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induction of triploid citrus plants from endosperm calli in vitro.triploid hybrid citrus plants were regenerated by somatic embryogenesis in vitro from endosperm derived calli. a sequence of media formulations was used to induce and support proliferation of primary callus from endosperm, to induce embryogenesis from primary callus, and to allow embryo development leading to viable plantlets. calli were induced from cellular endosperm of citrus sinensis (sweet orange), c. xparadisi (grapefruit), and c. grandis (pummelo) excised 12-14 weeks post-anthesis. induct ...199024221109
cryopreservation of nucellar cells of navel orange (citrus sinensis osb. var. brasiliensis tanaka) by vitrification.the nucellar cells of navel orange(citrus sinensis osb. var. brasiliensis tanaka) were successfully cryopreserved by vitrification. in this method, cells were sufficiently dehydrated with highly concentrated cryoprotective solution(pvs2) prior to direct plunge in liquid nitrogen. the pvs2 contains(w/v) 30% glycerol, 15% ethylene glycol and 15% dmso in murashige-tucker medium(mt) containing 0.15 m sucrose. cells were treated with 60% pvs2 at 25°c for 5 min and then chilled pvs2 at 0°c for 3 min. ...199024226373
ethylene-enhanced catabolism of [c]indole-3-acetic acid to indole-3-carboxylic acid in citrus leaf tissues.exogenous [(14)c]indole-3-acetic acid (iaa) is conjugated in citrus (citrus sinensis) leaf tissues to one major substance which has been identified as indole-3-acetylaspartic acid (iaasp). ethylene pretreatment enhanced the catabolism of [(14)c]iaa to indole-3-carboxylic acid (ica), which accumulated as glucose esters (icgiu). increased formation of icgiu by ethylene was accompanied by a concomitant decrease in iaasp formation. iaasp and icgiu were identified by combined gas chromatography-mass ...199016667265
characterization of abscisic acid-induced ethylene production in citrus leaf and tomato fruit tissues.abscisic acid (aba) significantly stimulated ethylene production in citrus (citrus sinensis [l.] osbeck, cv shamouti orange) leaf discs. the extent of stimulation was dependent upon the concentration of aba (0.1-1 milimolar) and the duration of treatment (15-300 minutes). aging the discs before applying aba increased aba-induced ethylene production due to enhancement of both ethylene-forming enzyme activity and the responsiveness of aba. discs excised from mature leaves were much more responsive ...199016667264
plantlet regeneration from a nacl-selected salt-tolerant callus culture of shamouti orange (citrus sinensis l. osbeck).plantlets were regenerated from a selected salt-tolerant cell line of shamouti orange (citrus sinensis l. osbeck). embryogenesis was carried out both in the presence and absence of nacl, yielding green and white globular embryos, respectively. greening could be induced subsequently and normal heart shape embryo development was obtained. plantlet formation required exposure to kinetin prior to the introduction of the root-inducing hormone naphthalene acetic acid. this system differs from the desi ...198924240461
freezing tolerance of citrus, spinach, and petunia leaf tissue : osmotic adjustment and sensitivity to freeze induced cellular dehydration.seasonal variations in freezing tolerance, water content, water and osmotic potential, and levels of soluble sugars of leaves of field-grown valencia orange (citrus sinensis) trees were studied to determine the ability of citrus trees to cold acclimate under natural conditions. controlled environmental studies of young potted citrus trees, spinach (spinacia pleracea), and petunia (petunia hybrids) were carried out to study the water relations during cold acclimation under less variable condition ...198916666563
intergeneric somatic hybrid plants of citrus sinensis cv. hamlin and poncirus trifoliata cv. flying dragon.intergeneric somatic hybrid plants between 'hamlin' sweet orange [citrus sinensis (l.) osbeck] and 'flying dragon' trifoliate orange (poncirus trifoliata raf.) were regenerated following protoplast fusion. 'hamlin' protoplasts, isolated from an habituated embryogenic suspension culture, were fused chemically with 'flying dragon' protoplasts isolated from juvenile leaf tissue. the hybrid selection scheme was based on complementation of the regenerative ability of the 'hamlin' protoplasts with the ...198824241403
role of internal potassium in maintaining growth of cultured citrus cells on increasing nacl and cacl(2) concentrations.shamouti orange (citrus sinensis l. osbeck) salt-tolerant cells were grown under low water potential conditions induced by polyethylene glycol (peg), nacl, and cacl(2). on the basis of equal osmotic potentials, peg was the least inhibitory, nacl next, and cacl(2) the most inhibitory. the relation between growth capacity and ion content can be summarized as follows. (a) internal k(+) concentration was a major factor which changed in the presence of peg, nacl, and cacl(2) and probably played a key ...198716665716
uniformity of plants regenerated from orange (citrus sinensis osb.) protoplasts.using 25 plants (protoclones) regenerated from orange (citrus sinensis osb.) protoplasts, several characters, including leaf and flower morphology, leaf oil, isozyme patterns and chromosome number, were examined. no significant variations in each character were recorded among the protoclones. uniformity observed among protoclones was identical to that of nucellar seedlings.198724241449
levels of free and conjugated abscisic acid in developing floral organs of the navel orange (citrus sinensi [l.] osbeck cv washington).the levels of abscisic acid (aba) and alkaline-hydrolyzable aba-conjugate (putatively identified as the glucosyl ester, abscisyl-beta-d-glucopyranoside) were determined by enzyme immunoassay in the organs of developing navel orange (citrus sinensis [l.] osbeck cv washington) flowers. although both compounds were detected in every tissue, developmentally related differences between organs in the total and relative contents were observed. the highest aba levels were observed in the stigma/style sh ...198616665156
accumulation of chlorophyll, chloroplastic proteins, and thylakoid membranes during reversion of chromoplasts to chloroplasts in citrus sinensis epicarp.in vitro culture of pericarp segments from fruit of citrus sinensis (l.) osbeck cv valencia was used to determine the temporal sequence in development of chloroplasts from chromoplasts during regreening of the epicarp. regreening of chromoplasts closely resembled greening of etioplasts, except that regreening proceeded much more slowly. chlorophyll, the light-harvesting chlorophyll a/b binding protein of photosystem ii, the chlorophyll a binding protein of reaction center p-700 of photosystem i, ...198616664794
