shock on the resistance of listeria monocytogenes to uv light when suspended in distilled water and fresh brine. | exposure to sublethal processing treatments can stimulate bacterial stress responses. the purpose of this research was to determine whether adaptation to common food processing stresses encountered during the preparation of ready-to-eat foods affects the dose of uv light required to significantly reduce listeria monocytogenes populations in sterile distilled water and a 9% nacl solution, using uridine as a chemical actinometer. l. monocytogenes strains n1-227 (from hot dog batter), n3-031 (from ... | 2009 | 19722394 |
| assay of enterocin as-48 for inhibition of foodborne pathogens in desserts. | enterocin as-48 was tested against staphylococcus aureus, bacillus cereus, and listeria monocytogenes in different kinds of desserts. the highest activity against s. aureus was detected in baker cream. however, in yogurt-type soy-based desserts and in gelatin pudding, as-48 (175 arbitrary units [au]/g) reduced viable cell counts of s. aureus by only 1.5 to 1.8 log units at most. the efficacy of as-48 in puddings greatly depended on inoculum size, and viable s. aureus counts decreased below detec ... | 2009 | 19722396 |
| complete transcriptional profile of an environmental pathogen. | evaluation of: toledo-arana a, dussurget o, nikitas g et al.: the listeria transcriptional landscape from saprophytism to virulence. nature 459 (7249), 950-956 (2009). bacteria adapt to a variety of environmental conditions through changes in gene regulation and gene expression. listeria monocytogenes is an environmental bacterium that exists as a saprophyte but is capable of transitioning into a pathogen upon ingestion by a susceptible host. in this report, toledo-arana et al. present the compl ... | 2009 | 19722832 |
| control of listeria monocytogenes by lauric arginate on frankfurters formulated with or without lactate/diacetate. | listeria monocytogenes (lm) is a food safety concern that can be associated with ready-to-eat (rte) meat and poultry products because of its persistence in the processing environment. listeriosis has a fatality rate of 28% in immuno-compromised individuals. rte meats receive a lethal heat treatment but may become contaminated by lm after this treatment. federal regulators and manufacturers of rte meats are working to find additional ways to control postprocess contamination by lm in rte meats. t ... | 2009 | 19723207 |
| assessment of cell surface properties and adhesion potential of selected probiotic strains. | to evaluate the physicochemical cell surface and adhesive properties of selected probiotic strains for human use. | 2009 | 19725886 |
| suppression of cell-mediated immunity following recognition of phagosome-confined bacteria. | listeria monocytogenes is a facultative intracellular pathogen capable of inducing a robust cell-mediated immune response to sub-lethal infection. the capacity of l. monocytogenes to escape from the phagosome and enter the host cell cytosol is paramount for the induction of long-lived cd8 t cell-mediated protective immunity. here, we show that the impaired t cell response to l. monocytogenes confined within a phagosome is not merely a consequence of inefficient antigen presentation, but is the r ... | 2009 | 19730694 |
| community-acquired acute bacterial meningitis in the elderly in turkey. | this investigation aimed both to delineate the current status of community-acquired acute bacterial meningitis and to produce data on the interrelationships between clinical, laboratory and therapeutic parameters in the elderly. this retrospective cohort study was conducted in 28 turkish institutions in 159 culture-positive patients over the age of 50 years. streptococcus pneumoniae was the most common pathogen (69.2%), followed by listeria monocytogenes (8.8%). for this reason, antilisterial an ... | 2010 | 19732089 |
| [listerial meningitis at the department of infectious diseases, copenhagen university hospital 1983-2006]. | listeria monocytogenes rarely causes meningitis, but when it does the course can be severe and case fatality rates high. the article describes clinical and laboratory findings as well as treatment and outcome among patients treated for listerial meningitis at the department of infectious diseases, rigshospitalet from 1983-2006. | 2009 | 19732509 |
| modelling the influence of the inoculation level on the growth/no growth interface of listeria monocytogenes as a function of ph, aw and acetic acid. | this research is an extension of previous work reported in gysemans et al. [gysemans, k.p.m., bernaerts, k., geeraerd, a.h., vermeulen, a., debevere, j., devlieghere, f., van impe, j.f., 2007. exploring the performance of logistic regression model types on growth/no growth data of listeria monocytogenes. international journal of food microbiology 114, 316-331.] in which the growth/no growth interface of listeria monocytogenes was modelled as a function of water activity (a(w)), ph and undissocia ... | 2009 | 19732986 |
| potential use of supercritical carbon dioxide to decontaminate escherichia coli o157:h7, listeria monocytogenes, and salmonella typhimurium in alfalfa sprouted seeds. | we sought to develop a method of decontaminating alfalfa sprouts of escherichia coli o157:h7, listeria monocytogenes, and salmonella typhimurium without altering the seed germination capability using supercritical carbon dioxide (sc-co(2)). samples were treated with sc-co(2) at 10, 15, or 20 mpa and temperatures of 35, 40, or 45 degrees c for 5, 10, or 15 min. the germination percentage was measured after three days of germination. generally, treating seeds with sc-co(2) at higher pressures, tem ... | 2009 | 19733412 |
| regulation of mannose phosphotransferase system permease and virulence gene expression in listeria monocytogenes by the eii(t)man transporter. | the eii(t)(man) phosphotransferase system (pts) permease encoded by the mpt operon is the principal glucose transporter in listeria monocytogenes. eii(t)(man) participates in glucose-mediated carbon catabolite repression (ccr) and downregulation of virulence gene expression, and it is the receptor for class iia bacteriocins. the regulation of this important protein and its roles in gene control were examined using derivatives of strain egd-e in which the mpt operon or its regulatory genes, manr ... | 2009 | 19734332 |
