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evaluation of the dipstreak, a new device with an original streaking mechanism for detection, counting, and presumptive identification of urinary tract pathogens.dipstreak is a new urine culture device with two types of agar attached back-to-back on a plastic paddle. it combines dip-slide technology and an original streaking inoculation mechanism, allowing for bacterial counting and colony isolation. the performance of the dipstreak device with two different medium formulations, chromagar and macconkey media in study a and uriselect 3 and macconkey media in study b, was evaluated and compared to that of the reference streak method by using plates of cyst ...200212037082
[piperacillin/tazobactam--tazocin. a penicillin-based alternative to 3rd generation cephalosporins and carbapenems].the antibiotic piperacillin/tazobactam has recently been licensed for use in denmark. piperacillin/tazobactam combines a well known beta-lactam antibiotitic, piperacillin, and an inhibitor of bacterial beta-lactamase, tazobactam. the combination of piperacillin and tazobactam compared to piperacillin alone has an expanded antimicrobial spectrum, which includes klebsiellae, escherichia coli, and proteus vulgaris resistant to ampicillin, as well as beta-lactamase-producing staphylococcus aureus. a ...200212043402
a novel ophthalmic presentation of the proteus syndrome.to report a patient with the proteus syndrome who had ocular complications not previously described in the literature.200212045066
[a case of stone formation in the mainz pouch using appendix as the efferent limb: a case report].a large calculus (6.0 x 4.5 x 7.0 cm sized) in the continent urinary pouch was detected incidentally by computed tomography in a 7-year-old man with a gall bladder stone. he underwent a total cystectomy and mainz pouch formation using appendix as the efferent limb for bladder cancer 7 years ago. urine culture showed proteus mirabilis and escherichia coli, and urine ph was 8.5. the stone was removed successfully through a pouchotomy and composed mainly of struvite. the stone analysis was map; 88% ...200212056043
new carbenicillin-hydrolyzing beta-lactamase (carb-7) from vibrio cholerae non-o1, non-o139 strains encoded by the vcr region of the v. cholerae genome.in a previous study, an analysis of 77 ampicillin-nonsusceptible (resistant plus intermediate categories) strains of vibrio cholerae non-o1, non-o139, isolated from aquatic environment and diarrheal stool, showed that all of them produced a beta-lactamase with a pi of 5.4. hybridization or amplification by pcr with a probe for bla(tem) or primers for bla(carb) gene families was negative. in this work, an environmental ampicillin-resistant strain from this sample, me11762, isolated from a waterwa ...200212069969
[yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2000--ii. gram-negative bacteria].the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2000 were yearly evaluated and compared with those of other cephems, oxacephems, and carbapenems. thirty-two species 2,697 strains of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis (n = 125), escherichia coli (n = 250), citrobacter freu ...200212071094
comparative evaluation of two commercial chromogenic media for detection and presumptive identification of urinary tract pathogens.the performance of two commercial chromogenic media for the isolation and presumptive identification of urinary tract pathogens, the cps id2 (biomérieux, france) and the chromagar orientation (bbl becton dickinson, usa), was evaluated and compared with that of cystine-lactose-electrolyte-deficient agar and tryptic soy agar with 5% sheep blood. the detection, determination of bacterial counts, and presumptive identification of bacteria causing urinary tract infections were evaluated in 3,000 urin ...200212072939
numbers and types of microorganisms in vacuum-packed cold-smoked freshwater fish at the retail level.fifty-four packages (each one belonging to a different lot) of vacuum-packed cold-smoked salmon (30) and trout (24) produced by six spanish smokehouses were obtained at retail level after 3 weeks storage at 2+/-1 degrees c. sensorial, chemical, physicochemical and microbiological characteristics were examined. overall, ph, a(w), salt content in water phase, aerobic plate counts at 30 and 25 degrees c. levels of enterobacteriaceae, lactic acid bacteria (lab), fungi and presumptive aeromonads and ...200212076034
proteus syndrome of the hand.proteus syndrome exhibits an expanded phenotype. we report a case with restricted macrodactyly and cerebriform hypertrophy of one palm. the differential diagnosis with macrodactyly and other hamartomatous overgrowth syndromes is discussed.200212077537
principles for the surgical management of patients with proteus syndrome and patients with overgrowth not meeting proteus criteria.proteus syndrome is a rare, sporadic disorder consisting of disproportionate overgrowth of multiple tissues, vascular malformations, and connective tissue or epidermal nevi. patients with proteus syndrome present with diverse and variable phenotypes because of the syndrome's mosaic pattern of distribution.200212077761
[use of sparfloxacin (sparflo) in treating complicated urologic infections].the authors review the effectiveness of a novel fluoroquinolone drug sparfloxacine (sparflo, dr. reddy's laboratories) in the treatment of patients with complicated forms of pyelonephritis and prostatitis. both gramnegative and grampositive agents causing complicated urologic infections were highly sensitive to sparfloxacine: enterobacter spp.--40.8%, pseudomonas aeruginosa--38.5%, proteus spp.--42.6%, e. coli--91.4%, staphylococcus spp.--80.0%, enterococcus faecalis--21.4%. sparfloxacine was us ...200212077817
[experimental study on detection of schistosoma japonicum 5d gene by using pcr].to search for an effective method to diagnose the schistosomiasis and evaluate the effect of treatment.199812078210
synthesis and pharmacological activities of 1,8-naphthyridine derivatives.in the present study, a series of 2-substituted-4-methyl-7-amino/4,7-dimethyl-1,8-naphthyridines were synthesized and characterized by ir, 1h-nmr and elemental analysis. the compounds were investigated for anticonvulsant (125, 250 mg/kg), cardiac and antimicrobial activities. the compounds were screened for antibacterial activity against gram (+) bacteria (staphylococcus epidermidis, bacillus subtilis, enterococcusfaecalis and micrococcus luteus) and gram (-) bacteria (proteus vulgaris, pseudomo ...200212081151
