| immunological and pathological changes in the placenta during infection with listeria monocytogenes in pregnant guinea pigs. | exposure to listeria monocytogenes during pregnancy can result in spontaneous abortion and stillbirths; however, the mechanisms are unknown. our objective was to determine the effects of infection on specific inflammatory and anti-inflammatory cytokine mrna expression and apoptosis in the placenta after infection with l. monocytogenes. pregnant guinea pigs were treated on gestation day (gd) 35 with 10(8) colony forming units l. monocytogenes and sacrificed on gd 37, 41, 44, or 55. at gd 41, ifn- ... | 2008 | 18793713 |
| a survey of laboratory-confirmed isolates of invasive listeriosis in israel, 1997-2007. | during the 11-year period from 1997 to 2007, 321 isolates of listeria monocytogenes from sporadic cases of invasive listeriosis were reported to the national reference laboratory in israel. of these isolates, 113 (35%) were identified from perinatal cases, and 208 (65%) from non-perinatal cases. the prevalent serovars were 4b, 1/2b, 1/2a and 4c. serovar 4b was identified in 80.5% of the perinatal isolates (p=0.0162), while the number of 1/2b and 1/2a strains increased in the > or = 60 years old ... | 2009 | 18796168 |
| antimicrobial effects of turkish propolis, pollen, and laurel on spoilage and pathogenic food-related microorganisms. | the antimicrobial activities of propolis extract, pollen extract, and essential oil of laurel (laurus nobilis l.) at concentrations from 0.02% to 2.5% (vol/vol) were investigated on bacteria (bacillus cereus, bacillus subtilis, escherichia coli, salmonella typhimurium, staphylococcus aureus, yersinia enterocolitica, enterococcus faecalis, and listeria monocytogenes), yeasts (saccharomyces cerevisiae and candida rugosa), and molds (aspergillus niger and rhizopus oryzae). pollen has no antimicrobi ... | 2008 | 18800911 |
| pillars article: development of th1 cd4+ t cells through il-12 produced by listeria-induced macrophages. 1993. science 260(5107): 547-549. | | 2008 | 18802044 |
| infection of myeloid dendritic cells with listeria monocytogenes leads to the suppression of t cell function by multiple inhibitory mechanisms. | myeloid dendritic cells (dc) and macrophages play an important role in pathogen sensing and antimicrobial defense. in this study we provide evidence that myeloid dc respond to infection with listeria monocytogenes with simultaneous induction of multiple stimulatory and inhibitory molecules. however, the overall impact of infected dc during t cell encounter results in suppression of t cell activation, indicating that inhibitory pathways functionally predominate. inhibitory activity of infected dc ... | 2008 | 18802101 |
| antimicrobial efficacy of citron essential oil on spoilage and pathogenic microorganisms in fruit-based salads. | the aim of this study was the evaluation of the effects of pure citral and citron essential oil on microbial spoilage and growth and survival of pathogenic microorganisms during storage. they were added in the syrup of industrial ready-to-eat fruit salads stored at 9 degrees c. both citral (25 to 125 ppm) and citron essential oil (300, 600, 900 ppm) were able to prolong the microbial shelf life of the fruit-based salads. the essential oil gave excellent results, avoiding the undesirable effects ... | 2008 | 18803716 |
| resistance of nutrient-deprived listeria monocytogenes 10403s and a deltasigb mutant to chemical stresses in the presence or absence of oxygen. | nutrient-deprived listeria monocytogenes have increased resistance to processing control measures. heat-stressed l. monocytogenes cells produce higher counts under anaerobic conditions and sigb reportedly contributes to the survival of environmentally stressed gram-positive bacteria. in this study, a wild type (wt) strain, l. monocytogenes 10403s, and a deltasigb mutant, fsla1-254, were stressed by starvation in phosphate buffered saline coupled with exposure to chemicals with/without oxygen. in ... | 2008 | 18803717 |
| wasp identity theft by a bacterial effector. | espf(u), a protein secreted by pathogenic enterohaemorrhagic e. coli (ehec), activates n-wasp/wasp to induce actin pedestal formation in host cells. two recent papers in nature show that espf(u) exploits a wasp activation strategy so extreme that it may effectively sequester wasp, blinding it to both autoinhibition and cellular regulation. | 2008 | 18804428 |
