| [prevention of ischemic and re-oxygenation arrhythmias and heart fibrillation using the antioxidant ionol]. | the effect of a synthetic antioxidant, ionol (2,4-ditrebutyl-4-methylphenol) on cardiac arrhythmias induced by 10-minute occlusion of the left coronary artery followed by 5 minutes of reperfusion (rp) was investigated. the study was performed on male wistar rats, 250-300 g body weight. the animals were ventilated with room air under urethan anesthesia. rp induced more severe ventricular arrhythmias than ischemia (is). during rp ventricular fibrillation developed in 12 and during is in 2 out of 2 ... | 1986 | 3730544 |
| [prevention of ischemic and re-oxygenation arrhythmias and heart fibrillation using the antioxidant ionol]. | the effect of a synthetic antioxidant, ionol (2,4-ditrebutyl-4-methylphenol) on cardiac arrhythmias induced by 10-minute occlusion of the left coronary artery followed by 5 minutes of reperfusion (rp) was investigated. the study was performed on male wistar rats, 250-300 g body weight. the animals were ventilated with room air under urethan anesthesia. rp induced more severe ventricular arrhythmias than ischemia (is). during rp ventricular fibrillation developed in 12 and during is in 2 out of 2 ... | 1986 | 3730544 |
| structure and x-ray amino acid sequence of a bacteriochlorophyll a protein from prosthecochloris aestuarii refined at 1.9 a resolution. | the structure of the water-soluble bacteriochlorophyll a protein (bchl protein) from the green photosynthetic bacterium prosthecochloris aestuarii has been refined at 1.9 a resolution to a crystallographic residual of 18.9%. the refinement was carried out without knowledge of the amino acid sequence and has led to an "x-ray sequence". the structure consists of seven bchl molecules enclosed within a protein "bag" and the refinement supports the general conformation of the molecule described previ ... | 1986 | 3735428 |
| synaptic and golgi membrane recycling in cochlear hair cells. | membrane recycling in the mechanoreceptive sensory cells of the mammalian cochlea was studied by observing membrane-bound horseradish peroxidase (hrp) reaction product following brief in vivo exposure to the enzyme. in the inner hair cell (ihc), peroxidase was taken up into coated vesicles and became incorporated into synaptic vesicles surrounding presynaptic bodies, but much hrp was also transported to the apical zone where reaction product appeared in all components of the golgi complex. neith ... | 1986 | 3746347 |
| [radiation biology risk of imaging procedures]. | the environmental radiation burden of man in germany is about 1 mgy (milligray) per year. this is, of course, also valid for children. due to diagnostical procedures this burden is increased to about 1.3 mgy. the question arises wether this can be neglected, or important consequences have to be drawn. to give a clear answer, the action of ionizing radiation in living cells and in organisms is explained in detail. many of the radiation actions at the dna can soon be repaired by the cell, if the r ... | 1986 | 3748028 |
| [systemic mechanisms of development and compensation of acute hypoxemia in experimental methemoglobinemia in dogs]. | | 1986 | 3748626 |
| 1,25-dihydroxyvitamin d3 treatment results in increased choline acetyltransferase activity in specific brain nuclei. | to better understand the role of 1,25-dihydroxyvitamin d3 [1,25-(oh)2d3] in brain function, the level of calcium-binding protein (cabp) and the activities of choline acetyltransferase (cat) and monoamine oxidase were measured in discrete brain nuclei of vitamin d-deficient and -replete male rats. the nuclei sampled were those in which receptors for 1,25-(oh)2d3 and/or vitamin d-dependent cabp have been localized. significant elevations in cat activity were found in the arcuatemedian eminence and ... | 1986 | 3753932 |
| effect of shigella toxin on preimplantation mouse embryos in vitro. | preimplantation mouse embryos at the 4-cell to 8-cell stage were exposed to shigella dysenteriae toxin at concentrations of 0.001-100 pg/ml in vitro. the effect of the toxin was studied by morphological observation of the embryos to the blastocyst stage, by assessing protein synthesis with 14c-leucine incorporation, and by measuring embryonic adenosine triphosphate (atp) content. preimplantation mouse embryos were highly sensitive to the toxin. all variables investigated were adversely influence ... | 1986 | 3755556 |
| prevalence of toxoplasma antibodies in southern louisiana swine. | sera from 1,219 pigs slaughtered in acadian louisiana in 1980 and 1981 were tested using the indirect hemagglutination test and microtiter system for toxoplasma antibodies and 19.2% had titers of 1:64 or higher. pigs slaughtered in the colder months, november to march, had significantly higher seroprevalences than pigs slaughtered in the warmer months. 24.6% of 236 farm bled sows and gilts had similarly high titers. | 1986 | 3759353 |
| psychoanalysis and time. | psychoanalysis is fundamentally related to time because it is an effort to understand how disturbances in the present are determined by events in the past. technically, we know that the patient who is reporting immediate perceptions is not aware of the passage of time, but he becomes self-conscious as undesirable elements threaten to appear in his associations. time is not sensed by direct awareness, nor is it an agent of action or events. various functions of the ego influence how time is exper ... | 1986 | 3760442 |
| genetic variations in blood proteins within and between spanish dairy sheep breeds. | eleven flocks of three spanish sheep breeds have been studied: four churra, four lacha and three manchega. genetic variations between flocks of the same and different breeds have been analysed, using eight genetic blood systems and a wide range of statistical methods. the results show that the churra, lacha and manchega breeds present significant differences between each other. significant intraracial variations have been observed in churras and lachas, although they were almost non-existent in ... | 1986 | 3767081 |
