| differential potentiation by nystatin of the effect of antibiotics on yeast and mammalian cells. | the effect of the polyene antibiotic nystatin, used in combination with several other antibiotics, on the membranes of candida albicans sc#8169 (yeast) cells and of chinese hamster ovary and 3t3 (mammalian) cells was investigated. it was concluded from determinations of the viability of the yeast cells and from measurements of deoxyribonucleic acid synthesis by the mammalian cells that nystatin acts synergistically with several antibiotics on yeast cells, but not on cho and 3t3 cells. this selec ... | 1975 | 1171651 |
| time-dependent changes in commissural field potentials in the dentate gyrus following lesions of the entorhinal cortex in adult rats. | previous neuroanatomical work has shown that lesions of the entorhinal cortex in adult rats cause the commissural projections to spread from their normally restricted locus in the inner molecular layer approximately 40-50 mum into the outer molecular layer (that is, into the zone deafferented by the lesion). in the present study we measured the effects of the entorhinal lesion on the distribution of short-latency potentials elicited by commissural stimulation in the molecular layer. studies with ... | 1975 | 1175043 |
| endogenous prostaglandin release contributes directly to coronary artery tone. | an in vitro coronary artery preparation of beef heart was found to synthesize and release continuously large amounts of a prostaglandin of the e type. inhibition of prostaglandin synthesis with aspirin, indomethacin, or eicosa-5,8,11,14-tetraynoic acid induced a sustained contraction of the coronary artery, and pretreatment with indomethacin diminished markedly the output of prostaglandin into the bathing medium. it appears that prostaglandin e1, generated from within the vessel wall itself, may ... | 1975 | 1175084 |
| induction of aryl hydrocarbon hydroxylase in rat lung by marijuana smoke. | | 1975 | 1179429 |
| argemone mexicana poisoning in north india. | | 1975 | 1184546 |
| the histochemical activities of some oxidoreductases in the spinal ganglion of the embryonic and the adult rat. | | 1975 | 1188273 |
| the double domain structure of rhodanese. | | 1975 | 1195402 |
| pharmacological investigations on elaeocarpus ganitrus. | | 1975 | 1197422 |
| proceedings: anaesthetics in porphyria: intravenous induction agents. | | 1975 | 1201181 |
| some observations on the possible nutritional significance of vitamin b12-and folate-binding proteins in milk. absorption of [58co]cyanocobalamin by suckling piglets. | 1. a study was made of absorption of [58co]cyanocobalamin in suckling piglets. cyanocobalamin given at birth and at 7 d of age was efficiently absorbed from the intestine and retained within the body, mostly in the liver. a 10 mug test dose was absorbed no less efficiently than 2-5 mug, despite the virtual absence of intrinsic factor in the gut. in piglets given a 10 mug test dose at different ages between 2-5 and 56 d, there was a marked decrease in the efficiency of retention between about 7 a ... | 1975 | 1201270 |
| metabolic studies of [75se]selenocystine and [75se]selenomethionine in the rat. | 1. the long-term fate in rats of an oral dose of [75se]selenocystine was compared with that of an oral dose of [75se]selenomethionine. 2. urinary and faecal radioactivities were measured during the 1st week and whole-body radioactivity was determined for 10 weeks. rats were killed at weekly intervals for 4 weeks and at weeks 6 and 10 for analysis of tissue distribution of 75se. 3. intestinal absorption of [75se]selenocystine was 81% of the administered dose; that of [75se]selenomethionine was 86 ... | 1975 | 1201271 |
| [variety of effects of vitamins on yeast cells in aerobic conditions]. | the effect of thiamine and biotin on the processes of cell division, assimilation of glucose, and accumulation of the biomass and nitrogen in the cells was studied with the candida yeast. the action of the vitamins depended on the source of nitrogen. in some strains, asparagine can substitute for biotin. biotin has different effect on the production of gamma-aminobutyric acid in candida pulcherrima, c. guilliermondii. c. tropicalis k3-10. high concentrations of arginine were found in c. guillie ... | 1975 | 1214604 |
| [electron microscopic study of double infection induced by potato virus x and potato virus y in nicotiana glutinosa]. | | 1975 | 1214639 |
| [changes of the inner ear in experimental stapedectomy]. | | 1975 | 1230953 |
| [effusions in the dog. 1. clinico-diagnostic study with special reference to the cytology of the fluid]. | | 1975 | 1231052 |
| [effect of denervation of the nasal accessory sinuses on th morphology of mucous membrame (experimental study)]. | | 1975 | 1231130 |
| stimulation and inhibition of dna synthesis in rat thymocytes: action of concanavalin a and wheat germ agglutinin. | | 1975 | 1234047 |
| numerical analysis and computerized identification of the yeast genera candida and torulopsis. | numerical analysis of the published standard descriptions of 104 species of candida and 48 species of torulopsis suggested that the number of species should be reduced to 78 and 33 respectively. four examples are noted of closely matched species differing in ability to assimilate nitrate; these species may be combined as nitrate-variable species. although the ability to form pseudomycelium is the only important difference between the genera, only three examples were noted of candida and torulops ... | 1975 | 1236931 |
| chemical modification as a probe of the topography and reactivity of horse-spleen apoferritin. | in apoferritin, but not in ferritin, 1.0 +/- 0.1 cysteine residue per subunit can be modified. in ferritin 3.3 +/- 0.3 lysine residues and 7.1 +/- 0.7 carboxyl groups per subunit can be modified, whilst the corresponding values for apoferritin are 4.4 +/- 0.4 lysine residues and 11.0 +/- 0.4 carboxyl groups per subunit. modification of lysine residues which maleic anhydride and carboxyl groups with glycineamide in apoferritin which has been dissociated and denatured in guanidine hydrochloride le ... | 1976 | 1248472 |
| familial clustering of selective iga deficiency. | serum iga levels of 35 healthy iga-deficient index cases, of their 180 first-degree relatives, and of 31 other family members were studied. iga deficiency was detected in 7.2% of the first-degree relatives, which is significantly more than the 0.25% frequency of iga deficiency in healthy finnish blood donors. subnormal serum iga levels were found in additional 13 (7.2%) first-degree relatives. the familial clustering of iga deficiency seemed to be controlled by multigenic factors. iga-deficient ... | 1976 | 1251582 |
