| current trends in follow-up of trophoblastic function in ruminant species. | during the pregnancy of ruminants, different hormones and proteins are secreted by placenta or corpus luteum allowing the follow up of gestation. among them, progesterone (p4) and pregnancy-associated glycoproteins (pag) were proposed as laboratory tools to establish or to confirm pregnancy diagnosis. in last years, pag assay also provided useful information for researchers working in programs focused on the follow up of trophoblastic function. concentrations of pag appeared as altered after the ... | 2008 | 19261972 |
| searching for the ideal cancer vaccine. | | 2009 | 19263513 |
| cxcr4 is a key regulator of neutrophil release from the bone marrow under basal and stress granulopoiesis conditions. | the number of neutrophils in the blood is tightly regulated to ensure adequate protection against microbial pathogens while minimizing damage to host tissue. neutrophil homeostasis in the blood is achieved through a balance of neutrophil production, release from the bone marrow, and clearance from the circulation. accumulating evidence suggests that signaling by cxcl12, through its major receptor cxcr4, plays a key role in maintaining neutrophil homeostasis. herein, we generated mice with a myel ... | 2009 | 19264920 |
| toll-like receptor activity in recurrent aphthous ulceration. | toll-like receptors (tlr) are membrane proteins that recognize conserved molecules derived from bacterial, virus, fungal or host tissues. activation of tlrs causes the production of cytokines that mediate inflammatory responses and drive t helper (th) 1 and 2 cell development. as an exaggerated th1 immune response is supposed to be involved in pathogenesis of recurrent aphthous ulceration (rau), we suggest that rau patients may have an imbalance in tlr pathways. | 2009 | 19267841 |
| rapid detection of listeria monocytogenes in food by polymerase chain reaction. | the standard conventional methods for the detection of listeria monocytogenes in foods require high time 7 to 10 days to give ready results. to dissolve this problem we have evaluate a short method using polymerase chain reaction (pcr) to analyze food samples. in parallel with this study, a comparison was made between pcr amplification from templates directly prepared from food and the official standard iso procedure 11290-1. in this study we have used a half frazer broth as an enrichment medium ... | 2009 | 19267993 |
| [listeria monocytogenes meningitis in children in france]. | listeriosis is a serious invasive disease which affects mainly pregnant women, newborns and immunocompromised adults. | 2008 | 19268247 |
| behavior of escherichia coli o157:h7, listeria monocytogenes, and salmonella typhimurium in teewurst, a raw spreadable sausage. | the fate of listeria monocytogenes, salmonella typhimurium, or escherichia coli o157:h7 were separately monitored both in and on teewurst, a traditional raw and spreadable sausage of germanic origin. multi-strain cocktails of each pathogen (ca. 5.0 log cfu/g) were used to separately inoculate teewurst that was subsequently stored at 1.5, 4, 10, and 21 degrees c. when inoculated into commercially-prepared batter just prior to stuffing, in general, the higher the storage temperature, the greater t ... | 2009 | 19269050 |
| the probability of growth of listeria monocytogenes in cooked salmon and tryptic soy broth as affected by salt, smoke compound, and storage temperature. | the objectives of this study were to examine and model the probability of growth of listeria monocytogenes in cooked salmon containing salt and smoke (phenol) compound and stored at various temperatures. a growth probability model was developed, and the model was compared to a model developed from tryptic soy broth (tsb) to assess the possibility of using tsb as a substitute for salmon. a 6-strain mixture of l. monocytogenes was inoculated into minced cooked salmon and tsb containing 0-10% nacl ... | 2009 | 19269565 |
| a filtration-based real-time pcr method for the quantitative detection of viable salmonella enterica and listeria monocytogenes in food samples. | we developed a novel filtration-based method that can eliminate dead or severely damaged salmonella enterica and listeria monocytogenes in food samples. this new method can recover all viable bacteria in less than 30 min, and can be coupled with a subsequent bacterial dna extraction and real-time pcr. no statically significant differences (p<0.01) were found between real-time pcr results obtained separately from s. enterica and l. monocytogenes when different ratios of living and dead cells were ... | 2009 | 19269574 |
| [tasks and duties of veterinary reference laboratories for food borne zoonoses]. | reference laboratories are of central importance for consumer protection. field expertise and high scientific competence are basic requirements for the nomination of a national reference laboratory. to ensure a common approach in the analysis of zoonotic hazards, standards have been developed by the reference laboratories together with national official laboratories on the basis of art. 33 of directive (eg) no. 882/2004. reference laboratories function as arbitrative boards in the case of ambiva ... | 2009 | 19271255 |
| cx3cr1+ cd115+ cd135+ common macrophage/dc precursors and the role of cx3cr1 in their response to inflammation. | cx(3)cr1 expression is associated with the commitment of csf-1r(+) myeloid precursors to the macrophage/dendritic cell (dc) lineage. however, the relationship of the csf-1r(+) cx(3)cr1(+) macrophage/dc precursor (mdp) with other dc precursors and the role of cx(3)cr1 in macrophage and dc development remain unclear. we show that mdps give rise to conventional dcs (cdcs), plasmacytoid dcs (pdcs), and monocytes, including gr1(+) inflammatory monocytes that differentiate into tipdcs during infection ... | 2009 | 19273628 |
| listeria monocytogenes in two different poultry facilities: manual and automatic evisceration. | listeriosis is a serious foodborne disease caused by listeria monocytogenes, a pathogen often found in food processing plants. poultry meat and its derivatives may harbor l. monocytogenes even if good manufacturing practices are implanted in abattoirs. little information exists in brazil on the frequency of l. monocytogenes contamination, even though the country is considered the top poultry meat exporter in the world. this study attempted to compare 2 exporters poultry facilities following same ... | 2009 | 19276422 |
| growth inhibition of pathogenic bacteria and some yeasts by selected essential oils and survival of l. monocytogenes and c. albicans in apple-carrot juice. | food safety is a fundamental concern of both consumers and the food industry. the increasing incidence of foodborne diseases increases the demand of using antimicrobials in foods. spices and plants are rich in essential oils and show inhibition activity against microorganisms, which are composed of many compounds. in this research, effects of garlic, bay, black pepper, origanum, orange, thyme, tea tree, mint, clove, and cumin essential oils on listeria monocytogenes aufe 39237, escherichia coli ... | 2009 | 19278342 |
| lmo0038 is involved in acid and heat stress responses and specific for listeria monocytogenes lineages i and ii, and listeria ivanovii. | the genus listeria comprises two pathogenic species, l. monocytogenes and l. ivanovii, as well as four nonpathogenic species, l. innocua, l. weishimeri, l. seeligeri, and l. grayi. within l. monocytogenes, lineages i and ii are responsible for most listeriosis cases, while lineage iii strains are rarely associated with human morbidity but providing important clues for listeria evolution. the gene lmo0038, belonging to the peptidylarginine deiminase family, was involved in the optimal growth unde ... | 2009 | 19278345 |
