| purification, cloning, and expression of an alpha/beta-galactoside alpha-2,3-sialyltransferase from a luminous marine bacterium, photobacterium phosphoreum. | a novel sialyltransferase, alpha/beta-galactoside alpha-2,3-sialyltransferase, was purified from the cell lysate of a luminous marine bacterium, photobacterium phosphoreum jt-ish-467, isolated from the japanese common squid (todarodes pacificus). the gene encoding the enzyme was cloned from the genomic library of the bacterium using probes derived from the nh(2)-terminal and internal amino acid sequences. an open reading frame of 409 amino acids was identified, and the sequence had 32% identity ... | 2007 | 17702755 |
| a beta-galactoside alpha2,6-sialyltransferase produced by a marine bacterium, photobacterium leiognathi jt-shiz-145, is active at ph 8. | a gene encoding a sialyltransferase produced by photobacterium leiognathi jt-shiz-145 was cloned, sequenced, and expressed in escherichia coli. the sialyltransferase gene contained an open reading frame of 1494 base pairs (bp) encoding a predicted protein of 497 amino acid residues. the deduced amino acid sequence of the sialyltransferase had no significant similarity to mammalian sialyltransferases and did not contain sialyl motifs, but did show high homology to another marine bacterial sialylt ... | 2007 | 17704107 |
| inferring the evolutionary history of vibrios by means of multilocus sequence analysis. | we performed the first broad study aiming at the reconstruction of the evolutionary history of vibrios by means of multilocus sequence analysis of nine genes. overall, 14 distinct clades were recognized using the splitstree decomposition method. some of these clades may correspond to families, e.g., the clades salinivibrio and photobacteria, while other clades, e.g., splendidus and harveyi, correspond to genera. the common ancestor of all vibrios was estimated to have been present 600 million ye ... | 2007 | 17704223 |
| an algal toxicity database of organic toxicants derived by a closed-system technique. | the current study presents the toxicity data of 90 organic compounds with various modes of actions to pseudokirchneriella subcapitata. the assessment was conducted using a closed-system technique, and a biomass-type end point based on the cell density was employed. the above toxicity data were compared with test results from ciliate (tetrahymena pyriformis), water flea (daphnia magna), fish (pimephales promelas), and luminescent bacteria (photobacterium phosphoreum). satisfactory correlation rel ... | 2007 | 17705651 |
| bioluminescent monitoring of detoxification processes: activity of humic substances in quinone solutions. | this study deals with application of bioluminescent assay systems to evaluate the detoxifying effect of humic substances (hs) on the solutions of organic oxidizers - quinones. a series of homologous quinones with different redox characteristics: 1,4-benzoquinone, tetrafluoro-1,4-benzoquinone, methyl-1,4-benzoquinone, tetramethyl-1,4-benzoquinone, and 1,4-naphtoquinone, was used. bioluminescent bacteria photobacterium phosphoreum, and nadh:fmn-oxidoreductase-luciferase enzyme system isolated from ... | 2007 | 17716903 |
| antimicrobial properties of moderately halotolerant bacteria from cenotes of the yucatan peninsula. | isolation and antimicrobial evaluation of aquatic bacterial strains from two cenotes. | 2007 | 17718841 |
| luciferase from vibrio campbellii is more thermostable and binds reduced fmn better than its homologues. | a new luciferase from v. campbellii (lux_vc) was cloned and expressed in escherichia coli and purified to homogeneity. although the amino acid sequences and the catalytic reactions of lux_vc are highly similar to those of the luciferase from v. harveyi (lux_vh), the two enzymes have different affinities toward reduced fmn (fmnh(-)). the catalytic reactions of lux_vc and lux vh were monitored by stopped-flow absorbance and luminescence spectroscopy at 4 degrees c and ph 8. the measured kd at 4 de ... | 2007 | 17761697 |
| photobacterium kishitanii sp. nov., a luminous marine bacterium symbiotic with deep-sea fishes. | six representatives of a luminous bacterium commonly found in association with deep, cold-dwelling marine fishes were isolated from the light organs and skin of different fish species. these bacteria were gram-negative, catalase-positive, and weakly oxidase-positive or oxidase-negative. morphologically, cells of these strains were coccoid or coccoid-rods, occurring singly or in pairs, and motile by means of polar flagellation. after growth on seawater-based agar medium at 22 degrees c for 18 h, ... | 2007 | 17766874 |
| the genome of epsilon15, a serotype-converting, group e1 salmonella enterica-specific bacteriophage. | the genome sequence of the salmonella enterica serovar anatum-specific, serotype-converting bacteriophage epsilon15 has been completed. the nonredundant genome contains 39,671 bp and 51 putative genes. it most closely resembles the genome of phiv10, an escherichia coli o157:h7-specific temperate phage, with which it shares 36 related genes. more distant relatives include the burkholderia cepacia-specific phage, bcepc6b (8 similar genes), the bordetella bronchiseptica-specific phage, bpp-1 (8 sim ... | 2007 | 17825342 |
| bacterial origin of luminescence in marine animals. | bacterial luciferase activity was detected in light organ extracts of squids, fishes, and pyrosomes, suggesting that these systems are derived from bacteria-animal symbioses. in none of these cases was it possible to culture luminouis bacteria. analyses of the decay kinetics show that the luciferases from the squid, ceratioid, and pyrosome light organs are all similar to bacterial luciferases from the genus photobacterium, while those from the anomalopid light organs are different. | 1980 | 17830813 |
| two distinct photobacterium populations thrive in ancient mediterranean sapropels. | eastern mediterranean sediments are characterized by the periodic occurrence of conspicuous, organic matter-rich sapropel layers. phylogenetic analysis of a large culture collection isolated from these sediments revealed that about one third of the isolates belonged to the genus photobacterium. in the present study, 22 of these strains were examined with respect to their phylogenetic and metabolic diversity. the strains belonged to two distinct photobacterium populations (mediterranean cluster i ... | 2008 | 17874305 |
| toxicity of urban highway runoff with respect to storm duration. | the toxicity of stormwater runoff during various time-based stages was measured in both grab and composite samples collected from three highly urbanized highway sites in los angeles, california between 2002 and 2005. stormwater runoff samples were tested for toxicity using three freshwater species (the water flea ceriodaphnia dubia, the fathead minnow pimephales promelas, and the green algae pseudokirchneriella subcapitatum) and two marine species (the purple sea urchin strongylocentrotus purpur ... | 2008 | 17920106 |
| conservation of the chitin utilization pathway in the vibrionaceae. | vibrionaceae are regarded as important marine chitin degraders, and attachment to chitin regulates important biological functions; yet, the degree of chitin pathway conservation in vibrionaceae is unknown. here, a core chitin degradation pathway is proposed based on comparison of 19 vibrio and photobacterium genomes with a detailed metabolic map assembled for v. cholerae from published biochemical, genomic, and transcriptomic results. further, to assess whether chitin degradation is a conserved ... | 2008 | 17933912 |