| study on the microbial status of unseasoned ground turkey meat from an eu producer-a new product with risk potential? | 1. the aim of this study was to point out potential risks associated with a product new to the market in the european union: unseasoned minced turkey meat. 2. on 6 d of sampling, minced turkey meat from a large-scale eu producer was analysed at 4 processing stages. 3. the packaged minced meat was examined during 10 d of storage, once using the legally recommended conditions (<+2 degrees c), and then under simulated consumer handling; one batch was stored for 3 d at +2 degrees c then kept for 45 ... | 2009 | 19735019 |
| pulsed light inactivation of listeria monocytogenes through different plastic films. | the efficacy of decontamination by pulsed light technology through different plastic films has been assayed using listeria monocytogenes scott a as target microorganism. a 12-mum polyethylene film, a 48-mum polyamide/polyethylene/vinyl acetate-based copolymer, and a 60-mum polyamide/polyethylene copolymer were tested. noble agar plates were surface inoculated and wrapped with different films. unwrapped plates were also analyzed as control. fluences of 0.175 and 0.35 j/cm(2) were applied. pulsed ... | 2009 | 19735198 |
| differences in listeria monocytogenes contamination of rural ohio residences with and without livestock. | to determine the contribution of on-site livestock to the environmental contamination of rural households with listeria monocytogenes, a total of 1779 environmental and food samples were collected from 26 ruminant-farm households and 26 rural households in ohio. l. monocytogenes isolates were identified and differentiated using sequence comparisons of the intragenic regions of inlb and inlc. l. monocytogenes was isolated from shoes, 9.6% (20/208); utility gloves, 5.4% (6/111); kitchen sinks, 1.5 ... | 2010 | 19735200 |
| a molecular beacon-based duplex real-time polymerase chain reaction assay for simultaneous detection of escherichia coli o157:h7 and listeria monocytogenes in milk and milk products. | in this study, a real-time polymerase chain reaction assay based on two specific molecular beacons tagged with different reporter dyes was designed and developed for escherichia coli o157:h7 and listeria monocytogenes in such a way that each pathogen could be detected simultaneously in a single tube and differentiated. the duplex assay was developed by targeting the rfb gene of e. coli o157:h7 and the hly gene of l. monocytogenes using the homemade master reaction mix. the detection limit of the ... | 2009 | 19735201 |
| serovar 4b complex predominates among listeria monocytogenes isolates from imported aquatic products in china. | listeria monocytogenes, the causative organism of listeriosis, is primarily transmitted to humans through contaminated food. in this study, we examined 1275 batches of aquatic products imported from 29 countries and found that 36 batches from 8 countries were contaminated by listeria (2.8%), with l. monocytogenes accounting for 2.6% (33/1275) and l. innocua for 0.2% (3/1275). of the 23 selected l. monocytogenes isolates (from the 33 identified), 15 (65.2%) were of serovar 4b complex (4b, 4d, or ... | 2010 | 19735205 |
| studies on the susceptibility of different culture morphotypes of listeria monocytogenes to uptake and survival in human polymorphonuclear leukocytes. | this study demonstrated that atypical virulent filaments of listeria monocytogenes (rough variant type ii and designated fr for this study), isolated from clinical specimens or generated during exposure to pulsed-plasma gas discharge in liquids, were shown to be capable of survival when engulfed by human polymorphonuclear leukocytes (pmnls). factors shown to significantly influence the maximal respiratory burst response in pmnls and survival of different internalized cell or filament forms of l. ... | 2009 | 19735470 |
| microbial contamination of milk and dairy products from restaurants in spain. | this study was carried out to evaluate the microbiological quality of milk and some dairy products, including 95 lots of warm milk, 95 lots of milk conserved at room temperature, and 75 lots of dairy products collected from restaurants in spain. according to the european microbiological criteria (92/46/eec, 93/43/eec, and commission regulation no. 2073/2005), 31% and 35% of the total examined lots exceed the adopted limits of mesophilic aerobic counts and enterobacteriaceae, respectively. this m ... | 2009 | 19737068 |
| specific osmolyte transporters mediate bile tolerance in listeria monocytogenes. | the food-borne pathogenic bacterium listeria monocytogenes has the potential to adapt to an array of suboptimal growth environments encountered within the host. the pathogen is relatively bile tolerant and has the capacity to survive and grow within both the small intestine and the gallbladder in murine models of oral infection. we have previously demonstrated a role for the principal carnitine transport system of l. monocytogenes (opuc) in gastrointestinal survival of the pathogen (r. sleator, ... | 2009 | 19737907 |
| in vitro activities of three new dihydrofolate reductase inhibitors against clinical isolates of gram-positive bacteria. | bal0030543, bal0030544, and bal0030545 are dihydrophthalazine inhibitors with in vitro potency against gram-positive pathogens. the mic(50)s for methicillin (meticillin)-sensitive staphylococcus aureus, methicillin-resistant staphylococcus aureus, hetero-vancomycin-resistant staphylococcus aureus, and vancomycin-resistant staphylococcus aureus (visa) range from 0.015 to 0.25 microg/ml (mic(90)s < or = 0.5 microg/ml). mic(50)s for beta-hemolytic streptococci range from 0.03 to 0.06 microg/ml, mic ... | 2009 | 19738027 |
| role of cybr, a cytohesin binder and regulator, in cd4(+) t-cell function and host immunity. | cytohesin binder and regulator (cybr) is known to regulate leukocyte adhesion and migration. however, its function in t-cells is poorly understood. here, we investigated the role of cybr in cd4(+) t-cell function and host immunity. cybr inhibited p38 phosphorylation following cd4(+) t-cell stimulation. since p38 regulates the expression of t-box expressed in t-cells (t-bet) but not gata binding protein 3 (gata-3) in t-cells, cybr decreased the expression of t-bet and ifn-gamma in cd4(+) t-cells. ... | 2009 | 19744714 |
| effect of nisin (nisaplin) on the growth of listeria monocytogenes in karashi-mentaiko (red-pepper seasoned cod roe). | the influence of nisaplin, which contains 2.5% nisin, on the growth of listeria monocytogenes in karashi-mentaiko (red-pepper seasoned cod roe) was investigated. the mics of nisaplin for l. monocytogenes (10(8) cfu/ml) were measured; seven isolates showed a value of 1,600 microg/ml and one isolate showed a value of 800 microg/ml. all l. monocytogenes isolates had a mic of 800 microg/ml at 10(6) cfu/ml. the number of l. monocytogenes in karashi-mentaiko stored at 4 degrees c was decreased by nisa ... | 2009 | 19745585 |