| gene expression profiling of listeria monocytogenes strain f2365 during growth in ultrahigh-temperature-processed skim milk. | to study how listeria monocytogenes survives and grows in ultrahigh-temperature-processed (uht) skim milk, microarray technology was used to monitor the gene expression profiles of strain f2365 in uht skim milk. total rna was isolated from strain f2365 in uht skim milk after 24 h of growth at 4 degrees c, labeled with fluorescent dyes, and hybridized to "custom-made" commercial oligonucleotide (35-mers) microarray chips containing the whole genome of l. monocytogenes strain f2365. compared to l. ... | 2008 | 18806004 |
| identification of components of the sigma b regulon in listeria monocytogenes that contribute to acid and salt tolerance. | sigma b (sigma(b)) is an alternative sigma factor that controls the transcriptional response to stress in listeria monocytogenes and is also known to play a role in the virulence of this human pathogen. in the present study we investigated the impact of a sigb deletion on the proteome of l. monocytogenes grown in a chemically defined medium both in the presence and in the absence of osmotic stress (0.5 m nacl). two new phenotypes associated with the sigb deletion were identified using this mediu ... | 2008 | 18806006 |
| inflammation and autoimmunity caused by a shp1 mutation depend on il-1, myd88, and a microbial trigger. | a recessive phenotype called spin (spontaneous inflammation) was induced by n-ethyl-n-nitrosourea (enu) mutagenesis in c57bl/6j mice. homozygotes display chronic inflammatory lesions affecting the feet, salivary glands and lungs, and antichromatin antibodies. they are immunocompetent and show enhanced resistance to infection by listeria monocytogenes. tlr-induced tnf and il-1 production are normal in macrophages derived from spin mice. the autoinflammatory phenotype of spin mice is fully suppres ... | 2008 | 18806225 |
| conjugated action of two species-specific invasion proteins for fetoplacental listeriosis. | the ability to cross host barriers is an essential virulence determinant of invasive microbial pathogens. listeria monocytogenes is a model microorganism that crosses human intestinal and placental barriers, and causes severe maternofetal infections by an unknown mechanism. several studies have helped to characterize the bacterial invasion proteins inla and inlb. however, their respective species specificity has complicated investigations on their in vivo role. here we describe two novel and com ... | 2008 | 18806773 |
| immunosensitization with a bcl-2 small molecule inhibitor. | several tumor immunotherapy approaches result in a low percentage of durable responses in selected cancers. we hypothesized that the insensitivity of cancer cells to immunotherapy may be related to an anti-apoptotic cancer cell milieu, which could be pharmacologically reverted through the inhibition of antiapoptotic bcl-2 family proteins in cancer cells. abt-737, a small molecule inhibitor of the antiapoptotic proteins bcl-2, bcl-w and bcl-x(l), was tested for the ability to increase antitumor i ... | 2009 | 18807035 |
| cytoplasmic delivery of antigens, by bacillus subtilis enhances th1 responses. | endospores of the gram-positive bacterium, bacillus subtilis, have been used successfully for delivery of antigens where the immunogen is expressed on the spore surface. in this work the spore has been engineered to deliver antigens to the cytoplasm of macrophages by expressing listeriolysin o (llo) or a derivative, llo(l461t), that is stable at neutral ph, from the b. subtilis vegetative cell. following phagocytosis spores were shown to germinate in the phagosome enabling secretion of llo/llo(l ... | 2008 | 18809450 |
| effects of irradiation on survival and growth of listeria monocytogenes and natural microflora in vacuum-packaged turkey hams and breast rolls. | the effect of electron beam irradiation on the survival and growth of listeria monocytogenes and natural microflora in oven-roasted turkey breast rolls and turkey hams was evaluated. slices of turkey breast rolls and hams were inoculated with a 5-strain mixture of l. monocytogenes to achieve 10(6) to 10(7) cfu/cm2; vacuum-packaged; irradiated at 0 (control), 1.0, 1.5, 2.0, or 2.5 kgy; and stored at 4 degrees c for up to 28 d. numbers of naturally occurring bacteria on sliced turkey hams and brea ... | 2008 | 18809878 |