| the combined bacterial lux-fluoro test for the detection and quantification of genotoxic and cytotoxic agents in surface water: results from the "technical workshop on genotoxicity biosensing". | the bioassay lux-fluoro test was developed for the rapid detection and quantification of environmental pollutants with genotoxic and/or cytotoxic potential. this bacterial test system uses two different reporter genes whose gene products and their reactions, respectively, can be measured easily and simultaneously by optical methods. genotoxicity is measured by the increase of bioluminescence in genetically modified bacteria which carry a plasmid with a complete lux operon for the enzyme lucifera ... | 2007 | 17936920 |
| cloning and characterization of photobacterium damselae ssp. piscicida phospholipase: an enzyme that shows haemolytic activity. | a phospholipase gene of photobacterium damselae ssp. piscicida (ppp) was cloned from a genomic library and its nucleotide sequence was determined. the open reading frame consisted of 1218 bp encoding a protein of 405 amino acids with a predicted molecular mass of 46 kda. the ppp had identities (53-55%) with phospholipase and haemolysin of vibrio spp., while it showed low identities (23-26%) with glycerophospholipid cholesterol acyltransferase of aeromonas spp. a recombinant ppp (rppp) with a his ... | 2007 | 17958612 |
| crystal structure of vibrionaceae photobacterium sp. jt-ish-224 alpha2,6-sialyltransferase in a ternary complex with donor product cmp and acceptor substrate lactose: catalytic mechanism and substrate recognition. | sialyltransferases are a family of glycosyltransferases that catalyze the transfer of n-acetylneuraminic acid residues from cytidine monophosphate n-acetylneuraminic acid (cmp-neuac) as a donor substrate to the carbohydrate groups of glycoproteins and glycolipids as acceptor substrates. we determined the crystal structure of delta16psp26st, the n-terminal truncated form of alpha2,6-sialyltransferase from vibrionaceae photobacterium sp. jt-ish-224, complexed with a donor product cmp and an accept ... | 2008 | 17962295 |
| gene expression and distribution of antibacterial l-amino acid oxidase in the rockfish sebastes schlegeli. | antibacterial factors in the epidermal mucus of fish have a potential importance in the first line of the host defense response to bacterial pathogens. we previously isolated a novel antibacterial protein termed ssap (sebastes schlegeli antibacterial protein) from the skin mucus of the rockfish s. schlegeli and identified it as a new member of the l-amino acid oxidase (lao) family. in the present study, the localization of ssap in s. schlegeli was investigated by reverse transcription (rt)-pcr, ... | 2007 | 17964810 |
| an entd-like phosphopantetheinyl transferase gene from photobacterium profundum ss9 complements pfa genes of moritella marina strain mp-1 involved in biosynthesis of docosahexaenoic acid. | the entd-like phosphopantetheinyl transferase (pptase) gene, cloned from the eicosapentaenoic acid-producing bacterium photobacterium profundum strain ss9, has an orf of 690 bp encoding a 230-amino acid protein. when this pptase gene was expressed in escherichia coli with pfaa, pfab, pfac and pfad derived from moritella marina mp-1, which were four of five essential genes for biosynthesis of docosahexaenoic acid (dha), the dha production of the recombinant was 2% (w/w) of total fatty acids. this ... | 2008 | 17973084 |
| photobacterium sp. jt-ish-224 produces two sialyltransferases, alpha-/beta-galactoside alpha2,3-sialyltransferase and beta-galactoside alpha2,6-sialyltransferase. | a novel bacterium, photobacterium sp. jt-ish-224, that produces alpha-/beta-galactoside alpha2,3-sialyltransferase and beta-galactoside alpha2,6-sialyltransferase, was isolated from the gut of a japanese barracuda. the genes that encode the enzymes were cloned from the genomic library of the bacterium using the genes encoding alpha-/beta-galactoside alpha2,3-sialyltransferase from p. phosphoreum and beta-galactoside alpha2,6-sialyltransferase from p. damselae as probes. the nucleotide sequences ... | 2008 | 17984122 |
| n-terminal 112 amino acid residues are not required for the sialyltransferase activity of photobacterium damsela alpha2,6-sialyltransferase. | photobacterium damsela alpha2,6-sialyltransferase was cloned as n- and c- his-tagged fusion proteins with different lengths (16-497 aa or 113-497 aa). expression and activity assays indicated that the n-terminal 112 amino acid residues of the protein were not required for its alpha2,6-sialyltransferase activity. among four truncated forms tested, n-his-tagged delta15pd2,6st(n) containing 16-497 amino acid residues had the highest expression level. similar to the delta15pd2,6st(n), the shorter de ... | 2008 | 17989925 |
| lumazine proteins from photobacteria: localization of the single ligand binding site to the n-terminal domain. | lumazine protein is believed to serve as an optical transponder in bioluminescence emission by certain marine bacteria. sequence arguments suggest that the protein comprises two similarly folded riboflavin synthase-type domains, but earlier work also suggested that only one domain binds 6,7-dimethyl-8-ribityllumazine (dmrl). we show that the replacement of serine-48 or threonine-50 in the n-terminal domain of lumazine protein of photobacterium leiognathi modulates the absorbance and fluorescence ... | 2007 | 18020947 |
| marine bacteria comprise a possible indicator of drowning in seawater. | to investigate the effectiveness of marine bacteria as a new marker of drowning in seawater, we determined the optimal conditions of media required to selectively detect marine bacteria and applied the technique to drowned cadavers. we incubated model blood samples (n=20 per group) mixed with seawater, river, tap or muddy water on agar plates (todd hewitt, th; marine 2216, m2216) and determined the nacl concentration required to selectively detect marine bacteria. we also used tcbs agar plates w ... | 2008 | 18023311 |
| quality changes during refrigerated storage of ma-packaged pre-rigor fillets of farmed atlantic cod (gadus morhua l.) using traditional map, co2 emitter, and vacuum. | quality changes during 3 wk of refrigerated storage (1.3 degrees c) were studied on pre-rigor filleted farmed atlantic cod packed in modified atmosphere (map, 60% co2 and 40% o2) or vacuum. the packages of map contained either a co2 emitter and low gas volume to product volume (g/p ratio) of 1.3, or a 3.9 g/p ratio and no emitter. the co2 level remained stable or increased in the packages with co2 emitter, whereas the co2 level in the packages with no co2 emitter decreased to 40% after 4 d of re ... | 2007 | 18034737 |
| microflora assessments using pcr-denaturing gradient gel electrophoresis of ozone-treated and modified-atmosphere-packaged farmed cod fillets. | denaturing gradient gel electrophoresis (dgge) of a pcr-amplified 16s rdna sequence was used to characterize changes in the microbial flora caused by ozone (o3) treatment of farmed cod (gadus morhua). portions of cod were produced under controlled conditions, bathed in fresh water supplemented with 2 ppm of o3 for 30 min, and packaged in modified atmosphere (ma: 60% co2 and 40% n2) before 4 degrees c storage. control samples were packaged in ma or air, without prior o3 treatment. samples were an ... | 2007 | 18044421 |
