| endophytic bacteria from piper tuberculatum jacq.: isolation, molecular characterization, and in vitro screening for the control of fusarium solani f. sp piperis, the causal agent of root rot disease in black pepper (piper nigrum l.). | endophytic bacteria have been found to colonize internal tissues in many different plants, where they can have several beneficial effects, including defense against pathogens. in this study, we aimed to identify endophytic bacteria associated with roots of the tropical piperaceae piper tuberculatum, which is known for its resistance to infection by fusarium solani f. sp piperis, the causal agent of black pepper (piper nigrum) root rot disease in the amazon region. based on 16s rrna gene sequence ... | 2015 | 26214435 |
| whole-cell-based identification of electrochemically active bacteria in microbial fuel cells by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | electrochemically active bacteria (eab) that are capable of producing electricity from renewable biomass and organic wastes have been of particular interest in recent years. methods for selective enrichment, accurate identification and easy acquisition of eab fingerprints for phylogenetic characterization would facilitate utilization of these bioenergy-producing species in practical environmental engineering applications. | 2015 | 26522312 |
| a novel d-mandelate dehydrogenase used in three-enzyme cascade reaction for highly efficient synthesis of non-natural chiral amino acids. | a novel nad(+)-dependent d-mandelate dehydrogenase was identified from lactobacillus brevis (lbdmdh). after purified to homogeneity, the optimum ph and temperature for oxidation of d-mandelate were ph 10.0 and 40 °c, and the km and kcat were 1.1 mm and 355 s(-1) respectively. employing the lbdmdh together with a mandelate racemase from pseudomonas putida and a leucine dehydrogenase (esleudh) from exiguobacterium sibiricum, we established a three-step one-pot domino reaction system for preparing ... | 2015 | 25449542 |
| microbial background flora in small-scale cheese production facilities does not inhibit growth and surface attachment of listeria monocytogenes. | the background microbiota of 5 norwegian small-scale cheese production sites was examined and the effect of the isolated strains on the growth and survival of listeria monocytogenes was investigated. samples were taken from the air, food contact surfaces (storage surfaces, cheese molds, and brine) and noncontact surfaces (floor, drains, and doors) and all isolates were identified by sequencing and morphology (mold). a total of 1,314 isolates were identified and found to belong to 55 bacterial ge ... | 2013 | 23891302 |
| microbial diversity and dynamics of microbial communities during back-slop soaking of soybeans as determined by pcr-dgge and molecular cloning. | tempe is a traditional fermented food in indonesia. the manufacture process is quite complex, which comprises two stages, preparatory soaking of soybeans and fungal solid state fermentation. daily addition of previous soak water (back-slopping) during the soybean soaking step is considered to be crucial in the manufacture of high quality tempe. the microbial diversity and dynamics of the microbial communities evolving during back-slop soaking of soybeans for tempe making was investigated by cult ... | 2013 | 23576016 |
| isolation and characterisation of rhizosphere bacteria active against meloidogyne incognita, phytophthora nicotianae and the root knot-black shank complex in tobacco. | the use of dually antagonistic bacteria (dab) as alternatives to chemicals for biological control of disease complexes has received little attention. in this study targeting the meloidogyne incognita-phytophthora nicotianae complex, dab from the tobacco rhizosphere were identified and screened against the diseases caused by one or both pathogens in tobacco. | 2015 | 24799254 |
| two different primary oxidation mechanisms during biotransformation of thymol by gram-positive bacteria of the genera nocardia and mycobacterium. | thymol has antibacterial, antifungal, insecticidal, and antioxidative properties which are the basis for the wide use of this compound in the cosmetic, food, and pharmaceutical industries. although thymol is a ubiquitously occurring substance in the environment, data about its degradation and detoxification by bacteria are sparse. here, we show the existence of two different pathways for the biotransformation of thymol by nocardia cyriacigeorgica and mycobacterium neoaurum which were described f ... | 2013 | 22828982 |
| the kinetic characterization and x-ray structure of a putative benzoylformate decarboxylase from m. smegmatis highlights the difficulties in the functional annotation of thdp-dependent enzymes. | benzoylformate decarboxylase (bfdc) is a thiamin diphosphate (thdp)-dependent enzyme that catalyzes the nonoxidative decarboxylation of benzoylformate. it is the penultimate enzyme in both the mandelate pathway and the d-phenylglycine degradation pathway. the thdp-dependent enzyme engineering database (teed) now lists more than 800 sequences annotated as bfdcs, including one from mycobacterium smegmatis (msbfdc). however, there is no evidence that either pathway for benzoylformate formation exis ... | 2015 | 25936776 |
| structure, interactions and evolutionary implications of a domain-swapped lectin dimer from mycobacterium smegmatis. | crystal structure determination of the lectin domain of msmeg_3662 from mycobacterium smegmatis and its complexes with mannose and methyl-α-mannose, the first effort of its kind on a mycobacterial lectin, reveals a structure very similar to β-prism ii fold lectins from plant sources, but with extensive unprecedented domain swapping in dimer formation. the two subunits in a dimer often show small differences in structure, but the two domains, not always related by 2-fold symmetry, have the same s ... | 2014 | 24957055 |
| development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals. | rapid, low-cost, and user-friendly strategies are urgently needed for early disease diagnosis and timely treatment, particularly for on-site screening of pathogens in aquaculture. in this study, we successfully developed a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp), which was capable of simultaneously detecting 10 pathogenic bacteria in aquatic animals, i.e., nocardia seriolae, pseudomonas putida, streptococcus iniae, ... | 2014 | 24954661 |
| improving the affinity and activity of cyp101d2 for hydrophobic substrates. | cyp101d2 is a cytochrome p450 monooxygenase from novosphingobium aromaticivorans which is closely related to cyp101a1 (p450cam) from pseudomonas putida. both enzymes selectively hydroxylate camphor to 5-exo-hydroxycamphor, and the residues that line the active sites of both enzymes are similar including the pre-eminent tyr96 residue. however, met98 and leu253 in cyp101d2 replace phe98 and val247 in cyp101a1, and camphor binding only results in a maximal change in the spin state to 40 % high-spin ... | 2013 | 22820521 |
| valvulopathy consistent with endocarditis in an argentine boa (boa constrictor occidentalis). | an argentine boa (boa constrictor occidentalis) of 5 yr 7 mo of age was presented for respiratory problems and regurgitation. radiographs revealed evidence of cardiomegaly and pneumonia. blood smear examination revealed the presence of intracytoplasmic inclusion bodies in peripheral lymphocytes, consistent with inclusion body disease. cultures of a tracheal wash sample resulted in growth of ochrobactrum intermedium and pseudomonas putida. echocardiographic examination revealed a large vegetative ... | 2015 | 25831585 |