| the bradykinin b2 receptor in the early immune response against listeria infection. | the endogenous danger signal bradykinin was recently found implicated in the development of immunity against parasites via dendritic cells. we here report an essential role of the b(2) (b(2)r) bradykinin receptor in the early immune response against listeria infection. mice deficient in b(2)r (b(2)r(-/-) mice) were shown to suffer from increased hepatic bacterial burden and concomitant dramatic weight loss during infection with listeria monocytogenes. levels of cytokines known to play a pivotal ... | 2009 | 18810490 |
| evaluating the growth of listeria monocytogenes in refrigerated ready-to-eat frankfurters: influence of strain, temperature, packaging, lactate and diacetate, and background microflora. | this research was conducted to study the growth of listeria monocytogenes inoculated on frankfurters stored at different conditions as a basis for a safety-based consume by shelf life date label. three l. monocytogenes strains were separately inoculated at 10 to 20 cfu/cm2 onto frankfurters that were previously formulated with or without high pressure and with or without added 2% potassium lactate (pl) and 0.2% sodium diacetate (sd). inoculated frankfurters were air or vacuum packaged; stored at ... | 2008 | 18810864 |
| competitiveness and antibacterial potential of bacteriocin-producing starter cultures in different types of fermented sausages. | application of bacteriocin-producing starter cultures of lactic acid bacteria in fermented sausage production contributes to food safety. this is sometimes hampered by limited efficacy in situ and by uncertainty about strain dependency and universal applicability for different sausage types. in the present study, a promising antilisterial-bacteriocin producer, lactobacillus sakei ctc 494, was applied as a coculture in addition to commercial fermentative starters in different types of dry-ferment ... | 2008 | 18810865 |
| dynamic modeling of listeria monocytogenes growth in pasteurized vanilla cream after postprocessing contamination. | a product-specific model was developed and validated under dynamic temperature conditions for predicting the growth of listeria monocytogenes in pasteurized vanilla cream, a traditional milk-based product. model performance was also compared with growth predictor and sym'previus predictive microbiology software packages. commercially prepared vanilla cream samples were artificially inoculated with a five-strain cocktail of l. monocytogenes, with an initial concentration of 102 cfu g(-1), and sto ... | 2008 | 18810866 |
| modeling survival of listeria monocytogenes in the traditional greek soft cheese katiki. | in the present work, survival of listeria monocytogenes in the traditional greek soft, spreadable cheese katiki was studied throughout the shelf life of the product. samples of finished cheese were inoculated with a cocktail of five l. monocytogenes strains (ca. 6 log cfu g(-1)) and stored at 5, 10, 15, and 20 degrees c. acid-stress adaptation or cross-protection to the same stress was also investigated by inoculation of acid-adapted cells in the product. the results showed that pathogen surviva ... | 2008 | 18810867 |
| efficacy of plant essential oils against foodborne pathogens and spoilage bacteria associated with ready-to-eat vegetables: antimicrobial and sensory screening. | the objectives of this study were to evaluate the antimicrobial activity of plant essential oils (eos) against foodborne pathogens and key spoilage bacteria pertinent to ready-to-eat vegetables and to screen the selected eos for sensory acceptability. the eos basil, caraway, fennel, lemon balm, marjoram, nutmeg, oregano, parsley, rosemary, sage, and thyme were evaluated. the bacteria evaluated were listeria spp., staphylococcus aureus, lactobacillus spp., bacillus cereus, salmonella, enterobacte ... | 2008 | 18810868 |
| enhanced inactivation of foodborne pathogenic and spoilage bacteria by fd&c red no. 3 and other xanthene derivatives during ultrahigh pressure processing. | variability among microorganisms in barotolerance has been demonstrated at genus, species, and strain levels. identification of conditions and additives that enhance the efficacy of ultrahigh pressure (uhp) against important foodborne microorganisms is crucial for maximizing product safety and stability. preliminary work indicated that fd&c red no. 3 (red 3), a xanthene derivative, was bactericidal and acted synergistically with uhp against lactobacillus spp. the objective of this study was to d ... | 2008 | 18810870 |