| reclassification of vibrio fischeri, vibrio logei, vibrio salmonicida and vibrio wodanis as aliivibrio fischeri gen. nov., comb. nov., aliivibrio logei comb. nov., aliivibrio salmonicida comb. nov. and aliivibrio wodanis comb. nov. | four closely related species, vibrio fischeri, vibrio logei, vibrio salmonicida and vibrio wodanis, form a clade within the family vibrionaceae; the taxonomic status and phylogenetic position of this clade have remained ambiguous for many years. to resolve this ambiguity, we tested these species against other species of the vibrionaceae for phylogenetic and phenotypic differences. sequence identities for the 16s rrna gene were > or =97.4 % among members of the v. fischeri group, but were < or =9 ... | 2007 | 18048732 |
| antibacterial action of l-amino acid oxidase from the skin mucus of rockfish sebastes schlegelii. | l-amino acid oxidase (lao) shows broadly antibacterial activity against gram-positive and gram-negative bacteria by h(2)o(2) generated in the oxidative process of l-amino acids. however, lao (termed ssap) isolated from the rockfish sebastes schlegelii skin mucus acted selectively on gram-negative bacteria. therefore, this study was undertaken to clarify the antibacterial action of ssap as compared with h(2)o(2). ssap inhibited potently the growth of aeromonas salmonicida, photobacterium damselae ... | 2008 | 18055243 |
| isolation of photobacterium damselae subsp. piscicida from diseased tongue sole (cynoglossus semilaevis gunther) in china. | during the summer of 2006, an epizootic occurred among cultured tongue sole (cynoglossus semilaevis gunther) in a fish farm in qingdao, china. the diseased tongue sole exhibited haemorrhaging of the basal fin, yellowed kidney and ulceration of the body surface. a gram-negative,rod shaped bacterium (designated strain wy06) was isolated from the gall bladder of diseased fish. pathogenicity assays revealed that wy06 was virulent to tongue sole and zebrafish (danio rerio) by intraperitoneal injectio ... | 2007 | 18062245 |
| genes encoding the vibrio harveyi haemolysin (vhh)/thermolabile haemolysin (tlh) are widespread in vibrios. | v. harveyi vhh haemolysin, which shows high homology to the tlh haemolysin (the identities of their deduced amino acid sequences are up to 85.6%), is a putative virulence factor to marine cultured fish. a vhh probe, which is specific to v. harveyi vhha haemolysin gene, was used to screen ecor i digests of total dna from 57 vibrio strains, including 26 vibrio type strains, 20 v. harveyi isolates and 11 v. parahaemolyticus isolates. as a result, 1 strong hybridisation band was detected in 13 type ... | 2007 | 18062266 |
| toxicity and biodegradability of imidazolium ionic liquids. | several bioassays have been carried out to analyze the toxicity and biodegradability of several imidazolium ionic liquids (ils) in aqueous phase. the synthetized compounds consist of an imidazolium cation with two alkyl substituents in positions 3 (r1) and 1 (r2) and a counter-ion. the alkyl substituent r1 has been fixed as a methyl group and the effect of the alkyl chain length (c1-c8) of the other substituent (r2) has been tested. moreover, the influence of diverse counter-ions a- (cl-, pf6, x ... | 2008 | 18063302 |
| luminous bacteria as potential producers of resorbed polyhydroxyalkanoate polyesters. | | 2007 | 18064824 |
| complete dna sequence and analysis of the transferable multiple-drug resistance plasmids (r plasmids) from photobacterium damselae subsp. piscicida isolates collected in japan and the united states. | photobacterium damselae subsp. piscicida is a bacterial fish pathogen that causes a disease known as pasteurellosis. two transferable multiple-drug resistance (r) plasmids, pp99-018 (carrying resistance to kanamycin, chloramphenicol, tetracycline, and sulfonamide) and pp91278 (carrying resistance to tetracycline, trimethoprim, and sulfonamide), isolated from p. damselae subsp. piscicida strains from japan (p99-018) and the united states (p91278), respectively, were completely sequenced and analy ... | 2008 | 18070959 |
| co-occurrence of viral and bacterial pathogens in disease outbreaks affecting newly cultured sparid fish. | several microbial disease outbreaks in farm stocks of newly cultured sparid fish species, such as common seabream, redbanded seabream, and white seabream, were recorded from 2004 to 2006. this study describes the isolation and characterization of the potential causative agents, either bacteria or viruses, of these outbreaks. the isolated bacterial strains were characterized according to traditional taxonomical analyses and sequencing of a 16s rdna fragment. most bacteria were identified as vibri ... | 2007 | 18076001 |
| susceptibility of photobacterium damselae subsp. piscicida strains isolated from senegalese sole, solea senegalensis kaup, and gilthead seabream, sparus aurata l., to several antibacterial agents. | | 2008 | 18086037 |
| investigation of sewage sludge stabilization potential by the addition of fly ash and lime. | the aim of this work was the examination of stabilization potential of sewage sludge by the addition of fly ash and/or lime and the investigation of the effect of stabilization time on the properties of produced mixtures. five samples were prepared by mixing fly ash, sewage sludge and lime in various ratios and the mixtures were stabilized for a period of 35 d. the addition of alkaline agents resulted in the increase of sample ph up to 12, the increase of total solids content to about 50% and th ... | 2008 | 18093729 |
| bioreactivity of municipal solid waste landfill leachates-hormesis and dna damage. | the issue of domestic waste is recognised as one of the most serious environmental problems facing the nation. with the uk producing 35 million tonnes of municipal solid waste per annum, an understanding of the ranges of toxicity of landfill emissions is crucial to determine the degree of concern we should have about the potential effects these waste sites could have upon nearby populations and the surrounding environment. the aim of this study was to evaluate the bioreactivity of landfill leach ... | 2008 | 18155125 |
| luxg is a functioning flavin reductase for bacterial luminescence. | the luxg gene is part of the lux operon of marine luminous bacteria. luxg has been proposed to be a flavin reductase that supplies reduced flavin mononucleotide (fmn) for bacterial luminescence. however, this role has never been established because the gene product has not been successfully expressed and characterized. in this study, luxg from photobacterium leiognathi th1 was cloned and expressed in escherichia coli in both native and c-terminal his6-tagged forms. sequence analysis indicates th ... | 2008 | 18156264 |
| large-scale transposon mutagenesis of photobacterium profundum ss9 reveals new genetic loci important for growth at low temperature and high pressure. | microorganisms adapted to piezopsychrophilic growth dominate the majority of the biosphere that is at relatively constant low temperatures and high pressures, but the genetic bases for the adaptations are largely unknown. here we report the use of transposon mutagenesis with the deep-sea bacterium photobacterium profundum strain ss9 to isolate dozens of mutant strains whose growth is impaired at low temperature and/or whose growth is altered as a function of hydrostatic pressure. in many cases t ... | 2008 | 18156275 |