| [diagnostic problems in gout in a 44-year-old man]. | | 1976 | 1251615 |
| the detection of antibodies in human and animal filariases by counterimmunoelectrophoresis with dirofilaria immitis antigens. | counterimmunoelectrophoresis revealed the presence of precipitin antibody in all of 6 dogs and the 1 cat infected with dirofilaria immitis and in the serum of 17 of 24 individuals living in a setting of hyperendemic subperiodic bancroftian filariasis. antigens used in the test were prepared from microfilariae and adult male d. immitis. some humans and animals had antibodies to both antigens while others had antibodies against microfilariae or adult worms only. the presence of soluble circulating ... | 1976 | 1262695 |
| attenuated, streptomycin-dependent salmonella typhi oral vaccine: potential deleterious effects of lyophilization. | four studies were done with streptomycin-dependent salmonella typhi as an oral, attenuated vaccine. studies 1 and 3 employed freshly harvested vaccine, whereas studies 2 and 4 involved lyophilized vaccine. five to eight doses (3 x 10(10)-10(11) organisms/dose) were given; oral streptomycin (1.0 g) was administered concomitantly in studies 2 and 3, with only two of the doses of vaccine in study 1, and was not given in study 4. no adverse reactions were encountered in 179 vaccinated men, and 94% o ... | 1976 | 1262709 |
| measurement and alteration of the capacity of the distended biliary tree in the rat. | | 1976 | 1273853 |
| [changes in the activity of succinate dehydrogenase in the cells of different brain structures under the effect of uhf field of low intensity]. | | 1976 | 1274298 |
| glial cells and extracellular potassium: their relationship in mammalian cortex. | simultaneous recordings were made of glial cell potentials and the extracellular potassium concentration ([k+]o) in cat cortex in an attempt to provide more quantitative information about the sensitivity of mammalian neuroglia to changes in [k+]o. a penicillin epileptogenic focus served to generate both transient and sustained elevations in [k+]o, thus allowing measurement of glial membrane potential (vm) at both resting and increased [k+]o levels many times during the same experiment. resting v ... | 1976 | 1276917 |
| absolute configuration of 3-hydroxy fatty acids present in lipopolysaccharides from various bacterial groups. | the absolute configuration of 3-hydroxy fatty acids has been studied, which are present in the lipopolysaccharides of the following bacteria: phodopseudomonas gelatinosa, rh. viridis, rhodospirillum tenue, chromobacterium violaceum, pseudomonas aeruginosa, bordetella pertussis, vibrio metchnikovii, vibrio cholerae, salmonella spp., escherichia coli, shigella flexneri, proteus mirabilis, yersinia enterocolitica and fusobacterium nucleatum. the 3-hydroxy acids were liberated by strong alkaline hyd ... | 1976 | 1278168 |
| monoclonal antibodies against salmonella porins: generation and characterization. | monoclonal antibodies (mabs) were generated against porins, one of the major outer membrane proteins of salmonella typhi. six clones, designated mp1, mp2, mp3 (igg2ak), mpn4, mpn6 (igg1k) and mpn5 (igg2bk) were characterized by enzyme immunoassay (elisa) for their reactivity to porins from s. typhi, salmonella paratyphi a, s. paratyphi b, s. paratyphi c, salmonella choleraesuis, salmonella enteritidis, salmonella krefeld, salmonella panama, salmonella typhimurium, escherichia coli b, shigella fl ... | 1992 | 1280248 |
| acetylation of o-specific lipopolysaccharides from shigella flexneri 3a and 2a occurs in escherichia coli k-12 carrying cloned s. flexneri 3a and 2a rfb genes. | most of the shigella flexneri o-specific serotypes result from o-acetyl and/or glucosyl groups added to a common o-repeating unit of the lipopolysaccharide (lps) molecule. the genes involved in acetylation and/or glucosylation of s. flexneri lps are physically located on lysogenic bacteriophages, whereas the rfb cluster contains the biosynthesis genes for the common o-repeating unit (d.a.r. simmons and e. romanowska, j. med. microbiol. 23:289-302, 1987). using a cosmid cloning strategy, we have ... | 1992 | 1280255 |
| immunocytochemistry of oculomotor afferents in the squirrel monkey (saimiri sciureus). | attempts were made to co-define afferents of the oculomotor nuclear complex (omc) and their putative neurotransmitters in the squirrel monkey. wheat germ agglutinin conjugated to horseradish peroxidase (wga-hrp) and wheat germ agglutinin conjugated to enzymatically inactive hrp and coupled to colloidal gold (wgaapohrp-au) were used as retrograde tracers in combination with immunocytochemical methods. primarily unilateral injections were made into portions of the omc. stabilized tetramethylbenzid ... | 1992 | 1280294 |
| development of a competitive radioimmunoassay and a sandwich enzyme-linked immunosorbent assay for recombinant human granulocyte colony-stimulating factor. application to a pharmacokinetic study in rats. | a competitive radioimmunoassay (ria) and a sandwich enzyme-linked immunosorbent assay (elisa) for the measurement of recombinant human granulocyte colony-stimulating factor (rhg-csf) in rat serum was developed using polyclonal antibody obtained by immunizing rabbits with rhg-csf. the anti-rhg-csf antibody did not recognize other colony-stimulating factors or interleukins. ria and elisa gave satisfactory reproducibility as judged by intra- and inter-assay precision. good agreements between the ri ... | 1992 | 1280299 |
| differentiation of shigella flexneri strains by rrna gene restriction patterns. | we studied the restriction endonuclease cleavage patterns of rrna genes (ribotypes) of 72 clinical isolates of shigella flexneri representing eight serotypes to determine whether ribotyping could be used to distinguish s. flexneri strains and to compare the discriminating ability of the method with that of serotyping. by using a cloned escherichia coli rrna operon as the probe, southern blot hybridization of restriction endonuclease-digested total dna was carried out. ribotyping of the isolates ... | 1992 | 1280647 |