| p38 mapk-dependent phagocytic encapsulation confers infection tolerance in drosophila. | hosts employ a combination of two distinct yet compatible strategies to defend themselves against parasites: resistance, the ability to limit parasite burden, and tolerance, the ability to limit damage caused by a given parasite burden. animals typically exhibit considerable genetic variation in resistance to a variety of pathogens; however, little is known about whether animals can evolve tolerance. using a bacterial infection model in drosophila, we uncovered a p38 map kinase-mediated mechanis ... | 2009 | 19748466 |
| regulatory mimicry in listeria monocytogenes actin-based motility. | the actin-based motility of the intracellular pathogen listeria monocytogenes relies on acta, a bacterial factor with a structural domain allowing it to mimic the actin nucleation-promoting activity of host cell proteins of the wasp/wave family. here, we used an rnai-based genetic approach in combination with computer-assisted image analysis to investigate the role of host factors in l. monocytogenes cell-to-cell spread. we showed that the host cell serine/threonine kinase ck2 is required for ef ... | 2009 | 19748468 |
| listeria monocytogenes acta-mediated escape from autophagic recognition. | autophagy degrades unnecessary organelles and misfolded protein aggregates, as well as cytoplasm-invading bacteria. nevertheless, the bacteria listeria monocytogenes efficiently escapes autophagy. we show here that recruitment of the arp2/3 complex and ena/vasp, via the bacterial acta protein, to the bacterial surface disguises the bacteria from autophagic recognition, an activity that is independent of the ability to mediate bacterial motility. l. monocytogenes expressing acta mutants that lack ... | 2009 | 19749745 |
| priming and activation of human ovarian and breast cancer-specific cd8+ t cells by polyvalent listeria monocytogenes-based vaccines. | immunotherapeutic vaccine is potentially an effective strategy to combat cancer. essential components of an effective vaccine must include antigens that are processed by the major histocompatibility complex class i pathway, presented by the tumor major histocompatibility complex molecules, and an effective antigen delivery platform that is capable of breaking self-tolerance. in this study, we characterized a set of ovarian cancer-specific t-cell epitopes delivered by live-attenuated recombinant ... | 2009 | 19752748 |
| development of an escherichia coli expressing listeriolysin-o vaccine against wilms tumor gene 1-expressing tumors. | through their ability to induce cytotoxic t-lymphocytes and inhibit foxp3 t-regulatory cells, escherichia coli expressing listeriolysin-o (llo) and a model tumor antigen have been shown to exert strong antitumor activity. the aim of this study is to extend these observations to a self-protein and clinically relevant tumor antigen associated with most types of adult leukemia: wilms tumor gene 1 (wt1). we demonstrate that an e. coli coexpressing llo and wt1 is capable of inducing a strong antitumo ... | 2009 | 19752749 |
| effects of metal compounds with distinct physicochemical properties on iron homeostasis and antibacterial activity in the lungs: chromium and vanadium. | in situ reactions of metal ions or their compounds are important mechanisms by which particles alter lung immune responses. the authors hypothesized that major determinants of the immunomodulatory effect of any metal include its redox behavior/properties, oxidation state, and/or solubility, and that the toxicities arising from differences in physicochemical parameters are manifest, in part, via differential shifts in lung iron (fe) homeostasis. to test the hypotheses, immunomodulatory potentials ... | 2010 | 19757987 |
| efficient oligonucleotide probe selection for pan-genomic tiling arrays. | array comparative genomic hybridization is a fast and cost-effective method for detecting, genotyping, and comparing the genomic sequence of unknown bacterial isolates. this method, as with all microarray applications, requires adequate coverage of probes targeting the regions of interest. an unbiased tiling of probes across the entire length of the genome is the most flexible design approach. however, such a whole-genome tiling requires that the genome sequence is known in advance. for the accu ... | 2009 | 19758451 |
| cellular pharmacokinetics and intracellular activity of torezolid (tr-700): studies with human macrophage (thp-1) and endothelial (huvec) cell lines. | optimal treatment of infections caused by staphylococcus aureus, listeria monocytogenes and legionella pneumophila requires antibiotics with intracellular activity. linezolid accumulates poorly within cells. torezolid (tr-700) is a novel methyltetrazolyl oxazolidinone with potentially different cellular pharmacokinetic properties. our aim was to examine the accumulation and intracellular activities of torezolid in this context. | 2009 | 19759040 |
| duox2-derived reactive oxygen species are effectors of nod2-mediated antibacterial responses. | generation of microbicidal reactive oxygen species (ros) is a pivotal protective component of the innate immune system in many eukaryotes. nod (nucleotide oligomerisation domain containing protein)-like receptors (nlrs) have been implicated as phylogenetically ancient sensors of intracellular pathogens or endogenous danger signals. nod2 recognizes the bacterial cell wall component muramyldipeptide leading to nfkappab and mapk activation via induced proximity signalling through the serine-threoni ... | 2009 | 19759286 |
| effect of bile salts on the dna and membrane integrity of enteric bacteria. | enteric bacteria are able to resist the high concentrations of bile encountered throughout the gastrointestinal tract. here we review the current mechanisms identified in the enteric bacteria salmonella, escherichia coli, bacillus cereus and listeria monocytogenes to resist the dangerous effects of bile. we describe the role of membrane transport systems, and their connection with dna repair pathways, in conferring bile resistance to these enterics. we discuss the findings from recent investigat ... | 2009 | 19762477 |
| control of listeria monocytogenes in turkey deli loaves using organic acids as formulation ingredients. | the growth of listeria monocytogenes in further-processed meat products has become a major concern and an important food safety issue. the meat and poultry industries have incorporated interventions such as organic acids in marinades to inhibit the growth of l. monocytogenes. in this study, organic acids were utilized in the raw product and as a postcook dip to determine their inhibitory effect on the growth of l. monocytogenes in turkey deli loaves. the turkey deli loaves were processed, cooked ... | 2009 | 19762881 |