the molecular mechanism of translocation through the nuclear pore complex is highly conserved.in this report we investigated the activity of vertebrate nuclear transport factors in a primitive organism, amoeba proteus, to better understand evolutionary changes in the transport mechanisms of organisms expected to have different requirements for nucleocytoplasmic exchange. it was initially determined that fxfg-containing nucleoporins and ran, both of which are essential for nuclear import in vertebrates, as well as yeast, are also present and functional in amoebae. this suggests that there ...200212082159
bacterial population of a two-phase anaerobic digestion process treating effluent of cassava starch factory.different groups of microorganisms in a two-phase anaerobic system were enumerated to evaluate the prevalence of specific groups and species. total and fecal coliforms showed similar values both in acidogenic and methanogenic reactors. the fecal streptococci were 4-fold higher in the acidogenic reactor, when compared with those of the methanogenic reactor. as expected, no methane forming or sulfate reducing bacteria were found in the acidogenic reactor. the populations of methanogenic bacteria w ...200212088382
emerging fluoroquinolone-resistance for common clinically important gram-negative bacteria in taiwan.the in vitro antimicrobial activity of the three most commonly used fluoroquinolones (norfloxacin, ofloxacin and ciprofloxacin) was measured for 2235 isolates of seven common pathogenic gram-negative bacteria. collected from a major teaching hospital in taiwan during the years 1985-86, 1989-90, and 1996-97, the samples were evaluated using the agar dilution method. the overall susceptibility to fluoroquinolones has decreased rapidly after the wide use of these antimicrobial agents. isolates of e ...200212088622
multidrug resistant neonatal sepsis in peshawar, pakistan.to investigate the spectrum of organisms causing neonatal sepsis in peshawar, pakistan and to assess their sensitivity to various groups of drugs.200212091293
[species composition and microbial contamination of atmospheric humidity condensate and potable water from the mir water supply systems during mir main missions 4 through 27].analyzed were quantitative and qualitative characteristics, and processes of formation and transformation of microflora in the atmospheric humidity condensate (ahc) and potable water (pw) regenerated from the condensate by the long-operating system (srv-k) aboard space station mir. the paper presents data on species composition of microflora of ahc, and pw regenerated by srv-k in the period from 1989 through to 1999. the following microbial species were identified in ahc: alcaligenes (14.8%), ba ...200212098948
survival of enterobacteria in liquid cultures during microwave radiation and conventional heating.bacteria in food have been reported to survive in larger numbers after processing by microwave radiation than after conventional processing. the bactericidal effect of a domestic microwave oven (sharp r-7280) on certain pathogenic enterobacteria species was investigated in vitro, in comparison with conventional heating (boiling). the death rates of different nosocomial strains of escherichia coli, salmonella sofia, salmonella enteritidis, proteus mirabilis and pseudomonas aeruginosa were tested. ...199512099299
a comparison of the performance of cystine lactose electrolyte deficient (cled) agar with oxoid chromogenic urinary tract infection (cuti) medium for the isolation and presumptive identification of organisms from urine.as part of the uk antimicrobial resistance strategy and action plan, the public health laboratory service (phls) is required to collect antibiotic susceptibility data so that resistance trends and patterns can be monitored. most laboratories report urine gram negative isolates, as "coliforms" according to morphological appearance, but without an acceptable identification system the antimicrobial surveillance data will be meaningless. commercially available identification systems tend to be expen ...200212101200
swarming-coupled expression of the proteus mirabilis hpmba haemolysin operon.the hpma haemolysin toxin of proteus mirabilis is encoded by the hpmba locus and its production is upregulated co-ordinately with the synthesis and assembly of flagella during differentiation into hyperflagellated swarm cells. primer extension identified a sigma(70) promoter upstream of hpmb that was upregulated during swarming. northern blotting indicated that this promoter region was also required for concomitant transcription of the immediately distal hpma gene, and that the unstable hpmba tr ...200212101306
morphine, the proteus of organic molecules.this feature article encapsulates the senior author's longstanding interests in opiate chemistry and attempts to place it within an historical context and against the backdrop of related work by others who have viewed morphine as one of the pinnacles of natural product synthesis. biomimetic and 'bioanalogous' routes to the morphine skeleton are discussed followed by approaches based on the elaboration of phenanthrene platforms. the latter include an asymmetric synthesis of ent-morphine developed ...200212109065
epidemiology and genetics of rheumatoid arthritis.the prevalence of rheumatoid arthritis (ra) is relatively constant in many populations, at 0.5-1.0%. however, a high prevalence of ra has been reported in the pima indians (5.3%) and in the chippewa indians (6.8%). in contrast, low occurrences have been reported in populations from china and japan. these data support a genetic role in disease risk. studies have so far shown that the familial recurrence risk in ra is small compared with other autoimmune diseases. the main genetic risk factor of r ...200212110146
the etiology of urinary tract infection: traditional and emerging pathogens.the microbial etiology of urinary infections has been regarded as well established and reasonably consistent. escherichia coli remains the predominant uropathogen (80%) isolated in acute community-acquired uncomplicated infections, followed by staphylococcus saprophyticus (10% to 15%). klebsiella, enterobacter, and proteus species, and enterococci infrequently cause uncomplicated cystitis and pyelonephritis. the pathogens traditionally associated with uti are changing many of their features, par ...200212113867
role of pten, a lipid phosphatase upstream effector of protein kinase b, in epithelial thyroid carcinogenesis.both benign and malignant thyroid disease are well-established components of cowden syndrome (cs), an autosomal dominant disorder characterized by multiple hamartomas and breast cancer that may be considered a phakomatosis. the susceptibility gene for cs is pten, a tumor suppressor gene on 10q23.3 that encodes a lipid phosphatase that lies upstream of protein kinase b (akt). interestingly, carney complex is also a phakomatosis where multiple endocrine neoplasias are prominent and thyroid cancer ...200212119278