| denitrification of industrial wastewater: influence of glycerol addition on metabolic activity and community shifts in a microbial consortium. | the wastewater originating from explosives manufacturing plants are characterized by a high concentration of nitrates (3200mgnl(-1)), sulfates (1470mgl(-1)) and low ph (1.5) as well as the presence of organic compounds, such as nitroglycerin (1.9mgl(-1)) and nitroglycol (4.8mgl(-1)). the application of glycerol (c/n=3) at such a high concentration enabled complete removal of nitrates and did not cause the anaerobic glycerol metabolic pathway of the dnc4 consortium to activate, as confirmed by th ... | 2013 | 24161581 |
| heterologous expression of a photorhabdus luminescens syrbactin-like gene cluster results in production of the potent proteasome inhibitor glidobactin a. | syrbactins are cyclic peptide derivatives which are known to inhibit the eukaryotic proteasome by irreversible covalent binding to its catalytic sites. the only two members of this family characterized to date, syringolin a and glidobactin a, are secreted by certain strains of pseudomonas syringae pv. syringae and strain k481-b101 from the order burkholderiales, respectively. syrbactins are the products of mixed non-ribosomal peptide/polyketide synthases encoded by gene clusters with a character ... | 2013 | 23079192 |
| bacteria can proliferate in thawed cryoprecipitate stored at room temperature for longer than 4 h. | although key coagulation factor activities are maintained in thawed cryoprecipitate stored for up to 24 h at ambient temperature, several jurisdictions limit such storage to 4-6 h. here, we separately spiked thawed cryoprecipitate units with four bacterial strains: staphylococcus epidermidis, serratia liquefaciens, pseudomonas putida and pseudomonas aeruginosa. no strains grew in the first 4 h of storage, but by 24 h, three of four exhibited up to 1000-fold proliferation. pathogen inactivation t ... | 2017 | 28382701 |
| indole-induced activities of β-lactamase and efflux pump confer ampicillin resistance in pseudomonas putida kt2440. | indole, which is widespread in microbial communities, has received attention because of its effects on bacterial physiology. pseudomonas putida and pseudomonas aeruginosa can acquire ampicillin (amp) resistance during growth on indole-amp agar. transcriptome, mutant, and inhibitor studies have suggested that amp resistance induced by indole can be attributed to increased gene expression of ttgab encoding two genes of rnd-type multidrug efflux operons and an ampc encoding β-lactamase. expression, ... | 2017 | 28352264 |
| dna polymerases imuc and dinb are involved in dna alkylation damage tolerance in pseudomonas aeruginosa and pseudomonas putida. | translesion dna synthesis (tls), facilitated by low-fidelity polymerases, is an important dna damage tolerance mechanism. here, we investigated the role and biological function of tls polymerase imuc (former dnae2), generally present in bacteria lacking dna polymerase v, and tls polymerase dinb in response to dna alkylation damage in pseudomonas aeruginosa and p. putida. we found that tls dna polymerases imuc and dinb ensured a protective role against n- and o-methylation induced by n-methyl-n'- ... | 2017 | 28118378 |
| novel insights into biosynthesis and uptake of rhamnolipids and their precursors. | the human pathogenic bacterium pseudomonas aeruginosa produces rhamnolipids, glycolipids with functions for bacterial motility, biofilm formation, and uptake of hydrophobic substrates. rhamnolipids represent a chemically heterogeneous group of secondary metabolites composed of one or two rhamnose molecules linked to one or mostly two 3-hydroxyfatty acids of various chain lengths. the biosynthetic pathway involves rhamnosyltransferase i encoded by the rhlab operon, which synthesizes 3-(3-hydroxya ... | 2017 | 27988798 |
| expression of the diguanylate cyclase gcba is regulated by fleq in response to cyclic di-gmp in pseudomonas putida kt2440. | cyclic di-gmp (c-di-gmp), a ubiquitous bacterial second messenger that regulates diverse cellular processes, is synthesized by diguanylate cyclase (dgc) and degraded by phosphodiesterase (pde). gcba is a well conserved dgc among pseudomonas species, and has been reported to influence biofilm formation and flagellar motility in pseudomonas fluorescens and pseudomonas aeruginosa. here we confirm the function of gcba in pseudomonas putida and reveal that expression of gcba is regulated by fleq in r ... | 2016 | 27701843 |
| interference competition among household strains of pseudomonas. | bacterial species exhibit biogeographical patterns like those observed in larger organisms. the distribution of bacterial species is driven by environmental selection through abiotic and biotic factors as well dispersal limitations. we asked whether interference competition, a biotic factor, could explain variability in habitat use by pseudomonas species in the human home. to answer this question, we screened almost 8000 directional, pairwise interactions between 89 pseudomonas strains including ... | 2016 | 26276409 |
| effect of rhamnolipid solubilization on hexadecane bioavailability: enhancement or reduction? | in this study, liquid culture systems containing rhamnolipid-solubilized, separate-phase, and multi-state hexadecane as the carbon source were employed for examining the effect of rhamnolipid solubilization on the bioavailability of hexadecane. experimental results showed that the uptake of rhamnolipid-solubilized hexadecane by pseudomonas aeruginosa atcc 9027, a rhamnolipid producing strain, was enhanced compared to the uptake of mass hexadecane as a separate phase, indicating rhamnolipid solub ... | 2017 | 27773441 |
| fleq of pseudomonas putida kt2440 is a multimeric cyclic diguanylate binding protein that differentially regulates expression of biofilm matrix components. | the intracellular signal molecule cyclic di-gmp (c-di-gmp) is an important element in regulation of biofilm formation by bacteria. in pseudomonas aeruginosa, fleq functions as a c-di-gmp-dependent transcriptional regulator of expression of flagellar genes and the exopolysaccharide (eps) pel, a component of the biofilm extracellular matrix. in the plant-beneficial bacterium pseudomonas putida kt2440, a mutation in fleq reduces biofilm formation and colonization of plant surfaces. using isothermal ... | 2017 | 27503246 |
| a novel papillation assay for the identification of genes affecting mutation rate in pseudomonas putida and other pseudomonads. | formation of microcolonies (papillae) permits easy visual screening of mutational events occurring in single colonies of bacteria. in this study, we have established a novel papillation assay employable in a wide range of pseudomonads including pseudomonas aeruginosa and pseudomonas putida for monitoring mutation frequency in distinct colonies. with the aid of this assay, we conducted a genome-wide search for the factors affecting mutation frequency in p. putida. screening ∼27,000 transposon mut ... | 2016 | 27447898 |
| the cryptic dsda gene encodes a functional d-serine dehydratase in pseudomonas aeruginosa pao1. | d-serine, an important neurotransmitter, also contributes to bacterial adaptation and virulence in humans. it was reported that pseudomonas aeruginosa pao1 can grow on d-serine as the sole nitrogen source, and growth was severely reduced in the dada mutant devoid of the d-alanine dehydrogenase with broad substrate specificity. in this study, the dsda gene (pa3357) encoding a putative d-serine dehydratase was subjected to further characterization. growth on d-serine as the sole source of nitrogen ... | 2016 | 26957519 |