| effects of subchronic pyridostigmine pretreatment on the toxicity of soman. | the effect of subchronic pyridostigmine pretreatment on the toxicity of soman, in the absence of supporting therapy (atropine, oxime, and (or) anticonvulsant), as well as its effect on muscarinic cholinoceptor binding characteristics was assessed in the rat. pretreatment with pyridostigmine by means of an implanted alzet osmotic minipump for a 5-day total exposure dose of 12 mg/kg inhibited whole blood acetylcholinesterase activity by 73%. this pyridostigmine pretreatment lowered the soman ld50 ... | 1986 | 3768795 |
| [establishment of a spontaneously transformed human fetal lung cell line and its biological characteristics]. | a spontaneously transformed human fetal lung cell line (hflt) was derived from a human fetal lung diploid fibroblast cell line (2bs) by continuous culture. the biological characteristics of this cell line were studied and compared with those of 2bs cells. accompanying the morphological alteration, the growth rate of the transformed cell was accelerated and the maximum cell density was increased. the anchorage-independent growth was shown by its ability to form colonies in soft agar. the collagen ... | 1986 | 3769747 |
| in vivo stability of in vitro labelled 99tcm-red blood cells for radionuclide determination of left ventricular ejection fractions and volumes. | serial determination of radionuclide left ventricular ejection fraction and volumes require a label that remains highly stable after introduction into the human circulation. we have evaluated the in vivo stability of 99tcm-red blood cells (99tcm-rbc) labelled in vitro by preparation with small amounts of a stannous agent in 19 patients with coronary artery disease. the distribution volume of 99tcm-rbc was virtually identical to that of rbc labelled with 51cr. labelling efficiency expressed as th ... | 1986 | 3774253 |
| endosulfan induced inhibition of 3h-5-hydroxytryptamine uptake in platelets. | endosulfan, a chlorinated pesticide, is widely used to control various insect pests. rats exposed to 1 mg and 3 mg endosulfan/kg for periods of 10, 30, and 60 days showed significant (p less than 0.05) inhibition of [3h]5-hydroxytryptamine (5-ht) uptake by platelet-rich plasma (prp) in an ex vivo study. rats treated with endosulfan (1 and 3 mg/kg) up to 60 days elucidated a marked inhibition of adp-induced aggregatory responses of the platelets. incubation of prp with 10 microm and 100 microm en ... | 1986 | 3775804 |
| myoelectric correlates of colonic motor complexes and contractile activity. | | 1986 | 3777176 |
| changes in regional myocardial perfusion by muscarinic receptor subtypes in dogs. | to determine the muscarinic receptor subtype (m1 or m2 or both) responsible for cholinergic coronary vasodilatation and alterations in the transmural distribution (endocardial to epicardial blood flow ratio) of coronary blood flow, systemic and coronary haemodynamic indices and regional myocardial blood flow (radioactive microspheres) were measured in anaesthetised dogs. submaximal vasodilative doses of the mixed muscarinic agonist acetylcholine were given by intracoronary infusion to avoid peri ... | 1986 | 3779744 |
| estimation of regional gas and tissue volumes of the lung in supine man using computed tomography. | this study was intended to discover how well computed tomography could recover the volume and weight of lung like foams in a body like shell, and then how well it could recover the volume and weight of the lungs in supine man. model thoraces were made with various loaves of bread submerged in water. computed tomography scans recovered the volume of the model lungs (true volume range 250-12,500 ml) within +0.2 (sd 68) ml and their weights (true range 72-3125 g) within +30 (78) g. scans also recov ... | 1986 | 3787544 |
| 1h-nmr assignments and the dynamics of interconversion of the isomeric forms of cytochrome b5 in solution. | cytochrome b5 reconstituted with specifically deuterated hemins has led to the assignment of the resolved 6,7 beta-propionate protons and heme meso protons. freshly reconstituted cytochrome b5 contains a mixture of two isomers in an approx. 1:1 ratio. as time proceeds the minor isomer decreases in intensity until the equilibrium ratio, approx. 8:1, of the two isomers is reached. the rate of the heme disorder kinetics was investigated for cytochrome b5 as a function of ph, oxidation state and 2,4 ... | 1986 | 3790573 |
| 1h-nmr assignments and the dynamics of interconversion of the isomeric forms of cytochrome b5 in solution. | cytochrome b5 reconstituted with specifically deuterated hemins has led to the assignment of the resolved 6,7 beta-propionate protons and heme meso protons. freshly reconstituted cytochrome b5 contains a mixture of two isomers in an approx. 1:1 ratio. as time proceeds the minor isomer decreases in intensity until the equilibrium ratio, approx. 8:1, of the two isomers is reached. the rate of the heme disorder kinetics was investigated for cytochrome b5 as a function of ph, oxidation state and 2,4 ... | 1986 | 3790573 |
| glucose regulation of cephamycin biosynthesis in streptomyces lactamdurans is exerted on the formation of alpha-aminoadipyl-cysteinyl-valine and deacetoxycephalosporin c synthase. | glucose exerted a concentration-dependent negative regulation on the biosynthesis of cephamycin c by streptomyces lactamdurans. formation of the cephamycin precursor delta(alpha-aminoadipyl)-cysteinyl-valine was greatly decreased by excess glucose. the ring-expanding enzyme deacetoxycephalosporin c synthase was strongly repressed by glucose in vivo. isopenicillin n synthase (cyclase) and isopenicillin n epimerase were not repressed by glucose. however, the activity of isopenicillin n synthase wa ... | 1986 | 3794640 |
| suppression of free fatty acids and triglycerides in normal and hypertriglyceridemic rats by the adenosine receptor agonist phenylisopropyladenosine. | activation of adenosine receptors leads to the inhibition of lipolysis in the fat cells of several species including humans. we have examined the effects of the adenosine analog phenylisopropyladenosine (pia) on lipolysis in vivo in intact rats. pia suppresses markedly serum-free fatty acid (ffa) concentrations when injected s.c. (-)-pia is more potent than (+)-pia in suppressing ffa concentrations; also, this effect of (-)-pia is blocked by the adenosine receptor antagonist 8-(p-sulfophenyl)the ... | 1986 | 3795036 |