| regeneration of an adult peripheral nerve preparation in culture. | the methods used to maintain the vagus nerve from the adult rat in culture and how regeneration is studied in this preparation are described. a hypothesis is presented on the triggering of the cell body reaction. it is suggested that this reaction is initiated by proteins synthesized in nonneuronal cells at the site of a nerve lesion. these proteins, referred to as regenerins, reach the nerve cell body by retrograde axonal transport, where they initiate the regeneration process. | 1992 | 1282333 |
| computer-assisted analysis of the structure of regular branched polysaccharides containing 2,3-disubstituted rhamnopyranose and mannopyranose residues on the basis of 13c nmr data. | a computer-assisted approach to the analysis of the structure of branched polysaccharides that contain 2,3-di-o-glycosylated alpha-rhamnopyranose and alpha-mannopyranose residues is based on evaluation of the 13c nmr spectra, using glycosylation effects and their deviations from additivity (delta delta values) at the branch points. this approach, in combination with monosaccharide and methylation analysis data, has been verified on a series of bacterial polysaccharides of known structure. | 1992 | 1284111 |
| multiresistant shigella species from african aids patients: antibacterial resistance patterns and application of the e-test for determination of minimum inhibitory concentration. | the antibacterial resistance pattern and minimum inhibitory concentrations (mic) of 25 shigella flexneri, 5 s. boydii, 8 s. sonnei, and 3 strains of s. dysenteriae type 2 isolated from kenyan prostitutes with bacillary dysentery and aids were determined, and the applicability of the e-test for mic determination evaluated. all strains were resistant to > or = 3 of 9 different antibacterial agents tested. all strains were resistant to tetracycline and erythromycin, 95% to trimethoprim/sulfonamide, ... | 1992 | 1287807 |
| orientation bandwidth: the effect of spatial and temporal frequency. | the orientation bandwidths of psychophysically defined channels of human vision were estimated by two techniques for a wide range of spatial and temporal frequencies. the first technique was an adaptation paradigm, where the subjects' ability to see patterns of various orientations was measured before and after adapting to a high contrast pattern. the second technique evaluated subjects' ability to discriminate between two gratings of different orientations in relation to their ability to detect ... | 1992 | 1287993 |
| [the intoxication syndrome in acute dysentery]. | | 1992 | 1293830 |
| [shigella flexneri strains having a new type antigen 89-141]. | two shigella strains (89-141 and 89-11) isolated from the stool of patients returning from abroad (both from india) in tokyo in 1989 showed an atypical serologic reaction of agglutination with only polyvalent antiserum to s. flexneri prepared commercially. these strains had the typical biochemical characteristics of s. flexneri and were biochemically identical. both strains were positive for serény test in guinea pig eye and cell-invasion test in hela cells. the strains also had virulence-plasmi ... | 1992 | 1294667 |
| [studies of the lymphatic system in mice infected with trichinella pseudospiralis as well as infected and stimulated by phytohaemagglutin (pha-p)]. | mice cfw were infected per os with trichinella pseudospiralis (150 larvae/mice). the mice were divided in two groups: nonstimulated and stimulated intraperitoneally with pha-p at dose 10 mg/kg body weight. the animals were killed 7, 14, 21, 28, 42, 56, 70 and 150 day post infection (d.p.i.). spleen et mesenteric lymph nodes were examined histopathologically (hematoxylin-eosin) and histochemically (nonspecific esterase reaction) for differentiation of t lymphocytes. the obtained results reveal th ... | 1992 | 1299055 |
| effect of outer membrane proteins from shigella on humoral immunity induced in mice by srbc. | shigella flexneri outer membrane proteins (omp) which had been earlier found to exert immunomodulatory effect on cell mediated immune response were also found to act as immunomodulator of the humoral immune response. effects of omp were investigated in the experiments in vitro and in vivo, where the level of humoral immune response, measured as the number of plaque-forming cells (pfc) to srbc in the spleen was evaluated. we demonstrate that small doses of omp (1-5 micrograms) stimulate, whereas ... | 1992 | 1299171 |
| fraction of spleen cells responsible for suppression of cellular immune response to srbc in mice treated with outer membrane proteins of shigella. | effect of splenocytes isolated from mice immunized with suppressive dose of omp from shigella on delayed hypersensitivity, induced in mice with sheep red blood cells was investigated. only the population of t lymphocytes was found to suppress the delayed hypersensitivity, as measured by the footpad reaction. the results suggest that omp of shigella are able to induce in the spleens of animals active t cells which are responsible for the suppression of cellular response induced by srbc. | 1992 | 1299172 |
| [the clinical assessment of macrophage functional activity in patients with protracted dysentery and its correction with lysozyme and vitamin e]. | prospects for the correction of disturbances in the macrophagal system with a combination of lysozyme and vitamin e in patients with a protracted course of dysentery caused by shigella flexneri 1b were studied. the phagocytic activity of macrophages (pam) was found to be suppressed as early as at the beginning of the disease. out of 38 persons repeatedly found to release shigellae 24 were administered polychemotherapy. pam indices in patients treated with lysozyme and tocopherol acetate were lik ... | 1992 | 1301666 |
| expression and release of neuroregulators during development: monoamines and neuropeptides. | the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ... | 1992 | 1306802 |
| hypoxic-ischemic damage in the neonatal brain: excitatory amino acids. | perinatal brain damage is a major clinical problem. recent studies suggest that excitatory amino acids (eaas) may be important for the development of hypoxic-ischemic brain injury in the newborn. experimental work demonstrates that the immature brain is hypersensitive to the toxic effects eaa ('excitotoxicity'), hypoxic-ischemia is accompanied by an extracellular overflow of eaas and hypoxic-ischemic brain damage is reduced by eaa receptor antagonists. clinical investigations demonstrate the pre ... | 1992 | 1306803 |