| assessment of the contribution of chemoreceptor-based signalling to biofilm formation. | although it is well established that one- and two-component regulatory systems participate in regulating biofilm formation, there also exists evidence suggesting that chemosensory pathways are also involved. however, little information exists about which chemoreceptors and signals modulate this process. here we report the generation of the complete set of chemoreceptor mutants of pseudomonas putida kt2440 and the identification of four mutants with significantly altered biofilm phenotypes. these ... | 2016 | 26662997 |
| removal of 2-butoxyethanol gaseous emissions by biotrickling filtration packed with polyurethane foam. | the removal of 2-butoxyethanol from gaseous emissions was studied using two biotrickling filters (btf1 and btf2) packed with polyurethane foam. two different inoculum sources were used: a pure culture of pseudomonas sp. boe200 (btf1) and activated sludge from a municipal wastewater treatment plant (btf2). the bioreactors were operated at inlet loads (ils) of 130 and 195 g m(-3) hour(-1) and at an empty bed residence time (ebrt) of 12.5s. under an il of ∼130 g m(-3) hour(-1), btf1 presented highe ... | 2016 | 26596886 |
| analysis of preference for carbon source utilization among three strains of aromatic compounds degrading pseudomonas. | soil isolates pseudomonas putida csv86, pseudomonas aeruginosa pp4 and pseudomonas sp. c5pp degrade naphthalene, phthalate isomers and carbaryl, respectively. strain csv86 displayed a diauxic growth pattern on phenylpropanoid compounds (veratraldehyde, ferulic acid, vanillin or vanillic acid) plus glucose with a distinct second lag-phase. the glucose concentration in the medium remained constant with higher cell respiration rates on aromatics and maximum protocatechuate 3,4-dioxygenase activity ... | 2015 | 26316546 |
| genome-scale reconstruction of the metabolic network in pseudomonas stutzeri a1501. | pseudomonas stutzeri a1501 is an endophytic bacterium capable of nitrogen fixation. this strain has been isolated from the rice rhizosphere and provides the plant with fixed nitrogen and phytohormones. these interesting features encouraged us to study the metabolism of this microorganism at the systems-level. in this work, we present the first genome-scale metabolic model (ipb890) for p. stutzeri, involving 890 genes, 1135 reactions, and 813 metabolites. a combination of automatic and manual app ... | 2015 | 26302703 |
| analysis of the core genome and pangenome of pseudomonas putida. | pseudomonas putida are strict aerobes that proliferate in a range of temperate niches and are of interest for environmental applications due to their capacity to degrade pollutants and ability to promote plant growth. furthermore solvent-tolerant strains are useful for biosynthesis of added-value chemicals. we present a comprehensive comparative analysis of nine strains and the first characterization of the pseudomonas putida pangenome. the core genome of p. putida comprises approximately 3386 g ... | 2016 | 26261031 |
| isolation of carbapenem-resistant pseudomonas spp. from food. | pseudomonas spp. are ubiquitous in nature. carbapenem resistance in environmental isolates of members of this genus is thought to be rare but the exact resistance rate is unknown. in this study, carbapenem-resistant pseudomonas spp. were isolated from chicken and pork samples and the mechanisms underlying the carbapenem resistance in these strains were investigated. a total of 16 carbapenem-resistant pseudomonas aeruginosa, pseudomonas putida and pseudomonas otitidis isolates were recovered from ... | 2015 | 27873658 |
| specific gamma-aminobutyrate chemotaxis in pseudomonads with different lifestyle. | the pctc chemoreceptor of pseudomonas aeruginosa mediates chemotaxis with high specificity to gamma-aminobutyric acid (gaba). this compound is present everywhere in nature and has multiple functions, including being a human neurotransmitter or plant signaling compound. because p. aeruginosa is ubiquitously distributed in nature and able to infect and colonize different hosts, the physiological relevance of gaba taxis is unclear, but it has been suggested that bacterial attraction to neurotransmi ... | 2015 | 25921834 |
| diversity of small rnas expressed in pseudomonas species. | rna sequencing (rna-seq) has revealed several hundreds of previously undetected small rnas (srnas) in all bacterial species investigated, including strains of pseudomonas aeruginosa, pseudomonas putida and pseudomonas syringae. nonetheless, only little is known about the extent of conservation of expressed srnas across strains and species. in this study, we have used rna-seq to identify srnas in p. putida dot-t1e and pseudomonas extremaustralis 14-3b. this is the first strain of p. extremaustral ... | 2015 | 25394275 |
| the c-di-gmp phosphodiesterase bifa is involved in the virulence of bacteria from the pseudomonas syringae complex. | in a recent screen for novel virulence factors involved in the interaction between pseudomonas savastanoi pv. savastanoi and the olive tree, a mutant was selected that contained a transposon insertion in a putative cyclic diguanylate (c-di-gmp) phosphodiesterase-encoding gene. this gene displayed high similarity to bifa of pseudomonas aeruginosa and pseudomonas putida. here, we examined the role of bifa in free-living and virulence-related phenotypes of two bacterial plant pathogens in the pseud ... | 2015 | 25385023 |
| the pseudomonas community in metal-contaminated sediments as revealed by quantitative pcr: a link with metal bioavailability. | pseudomonas bacteria are ubiquitous gram-negative and aerobic microorganisms that are known to harbor metal resistance mechanisms such as efflux pumps and intracellular redox enzymes. specific pseudomonas bacteria have been quantified in some metal-contaminated environments, but the entire pseudomonas population has been poorly investigated under these conditions, and the link with metal bioavailability was not previously examined. in the present study, quantitative pcr and cell cultivation were ... | 2014 | 25102022 |
| pseudomonas aeruginosa mdab and wrba are water-soluble two-electron quinone oxidoreductases with the potential to defend against oxidative stress. | water-soluble quinone oxidoreductases capable of reducing quinone substrates via a concerted two-electron mechanism have been implicated in bacterial antioxidant defence. twoelectron transfer avoids formation of dangerously reactive semi-quinone intermediates, moreover previous work in pseudomonas putida indicated a direct protective effect for the quinols generated by an over-expressed oxidoreductase. here, the pseudomonas aeruginosa orthologs of five quinone oxidoreductases--mdab, chrr, wrba, ... | 2014 | 25085734 |
| hierarchical management of carbon sources is regulated similarly by the cbra/b systems in pseudomonas aeruginosa and pseudomonas putida. | the cbra/b system in pseudomonads is involved in the utilization of carbon sources and carbon catabolite repression (ccr) through the activation of the small rnas crcz in pseudomonas aeruginosa, and crcz and crcy in pseudomonas putida. interestingly, previous works reported that the cbra/b system activity in p. aeruginosa pao1 and p. putida kt2442 responded differently to the presence of different carbon sources, thus raising the question of the exact nature of the signal(s) detected by cbra. he ... | 2014 | 25031426 |
| a model-based approach to detect interspecific interactions during biofilm development. | a model-based approach was developed to detect interspecific interactions during biofilm development. this approach relied on the comparison of experimental data with a simple null model of biofilm growth dynamics where individual species grew independently of one another, except that they competed for space. such a model was directly parameterized with a 4d confocal image series of biofilms and then used as a null model to detect interspecific interactions between pairs of bacterial species. th ... | 2014 | 24963685 |