| effect of clofibrate feeding on some lipogenic enzyme activities induced by high carbohydrate diet. | clofibrate administration by stomach tube or intraperitoneally for 3 successive days to rats fed standard diet or starved for 72 hr caused about 2-fold increase of malic enzyme activity in the liver and adipose tissue. the drug administered by stomach tube (but not intraperitoneally) to the rats fed fat free-high carbohydrate diet significantly blocked the inducing effect of the diet on malic enzyme activity in both tissues. clofibrate blocked the induction by fat free-high carbohydrate diet of ... | 1986 | 3801034 |
| pneumonia--the quality of medical records data. | the quality of medical records data for patients who were hospitalized with community-acquired pneumonia was assessed by comparing medical records data with data obtained in a prospective study of pneumonia for the period april 1, 1984, to december 31, 1984. one hundred five patients fulfilled the case definition of pneumonia for entry into the prospective study. one hundred twenty-seven patients were identified by medical records data. seventy-three of the patients appeared in both studies. the ... | 1987 | 3807447 |
| [effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. | | 1985 | 3835674 |
| 4-hydroxyalk-2-enals are substrates for glutathione transferase. | the 4-hydroxyalk-2-enals are established products of lipid peroxidation that are conjugated with intracellular glutathione. cytosolic glutathione transferases from rat liver were shown to give high specific activities with 4-hydroxynonenal and 4-hydroxydecenal. the isoenzyme giving the highest specific activity was glutathione transferase 4-4. the rate of the spontaneous conjugation reaction is negligible in comparison with the rate calculated for the cellular concentration of the glutathione tr ... | 1985 | 3838159 |
| shigella toxin and the pathogenesis of shigellosis. | shigella dysenteriae 1 produces a periplasmic protein with multiple toxic effects in vivo and in vitro. these include neurotoxicity, cytotoxicity and enterotoxicity, as well as the ability to inhibit cell-free protein synthesis. the purified toxin is a protein of relative molecular mass (mr) 64 000. it is composed of one catalytically active a subunit (mr = 32 000) that inhibits protein synthesis, and a complex of five b monomers (mr approximately 6500 each). studies using subunit-specific antib ... | 1985 | 3847336 |
| theoretical mechanisms for synthesis of carcinogen-induced embryonic proteins: xiv. mutational and non-mutational mechanisms as subsets of a more general mechanism. part c. a defined cancer mutation. | the results of applying a mechanism of ethionine-induced embryonic gene derepressions to explain similar features found in hereditary tyrosinemia have been extended to a well defined cancer mutation. in all three cases, the described mechanism is compatible with the explanation for the etiology of embryonic like phenotypic expressions in cells and potentially for the carcinogenic process. the essence of the formulated process for a human bladder carcinoma mutation in the ras gene for a protein p ... | 1985 | 3853079 |
| protein loss of bovine dental enamel during in-vitro subsurface demineralization. | a chemical system based on the dialysis principle was used to study protein loss of dental enamel during demineralization with an acetic-acid buffer solution containing calcium and phosphate, in which the fluoride-ion activity was kept constant. this resulted in a subsurface lesion, with a depth of about 130 microns. after demineralization, protein material was isolated from the demineralization solution. u.v. spectra of the protein showed strong absorbance between 240 and 300 nm. amino-acid com ... | 1985 | 3865639 |
| [the development of the human dentition. iv]. | | 1985 | 3867519 |
| [the development of the human dentition. iv]. | | 1985 | 3867519 |
| [different clinical and prognostic aspects of angina pectoris in unstable phase]. | the purpose of this study was to focus on the clinical and angiographic characteristics of 113 patients with crescendo angina (group i) as compared to 187 patients with angina of new onset (group ii), selected from a series of 474 consecutive subjects, admitted to our clinic between january 1976 and july 1983 because of recurrent episodes of spontaneous angina, who underwent cardiac catheterization and coronary angiography within one month of hospitalization. group i patients showed a greater in ... | 1985 | 3878310 |
| shiga-like cytotoxin production by enteropathogenic escherichia coli serogroups. | the mechanism by which enteropathogenic escherichia coli (epec) cause disease remains to be defined. we studied epec and non-epec strains of e. coli from stool specimens obtained from infants and adults for production of shiga-like cytotoxin. although it was common for healthy infants and adults to have cytotoxin-producing e. coli as part of the fecal flora, shiga-like cytotoxin was detected more commonly and in greater amounts among epec than among other fecal e. coli. these results suggest a r ... | 1985 | 3880727 |
| brain oedema following brain ischaemia and the influence of therapy. | | 1985 | 3881111 |
| addition of three new serotypes of shigella boydii to the shigella schema. | no new serotypes have been added to the shigella schema since 1958, although several provisional serotypes have been described. we conducted biochemical and serological studies on three provisional shigella boydii serotypes. four strains of serotype 2710-54 from four widely separated countries, 7 strains of serotype 3615-53 from three different countries, and 31 strains of serotype 1344-78 (e10163) from six different countries were included. reactions of all three serotypes were consistent with ... | 1985 | 3881469 |