| [tubo-ovarian transplantation in the treatment of sterility. experimental research]. | microsurgical transposition of fallopian tube and ovary has the potential of being an efficient therapeutic treatment in patients with tubal sterility. the authors present their experience of microsurgical adnexal transplantation in rabbit by two different techniques: the first procedure by microvascular anastomosis of the ovarian vessels, the second one without vascular pedicle. function is evaluated at various time after grafting by: exploratory laparotomy on day 30 to establish whether circul ... | 1992 | 1307024 |
| [the effect of short-term adaptation to high-altitude conditions on human blood serum lipoproteins]. | | 1992 | 1307051 |
| spontaneous insertion of an is1-like element into the virf gene is responsible for avirulence in opaque colonial variants of shigella flexneri 2a. | colonial variation of shigella flexneri serotype 2a from the translucent (2457t) to the opaque form (2457o) occurs spontaneously once in 10(4) cell divisions, with concomitant loss of ipa gene expression and virulence. the appearance of 2457o was associated with the insertional inactivation of virf, an invasion plasmid-encoded positive regulator of ipa gene expression. plasmid pwr110, a tn5-tagged invasion plasmid that restores the invasive phenotype to plasmid-cured shigella derivatives, was co ... | 1992 | 1309511 |
| regulation of interleukin-1 receptors on att-20 mouse pituitary tumour cells. | to study the cellular mechanisms of interleukin-1 (il-1) in the pituitary corticotroph, we studied the properties of il-1 receptors on a mouse pituitary acth-producing cell line, att-20. scatchard plot analysis revealed a single type of receptor with a kd (dissociation constant) of 93 pm, and 482 binding sites/cell. [125i]il-1 alpha binding competed with il-1 alpha and il-1 beta in an equimolar fashion. a 24 h pre-incubation with either crh, epinephrine or nor-epinephrine increased the [125i]il- ... | 1992 | 1312014 |
| regulation of interleukin-1 receptors on att-20 mouse pituitary tumour cells. | to study the cellular mechanisms of interleukin-1 (il-1) in the pituitary corticotroph, we studied the properties of il-1 receptors on a mouse pituitary acth-producing cell line, att-20. scatchard plot analysis revealed a single type of receptor with a kd (dissociation constant) of 93 pm, and 482 binding sites/cell. [125i]il-1 alpha binding competed with il-1 alpha and il-1 beta in an equimolar fashion. a 24 h pre-incubation with either crh, epinephrine or nor-epinephrine increased the [125i]il- ... | 1992 | 1312014 |
| surface presentation of shigella flexneri invasion plasmid antigens requires the products of the spa locus. | an avirulent, invasion plasmid insertion mutant of shigella flexneri 5 (phs1059) was restored to the virulence phenotype by transformation with a partial hindiii library of the wild-type invasion plasmid constructed in pbr322. western immunoblot analysis of phs1059 whole-cell lysates revealed that the synthesis of the invasion plasmid antigens virg, ipaa, ipab, ipac, and ipad was similar to that seen in the corresponding isogenic s. flexneri 5 virulent strain, m90t. ipab and ipac, however, were ... | 1992 | 1312536 |
| correlation between coxsackie b1 virus replication and enhanced invasiveness of shigella flexneri. | coxsackie b1 virus infection enhances the susceptibility of cultured hep-2 cells to shigella flexneri invasiveness. this can be reproduced partially with uv-inactivated virus, particularly the effect observed shortly after viral inoculation. the following phases of viral multiplication were correlated with the enhancing effect: uncoating of viral particles, synthesis of viral rna and proteins, and assembly of newly produced virus particles. uncoating of virus particles was completed within 60 mi ... | 1992 | 1314061 |
| detection of porin antigen in serum for early diagnosis of mouse infections with salmonella typhimurium. | the monoclonal antibodies to porin, an outer membrane protein isolated from salmonella typhimurium and sandwich enzyme linked immunosorbent assay (elisa) has made possible the detection of porin from sera of s. typhimurium-infected mice. the specificity of the monoclonal antibodies was ascertained based on their cross-reactivity with porins isolated from s. typhi, shigella flexneri and escherichia coli and lipopolysaccharide (lps) of s. typhimurium and e. coli. serum samples were found to be pos ... | 1992 | 1315558 |
| use of adeno-associated virus as a general transduction vector for mammalian cells. | | 1992 | 1316261 |
| global regulation of mitochondrial biogenesis in saccharomyces cerevisiae: abf1 and cpf1 play opposite roles in regulating expression of the qcr8 gene, which encodes subunit viii of the mitochondrial ubiquinol-cytochrome c oxidoreductase. | the multifunctional dna-binding proteins abf1 and cpf1 bind in a mutually exclusive manner to the promoter region of the qcr8 gene, which encodes 11-kda subunit viii of the saccharomyces cerevisiae mitochondrial ubiquinol-cytochrome c oxidoreductase (qcr). we investigated the roles that the two factors play in transcriptional regulation of this gene. to this end, the overlapping binding sites for abf1 and cpf1 were mutated and placed in the chromosomal context of the qcr8 promoter. the effects o ... | 1992 | 1317009 |
| kappa-opioid receptor stimulation abolishes mu- but not delta-mediated inhibitory control of spinal met-enkephalin release. | the possible opioid control through delta, mu and kappa receptors of the spinal release of met-enkephalin-like material (melm) was investigated in halothane-anaesthetized rats. the intrathecal perfusion of the delta agonist dtlet (10 microm) or the mu agonist dago (10 microm) resulted in a marked inhibition of melm release, which could be prevented by the selective antagonists naltrindole and naloxone, respectively. although the kappa agonist u 50488 h (10 microm) was inactive per se, it complet ... | 1992 | 1317026 |
| radiation-induced generation of chlorine derivatives in n2o-saturated phosphate buffered saline: toxic effects on escherichia coli cells. | the radiolysis of aqueous chloride solutions has been investigated using pulse and steady-state methods. we have found a correlation between the yields of cl2- and hocl formed in pulse-irradiated n2o-saturated solutions. the yields increased with the increasing concentrations of cl- and phosphate. phosphate enhanced the yield of cl2- in neutral solutions because of a proton transfer from h2po4- to hocl- with a rate constant of (2.6 +/- 0.5) x 10(8) m-1s-1. hocl could not be detected in pulse-irr ... | 1992 | 1317323 |