| fusion protein bilayer fabrication composed of recombinant azurin/cytochrome p450 by the sortase-mediated ligation method. | recently, the fabrication of protein bilayer has been required for the development of protein or enzyme complex formation. in the present study, we fabricated a fusion protein bilayer composed of recombinant azurin-cytochrome p450, which was synthesized by a site-specific sortase-mediated ligation method. the pseudomonas aeruginosa azurin was modified by dna recombinant technique, for enzymatic ligation and immobilization. the pseudomonas putida cytochrome p450 was also modified for enzymatic li ... | 2014 | 24924834 |
| evaluation of nosocomial blood stream infections caused by pseudomonas species in newborns. | nosocomial blood stream infections (nbsi) are a significant hazard in health care facilities, exacting a tremendous toll and causing increased morbidity, mortality, and increased length of hospital stay and health care costs. | 2014 | 24779295 |
| a role for the regulator psra in the polyhydroxyalkanoate metabolism of pseudomonas putida kt2440. | pseudomonas putida kt2440 is a gram-negative bacterium capable of producing medium-chain-length-polyhydroxyalkanoates (mcl-pha). when fatty acids are used as growth and polymer precursors, the biosynthesis is linked to fatty acid metabolism via ß-oxidation route. in the close-related pseudomonas aeruginosa, the transcriptional repressor psra regulates the ß-oxidation, but little is known about the regulatory system in p. putida. to analyze the effect of the absence of psra gene on the growth and ... | 2014 | 24751507 |
| selective reactivity of monochloramine with extracellular matrix components affects the disinfection of biofilm and detached clusters. | the efficiency of monochloramine disinfection was dependent on the quantity and composition of extracellular polymeric substances (eps) in biofilms, as monochloramine has a selective reactivity with proteins over polysaccharides. biofilms with protein-based (pseudomonas putida) and polysaccharide based eps (pseudomonas aeruginosa), as well as biofilms with varied amount of polysaccharide eps (wild-type and mutant p. aeruginosa), were compared. the different reactivity of eps components with mono ... | 2014 | 24575887 |
| biotransformation of trinitrotoluene (tnt) by pseudomonas spp. isolated from a tnt-contaminated environment. | the compound 2,4,6-trinitrotoluene (tnt) is a secondary explosive widely used worldwide for both military and civil purposes. as a result, residual tnt has been detected as an environmental pollutant in both soil and groundwater. the authors have isolated several microbial strains from soil contaminated with tnt by enrichment culture techniques using tnt as a carbon, nitrogen, and energy source. the contaminated soil contained approximately 1860 ppm tnt measured by high-performance liquid chroma ... | 2014 | 24549634 |
| expression, purification, and characterization of formaldehyde dehydrogenase from pseudomonas aeruginosa. | as a member of zinc-containing medium-chain alcohol dehydrogenase family, formaldehyde dehydrogenase (fdh) can oxidize toxic formaldehyde to less active formate with nad(+) as a cofactor and exists in both prokaryotes and eukaryotes. most fdhs are well known to be glutathione-dependent in the catalysis of formaldehyde oxidation, but the enzyme from pseudomonas putida is an exception, which is independent of glutathione. to identify novel glutathione-independent fdhs from other bacterial strains ... | 2013 | 24125754 |
| [isolation, identification of two aerobic denitrifiers and bioaugmentation for enhancing denitrificaition of biofilm under oligotrophic conditions]. | two aerobic denitrifying bacteria were isolated from the sludge of municipal wastewater treatment plant by enrichment, preliminary screening with btb culture medium and the denitrification potential test and identified through 16s rdna sequence analysis. then they were bioaugmented to oligotrophic biofilm system respectively, aiming to enhance the denitrification capacity. the two strains were identified as pseudomonas aeruginosa and pseudomonas putida respectively. they could remove 78% or 82% ... | 2013 | 24028024 |
| statistical screening of medium components for recombinant production of pseudomonas aeruginosa atcc 9027 rhamnolipids by nonpathogenic cell factory pseudomonas putida kt2440. | rhamnolipids (rls) produced by the opportunistic human pathogen pseudomonas aeruginosa are considered as potential candidates for the next generation of surfactants. large-scale production of rls depends on progress in strain engineering, medium design, operating strategies, and purification procedures. in this work, the rhlab genes extracted from a mono_rls_producing strain of p. aeruginosa (atcc 9027) were introduced to an appropriate safety host pseudomonas putida kt2440. the capability of th ... | 2014 | 23943464 |
| [the experience of applying techniques of molecular genetics in identification of clinical strains pseudomonas aeruginosa]. | the article presents comparative analysis of application of common bacteriologic and molecular techniques to identify p. aerugenosa. the genetic detection was applied using polymerase chain reaction with genus-specific and species-specific primers and amplification of conservative sites of gens 16s prna with successive identification of bacteria by sequenation. it is established that 95% (151) of strains correspond to species of p. aerugenosa detected in primary bacteriologic laboratories and on ... | 2013 | 23808024 |
| first detection in europe of the metallo-β-lactamase imp-15 in clinical strains of pseudomonas putida and pseudomonas aeruginosa. | in a prospective study (2009-2011) in healthcare institutions from the canary islands (spain), 6 out of 298 carbapenem non-susceptible pseudomonas aeruginosa isolates produced a metallo-β-lactamase: four imp-15, two vim-2 (including one imp-15-positive isolate) and one vim-1. multilocus sequence typing identified the single vim-1-producing isolate as clone st111 and two imp-15-producing isolates as st606, but, strikingly, bacterial re-identification revealed that the other three isolates (produc ... | 2013 | 23656535 |
| a pseudomonas putida bioreporter for the detection of enzymes active on 2-alkyl-4(1h)-quinolone signalling molecules. | the quorum sensing signalling molecules 2-heptyl-3-hydroxy-4(1h)-quinolone, termed the "pseudomonas quinolone signal" (pqs), and 2-heptyl-4(1h)-quinolone (hhq) play an important role in the control of virulence gene expression in pseudomonas aeruginosa. to construct a bioreporter for the specific and sensitive detection of these compounds, a plasmid with the pqsr gene encoding the pqs- and hhq-responsive transcriptional regulator pqsr, and with the pqsr-controlled pqsa promoter fused to the lacz ... | 2013 | 22740050 |
| identification of reciprocal adhesion genes in pathogenic and non-pathogenic pseudomonas. | we used a combination of in silico and large-scale mutagenesis approaches to expand our current knowledge of the genetic determinants used by pseudomonas putida kt2440 to attach to surfaces. we first identified in silico orthologues that have been annotated in pseudomonas aeruginosa as potentially involved in attachment. in this search 67 paired-related genes of p. putida kt2440 and p. aeruginosa were identified as associated to adhesion. to test the potential role of the corresponding gene prod ... | 2013 | 22458445 |