| outbreak of listeria monocytogenes in an oncology unit associated with sandwiches consumed in hospital. | in may 2003, two adult patients in an oncology unit were diagnosed with listeriosis, and sandwiches consumed in the hospital were identified as a common risk factor. both patients were infected by the same strain of listeria monocytogenes. sandwiches collected from the hospital and external sandwich producer, as well as sites within the manufacturing environment, were contaminated by the same strain of l. monocytogenes. sandwiches consumed in other hospitals have been associated with small clust ... | 2009 | 19278752 |
| epidemiology of listeriosis in austria. | a total of 150 human cases of listeriosis (case definition based on isolation of listeria monocytogenes from normally sterile material) were reported in austria between 1997 and 2007. of these, 14 cases (9.3%) were pregnancy-associated (mother/child illness considered as a single case) with a mean age of 29.3 years (median: 26.5; range 24-36). among the non-pregnancy-associated cases (n = 136), 75 were male (55.2%) and 61 female (44.9%); patients in this group had a mean age of 64.3 years (media ... | 2009 | 19280136 |
| an outbreak of febrile gastroenteritis associated with jellied pork contaminated with listeria monocytogenes. | in september 2008, the austrian agency for health and food safety (ages) learned of an outbreak of diarrheal illness that included a 71-year-old patient hospitalized for gastroenteritis with a blood culture positive for listeria monocytogenes. three stool specimens provided by seven of 19 persons attending a day trip to a foreign city, including a final break at an austrian tavern, yielded l. monocytogenes. all isolates were of serovar 4b and had fingerprints indistinguishable from each other. a ... | 2009 | 19280142 |
| listeria monocytogenes phosphatidylinositol-specific phospholipase c: kinetic activation and homing in on different interfaces. | the phosphatidylinositol-specific phospholipase c (pi-plc) from listeria monocytogenes forms aggregates with anionic lipids leading to low activity. the specific activity of the enzyme can be enhanced by dilution of the protein or by addition of both zwitterionic and neutral amphiphiles (e.g., diheptanoylphosphatidylcholine or triton x-100) or 0.1-0.2 m inorganic salts. activation by amphiphiles occurs with both micellar (phosphatidylinositol dispersed in detergents) and monomeric [dibutroylphos ... | 2009 | 19281241 |
| community-acquired bacterial meningitis in elderly patients: experience over 30 years. | clinical characteristics, etiologies, evolution, and prognostic factors of community-acquired bacterial meningitis in elderly patients are not well known. to improve this knowledge, all episodes of community-acquired bacterial meningitis were prospectively recorded and cases occurring in patients >or=65 years old were selected. during the period 1977-2006, 675 episodes in adults (aged >or=18 yr) were recorded, with 185 (27%) in patients aged >or=65 years old; 76 were male and 109 were female, wi ... | 2009 | 19282702 |
| the solution structure of pgolemi, a high affinity mena evh1 binding miniature protein, suggests explanations for paralog-specific binding to ena/vasp homology (evh) 1 domains. | ena/vasp homology 1 (evh1) domains are polyproline binding domains that are present in a wide range of adaptor proteins, among them ena/vasp proteins involved in actin remodeling and axonal guidance. the interaction of acta, a transmembrane protein from the food-borne pathogen listeria monocytogenes, with evh1 domains has been shown to be crucial for recruitment of the host's actin skeleton and, as a consequence, for the infectivity of this bacterium. we present the structure of a synthetic high ... | 2009 | 19284291 |
| infection and stillbirth. | infection may cause stillbirth by several mechanisms, including direct infection, placental damage, and severe maternal illness. various organisms have been associated with stillbirth, including many bacteria, viruses, and protozoa. in developed countries, between 10% and 25% of stillbirths may be caused by an infection, whereas in developing countries, which have much higher stillbirth rates, the contribution of infection is much greater. in developed countries, ascending bacterial infection, b ... | 2009 | 19285457 |
| abl tyrosine kinases in t-cell signaling. | stimulation of the t-cell antigen receptor (tcr) leads to the activation of signaling pathways that are essential for t-cell development and the response of mature t cells to antigens. the tcr has no intrinsic catalytic activity, but tcr engagement results in tyrosine phosphorylation of downstream targets by non-receptor tyrosine kinases. three families of tyrosine kinases have long been recognized to play critical roles in tcr-dependent signaling. they are the src, zeta-associated protein of 70 ... | 2009 | 19290927 |
| in vitro synergistic effect of gentamicin with the anti-inflammatory agent diclofenac against listeria monocytogenes. | a total of nine listeria monocytogenes strains (seven serotypes) were studied to ascertain whether the non-steroidal anti-inflammatory drug diclofenac (dc) used in combination with the conventional antilisterial antibiotic gentamicin (gm) or ampicillin (am) synergistically augments the efficacy of the antibiotic in vitro. | 2009 | 19291208 |
| the impact of growth history and flagellation on the adhesion of various listeria monocytogenes strains to polystyrene. | the contribution of growth history and flagella to adhesion of listeria monocytogenes was analysed. an in-frame deletion on the flagellin encoding gene (flaa) was performed in l. monocytogenes egd-e to compare its adhesion ability with the parental strain, after cultivation at various ph values and temperatures. the ph, as well as the temperature, affected the adhesion of l. monocytogenes egd-e. in addition, the adhesion of l. monocytogenes egd-e was reduced in energy-depressed cells. conversely ... | 2009 | 19295651 |
| cytoskeleton rearrangements during listeria infection: clathrin and septins as new players in the game. | the study of an infection process can reveal how microbes exploit the host, and can illuminate unknown host cellular functions. invasive pathogens have evolved efficient strategies to promote their internalization within normally non-phagocytic host cells. the so-called "zippering" bacteria present to host cell receptors molecules that mimic endogenous ligands, thereby inducing specific intracellular signaling cascades ultimately resulting in actin polymerization and uptake. here we review how t ... | 2009 | 19296488 |
| [pleural and ascitic fluid infection by listeria monocytogenes. report of one case]. | ascitic and pleural fluids infection by listeria monocytogenes is uncommon. the association of spontaneous bacterial peritonitis and empyema caused by this microorganism has been seldom reported. a 61-year-old male with an alcoholic cirrhosis and an upper right lobectomy for a lung cancer, consulted because of an exacerbation of dyspnea, abdominal pain and fever. listeria-monocytogenes was isolated from ascitic and pleural fluids and from blood cultures. he was successfully treated with ampicill ... | 2008 | 19301777 |