| listeria monocytogenes attachment to and detachment from stainless steel surfaces in a simulated dairy processing environment. | the presence of pathogens in dairy products is often associated with contamination via bacteria attached to food-processing equipment, especially from areas where cleaning/sanitation is difficult. in this study, the attachment of listeria monocytogenes on stainless steel (ss), followed by detachment and growth in foods, was evaluated under conditions simulating a dairy processing environment. initially, ss coupons were immersed in milk, vanilla custard, and yogurt inoculated with the pathogen (1 ... | 2009 | 19767476 |
| complex phenotypic and genotypic responses of listeria monocytogenes strains exposed to the class iia bacteriocin sakacin p. | sakacin p is a class iia bacteriocin that is active against the food-borne pathogen listeria monocytogenes, and use of this compound as a biopreservative in foods has been suggested. in the present study, we characterized 30 spontaneous sakacin p-resistant mutants of l. monocytogenes obtained after single exposure to sakacin p. the frequency of development of sakacin p resistance for all strains was in the range from 10(-8) to 10(-9). using the 50% inhibitory concentration (ic(50)) of sakacin p, ... | 2009 | 19767478 |
| the bacterial virulence factor inlc perturbs apical cell junctions and promotes cell-to-cell spread of listeria. | several pathogenic bacteria, including listeria monocytogenes, use an f-actin motility process to spread between mammalian cells. actin 'comet tails' propel listeria through the cytoplasm, resulting in bacteria-containing membrane protrusions that are internalized by neighbouring cells. the mechanism by which listeria overcomes cortical tension to generate protrusions is unknown. here, we identify bacterial and host proteins that directly regulate protrusions. we show that efficient spreading be ... | 2009 | 19767742 |
| card9 facilitates microbe-elicited production of reactive oxygen species by regulating the lygdi-rac1 complex. | in response to invading microorganisms, macrophages engage in phagocytosis and rapidly release reactive oxygen species (ros), which serve an important microbicidal function. however, how phagocytosis induces ros production remains largely unknown. card9, a caspase-recruitment domain (card)-containing protein, is important for resistance to fungal and bacterial infection. the mechanism of card9-mediated bacterial clearance is still mostly unknown. here we show that card9 is required for killing i ... | 2009 | 19767757 |
| in silico reconstitution of actin-based symmetry breaking and motility. | eukaryotic cells assemble viscoelastic networks of crosslinked actin filaments to control their shape, mechanical properties, and motility. one important class of actin network is nucleated by the arp2/3 complex and drives both membrane protrusion at the leading edge of motile cells and intracellular motility of pathogens such as listeria monocytogenes. these networks can be reconstituted in vitro from purified components to drive the motility of spherical micron-sized beads. an elastic gel mode ... | 2009 | 19771152 |
| [spontaneous bacterial peritonitis and listeria monocytogenes]. | | 2009 | 19775592 |
| a qualitative study of the australian midwives' approaches to listeria education as a food-related risk during pregnancy. | to explore midwives' perceptions of food-related risks and their approaches to listeria education during pregnancy. | 2011 | 19775781 |
| sting regulates intracellular dna-mediated, type i interferon-dependent innate immunity. | the innate immune system is critical for the early detection of invading pathogens and for initiating cellular host defence countermeasures, which include the production of type i interferon (ifn). however, little is known about how the innate immune system is galvanized to respond to dna-based microbes. here we show that sting (stimulator of interferon genes) is critical for the induction of ifn by non-cpg intracellular dna species produced by various dna pathogens after infection. murine embry ... | 2009 | 19776740 |
| prevalence and level of listeria monocytogenes and other listeria species in selected retail ready-to-eat foods in the united kingdom. | although listeriosis is a rare cause of human disease in the united kingdom, an increase in the number of cases has been observed since 2001, almost exclusively in persons older than 60 years. this increase prompted this study on the microbiological safety of ready-to-eat (rte) foods, which included those types potentially linked to cases of listeriosis. between may 2006 and april 2007, 6,984 rte foods were sampled (2,168 sliced meats, 1,242 hard cheese, 1,088 sandwiches, 878 butter, 725 spreada ... | 2009 | 19777888 |
| safety-based shelf life model for frankfurters based on time to detect listeria monocytogenes with initial inoculum below detection limit. | the growth of listeria monocytogenes inoculated on frankfurters at four inoculum levels (0.1, 0.04, 0.01, and 0.007 cfu/g) was examined at 4, 8, and 12 degrees c until the time l. monocytogenes populations reached a detectable limit of at least 2 cfu/g. a scaled-down assumption was made to simulate a 25-g sample from a 100-lb batch size in a factory setting by using a 0.55-g sample from a 1,000-g batch size in a laboratory. samples of 0.55 g were enriched in pdx-lib selective medium, and presump ... | 2009 | 19777889 |
| effects of mussel processing soils on the adherence of listeria monocytogenes to polypropylene and stainless steel. | a comparative study of adhesion kinetics of l. monocytogenes (strains cect 5873, cect 936, cect 911, and cect 4032, representing serotypes 1/2a, 1/2b, 1/2c, and 4b, respectively) to polypropylene (pp) and stainless steel (ss) under two surface contamination conditions in plants processing cooked mussel was carried out. the conditions were either (i) contamination of clean surfaces with mussel cooking juice carrying l. monocytogenes or (ii) contamination with l. monocytogenes after soiling with m ... | 2009 | 19777890 |
| properties of whey protein-based films containing organic acids and nisin to control listeria monocytogenes. | whey protein isolate and glycerol were mixed to form a matrix to incorporate antimicrobial agents and produce edible films with antimicrobial activity against listeria monocytogenes strains isolated from cheeses. various organic acids were used to decrease ph down to approximately 3. in a preliminary assay without nisin, the effect of each organic acid was evaluated with respect to the rheological properties of the film solutions and the inhibitory and mechanical properties of the films. lactic, ... | 2009 | 19777891 |