| caveat emptor: the role of suboptimal bronchoscope repair practices by a third-party vendor in a pseudo-outbreak of pseudomonas in bronchoalveolar lavage specimens. | to describe a pseudo-outbreak associated with loose bronchoscope biopsy ports caused by inadequate bronchoscope repair practices by third-party vendors and to alert healthcare personnel to assess bronchoscope repair practices. | 2012 | 22314057 |
| a simple strategy for investigating the diversity and hydrocarbon degradation abilities of cultivable bacteria from contaminated soil. | the use of indigenous bacterial strains is a valuable bioremediation strategy for cleaning the environment from hydrocarbon pollutants. the isolation and selection of hydrocarbon-degrading bacteria is therefore crucial for obtaining the most promising strains for site decontamination. two different media, a minimal medium supplemented with a mixture of polycyclic aromatic hydrocarbons and a ms medium supplemented with triphenyltetrazolium chloride, were used for the isolation of bacterial strain ... | 2013 | 23386320 |
| [isolation, identification and characterization of five pseudomonas strains]. | we isolated pseudomonas strains from soil samples collected from changsha, hunan province, catalogued them and studied the antimicrobial and antitumor activity. | 2013 | 24377248 |
| does doping with aluminum alter the effects of zno nanoparticles on the metabolism of soil pseudomonads? | doping of zno nanoparticles (nps) is being used to increase their commercialization in the optical and semiconductor fields. this paper addresses whether doping with al alters how zno nps at nonlethal levels modifies the metabolism of soil-borne pseudomonads which are beneficial in performing bioremediation or promoting plant growth. the differences in x-ray diffraction (xrd) patterns, observed between commercial zno and al-doped zno nps indicated the aluminum was present as al nps. both particl ... | 2013 | 23083747 |
| pseudomonas soli sp. nov., a novel producer of xantholysin congeners. | a chemoorganotrophic gram-negative bacterium was isolated by means of a diffusion sandwich system from a soil sample from the sierra nevada national park, spain. strain f-279,208(t) was oxidase and catalase positive, strictly aerobic, non-spore-forming and motile by single polar flagellum. phylogenetic analysis of the 16s rrna, gyrb, rpob and rpod genes revealed that strain f-279,208(t) belongs to the pseudomonas putida group with pseudomonas mosselii and pseudomonas entomophila as its closest r ... | 2014 | 25097020 |
| engineering the diversity of polyesters. | many bacteria have been found to produce various polyhydroxyalkanoates (pha) biopolyesters. in many cases, it is not easy to control the structures of pha including homopolymers, random copolymers and block copolymers as well as ratios of monomers in the copolymers. it has become possible to engineer bacteria for controllable synthesis of pha with the desirable structures by creating new pha synthesis pathways. remarkably, the weakening of β-oxidation cycle in pseudomonas putida and pseudomonas ... | 2014 | 24632193 |
| taxonomic characterisation of pseudomonas strain l48 and formal proposal of pseudomonas entomophila sp. nov. | an entomopathogenic, gram-negative bacterium isolated from a female specimen of the fruit fly drosophila melanogaster was taxonomically characterised. strain l48(t) was strictly aerobic, non-fermentative, oxidase and catalase positive, rod-shaped, and motile due to a polar inserted flagellum. phylogenetic analysis of the 16s rrna gene and three other housekeeping genes placed strain l48 (t) in the pseudomonas putida phylogenetic group. dna-dna hybridisation studies together with phenotypic metab ... | 2012 | 22326814 |
| antifreeze protein activity in arctic cryoconite bacteria. | fourteen arctic bacterial strains belonging to five genera, cryobacterium, leifsonia, polaromonas, pseudomonas, and subtercola isolated from sediments found in cryoconite holes of arctic glaciers, were subjected to screening for antifreeze proteins (afps). eight strains showed afp activity, and six strains of four species were further characterized. pseudomonas ficuserectae exhibited a high thermal hysteresis (th) activity. ice recrystallization inhibition (iri) activity was observed in most cul ... | 2014 | 24283367 |
| two-step bioleaching of copper and gold from discarded printed circuit boards (pcb). | an effective strategy for environmentally sound biological recovery of copper and gold from discarded printed circuit boards (pcb) in a two-step bioleaching process was experimented. in the first step, chemolithotrophic acidophilic acidithiobacillus ferrivorans and acidithiobacillus thiooxidans were used. in the second step, cyanide-producing heterotrophic pseudomonas fluorescens and pseudomonas putida were used. results showed that at a 1% pulp density (10g/l pcb concentration), 98.4% of the co ... | 2016 | 26704063 |
| hydrogen isotope fractionation as a tool to identify aerobic and anaerobic pah biodegradation. | aerobic and anaerobic polycyclic aromatic hydrocarbon (pah) biodegradation was characterized by compound specific stable isotope analysis (csia) of the carbon and hydrogen isotope effects of the enzymatic reactions initiating specific degradation pathways, using naphthalene and 2-methylnaphtalene as model compounds. aerobic activation of naphthalene and 2-methylnaphthalene by pseudomonas putida ncib 9816 and pseudomonas fluorescens atcc 17483 containing naphthalene dioxygenases was associated wi ... | 2016 | 26855125 |
| evaluation of phosphate-solubilizing bacteria on the growth and grain yield of rice (oryza sativa l.) cropped in northern iran. | this study aimed to evaluate the efficiency of four phosphate-solubilizing bacteria (psb) on the growth and yield of rice under different soil conditions. | 2015 | 26294004 |
| structural characterization of multiple pyoverdines secreted by two pseudomonas strains using liquid chromatography-high resolution tandem mass spectrometry with varying dissociation energies. | high-affinity iron (fe)-scavenging molecules, or siderophores, are secreted by microorganisms to acquire and compete for fe. pyoverdine (pvd), the primary siderophore produced by pseudomonas, consists of a dihydroxyquinoline-type chromophore, a peptide chain of variable length and conformation, and a side chain composed of a dicarboxylic acid or its monoamide derivative. elucidation of the pvd structures secreted by different pseudomonas strains is an important step toward understanding their fe ... | 2015 | 25895945 |
| pseudomonads biodegradation of aromatic compounds in oil sands process-affected water. | aromatic naphthenic acids (nas) have been shown to be more toxic than the classical nas found in oil sands process-affected water (ospw). to reduce this toxicity, pseudomonas fluorescens and pseudomonas putida were used to determine their ability to biodegrade aromatic compounds including treatments considering the impacts of external carbon and iron addition. results showed that with added carbon p. fluorescens and p. putida have the capability of biodegrading these aromatics. in the presence o ... | 2015 | 25828413 |
| comparison of biomass detachment from biofilms of two different pseudomonas spp. under constant shear conditions. | in the context of biofilm development, detachment is of practical importance when placed in a biofilm management perspective. the objective of the present study was to examine biofilm structure and biofilm detachment under controlled conditions for two distinct microorganisms grown under constant shear conditions. detached biofilm biomass was regularly collected and analysed over the course of 72 h biofilm growth by pseudomonas putida and pseudomonas fluorescens cells, and biofilm structural dev ... | 2015 | 25563340 |