| characterization and purification of a new bacteriocin with a broad inhibitory spectrum produced by lactobacillus plantarum lp 31 strain isolated from dry-fermented sausage. | characterization and purification of a new bacteriocin produced by lactobacillus plantarum lp 31 strain, isolated from argentinian dry-fermented sausage. | 2009 | 19302495 |
| in vitro potency of doripenem tested against an international collection of rarely isolated bacterial pathogens. | doripenem, a new 1beta-methyl parenteral carbapenem, has very broad-spectrum activity against gram-positive and gram-negative aerobic bacteria. as noted here, the spectrum and potency extended to many rarely isolated species sampled by the doripenem global surveillance program. among the species or species groups with <or=0.14% prevalence (1959 strains tested), doripenem was active against 98.9% of enterobacteriaceae at <or=0.5 microg/ml. similarly, more than 90% of other rarely isolated gram-ne ... | 2009 | 19302927 |
| influence of stress on single-cell lag time and growth probability for listeria monocytogenes in half fraser broth. | the impacts of 12 common food industry stresses on the single-cell growth probability and single-cell lag time distribution of listeria monocytogenes were determined in half fraser broth, the primary enrichment broth of the international organization for standardization detection method. first, it was determined that the ability of a cell to multiply in half fraser broth is conditioned by its history (the probability for a cell to multiply can be decreased to 0.05), meaning that, depending on th ... | 2009 | 19304822 |
| identification of the efflux transporter of the fluoroquinolone antibiotic ciprofloxacin in murine macrophages: studies with ciprofloxacin-resistant cells. | ciprofloxacin, the most widely used totally synthetic antibiotic, is subject to active efflux mediated by a mrp-like transporter in wild-type murine j774 macrophages. to identify the transporter among the seven potential mrps, we used cells made resistant to ciprofloxacin obtained by long-term exposure to increasing drug concentrations (these cells show less ciprofloxacin accumulation and provide a protected niche for ciprofloxacin-sensitive intracellular listeria monocytogenes). in the present ... | 2009 | 19307362 |
| comparison of systemic listeria monocytogenes infection in esophageally inoculated mice anesthetized with isoflurane or pentobarbital. | in previous attempts to produce a murine model of gastrointestinal listeriosis, the authors observed that pentobarbital anesthesia greatly increased disease severity in mice that were esophageally inoculated with the bacterium listeria monocytogenes. in this study, they sought to evaluate the severity of systemic infection in inoculated mice that were anesthetized with isoflurane, an inhalational agent that is safer than pentobarbital and more commonly used for rodent anesthesia. compared with p ... | 2009 | 19308063 |
| necrotic cell death in human amniotic cells infected by listeria monocytogenes. | listeria monocytogenes can cause a placental-foetal infection that results in spontaneous abortion, premature labour, stillbirth, or neonatal sepsis and meningitis. bacteria cross the maternofoetal barrier at the villous syncytiotrophoblast level and subsequently spread from the placenta to the fetus. l. monocytogenes is able to induce different kinds of death in a variety of cells. murine hepatocytes, murine t and human b lymphocytes, and murine dendritic cells die by apoptosis, whereas bacteri ... | 2009 | 19309562 |
| expression of internalin a and biofilm formation among listeria monocytogenes clinical isolates. | internalin a (inla), a cell wall-bound protein of listeria monocytogenes, is among the major components involved in the adhesion to and invasion of host cells expressing specific forms of e-cadherin. some l. monocytogenes strains secrete truncated non-functional forms of inla. the purpose of this study is to compare the biofilm-forming abilities of l. monocytogenes strains from clinical sources expressing inla proteins in the different forms. a total of 70 l. monocytogenes strains were examined ... | 2009 | 19309566 |
| a stably engineered, suicidal strain of listeria monocytogenes delivers protein and/or dna to fully differentiated intestinal epithelial monolayers. | we have generated a recombinant stable, suicidal listeria monocytogenes strain (rsdelta2) capable of delivering antigens as protein or dna into nondividing intestinal epithelial cells. the rsdelta2 strain was generated by inserting a cell wall hydrolysin gene, "ply118" together with its associated holin gene from a listeria-specific phage, into the attenuated l. monocytonegenes genome of strain delta2. the hol118/ply118 gene was placed under the control of the listeria promoter pacta, inducing b ... | 2009 | 19317435 |
| targeted capture of pathogenic bacteria using a mammalian cell receptor coupled with dielectrophoresis on a biochip. | efficient capture of target analyte on biosensor platforms is a prerequisite for reliable and specific detection of pathogenic microorganisms in a microfluidic chip. antibodies have been widely used as ligands; however, because of their occasional unsatisfactory performance, a search for alternative receptors is underway. heat shock protein 60 (hsp60), a eukaryotic mitochondrial chaperon protein is a receptor for listeria adhesion protein (lap) during listeria monocytogenes infection. this paper ... | 2009 | 19317455 |
| in vitro antilisterial effects of citrus oil fractions in combination with organic acids. | the objectives of this study were to screen activity of citrus essential oil fractions (eos) alone and in combination with organic acids against 2 species of listeria. five citrus eos were initially screened by disc diffusion assay for antibacterial activity. cold pressed terpeneless valencia orange oil (cp terpeneless oil) had the strongest bacteriostatic (mic) and bactericidal (mbc) properties at 0.55% and 1.67%, respectively. four organic acids were tested for effectiveness against listeria. ... | 2009 | 19323760 |
| development of a risk-ranking framework to evaluate potential high-threat microorganisms, toxins, and chemicals in food. | through a cooperative agreement with the u.s. food and drug administration, the institute of food technologists developed a risk-ranking framework prototype to enable comparison of microbiological and chemical hazards in foods and to assist policy makers, risk managers, risk analysts, and others in determining the relative public health impact of specific hazard-food combinations. the prototype is a bottom-up system based on assumptions that incorporate expert opinion/insight with a number of ex ... | 2009 | 19323766 |
| cell biology. the art of making an exit. | | 2009 | 19325101 |
| characterization of the interferon-producing cell in mice infected with listeria monocytogenes. | production of type i interferons (ifn-i, mainly ifnalpha and ifnbeta) is a hallmark of innate immune responses to all classes of pathogens. when viral infection spreads to lymphoid organs, the majority of systemic ifn-i is produced by a specialized "interferon-producing cell" (ipc) that has been shown to belong to the lineage of plasmacytoid dendritic cells (pdc). it is unclear whether production of systemic ifn-i is generally attributable to pdc irrespective of the nature of the infecting patho ... | 2009 | 19325882 |
| quantitative risk assessment of listeria monocytogenes in ready-to-eat meats in australia. | listeria monocytogenes is a food-borne pathogen that can contaminate processed meats and has caused outbreaks in several nations in which processed meats were the vehicle. due to its ecology, the control of this organism in ready-to-eat meats is difficult. as a first step in improving risk management for this product:pathogen pair in australia, a stochastic simulation model to predict the numbers of l. monocytogenes likely to be consumed in those products under a wide range of scenarios was deve ... | 2009 | 19327859 |