| disinfection of iceberg lettuce by titanium dioxide-uv photocatalytic reaction. | securing the physical quality and microbial safety of fresh foods has been a major focus in the food industry. to improve quality and increase the shelf life of fresh produce, disinfection methods have been developed. titanium dioxide (tio2) photocatalytic reactions under uv radiation produce hydroxyl radicals that can be used for disinfection of foodborne pathogenic bacteria. we investigated the effects of tio2-uv photocatalytic disinfection on the shelf life of iceberg lettuce. counts of natur ... | 2009 | 19777894 |
| comparison between the vitek immunodiagnostic assay system and pcr for the detection of pathogenic microorganisms in an experimental dry sausage during its curing process. | the comparison between the vitek immunodiagnostic assay system (vidas) and pcr methods for the detection of the pathogenic microorganisms salmonella typhimurium, listeria monocytogenes, escherichia coli o157:h7, clostridium perfringens, and staphylococcus aureus in salchich6n (a type of spanish fermented dry sausage) was studied. the automated mini-vidas method and the pcr method were used to detect the presence of these microorganisms in 90 samples grouped into six batches (one control batch, a ... | 2009 | 19777902 |
| sensitivity to acetic acid, ability to colonize abiotic surfaces and virulence potential of listeria monocytogenes egd-e after incubation on parsley leaves. | to investigate how the survival of listeria monocytogenes on parsley leaves may affect its ability to sustain process-related harsh conditions and its virulence. | 2010 | 19778346 |
| synergistic antibacterial and anti-inflammatory activity of temporin a and modified temporin b in vivo. | temporins are antimicrobial peptides secreted by the granular glands of the european red frog (rana temporaria). they are 10-14 amino acid long polypeptides active prevalently against gram positive bacteria. this study shows that a synthetic temporin b analogue (tb-yk), acquires the capacity to act in synergism with temporin a and to exert antimicrobial and anti-inflammatory activity in vivo against gram positive and gram negative bacteria. administration of 3.4 mg/kg of temporin a (ta)+1.6 mg/k ... | 2009 | 19784377 |
| therapeutic cancer vaccines in cervical cancer: phase i study of lovaxin-c. | producing effective therapeutic vaccines has proved much more difficult and challenging than developing cancer preventive vaccines. despite huge research in the area of cancer immunology, fda/emea have not approved any type of cancer treatment vaccine so far. more than 99% of cervical cancers have detectable amounts of human papillomavirus (hpv) dna. integration of high-risk hpv into the host cell genome is followed by continual expression of hpv e6 and e7 oncoproteins, making them excellent tar ... | 2009 | 19785060 |
| survival of clinical and food isolates of listeria monocytogenes through simulated gastrointestinal tract conditions. | twenty-seven strains of listeria monocytogenes previously isolated from food (n = 16) and human patients of listeriosis (n = 11) were characterized and compared based on their ability to survive through the simulated gastrointestinal tract conditions. cells were exposed (60 or 120 min) to low ph in the presence of pepsin, to simulate the digestion in the stomach, and subsequently to bile salts to simulate the digestion in the small intestine (60 or 120 min). their survival was shown to be origin ... | 2010 | 19785538 |
| fan stimulates tnf(alpha)-induced gene expression, leukocyte recruitment, and humoral response. | factor associated with neutral sphingomyelinase activation (fan) is an adaptor protein that constitutively binds to tnf-r1. microarray analysis was performed in fibroblasts derived from wild-type or fan knockout mouse embryos to evaluate the role of fan in tnf-induced gene expression. approximately 70% of tnf-induced genes exhibited lower expression levels in fan-deficient than in wild-type fibroblasts. of particular interest, tnf-induced expression of cytokines/chemokines, such as il-6 and cxcl ... | 2009 | 19786552 |
| sodium chloride affects listeria monocytogenes adhesion to polystyrene and stainless steel by regulating flagella expression. | to study the adhesion capability of seven strains of listeria monocytogenes to polystyrene and stainless steel surfaces after cultivation at various nacl concentrations. | 2009 | 19793195 |
| characterization of isolates of listeria monocytogenes from sludge using pulsed-field gel electrophoresis and virulence assays. | to study the diversity and virulence of listeria monocytogenes isolated from sludge. | 2010 | 19796096 |
| transcriptional and post-transcriptional regulation of the gmar antirepressor governs temperature-dependent control of flagellar motility in listeria monocytogenes. | flagellar motility in listeria monocytogenes (lm) is restricted to temperatures below 37 degrees c due to the opposing activities of the mogr transcriptional repressor and the gmar antirepressor. previous studies have suggested that both the degu response regulator and mogr regulate expression of gmar. in this report, we further define the role of degu for gmar production and flagellar motility. we demonstrate that deletion of the receiver domain of degu has no effect on flagellar motility in lm ... | 2009 | 19796338 |
| the ability to enter into an avirulent viable but non-culturable (vbnc) form is widespread among listeria monocytogenes isolates from salmon, patients and environment. | media-based bacteriological testing will fail to detect non-culturable organisms and the risk of consuming viable but non-culturable (vbnc) listeria monocytogenes is unknown. we have here studied whether l. monocytogenes obtained from seafoods, processing environment and clinical cases enter the vbnc state and assessed the virulence of the non-culturable forms of the bacteria. a number of 16 l. monocytogenes strains were starved in microcosm water at 4 degrees c until loss of culturability. meta ... | 2010 | 19796607 |