| characterization of the ability to form biofilms by plant-associated pseudomonas species. | successful colonization is the initial step for plant-bacteria interactions; therefore, the development of strategies to improve adherence to plant surfaces is critically important for environmental bacteria. biofilm formation is thought to be one such strategy for bacteria to establish stable colonization on inert and living surfaces. although biofilms play potential roles in enabling persistent bacterial colonization, little attention has been paid to biofilms formed by plant-associated bacter ... | 2015 | 25487118 |
| bacterial adhesion onto nanofiltration and reverse osmosis membranes: effect of permeate flux. | the influence of permeate flux on bacterial adhesion to nf and ro membranes was examined using two model pseudomonas species, namely pseudomonas fluorescens and pseudomonas putida. to better understand the initial biofouling profile during nf/ro processes, deposition experiments were conducted in cross flow under permeate flux varying from 0.5 up to 120 l/(h m(2)), using six nf and ro membranes each having different surface properties. all experiments were performed at a reynolds number of 579. ... | 2014 | 25016321 |
| cell system engineering to produce extracellular polyhydroxyalkanoate depolymerase with targeted applications. | novel platforms based on the application of bacterial cell systems as factories for production of new bioproducts open avenues and dramatically expand the catalogue of existing biomaterials. herein, we designed the strategy based on in vivo production of extracellular pseudomonas fluorescens gk13 (phazgk13) depolymerase to degrade previously biosynthesized polyhydroxyalkanotes (phas) or to obtain 3-hydroxyalkanoic acids (has). with this aim, extracellular phazgk13 was produced in recombinant str ... | 2014 | 24751505 |
| combined inoculation of pseudomonas fluorescens and trichoderma harzianum for enhancing plant growth of vanilla (vanilla planifolia). | this study was conducted to evaluate the plant growth promoting efficiency of combined inoculation of rhizobacteria on vanilla plants. based on the in vitro performance of indigenous trichoderma spp. and pseudomonas spp., four effective antagonists were selected and screened under greenhouse experiment for their growth enhancement potential. the maximum percentage of growth enhancement were observed in the combination of trichoderma harzianum with pseudomonas fluorescens treatment followed by ps ... | 2013 | 24494528 |
| the effect of soil bioaugmentation with strains of pseudomonas on cd, zn and cu uptake by sinapis alba l. | the aim of this study was to assess the ability of selected metal resistant strains of the pseudomonas genus to increase zn, cd and cu uptake by the metalophyte sinapis alba l. under laboratory conditions. moreover, the mechanisms of the plant growth promotion in the tested strains and their impact on the shoots and roots of white mustard biomass were examined. soil inoculation with the tested strains resulted in higher concentrations of zn, cd and cu in the shoots and roots of the plants in com ... | 2013 | 23561856 |
| phylogenetic affiliation of pseudomonas putida biovar a and b strains. | in the genus pseudomonas, two main lineages and 19 phylogenetic groups or subgroups have been defined based on dna sequence studies of 16s rrna, gyrb and rpod genes. in the present study, 33 strains previously classified as pseudomonas putida were phylogenetically affiliated with their closest relatives in the genus by multilocus sequence analysis. the results demonstrated that strains assigned to biovar a of the species were located in the p. putida group, though not all belonged to the species ... | 2013 | 23391610 |
| delay of flower senescence by bacterial endophytes expressing 1-aminocyclopropane-1-carboxylate deaminase. | the ability of 1-aminocyclopropane-1-carboxylate (acc) deaminase-containing plant growth-promoting bacterial (pgpb) endophytes pseudomonas fluorescens yss6 and pseudomonas migulae 8r6, their acc deaminase minus mutants and the rhizospheric plant growth-promoting bacterium pseudomonas putida uw4 to delay the senescence of mini carnation cut flowers was assessed. | 2012 | 22816486 |
| the effect of a competitor on a model adaptive radiation. | the ecological forces shaping adaptive radiations are of great interest to evolutionary ecologists. here, we experimentally test the hypothesis that the diversification of a lineage should be limited in the presence of competition from another taxon. we do this by studying a model microbial adaptive radiation (the generation of phenotypic diversity in asexual lineages of the bacterium pseudomonas fluorescens) in the presence or absence of a competitor (pseudomonas putida). in a spatially heterog ... | 2012 | 22671562 |
| description of pseudomonas jessenii subsp. pseudoputida subsp. nov., amended description of pseudomonas jessenii and description of pseudomonas jessenii subsp. jessenii subsp. nov. | fluorescent pseudomonas putida ccm 3656 (atcc 11250) was analysed according to the methods of polyphasic approach which were based on sequence analyses involving the rpob and rrs genes, manual ribotyping using endonuclease hindiii, dna base composition determination and dna-dna hybridization. the results obtained by these genotyping methods showed that the strain ccm 3656 is distant from p. putida taxon, which was supported with phenotype characterization represented by whole-cell protein profil ... | 2013 | 23636777 |
| isolation and characteristics of sulfentrazone-degrading bacteria. | this study aimed to isolate and characterize bacteria able to use sulfentrazone in the commercial formulation as their sole carbon source. the isolation of the potential sulfentrazone-degrading bacteria was made from soil samples with a recent history of herbicide application and from isolates identified through rdna sequencing. subsequently, we assessed the growth of the isolates and their sulfentrazone degradation ability using high-performance liquid chromatography. twenty-six potential sulfe ... | 2017 | 27820678 |
| isolation and characterization of bacteria that use furans as the sole carbon source. | five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. based on 16s rrna phylogenetic analysis, these strains were identified as cupriavidus pinatubonensis (designated als1280), pigmentiphaga sp. (als1172), pseudomonas sp. bwdy (als1279), pseudomonas mendocina (als1131), and pseudomonas putida (als1267). in all cases, growth under oxygenated conditions on furfural was accompanied by the transient accumulation of 2-furo ... | 2016 | 26419660 |
| an upp-based markerless gene replacement method for genome reduction and metabolic pathway engineering in pseudomonas mendocina nk-01 and pseudomonas putida kt2440. | a markerless gene replacement method was adapted by combining a suicide plasmid, pex18tc, with a counterselectable marker, the upp gene encoding uracil phosphoribosyltransferase (uprtase), for the medium-chain length polyhydroxyalkanoates (pha(mcl))-producing strain pseudomonas mendocina nk-01. an nk-01 5-fluorouracil (5-fu) resistant background strain was first constructed by deleting the chromosomal upp gene. the suicide plasmid pex18tc, carrying a functional allele of the upp gene of p. mendo ... | 2015 | 25828098 |