| total substitution of fish oil by vegetable oils in gilthead sea bream (sparus aurata) diets: effects on hepatic mx expression and some immune parameters. | the use of vegetable oils in fish nutrition has been extensively studied; and recent work has focused attention on replacing fish oil with alternative fatty acid sources and their effect on the immune system. however, little is known about the effect of these oils on immune parameters such as the fish interferon system. in this study we evaluate the effect of two vegetable oils (linseed and soybean) on gilthead sea bream mx expression and other innate immune parameters. experimental diets were f ... | 2008 | 18158252 |
| the dimeric assembly of photobacterium leiognathi and salmonella typhimurium sodc1 cu,zn superoxide dismutases is affected differently by active site demetallation and ph: an analytical ultracentrifuge study. | to establish whether the species-specific variations at the subunit interface of bacterial cu,zn superoxide dismutases affect dimer assembly, the association state of the photobacterium leiognathi (plsod) and salmonella typhimurium (stsod) enzymes, which differ in 11 out of 19 interface residues, was investigated by analytical ultracentrifugation. the same linkage pattern correlates quaternary assembly, active site metallation, and ph in the two enzymes albeit with quantitative differences. both ... | 2008 | 18179768 |
| necrotizing fasciitis of the leg due to photobacterium damsela. | | 2008 | 18181778 |
| structural basis for the cold adaptation of psychrophilic m37 lipase from photobacterium lipolyticum. | the m37 lipase from photobacterium lipolyticum shows an extremely low activation energy and strong activity at low temperatures, with optimum activity seen at 298 k and more than 75% of the optimum activity retained down to 278 k. though the m37 lipase is most closely related to the filamentous fungal lipase, rhizomucor miehei lipase (rml) at the primary structure level, their activity characteristics are completely different. in an effort to identify structural components of cold adaptation in ... | 2008 | 18186467 |
| microbial contamination of dental unit waterlines in istanbul, turkey. | the water used in dental unit waterlines (duwls) acts as a coolant for the high-speed equipment and as an irrigant during dental treatments. there are kind of water tanks. duwls provide a favorable environment for microbial biofilm and multiplation primarily due to the high surface in the tubing and the character of fluid dynamics in narrow, smooth-walled waterlines. biofilms can harbour opportunist pathogens such as legionella sp., pseudomonas sp. several studies have shown that duwls have high ... | 2008 | 18210208 |
| defences against oxidative stress in vibrios associated with corals. | bacteria colonizing healthy coral tissue may produce enzymes capable of overcoming the toxic effects of reactive oxygen species, including superoxide dismutase (sod) and catalase. significant differences in the activities of these enzymes were observed in cultures of vibrio campbellii, vibrio coralliilyticus, vibrio harveyi, vibrio mediterranei, vibrio pelagius, vibrio rotiferanus, vibrio tasmaniensis, and photobacterium eurosenbergii isolated from healthy, bleached or necrotic tropical and cold ... | 2008 | 18279336 |
| culturable heterotrophic bacteria in seawater and mytilus galloprovincialis from a mediterranean area (northern ionian sea-italy). | although mar piccolo of taranto (ionian sea, italy) is one of the most important mytilus galloprovincialis farming areas, data concerning the natural bacterial microbiota of these mussels and their surrounding environment are still scant. this study was carried out seasonally, throughout a year, to determine culturable heterotrophic bacteria both in the water and mussels samples collected at three sampling sites in the northern ionian sea: s. vito, lido gandoli and lido silvana. culturable heter ... | 2009 | 18301997 |
| unnarmicins a and c, new antibacterial depsipeptides produced by marine bacterium photobacterium sp. mbic06485. | two new antibiotic depsipeptides, unnarmicins c (1) and a (2), were isolated from the fermentation broth of a marine bacterium, photobacterium sp. strain mbic06485. the structure of 1 was established by spectroscopic studies and chiral analyses of its chemical degradation/conversion products, and that of 2 by comparing its nmr, ms, and cd data with those of 1. both compounds selectively inhibited the growth of two strains belonging to the genus pseudovibrio, one of the most prevalent genera in t ... | 2008 | 18305354 |
| vaccination of larvae of the bony fish gilthead seabream reveals a lack of correlation between lymphocyte development and adaptive immunocompetence. | fish eggs are released and embryos hatch into a pathogenically hostile environment, at a time when their immunological capacity is severely limited. although the eggs are initially protected by the envelope as well as by several innate and adaptive immune substances, which are transferred to eggs during fish vitellogenesis, it seems that young specimens depend fundamentally on their innate defence mechanisms. here we show in the gilthead seabream, an immunologically tractable teleost fish model, ... | 2008 | 18325592 |
| genomic and functional analysis of icepdaspa1, a fish-pathogen-derived sxt-related integrating conjugative element that can mobilize a virulence plasmid. | integrating conjugative elements (ices) are self-transmissible mobile elements that transfer between bacteria via conjugation and integrate into the host chromosome. sxt and related ices became prevalent in asian vibrio cholerae populations in the 1990s and play an important role in the dissemination of antibiotic resistance genes in v. cholerae. here, we carried out genomic and functional analyses of icepdaspa1, an sxt-related ice derived from a spanish isolate of photobacterium damselae subsp. ... | 2008 | 18326579 |
| observation of oscillation in bacterial luminescence. | oscillation in the bioluminescent intensity from a luminous bacteria suspension was observed. the time course of the luminescence intensity from a suspension containing luminous bacteria was measured. the oscillation mode changed with the liquid broth component. the optical density and dissolved oxygen (do) concentration were measured simultaneously with the luminescence intensity, and a possibility was indicated that both diauxic growth and oxygen reaction-consumption resulted with oscillation. | 2008 | 18332555 |
| three cases of gastric prolapse through the gill slit in sand tiger sharks, carcharhinus taurus (rafinesque). | | 2008 | 18353023 |
| the efficacy and safety of an oil-based vaccine against photobacterium damsela subsp. piscicida in yellowtail (seriola quinqueradiata): a field study. | protection after intraperitoneal (i.p.) vaccination of yellowtail (seriola quinqueradiata) against pasteurellosis was studied in a field trial. yellowtail juveniles captured from the wild or artificially hatched were immunised with an oil-based vaccine against photobacterium damsela subsp. piscicida, and the fish were observed for 15 weeks after vaccination. outbreak of pasteurellosis was observed at all five sites (mortality in control group ranged from 7% to 77%), and significant (p<0.01) prot ... | 2008 | 18356079 |