| subtyping of listeria monocytogenes isolates by acta gene sequencing, pcr-fingerprinting, and cell-invasion assay. | analysis of acta gene sequence polymorphism has been shown to be an effective and relatively inexpensive method for subtyping listeria monocytogenes isolates, allowing the division of the population of this species into two deeply separate lineages. this sequence-based method as well as pcr-mediated fingerprinting were applied here for the differentiation of 49 isolates of food and clinical origin. correlation between these two typing approaches was high. both methods divided the isolates into t ... | 2009 | 19330540 |
| prevention of foodborne listeriosis. | listeria monocytogenes is a foodborne pathogen that can cause serious invasive illness, mainly in certain well-defined high-risk groups, including elderly and immunocompromised patients, pregnant women, newborns and infants. l. monocytogenes primarily causes abortion, septicemia or meningitis. contaminated meats (such as hot dogs, delicatessen meats and patiota), dairy products and seafood have all been implicated in outbreaks of listeriosis. the public health importance of listeriosis is not al ... | 2009 | 19332898 |
| molecular cloning and characterization of taiwan macaque lactoferrin. | lactoferrin (lf) is an iron-binding glycoprotein that plays an important role in combating a wide range of pathogens and contributes innate protective defenses of mammals. | 2009 | 19335476 |
| seasonal relationships among indicator bacteria, pathogenic bacteria, cryptosporidium oocysts, giardia cysts, and hydrological indices for surface waters within an agricultural landscape. | the south nation river basin in eastern ontario, canada is characterized by mixed agriculture. over 1600 water samples were collected on a bi-weekly basis from up to 24 discrete sampling sites on river tributaries of varying stream order within the river basin between 2004 and 2006. water samples were analyzed for: densities of indicator bacteria (escherichia coli, clostridium perfringens, enterococci, total and fecal coliforms), the presence of pathogenic bacteria (listeria monocytogenes, e. co ... | 2009 | 19339033 |
| pd-1 on dendritic cells impedes innate immunity against bacterial infection. | programmed death one (pd-1) is an inducible molecule belonging to the immunoglobulin superfamily. it is expressed on activated t and b lymphocytes and plays pivotal roles in the negative regulation of adaptive immune responses. we report here an unexpected finding: that pd-1 could also be induced on splenic dendritic cells (dcs) by various inflammatory stimuli. adoptive transfer of pd-1-deficient dcs demonstrates their superior capacity to wild-type dcs in innate protection of mice against letha ... | 2009 | 19339692 |
| identifying activated t cells in reconstituted rag deficient mice using retrovirally transduced pax5 deficient pro-b cells. | various methods have been used to identify activated t cells such as binding of mhc tetramers and expression of cell surface markers in addition to cytokine-based assays. in contrast to these published methods, we here describe a strategy to identify t cells that respond to any antigen and track the fate of these activated t cells. we constructed a retroviral double-reporter construct with enhanced green fluorescence protein (egfp) and a far-red fluorescent protein from heteractis crispa (hcred) ... | 2009 | 19340306 |
| suppressor of cytokine signaling 2 (socs-2) homologue in disk abalone: cloning, sequence characterization and expression analysis. | suppressor of cytokine signaling (socs) proteins are inhibitors of cytokine signaling pathways and key physiological regulators of both innate and adaptive immune systems in vertebrates. in present study, we provided the initial characterization of socs-2 homologue from the mollusk abalone haliotis discus discus as a member of invertebrate socs-2. the disk abalone socs-2 cdna (absocs-2) contains 1700-bp full length with 771-bp coding sequence, which codes 257 amino acids protein. based on the se ... | 2009 | 19340953 |
| early infection termination affects number of cd8+ memory t cells and protective capacities in listeria monocytogenes-infected mice upon rechallenge. | here, we reevaluate the effects of early termination of infection on primary t cell expansion, subsequent memory cell development, and protective immunity. using a murine listeria monocytogenes (lm) infection model, we found the primary expansions of both cd4(+) and cd8(+) t cells were affected even when ampicillin was given as late as 60 h postinfection (p.i.). subsequent development of cd8(+) memory t cells was also impaired, although to a lesser extent, and only mice that received ampicillin ... | 2009 | 19342633 |
| characterization of a bacteriocin produced by enterococcus faecalis n1-33 and its application as a food preservative. | a bacteriocin-producing strain, n1-33, isolated from fermented bamboo shoot was identified as enterococcus faecalis. the ph-adjusted culture supernatant of this strain consisted of several peptides with bacteriocin activity, and the supernatant inhibited the growth of pathogenic bacteria such as listeria monocytogenes. the major peptide with bacteriocin activity was purified, and the first 39 amino acid residues of the bacteriocin were found to be identical to enterocin mr10a produced by e. faec ... | 2009 | 19343940 |
| fate of listeria monocytogenes on fully ripened greek graviera cheese stored at 4, 12, or 25 degrees c in air or vacuum packages: in situ pcr detection of a cocktail of bacteriocins potentially contributing to pathogen inhibition. | the behavior of listeria monocytogenes on fully ripened greek graviera cheese was evaluated. three batches (a, b, and c) were tested. batches a and c were prepared with a commercial starter culture, while in batch b the starter culture was combined with an enterocin-producing enterococcus faecium graviera isolate. cheese pieces were surface inoculated with a five-strain cocktail of l. monocytogenes at ca. 3 log cfu/cm2, packed under air or vacuum conditions, stored at 4, 12, or 25 degrees c, and ... | 2009 | 19343941 |
| fate of food-associated bacteria in pork as affected by marinade, temperature, and ultrasound. | the aim of this study was to test the effect of ultrasound, red wine, and yogurt marination on brochotrix thermosphacta, carnobacterium maltaromaticum, listeria monocytogenes, and campylobacter jejuni on pork meat. two different marination procedures of the pork medallions were tested: (i) submersion of meat medallions in red wine during the entire experiment and (ii) vacuum packaging of meat medallions after different forms of marination. in the submersion procedure, the meat was either submerg ... | 2009 | 19343943 |
| impact of storage temperature and product ph on the survival of listeria monocytogenes in vacuum-packaged souse. | souse is a fully cooked, ready-to-eat gelled pork product. there is a zero-tolerance policy for listeria monocytogenes in ready-to-eat meat products. the survival and/or growth of l. monocytogenes in souse is unknown. the effectiveness of three different souse formulations (ph 4.3, 4.7, and 5.1) for controlling the growth of l. monocytogenes at two refrigerated storage temperatures (5 and 10 degrees c) was evaluated. all products were vacuum packaged. uninoculated product was prepared as the con ... | 2009 | 19343956 |