resistance of citrus fruit to mass transport of water vapor and other gases.the resistance of oranges (citrus sinensis l. osbeck) and grapefruit (citrus paradisi macf.) to ethylene, o(2), co(2), and h(2)o mass transport was investigated anatomically with scanning electron microscope and physiologically by gas exchange measurements at steady state. the resistance of untreated fruit to water vapor is far less than to ethylene, co(2) and o(2). waxing partially or completely plugs stomatal pores and forms an intermittent cracked layer over the surface of fruit, restricting ...198516664527
somatic hybrid plants obtained by protoplast fusion between citrus sinensis and poncirus trifoliata.somatic hybrid plants of rutaceae were obtained by protoplast fusion between citrus sinensis osb. ('trovita' orange) and poncirus trifoliata. protoplasts isolated from embryogenic cells of c. sinensis and from leaves of p. trifoliata, and the culture of fusion products in the presence of high concentrations of sucrose were essential requirements for the selection of hybrids. green globular embryoids derived from protoplasts resulted in the regeneration of trifoliate plants. other morphological c ...198524247330
photosynthetic activity in the flower buds of ;valencia' orange (citrus sinensis [l.] osbeck).flower buds of ;valencia' orange (citrus sinensis [l.] osbeck) were able to fix (14)co(2) into a number of compounds in their own tissues under both light and dark conditions. the total incorporation, however, was about 4-fold higher in the light than in the dark. in the light, 50% of the total (14)c label was found in the neutral fraction (sugars), 22% in the basic fraction (amino acids), and 26% in the acid-1 fraction (organic acids). in the dark, about 95% of the (14)c label was incorporated ...198516664257
sugar regulation of plastid interconversions in epicarp of citrus fruit.seasonal transformations between chloroplasts and chromoplasts, as measured by changes in chlorophyll content, in the epicarp of degreening and regreening citrus sinensis (l.) osbeck cv valencia fruit closely parallelled the accumulation and later loss of soluble sugars. at any stage of development, reversing the relative soluble sugar content in the epicarp by culturing pericarp segments on agar media with low (15 millimolar) or high (150 millimolar) sucrose concentrations reversed the directio ...198416663837
aspects of salt tolerance in a nacl-selected stable cell line of citrus sinensis.a nacl-tolerant cell line which was selected from ovular callus of ;shamouti' orange (citrus sinensis l. osbeck) proved to be a true cell line variant. this conclusion is based on the following observations. (a) cells which have been removed from the selection pressure for at least four passages retain the same nacl tolerance as do cells which are kept constantly on 0.2 molar nacl. (b) na(+) and cl(-) uptake are considerably lower in salt-tolerant cells (r-10) than in salt-sensitive cells (l-5) ...198316663067
chlorophyllase activity in chlorophyll-free citrus chromoplasts.chromoplast fractions from mature, chlorophyll-less 'valencia' orange (citrus sinensis l. osbeck) flavedo (= the outer coloured layer of citrus peel) showed considerable chlorophyllase activity. acetone powders prepared from chromoplast fractions had 2.5× higher specific activity than those prepared from whole flavedo. exposure of mature, chlorophyll-less fruit to ethylene caused a 2.5 to 4.0 fold increase in chlorophyllase activity. juice chromoplasts showed negligible chlorophyllase activity. ...198324257977
effect of ethylene on [c]indole-3-acetic acid metabolism in leaf tissues of woody plants.the effect of ethylene on [(14)c]indole-3-acetic acid (iaa) metabolism was investigated in defoliation sensitive leaf tissues of citrus (citrus sinensis) and resistant leaf tissues of eucalyptus (eucalyptus camaldulensis). iaa metabolites were fractionated into 80% ethanol-soluble, h(2)o-soluble, naoh-soluble, and insoluble components. in citrus, pretreatment with 25 microliters per liter ethylene for 24 hours significantly increased the amount of ethanol- and h(2)o-extractable conjugates during ...198216662665
stimulation of ethylene production in citrus leaf discs by mannitol.wound ethylene formation induced in leaf tissue of citrus (citrus sinensis [l.] osbeck cv. "washington navel") by excision was significantly stimulated by mannitol after a lag period of about 6 hours. the extent of stimulation was dependent upon the concentration of mannitol (10 to 100 millimolar). this increased ethylene production was not simply due to osmotic effect or water stress as other osmoticums tested failed to exert such an effect. the stimulatory effect of mannitol resulted from both ...198216662435
effects of exogenous ethylene on ethylene production in citrus leaf tissue.exogenous ethylene stimulated ethylene production in intact citrus (citrus sinensis l. osbeck cv. "washington navel") leaves and leaf discs following a 24-hour exposure. studies with leaf discs showed that ethylene production decreased when ethylene was removed by aeration. the extent of stimulation was dependent upon the concentration of exogenous ethylene (1-10 microliters per liter). silver ion blocked the autocatalytic effect of ethylene at concentrations of 0.5 millimolar and lower, but inc ...198216662434
inhibition of ethylene biosynthesis by aminoethoxyvinylglycine and by polyamines shunts label from 3,4-[c]methionine into spermidine in aged orange peel discs.the flux of radioactivity from 3,4-[(14)c]methionine into s-adenosyl-l-methionine (sam), 1-aminocyclopropane-1-carboxylic acid (acc), spermine, and spermidine while inhibiting conversion of acc to ethylene by 100 millimolar phosphate and 2 millimolar co(2+) was studied in aged peel discs of orange (citrus sinensis l. osbeck) fruit. inhibition up to 80% of ethylene production by phosphate and cobalt was accompanied by a 3.3 times increase of label in acc while the radioactivity in sam was only sl ...198216662214
accumulation of free proline in citrus leaves during cold hardening of young trees in controlled temperature regimes.free proline increased in leaves of orange (citrus sinensis [l.] osb. cv. valencia) and grapefruit (citrus paradisi macfad. cv. star ruby) trees on a wide range of citrus rootstocks during cold hardening. increases in sugars accompanied proline accumulation. during cold hardening, the rate of proline accumulation was greater in old than in young leaves. in leaves of grapefruit trees kept in the dark during cold hardening, neither proline nor sugars increased and the degree of cold hardiness was ...197916660980
inhibition of polar indole-3-acetic acid transport by cycloheximide.cycloheximide inhibits polar indoleacetic acid transport in midrib tissues of leaves of citrus (citrus sinensis [l.] osbeck) and poplar (populus deltoides bartr.) as measured by the donor-receiver agar cylinder technique. it appears that the mechanism of auxin transport inhibition by cycloheximide consists in arresting protein synthesis and not in the disruption of energy flow. the interpretation of the data takes into account the involvement of either a carrier protein or auxin-induced proton e ...197916660887