| phylogenetic analysis of the incidence of lux gene horizontal transfer in vibrionaceae. | horizontal gene transfer (hgt) is thought to occur frequently in bacteria in nature and to play an important role in bacterial evolution, contributing to the formation of new species. to gain insight into the frequency of hgt in vibrionaceae and its possible impact on speciation, we assessed the incidence of interspecies transfer of the lux genes (luxcdabeg), which encode proteins involved in luminescence, a distinctive phenotype. three hundred three luminous strains, most of which were recently ... | 2008 | 18359809 |
| shewanella and photobacterium spp. in oysters and seawater from the delaware bay. | shewanella algae, s. putrefaciens, and photobacterium damselae subsp. damselae are indigenous marine bacteria and human pathogens causing cellulitis, necrotizing fasciitis, abscesses, septicemia, and death. infections are rare and are most often associated with the immunocompromised host. a study was performed on the microbiological flora of oysters and seawater from commercial oyster harvesting sites in the delaware bay, new jersey. from 276 water and shellfish samples tested, 1,421 bacterial i ... | 2008 | 18378645 |
| fate and effects of amphoteric surfactants in the aquatic environment. | amphoteric surfactants form part of specialty surfactants available for formulators to improve or design new formulations in response to environmental, toxicity, safety and performance demands. nevertheless, limited information on the ecological properties of amphoterics is available. in the present work, the aerobic and anaerobic biodegradability and the aquatic toxicity of different types of amphoteric surfactants (three alkyl betaines, one alkylamido betaine and three alkyl imidazoline deriva ... | 2008 | 18456334 |
| evolutionary ecology during the rise of dioxygen in the earth's atmosphere. | pre-photosynthetic niches were meagre with a productivity of much less than 10(-4) of modern photosynthesis. serpentinization, arc volcanism and ridge-axis volcanism reliably provided h(2). methanogens and acetogens reacted co(2) with h(2) to obtain energy and make organic matter. these skills pre-adapted a bacterium for anoxygenic photosynthesis, probably starting with h(2) in lieu of an oxygen 'acceptor'. use of ferrous iron and sulphide followed as abundant oxygen acceptors, allowing producti ... | 2008 | 18468980 |
| molecular characterization, phylogeny, and expression of c-type and g-type lysozymes in brill (scophthalmus rhombus). | lysozymes are key proteins of the innate immune system against bacterial infections. in this study we report the molecular cloning and characterization of the c-type and g-type lysozymes in brill (scophthalmus rhombus). catalytic and other conserved residues required for functionality were identified. phylogenetic analysis revealed distinct evolutionary histories for each lysozyme type. expression profiles of both lysozyme genes were studied in juvenile tissues using a real-time pcr approach. c- ... | 2008 | 18479938 |
| identification of a cellobiose utilization gene cluster with cryptic beta-galactosidase activity in vibrio fischeri. | cellobiose utilization is a variable trait that is often used to differentiate members of the family vibrionaceae. we investigated how vibrio fischeri es114 utilizes cellobiose and found a cluster of genes required for growth on this beta-1,4-linked glucose disaccharide. this cluster includes genes annotated as a phosphotransferase system ii (cela, celb, and celc), a glucokinase (celk), and a glucosidase (celg). directly downstream of celcbgka is celi, which encodes a laci family regulator that ... | 2008 | 18487409 |
| in vivo morphological and antigenic characteristics of photobacterium damselae subsp. piscicida. | the present study was conducted to examine the morphology and antigenicity of photobacterium damselae subsp. piscicida by culturing the bacterium in vivo in the peritoneal cavity of sea bass (dicentrarchus labrax) within dialysis bags with either a low molecular weight (lmw) cut-off of 25 kda or a high molecular weight (hmw) cut-off of 300 kda. differences were observed in the growth rate between the bacteria cultured in vivo or in vitro. bacteria cultured in vivo were smaller and produced a cap ... | 2008 | 18487938 |
| difference of genotypic and phenotypic characteristics and pathogenicity potential of photobacterium damselae subsp. damselae between clinical and environmental isolates from japan. | photobacterium damselae subsp. damselae has been known as an opportunistic pathogen in fish and mammals. human infectious cases are often very serious and occasionally fatal. we previously reportedtwo fatal cases caused by this subspecies where the patients developed multiple organ failure within 20-36 h after the onset of initial symptoms. despite its ability to cause serious infections in humans, this subspecies has not been well studied because human infectious cases caused by this subspecies ... | 2008 | 18554860 |
| prepared bed bioremediation of oily sludge in an oilfield in northern china. | field-scale bioremediation of oily sludge in prepared beds was studied at shengli oilfield in northern china. the influence of manure, coarse sand, sawdust, a specialized microbial preparation and greenhouse conditions on the efficiency of removal of oil and grease was evaluated. after bioremediation for 230d, oil and grease content fell by 32-42gkg(-1)dry sludge in treated plots, indicating removal of 27-46% compared with only 15% in the control plot. addition of manure, coarse sand, sawdust an ... | 2009 | 18572312 |
| effect of dietary administration of porphyridium cruentum on the respiratory burst activity of sole, solea senegalensis (kaup), phagocytes. | the stimulatory effect of the red microalga porphyridium cruentum on respiratory burst activity of sole phagocytes was evaluated in vivo. oral administration of a diet supplemented with lyophilized p. cruentum cells (10 g kg(-1)) stimulated respiratory burst activity after 4 weeks feeding in sole vaccinated with photobacterium damselae subsp. piscicida bacterin. | 2008 | 18577098 |
| the crosslinking and antimicrobial properties of tunichrome. | tunichromes are small peptides containing one or more dehydrodopa derived units that have been identified in the blood cells of at least eleven species of tunicates. incubation of tunichromes isolated from ascidia nigra hemocytes (or model dopa-containing compounds) under oxidative conditions with either lysozyme, cytochrome c or ovalbumin resulted in a time-dependent polymerization of these test proteins to dimers, trimers, tetramers and potentially to other oligomers. these results indicate th ... | 2008 | 18593604 |
| a novel bod sensor based on bacterial luminescence. | a reagent-type bod sensor with a new principle employing a luminous bacterium, photobacterium phosphoreum, was developed. the increased intensity of luminescence resulting from the cellular assimilation of organic compounds in wastewater was detected by a photodiode. the bod response of the bacterial reagent could be obtained within 15 min with +/-7% error. the temperature condition for optimal bod response was 18 degrees to 25 degrees c at ph 7 to 8, indicating that it is possible to measure bo ... | 1993 | 18601297 |