| radiation sensitization and postirradiation proliferation of listeria monocytogenes on ready-to-eat deli meat in the presence of pectin-nisin films. | in this study, the ability of pectin-nisin films in combination with ionizing radiation to eliminate listeria monocytogenes and inhibit its postirradiation proliferation was evaluated. pectin films containing 0.025% nisin were made by extrusion. the surface of a ready-to-eat turkey meat sample was inoculated with l. monocytogenes at 10(6) cfu/cm2 and covered with a piece of pectin-nisin film. the samples were vacuum packaged and irradiated at 0, 1, and 2 kgy. the treated samples were stored at 1 ... | 2009 | 19343957 |
| increased detection of listeria species and listeria monocytogenes in raw beef, using the assurance gds molecular detection system with culture isolation. | testing for listeria is challenging because of its slow growth rate. recently, we described a rapid listeria culture isolation method. this method can be improved by utilizing a rapid molecular detection test such as the assurance gds tests for listeria and listeria monocytogenes. these two methods (culture isolation and assurance gds) use different enrichment strategies that may affect the number of listeria and l. monocytogenes cells detected. therefore, after first determining that the assura ... | 2009 | 19343963 |
| seropositivity for listeria monocytogenes in women with spontaneous abortion: a case-control study in iran. | there are many studies supporting the role of certain asymptomatic infections such as listeria monocytogenes (l. monocytogenes) in spontaneous abortion. in some cases, latent listeriosis may complicate the pregnancy, and serologic tests can, therefore, be used to detect the disease. this study was designed to assess the relationship between seropositivity for l. monocytogenes and spontaneous abortion. | 2009 | 19346191 |
| the propeptide of the metalloprotease of listeria monocytogenes controls compartmentalization of the zymogen during intracellular infection. | integral to the virulence of the intracellular bacterial pathogen listeria monocytogenes is its metalloprotease (mpl). mpl regulates the activity and compartmentalization of the bacterial broad-range phospholipase c (pc-plc). mpl is secreted as a proprotein that undergoes intramolecular autocatalysis to release its catalytic domain. in related proteases, the propeptide serves as a folding catalyst and can act either in cis or in trans. propeptides can also influence protein compartmentalization ... | 2009 | 19346305 |
| bacillus anthracis edema toxin impairs neutrophil actin-based motility. | inhalation anthrax results in high-grade bacteremia and is accompanied by a delay in the rise of the peripheral polymorphonuclear neutrophil (pmn) count and a paucity of pmns in the infected pleural fluid and mediastinum. edema toxin (et) is one of the major bacillus anthracis virulence factors and consists of the adenylate cyclase edema factor (ef) and protective antigen (pa). relatively low concentrations of et (100 to 500 ng/ml of pa and ef) significantly impair human pmn chemokinesis, chemot ... | 2009 | 19349425 |
| listeria monocytogenes serovar 4a is a possible evolutionary intermediate between l. monocytogenes serovars 1/2a and 4b and l. innocua. | the genus listeria consists of six closely related species and forms three phylogenetic groups: l. monocytogenes- l. innocua, l. ivanovii-l. seeligeri-l. welshimeri, and l. grayi. in this report, we attempted to examine the evolutionary relationship in the l. monocytogenes-l. innocua group by probing the nucleotide sequences of 23s rrna and 16s rrna, and the gene clusters lmo0029-lmo0042, ascbdape, rpls-infc, and prs-ldh in l. monocytogenes serovars 1/2a, 4a, and 4b, and l. innocua. additionally ... | 2009 | 19349748 |
| evaluation of treatments for elimination of foodborne pathogens on the surface of leaves and roots of lettuce (lactuca sativa l.). | several outbreaks of salmonella and escherichia coli o157:h7 infections have been associated with consumption of leafy greens. questions remain concerning the ability of these pathogens to become internalized within lettuce and spinach tissues. an effective validated surface disinfection method for lettuce is needed before factors affecting internalization of pathogens can be studied. the objective of this study was to develop a surface disinfection method for lettuce leaves and roots. iceberg l ... | 2009 | 19350966 |
| reduction of listeria on ready-to-eat sausages after exposure to a combination of pulsed light and nisin. | the risk of listeriosis associated with ready-to-eat foods is a major concern in the united states. pulsed light (pl) treatment has been effective for killing listeria. the possibility of enhancing the antilisterial capability of pl treatment by combining pl with an additional hurdle, the natural antimicrobial nisin, was explored in this study. first, the ability of listeria innocua to mimic the response of listeria monocytogenes to pl treatment was demonstrated. subsequently, a series of inocul ... | 2009 | 19350979 |
| psychrotrophic lactic acid bacteria used to improve the safety and quality of vacuum-packaged cooked and peeled tropical shrimp and cold-smoked salmon. | previously isolated lactic acid bacteria (lab) from seafood products have been investigated for their capacity to increase the sensory shelf life of vacuum-packaged shrimp and cold-smoked salmon and to inhibit the growth of three pathogenic bacteria. two different manufactured batches of cooked, peeled, and vacuum-packaged shrimp were inoculated with seven lab strains separately at an initial level of 5 log cfu g-t, and the spoilage was estimated by sensory analysis after 7 and 28 days of storag ... | 2009 | 19350982 |
| fate of surface-inoculated escherichia coli o157:h7, listeria monocytogenes, and salmonella typhimurium on kippered beef during extended storage at refrigeration and abusive temperatures. | the behavior of escherichia coli o157:h7, listeria monocytogenes, and salmonella typhimurium on kippered beef was evaluated. individual pieces of the product were separately inoculated on the top and bottom surfaces with each three- to six-strain pathogen cocktail at ca. 6.0 log cfu per piece and stored at 4, 10, 21, or 30 degrees c for up to 28 days in each of two trials. when kippered beef was inoculated with e. coli o157:h7, salmonella typhimurium, or l. monocytogenes and stored at 4, 10, 21, ... | 2009 | 19350987 |
| pilot-scale continuous ultrasonic cleaning equipment reduces listeria monocytogenes levels on conveyor belts. | ultrasonic cleaning of a conveyor belt was studied by building a pilot-scale conveyor with an ultrasonic cleaning bath. a piece of the stainless steel conveyor belt was contaminated with meat-based soil and listeria monocytogenes strains (v1, v3, and b9) and incubated for 72 h to allow bacteria to attach to the conveyor belt surfaces. the effect of ultrasound with a potassium hydroxide-based cleaning detergent was determined by using the cleaning bath at 45 and 50 degrees c for 30 s with and wit ... | 2009 | 19350988 |
| innate immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections. | neutrophils are the most abundant leukocytes in circulation and provide a primary innate immune defense function against bacterial pathogens before development of a specific immune response. these specialized phagocytes are short lived (12-24 hours) and continuously replenished from bone marrow. we found that if the host is overwhelmed by a high inoculum of listeria monocytogenes, neutrophils are depleted despite high granulocyte-colony stimulating factor induction. in contrast to a low-dose inn ... | 2009 | 19351895 |