role of ethylene in abscisic acid-induced callus formation in citrus bud cultures.the induction of callus formation in cultured buds of shamouti orange (citrus sinensis [l.] osbeck) by abscisic acid (aba) is a multiphasic process. (altman, and goren 1974 physiol plant 32: 55.) a study of the mediation by ethylene on this effect of aba was undertaken. it was found that: (a) ethylene and (2-chloroethyl) phosphonic acid, as well as aba, induced callus formation; (b) callus induction is best attained when explants are exposed to ethylene during the 1st day after excision; and (c) ...197916660713
abscission of citrus leaf explants: no correlation with naphthaleneacetic acid conjugation in the abscission zone.the role of alpha-naphthaleneacetic acid (naa) in the control of abscission in citrus (citrus sinensis l. osbeck) leaf explants and its conjugation were studied in non-aged and 24-hour-aged explants. dipping non-aged explants in 1.5 micromolar naa for 15 minutes immediately after excision did not delay abscission whereas 150 micromolar naa effectively delayed it. as incubation time was prolonged up to 24 hours after excision, the delaying effect of both concentrations gradually increased. in gen ...197816660505
differential effects of sucrose, abscisic acid, and benzyladenine on shoot growth and callus formation in the abscission zone of excised citrus buds.the omission of sucrose from the basal medium stimulated callus formation in bud explants of citrus sinensis (l.) osbeck. moreover, it increased the abscisic acid-induced callus proliferation reported earlier in the presence of 5% sucrose (altman and goren, physiol. plant. 32: 55, 1974). the inhibition of callus formation by the addition of sucrose was not due to the high osmotic potential of the medium. benzyladenine induced callus formation slightly, in all sucrose concentrations up to 5%. the ...197716660013
differential counteraction of ethylene effects by gibberellin a(3) and n(6)-benzyladenine in senescing citrus peel.treatment of mature citrus fruit (citrus sinensis) with ethylene induced rapid chlorophyll destruction, a rise in respiration, a release of free amino acids, an accumulation of reducing sugars, and an appearance of phenylalanine ammonia lyase activity. gibberellin a(3) (ga(3)) and n(6)-benzyladenine (ba) opposed the effects of ethylene on chlorophyll, amino acids, and to a lesser extent, reducing sugar levels. the ethylene-induced respiratory rise was only slightly modified by ga(3) and ba. phen ...197716659815
endogenous abscisic acid in relation to bud growth in alternate bearing ;valencia' orange.an investigation was conducted into the relation of aba (cis-trans-abscisic acid) in the dormant buds of alternate bearing ;valencia' orange (citrus sinensis [l.] osbeck) trees. aba did not appear to be related to alternate bearing but t-aba (2-transabscisic acid) did. there was 5- to 10-fold more t-aba than aba in the buds. there was more t-aba in the buds of the "on" trees than in the buds of the "off" trees, and a drastic drop in t-aba in both types of buds as spring growth approached. bud do ...197616659743
ethylene-induced formation of aba in citrus peel as related to chloroplast transformations.when mature green harvested shamouti oranges (citrus sinensis l. osbeck) were exposed to 35 mul/liter of ethylene, a 3-fold increase in free abscisic acid (aba) of the flavedo could be detected after 12 hours and a 10-fold increase after 24 hours, while chlorophyll destruction did not exceed 20%. the increase in free aba continued up to 24 hours and leveled off. bound aba accumulated strongly after 24 hours suggesting that excess of free aba was being converted into the bound form. similar incre ...197616659682
involvement of endogenous ethylene in the induction of color change in shamouti oranges.reducing the level of endogenous ethylene in detached shamouti oranges (citrus sinensis l. osbeck) by means of subatmospheric pressure did not alter the rate of chlorophyll destruction and color changes during the first 8 days after harvest in the presence or absence of exogenous ethylene. reducing the activity of ethylene by means of co(2)-known to be a competitive inhibitor for ethylene-inhibited chlorophyll destruction and color change in shamouti oranges ventilated with ethylene, but had no ...197616659580
pectin-polygalacturonase in citrus.extracts from citrus [citrus sinensis (l.) osbeck] pedicles rated as containing polygalacturonase and cellulase gave activities both as measured with carboxymethylcellulose and with sodium polypectate. gel electrophrosis of the extract demonstrated two region areas that contained cellulytic activity.197624430758
partial purification of a growth factor from orange juice which affects citrus tissue culture and its replacement by citric acid.orange (citrus sinensis l. osbeck) tissue cultures required a supply of orange juice to the medium for their vigorous growth. the growth-promoting activity of juice seemed to involve both cell division and cell enlargement. juice had no promotive activity in bioassays for auxins, gibberellins, and cytokinins. the growth promoting activity of juice was mostly transferred into 1-butanol upon partition at ph 2. gas chromatographic analysis of this acid 1-butanol fraction revealed large amounts of c ...197516659287
the effects of ph, ionic strength, and ethylene on the extraction of cellulase from abscission zones of citrus leaf explants.the solubility of cellulase extracted from the abscission zones of citrus leaf explants (citrus sinensis l. osbeck) in sodium phosphate buffer depends on the ph of the extracting solution and, to a lesser extent, on the ionic strength. by increasing molarity from 0.01 to 0.16, the solubility of cellulase increased from 51% to 89% at ph 6.1 and from 70% to 98% at ph 7. in all cases, residual cellulase was further extracted from the pellet by buffer containing 1 m nacl. most of the enzymic activit ...197516659196
metabolism of uronic acids in plant tissues: partial purification and properties of uronic acid oxidase from citrus leaves.a new enzyme, named uronic acid oxidase, was extracted and purified 67-fold by (nh(4))(2)so(4) fractionation and cm-sephadex column chromatography from ethylene-treated shamouti orange (citrus sinensis l. osbeck) leaves. the enzyme catalyzes the oxidation of d-galacturonic acid and d-glucuronic acid to the corresponding hexaric acids in the presence of molecular oxygen with the production of h(2)o(2). the ph optimum for the oxidation of d-galacturonic acid and d-glucuronic acid is between 7 and ...197516659133
effect of water stress on ethylene production by detached leaves of valencia orange (citrus sinensis osbeck).detached leaves of valencia oranges, citrus sinensis osbeck, emanated ethylene at markedly higher rates and contained more endogenous ethylene when placed under water stress at 55% relative humidity than when placed in water-saturated air. water stress induced defoliation from detached branches. relieving the water stress of such leaves by transfer to a mist chamber resulted in lowering of the rates of ethylene emanation to the level occurring in leaves maintained continuously in a mist chamber. ...197416658805