| structure of lumazine protein, an optical transponder of luminescent bacteria. | the intensely fluorescent lumazine protein is believed to be involved in the bioluminescence of certain marine bacteria. the sequence of the catalytically inactive protein resembles that of the enzyme riboflavin synthase. its non-covalently bound fluorophore, 6,7-dimethyl-8-ribityllumazine, is the substrate of this enzyme and also the committed precursor of vitamin b(2). an extensive crystallization screen was performed using numerous single-site mutants of the lumazine protein from photobacteri ... | 2008 | 18602927 |
| [research of a bioluminent bacterial-based optical fiber sensor to detecting acute effects of pollutants in water]. | field detection of general toxicity is urgent demand in these years, therefore rapid, sensitive, convenient biosensor was studied to detect cute toxicity of water pollutant, which is a novel optical fiber sensor immobilized bioluminent bacterial, photobacterium phosphorem t3. this bioluminence light was measured by light detection system after it coupling to optical fiber. for the activity and bioluminence of bacterial was seriously affected by immobilizing conditions, optimization of immobiliza ... | 2008 | 18613508 |
| [diuron degradation process and change of toxicity by gamma-irradiation]. | the radiation-induced degradation process of diuron and change of toxicity by gamma-irradiation were investigated. the contribution to diuron degradation by the radicals is in the order of: *oh > e(aq)(-) > *h. the quantum efficiency ratios of *oh, e(aq)(-) and *h for the degradation of diuron are calculated as 3 : 1 : 2. e(aq)(-) and *h could reduce diuron and its degradation product by loss of the chlorine atoms and the addition reaction. both the shift and alkaline substitute mutations firstl ... | 2008 | 18624209 |
| [a study on toxicity of aquaculture drugs to luminescent bacteria]. | to study the dose-response relation of the fish drugs and their joint toxicity on the luminescent bacteria. | 2008 | 18646524 |
| comparative study of phenol and cyanide containing wastewater in cstr and sbr activated sludge reactors. | the objectives of this work were the examination of the performance of two bench scale activated sludge systems, a conventional continuous stirring tank reactor (cstr) and a sequential batch reactor (sbr), for the treatment of wastewaters containing phenol and cyanides and the assessment of the toxicity reduction potential by bioassays. the operation of the reactors was monitored by physicochemical analyses, while detoxification potential of the systems was monitored by two bioassays, the marine ... | 2009 | 18650084 |
| [effect of salts on luminescence of natural and recombinant luminescent bacterial biosensors]. | effect of cations k+, na+, mg2+, and ca2+ and anions so4(2-), hco3(-), and co3(2-) on the luminescence intensity of the marine luminescent bacterium photobacterium phorphoreum (microbiosensor b-17 677f) and the recombinant strain escherichia coli with cloned lux operon of p. leiognathi (ekolyum-9). it is found that small concentrations of chlorides and sulfates of the cations studied had a concentration-dependent stimulatory effect on bacterial bioluminescence; as the concentration of agents inc ... | 2008 | 18663957 |
| location of lectin exhibiting specificity for n-acetyl-d-galactosamine in cells of the symbiotic marine bacteria photobacterium phosphoreum. | | 2008 | 18680915 |
| [synthesis of reserve polyhydroxyalkanoates by luminescent bacteria]. | the ability of marine luminescent bacteria to synthesize polyesters of hydroxycarboxylic acids (polyhydroxyalkanoates, pha) as reserve macromolecules was studied. twenty strains from the collection of the luminescent bacteria ccibso (wdsm839) of the institute of biophysics, siberian branch, russian academy of sciences, assigned to different taxa (photobacterium leiognathi, ph. phosphoreum, vibrio harveyi, and v. fischeri) were analyzed. the most productive strains were identified, and the condit ... | 2008 | 18683654 |
| the deep-sea bacterium photobacterium profundum ss9 utilizes separate flagellar systems for swimming and swarming under high-pressure conditions. | motility is a critical function needed for nutrient acquisition, biofilm formation, and the avoidance of harmful chemicals and predators. flagellar motility is one of the most pressure-sensitive cellular processes in mesophilic bacteria; therefore, it is ecologically relevant to determine how deep-sea microbes have adapted their motility systems for functionality at depth. in this study, the motility of the deep-sea piezophilic bacterium photobacterium profundum ss9 was investigated and compared ... | 2008 | 18723648 |
| gammaproteobacteria as a possible source of eicosapentaenoic acid in anoxic intertidal sediments. | eicosapentaenoic acid (epa; n-20:5omega3) was found to be a constituent of phospholipids in three mesophilic strains of gammaproteobacteria, which were isolated from anoxic most probable number series prepared with sediments from an intertidal flat of the german north sea coast. their partial 16s rrna gene sequences identified the isolates as close relatives of shewanella colwelliana, vibrio splendidus, and photobacterium lipolyticum. so far, eicosapentaenoic acid has mainly been reported to occ ... | 2009 | 18777187 |
| surveillance of health status on eight marine rainbow trout, oncorhynchus mykiss (walbaum), farms in denmark in 2006. | the health status of eight marine rainbow trout farms was followed from mid-june to mid-september 2006 by sampling both dead and healthy fish approximately every 2 weeks for bacteriological and virological investigation. no fish pathogenic viruses were detected, but all farms experienced disease and mortality as a result of various bacterial infections. yersinia ruckeri was found on four and renibacterium salmoninarum on five of the farms, but only during the first part of the surveillance perio ... | 2008 | 18786028 |
| c-lysozyme from senegalese sole (solea senegalensis): cdna cloning and expression pattern. | lysozymes are key molecules of innate immunity and proved high bactericidal activity in fish, thus becoming attractive as tools for enhancing fish defences. in this study, a full-length c-type lysozyme cdna from senegalese sole (solea senegalensis) has been cloned and characterized. the cdna sequence was inferred from two overlapping fragments obtained by race-pcr and consisting on 631bp coding for 143 aminoacids. catalytic and other conserved residues required for lysozyme activity were identif ... | 2008 | 18786641 |
| study on the synergic effect of natural compounds on the microbial quality decay of packed fish hamburger. | the effectiveness of natural compounds in slowing down the microbial quality decay of refrigerated fish hamburger is addressed in this study. in particular, the control of the microbiological spoilage by combined use of three antimicrobials, and the determination of their optimal composition to extend the fish hamburger microbiological stability limit (mal) are the main objectives of this work. thymol, grapefruit seed extract (gfse) and lemon extract were tested for monitoring the cell growth of ... | 2008 | 18804302 |