emerging antimicrobial resistances among proteus mirabilis in europe: report from the mystic program (1997-2001). meropenem yearly susceptibility test information collection.resistance patterns that are currently problematic in europe can vary greatly within the same species over time, among various patient populations and among geographic regions on the same continent. the results from the meropenem yearly susceptibility test information collection (mystic) program, which monitors carbapenem resistance rates in institutions using meropenem, were used to determine resistance differences among proteus mirabilis. mic results from 688 p. mirabilis strains were classifi ...200212120879
antibiotic resistance of bacteria associated with community-acquired urinary tract infections in the southern area of the gaza strip.the aim of this study was to identify the microorganisms that cause "community-acquired" urinary tract infections among adults and to investigate their resistance to fourteen selected antimicrobial agents. the uropathogens identified in 121 positive midstream urine cultures from the 270 subjects included in this study were escherichia coli (57.9%), proteus species (9.9%), enterobacter species (7.4%), klebsiella species (6.6%), pseudomonas species (5.8%), staphylococcus saprophyticus (5.0%), ente ...200212120880
resistant pathogens in urinary tract infections.antimicrobial susceptibility of bacteria causing urinary tract infection (uti) has evolved over several decades as antimicrobial exposure has repeatedly been followed by emergence of resistance. older populations in the community, long-term care facilities, or acute care facilities have an increased prevalence of resistant bacteria isolated from uti. resistant isolates are more frequent in long-term care populations than the community. resistant isolates include common uropathogens, such as esch ...200212121518
dna gyrase and topoisomerase iv mutations associated with fluoroquinolone resistance in proteus mirabilis.mutations associated with fluoroquinolone resistance in clinical isolates of proteus mirabilis were determined by genetic analysis of the quinolone resistance-determining region (qrdr) of gyra, gyrb, parc, and pare. this study included the p. mirabilis type strain atcc 29906 and 29 clinical isolates with reduced susceptibility (mic, 0.5 to 2 microg/ml) or resistance (mic, > or =4 microg/ml) to ciprofloxacin. susceptibility profiles for ciprofloxacin, clinafloxacin, gatifloxacin, gemifloxacin, le ...200212121936
lomefloxacin versus ciprofloxacin in the treatment of chronic bacterial prostatitis.a total of 182 patients with chronic bacterial prostatitis (cbp) were recruited into this multicentre prospective, randomized clinical study. of these, 93 were treated orally with lomefloxacin (lomx) 400 mg once daily and 89 with ciprofloxacin (cipx) 500 mg twice daily for 4 weeks. at 5-9 days 90/90 vs 86/86, at 4-6 weeks 82/83 vs 82/82, at 3 months 80/79 vs 78/75, and at 6 months 78/75 vs 75/72 patients aging from 18 to 70 years were evaluable bacteriologically/clinically according to a modifie ...200212127707
reactivity to bacterial, fungal, and parasite antigens in patients with lymphedema and elephantiasis.both secondary infections and antifilarial immunity are thought to play roles in the development and progression of lymphedema. to investigate this issue, immune responses to a panel of bacterial, fungal, and parasite antigens were examined for women with lymphedema and elephantiasis (n = 28) and for women with no clinical evidence of lymphatic dysfunction who were either microfilaremic (mf+, n = 23) or microfilaria- and filarial antigen-negative (ag-, n = 24). the prevalence and intensity of de ...200212135288
vaccines for proteus mirabilis in urinary tract infection.proteus mirabilis is a documented cause of urinary tract infection (uti) in the complicated urinary tract. urease-mediated urea hydrolysis is responsible for both virulence of the organism and the ability to cause urolithiasis. a urease-negative mutant of p. mirabilis is unable to initiate stone formation and colonizes the kidney at a significantly lower rate. the considerable pathology caused by p. mirabilis warrants the development of a vaccine. we have initiated the advancement of vaccine stu ...200212135833
aetiology of chronic prostatitis.a total of 388 patients with symptoms of chronic prostatitis and inflammatory findings in expressed prostatic secretion (eps) or in a urine sample collected immediately after prostate massage, were examined over a 2 year period at the outpatient department for urogenital infections, university hospital for infectious diseases 'dr fran mihaljević', zagreb, croatia. the infective aetiology was determined in 276 (71.13%) patients. chlamydia trachomatis was the causative pathogen in 109 patients, tr ...200212135835
strategies for the control of catheter encrustation.two general strategies have been adopted to develop catheter materials that resist encrustaion by bacterial biofilms: (a) the incorporation of antimicrobial agents into the polymers and (b) the production of materials with surface properties which prevent the adherence of bacterial cells. our experience to develop non-adherent surfaces which abstracts design from nature is reported. compounds based on 2-methacryloloxyethylphosphorylcholine co-polymerised with long-chain alkyl methacrylates have ...200212135840
green fluorescent protein urea sensors. uropathogenic proteus mirabilis. 200212136763
identification of mrpi as the sole recombinase that regulates the phase variation of mr/p fimbria, a bladder colonization factor of uropathogenic proteus mirabilis.proteus mirabilis is a common cause of urinary tract infection (uti) in individuals with structural abnormalities or long-term catheterization. the expression of mannose-resistant/proteus-like (mr/p) fimbria is phase variable because of the inversion of a 251 bp dna fragment that carries the promoter for the mrp operon. previous studies have shown that mrpi, which is transcribed divergently from the mrp operon, encodes a recombinase capable of switching the orientation of this invertible element ...200212139630
community-acquired bacterial meningitis in adults: the epidemiology, timing of appropriate antimicrobial therapy, and prognostic factors.between january 1986 and december 1999, 109 adult patients with culture-proven community-acquired bacterial meningitis were identified at kaohsiung chang gung memorial hospital. to compare changes over time, the appearance of disease among our patients was divided into two equal time periods: an earlier time period (1986-1992) and a later time period (1993-1999). in this study, there was a decreasing proportion of community-acquired bacterial meningitis compared with nosocomial bacterial meningi ...200212140104