| pseudomonas hunanensis sp. nov., isolated from soil subjected to long-term manganese pollution. | a gram-negative, polar flagella, rod-shaped bacterium lv (t) was isolated from a soil sample subjected to long-term manganese pollution in hunan province, china. cells grow optimally on luria-bertani agar medium at 30 °c in the presence of 0-5.0 % (w/v) nacl and ph 7-8. 16s rrna gene sequence analysis revealed that strain lv (t) belonged to the genus pseudomonas, with sequence similarity values of 98.6, 98.2, 98.7, and 97.3 % to pseudomonas monteilii bcrc 17520 (t) , pseudomonas putida bcrc 1045 ... | 2014 | 24562759 |
| production and characterization of medium-chain-length polyhydroxyalkanoates by pseudomonas mosselii to7. | the polyhydroxyalkanoate (pha) production and growth of pseudomonas mosselii to7, a newly isolated pseudomonas species from the wastewater of a vegetable oil manufacturing facility, was analyzed. phenotypic analysis and phylogenetic analysis of the 16s rrna gene revealed that it is closely related to pseudomonas mosselii. in the presence of palm kernel and soybean oils, p. mosselii to7 produced up to 50% cell dry weight (cdw) medium-chain-length (mcl) phas comprising high poly(3-hydroxyoctanoate ... | 2014 | 24630613 |
| switching a newly discovered lactonase into an efficient and thermostable phosphotriesterase by simple double mutations his250ile/ile263trp. | ophc2 is a thermostable organophosphate (op) hydrolase in the β-lactamase superfamily. ops are highly toxic synthetic chemicals with no natural analogs. how did ophc2 acquire phosphotriesterase (pte) activity remained unclear. in this study, an ophc2 analogue, pooph was discovered from pseudomonas oleovorans exhibiting high lactonase and esterase activities and latent pte activity. sequence analysis revealed conserved his250 and ile263 and site-directed mutagenesis at these crucial residues enha ... | 2014 | 24771278 |
| mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens. | there is a paucity of data regarding the microbial constituents of tobacco products and their impacts on public health. moreover, there has been no comparative characterization performed on the bacterial microbiota associated with the addition of menthol, an additive that has been used by tobacco manufacturers for nearly a century. to address this knowledge gap, we conducted bacterial community profiling on tobacco from user- and custom-mentholated/non-mentholated cigarette pairs, as well as a c ... | 2017 | 28202080 |
| highly efficient simultaneous adsorption and biodegradation of a highly-concentrated anionic dye by a high-surface-area carbon-based biocomposite. | mesoporous high-surface-area activated carbon (mhsa-ac), which has a honeycomb structure, was produced from coconut shells by simultaneous chemical and physical activation and used for the rapid adsorption of an anionic dye, namely acid orange 10 (ao10), from water. owing to its porosity and high brunauer-emmett-teller surface area (2283.91 m(2)g(-1)), mhsa-ac is a highly efficient adsorbent. it also has good biocompatibility and is a good immobilization carrier; the grooves on the mhsa-ac surfa ... | 2017 | 28365499 |
| draft genome sequence of pseudomonas putida strain gm4fr, an endophytic bacterium isolated from festuca rubra l. | pseudomonas putida gm4fr is an endophytic bacterium isolated from aerial plant tissues of festuca rubra l. functional annotation of the draft genome (7.1 mb) revealed 6,272 predicted protein-encoding genes. the genome provides insights into the biocontrol and plant growth-promoting potential of p. putida gm4fr. | 2017 | 28360162 |
| deconvolution of gene expression noise into spatial dynamics of transcription factor-promoter interplay. | gene expression noise is not only the mere consequence of stochasticity, but also a signal that reflects the upstream physical dynamics of the cognate molecular machinery. soil bacteria facing recalcitrant pollutants exploit noise of catabolic promoters to deploy beneficial phenotypes such as metabolic bet-hedging and/or division of biochemical labour. although the role of upstream promoter-regulator interplay in the origin of this noise is little understood, its specifications are probably ciph ... | 2017 | 28355056 |
| use of response surface method for maximizing the production of arginine deiminase by pseudomonas putida. | statistically designed experiments were used to optimize the production of arginine deiminase (adi) by pseudomonas putida kt2440 in batch culture. a plackett-burman design involving eleven factors showed that adi production was most influenced by the initial ph and the initial concentrations of glucose and yeast extract. a central composite experimental design showed that the optimal values of these factors were 8.0, 10 g/l and 12.5 g/l, respectively. the other components of the optimal culture ... | 2016 | 28352521 |
| metabolic engineering to expand the substrate spectrum of pseudomonas putida toward sucrose. | sucrose is an important disaccharide used as a substrate in many industrial applications. it is a major component of molasses, a cheap by-product of the sugar industry. unfortunately, not all industrially relevant organisms, among them pseudomonas putida, are capable of metabolizing sucrose. we chose a metabolic engineering approach to circumvent this blockage and equip p. putida with the activities necessary to consume sucrose. therefore, we constructed a pair of broad-host range mini-transposo ... | 2017 | 28349670 |
| the impact of succinate trace on pww0 and ortho-cleavage pathway transcription in pseudomonas putida mt-2 during toluene biodegradation. | toluene is a pollutant catabolised through the interconnected pww0 (tol) and ortho-cleavage pathways of pseudomonas putida mt-2, while upon succinate and toluene mixtures introduction in batch cultures grown on m9 medium, succinate was previously reported as non-repressing. the effect of a 40 times lower succinate concentration, as compared to literature values, was explored through systematic real-time qpcr monitoring of transcriptional kinetics of the key tol pu, pm and ortho-cleavage pbenr, p ... | 2017 | 28347959 |
| toxicity testing of pristine and aged silver nanoparticles in real wastewaters using bioluminescent pseudomonas putida. | impact of aging on nanoparticle toxicity in real matrices is scarcely investigated due to a lack of suitable methodologies. herein, the toxicity of pristine and aged silver nanoparticles (ag nps) to a bioluminescent pseudomonas putida bioreporter was measured in spiked crude and final wastewater samples (cws and fws, respectively) collected from four wastewater treatment plants (wwtps). results showed lower toxicity of pristine ag nps in cws than in fws. the effect of the matrix on the eventual ... | 2016 | 28344306 |
| structure and mechanism of benzaldehyde dehydrogenase from pseudomonas putida atcc 12633, a member of the class 3 aldehyde dehydrogenase superfamily. | benzaldehyde dehydrogenase from pseudomonas putida (ppbadh) belongs to the class 3 aldehyde dehydrogenase (aldh) family. the class 3 aldhs are unusual in that they are generally dimeric (rather than tetrameric), relatively non-specific and utilize both nad+ and nadp+. to date, x-ray structures of three class 3 aldhs have been determined, of which only two have cofactor bound, both in the nad+ form. here we report the crystal structure of ppbadh in complex with nadp+ and a thioacyl intermediate a ... | 2017 | 28338942 |
| structural and mechanistic insights into homocysteine degradation by a mutant of methionine γ-lyase based on substrate-assisted catalysis. | methionine γ-lyse (mgl) catalyzes the α, γ-elimination of l-methionine and its derivatives as well as the α, β-elimination of l-cysteine and its derivatives to produce α-keto acids, volatile thiols, and ammonia. the reaction mechanism of mgl has been characterized by enzymological studies using several site-directed mutants. the pseudomonas putida mgl c116h mutant showed drastically reduced degradation activity toward methionine while retaining activity toward homocysteine. to understand the und ... | 2017 | 28329912 |