| neonatal immunization with listeria monocytogenes induces t cells with an adult-like avidity, sensitivity, and tcr-vbeta repertoire, and does not adversely impact the response to boosting. | listeria monocytogenes (lm) holds promise as a neonatal vaccine vehicle. here we show that lm immunized neonatal mice reached maximal ag-specific cd8(+) t cell expansion after only a single immunization, while adults required two doses. ag-specific cd4(+) t cell expansion in both age groups required a boost to reach its peak. neither functional avidity, sensitivity, nor the tcr-vbeta repertoire of the ag-specific t cells differed between mice immunized as neonates or adults. lastly, neonatal imm ... | 2009 | 19796722 |
| superior protective immunity against murine listeriosis by combined vaccination with cpg dna and recombinant salmonella enterica serovar typhimurium. | preexisting antivector immunity can severely compromise the ability of salmonella enterica serovar typhimurium live vaccines to induce protective cd8 t-cell frequencies after type iii secretion system-mediated heterologous protein translocation in orally immunized mice. to circumvent this problem, we injected cpg dna admixed to the immunodominant p60(217-225) peptide from listeria monocytogenes subcutaneously into balb/c mice and coadministered a p60-translocating salmonella strain by the orogas ... | 2009 | 19797070 |
| inactivation of listeria monocytogenes on unpackaged and vacuum-packaged chicken frankfurters using pulsed uv-light. | the effectiveness of pulsed uv-light on the microbial load and quality of unpackaged and vacuum-packaged chicken frankfurters was investigated. samples were inoculated with listeria monocytogenes scott a on the top surfaces, and then treated with pulsed uv-light for 5, 15, 30, 45, and 60 s at 5, 8, and 13 cm distance from the quartz window in a pulsed uv-light chamber. log reductions (cfu/cm(2)) on unpackaged samples were between 0.3 and 1.9 after 5-s treatment at 13 cm and 60-s treatment at 5 c ... | 2009 | 19799670 |
| edible apple film wraps containing plant antimicrobials inactivate foodborne pathogens on meat and poultry products. | apple-based edible films containing plant antimicrobials were evaluated for their activity against pathogenic bacteria on meat and poultry products. salmonella enterica or e. coli o157:h7 (10(7) cfu/g) cultures were surface inoculated on chicken breasts and listeria monocytogenes (10(6) cfu/g) on ham. the inoculated products were then wrapped with edible films containing 3 concentrations (0.5%, 1.5%, and 3%) of cinnamaldehyde or carvacrol. following incubation at either 23 or 4 degrees c for 72 ... | 2009 | 19799671 |
| universal primer-multiplex pcr approach for simultaneous detection of escherichia coli, listeria monocytogenes, and salmonella spp. in food samples. | escherichia coli, listeria monocytogenes, and salmonella spp. are 3 kinds of the most important food-borne human pathogens. traditional microbiological analysis is labor-intensive, time-consuming, and easily contaminated, thus producing false positive signals; it also involves much subjectivity judgments. multiplex-pcr could be applied to detect multiple target organisms simultaneously to save time and labor, but there is always disproportionate amplification resulting from the disparity of diff ... | 2009 | 19799672 |
| microwave oven heating for inactivation of listeria monocytogenes on frankfurters before consumption. | microwave oven heating was evaluated for inactivation of listeria monocytogenes on inoculated and stored frankfurters. frankfurters formulated without/with 1.5% potassium lactate and 0.1% sodium diacetate were inoculated with l. monocytogenes (1.9 +/- 0.2 log cfu/cm(2)), vacuum-packaged, and stored (4 degrees c) to simulate conditions prior to purchase by consumers. at storage days 18, 36, and 54, packages were opened and placed at 7 degrees c, simulating aerobic storage in a household refrigera ... | 2009 | 19799673 |
| comparative analysis of the virulence of invertebrate and mammalian pathogenic bacteria in the oral insect infection model galleria mellonella. | infection of galleria mellonella by feeding a mixture of bacillus thuringiensis spores or vegetative bacteria in association with the toxin cry1c results in high levels of larval mortality. under these conditions the toxin or bacteria have minimal effects on the larva when inoculated separately. in order to evaluate whether g. mellonella can function as an oral infection model for human and entomo-bacterial pathogens, we tested strains of bacillus cereus, bacillus anthracis, enterococcus faecali ... | 2010 | 19800349 |
| [brain abscess due to listeria monocytogenes in adults: six cases and review of the literature]. | listeria monocytogenes is a gram-positive bacillus, with special tropism for the central nervous system (cns). brain abscess caused by listeria has not been extensively studied, although it accounts for 10% of cns infections due to this microorganism. | 2010 | 19800714 |
| cutting edge: importance of il-6 and cooperation between innate and adaptive immune receptors in cellular vaccination with b lymphocytes. | b lymphocytes are a potential alternative to dendritic cell immunotherapy, with the advantages of relative abundance in peripheral blood and the ability to function as apcs. although b cells express multiple receptors that induce costimulatory molecules, b cell vaccine studies have focused primarily on cd40 stimulation. to optimize the potential efficacy of b cell vaccines (bvac), we compared the capacity of differentially stimulated b cells to induce ag-specific cd8(+) t cell responses in vivo. ... | 2009 | 19801508 |
| autophagy in immunity against intracellular bacteria. | autophagy is an innate immune defense mechanism against various intracellular bacterial pathogens, such as salmonella enterica serovar typhimurium (s. typhimurium), listeria monocytogenes and shigella flexneri. s. typhimurium uses type three secretion systems (t3sss) to invade mammalian cells and replicate in salmonella-containing vacuoles (scvs). a small population of intracellular s. typhimurium is targeted by autophagy shortly after infection. evidence suggests that these bacteria are present ... | 2009 | 19802566 |
| dendritic cell cross-priming is essential for immune responses to listeria monocytogenes. | cross-presentation is now recognized as a major mechanism for initiating cd8 t cell responses to virus and tumor antigens in vivo. it provides an elegant mechanism that allows relatively few dendritic cells (dcs) to initiate primary immune responses while avoiding the consumptive nature of pathogenic infection. cd8 t cells play a major role in anti-bacterial immune responses; however, the contribution of cross-presentation for priming cd8 t cell responses to bacteria, in vivo, is not well establ ... | 2009 | 19806187 |