| ion channels activated by swelling of madin darby canine kidney (mdck) cells. | according to previous studies hyposmotic swelling of madin darby canine kidney (mdck) cells leads to a marked decrease of cell membrane resistance. the present study has been performed to identify the underlying ion channels using the patch-clamp technique: reduction of extracellular osmolarity to 230 mmol/liter leads to a transient activation of k+ channels and a sustained activation of anion channels. the k+ channels are inwardly rectifying with a single-channel slope conductance of 56 +/- 3 p ... | 1992 | 1317457 |
| plasma testosterone concentration in adult male rats following acquisition of copulation-illness associations. | prior research has identified stimuli and procedures that elicit increments in plasma testosterone in copulating male rats. in the present experiment, we demonstrate that associative inhibition of copulatory behaviors in male rats is not correlated with and cannot be attributed to a conditioned suppression of testosterone. each male rat was paired with an inaccessible estrous female for 7 min and was then given an opportunity to copulate. two groups received an injection of either lithium chlori ... | 1992 | 1317591 |
| coxsackie b1 virus-induced changes in cell membrane-associated functions are not responsible for altered sensitivity to bacterial invasiveness. | to analyze the possible mechanisms by which coxsackie b1 virus infection affects the invasiveness of shigella flexneri, we have studied the influence of intracellular levels of na+ and k+, atpase activity, cytoplasmic membrane potential, camp level and cell communication through gap junctions. 3h after adsorption of viable or uv-inactivated coxsackie b1 virus the na(+)-k+ gradient of the cell collapsed, atpase activity decreased, the cytoplasmic membranic potential-dependent tetraphosphonium ion ... | 1992 | 1318709 |
| new monoclonal antibodies for the detection of immediate early antigens of cytomegalovirus. | two new monoclonal antibodies, cie-1 and cie-2, were developed for the rapid detection of human cytomegalovirus (hcmv) infection. they were found to be reactive with immediate early protein of hcmv in the nuclei of infected fibroblasts, as early as 3 hours post-infection. by radioimmunoprecipitation, cie-1 was found to react with a protein with an apparent molecular weight of 70,000, whereas cie-2 precipitated 2 proteins of 70,000 and 72,000 daltons, respectively. both monoclonal antibodies reco ... | 1992 | 1319172 |
| lactoferrin-binding proteins in shigella flexneri. | the ability of shigella flexneri to interact with lactoferrin (lf) was examined with a 125i-labeled protein-binding assay. the percent binding of human lactoferrin (hlf) and bovine lactoferrin (blf) to 45 s. flexneri strains was 19 +/- 3 and 21 +/- 3 (mean +/- standard error of the mean), respectively. 125i-labeled hlf and blf binding to strain m90t reached an equilibrium within 2 h. unlabeled hlf and blf displaced the 125i-hlf-bacteria interaction in a dose-dependent manner. the lf-bacterium co ... | 1992 | 1319403 |
| enrofloxacin treatment of long-tailed macaques with acute bacillary dysentery due to multiresistant shigella flexneri iv. | thirty-four cases of acute bacillary dysentery occurred within 90 days among macaques housed at the california regional primate research center. cases were identified by depression, diarrhea with blood and leukocytic exudate, and/or leukocytosis with a left shift. antimicrobial susceptibility testing of enteric isolates and plasmid profile analyses established an etiologic diagnosis of multiple antibiotic resistant shigella flexneri iv infection. when standard therapies were invalidated by high ... | 1992 | 1320152 |
| serotonergic fibres degenerating in the aging rat brain or sprouting from grafted fetal neurons are not affected by the neurotrophic acth analogue org 2766. | the effects of chronic treatment with the purported neurotrophic factor acth(4-9) analogue org 2766 were studied on age-related degeneration of serotonergic fibres and on gliosis in the rat hippocampus and caudate putamen complex. in addition, the potential growth-promoting effects of org 2766 were investigated on fetal serotonergic cells implanted in a previously denervated hippocampus of young adult rats. chronic treatment of rats from the age of 11 months to 17-18 months did not affect the in ... | 1992 | 1326283 |
| [inhibitory effect of refined amorphophallus konjac on mnng-induced lung cancers in mice]. | 550 seven-wk-old laca mice were used in 3 batches for studying the inhibitory effect of refined amorphophallus konjac (konjaku powder) on mnng-induced lung cancers. the mice (within each batch) were randomly allocated into four groups, namely, positive control (mnng), amorphophallus konjac (a. k.), complex (mnng+a. k.), and blank control (c) groups. in mnng group, mnng (250 micrograms) was injected intravenously once every five days for seven times in each mouse, the total dosage of mnng being 1 ... | 1992 | 1327690 |
| interferon treatment reduced adherence, invasiveness and intracellular multiplication of shigella flexneri in coxsackie b1 virus-infected cells. | the effect of interferon treatment on interaction of shigella flexneri with in vitro cultured cells was investigated. pretreatment of hep-2 cells with human interferons had no effect on the susceptibility of cells to s. flexneri, measured by invasiveness and adhesiveness. human leukocyte interferon and human recombinant interferon-alpha-a reduced adhesiveness, intracellular multiplication and invasiveness of s. flexneri in hep-2 cells preinfected with coxsackie b1 virus. also non-receptor mediat ... | 1992 | 1329437 |
| regulation of virulence-associated plasmid genes in enteroinvasive escherichia coli. | the transposon tnphoa was used for construction of gene fusions and for studies of gene regulation in an enteroinvasive strain of escherichia coli. several plasmid-encoded virulence genes (e.g., the ipab and virg operons) of such enteroinvasive strains are subject to coordinated thermoregulation involving both operon-specific (the virb and virf activators) and global regulators. the nucleoid-associated e. coli protein h-ns was shown to be a negative regulator as judged by studies using h-ns gene ... | 1992 | 1332938 |
| mxij, a lipoprotein involved in secretion of shigella ipa invasins, is homologous to yscj, a secretion factor of the yersinia yop proteins. | shigella flexneri causes bacillary dysentery by invading epithelial cells of the colonic mucosa. the invasion process requires the synthesis and secretion of the virulence plasmid-encoded ipa proteins. using tnphoa mutagenesis, we have identified two virulence plasmid genes, mxij and mxim, that encode proteins exported by the general export pathway. analysis of the mxij and mxim deduced amino acid sequences suggested that mxij and mxim might encode lipoproteins, which was confirmed by [3h]palmit ... | 1992 | 1332940 |