interaction of the atypical prokaryotic transcription activator flhd2c2 with early promoters of the flagellar gene hierarchy.the transcriptional activator flhd2c2 is the master regulator of bacterial flagellum biogenesis and swarming migration, activating the "early" class ii promoters of the large flagellar gene hierarchy. using primer extensions, band-shift assays, and enzymatic and chemical footprinting, we describe the binding of the flhd2c2 heterotetramer to the promoter regions of four class ii flagella operons, fliaz, flhba and the divergent flgamn and flgbcd(efghij). each of the promoter regions was bound by a ...200212144778
evaluation of a new etest for detecting metallo-beta-lactamases in routine clinical testing.several etest (ab biodisk, solna, sweden) gradient formats were developed for detection of metallo-beta-lactamases based on the reduction of imipenem (ip) or ceftazidime (tz) mics in the presence of edta or 2-mercaptopropionic acid (mpa). the etest metallo-beta-lactamase (etest mbl) strips consisted of a double-sided seven-dilution range of ip or tz (4 to 256 microg/ml) and ip or tz (1 to 64 microg/ml) overlaid with a constant concentration of edta or mpa. the prototype strips were evaluated on ...200212149325
epidemiology and prognosis of bacteremia: a 10-y study in a community hospital.in order to determine the epidemiology and factors influencing the outcome of adult bacteremia in a community hospital, episodes of significant bacteremia were recorded prospectively over a 10-y period (1989-98). the following variables were included: age, sex, etiology, acquisition and source of the bacteremia, risk factors, clinical manifestations, empirical antibiotic treatment and outcome. a total of 798 episodes of bacteremia were recorded (436 in males) and 185 (24%) were hospital-acquired ...200212160171
assessment of peo/ptmo multiblock copolymer/segmented polyurethane blends as coating materials for urinary catheters: in vitro bacterial adhesion and encrustation behavior.the effective long-term use of indwelling urinary catheters has often been hindered by catheter-associated infection and encrustation. in this study, the suitability of poly(ethylene oxide) (peo)-based multiblock copolymer/segmented polyurethane (spu) blends as coating materials for the commercial urinary catheters was assessed by measuring swellability, bacterial adhesion, and encrustation behavior. when exposed to pbs (ph 7.4), the blends absorbed a significant amount of water, which was propo ...200212162332
[bacteriologic examination of gallbladder contents].acute calculous (obstructive) cholecystitis develops as a consequence of cystic obstruction and obstruction of bile flow into choledochus. most often it is a result of impacted gallstones in hartman's pouch or the cystic duct. their direct pressure on gallbladder mucosa causes ischemia, necrosis and ulceration with consequential wall edema and obstructed venous flow. this mechanism is further increasing and spreading the inflammatory process. ulcerations may be that extensive, that mucosa is hig ...200212170867
primary cutaneous alveolar rhabdomyosarcoma of the perineum.primary alveolar rhabdomyosarcoma (rms) involving perineal skin is extremely rare, particularly in the infant age group. we report a case of an alveolar rms in a newborn with abnormal symmetrical perineal overgrowth, causing ambiguous morphologic structure of the genitalia. clinical and imaging studies were suggestive of proteus syndrome with lymphatic malformation. histologic examination of the mass showed cutaneous alveolar rms with areas of embryonal and pleomorphic rms features. multiple sup ...200212171502
[effectiveness of imipenem/cilastatin (tienam, msd) in treating complicated infections in urology].complicated urinary infections tend to eventuate in severe pyoseptic complications--bacteriuria, sepsis. the search for methods of fighting agents of urinary infections goes in the direction of perfection of already existing methods and in the direction of design of novel antibacterial drugs. in the middle 1980s the first carbapenem drug-imipenem--was proposed for urological clinical practice. mechanism of its action as that of the other beta-lactam antibiotics consists in impairment of synthesi ...200212180053
vaginal microbiota in healthy pregnant women and prenatal screening of group b streptococci (gbs).the microbiota of the lower female genital tract was evaluated from vaginal swabs obtained from 623 healthy pregnant women at gestation periods of 35-40 weeks. isolated and identified microorganisms were expressed as percentages of total samples. as expected, lactobacilli made up the dominant vaginal microbiota (70%). enterobacteriaceae, mainly escherichia coli, klebsiella spp. and proteus, were present in 38% of the samples, which might reflect the possible contamination of vaginal tract with r ...200212180785
[experimental study on bacteriostasis of chitosan and sodium hyaluronate].to compare the effect and coverage of bacteriostasis of chitosan and sodium hyaluronate.200212181792
[antibiotic properties of the pseudoalteromonas genus bacteria isolated from the black sea water and molluscs].antagonistic properties of 41 strains of alteromonas-like bacteria isolated from the black sea water and molluscs have been studied. being grown on the rich medium "b" for marine bacteria, 21% of strains have shown high antagonistic activity against phytopathogenic fungi; 6% of strains inhibited the growth of bacillus subtilis, proteus vulgaris and candida albicans. spectrum of antagonistic activity was essentially changed on synthetic "bm" medium with acetate, glutamate, alpha-alanine as a sing ...200212190034
characterization of flagella produced by clinical strains of stenotrophomonas maltophilia.stenotrophomonas maltophilia is an emerging nosocomial pathogen associated with opportunistic infections in patients with cystic fibrosis, cancer, and hiv. adherence of this organism to abiotic surfaces such as medical implants and catheters represents a major risk for hospitalized patients. the adhesive surface factors involved in adherence of these bacteria are largely unknown, and their flagella have not yet been characterized biochemically and antigenically. we purified and characterized the ...200212194767
[evaluation of the rapid lumi 'eiken' using chemiluminescent assay for rapid antimicrobial susceptibility testing].one hundred ninety eight clinical isolates, including enterobacteriaceae (70 strains), pseudomonas aeruginosa (20 strains), acinetobacter baumannii (10 strains), staphylococci (50 strains), enterococci (20 strains), streptococcus pneumoniae (15 strains) and haemophilus influenzae (13 strains) were tested for their antimicrobial susceptibilities by using the rapid lumi 'eiken' (rl). as a reference method, broth microdilution method according to the national committee for clinical laboratory stand ...200212199112