| study of the effect of lethal and sublethal ph and a(w) stresses on the inactivation or growth of listeria monocytogenes and salmonella typhimurium. | during food processing, microorganisms are commonly exposed to multiple sublethal or lethal stresses (commonly a(w), ph) sequentially or simultaneously. the objectives of the present study were: (i) to comparatively evaluate the survival of listeria monocytogenes and salmonella typhimurium in lethal acid (ph 4.0 and 4.5 with lactic acid) or osmotic conditions (15 and 20% nacl), applied singly, sequentially (ph then nacl or nacl then ph), or simultaneously at 5 and 10 degrees c; and, (ii) to quan ... | 2009 | 19356819 |
| the use of flagella and motility for plant colonization and fitness by different strains of the foodborne pathogen listeria monocytogenes. | the role of flagella and motility in the attachment of the foodborne pathogen listeria monocytogenes to various surfaces is mixed with some systems requiring flagella for an interaction and others needing only motility for cells to get to the surface. in nature this bacterium is a saprophyte and contaminated produce is an avenue for infection. previous studies have documented the ability of this organism to attach to and colonize plant tissue. motility mutants were generated in three wild type s ... | 2009 | 19357783 |
| antilisterial activities of salad dressings, without or with prior microwave oven heating, on frankfurters during simulated home storage. | | 2009 | 19359060 |
| tina wooden vat biofilm: a safe and highly efficient lactic acid bacteria delivering system in pdo ragusano cheese making. | in the sicilian pdo ragusano cheese making, raw milk is placed in a wooden vat called a tina. as no starter is added, lactic acid is produced by milk flora and flora released from the tina biofilm. the aim of this work was to assess the safety and efficiency of this natural inoculation system. from 15 tinas' biofilms, bacteria total counts varied from 10(3) to 10(6) cfu/cm(2), with the predominance of thermophilic lactic acid bacteria. low counts of yeasts and moulds were found in a few tinas. s ... | 2009 | 19361876 |
| combination of daptomycin plus ceftriaxone is more active than vancomycin plus ceftriaxone in experimental meningitis after addition of dexamethasone. | we examined the cerebrospinal fluid penetration of daptomycin after the addition of dexamethasone and its bactericidal efficacy with and without ceftriaxone in an experimental rabbit model of pneumococcal meningitis. the combination of daptomycin with ceftriaxone was the most efficacious regimen for pneumococcal meningitis. the previous addition of dexamethasone affected the antibacterial activity of daptomycin only marginally, either as monotherapy or combined with ceftriaxone, although the pen ... | 2009 | 19364870 |
| construction of a recombinant intergenus multidomain chimeric protein for simultaneous expression of haemolysin bl of bacillus cereus, listeriolysin o of listeria monocytogenes and enterotoxin b of staphylococcus aureus. | haemolysin bl (hbl) of bacillus cereus, listeriolysin o (llo) of listeria monocytogenes and enterotoxin b (seb) of staphylococcus aureus are among the major toxin components contributing to the pathogenicity of these organisms in foodborne illnesses. in this study, an intergenus non-toxic multidomain fusion protein (r-hle) was generated with specificity for hbl, llo and seb. the fusion gene (r-hle) comprising the conserved regions of hbld and the hly and entb genes was codon-optimized for expres ... | 2009 | 19369518 |
| life on the inside: the intracellular lifestyle of cytosolic bacteria. | bacterial pathogens exploit a huge range of niches within their hosts. many pathogens can invade non-phagocytic cells and survive within a membrane-bound compartment. however, only a small number of bacteria, including listeria monocytogenes, shigella flexneri, burkholderia pseudomallei, francisella tularensis and rickettsia spp., can gain access to and proliferate within the host cell cytosol. here, we discuss the mechanisms by which these cytosolic pathogens escape into the cytosol, obtain nut ... | 2009 | 19369949 |
| antimicrobial mechanisms of phagocytes and bacterial evasion strategies. | professional phagocytes have a vast and sophisticated arsenal of microbicidal features. they are capable of ingesting and destroying invading organisms, and can present microbial antigens on their surface, eliciting acquired immune responses. to survive this hostile response, certain bacterial species have developed evasive strategies that often involve the secretion of effectors to co-opt the cellular machinery of the host. in this review, we present an overview of the antimicrobial defences of ... | 2009 | 19369951 |
| listeriolysin o affects barrier function and induces chloride secretion in ht-29/b6 colon epithelial cells. | listeria monocytogenes is a food-borne pathogen, which is able to induce diarrhea when residing in the intestine. we studied the effect of listeriolysin o (llo), an extracellular virulence factor of l. monocytogenes, on intestinal transport and barrier function in monolayers of ht-29/b6 human colon cells using the ussing technique to understand the pathomechanisms involved. mucosal addition of llo, but not a llo mutant, induced a dose- and ph-dependent increase in short-circuit current (i(sc)). ... | 2009 | 19372105 |
| prevalence and concentration of verocytotoxigenic escherichia coli, salmonella enterica and listeria monocytogenes in the beef production chain: a review. | this review examines the prevalence of three important pathogens, verocytotoxigenic escherichia coli (vtec), salmonella enterica and listeria monocytogenes, in cattle and beef from the farm to the final, ready-to-eat product. factors affecting prevalence of pathogens in the beef chain, such as the season and cattle rearing method, are examined. data from many key surveys are summarized in table form. the observed prevalence of pathogens in cattle and beef varies considerably from survey to surve ... | 2009 | 19376457 |
| the decontaminative effects of acidic electrolyzed water for escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes on green onions and tomatoes with differing organic demands. | acidic electrolyzed water (ac-ew) has strong bactericidal activity against foodborne pathogens on fresh vegetables. however, the efficacy of ac-ew is influenced by soil or other organic materials present. this study examined the bactericidal activity of ac-ew in the presence of organic matter, in the form of bovine serum against foodborne pathogens on the surfaces of green onions and tomatoes. green onions and tomatoes were inoculated with a culture cocktail of escherichia coli o157:h7, salmonel ... | 2009 | 19376459 |
| differences in pressure tolerance of listeria monocytogenes strains are not correlated with other stress tolerances and are not based on differences in ctsr. | thirty strains of listeria monocytogenes were screened for their pressure tolerance phenotype at 400 mpa for 2 min at 21 degrees c. the strains exhibited reductions ranging from 1.9 to 7.1 log(10)cfu/ml in tryptic soy broth with 6% yeast extract (tsbye). the 3 most and the 3 least pressure-tolerant strains were further tested for their thermal resistance (based on their ability to survive at 55 degrees c), acid tolerance (based on their ability to survive in acidified tsbye; ph 2.0) and for thei ... | 2009 | 19376462 |