| [toxic megacolon due to shigella: a report of a new case]. | a new case of shigella-caused megacolon is described in detailed form. some considerations are made about frequency, pathology, clinic and prognostic features. | 1992 | 1340679 |
| [shigellosis in children of the ix region of chile: clinical and epidemiologic aspects and antibiotic sensitivity]. | ninety four children with diarrhea and a positive stool culture for shigella, hospitalized at the hospital regional de temuco, were studied. forty six percent of patients were less than two years old. forty two percent of microorganisms were resistant to ampicillin, 45% to trimethoprim/sulfamethoxazole, 8% to tetracycline and none to chloramphenicol. isolated species were shigella flexneri 83% and shigella sonnei 17%. seventy nine percent of patients had fever, 60% dysentery and 21.3% seizures. ... | 1992 | 1340946 |
| [bacteriology of shigella isolated in a tropical zone chu]. | from 1981 to 1991, five hundred eighty one shigella's strains were isolated in the laboratories of the chu fann of dakar. they represent 24.8 per cent of enteropathogen agents and 4.3 per cent of diarrhoeae causative agents. shigella flexneri was predominant (71%), following by shigella dysenteriae (14.5%), shigella sonnei (11.4%) and shigella boydii (2.5%). in pediatric hospital 90 per cent of strains were isolated between 1 and 5 years age; in adults, the pic was found from 20 to 30 years. fiv ... | 1992 | 1345084 |
| effect of overproduction of heat shock chaperones groesl and dnak on human procollagenase production in escherichia coli. | the effect of overexpression of the heat shock chaperone genes dnak and groesl on heterologous protein production in escherichia coli was examined, using a set of related human procollagenase proteins. a diverse range of effects on protein solubility, secretion, and accumulation was observed, and these effects were highly dependent on the particular chaperone/procollagenase pairing involved. both chaperones caused a large increase in the apparent solubility of a fusion of the lamb signal peptide ... | 1992 | 1346610 |
| brain somatostatin receptor-g protein interaction. g alpha c-terminal antibodies demonstrate coupling of the soluble receptor with gi(1-3) but not with go. | somatostatin (sst) receptors activate potassium channels, stimulate protein phosphatases, inhibit adenylate cyclase and close calcium channels. these multiple effects are controlled by guanine nucleotide binding (g) proteins of the pertussis toxin-sensitive gi and go types. in the present study we have identified the g proteins coupling with brain sst receptors. to this end, brain sst receptors were solubilized in g-protein coupled form. binding of the sst analogue mk 678 to the solubilized rece ... | 1992 | 1346612 |
| calcium utilization coupled to stimulation of postjunctional alpha-1 and alpha-2 adrenoceptors in isolated human resistance arteries. | human s.c. resistance arteries (internal diameters 158-353 microns) were mounted in a microvascular myograph, and experiments were designed to examine the calcium pools utilized by selective stimulation of alpha-1 and alpha-2 adrenoceptors. in a concentration-dependent manner, phenylephrine and b-ht 933 evoked contractions mediated by alpha-1 and alpha-2 adrenoceptors, respectively, both in calcium-containing and in calcium-free saline. with respect to the maximum response to potassium in calciu ... | 1992 | 1346639 |
| sexual transmission of htlv infections in southern india. | | 1992 | 1346809 |
| medial vestibular nucleus in the guinea-pig: nmda-induced oscillations. | we have recently shown in vivo that n-methyl-d-aspartate (nmda) receptors are present in the guinea-pig vestibular complex and demonstrated that they are involved in the regulation of the resting discharge of vestibular neurones. a parallel in vitro study has identified in the guinea-pig medial vestibular nuclei (mvn) two main neuronal cell types, a and b mvnn, differing by their intrinsic membrane properties. one subtype of b mvnn was further characterized by the presence of a low threshold cal ... | 1992 | 1347271 |
| purification and determination of glutamine synthetase by high-performance immunoaffinity chromatography. | high-performance immunoaffinity chromatography (hpiac) with anti-glutamine synthetase polyclonal antibodies bound to epoxy-activated silica was used to purify and determine this enzyme from the cyanobacterium synechocystis. a single-step hpiac procedure with cell-free extracts yielded electroporetically homogeneous glutamine synthetase. in the determination of glutamine synthetase by hpiac a linear response in the range 10-60 micrograms of enzyme was observed. recoveries of 70% of the loaded enz ... | 1992 | 1347294 |
| phenotypic and functional studies on ocular t cells during herpetic infections of the eye. | herpetic stromal keratitis an inflammatory disease of the eye resulting from herpes simplex virus type 1 infection, is a common cause of blindness. the disease is generally considered to represent an immunopathologic response, but the exact mechanism remains in doubt and is subject to debate. we have investigated the nature of inflammatory cells in the eye and have isolated ocular cells to establish their phenotype and determine some of their functions. by means of immunocytochemistry and cytofl ... | 1992 | 1347309 |
| [h2-receptor antagonist-refractory ulcer--its pathophysiology and role of proton-pump inhibitors]. | among h2-receptor antagonist (h2ra)-refractory ulcers, non-responders that did not heal after 5 months therapy had high intraluminal ph in the basal condition and high sensitivity to inhibition of acid secretion by h2ra but possessed gastric mucosa to generate less prostaglandins. combination therapy of pge1-analogue with h2ra healed these ulcers by 60%. proton-pump inhibitor (ppi) exerted a complete inhibition of acid secretion in these patients and the rate of healing was 88%. helicobacter pyl ... | 1992 | 1347325 |
| testing an anti-hiv trio. | | 1992 | 1347353 |