| inactivation of the wall-associated de-n-acetylase (pgda) of listeria monocytogenes results in greater susceptibility of the cells to induced autolysis. | several species of gram-positive bacteria have cell wall peptidoglycan (syn. murein) in which not all of the sugar moieties are n-acetylated. this has recently been shown to be a secondary effect, caused by the action of a peptidoglycan n-acetylglucosamine deacetylase. we have found that the opportunistic pathogen listeria monocytogenes is unusual in having three enzymes with such activity, two of which remain in the cytoplasm. here, we examine the enzyme (pgda) that crosses the cytoplasmic memb ... | 2009 | 19809250 |
| pcr experion automated electrophoresis system to detect listeria monocytogenes in foods. | listeria monocytogenes is frequently found as a contaminant in raw and ready-to-eat foods. the ability of l. monocytogenes to multiply at refrigeration temperatures and to grow in a wide range of ph values is of particular concern for food safety. according to the european union regulation on microbiological criteria for foodstuffs, l. monocytogenes must be absent in some categories of ready-to-eat foods. the standard microbiological method for l. monocytogenes detection in foods (iso 11290-1: 1 ... | 2009 | 19810054 |
| typing of listeria monocytogenes strains isolated in italy by inla gene characterization and evaluation of a new cost-effective approach to antisera selection for serotyping. | in this study, 105 listeria monocytogenes strains isolated from humans, foods and environmental samples were characterized using several typing methods. moreover, serotyping procedure was evaluated, and a cost-effective methodological approach based on preliminary pcrs screening was proposed. | 2010 | 19811568 |
| listeria as an enteroinvasive gastrointestinal pathogen. | the bacterium listeria monocytogenes is the causative agent of listeriosis, a highly fatal opportunistic foodborne infection. listeria spp. are isolated from a diversity of environmental sources, including soil, water, effluents, a large variety of foods, and the feces of humans and animals. recent outbreaks demonstrated that l. monocytogenes can cause gastroenteritis in otherwise healthy individuals and more severe invasive disease in immunocompromised patients. common symptoms include fever, w ... | 2009 | 19812983 |
| listeria monocytogenes ctap is a multifunctional cysteine transport-associated protein required for bacterial pathogenesis. | the bacterial pathogen listeria monocytogenes survives under a myriad of conditions in the outside environment and within the human host where infections can result in severe disease. bacterial life within the host requires the expression of genes with roles in nutrient acquisition as well as the biosynthesis of bacterial products required to support intracellular growth. a gene product identified as the substrate-binding component of a novel oligopeptide transport system (encoded by lmo0135) wa ... | 2009 | 19818015 |
| surveillance for listeria monocytogenes and listeriosis, 1995-2004. | canadian cases and outbreaks of illness caused by listeria monocytogenes between 1995 and 2004 were assessed. isolates (722 total) were characterized by serotyping, and pulsed-field gel electrophoresis (pfge) was performed to provide a means of detecting case clusters. rates of listeriosis remained fairly consistent during the period of study, and patient characteristics were similar to those seen in studies of other populations. most isolates were obtained from blood and cerebrospinal fluid, al ... | 2010 | 19818199 |
| effects of bilimbi (averrhoa bilimbi l.) and tamarind (tamarindus indica l.) juice on listeria monocytogenes scott a and salmonella typhimurium atcc 14028 and the sensory properties of raw shrimps. | the potential of using juice of bilimbi (averrhoa bilimbi l.) and tamarind (tamarindus indica l.) to reduce listeria monocytogenes scott a and salmonella typhimurium atcc 14028 populations on raw shrimps after washing and during storage (4 degrees c) was investigated. the uninoculated raw shrimps and those inoculated with approximately 9 log cfu/ml of l. monocytogenes scott a and s. typhimurium atcc 14028 were washed (dipped or rubbed) in distilled water (sdw) (control), bilimbi or tamarind juic ... | 2009 | 19818521 |
| mast cell-mediated antigen presentation regulates cd8+ t cell effector functions. | the characteristics, importance, and molecular requirements for interactions between mast cells (mcs) and cd8(+) t cells have not been elucidated. here, we demonstrated that mcs induced antigen-specific cd8(+) t cell activation and proliferation. this process required direct cell contact and mhc class i-dependent antigen cross-presentation by mcs and induced the secretion of interleukin-2, interferon-gamma, and macrophage inflammatory protein-1alpha by cd8(+) t cells. mcs regulated antigen-speci ... | 2009 | 19818652 |
| analyzing the power and error of listeria monocytogenes growth challenge studies. | domestic and international food safety policy developments have spurred interest in the design and interpretation of experimental growth challenge studies to determine whether ready-to-eat (rte) foods are able to support growth of listeria monocytogenes. existing challenge study protocols and those under development differ markedly in terms of experimental design and the acceptance criteria under which a rte food is determined not to support l. monocytogenes growth. consequently, the protocols d ... | 2009 | 19822374 |
| mycobacterium avium subsp. paratuberculosis (map) as a modifying factor in crohn's disease. | crohn's disease (cd) is a multifactorial syndrome with genetic and environmental contributions. mycobacterium avium subspecies paratuberculosis (map) has been frequently isolated from mucosal tissues of patients with cd but the cellular immune response to this bacterium has been poorly described. our aim was to examine the influence of map on t-cell proliferation and cytokine responses in patients with inflammatory bowel disease (ibd). | 2010 | 19824071 |
| an enzyme-amplified amperometric dna hybridisation assay using dna immobilised in a carboxymethylated dextran film anchored to a graphite surface. | this report describes a simple methodology for preparation of an enzyme-amplified amperometric dna hybridisation assay using solution-phase ferrocenemethanol mediation of glucose oxidase oxidation of glucose. the recognition layer consists of amine-terminated ssdna (designed for binding of the sequence ssra gene of listeria monocytogenes) bound within a carboxymethylated dextran film that is anchored to a graphite electrode. anchoring sites are provided by electrochemical grafting of an arylamin ... | 2010 | 19828307 |