| an animal model of haemolytic--uraemic syndrome in shigellosis: lipopolysaccharides of shigella dysenteriae i and s. flexneri produce leucocyte-mediated renal cortical necrosis in rabbits. | to develop an animal model of the haemolytic-uraemic syndrome during shigellosis, rabbits were injected with lipopolysaccharides (lps) extracted by the hot phenol-water method from shigella dysenteriae i and from s. flexneri. two intravenous injections of lps spaced by 24 h elicited renal cortical necrosis in a generalized shwartzman reaction characterized by fibrin deposition in glomerular capillaries and by elevated plasma creatinine concentration. rabbits rendered leucopenic by busulphan trea ... | 1985 | 3882120 |
| a new provisional serovar of shigella dysenteriae. | bacterial isolates obtained in israel from stool cultures of 17 sporadic cases of acute diarrhea during the years 1972 to 1980 and from 14 patients involved in an institutional outbreak in 1984 are described. these cultures gave the biochemical reactions and pathogenicity tests characteristics of the genus shigella but failed to agglutinate, living or boiled, in any of the recognized or provisional shigella antisera. all 31 cultures were biochemically and serologically identical, and owing to th ... | 1985 | 3882749 |
| plasmid characterization of drug-resistant shigella dysenteriae 1 from an epidemic in central africa. | a widespread epidemic of severe dysentery in zaire and neighbouring central african countries was caused by a multiply drug-resistant strain of shigella dysenteriae 1. early isolations were resistant to ampicillin, chloramphenicol, streptomycin, sulphonamides and tetracyclines (r-type = acssut). later in the epidemic strains resistant to trimethoprim (tm) became prevalent and a few strains resistant to kanamycin (k) or nalidixic acid were also isolated. all resistances except nalidixic acid were ... | 1985 | 3886782 |
| shiga toxin--an expanding role in the pathogenesis of infectious diseases. | | 1985 | 3886803 |
| [role of the conjugative transfer of r-plasmids in competitive interactions between plasmid-containing and plasmid-free strains of escherichia coli in continuous culture]. | competition of the plasmid-containing strain c600 (rp4) (pbs94) of e. coli and the plasmid-free strain c600 rifr of e. coli in chemostatic and ph-static continuous cultures resulted in displacement of the plasmid-free strain in spite of its higher specific growth rate. conjugative transfer of rp4 and pbs94 plasmids into the cells of the plasmid-free strain was observed in the experiments. competition of strains c600 (pbs94) (rp4 tra-) and c600 rifr in the chemostatic culture in the absence of th ... | 1985 | 3888095 |
| properties of rat liver signal peptidase reconstituted into liposomes. | edta/kcl- or pyrophosphate-treated rough microsomes of rat liver clearly showed the co-translational cleavage of pre-human placental lactogen and translocation of the product into membrane vesicles. the signal peptidase fraction was isolated by chromatography on sephacryl s-300 of deoxycholate-treated membranes and reconstituted into liposomes by dialysis or by the biobeads sm-2 method. assay of the signal peptidase activity was performed with pre-human placental lactogen synthesized by the reti ... | 1985 | 3888971 |
| treatment of severe bacillary dysentery with trimethoprim alone. | trimethoprim (300 mg twice daily for five days) and co-trimoxazole (two tablets twice daily for five days) were compared as treatment for adult patients with severe shigellosis in rwanda. excellent bacteriological and clinical results were obtained with both regimens, with the exception of patients infected with a trimethoprim-resistant strain of shigella dysenteriae type 1. since only 20 patients were investigated, the conclusions of our study do not reach statistical significance. before recom ... | 1985 | 3890282 |
| an epidemic of bacillary dysentery at raipur. | | 1985 | 3891837 |
| enzyme immunoassay of human protein c by using monoclonal antibodies. | an enzyme-linked immunosorbent assay (elisa) for measuring human protein c by using two monoclonal antibodies directed toward the heavy chain of protein c is reported. this assay enabled the determination of protein c in concentrations of 10 to 400 ng/ml in less than 3 hours with a single antigen-antibody reaction. within-run and between-run coefficients of variation were less than 8%. the mean concentrations of protein c in plasma of 42 normal subjects, 24 patients with liver disease, 27 with d ... | 1985 | 3895562 |
| transformation of trypomastigote forms of trypanosoma cruzi into activators of alternative complement pathway by immune igg fragments. | in this report we examined the capacity of immune igg fragments to prepare trypomastigote bloodstream forms (tbf) of trypanosoma cruzi for lysis. f(ab')2 fragments were capable of presensitizing tbf for complement (c) lysis, thus excluding the participation of fc domains in the c activation process. an intact hinge region of the igg molecule was not involved either, since the corresponding fab' were almost as active as the original molecules in preparing tbf for lysis. fab also retained such act ... | 1985 | 3898353 |
| transformation of trypomastigote forms of trypanosoma cruzi into activators of alternative complement pathway by immune igg fragments. | in this report we examined the capacity of immune igg fragments to prepare trypomastigote bloodstream forms (tbf) of trypanosoma cruzi for lysis. f(ab')2 fragments were capable of presensitizing tbf for complement (c) lysis, thus excluding the participation of fc domains in the c activation process. an intact hinge region of the igg molecule was not involved either, since the corresponding fab' were almost as active as the original molecules in preparing tbf for lysis. fab also retained such act ... | 1985 | 3898353 |
| plasmid-mediated drug resistance of shigellae in kuwait. | of 153 clinical isolates of shigellae examined, 64.7% belonged to shigella flexneri, 18.9% to sh. sonnei, 11.8% to sh. boydii and 4.6% to sh. dysenteriae. part of these isolates were resistant to sulfamethoxazole and streptomycin (88.2% each), ampicillin (66.7%), tetracycline (63.4%) and co-trimoxazole (43.1%), with levels of resistance (mic50 and mic90) being invariably high. resistance to three or more drugs (multidrug resistance) was seen in 77.8% of the isolates. all the 25 strains examined ... | 1985 | 3899007 |