changes in cellulase and pectinase activities in fruit tissues and separation zones of citrus treated with cycloheximide.cellulase activity increased in separation-zone tissues 1 day after "valencia" orange (citrus sinensis [l.] osbeck) was treated with 20 micrograms per milliliter cycloheximide. exocellulase was detected only in the separation zones of treated fruit, whereas endocellulase was present in zones from both treated and control fruit. endocellulase activity in separation-zone tissue of treated fruit was nearly three times as great as that in control tissues. cellulase activity was restricted to separat ...197316658383
adventive plants from ovules and nucelli in citrus.1- to 8-week-old ovules and nucelli from three citrus cultivars-shamouti and valencia (citrus sinensis) oranges and marsh seedless (c. paradisi) grapefruit-were cultured in vitro. no embryo differentiation was observed in the explants prior to culture. the shamouti ovules had degenerated and were apparently unfertilized. embryoids formed on murashige and tucker nutrient medium supplemented with 500 mg/l malt extract. whole plants developed on the same basal medium supplemented with kinetin and i ...197224477995
peel oils of different types of citrus sinensis l. and c. aurantium l. growing in egypt. 19715572114
inhibition of murine subcutaneous and intravenous benzo(rst)pentaphene. carcinogenesis by sweet orange oils and d-limonene. 19715090417
extensibility of pericarp tissue in growing citrus fruits.the tensile force existing in the pericarp of a growing citrus (citrus sinensis) fruit 17 to 19 centimeters in circumference was sufficiently high to cause a 3% shrinkage of the pericarp when it was excised. when a fruit was cut along the equator to the central axis, shrinkage of the pericarp resulted in the formation of a wedge-shaped gap at the cut. stretch modulus of the pericarp was determined by measuring the force required to stretch excised strips of tissue to 1% longer than their excised ...197016657546
water potential components in growing citrus fruits.growing navel orange fruits (citrus sinensis) 5.4 to 5.7 centimeters in diameter were used as a model system to determine the effects of transpiration and carbohydrate translocation on water and osmotic potentials in fruit tissues. evidence supported the hypothesis that osmotic potential in the vesicles would be affected little by changes in transpiration or carbohydrate translocation because the vesicles are anatomically isolated from the transpiration stream and are at the end of the carbohydr ...197016657407
site of fluoride accumulation in navel orange leaves.fluoride-polluted navel orange leaves, citrus sinensis (linn.) osbeck, were fractionated into the subcellular components in hexane/carbon tetrachloride mixtures having various densities. fluoride was determined at each fraction. analyses were also made for the subcellular distribution of chlorophyll, nitrogen, and dna to assess the extent of cross-contamination of each component. the fraction containing cell wall, nuclei, and partly broken cells apparently contained a major amount of fluoride. h ...19665908632
cyanide & chelating agent effects on in vitro co(2) fixation in sweet orange leaves. 196116655460
purification and identification of flavanone glycosides in the peel of the sweet orange. 196013818662
oil of sweet orange: a possible role in carcinogenesis. 195913662498
[first supplement to the bibliography on citrus sinensis]. 200913125380
[recurrent dermatitis of the hands caused by contact with oranges; intolerance to sweet orange juice]. 195313118905
constitution of the juice lipids of the florida valencia orange (citrus sinensis l.). 201614840502
relationships between xylem anatomy, root hydraulic conductivity, leaf/root ratio and transpiration in citrus trees on different rootstocks.the aim of the study was to determine the extent in which leaf and whole plant transpiration (tp) were influenced by root hydraulic conductance (k(r)), leaf to root ratio and leaf mass. also, the relationships between the anatomic characteristics of roots and k(r) were investigated. to this end, 9-month-old seedlings of the citrus rootstocks cleopatra mandarin (cm), poncirus trifoliata (pt), and their hybrids forner-alcaide no 5 (fa-5) and forner-alcaide no 13 (fa-13) and 15-month-old trees of v ...201020088906
regulation of ascorbic acid metabolism by blue led light irradiation in citrus juice sacs.in the present study, the effects of red and blue led lights on the accumulation of ascorbic acid (asa) were investigated in the juice sacs of three citrus varieties, satsuma mandarin, valencia orange, and lisbon lemon. the results showed that the blue led light treatment effectively increased the asa content in the juice sacs of the three citrus varieties, whereas the red led light treatment did not. by increasing the blue led light intensity, the juice sacs of the three citrus varieties accumu ...201525711821
sclerenchymatous ring as a barrier to phloem feeding by asian citrus psyllid: evidence from electrical penetration graph and visualization of stylet pathways.asian citrus psyllid (diaphorina citri) feeding behaviors play a significant role in the transmission of the phloem-limited candidatus liberibacter asiaticus (clas) bacterium that causes the economically devastating citrus greening disease. sustained phloem ingestion by d. citri on clas infected plants is required for pathogen acquisition and transmission. recent studies have shown a fibrous ring of thick-walled sclerenchyma around the phloem in mature, fully expanded citrus leaves that is more ...201728278248
stylet morphometrics and citrus leaf vein structure in relation to feeding behavior of the asian citrus psyllid diaphorina citri, vector of citrus huanglongbing bacterium.the asian citrus psyllid (acp), diaphorina citri (hemiptera: psyllidae), is the primary vector of the phloem-limited bacterium candidatus liberibacter asiaticus (las) associated with huanglongbing (hlb, citrus greening), considered the world's most serious disease of citrus. stylet morphometrics of acp nymphs and adults were studied in relation to citrus vein structure and to their putative (histologically verified) feeding sites on valencia orange leaves. acp nymphs preferred to settle and feed ...201323555830
effect of physiological and experiential state ofbactrocera tryoni flies on intra-tree foraging behavior for food (bacteria) and host fruit.using caged host trees on which we manipulated food and oviposition sites, we investigated the foraging behavior of individually-releasedbactrocera tryoni (diptera: tephritidae) females in relation to state of fly hunger for protein, presence or absence of bacteria as a source of protein, degree of prior experience with host fruit, and quality of host fruit for oviposition. one aim was to evaluate whether it is immature or matureb. tryoni females that are responsible for initially inoculating ho ...199128313268