| evaluation of atmospheric pressure plasma to improve the safety of sliced cheese and ham inoculated by 3-strain cocktail listeria monocytogenes. | the objective of this study was to evaluate the efficacy of atmospheric pressure plasma (app), which is capable of operating at atmospheric pressure in air, in sliced cheese and ham inoculated by 3-strain cocktail of listeria monocytogenes (atcc 19114, 19115, and 19111, lmc). the process parameters considered were input power (75, 100, 125, and 150 w) and plasma exposure time (60, 90, and 120 s). microbial log reduction increased with increases of input power and plasma exposure time. after 120 ... | 2009 | 19376467 |
| development of a mariner-based transposon and identification of listeria monocytogenes determinants, including the peptidyl-prolyl isomerase prsa2, that contribute to its hemolytic phenotype. | listeriolysin o (llo) is a pore-forming toxin that mediates phagosomal escape and cell-to-cell spread of the intracellular pathogen listeria monocytogenes. in order to identify factors that control the production, activity, or secretion of this essential virulence factor, we constructed a himar1 mariner transposon delivery system and screened 50,000 mutants for a hypohemolytic phenotype on blood agar plates. approximately 200 hypohemolytic mutants were identified, and the 51 most prominent mutan ... | 2009 | 19376879 |
| effect of octenidine hydrochloride on planktonic cells and biofilms of listeria monocytogenes. | listeria monocytogenes is a food-borne pathogen capable of forming biofilms and persisting in food processing environments for extended periods of time, thereby potentially contaminating foods. the efficacy of octenidine hydrochloride (oh) for inactivating planktonic cells and preformed biofilms of l. monocytogenes was investigated at 37, 21, 8, and 4 degrees c in the presence and absence of organic matter (rehydrated nonfat dry milk). oh rapidly killed planktonic cells and biofilms of l. monocy ... | 2009 | 19376913 |
| differential listeria monocytogenes strain survival and growth in katiki, a traditional greek soft cheese, at different storage temperatures. | katiki domokou is a traditional greek cheese, which has received the protected designation of origin recognition since 1994. its microfloras have not been studied although its structure and composition may enable (or even favor) the survival and growth of several pathogens, including listeria monocytogenes. the persistence of l. monocytogenes during storage at different temperatures has been the subject of many studies since temperature abuse of food products is often encountered. in the present ... | 2009 | 19376914 |
| an anti-vascular endothelial growth factor receptor 2/fetal liver kinase-1 listeria monocytogenes anti-angiogenesis cancer vaccine for the treatment of primary and metastatic her-2/neu+ breast tumors in a mouse model. | thirty years after angiogenesis was shown to play an enabling role in cancer, modern medicine is still trying to develop novel compounds and therapeutics to target the tumor vasculature. however, most therapeutics require multiple rounds of administration and can have toxic side effects. in this study, we use anti-angiogenesis immunotherapy to target cells actively involved in forming new blood vessels that support the growth and spread of breast cancer. targeting a central cell type involved in ... | 2009 | 19380802 |
| association between a case study of asymptomatic ovine listerial mastitis and the contamination of soft cheese and cheese processing environment with listeria monocytogenes in portugal. | for 5 months, the udders of milking ewes, raw ewe's milk, cheese, and the plant and environment of a cheese manufacturer in portugal were investigated using standard methods for the presence of listeria spp. an association between subclinical mastitis and listeria monocytogenes in a single lactating sheep was investigated by visual inspection of udders for signs of inflammation, application of somatic cell counts, the california mastitis test, ph measurement to milk, and culture of l. monocytoge ... | 2009 | 19388828 |
| the innate immunity role of cathepsin-d is linked to trp-491 and trp-492 residues of listeriolysin o. | listeriolysin o (llo) is a thiol-activated cytolysin secreted by listeria monocytogenes. llo and phosphatidylinositol phospholipase c are two essential virulence factors, which this bacterium needs to escape from the phagosomal compartment to the cytoplasm. cathepsin-d specifically cleaves llo, between the trp-491 (tryptophan amino acid in three letter nomenclature) and trp-492 residues of the conserved undecapeptide sequence, ectglawewwr, in the domain 4 of llo (d4). moreover, these residues al ... | 2009 | 19389128 |
| the first clinical use of a live-attenuated listeria monocytogenes vaccine: a phase i safety study of lm-llo-e7 in patients with advanced carcinoma of the cervix. | invasive carcinoma of the cervix (icc) is the second most common cancer in women worldwide. lm-llo-e7 vaccine is a live-attenuated listeria monocytogenes (lm) that secretes the hpv-16 e7 antigen fused to a non-hemolytic fragment of the lm protein listeriolysin o (llo). in this phase i trial, the safety of lm-llo-e7 was assessed in 15 patients with previously treated metastatic, refractory or recurrent icc. patients received 1 of 3 dose levels of lm-llo-e7 (1 x 10(9)cfu, 3.3 x 10(9)cfu or 1 x 10( ... | 2009 | 19389451 |
| il-15 protects antigen-specific cd8+ t cell contraction after mycobacterium bovis bacillus calmette-guérin infection. | we reported previously that il-15 plays a critical role in protecting effector cd8(+) t cells from apoptosis during the contraction phase following acute infection with listeria monocytogenes by inducing antiapoptotic molecules. in the present study, we examined the effects of in vivo administration of ril-15 on contraction of cd8(+) t cells after chronic infection with mycobacterium bovis bcg and on the efficacy of bcg vaccination against mycobacterium tuberculosis infection. antigen-specific c ... | 2009 | 19389797 |
| mechanics model for actin-based motility. | we present here a mechanics model for the force generation by actin polymerization. the possible adhesions between the actin filaments and the load surface, as well as the nucleation and capping of filament tips, are included in this model on top of the well-known elastic brownian ratchet formulation. a closed form solution is provided from which the force-velocity relationship, summarizing the mechanics of polymerization, can be drawn. model predictions on the velocity of moving beads driven by ... | 2009 | 19391787 |
| [contribution of microbiological methods to the diagnosis of acute bacterial meningitis]. | the most frequent bacteria responsible for acute bacterial meningitis, after the neonatal period, are meningoccoci and pneumococci, very rarely haemophilus influenzae and listeria monocytogenes. the microbiological diagnosis is based on cell count, gram stain, and culture of cerebrospinal fluid. antigen detection and dna detection are useful to identify the bacteria in cases of negative cultures, because of the fragility of some bacterial species (meningococci), or a prior antibiotic administrat ... | 2009 | 19394176 |
| [epidemiology of acute bacterial meningitis in adult patients in france]. | in 2006, the number of bacterial meningitis cases was estimated at 1375 (2.23/100,000). the leading pathogens involved in adult meningitis were, according to frequency, streptococcus pneumoniae, neisseria meningitidis, listeria monocytogenes, streptococcus agalactiae, and haemophilus influenzae. the overall mortality rate averaged 20%, higher among patients with pneumococcal meningitis or in individuals over 65 years of age. sequels were observed in 30% of cases and more frequent after pneumococ ... | 2009 | 19395209 |