| shigellosis (antibiotic resistance and transfer of r-factor). | | 1985 | 3900357 |
| characterization of monoclonal antibodies against shiga-like toxin from escherichia coli. | three monoclonal antibodies, designated mab 16e6, mab 13c4, and mab 19g8, were produced which recognize shiga-like toxin (slt) from escherichia coli. all three monoclonal antibodies neutralized the cytotoxicity of e. coli slt and were able to immunoprecipitate intact labeled toxin with staphylococcus aureus protein a. the three antibodies were of the g1 heavy and kappa light chain classes. mab 16e6 bound to the b subunit of slt in western blots and also neutralized the lethality of the toxin for ... | 1985 | 3905611 |
| changing pattern of resistant shiga bacillus (shigella dysenteriae type 1) and shigella flexneri in bangladesh. | shigella dysenteriae type 1 (shiga bacillus) has made a dramatic comeback in bangladesh after 10 years when shigella flexneri was the dominant serogroup. whereas s. flexneri showed little increase in resistance to the commonly used antimicrobial agents--namely, trimethoprim-sulfamethoxazole (tmp-smx) and ampicillin-over the years, s. dysenteriae type 1 underwent rapid changes with regard to sensitivity pattern during the last two years. the first tmp-smx-resistant strain of s. dysenteriae type 1 ... | 1985 | 3905980 |
| the changing pattern of resistance to ampicillin and co-trimoxazole in shigella serotypes in bangalore, southern india. | | 1985 | 3905991 |
| [3-year study of shigellosis epidemic in rwanda, central africa. problems of public health and bacteriological aspects]. | since 1979, the lakes region (kivu in zaïre, western uganda, rwanda, burundi) has been stricken by a bacillary dysentery epidemic at s. dysenteriae type 1. supposed to be extinct since the 1950s., this bacillus has revealed a very noticeable virulence as well by the number of registered cases (524/100.000 in 1983 at ruhengeri-rwanda) as its fast break through. demographic density (from 400 to 600/km2) has played an important role and has facilitated the transition toward epidemicity. systematic ... | 1985 | 3906345 |
| determination of nanogram quantities of vanadium in biological material by isotope dilution thermal ionization mass spectrometry with ion counting detection. | | 1985 | 3907412 |
| outbreak of shigella dysenteriae type-1 dysentery in himachal pradesh--aetiologic investigation in distt: shimla. | | 1985 | 3908554 |
| [the use of an enzymatic preparation called alcalase 60 l]. | | 1985 | 3910192 |
| mechanism of sbcb-suppression of the recbc-deficiency in postreplication repair in uv-irradiated escherichia coli k-12. | the mechanism by which an sbcb mutation suppresses the deficiency in postreplication repair shown by recb recc mutants of escherichia coli was studied. the presence of an sbcb mutation in uvra recb recc cells increased their resistance to uv radiation. this enhanced resistance was not due to a suppression of the minor deficiency in the repair of dna daughter-strand gaps or to an inhibition of the production of dna double-strand breaks in uv-irradiated uvra recb recc cells; rather, the presence o ... | 1985 | 3911022 |
| plasmid characterization of shigella spp. isolated from children with shigellosis and asymptomatic excretors. | strains of shigella dysenteriae type 1 and sh. flexneri isolated during 1981-82 from children with shigellosis and also from asymptomatic excretors were examined for drug resistance and for the presence of plasmids. shigella strains isolated from dysenteric children showed multiple plasmid bands. thirty-eight percent of the strains transferred the drug resistance factor(s), either partially or fully to a recipient escherichia coli k12 strain. plasmids in the molecular weight range from between 4 ... | 1985 | 3912364 |
| [epidemiological analysis of the outbreak of bacillary dysentery in xinjiang]. | | 1985 | 3915444 |
| pressure- and temperature-dependent adhesion of pseudomonas aeruginosa to hep-2 cells. | pseudomonas aeruginosa bacteria adhere to human epitheloid (hep-2) cells in culture. under normal atmospheric conditions (1 ata), this adhesion increased significantly when the temperature rose from 22 to 37 degrees c. under hyperbaric atmosphere (= air, 7 ata) conditions, a similar, significant enhancement of bacterial adhesion to the cells was noted when the temperature rose. if the temperature was kept stable at 22 or 37 degrees c and the pressure was increased from 1 to 7 ata, a pressure-ind ... | 1985 | 3925713 |
| [virulence of shigella: epithelial cell invasiveness and other dysentery-producing factors]. | | 1985 | 3938489 |
| distribution of gamma-carboxyglutamic acid residues in partially carboxylated human prothrombins. | the role of gamma-carboxyglutamic acid in prothrombin has been examined using partially carboxylated variant prothrombins isolated from a person with a hereditary defect in vitamin k-dependent carboxylation. these species differ in gamma-carboxyglutamic acid content, distribution, and function, as monitored by metal binding properties, conformational transitions, phospholipid binding, and calcium-dependent coagulant activity (borowski, m., furie, b. c., goldsmith, g. h., and furie, b. (1985) j. ... | 1986 | 3944102 |
| distribution of gamma-carboxyglutamic acid residues in partially carboxylated human prothrombins. | the role of gamma-carboxyglutamic acid in prothrombin has been examined using partially carboxylated variant prothrombins isolated from a person with a hereditary defect in vitamin k-dependent carboxylation. these species differ in gamma-carboxyglutamic acid content, distribution, and function, as monitored by metal binding properties, conformational transitions, phospholipid binding, and calcium-dependent coagulant activity (borowski, m., furie, b. c., goldsmith, g. h., and furie, b. (1985) j. ... | 1986 | 3944102 |