| modification of dena-induced hepatocarcinogenesis by ccl4 cirrhosis. comparison of the marker enzyme patterns. | the modifying action of chronic liver injury on the process of hepatocarcinogenesis was investigated. to induce cirrhosis or fibrosis f344 rats received ccl4 alone or in combination with phenobarbital, either before (model 1) or after (model 2) the application of initiator, diethylnitrosamine (dena). in these models, morphology, tumor incidence as well as polysubstrate monooxygenase system, gamma-glutamyltransferase (ggt) and glucose-6-phosphatase (g-6-pase) were studied. the data presented show ... | 1992 | 1350234 |
| a model of interaction between entamoeba histolytica and shigella flexneri. | the establishment of a relationship between entamoeba histolytica and certain bacteria may contribute to the expression and/or enhancement of the pathogenicity of this parasite. recent experiments have shown that bacteria expressing mannose-binding lectins on their surface could attach to mannose-containing molecules on the surface of amoebae. in this study, we established a model of interaction between. e. histolytica and shigella flexneri. using well-characterized mutants of s. flexneri, we st ... | 1992 | 1353632 |
| characterization of serpulina (treponema) hyodysenteriae and related intestinal spirochetes by ribosomal rna gene restriction patterns. | serpulina (treponema) hyodysenteriae strain a-1 partially purified rrna, labelled with photobiotin, was used as a non-radioactive probe to identify the rrna gene restriction patterns of s. hyodysenteriae strains and other spirochetes. sau3a restriction enzyme digests resulted in similar rrna gene restriction patterns in s. hyodysenteriae strains from five different countries. some s. hyodysenteriae strains could be differentiated by variations in their rrna gene restriction patterns after cleava ... | 1992 | 1354196 |
| glutathione homeostasis in rats chronically treated with ethanol. evidence for an increased hepatic gsh export in vivo. | the influence of chronic ethanol feeding to rats on the hepatic glutathione (gsh and gssg) system (synthesis, catabolism, export) and on the gsh and gssg concentrations in extrahepatic tissues was investigated. histological examination of livers from ethanol pretreated rats revealed a minor dilatation of the hepatic sinusoids. after ethanol administration the distribution pattern of gamma-glutamyltranspeptidase (enzymehistochemistry) was nearly unchanged, but the hepatic activity of this enzyme ... | 1992 | 1360286 |
| hepatitis b virus infection among aboriginal children in eastern taiwan. | hepatitis b virus (hbv) infection is endemic in taiwan, with 15-20% of the adult population carrying the surface antigen (hbsag). hualien in eastern taiwan is relatively isolated from other parts of the island by the central mountains. the status of hbv infection among mountain aborigines in hualien has not been studied on a large scale before. a survey of serum hbsag and its antibody (anti-hbs) was conducted in 1989. a total of 3,287 children from 27 primary schools in three mountainous countie ... | 1992 | 1360296 |
| inhibitory effect of a microcystis sp (cyanobacteria) toxin on development of preimplantation mouse embryos. | 1. a soluble toxin, purified from the algae bloom of an eutrophic lake dominated by microcystis, is a very effective inhibitor of early embryo development in a dose-response relationship. 2. two- and 8-cell mouse embryos under the influence of microcystis toxin do not reach the developmental stages of morula and blastocyst, respectively. 3. actin cortex is disorganized without change in the microtubules structure. 4. results are discussed in terms of the possible mechanisms by which the toxin ar ... | 1992 | 1360361 |
| transport characteristics of cephalosporin antibiotics across intestinal brush-border membrane in man, rat and rabbit. | the uptake of orally active cephalosporins, ceftibuten and cephradine, by intestinal brush-border membrane vesicles isolated from man, rat and rabbit was studied. in the presence of an inward h+ gradient, ceftibuten but not cephradine was taken up into intestinal brush-border membrane vesicles of man and rat against the concentration gradient (overshoot phenomenon). in rabbit jejunal brush-border membrane vesicles, the uptake of both cephalosporins in the presence of an inward h+ gradient exhibi ... | 1992 | 1361560 |
| studies on novel pili from shigella flexneri. i. detection of pili and hemagglutination activity. | pili were detected using electron microscopy in clinical isolates of shigella flexneri which had been continuously subcultivated in liquid media. morphologically, the pili appeared as thin, flexible, cylindrical structures of up to 2-5 microns in length and about 3-5 nm in diameter. two strains showed mannose-resistant (mr) hemagglutination to fresh fowl erythrocytes (type 4), and one to tannic acid-treated horse erythrocyte (type 3) pili. these pili are novel and different from the mannose-sens ... | 1992 | 1361958 |
| effect of enterovirus infection on susceptibility of hela cells to shigella flexneri invasivity. | invasiveness of shigella flexneri m90t in hela cells was significantly increased when cells were preinfected with poliovirus 1, coxsackievirus b3 and echovirus 6. this effect was dependent on the dose of virus used, evident at early stages of viral infection and lasted hours before the appearance of a cytopathic effect. an increase of bacterial invasion ability was also noticed when hela cells were incubated with uv-inactivated enteroviruses. this enhancing effect obtained with both viable and u ... | 1992 | 1364020 |
| cloning and expression in escherichia coli of mercuric ion resistance coding genes from zymomonas mobilis. | from a genomic library of zymomonas mobilis prepared in escherichia coli, two clones (carrying pzh4 and pzh5) resistant to the mercuric ion were isolated. on partial restriction analysis these two clones appeared to have the same 2.9 kb insert. mercuric reductase activity was assayed from the escherichia coli clone carrying pzh5 and it was hg(2+)-inducible, nadh dependent and also required 2-mercaptoethanol for its activity. the plasmid pzh5 encoded three polypeptides, mercuric reductase (mera; ... | 1991 | 1367242 |
| characterization of zymomonas mobilis alkaline phosphatase activity in escherichia coli. | zymomonas mobilis phoa gene encoding alkaline phosphatase was expressed in escherichia coli cc118 carrying the recombinant plasmid pzap1. the ph optimum for this enzyme was 9.0 and showed a peak activity at 42 degrees c. this enzyme required zn2+ for its catalytic activity; however, mg2+ or ca2+ significantly affected the activity. this enzyme was found to be ethanolabile, and ethanol inhibition was reversed by addition of zn2+. kinetics of z. mobilis alkaline phosphatase production in e. coli c ... | 1992 | 1369189 |