proteus syndrome and sudden cardiac arrest; beware of pulmonary embolism! 200212204468
intravitreal penetration of cefepime after systemic administration to humans.to investigate the penetration of cefepime (a fourth-generation cephalosporin) into the vitreous after single-dose intravenous administration to human subjects.200212207129
a case of purple urine bag syndrome (pubs). 200212212364
pathology of a mouse model of x-linked chronic granulomatous disease.a colony of knockout mice (gene designation cybb tm1) has been maintained at this institution for 5 years. these mice are lacking the b subunit of nadph oxidase and are susceptible to experimental infection with aspergillus fumigatus. the purpose of this study was to document the spontaneous diseases present in these mice which are a murine model of x-linked chronic granulomatous disease and to compare these lesions to those of chronic granulomatous disease in humans. lesions were documented in ...200212213046
first report of a parasitic copepod (pennella balaenopterae) infestation in a pinniped.an infestation by the parasitic copepod pennella balaenopterae was found in a stranded, 8-mo-old, female northern elephant seal (mirounga angustirostris). diagnosis was based on the finding of the cephalothoraxes of 14 adult female copepods from three subcutaneous sites. bacteria cultured from lesion exudate included arcanobacterium phocae, escherichia coli, edwardsiella tarda, an enterococcus sp., and proteus mirabilis. the lesions were drained and irrigated with chlorhexidine, and the seal was ...200212216795
development of an elisa using a recombinant 41 kda partial protein (p45n') for the detection of riemerella anatipestifer infections in ducks.riemerella anatipestifer, a gram-negative bacillus, is the causative agent of duck septicemia, a disease which could incur much economic loss in the duck industry. an indirect enzyme-linked immunosorbent assay (elisa) has been developed to facilitate early detection of r. anatipestifer infection in ducks. the antigen used was a recombinant 41 kda n-terminal fragment (rp45n') of a newly characterized r. anatipestifer potential surface protein, p45, which was expressed in escherichia coli as an n- ...200212220809
chemical constituents and antimicrobial activity of the essential oil of lantana xenica.the aim of this work was to evaluate the chemical composition of lantana xenica essential oil and its antimicrobial activity. the oil from the aerial parts of lantana xenica mold. (verbenacea) was obtained by steam distillation and analyzed by gas chromatography/mass spectrometry. the major constituent of the oil was (e)-caryophyllene (35.2 %), with minor amounts of gamma-cadinene (13.3 %), alpha-pinene (9.3 %), ocimene (9.2 %) and germacrene d (6.6 %). the antimicrobial assays showed that the e ...200212221608
[antimicrobial, echinococcidial and immunostimulating properties of the drug cheblin-sk-1].the antimicrobial properties of the drug cheblin-ck-1 (cck-1) were determined in mice intraabdominally inoculated with proteus mirabilis-4691 in a dose of 140-200 million daily cultured microbial bodies. its comparison agent was ampicillin. cck-1 was found to act as an antibiotic similar to ampicillin in its effects. the antimicrobial activity of cck-1 against staphylococcus aureus and escherichia coli isolated from the contents of echinococcal cysts from patients operated on was also establishe ...200212224264
extended-spectrum beta-lactamases.extended-spectrum beta-lactamases (esbls) were discovered in europe in the early 1980s after widespread use of broad-spectrum antibiotics. they are largely derivatives of 3 progenitor beta-lactamases that confer resistance to ampicillin in gram-negative bacteria and are now carried on plasmids. substitutions at the active enzyme site of these progenitor enzymes at single or multiple amino sites have resulted in altered substrate affinities for esbls. depending on the location of the substitution ...200212226798
[clinical and pathogenetic significance of proteus mirabilis antibodies in uveitis associated with joint lesions in children and adolescents].immunological studies in 95 children with uveitis associated with juvenile chronic arthritis revealed infection with proteus mirabilis in 14.7% cases, mainly in cases with reactive arthritis and juvenile rheumatoid arthritis (jra). a clear-cut relationship between p. mirabilis infection and severity of the disease was detected. in 85% children infected with p. mirabilis uveitis was associated with a rapid decrease of ocular functions and development of complications, one of the most frequent of ...200212226974
pathogenic effects of bacteria isolated from larvae of hylesia metabus crammer (lepidoptera: saturniidae).hylesia metabus larvae are susceptible to several pathogens indigenous to the area in which they are found. some larvae show symptoms characteristic of bacterial infection; they become flaccid and lethargic, and show a marked loss of appetite. we isolated and identified 29 bacterial strains from live, dead and experimentally infected h. metabus larvae, and evaluated their pathogenic activity. the bacteria which caused mortality in the larvae were: pseudomonas aeruginosa (60-93.3%), proteus vulga ...200212234536
antimicrobial and anti-inflammatory activity of folklore: mallotus peltatus leaf extract.since ages mallotus peltatus (geist) muell. arg. var acuminatus (euphorbiaceae) leaf and stem bark is used in folk medicine to cure intestinal ailments and skin infections. in several intestinal ailments, localized inflammation is of common occurrence and hence we have evaluated the antimicrobial as well as anti-inflammatory activity of m. peltatus leaf extract. the crude methanol extract of m. peltatus leaves was found to be active against staphylococcus aureus, staphylococcus saprophyticus, st ...200212242000
use of an electronic nose system for diagnoses of urinary tract infections.the use of volatile production patterns produced by bacterial contaminants in urine samples were examined using electronic nose technology. in two experiments 25 and 45 samples from patients were analysed for specific bacterial contaminants using agar culture techniques and the major uti bacterial species identified. these samples were also analysed by incubation in a volatile generation test tube system for 4-5 h. the volatile production patterns were then analysed using an electronic nose syst ...200212243908
neonatal proteus syndrome? 200212244562