| comparison of particle-associated progesterone and oxytocin in the ovine corpus luteum. | the subcellular distribution of progesterone and oxytocin within the ovine corpus luteum was investigated using differential and density gradient centrifugation. progesterone and oxytocin were associated with particles which sedimented to a density of 1.049-1.054 g/ml and 1.054-1.061 g/ml respectively. particle-associated progesterone did not, however, display physical or biochemical characteristics consistent with its storage within secretory granules. when particle-associated progesterone was ... | 1986 | 3944530 |
| characterisation of a dictyostelium discoideum dna fragment coding for a putative trnavalguu gene. evidence for a single transcription unit consisting of two overlapping class iii genes. | a genomic dna fragment from dictyostelium discoideum was characterized. this dna, although 74% d(a + t)-rich, codes for a putative trnavalguu. the trnaval gene overlaps at its 5' half with another rna polymerase iii transcription unit. this rna polymerase iii transcription unit can be folded into a trna-like shape and is comprised of significant amounts of invariant and semi-invariant nucleotides present in all eukaryotic trnas. this unit contains the two promoter blocks defined for rna polymera ... | 1985 | 3967666 |
| noninvasive measurement of pressure/volume characteristics in human limb veins by electrical admittance plethysmography. | | 1985 | 3974317 |
| effect of bromocriptine on serum pth, calcium and phosphate levels in azotemic rats. | the dopamine agonist, bromocriptine, was studied with respect to its effects on pth secretion and calcium homeostasis in sprague-dawley rats made azotemic by either total or subtotal nephrectomy. the oral or intraperitoneal administration of 0.25 mg of bromocriptine resulted in a significant increase in the serum calcium concentration when compared to animals given placebo. bromocriptine produced no significant change in the bun or the serum concentrations of creatinine, inorganic phosphate or p ... | 1985 | 3979799 |
| multiaxonal horizontal cells in the retina of the tree shrew, tupaia glis. | the retinas of most vertebrates contain two or more morphologically distinct types of horizontal cell, and usually one of these types lacks an axon. among mammals, in which two types are observed, primates are exceptional in that both types of horizontal cell have axons. it then seemed of interest to study the horizontal cells of tree shrews (tupaia glis), insectivores thought to be closely related to primates. golgi impregnations of whole, flat-preparations revealed two types of horizontal cell ... | 1985 | 3980782 |
| observations on facial nociception in a monkey after destruction of the rostral part of the trigeminal sensory nuclear complex. | facial sensibility was assessed in a cynomolgus monkey in whom the trigeminal main sensory nucleus and the rostral part of the trigeminal nucleus oralis on one side had been destroyed. the animal responded equally to noxious mechanical stimuli applied to the two sides of the face; no deficiency in nociception could be detected. this finding suggests that the synaptic connections made by nociceptor afferents from the face in rostral parts of the trigeminal sensory nuclear complex are not essentia ... | 1985 | 3982839 |
| epinephrine and norepinephrine syntheses are regulated by a glucocorticoid receptor-mediated mechanism in the bovine adrenal medulla. | the bovine adrenal medulla was investigated regarding the presence of glucocorticoid binding protein and the increases in ornithine decarboxylase (odc) activity and epinephrine and norepinephrine by dexamethasone. scatchard analysis of specific cytosol [3h] dexamethasone-binding study indicated a single class of high affinity (kd, 35 +/- 5 nm) and limited binding sites (150 +/- 26 fmoles/mg protein). competition studies of various steroids indicated a high affinity for dexamethasone and hydrocor ... | 1985 | 3990518 |
| [persistent chlamydia infection in cell culture]. | | 1985 | 3993192 |
| the acquired immunodeficiency syndrome (aids) in denmark. a report from the copenhagen study group of aids on the first 20 danish patients. | twenty danish patients with the acquired immunodeficiency syndrome (aids) had been diagnosed by january 1984, 14 of them after 1982. eighteen patients were male homosexuals, 8 of whom had visited the usa after 1979, 2 were heterosexual males with a history of sexual contacts in central africa, suggesting a transmission of aids from woman to man. aids has not been observed in drug abusers, hemophiliacs or transfused non-risk persons in denmark. the clinical picture varied according to the presenc ... | 1985 | 3993435 |
| [24-hour energy metabolism in the human: circadian rhythm, relation to body weight and nutrition]. | in 7 men with normal weight and 9 man with overweight and healthy metabolism the resting and fasting energy expenditure was indirectly calorimetrically pursued in the open system over 24 hours. the total energy expenditure over 24 hours revealed an ascertained dependence on body-weight and nutrition. the persons with overweight had a higher absolute energy expenditure, however, the activity of the energy metabolism of the body mass free from fat and the active body mass, respectively, decreased ... | 1985 | 4013439 |
| [trial of an erythrocyte antigenic diagnostic agent for detecting antibodies to coxiella burnetii]. | a new method for the preparation of q-fever erythrocyte antigenic diagnosticum, adaptable to large-scale production, has been developed, and the diagnosticum thus obtained has been found to be highly specific and sensitive. the combined use of the complement fixation and passive hemagglutination tests enhances the effectiveness of the serological study, as it not only ensures a more complete detection of antibodies to c. burnetii, phase i, in humans and cattle, but also gives more precise indica ... | 1985 | 4013575 |
| adaptation of skeletal muscle energy metabolism to repeated hypoxic-normoxic exposures and drug treatment. | muscular glycolytic fuels, intermediates and end-products (glycogen, glucose, glucose-6-phosphate, pyruvate, lactate), krebs cycle intermediates (citrate, alpha-ketoglutarate, succinate, malate), related free amino acids (glutamate, alanine), ammonia, energy store (creatine phosphate), energy mediators (atp, adp, amp) and energy charge potential were evaluated. furthermore the maximum rate (vmax) of the following muscular enzyme activities was evaluated in the crude extract and/or mitochondrial ... | 1985 | 4015259 |