correlation between classification in risk categories and clinical aspects and outcomes.to correlate classification in risk categories with the clinical profiles, outcomes and origins of patients.201627982310
effect of the use of a neutralizing step after antimicrobial application on microbial counts during challenge studies for orange disinfection.the effects of using a neutralizer after applying antimicrobial treatments and the effect of time lapse between treatment application and subsequent recovery and enumeration of escherichia coli o157:h7 and salmonella were investigated in valencia oranges. inoculated oranges surfaces were washed with distilled water for 15 s and then sprayed with a solution containing 200 mg/liter sodium hypochlorite (ph 6.5) for 15 s; they were then dipped in l-lactic acid (2.0% at 55°c) for 1 min or in distille ...201323433383
draft genome sequence of fructophilic lactobacillus florum.herein we report the first genome sequence for lactobacillus florum. l. florum 2f was isolated from valencia orange leaves and is fructophilic, like other strains of this species. the draft genome of l. florum 2f contains 1,261,842 bp with a g+c content of 41.5% in 46 contigs (≥500 bp).201323405360
isolation and identification of fungi associated with spoilt fruits vended in gwagwalada market, abuja, nigeria.annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. this study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in gwagwalada market, abuja, and recommend appropriate control measure.201728507410
anti-quorum sensing activity of some medicinal plants.quorum sensing is the key regulator of virulence factors of pseudomonas aeruginosa such as biofilm formation, motility, productions of proteases, hemolysin, pyocyanin, and toxins. the aim of this study was to explore the effect of the extracts from some medicinal plants on quorum sensing and related virulence factors of p. aeruginosa.201628487896
identification and characterization of citrus tristeza virus isolates breaking resistance in trifoliate orange in california.most citrus tristeza virus (ctv) isolates in california are biologically mild and symptomless in commercial cultivars on ctv tolerant rootstocks. however, to better define california ctv isolates showing divergent serological and genetic profiles, selected isolates were subjected to deep sequencing of small rnas. full-length sequences were assembled, annotated and trifoliate orange resistance-breaking (rb) isolates of ctv were identified. phylogenetic relationships based on their full genomes pl ...201728453412
compositional shift in fatty acid profiles of lipids obtained from oleaginous yeasts upon the addition of essential oil from citrus sinensis l.tailoring lipids from oleaginous yeasts to contain specific types of fatty acid is of considerable interest to food, fuel, and pharmaceutical industries. in this study, the essential oil obtained from citrus sinesus l. has been used to alter the fatty acid composition of two common oleaginous yeasts, rhodosporidium toruloides and cryptococcus curvatus. with increasing levels of essential oil in the medium, the metabolic flux of the fatty acid biosynthesis pathway shifted towards saturated fatty ...201728474217
effects of heat treatment on the antioxidative and anti-inflammatory properties of orange by-products.this study investigated the changes in the functional components, antioxidative activities, antibacterial activities, anti-inflammatory activities of orange (citrus sinensis (l.) osbeck) by-products (obp) on heat treatment at 50 and 100 °c (hereafter denoted 50d and 100d extracts, respectively). optimal heating conditions were also investigated. the total phenolic content, flavonoid content and antioxidative activities of obp extracts significantly increased on heat treatment. the lag time of cu ...201728653733
cytotoxicity and anti-leishmania amazonensis activity of citrus sinensis leaf extracts.leishmania amazonensis is the main agent of diffuse cutaneous leishmaniasis, a disease characterized by lesional polymorphism and the commitment of skin surface. previous reports demonstrated that the citrus genus possess antimicrobial activity.201728524774
first report of phyllosticta citricarpa and description of two new species, p. paracapitalensis and p. paracitricarpa, from citrus in europe.the genus phyllosticta occurs worldwide, and contains numerous plant pathogenic, endophytic and saprobic species. phyllosticta citricarpa is the causal agent of citrus black spot disease (cbs), affecting fruits and leaves of several citrus hosts (rutaceae), and can also be isolated from asymptomatic citrus tissues. citrus black spot occurs in citrus-growing regions with warm summer rainfall climates, but is absent in countries of the european union (eu). phyllosticta capitalensis is morphologica ...201728720979
quantification of transfer of salmonella from citrus fruits to peel, edible portion, and gloved hands during hand peeling.although studies have quantified bacterial transfer between hands and various materials, cross-contamination between the surface of fresh citrus fruit and the edible portions during hand peeling has not been reported. this study quantifies transfer of salmonella to the edible portion of citrus fruit from a contaminated peel during hand peeling. citrus fruits used for this study were citrus sinensis (sweet orange) cultivars 'valencia' and 'navel', citrus unshiu (satsuma mandarins), citrus reticul ...201728463082
crystal structure and regulation of the citrus pol iii repressor maf1 by auxin and phosphorylation.maf1 is the main rna polymerase (pol) iii repressor that controls cell growth in eukaryotes. the citrus ortholog, csmaf1, was shown to restrict cell growth in citrus canker disease but its role in plant development and disease is still unclear. we solved the crystal structure of the globular core of csmaf1, which reveals additional structural elements compared with the previously available structure of hmaf1, and explored the dynamics of its flexible regions not present in the structure. csmaf1 ...201728781084
ectopic expression of xylella fastidiosa rpff conferring production of diffusible signal factor in transgenic tobacco and citrus alters pathogen behavior and reduces disease severity.the pathogenicity of xylella fastidiosa is associated with its ability to colonize the xylem of host plants. expression of genes contributing to xylem colonization are suppressed, while those necessary for insect vector acquisition are increased, with increasing concentrations of diffusible signal factor (dsf) whose production is dependent on rpff. we previously demonstrated that transgenic citrus plants ectopically expressing rpff from a citrus strain of x. fastidiosa subsp. pauca exhibited les ...201728777044
proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of xanthomonas citri subsp. citri during citrus sinensis infection.xanthomonas citri subsp. citri (xac) is the causal agent of citrus canker. a proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of xac during infection.201728693412
sulfate-binding protein (sbp) from xanthomonas citri: structure and functional insights.the uptake and transport of sulfate in bacteria is mediated by an atp-binding cassette transporter (abc transporter) encoded by sbpcysuwa genes, whose importance has been widely demonstrated due to their relevance in cysteine synthesis and bacterial growth. in xanthomonas citri, the causative agent of canker disease, the expression of components from this abc transporter and others related to uptake of organic sulfur sources has been shown during in vitro growth cultures. in this work, based on ...201728562158
carotenoid profiling of red navel orange "cara cara" harvested from five regions in china.understanding the composition and content of carotenoids in red-flesh navel orange "cara cara" cultivated in different regions is valuable for its nutrition assessment and fruit processing control. herein, the carotenoids of "cara cara" fruits, harvested from five typical citrus growing regions of china, were identified and quantified by hplc-dad coupled with hplc-dad-apci-ms/ms. in saponified extracts, 19 free carotenoids including 8 carotenes and 11 xanthophylls were identified. in unsaponifie ...028490141
comparative study on ambient ionization methods for direct analysis of navel orange tissues by mass spectrometry.it is of sustainable interest to improve the sensitivity and selectivity of the ionization process, especially for direct analysis of complex samples without matrix separation. herein, four ambient ionization methods including desorption atmospheric pressure chemical ionization (dapci), heat-assisted desorption atmospheric pressure chemical ionization (heat-assisted dapci), microwave plasma torch (mpt), and internal extractive electrospray ionization (ieesi) were employed for comparative analysi ...028628730
influence of postharvest treatments on qualitative and chemical parameters of tarocco blood orange fruits to be used for fresh chilled juice.tarocco "sant'alfio" is a late ripening blood orange cultivar. blood oranges are more and more appreciated from consumers for their high nutraceutical value due to the presence of bioactive compounds including vitamin c, polyphenols, flavonoids and hydroxycinnamic acids. the aim of this work is to set up a reliable protocol for postharvest storage of the very-late tarocco "sant'alfio" orange to prolong the availability of this product in the market to be used for fresh chilled orange juice produ ...028407933
qtl mapping for fruit quality in citrus using dartseq markers.citrus breeding programs have many limitations associated with the species biology and physiology, requiring the incorporation of new biotechnological tools to provide new breeding possibilities. diversity arrays technology (dart) markers, combined with next-generation sequencing, have wide applicability in the construction of high-resolution genetic maps and in quantitative trait locus (qtl) mapping. this study aimed to construct an integrated genetic map using full-sib progeny derived from mur ...028403819
differentiation using microwave plasma torch desorption mass spectrometry of navel oranges cultivated in neighboring habitats.the molecular fingerprinting of intact fruit samples combined with statistical data analysis can allow the assessment of fruit quality and location of origin. herein, microwave plasma torch desorption ionization mass spectrometry (mpt-ms) was applied to produce molecular fingerprints for the juice sac and exocarp of navel oranges cultivated in three closely located habitats, and the mass spectrometric fingerprints were differentiated by principal component analysis (pca). because of the relative ...028269986
influence of orange cultivar and mandarin postharvest storage on polyphenols, ascorbic acid and antioxidant activity during gastrointestinal digestion.polyphenols, ascorbic acid content and antioxidant activity of two sweet oranges (navel-n and cara cara-cc) and mandarin (clementine-m) as well as their bioaccessibilities were evaluated in pulps and compared to those in fresh juice. thus, pulps of oranges and mandarins displayed higher hesperidin (hes), narirutin (nar), total flavonoids (tf), total phenols (tp) and antioxidant activity (aac) than their corresponding juices. also, cc products presented higher bioactive compounds content than n o ...028193404
led blue light-induced changes in phenolics and ethylene in citrus fruit: implication in elicited resistance against penicillium digitatum infection.the objective was to investigate whether led blue light (lbl) induces changes in phenolics and ethylene production of sweet oranges, and whether they participate in lbl-elicited resistance against the most important postharvest pathogen (penicillium digitatum) of citrus fruit. the expression of relevant genes of the phenylpropanoid and ethylene biosynthetic pathways during elicitation of resistance was also determined. different lbl (wavelength 450nm) quantum fluxes were used within the 60-630μm ...027719952
[influence of spectral pre-processing on pls quantitative model of detecting cu in navel orange by libs].cu in navel orange was detected rapidly by laser-induced breakdown spectroscopy (libs) combined with partial least squares (pls) for quantitative analysis, then the effect on the detection accuracy of the model with different spectral data ptetreatment methods was explored. spectral data for the 52 gannan navel orange samples were pretreated by different data smoothing, mean centralized and standard normal variable transform. then 319~338 nm wavelength section containing characteristic spectral ...026415466
metabolite signature of candidatus liberibacter asiaticus infection in two citrus varieties.huanglongbing (hlb), also known as citrus greening disease, is caused by the bacterium 'candidatus liberibacter asiaticus' (clas) and is a serious threat to the citrus industry. to understand the effect of clas infection on the citrus metabolome, juice from healthy (n = 18), hlb-asymptomatic (n = 18), and hlb-symptomatic hamlin (n = 18), as well as from healthy (n = 18) and hlb-symptomatic (n = 18) valencia sweet oranges (from southern and eastern florida) were evaluated using (1)h nmr-based met ...201424959841
electronic nose application for determination of penicillium digitatum in valencia oranges.penicillium digitatum and penicillium italicum are responsible for one the most serious diseases occurring during storage of citrus fruits. its early detection allows a relevant increase in shelf life, and in situ monitoring of fungal infections represents a very efficient tool to improve storage quality. in the case of metabolic alterations due to physiological or fungal pathologies, olfactometric analysis allows the detection of specific volatile biomarkers, thus providing an effective tool fo ...201222261834