bacteriological study of extracted iucds.previous studies have shown the users of intrauterine devices have a 4.4 times greater risk of pelvic inflammatory disease. this present study intends to categorize the distribution of microbes in cultures from different types of extracted copper intrauterine devices (iucds). bacteriological studies of aerobic and anaerobic organisms were performed on the extracted iucds of 522 iucd users; 480 wearing a multiload cu 250, 15 wearing a copper t, 22 lippes loop and 5 a copper 7. cultures were ne ...198912283073
coping with osteomyelitis.osteomyelitis, or infection of the bones, is a common orthopedic condition in the young in developing countries which causes severe deformity and morbidity. acute osteomyelitis is of hematogenous origin, and in adults it results from infected open fractures or a leg ulcer from staphylococcus aureus (over 80%), or mixed with streptococcus pyogenes, pseudomonas spp, proteus spp, escherichia coli, and salmonella typhi. in a classic case of acute osteomyelitis, a child under 10 presents severe bon ...199212285913
structural requirements of the mrna for intracistronic translation initiation of the enterobacterial infb gene.the gene infb encodes the prokaryotic translation initiation factor if2, a central macromolecular component in the formation of the ribosomal 70s initiation complex. in escherichia coli, infb encodes three forms of if2: if2alpha, if2beta and if2gamma. the expression of if2beta and if2gamma is a tandem translation from intact infb mrna and not merely a translation of post-transcriptionally truncated mrna. the molecular mechanism responsible for the ribosomal recognition of the two intracistronic ...200212296821
[etiopathogenic and diagnostic aspects of postabortion peritonitis].the causes, diagnosis, and treatment of postabortion peritonitis are discussed with reference to 85 cases, analyzing the causal agents and their sensitivity to various antibiotic treatments, and classifying them by frequency and clinical symptoms. the following germs were found in the patients: staphylococcus hemolyticus, esch. coli, pseudomonas, proteus, procyaneus, and enterococcus, which showed different responses to the various antibiotics used. the importance of a correct diagnosis of th ...197512307372
honey: its antibacterial action in the treatment of gastroenteritis.the results of several recent "in vitro" studies using honey in oral rehydration solutions (ors) givien to infants and children with gastroenteritis showed that the use of honey had caused a reduction in the duration of bacterial diarrhea, and it had worked well as a substitute for glucose in an oral rehydration solution containing electrolytes. studies have confirmed that honey shortens the duration of diarrhea in patients with bacterial gastroenteritis through its antibacterial properties. i ...198512314387
[microbiological screening and postoperative course in patients undergoing first trimester abortion].pelvic infections are a serious and widespread gynecological disease, often leading to sterility. procedures like evacuation of the products of conception by vacuum suction are known to facilitate their appearance. 300 patients were examined the same day of the abortion (first trimester); before the hysterosuction a vaginal tampon was taken from the cervix and from the posterior fornix. the patients were invited to come back for an exam in two weeks and to refer to the clinic for any problem ...198512315928
[a comparative study of the various organisms causing salpingitis and their potential presence in iud wearers].a prospective study was undertaken to compare the enbdometrial and vaginal bacteria of a continuous series of 283 patients who had iuds removed between june 1, 1979 and june 1, 1983, by the same clinician. measures were taken to avoid contamination of the iud upon removal by the vaginal bacteria, but as a practical matter it was impossible to avoid all contact. the iuds involved were multiload copper 250 or 375, gyne t and t 200, nova t, and dimelys. the number of positive cultures from the ...198512339972
structure of the o-specific polysaccharide of proteus penneri 103 containing ribitol and 2-aminoethanol phosphates.the o-specific polysaccharide of the lipopolysaccharide of proteus penneri strain 103 was studied using 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, h-detected 1h,(13)c hmqc, 1h, 31p hmqc, and hmbc experiments. it was found that the polysaccharide is built up of oligosaccharide-ribitol phosphate repeating units and thus resembles ribitol teichoic acids of gram-positive bacteria. the following structure of the polysaccharide was established:where etn and rib-ol are ethanolamine a ...200212350322
rapid detection of obesumbacterium proteus from yeast and wort using polymerase chain reaction.to design and evaluate pcr primers for the rapid detection of obesumbacterium proteus.200212358688
proteinases of common pathogenic bacteria degrade and inactivate the antibacterial peptide ll-37.effectors of the innate immune system, the anti-bacterial peptides, have pivotal roles in preventing infection at epithelial surfaces. here we show that proteinases of the significant human pathogens pseudomonas aeruginosa, enterococcus faecalis, proteus mirabilis and streptococcus pyogenes, degrade the antibacterial peptide ll-37. analysis by mass spectrometry of fragments generated by p. aeruginosa elastase in vitro revealed that the initial cleavages occurred at asn-leu and asp-phe, followed ...200212366839
serratia type pore forming toxins.the serratia marcescens hemolysin represents a new type of hemolysin and has been studied in great molecular detail with regard to structure, activation and secretion. it has nothing in common with the pore forming toxins of e. coli type (rtx toxins), the staphylococcus aureus alpha-toxin or the thiol activated toxin of group a beta-hemolytic streptococci (streptolysin o). studies on erythrocytes, eukaryotic cells and artificial black lipid membranes, have shown that the mechanism of pore format ...200012369921
revisiting proteus: do minor changes in lectin structure matter in biological activity? lessons from and potential biotechnological uses of the diocleinae subtribe lectins.significant differences in function have been observed among lectins structurally similar to concanavalin a, but their high homology with this widely used lectin has kept them in obscurity. the observation of large differences in the potency of many of these diocleinae lectins as stimulators of interferon-g production by human peripheral blood mononuclear cells has lead to a major effort to unravel their chemical structure and biological activity. modeling studies of some of these lectins reveal ...200112370020
[use of mpo chromogenic culture medium for routine processing of urine cultures].the chromogenic culture medium, mpo, was compared to culture on cled (cystein, lactose, electrolyte-deficient) agar for the detection, enumeration and identification of urinary tract pathogens.200212372235