| adaptation of skeletal muscle energy metabolism to repeated hypoxic-normoxic exposures and drug treatment. | muscular glycolytic fuels, intermediates and end-products (glycogen, glucose, glucose-6-phosphate, pyruvate, lactate), krebs cycle intermediates (citrate, alpha-ketoglutarate, succinate, malate), related free amino acids (glutamate, alanine), ammonia, energy store (creatine phosphate), energy mediators (atp, adp, amp) and energy charge potential were evaluated. furthermore the maximum rate (vmax) of the following muscular enzyme activities was evaluated in the crude extract and/or mitochondrial ... | 1985 | 4015259 |
| quantitative determination of tramadol in human serum by gas chromatography-mass spectrometry. | a gas chromatographic-mass spectrometric method for the quantitative determination of tramadol in human serum, plasma or whole blood samples is described. the method involves the use of [2h2, 15n]tramadol hydrochloride as an internal standard and chemical ionization with isobutane, employing single-ion monitoring for quantification. it is specific, sensitive and precise, and has high accuracy. the within-run coefficient of variation is about 1% between 25 and 200 ng/ml and 1.8-2.9% at the lowest ... | 1985 | 4019697 |
| [mechanism of the anti-arrhythmic action of phosphocreatine in acute myocardial ischemia]. | the association between phosphocreatine's antifibrillatory action and its effect on the excitement propagation processes in the ischemic area was investigated under acute coronary arterial occlusion in dogs. ischemia considerably reduced the amplitude, and increased the duration and time of onset, in local electrograms, and provoked cardiac fibrillation at the time of occlusion or during the recovery of coronary flow. a single intravenous injection of 300 mg/kg phosphocreatine eliminated cardiac ... | 1985 | 4021280 |
| [characteristics of monosynaptic connections between neurons of the callosal system and specific thalamic nuclei]. | in the sensorimotor cortex of awake rabbits monosynaptic afferent and efferent connections of callosal neurons (cn) with ipsilateral specific thalamic nuclei (ventral posterolateral, ventral posteromedial, ventral lateral and anteroventral nuclei) were analyzed by electrophysiological method and compared with those of target-units of callosal fibres (tu). it was demonstrated that cn and tu differed in their monosynaptic inputs from the thalamus and in their axons projecting to these structures a ... | 1985 | 4022178 |
| [characteristics of a cell line obtained from a human hypernephroma]. | a stable tumorigenic cell line has been obtained from the human hypernephrome. the culture grew as monolayers of large polygonal cells with foamy cytoplasm and 1-2 nucleoli. the cells formed monolayer after the third day after planting into flask and then formed multilayered growth. the electron microscopic studies of hypernephrome sections revealed the dark and light cells. the hypernephrome cells have characteristics of tumor cells. on the 200th passage the cells are aneuploid with a model num ... | 1985 | 4027291 |
| presaccadic 'spike' potential: investigation of topography and source. | approximately 15-30 ms before a saccade, a large-amplitude, relatively short duration potential whose source has not previously been determined with certainty, can be recorded from the scalp in man. we have measured the surface topography of this potential during horizontal saccades in normal subjects and have found it to be maximal near the eye on the side ipsilateral to the direction of the saccade. dipole modelling predicts a source near the eye ipsilateral to the direction of gaze, and the a ... | 1985 | 4027625 |
| presaccadic 'spike' potential: investigation of topography and source. | approximately 15-30 ms before a saccade, a large-amplitude, relatively short duration potential whose source has not previously been determined with certainty, can be recorded from the scalp in man. we have measured the surface topography of this potential during horizontal saccades in normal subjects and have found it to be maximal near the eye on the side ipsilateral to the direction of the saccade. dipole modelling predicts a source near the eye ipsilateral to the direction of gaze, and the a ... | 1985 | 4027625 |
| rapid extraction and purification of diethylstilboestrol in bovine urine hydrolysates using reversed-phase c18 columns before determination by radioimmunoassay. | | 1985 | 4030972 |
| systemic complement activation, lung injury, and products of lipid peroxidation. | previously we have demonstrated that systemic activation of the complement system after intravenous injection of cobra venom factor (cvf) results in acute lung injury as reflected by increases in the vascular permeability of the lung as well as by morphologic evidence of damage to lung vascular endothelial cells. in using the vascular permeability of the lung as the reference, the current studies show a quantitative correlation between lung injury and the appearance in plasma of lipid peroxidati ... | 1985 | 4031060 |
| role of uricase in the triggering of germination of bacillus fastidiosus spores. | the likelihood that uric acid was the only compound capable of triggering germination of bacillus fastidiosus spores was reinforced by the finding that ureidoglycollic acid, urea, nh4cl, 2,8-dihydroxypurine and a combination of l-alanine and o-carbamoyl-d-serine were ineffective as germinants. uric acid-triggered germination of b. fastidiosus was prevented by a range of inhibitors that also inhibited uricase activity in dormant spore extracts. o2 uptake during germination started immediately aft ... | 1985 | 4038258 |