| fulminant listerial infection of the central nervous system in an otherwise healthy patient: a case report. | the mortality of listerial rhombo-encephalitis exceeds 26% and may involve otherwise healthy patients. a case is presented of a man with fatal listerial infection of the central nervous system that was monitored in an intensive care unit. | 2009 | 19830196 |
| bioactivities of plectranthus ecklonii constituents. | plectranthus ecklonii benth. is traditionally used in south africa for treating stomach aches, nausea, vomiting and meningitis. bioassay-guided fractionation of the ethyl acetate extract of the plant led to the isolation of two known compounds, parvifloron d and parvifloron f, neither of which has been previously reported for this species. the compounds exhibited minimum inhibitory concentrations of 15.6 and 31.2 microg/ml, respectively against listeria monocytogenes, whereas the values against ... | 2009 | 19831023 |
| adherence characteristics of listeria strains isolated from three ready-to-eat meat processing plants. | over 1,560 non-food contact surface swabs and raw meat ingredient samples were collected from three ready-to-eat meat processing plants (520 from each plant) from 1998 to 1999, resulting in the recovery of 259 isolates of listeria obtained from postprocess areas including drains, floors, garbage bins, cart wheels, walls, equipment surfaces, tables, brooms, pallet jacks, hoses, ladders, and waste chutes. we further examined 246 of the 259 isolates for adherence phenotype and used pcr to identify ... | 2009 | 19833036 |
| development and validation of an extensive growth and growth boundary model for listeria monocytogenes in lightly preserved and ready-to-eat shrimp. | an existing cardinal parameter growth and growth boundary model for listeria monocytogenes (o. mejlholm and p. dalgaard, j. food prot. 70:70-84 and 2485-2497, 2007) was expanded with terms for the effects of acetic, benzoic, citric, and sorbic acids to include a total of 12 environmental parameters and their interactive effects. the new model predicted growth rates (micro(max) values) of l. monocytogenes accurately with bias and accuracy factors of 1.0 and 1.5, respectively, for 16 batches of br ... | 2009 | 19833037 |
| dose of uv light required to inactivate listeria monocytogenes in distilled water, fresh brine, and spent brine. | the purpose of this research was to establish the dose of uv light (253.7 nm) needed to inactivate listeria monocytogenes in distilled water, fresh brine (9% nacl), spent brine, and diluted (5, 35, and 55%) spent brine, using uridine as a chemical actinometer. strains n1-227 (isolated from hot dog batter), n3-031 (isolated from turkey franks), and r2-499 (isolated from meat) were mixed in equal proportions and suspended in each solution prepared so as to contain 10(-4) m uridine. samples were ir ... | 2009 | 19833038 |
| development of an oligonucleotide-based microarray to detect multiple foodborne pathogens. | escherichia coli o157:h7, salmonella enterica, listeria monocytogenes and campylobacter jejuni are considered important pathogens causing the most food-related human illnesses worldwide. current methods for pathogen detection have limitations in the effectiveness of identifying multiple foodborne pathogens. in this study, a pathogen detection microarray was developed using various 70-mer oligonucleotides specifically targeting the above pathogens. to reduce the cost of detection, each microarray ... | 2010 | 19833198 |
| isolation and 2-d-dige proteomic analysis of intracellular and extracellular forms of listeria monocytogenes. | the pathogenicity of listeria monocytogenes is related to its ability of invading and multiplying in eukaryotic cells. its main virulence factors are now well characterized, but limited proteomic data is available concerning its adaptation to the intracellular environment. in this study, l. monocytogenes egd (serotype 1/2a) grown in human thp-1 monocytes (24 h) were successfully separated from host organelles and cytosolic proteins by differential and isopycnic centrifugation. for control, we us ... | 2009 | 19834917 |
| [characterization of a naturally atypical listeria monocytogenes strain lacking inlab]. | we attempted to gain insights into the phenotypic and genetic characteristics of a naturally atypical listeria monocytogenes strain s10 lacking inlab. | 2009 | 19835164 |
| differential effects of cytokines and corticosteroids on toll-like receptor 2 expression and activity in human airway epithelia. | the recognition of microbial molecular patterns via toll-like receptors (tlrs) is critical for mucosal defenses. | 2009 | 19835594 |
| comparison of different application strategies of divergicin m35 for inactivation of listeria monocytogenes in cold-smoked wild salmon. | cold-smoked salmon treated with divergicin m35-producing carnobacterium divergens m35, c. divergens atcc 35677 (a non-producer of bacteriocin), purified divergicin m35 or supernatants of c. divergens m35 culture in snow crab hepatopancreas (sch) medium or mrs broth was challenged with listeria monocytogenes (up to 10(3) cfu/g). samples were stored at 4 degrees c for up to four weeks. l. monocytogenes, total bacterial and lactic acid bacterial counts were determined along with changes in total vo ... | 2009 | 19835762 |
| inhibition by lactobacillus sakei of other species in the flora of vacuum packaged raw meats during prolonged storage. | the abilities of five lactobacillus sakei strains and one lactococcus lactis strain to retain inhibitory activity against several target organisms in the flora of product during 12 weeks storage of vacuum-packaged lamb and beef was investigated. l. sakei strains were generally found capable of developing dominant populations on both beef and lamb. l. lactis 75 grew poorly on lamb did not inhibit co-inoculated brochothrix thermosphacta. lamb inoculated with the sakacin-a producer l. sakei lb706 h ... | 2009 | 19835775 |
| resistance of listeria monocytogenes, escherichia coli o157:h7 and campylobacter jejuni after exposure to repetitive cycles of mild bactericidal treatments. | while maintaining nutritional and sensorial attributes of fresh foods mild processing technologies generally deliver microbiologically perishable food products. currently little information exists on possible increase in the resistance of pathogens after repetitive exposure to mild (sub-lethal) treatments. multiple strain-cocktails of listeria monocytogenes, escherichia coli o157:h7 and campylobacter jejuni were exposed to 20 consecutive cycles of sub-lethal inactivation by three different techn ... | 2009 | 19835777 |