| [toxicity screening and identification of bacteria isolated from snails nassarius semiplicatus and their habitat]. | tetrodotoxin and its analogues (ttxs) were responsible for the poisoning incidents associated with snail nassarius spp. we studied bacteria isolated from toxic snails as well as their habitat to probe into the relationship between bacteria and toxicity of nassariid gastropod. | 2008 | 18837369 |
| photocatalytic disinfection of marine bacteria using fluorescent light. | photocatalytic oxidation (pco) using fluorescent light was used to disinfect two marine bacteria: alteromonas alvinellae and photobacterium phosphoreum. results showed that p. phosphoreum exhibited a lower susceptibility towards pco than a. alvinellae, which was related to their fatty acid profiles and levels of superoxide dismutase (sod) and catalase (cat). results also revealed that cat and sod levels would be triggered by pco reaction. in addition, pco disinfection efficiency could be enhance ... | 2008 | 18842281 |
| [biotests for mineral waters with natural and recombinant luminescent microorganisms]. | we have developed methods of biotesting mineral waters involving use of natural or recombinant luminescent strains with elimination of the effect of salt concentration and ph. to overcome the adverse effect of high salt concentrations, disguising the action of chemical pollutants, a special method of mineral water sample preparation is proposed. in this method, the marine luminescent bacterium photobacterium phosphoreum (microbiosensor b17 677f) is used as a test object. samples to be analyzed a ... | 2008 | 18924408 |
| [quantitative criteria for the estimation of the effectiveness of bioluminescence expression in natural and transgenic luminescent bacteria]. | computation coefficients for estimating the effectiveness of bioluminescence expression in natural luminescent bacteria p. leiognathi 54 and transgenic strain e. coli z905/pphl7 bearing lux-operon in multicopy plasmid are suggested, and their use on the molecular, cell, and population levels was considered. it was shown that, on the population level, all transgenic variants got the better of natural variants of p. leiognathi 54 irrespective of the type of lux-operon regulation. on the cell level ... | 2008 | 18954013 |
| biodegradability and toxicity of sulphonate-based surfactants in aerobic and anaerobic aquatic environments. | four types of commonly used sulphonate-based surfactants (alkane sulphonates, alpha-olefin sulphonates, sulphosuccinates and methyl ester sulphonates) were tested for their aerobic and anaerobic biodegradability as well as for their toxicity to daphnia magna and photobacterium phosphoreum to assess the effect of the surfactant structure on those properties. aerobic biodegradation was evaluated by means of the co2 headspace test and anaerobic biodegradation was assessed by a method based on the e ... | 2009 | 18976786 |
| developmental and microbiological analysis of the inception of bioluminescent symbiosis in the marine fish nuchequula nuchalis (perciformes: leiognathidae). | many marine fish harbor luminous bacteria as bioluminescent symbionts. despite the diversity, abundance, and ecological importance of these fish and their apparent dependence on luminous bacteria for survival and reproduction, little is known about developmental and microbiological events surrounding the inception of their symbioses. to gain insight on these issues, we examined wild-caught larvae of the leiognathid fish nuchequula nuchalis, a species that harbors photobacterium leiognathi as its ... | 2008 | 18978090 |
| 15n metabolic test for the determination of phytotoxic effects of chemicals and contaminated environmental samples. | a stable isotope(15)n-nitrogen test (esima = ecotoxicological stable isotope metabolic assay) was developed to assess biological effects and the potential toxicological hazard of chemicals and contaminated environmental samples on plant metabolism. the assay measures the effect of toxicants on the incorporation of a(15)n labelled tracer into the total nitrogen fraction (both the nonprotein and protein fraction) of plants. segments of pisum arvense epicotyls are used as test substrates because of ... | 1999 | 19009406 |
| protein evolution in deep sea bacteria: an analysis of amino acids substitution rates. | abyssal microorganisms have evolved particular features that enable them to grow in their extreme habitat. genes belonging to specific functional categories are known to be particularly susceptible to high-pressure; therefore, they should show some evidence of positive selection. to verify this hypothesis we computed the amino acid substitution rates between two deep-sea microorganisms, photobacterium profundum ss9 and shewanella benthica kt99, and their respective shallow water relatives. | 2008 | 19014525 |
| observations on the estrogenic activity and concentration of 17beta-estradiol in the discharges of 12 wastewater treatment plants in southern australia. | there is very little information on the overall level of estrogenic activity, or concentrations of specific hormonal compounds in wastewater treatment plant (wwtp) discharges in australia, compared with europe, japan, and north america. to partly address this, in 2004, water samples were collected as "grab" or "spot" samples from 12 wwtp facilities across southern victoria at the point at which effluent enters the environment, either as recycled water or direct discharge to the receiving water. ... | 2009 | 19057835 |
| [adaptation of bioluminescence technique for the evaluation of the toxicity of mineral waters]. | mineral waters have been ascertained to exert a pronounced inhibiting effect, that is in proportion in their mineralization and ph values, on the fluorescence of microbial luminescent biosensors. to prevent such adverse reactions masking the effects of chemical pollutants, the authors propose an adjusted procedure for preparing the samples of mineral waters for luminescence biotesting. the first approach is to differentially insert additional quantities of nacl into the test samples depending on ... | 2008 | 19097443 |
| effect of quinone on the fluorescence decay dynamics of endogenous flavin bound to bacterial luciferase. | the interaction of quinone with luciferase from photobacterium leiognathi was studied based on the fluorescence decay measurements of the endogenous flavin bound to the enzyme. homologous 1,4-quinones, 1,4-benzoquinone, methyl-1,4-benzoquinone, 2-methyl-5-isopropyl-1,4-benzoquine and 1,4-naphthoquinone, were investigated. in the absence of quinone, the fluorescence intensity and anisotropy decays of the endogenous flavin exhibited two intensity decay lifetimes (~1 and 5 ns) and two anisotropy de ... | 2009 | 19162392 |
| [construction of recombinate luminescence bacteria vector to evaluate the genetoxic of environment pollutant]. | recombinate luminescence bacteria have the important role in evaluating water toxicity. two recombinate luminescence bacteria vectors pucd-uvra and pucd-alka were constructed to investigate the impaired mechanism of pollutant genetic toxicity. the genes of uvra and alka were amplified by pcr from e. coli w3110, sequenced after ligated with pgem-t easy vector. the pcr products and pucd615 vector were all digested with bamh i, ecor i, then be linked and imported into jm109 with electrotransformati ... | 2008 | 19186821 |