| three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. v. assignment of actin in the actin-tropomyosin-myosin subfragment-1 complex. | to assign the actin molecule in the three-dimensional image of the actin-tropomyosin-myosin subfragment-1 (actin-tm-s1) complex, the three-dimensional image of the actin-tropomyosin complex was correlated to that of actin-tm-s1. to assess the similarity of two structures in a quantitative manner, we used a normalized cross-correlation function ("similarity function"). the calculation of similarity indicated that domain a and domain b defined in (1, 2) correspond to actin-tropomyosin. this assign ... | 1985 | 4039721 |
| local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males. | the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ... | 1985 | 4040921 |
| [mechanical injector for angiography]. | | 1985 | 4051773 |
| determination of zinc in whole blood, plasma and serum using zeeman effect flame atomic absorption spectroscopy. | methods are presented for the determination of zinc in whole blood, plasma and serum using zeeman effect flame atomic absorption spectroscopy and a flame microsampling funnel. whole blood was diluted 1/25 with 0.10 mol/l hydrochloric acid; plasma and serum were diluted 1/5 with deionized water. concentrations could be read directly from standards prepared in human blood pools. the within-run relative standard deviation (rsd) was 0.50%, 0.82% and 0.61% for whole blood specimens with concentration ... | 1985 | 4053384 |
| suppression of human lymphocyte mitogen response by retroviruses of type d. ii. non-activity of mason-pfizer monkey virus versus activity of human cell line derived virus pmfv. | in contrast to the human cell line derived type d retrovirus pmfv, the mason-pfizer monkey virus (mpmv) does not suppress the mitogen response of normal human lymphocytes. both viruses have been propagated on the same cell lines and purified by the same methods. mpmv did not contain a factor able to abolish pmfv-induced suppression of the mitogen response. neither could mpmv suppress the mitogen response of lymphocytes from rhesus monkeys or baboons. pmfv however inhibited their reactivity. | 1985 | 4062558 |
| classically conditioned hyperglycemia in the obese mouse. | the obese (c57bl/6j ob/ob) mouse is a commonly used animal model of non-insulin-dependent diabetes mellitus. it has recently been demonstrated that this mouse is not consistently hyperglycemic, however, unless it is subjected to environmental stress. in the present study, hyperglycemia in obese mice was induced by classical conditioning. obese diabetic mice and lean control animals were exposed to shaking stress. all animals developed hyperglycemia in response to shaking. to demonstrate classica ... | 1985 | 4070525 |
| motor conduction velocity in the human spinal cord: slowed conduction in multiple sclerosis and radiation myelopathy. | transcutaneous electrical stimulation of the central nervous system was used to measure motor conduction velocity in the human spinal cord in 21 subjects aged 22 to 75 years (mean 55 years), none of whom had neurological disease. the motor conduction velocity between the sixth cervical (c6) and first lumbar (l1) vertebral levels was 67.4 +/- 9.1 m/s. this probably represents conduction velocity in the corticospinal tracts. in these subjects the motor conduction velocity in the cauda equina, betw ... | 1985 | 4078579 |
| alu sequences transcription in x. laevis oocytes: nuclear-cytoplasmic partitioning and evidence for 3' end processing reactions. | a large alu-family cluster in the 5' flanking region of a human alpha 1-acid glycoprotein gene has been identified and sequenced. individual members microinjected into x. laevis oocytes are transcribed only when canonical box a and b components of the split pol iii promoter are present. alu transcripts accumulate in the nucleus. an unusually short alu transcript, able to assume a stable secondary structure, undergoes a 3' end processing reaction similar to the one required for trna 3' end matura ... | 1985 | 4080545 |
| inhibition of histamine-n-methyltransferase activity by neuromuscular blocking drugs. | on the basis of previous findings that histamine-n-methyltransferase (hmt) activity can be significantly enhanced or inhibited by a number of analogues of histamine and drugs containing dialkylaminoalkyl moieties, we investigated whether the neuromuscular blocking drugs alcuronium, d-tubocurarine, decamethonium, succinylcholine, gallamine and pancuronium each of which contain quaternary ammonium groups, influence the activity of hmt. our findings showed that all six drugs significantly inhibited ... | 1985 | 4083176 |
| fourteen years of shigellosis in dhaka: an epidemiological analysis. | we examined whether the proportion of shigellae patients among diarrhoeal cases, the distribution, species, case-fatality rates and hospital visits changed over time in dhaka. we isolated 19639 shigella strains from 822812 diarrhoea cases treated at the international centre for diarrhoeal disease research, bangladesh (icddr,b), between 1969 and 1982. the number of cases increased from 209 (2.5%) in 1969 to 4833 (7.7%) in 1976. extrapolating from a 4% vigorous systematic sample of icddr,b hospita ... | 1985 | 4086147 |
| [ultrastructural aspects of the wound healing process of the skin of albino rats]. | | 1985 | 4089424 |
| amino acid and protein metabolism in dorsal root ganglia of rabbits with experimental allergic neuritis. | amino acid and protein metabolism has been studied in the dorsal root ganglia of rabbits with experimental allergic neuritis (ean). the concentrations of a number of nonessential amino acids (glutamine, serine, aspartate, and glutamate) were reduced in the spinal ganglia of ean animals without any comparable change in the blood plasma. the short-term influx of glycine and gaba was decreased in ean animals, whereas that of histidine and valine was not altered. the prolonged accumulation of all th ... | 1985 | 4094728 |
| dysentery epidemic in central america. | | 1970 | 4097604 |
| [study of genetic control of synthesis of the typospecificity in shigella antigens of the subgroup b]. | | 1969 | 4098562 |
| [fine structure of shigella sonnei, flexneri and boydii phages]. | | 1969 | 4098712 |
| structure of the corneal stroma. | | 1970 | 4099087 |