mutations of β-tubulin codon 198 or 200 indicate thiabendazole resistance among isolates of penicillium digitatum collected from citrus in taiwan.penicillium digitatum causes green mold on citrus, resulting in severe postharvest fruit decay and economic losses in many citrus-producing areas of the world. forty isolates of p. digitatum were cultured from citrus groves, packinghouses, and local markets in taiwan, and assessed quantitatively for their sensitivity to thiabendazole (tbz) fungicide. sensitivity assays using a 96-well microtiter plate revealed that, of 40 isolates examined, only one isolate collected from fruit produced in taiwa ...201121868117
the effect of viroid infection of citrus trees on the amoebicidal activity of 'maltese half-blood' (citrus sinensis) against trophozoite stage of acanthamoeba castellanii neff.in order to promote a local tunisian product, this study was designed to examine, for the first time, the anti-acanthamoeba activity (acanthamoeba castellanii neff) of the essential oils of tunisian citrus sinensis peels (maltese half-blood) and the effect of viroid plant infection on this activity. to do so, three samples of peels' essential oils were studied: from a healthy plant (control), a plant inoculated with citrus exocortis viroid (cevd) and one inoculated with hot stunt cachexia viroid ...201728916460
anticancer potential of citrus juices and their extracts: a systematic review of both preclinical and clinical studies.background: during the last decades, a huge body of evidence has been accumulated suggesting that citrus fruits and their juices might have a role in preventing many diseases including cancer. objective: to summarize the numerous evidences on the potential of citrus juices and their extracts as anticancer agents. data sources: a systematic review of articles written in english using medline (1946-present), embase (1974-present) and web of sciences (1970-present) was performed independently by tw ...201728713272
comparative transcriptome analysis of two citrus germplasms with contrasting susceptibility to phytophthora nicotianae provides new insights into tolerance mechanisms.host perception of phytophthora nicotianae switching to necrotrophy is fundamental for disease tolerance of citrus. it involves an hr-like response, strengthening of the cell wall structure and hormonal signaling. stem rot caused by p. nicotianae is a worldwide disease of several important crops, including citrus. given the growing awareness of chemical fungicides drawbacks, genetic improvement of citrus rootstocks remains the best alternative. however, the molecular basis underlying the success ...201729290008
citrus essential oils inhalation by mice: behavioral testing, gcms plasma analysis, corticosterone, and melatonin levels evaluation.the use of orange essential oils (eos) as a complementary treatment is very common in brazilian popular culture. the levels of melatonin (mel) and corticosterone (cort) hormones were investigated simultaneously, by the luminex™ immunoassay system in mice plasma, after citrus aurantium and citrus sinensis eos inhalation for 30 min. the plasma was analyzed by headspace through gas chromatography coupled to mass spectrometry for investigation of the eo components. mice were submitted to behavioral ...201829168240
[study on hplc fingerprint and chemical constituent difference of different species of aurantii fructus immaturus].this study is to establish an hplc fingerprint by hplc-dad method and simultaneous quantitative analysis of 17 components of 18 batches of citrus aurantium and 10 batches of c. sinensis. the separation was performed on an agilent poroshell 120 sb-c₁₈ (4.6 mm×100 mm,2.7 μm) column with the gradient elution of methanol-0.1% formic acid water, the flow was 0.6 ml•min⁻¹. the detection wavelength was set at 318 nm. the column temperature was maintained at 30 ℃. the data calculation was performed with ...201628920382
comparative analysis of juice volatiles in selected mandarins, mandarin relatives and other citrus genotypes.citrus fruit flavor is an important attribute prioritized in variety improvement. the present study compared juice volatiles compositions from 13 selected citrus genotypes, including six mandarins (citrus reticulata), three sour oranges (citrus aurantium), one blood orange (citrus sinensis), one lime (citrus limonia), one clementine (citrus clementina) and one satsuma (citrus unshiu).201828731231
metabolic profile and underlying improved bio-activity of fructus aurantii immaturus by human intestinal bacteria.fructus aurantii immaturus (fai) is the dried young fruit of citrus aurantium l. or citrus sinensis l. osbeck. the purpose of this paper was to investigate the metabolic fate of fai upon incubation with human intestinal bacteria, meanwhile to evaluate the antioxidant and anti-inflammatory activities of fai and the transformed fructus aurantii immaturus (tfai). the water extract of fai was anaerobically incubated with human intestinal bacterial suspensions for 48 h at 37 °c. liquid chromatography ...201728504280
nuclear species-diagnostic snp markers mined from 454 amplicon sequencing reveal admixture genomic structure of modern citrus varieties.most cultivated citrus species originated from interspecific hybridisation between four ancestral taxa (c. reticulata, c. maxima, c. medica, and c. micrantha) with limited further interspecific recombination due to vegetative propagation. this evolution resulted in admixture genomes with frequent interspecific heterozygosity. moreover, a major part of the phenotypic diversity of edible citrus results from the initial differentiation between these taxa. deciphering the phylogenomic structure of c ...201525973611
draft sequencing of the heterozygous diploid genome of satsuma (citrus unshiu marc.) using a hybrid assembly approach.satsuma (citrus unshiu marc.) is one of the most abundantly produced mandarin varieties of citrus, known for its seedless fruit production and as a breeding parent of citrus. de novo assembly of the heterozygous diploid genome of satsuma ("miyagawa wase") was conducted by a hybrid assembly approach using short-read sequences, three mate-pair libraries, and a long-read sequence of pacbio by the platanus assembler. the assembled sequence, with a total size of 359.7 mb at the n50 length of 386,404 ...201729259619
tetraploid carrizo citrange rootstock (citrus sinensis osb.×poncirus trifoliata l. raf.) enhances natural chilling stress tolerance of common clementine (citrus clementina hort. ex tan).low temperatures can disturb the development, growth and geographic distribution of plants, particularly cold-sensitive plants in the mediterranean area, where temperatures can reach seasonally low levels. in citrus crops, scion/rootstock combinations are used to improve fruit production and quality, and increase tolerance to biotic and abiotic stresses. in the last decade, several studies have shown that tetraploid citrus seedlings or rootstocks are more tolerant to abiotic stress than their re ...201728478318