venous sinus thrombosis after proteus vulgaris meningitis and concomitant clostridium abscess formation.a 19-y-old woman presented with proteus vulgaris meningitis as a complication of chronic otitis media. despite treatment with ceftazidime and amikacin no clinical improvement was observed. cranial mri revealed right-sided mastoiditis/otitis media and venous sinus thrombosis. after mastoidectomy, repeat cranial mri demonstrated abscess formation in the venous sinuses. the abscess was drained. clostridium spp. was isolated from the abscess culture.200212374367
outcome of percutaneous nephrostomy for the management of pyonephrosis.the aim of this study was to evaluate the efficacy of percutaneous nephrostomy (pcn) drainage for the interim management of pyonephrosis.200212376218
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2000). i. susceptibility distribution].the bacterial strains isolated from patients diagnosed as having urinary tract infections (utis) in 10 institutions in japan were supplied between the period of august 2000 and july 2001. then, the susceptibilities of them to many kinds of antimicrobial agents were investigated. the number of them were 511 strains. the breakdown of these strains was gram-positive bacteria as 29.0% and gram-negative bacteria as 71.0%. susceptibilities of these bacteria to antimicrobial agents were as follows; van ...200212378869
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2000). ii. background of patients].five-hundred eighty eight bacterial strains isolated from 435 patients diagnosed as having urinary tract infections (utis) in 10 institutions in japan were supplied between august 2000 and july 2001. then, the clinical background of patients were investigated such as sex, age, and type of infections, infections and kind of bacteria, frequency of bacteria isolation by age and infections, bacteria and infections by timing of antibiotics administration, and bacteria and infections by surgical proce ...200212378870
[in vitro and in vivo antibacterial activities of pazufloxacin mesilate, a new injectable quinolone].we investigated the in vitro and in vivo antibacterial activities of pazufloxacin mesilate (pzfx mesilate), a new injectable quinolone, and obtained the following results. 1) the mic50 and mic90 values of pzfx against clinically isolated gram-positive and -negative bacteria, ranged from 0.0125 to 12.5 micrograms/ml and 0.025 to 100 micrograms/ml, respectively. pzfx showed broad spectrum activity. the antibacterial activities of pzfx against quinolone-susceptible, methicillin-resistant staphyloco ...200212378871
infectious background of patients with a history of acute anterior uveitis.to study the infectious background of patients with a history of acute anterior uveitis (aau) and healthy control subjects.200212379526
multiple antimicrobial resistance in gram negative bacilli isolated from clinical specimens, jimma hospital, southwest ethiopia.a total of 413 clinical specimens (pus, blood, urine, and stool) collected from patients admitted to jimma hospital were cultured for isolation and identification of aerobic bacteria and antimicrobial susceptibility testing. out of these, 124 specimens yielded one or more gram-negative bacilli strains. frequent gram negative bacterial isolates were proteus species 34(27%), klebsiella species 26(21%), enterobacter species 24(19%), and e. coli 24(19%). antimicrobial susceptibility test results sho ...200112380230
identification of a chromosome-borne expanded-spectrum class a beta-lactamase from erwinia persicina.from whole-cell dna of an enterobacterial erwinia persicina reference strain that displayed a penicillinase-related antibiotic-resistant phenotype, a beta-lactamase gene was cloned and expressed in escherichia coli. it encoded a clavulanic-acid-inhibited ambler class a beta-lactamase, erp-1, with a pi value of 8.1 and a relative molecular mass of ca. 28 kda. erp-1 shared 45 to 50% amino acid identity with the most closely related enzymes, the chromosomally encoded enzymes from citrobacter koseri ...200212384342
is there a need for postoperative surveillance after day case groin surgery in children?there is increasing awareness of the value of day case paediatric surgery in fulfilling the needs of the community. is this cost effective, are there hidden complications and is there a need for routine follow-up? seven hundred and thirty-one children attending the red cross war memorial children's hospital day surgery unit over a 32-month period, and undergoing 912 procedures confined to the inguinoscrotal region, were enrolled in the study. surgical procedures performed included inguinal herni ...200212387217
comparison of three chromogenic agar plates for isolation and identification of urinary tract pathogens.to comparatively assess the performance of three chromogenic agar plates, cps id2, chromogenic uti, and usa, for the detection and enumeration of all urinary tract pathogens and the direct identification of escherichia coli, proteus mirabilis and enterococcus spp.200212390282
[psoas abscess. etiology and treatment].psoas abscess is in general a syndrome with low incidence. in the following, two cases are presented which describe the etiology, diagnosis, and treatment of primary and secondary retroperitoneal manifestations. the article mainly focuses on the different respective surgical procedures. because of its rareness, an overview of further cases discussed in the literature is provided.200212395166
characterisation of local isolates of enterobacteriaceae from turkey.20 local isolates of enterics belonging to the genera salmonella, enterobacter, proteus, citrobacter from human, chicken and/or egg were characterised for their antibiotic resistance patterns, plasmid profiles, phage types, outer membrane proteins, and lipopolysaccharide patterns. relatedness of these characteristics for epidemiological analysis was assessed. 18 (90%) strains were resistant to at least one antibiotic and those (multi-drug resistant ones) resisting to two or more antibiotics cons ...200212398294
infestation of fish-culturing communities with fish-borne bacteria: the ghanaian case.twenty different bacterial species were isolated from fishermen and members of communities associated with seven fish ponds with different fertilizer treatments, and an open system. the number and types of the bacterial species were different for the different ponds. generally, neither the geographical location of the ponds nor the type of fertilizer used could be related to the bacterial flora. escherichia coli was the predominant species in all the communities, while escherichia coli, klebsiel ...200212400540