| inhibition of listeria monocytogenes by a combination of chitosan and divergicin m35. | the antimicrobial activities of the class iia bacteriocin divergicin m35 and several types of chitosan against listeria monocytogenes were quantified by agar diffusion, critical micro-dilution, and viable count and observed by electron microscopy. antimicrobial activity of chitosan depended on its molecular mass (mm) and the ph. three chitosans with mm values of 2, 20, and 100 kda and 87.4% degree of deacetylation (dda) were chosen for further study, based on high anti-listerial activity at ph 4 ... | 2009 | 19396234 |
| rapid detection of eight causative pathogens for the diagnosis of bacterial meningitis by real-time pcr. | we aimed to detect causative pathogens in cerebrospinal fluid (csf) collected from patients diagnosed with bacterial meningitis by real-time polymerase chain reaction (pcr). in addition to streptococcus pneumoniae, haemophilus influenzae, and mycoplasma pneumoniae described previously, five other pathogens, neisseria meningitidis, escherichia coli, streptococcus agalactiae, staphylococcus aureus, and listeria monocytogenes, were targeted, based on a large-scale surveillance in japan. results in ... | 2009 | 19396518 |
| ultraviolet light (254 nm) inactivation of listeria monocytogenes on frankfurters that contain potassium lactate and sodium diacetate. | listeria monocytogenes, a psychrotrophic foodborne pathogen, is an occasional postprocess contaminant on ready-to-eat meat (rte) products including frankfurters. ultraviolet c light (uvc) is an fda-approved technology for the decontamination of food surfaces. in this study, the ability of uvc to inactivate l. monocytogenes on frankfurters that contained potassium lactate (pl) and sodium diacetate (sda), either before or after packaging, was investigated. uvc irradiation of frankfurters that were ... | 2009 | 19397726 |
| modeling the growth characteristics of listeria monocytogenes and native microflora in smoked salmon. | smoked salmon contaminated with listeria monocytogenes has been implicated in foodborne listeriosis. the objectives of this study were to model the growth characteristics and examine the growth relationship of l. monocytogenes and native microflora in smoked salmon. smoked salmon samples with a native microflora count of 2.9 log(10) cfu/g were inoculated with a 6-strain mixture of l. monocytogenes to levels of log(10) 1.6 and log(10) 2.8 cfu/g, and stored at 4, 8, 12, and 16 degrees c. growth ch ... | 2009 | 19397728 |
| inactivation of listeria innocua on frankfurters by ultraviolet light and flash pasteurization. | listeria monocytogenes, a psychrotrophic foodborne pathogen, is a recurring postprocess contaminant on ready-to-eat meat (rte) products including frankfurters. flash (steam) pasteurization (fp) and ultraviolet light (254 nm-uvc) has been shown to reduce levels of l. monocytogenes and l. innocua on frankfurters. in this study, the use of uvc light followed by fp to inactivate l. innocua, a nonpathogenic surrogate for l.monocytogenes, on frankfurters that contained sodium diacetate and potassium l ... | 2009 | 19397730 |
| [antibiotic management of presumptive bacterial meningitis in adults (rational, methods, course, and follow-up)]. | the annual incidence of community acquired meningitis ranges between 0.6 and four per 100,000 adults in industrialized countries. the most common causative bacteria are streptococcus pneumoniae, neisseria meningitidis, listeria monocytogenes. the emergence of resistance to antibiotics, especially for s. pneumoniae, could explain the clinical failure of third generation cephalosporins used to treat adults with s. pneumoniae meningitis. the present therapeutic suggestions are more based on the ext ... | 2009 | 19398287 |
| nonhematopoietic cells control the outcome of infection with listeria monocytogenes in a nucleotide oligomerization domain 1-dependent manner. | we analyzed the defensive role of the cytosolic innate recognition receptor nucleotide oligomerization domain 1 (nod1) during infection with listeria monocytogenes. mice lacking nod1 showed increased susceptibility to systemic intraperitoneal and intravenous infection with high or low doses of l. monocytogenes, as measured by the bacterial load and survival. nod1 also controlled dissemination of l. monocytogenes into the brain. the increased susceptibility to reinfection of nod1(-/-) mice was no ... | 2009 | 19398545 |
| listeria monocytogenes 6-phosphogluconolactonase mutants induce increased activation of a host cytosolic surveillance pathway. | infection with wild-type listeria monocytogenes activates a host cytosolic surveillance response characterized by the expression of beta interferon (ifn-beta). we performed a genetic screen to identify l. monocytogenes transposon insertion mutants that induced altered levels of host ifn-beta expression. one mutant from this screen induced elevated levels of ifn-beta and harbored a tn917 insertion upstream of lmo0558. this study identified lmo0558 as the 6-phosphogluconolactonase gene (pgl), whic ... | 2009 | 19398547 |
| myeloid heme oxygenase-1 regulates innate immunity and autoimmunity by modulating ifn-beta production. | heme oxygenase-1 (ho-1) is a key cytoprotective, antioxidant, and antiinflammatory molecule. the pathophysiological functions of ho-1 have been associated with its enzymatic activities in heme catabolism. we have examined the immune functions of ho-1 by its conditional ablation in myeloid cells (ho-1(m-ko) mice). we demonstrate that myeloid ho-1 is required for the activation of interferon (ifn) regulatory factor (irf) 3 after toll-like receptor 3 or 4 stimulation, or viral infection. ho-1-defic ... | 2009 | 19398754 |
| the anti-inflammatory non-antibiotic helper compound diclofenac: an antibacterial drug target. | diclofenac sodium (dc) was found to possess antibacterial activity against both drug-sensitive and drug-resistant clinical isolates of staphylococcus aureus, listeria monocytogenes, escherichia coli, and mycobacterium spp., in addition to its potent anti-inflammatory activity. the time-kill curve study indicates that this non-steroidal drug exhibits bactericidal activity against listeria, e. coli, and m. tuberculosis. the antibacterial activity of dc comes, in part, from its ability to inhibit t ... | 2009 | 19399540 |
| characterization of a small erythromycin resistance plasmid plfe1 from the food-isolate lactobacillus plantarum m345. | this paper reports the complete 4031 bp nucleotide sequence of the small erythromycin resistance plasmid plfe1 isolated from the raw-milk cheese isolate lactobacillus plantarum m345. analysis of the sequence revealed the coding regions for the erythromycin resistance determinant erm(b). a replication initiation protein repb was identified belonging to the repb proteins of the pmv158 family of rolling-circle replicating plasmids. the transcriptional repressor protein copg and a small counter tran ... | 2009 | 19399991 |
| risk factors for mortality among patients with nonperinatal listeriosis in los angeles county, 1992-2004. | listeriosis is a relatively rare foodborne disease with significant public health implications. the causative pathogen, listeria monocytogenes, grows well in refrigeration, is associated with a case-fatality rate of 20%, and causes an estimated 28% of all foodborne disease-related deaths. nevertheless, data on the risk factors for listeriosis mortality are limited. | 2009 | 19400687 |