| cloning vectors derived from streptomyces niveus plasmid psn2. | two high copy number, broad host range, general purpose cloning vectors, plg5 and plg10, derived from the unstable streptomyces niveus plasmid psn2 are described. plg5 (5.5 kb) and plg10 (6.5 kb) both carry the thiostrepton resistance (tsrr) and lethal zygosis (ltz+) markers and have single cloning sites within a non-essential region and the tsr gene. plg505 (7.4 kb) was constructed by cloning the viomycin resistance (vph) gene into the single bamhi site of plg5 to give a further vector with ins ... | 1990 | 1369288 |
| myosin-cross-reactive epitope of shigella flexneri invasion plasmid antigen b. | ipab, invasion plasmid antigen b, of shigella flexneri is a 62-kda protein required for invasion of intestinal epithelial cells. ipab is also one of several major protein antigens recognized by the humoral immune systems of most humans and monkeys after infection with shigellae. computer analysis of the deduced ipab amino acid sequence indicates that an alpha-helical structure is likely through much of the molecule. homology searches with protein data banks show that one alpha-helical domain bet ... | 1992 | 1370431 |
| biochemical and functional characterization of the leucocyte tyrosine phosphatase cd45 (cd45ro, 180 kd) from human neutrophils. in vivo upregulation of cd45ro plasma membrane expression on patients undergoing haemodialysis. | the biochemical and functional characterization, and the regulation of plasma membrane expression of the leucocyte tyrosine phosphatase cd45, have been investigated in neutrophils from healthy donors and patients undergoing haemodialysis. cd45 proteins of 180 kd and 130-150 kd were precipitated from neutrophils from both healthy subjects and haemodialysed patients. prolonged storing, as well as trypsin treatment of samples containing the 180-kd cd45 protein, generated the 130-150-kd polypeptides ... | 1992 | 1370931 |
| identification and characterization of b-cell epitopes of ipac, an invasion-associated protein of shigella flexneri. | invasion plasmid antigen c (ipac) is a 43-kda plasmid-encoded protein associated with the ability of shigellae to invade epithelial cells. this protein is consistently strongly recognized by sera from convalescent patients and monkeys experimentally infected with shigellae. the strong immunogenicity of ipac in the course of natural infection makes it a good candidate as a potentially protective antigen. to map the b-cell epitopes of this protein, the gene encoding ipac was cloned and expressed a ... | 1992 | 1373401 |
| immunochemical characterization of citrobacter strain pcm 1487 o-specific polysaccharide- and core oligosaccharide-protein conjugates. | o-specific-polysaccharide and core oligosaccharide were isolated from citrobacter strain pcm 1487 lipopolysaccharide and purified. the polysaccharide was selectively devoid of 4-deoxy-d-arabinohexose residues. covalent conjugates of the modified o-specific polysaccharide and of the core oligosaccharide with tetanus toxoid were prepared. immunochemical characterization of these conjugates proved that they are strong immunogens. using monospecific rabbit antisera raised against the conjugates, the ... | 1992 | 1376131 |
| outward currents in longitudinal colonic muscle cells contribute to spiking electrical behavior. | electrical events in longitudinal and circular muscles of the colon are different. longitudinal muscles generate action potentials superimposed upon small depolarizations termed myenteric potential oscillations and circular muscles generate slow wave events that persist for several seconds. differences between circular and longitudinal muscles may be related to the potassium channels these cells express. we have studied ca(2+)-dependent and voltage-dependent k currents of isolated longitudinal c ... | 1992 | 1378997 |
| the vascular endothelial growth factor proteins: identification of biologically relevant regions by neutralizing monoclonal antibodies. | angiogenesis plays critical roles in organ development during embryonic and fetal life, wound healing and in a variety of pathological conditions. vascular endothelial growth factor (vegf) is a secreted growth factor specific for vascular endothelial cells which induces angiogenesis in vivo. to gain a better understanding of the physiological role of vegf, we have generated and characterized four murine monoclonal antibodies (mabs) using the 165 amino acid species of recombinant human vegf as im ... | 1992 | 1380254 |
| induction of a humoral immune response to a shiga toxin b subunit epitope expressed as a chimeric lamb protein in a shigella flexneri live vaccine strain. | shigella flexneri vaccine strain (sfl124) given orally, evokes humoral immune response in human volunteers. such a strain, expressing antigenic epitope of b subunit of shiga toxin, would also provide immunity to the toxin produced by some species of shigella. a synthetic oligonucleotide, specifying an epitope [13-26 amino acids (aa)] of the b subunit of shiga toxin, was inserted into the lamb gene of escherichia coli and expressed in the s. flexneri vaccine strain. the chimeric lamb protein func ... | 1992 | 1381804 |
| a novel temporal expression pattern of three c/ebp family members in differentiating myelomonocytic cells. | members of the ccaat/enhancer binding protein (c/ebp) family have been shown to regulate the terminal differentiation of adipocytes and hepatocytes. in these cell lineages, high levels of c/ebp alpha are found only in mature, nondividing cells. using western blotting and immunohistochemical staining, we have determined the temporal order of expression for c/ebp alpha, c/ebp beta, and c/ebp delta in differentiating myelomonocytic marrow cells. these studies show a unique temporal pattern of c/ebp ... | 1992 | 1391942 |
| monitoring of antibiotic resistance in shigellae isolated in the netherlands 1984-1989. | during surveillance of antimicrobial resistance in shigella strains isolated in the netherlands from 1984 to 1989 and forwarded to the national institute of public health and environmental protection for typing, sensitivity to twelve antimicrobial agents was assessed. high rates of resistance to the older drugs of choice in treating shigellosis were found, i.e. ampicillin and trimethoprim-sulfamethoxazole. ampicillin resistance varied from 33 to 53% among shigella flexneri strains and from 10 to ... | 1992 | 1396730 |