| osmotic stress in colony and planktonic cells of pseudomonas putida mt-2 revealed significant differences in adaptive response mechanisms. | planktonic cells and those grown on surfaces (or as colony biofilm) are known to show significant differences regarding growth behavior, cell physiology, gene expression and stress tolerance. in order to compare stress behavior of different growth forms, shake cultures for planktonic growth and agar plate cultivation for colony growth, were carried out with the well investigated model organism, pseudomonas putida mt-2. cells were exposed to sodium chloride to cause osmotic stress as one main env ... | 2017 | 28299749 |
| conversion of lignin model compounds by pseudomonas putida kt2440 and isolates from compost. | starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16s ribosomal rna identification of the isolates showed high score similarity with pseudomonas spp. for three out of four isolates. further characterization of growth on mixtures of six selected lignin model compounds (vanillin, vanillate, 4-hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the pseudomonas isolates and in addition with the strain pseudomonas ... | 2017 | 28299400 |
| experimental validation of in silico estimated biomass yields of pseudomonas putida kt2440. | pseudomonas putida is rapidly becoming a microbial cell platform for biotechnological applications. in order to understand genotype-phenotype relationships genome scale models represent helpful tools. however, the validation of in silico predictions of genome scale models is a task that is rarely performed. in this study the theoretical biomass yields of pseudomonas putida kt2440 were estimated for 57 different carbon sources based on a genome scale stoichiometric model applying flux balance ana ... | 2017 | 28294579 |
| nitrogen removal characteristics of pseudomonas putida y-9 capable of heterotrophic nitrification and aerobic denitrification at low temperature. | the cold-adapted bacterium pseudomonas putida y-9 was investigated and exhibited excellent capability for nitrogen removal at 15°c. the strain capable of heterotrophic nitrification and aerobic denitrification could efficiently remove ammonium, nitrate, and nitrite at an average removal rate of 2.85 mg, 1.60 mg, and 1.83 mg nl(-1 )h(-1), respectively. strain y-9 performed nitrification in preference to denitrification when ammonium and nitrate or ammonium and nitrite coexisted in the solution. m ... | 2017 | 28293626 |
| bacterial effects and interfacial inactivation mechanism of nzvi/pd on pseudomonas putida strain. | with the introduction of nano zero valent iron (nzvi) technology into our environment, its potential environmental risk to environmental microorganisms has attracted considerable attention. in this study, pseudomonas putida was chosen as a typical strain to study the bacterial toxicity of nzvi/pd. the cfu assay results indicated that nzvi/pd was toxic to p. putida cells but the toxicity decreased with an increase in do. the experiments isolated by dialysis bag and flow cytometry analysis suggest ... | 2017 | 28285239 |
| production of polyhydroxyalkanoates from sludge palm oil using pseudomonas putida s12. | polyhydroxyalkanoates (phas) are biodegradable plastics produced by bacteria, but their use in diverse applications is prohibited by high production costs. to reduce these costs, the conversion of phas from crude sludge palm oil (spo) as an inexpensive renewable raw material by pseudomonas strains was tested. pseudomonasputida s12 was found to produce the highest yield (~41%) of elastomeric medium-chain-length (mcl)-phas from spo. the mcl-pha characteristics were analyzed by gas-chromatography/m ... | 2017 | 28274100 |
| cooperation in carbon source degradation shapes spatial self-organization of microbial consortia on hydrated surfaces. | mounting evidence suggests that natural microbial communities exhibit a high level of spatial organization at the micrometric scale that facilitate ecological interactions and support biogeochemical cycles. microbial patterns are difficult to study definitively in natural environments due to complex biodiversity, observability and variable physicochemical factors. here, we examine how trophic dependencies give rise to self-organized spatial patterns of a well-defined bacterial consortium grown o ... | 2017 | 28262696 |
| ultrasonic disruption of pseudomonas putida for the release of arginine deiminase: kinetics and predictive models. | the responses of the ultrasound-mediated disruption of pseudomonas putida kt2440 were modelled as the function of biomass concentration in the cell suspension; the treatment time of sonication; the duty cycle and the acoustic power of the sonicator. for the experimental data, the response surface (rsm), the artificial neural network (ann) and the support vector machine (svm) models were compared for their ability to predict the performance parameters. the satisfactory prediction of the unseen da ... | 2017 | 28260664 |
| high performance and prospective application of xanthate-modified thiourea chitosan sponge-combined pseudomonas putida and talaromyces amestolkiae biomass for pb(ii) removal from wastewater. | biosorption using microbes has been proved to be an efficient technology to remove heavy metals from wastewater, whereas the imperfections in mechanical property and separation limit their practical application. in this study, pseudomonas putida i3 and talaromyces amestolkiae pb respectively combined with xanthate-modified thiourea chitosan sponge (pxtcs and txtcs) were synthesized to investigate the pb(ii) removal ability from solutions. the prepared biosorbents possessed a three-dimensional ma ... | 2017 | 28258997 |
| potential microbial contamination during sampling of permafrost soil assessed by tracers. | drilling and handling of permanently frozen soil cores without microbial contamination is of concern because contamination e.g. from the active layer above may lead to incorrect interpretation of results in experiments investigating potential and actual microbial activity in these low microbial biomass environments. here, we present an example of how microbial contamination from active layer soil affected analysis of the potentially active microbial community in permafrost soil. we also present ... | 2017 | 28230151 |
| a facile approach for surface alteration of pseudomonas putida i3 by supplying k2so4 into growth medium: enhanced removal of pb(ii) from aqueous solution. | a new sight of obtaining a high efficient biosorbent by supplying specific salts into bacterial growth medium was investigated in this study for pb(ii). among a series of salts including na2so4, na2s2o3, kcl, and k2so4, the highest pb(ii) removal efficiency was observed by psychrotrophilic pseudomonas putida i3 grown in the presence of 30g/l k2so4 (ksi3-30) with biosorption capacity of 62.89mg/g under cold condition (15°c), which was increased by 42.35% as compared to control (without any additi ... | 2017 | 28219053 |
| structure of a lov protein in apo-state and implications for construction of lov-based optical tools. | unique features of light-oxygen-voltage (lov) proteins like relatively small size (~12-19 kda), inherent modularity, highly-tunable photocycle and oxygen-independent fluorescence have lately been exploited for the generation of optical tools. structures of lov domains reported so far contain a flavin chromophore per protein molecule. here we report two new findings on the short lov protein w619_1-lov from pseudomonas putida. first, the apo-state crystal structure of w619_1-lov at 2.5 å resolutio ... | 2017 | 28211532 |