alternative oxidase (aox) constitutes a small family of proteins in citrus clementina and citrus sinensis l. osb.the alternative oxidase (aox) protein is present in plants, fungi, protozoa and some invertebrates. it is involved in the mitochondrial respiratory chain, providing an alternative route for the transport of electrons, leading to the reduction of oxygen to form water. the present study aimed to characterize the family of aox genes in mandarin (citrus clementina) and sweet orange (citrus sinensis) at nucleotide and protein levels, including promoter analysis, phylogenetic analysis and c. sinensis ...201728459876
analysis of bioactive compounds in seven citrus cultivars.chemical content in peels of fruits belonging to cultivars of clementine mandarin (fino, loretina and marisol), satsume mandarin (owari), navel orange (navelate and navelina) and common orange (valencia late) groups have been analyzed. the influence of the variety on the content of bioactive constituents at the varietal level is discussed. rind contents showed similar tendencies for the majority of compounds although satsume group presented the highest amounts on the flavanone glycosides hesperi ...201121364046
pulsed electric fields versus thermal treatment: equivalent processes to obtain equally acceptable citrus juices.pulsed electric field treatment has been claimed to produce more acceptable chilled citrus juices than those obtained by conventional thermal treatment. the pectin methylesterase activity and the acceptability of nine juices obtained from clementine mandarins, valencia oranges, and ortanique fruits (hybrid of mandarin and orange), untreated, pasteurized (85 degrees c for 10 s), and treated by pulsed electric fields (25 kv/cm for 330 micros), were evaluated. the treatments, selected to reach a si ...200616924935
impact of phyllocnistis citrella (lepidoptera: gracillariidae) on leaf area development and yield of mature citrus trees in the mediterranean area.seasonal population trends and damage to citrus trees by the leafminer phyllocnistis citrella stainton were studied in the valencia area of eastern spain from 1996 to 1999. the area-wide seasonal flushing pattern of citrus trees and leafminer population trends were determined in 10 mature citrus orchards. in the 10 orchards, the annual percentage of new shoots that developed in the spring ranged from 51 to 96% for individual orchards (mean of 80%). phyllocnistis citrella populations damaged new ...200212403423
glc analysis of thiabendazole residues in citrus fruit.analysis of thiabendazole (tbz) residues in citrus fruit is performed using a bonded phase, se-54, fused-silica capillary column. the fungicide is extracted with hexane: ethyl acetate (90:10, v/v) at high ph and, after a short cleanup, determined by gas-liquid chromatography with a nitrogen-phosphorus detector (npd). recoveries through the method are always higher than 80% and the limit of detection is 0.01 mg/kg. tbz residues are determined in whole fruit, peel, and pulp of "washington navel" o ...19873558699
gas chromatographic determination of fenpropimorph residues in citrus fruit.a rapid gas chromatographic method for determining fenpropimorph residues in citrus fruit is reported. the fungicide is extracted with hexane after ph adjustment of the fruit homogenate. a short liquid-liquid partitioning process is performed before gas chromatography on an ov-17 column with nitrogen-phosphorus specific detection. the limit of detection of the method was 0.01 mg/kg, based on a 25 g sample. recovery was always higher than 70%. fenpropimorph residues in "washington navel" oranges ...20163771458
residues analysis of post-harvest imidazole fungicides in citrus fruit by hplc and glc.the determination of imazalil and prochloraz fungicide residues has been carried out by hplc with an uv detector at 204 nm and by glc with an electron capture detector (ecd). in both cases fungicide residues were extracted with hexane/acetone (90:10, v/v) after ph adjustment and purified by a liquid-liquid partitioning process. when hplc was used for prochloraz and imazalil analysis, it was necessary to eliminate the interfering substances with a further clean-up process. this was also required ...19854077370
reprogramming of a defense signaling pathway in rough lemon and sweet orange is a critical element of the early response to 'candidatus liberibacter asiaticus'.huanglongbing (hlb) in citrus infected by candidatus liberibacter asiaticus (clas) has caused tremendous losses to the citrus industry. no resistant genotypes have been identified in citrus species or close relatives. among citrus varieties, rough lemon (citrus jambhiri) has been considered tolerant due to its ability to produce a healthy flush of new growth after infection. the difference between tolerance and susceptibility is often defined by the speed and intensity of a plant's response to a ...201729214028
a comparison of anion concentration in surficial groundwater sampled from two types of water quality monitoring wells.groundwater sampling for monitoring the presence and concentration of contaminants can be done using either depth integrated monitoring wells (mw) or depth specific multi-level sampling (mls) wells. depth specific multi-level sampling wells (mls) are cost-effective, easy to install, and provide very detailed information about the vertical gradient in contaminant concentration. in contrast the mw sampling provides information on the presence of contaminant over large representative area. this stu ...200717129947
antimutagenic and antioxidant activity of the essential oils of citrus sinensis and citrus latifolia.the essential oils of citrus sinensis and citrus latifolia showed antimycotic activity against candida spp. isolated from the oral cavity; they are neither mutagenic on the ames test nor cytotoxic. their main components are r-(+)-limonene, β-thujene, α-myrcene and γ-terpinene. the aim of this work was to evaluate their antimutagenic and antioxidant capacities. antimutagenic properties were evaluated against mnng and enng on s. typhimurium ta100; against 2aa on strain ta98 and in front of 4nqo an ...201728904369
two of a kind but different: luminescent carbon quantum dots from citrus peels for iron and tartrazine sensing and cell imaging.citrus sinensis and citrus limon peels were used to synthesize two different kinds of carbon quantum dots (cqds) via an unsophisticated and inexpensive carbonization procedure. the proposed synthesis is straightforward and adheres to the principles of green chemistry since no organic solvents are used and no toxic by-products are formed, while the residual resources employed facilitate the large scale synthesis of dots. the citrus sinensis and citrus limon peels are proved to be excellent precur ...201728841995
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