| comparison between the short-term and the long-term toxicity of six triazine herbicides on photobacteria q67. | the bioluminescence inhibition of six triazine herbicides including desmetryne (des), simetryn (sim), velpar (vel), prometon (pro), metribuzin (met), and aminotriazine (ami) on vibrio qinghaiensis sp.-q67 (q67) was determined to investigate the effects of exposure duration on the ecotoxicological relevance of triazine herbicides. based on the short-term microplate toxicity analysis (mta), a long-term mta was established to assess the impact of exposure time on the toxicities of the herbicides. t ... | 2009 | 19203776 |
| bioluminescence intensity difference observed in luminous bacteria groups with different motility. | the aim of this study was to compare the luminescent intensity of bioluminescence from marine luminous bacteria with different motility. | 2009 | 19207857 |
| combined photobacterium toxicity of herbicide mixtures containing one insecticide. | to test whether the dose-addition (da) model can predict the combined toxicity of the mixtures of herbicides that coexisted with insecticide(s), we selected five herbicides (simetryn, prometon, bromacil, velpar, and diquat) and one organophosphorus insecticide (dichlorvos) as the test components. the inhibition toxicities of the six pesticides as well as those of their mixtures to vibrio qinghaiensis sp.-q67 were determined by using the microplate toxicity test procedure. the dose-response curve ... | 2009 | 19215957 |
| detection of bacterioplankton in immersed cadavers using selective agar plates. | we measured bacterioplankton in blood from cadavers retrieved from the sea (n=12), near estuaries (n=4), rivers (fresh water, n=8) and from bathtubs (n=4) as well as from non-drowned victims (n=10) discovered near aquatic environments. blood from 11 victims drowned in seawater developed bioluminescent and/or blue colonies (oxidase test positive) on selective media containing 2-4% nacl. homology analyses of the 16s rrna gene showed that all of them were marine bacteria (genera: photobacterium, vi ... | 2009 | 19261520 |
| evaluation of the ecotoxicity of model nanoparticles. | since society at large became aware of the use of nanomaterials in ever growing quantities in consumer products and their presence in the environment, critical interest in the impact of this emerging technology has grown. the main concern is whether the unknown risks of engineered nanoparticles (nps), in particular their impact on health and environment, outweighs their established benefits for society. therefore, a key issue in this field is to evaluate their potential toxicity. in this context ... | 2009 | 19264345 |
| piscidin 4, a novel member of the piscidin family of antimicrobial peptides. | piscidins are linear, amphipathic, antimicrobial peptides (amps) with broad, potent, activity spectrum. piscidins and other members of the piscidin family appear to comprise the most common group of amps in teleost fish. all piscidins and related members of the piscidin family described to date are 18-26 amino acids long.we report here the isolation of a novel 5329.25 da, 44-residue (ffrhlfrgakaifrgarqgxrahkvvsryrnrdvpetdnnqeep) antimicrobial peptide from hybrid striped bass (morone chrysops fem ... | 2009 | 19266617 |
| photobacterium damsela wound infection in a 14-year-old surfer. | photobacterium damsela (p damsela) is a common marine bacterium. rare reports of p damsela causing septicemia and wound infection have been described in children. we report a case of photobacterium damsela isolated from a wound culture taken from a 14-year-old male injured after a minor trauma while surfing. we alert physicians to this infection and the importance of early diagnosis and appropriate antimicrobial therapy for wound infection following seawater injury. | 2009 | 19279534 |
| effect of increasing nitrobenzene loading rates on the performance of anaerobic migrating blanket reactor and sequential anaerobic migrating blanket reactor/completely stirred tank reactor system. | a laboratory scale anaerobic migrating blanket reactor (ambr) reactor was operated at nitrobenzene (nb) loading rates increasing from 3.33 to 66.67 g nb/m(3)day and at a constant hydraulic retention time (hrt) of 6 days to observe the effects of increasing nb concentrations on chemical oxygen demand (cod), nb removal efficiencies, bicarbonate alkalinity, volatile fatty acid (vfa) accumulation and methane gas percentage. moreover, the effect of an aerobic completely stirred tank reactor (cstr) re ... | 2009 | 19286317 |
| induction of the histidine decarboxylase genes of photobacterium damselae subsp. damselae (formally p. histaminum) at low ph. | to elucidate the detailed mechanism of histamine production by photobacterium damselae subsp. damselae. | 2009 | 19302297 |
| the nonrandom microheterogeneity of 16s rrna genes in vibrio splendidus may reflect adaptation to versatile lifestyles. | 16s rrna molecules in a microbial strain can differ due to nucleotide variation between their genes. this is a typical trait of fast-growing bacteria to cope with different niches. we investigated characteristics of 16s rrna genes in vibrio splendidus strain pb1-10, from the normal flora of atlantic halibut. sequencing of 16s rrna gene clones detected 35 variable positions in a total of 13 different gene copies. more than two-thirds of the substitutions occurred in regions corresponding to helix ... | 2009 | 19341395 |
| vibrios as causal agents of zoonoses. | vibrios are gram-negative rod-shaped bacteria that are widespread in the coastal and estuarine environments. some species, e.g. vibrio anguillarum and v. tapetis, comprise serious pathogens of aquatic vertebrates or invertebrates. other groups, including grimontia (=vibrio) hollisae, photobacterium (=vibrio) damselae subsp. damselae, v. alginolyticus, v. harveyi (=v. carchariae), v. cholerae, v. fluvialis, v. furnissii, v. metschnikovii, v. mimicus, v. parahaemolyticus and v. vulnificus, may cau ... | 2010 | 19342185 |
| photobacterium damselae subsp. damselae, an emerging pathogen in danish rainbow trout, oncorhynchus mykiss (walbaum), mariculture. | a selection of 16 field isolates of photobacterium damselae from marine rainbow trout farms in denmark was subjected to phenotypic and genotypic characterization and pathogenicity to fish. all isolates belonged to the subspecies damselae, being positive for haemolysis, motility and urease. there were considerable differences in haemolytic properties, some isolates presenting a broad zone of haemolysis and others only a narrow zone. pulsed-field gel electrophoresis revealed a high diversity indic ... | 2009 | 19364386 |
| utilization of mucus from the coral acropora palmata by the pathogen serratia marcescens and by environmental and coral commensal bacteria. | in recent years, diseases of corals caused by opportunistic pathogens have become widespread. how opportunistic pathogens establish on coral surfaces, interact with native microbiota, and cause disease is not yet clear. this study compared the utilization of coral mucus by coral-associated commensal bacteria ("photobacterium mandapamensis" and halomonas meridiana) and by opportunistic serratia marcescens pathogens. s. marcescens pdl100 (a pathogen associated with white pox disease of acroporid c ... | 2009 | 19395569 |