serum levels of prophylactic cefazolin during cardiopulmonary bypass surgery.controversy exists regarding the appropriate prophylactic dose of cefazolin for coronary artery bypass grafting (cabg) surgery requiring cardiopulmonary bypass (cpb) because the effect of cpb on serum drug levels is poorly understood. current standards of prophylaxis are based primarily on empiric studies. few studies have attempted to quantify serum cefazolin levels in either cardiac or noncardiac surgeries. this study was conducted to measure and assess the adequacy of the intraoperative serum ...200212400766
[triclosan, a topical dermatologic agent. in vitro- and in vivo studies on the effectiveness of a new preparation in the new german formulary].triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether) is an antiseptic suitable for formulation as a w/o emulsion. the objective of the present study was to explore its potential utility in atopic dermatitis and prophylactic skin care following leg eczema and leg ulcer treatment.200212402134
enzyme fingerprints by fluorogenic and chromogenic substrate arrays we would like to thank prof. r. furstoss and dr. a. archelas at the faculté des sciences de luminy, marseille, france, for providing a sample of aspergillus niger eh, and dr. c. weijers at the department of food technology and nutrition sciences, wageningen university, the netherlands, for providing a sample of rhodotorula glutinis eh. this work was supported by the university of bern, the european cost program (action d12), the swiss office fédéral de l'education et de la science, protéus sa (nîmes, france), the ministero della università e della ricerca scientifica e tecnologica (murst), and consiglio nazionale delle ricerche (cnr) (roma). p.c. gratefully acknowledges merck for generous financial support from a 2000 adp chemistry award. 200112404444
antimicrobial activity of dequalinium chloride against leading germs of vaginal infections.dequalinium chloride (cas 522-51-0) and povidone iodine (cas 25655-41-8) are known as antiseptic agents and used in the local treatment of vaginal infections. clotrimazole (cas 23593-75-1) is an anti-fungal drug and applied primarily in the therapy of vulvo-vaginal candidiasis and to a lesser extent in bacterial vaginosis and trichomoniasis. however, antimicrobial activities of those three agents in comparison to each other have not been reported so far. to address this issue the antimicrobial a ...200212404886
anti-salmonella activity of alkyl gallates.a series of alkyl gallates (3,4,5-trihydroxybenzoates) was synthesized and tested for their antibacterial activity against salmonella choleraesuis. nonyl (c(9)) and octyl (c(8)) gallates were noted to be the most effective against this food-borne bacterium, each with a minimum bactericidal concentration (mbc) of 12.5 microg/ml, followed by decyl (c(10)) gallate, with a mbc of 25 microg/ml. dodecyl (c(12)) gallate exhibited activity against s. choleraesuis, with a mbc of 50 microg/ml. propyl (c(3 ...200212405763
characterization of cmy-type beta-lactamases in clinical strains of proteus mirabilis and klebsiella pneumoniae isolated in four hospitals in the paris area.we isolated five clinical strains (three proteus mirabilis and two klebsiella pneumoniae) with beta-lactam resistance phenotypes consistent with production of an ampc-type beta-lactamase. the predicted amino acid sequences of the enzymes were typical of class c beta-lactamases. the enzymes were identified as cmy-2, cmy-4 and a new cmy-variant beta-lactamase, cmy-12. the ampc beta-lactamases from the two k. pneumoniae isolates were found to be encoded on self-transferable plasmids. the genes enco ...200212407124
modification of dienes mutual inhibition test for epidemiological characterization of pseudomonas aeruginosa isolates.pseudomonas aeruginosa is an important cause of community-associated and nosocomial infections related to exposure to aqueous environments. such infections often occur in the setting of a common-source outbreak, in which case epidemiological characterization of isolates may be necessary. in this preliminary study, a modification of the dienes mutual inhibition test, ordinarily used to assess the relatedness of swarming proteus mirabilis strains, was used to study 15 p. aeruginosa isolates, with ...200212409411
[fibrin-collagen substance as a vehicle for local application of antibacterial agents].inhibitory activity of fibrin-collagen substance (fcs) impregnated by various antibacterial agents against various microorganisms was investigated. it was demonstrated that antibacterials bind well with fcs, providing its sufficient antibacterial activity. determination of growth-inhibition zones showed that antibacterial agents diffuse into medium well and demonstrated the same activity as discs with antibiotics. for clinical practice it is recommended to use for impregnation the following drug ...200212422648
antimicrobial properties and phytochemical constituents of the leaves of african mistletoe (tapinanthus dodoneifolius (dc) danser) (loranthaceae): an ethnomedicinal plant of hausaland, northern nigeria.african mistletoe (tapinanthus dodoneifolius (dc) danser) called 'kauchi' in hausa is a hemi-plant parasite used ethnomedicinally by the hausa and the fulani tribes of northern nigeria as a remedy for several human and animal ailments that include stomach ache, diarrhoea, dysentery, wound and cancer. screening of the plant, obtained from 14 different hosts, revealed a wide spectrum of antimicrobial activities against certain multiple drug resistant bacterial and fungal isolates of farm animals. ...200212426091
indigoferabietone, a novel abietane diterpenoid from indigofera longeracemosa with potential antituberculous and antibacterial activity.from the stems of indigofera longeracemosa, a novel abietane diterpenoid, indigoferabietone was isolated. the structure was established by spectral techniques. the inhibitory activity of indigoferabietone (1) tested against mycobacterium tuberculosis and candida albicans, and the antibacterial activity tested against staphylococcus aureus, proteus vulgaris, and escherichia coli were found to be significant.200212426956
the inner-core lipopolysaccharide biosynthetic waae gene: function and genetic distribution among some enterobacteriaceae.to determine the function of the waae gene in the biosynthesis of the inner-core lps of klebsiella pneumoniae, a waae non-polar mutant has been constructed. data obtained from the comparative chemical analysis of lps samples obtained from the wild-type, the mutant strain and the complemented mutant demonstrated that the waae gene is involved in substitution of alpha-l-glycero-d-manno-heptopyranose i (l,d-heppi) at the o-4 position by a beta-d-glucopyranose (beta-d-glcp) residue. in addition, dna ...200212427940
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