| modeling the physiological state of the inoculum and co2 atmosphere on the lag phase and growth rate of listeria monocytogenes. | in previous studies, the growth of l. monocytogenes has been modeled under different co2 headspace concentrations; however, the inoculum cells were always in the stationary phase. in this study, the growth of l. monocytogenes under different co2 concentrations as affected by the physiological state of the cells was investigated. exponential-growth-phase, stationary-phase, dried, and starved cells were prepared and inoculated at 5 degrees c into brain heart infusion broths that had been preequili ... | 2008 | 18810878 |
| antibacterial activity of xanthorrhizol isolated from curcuma xanthorrhiza roxb. against foodborne pathogens. | xanthorrhizol, isolated from the ethanol extract of curcuma xanthorrhiza roxb., is a sesquiterpene compound with a molecular weight of 218. the aim of this study was to investigate the antibacterial activity of xanthorrhizol against foodborne pathogens. the antibacterial activity of xanthorrhizol was measured in terms of the mic and the mbc. mics and mbcs of xanthorrhizol against bacillus cereus, clostridium perfringens, listeria monocytogenes, staphylococcus aureus, salmonella typhimurium, and ... | 2008 | 18810881 |
| microbial and sensory characteristics of camel meat during refrigerated storage as affected by gamma irradiation. | the present study was undertaken to assess the microbiological profile of fresh camel meat and the possibility of improving microbial quality and extending the refrigerated storage life of meat by using low-dose gamma irradiation. camel meat samples were subjected to 0 (control), 1.5 and 3 kgy doses and stored at 3 +/- 1 degrees c. the microbial and sensory attributes were evaluated. exposure to 1.5 kgy dose significantly reduced the initial counts of aerobic plate counts (apcs), psychrophilic b ... | 2008 | 18814652 |
| gilt is a critical host factor for listeria monocytogenes infection. | listeria monocytogenes is a gram-positive, intracellular, food-borne pathogen that can cause severe illness in humans and animals. on infection, it is actively phagocytosed by macrophages; it then escapes from the phagosome, replicates in the cytosol, and subsequently spreads from cell to cell by a non-lytic mechanism driven by actin polymerization. penetration of the phagosomal membrane is initiated by the secreted haemolysin listeriolysin o (llo), which is essential for vacuolar escape in vitr ... | 2008 | 18815593 |
| protein expression by listeria monocytogenes grown on a rte-meat matrix. | little is known about whether the growth of l. monocytogenes on a ready-to-eat (rte) meat matrix has an impact on the bacterium's pathogenic capabilities. in this report, we examined protein expression by l. monocytogenes grown on rte sliced turkey meat, using l. monocytogenes grown on brain-heart-infusion agar as a control. total protein fractions of l. monocytogenes from both growth conditions were extracted and compared by two-dimensional gel electrophoresis. seventy-seven proteins expressed ... | 2008 | 18817996 |
| mechanism of synergistic inhibition of listeria monocytogenes growth by lactic acid, monolaurin, and nisin. | the combined lactic acid, monolaurin, and nisin effects on time-to-detection (optical density at 600 nm) extension were greater (p < 0.05) than any single or paired combination effect, which demonstrates a synergistic interaction among the antimicrobials. monolaurin exposure caused c12:0 cell membrane incorporation. lactic acid caused increased monolaurin c12:0 membrane incorporation, while nisin had no influence. we postulate that lactic acid-enhanced monolaurin c12:0 incorporation into the cel ... | 2008 | 18820062 |
| chronic ethanol induces inhibition of antigen-specific cd8+ but not cd4+ immunodominant t cell responses following listeria monocytogenes inoculation. | chronic ethanol consumption results in immunodeficiency. previous work with chronic ethanol-fed mice has shown reduced splenic weight and cellularity, including reduced numbers of cd8+ t cells. however, antigen-specific cd8+ and cd4+ t cell responses in chronic ethanol-fed mice have been studied relatively little. we have used an attenuated listeria monocytogenes strain dpl 1942 (lm deltaacta) to inoculate mice and subsequently used cd4+ and cd8+ immunodominant peptides of lm to measure the cd4+ ... | 2009 | 18820175 |
| yersinia enterocolitica yop mutants as oral live carrier vaccines. | attenuated enteropathogenic yersiniae that translocate heterologous antigens into the cytosol of antigen presenting cells via their type three secretion system (ttss) are considered promising candidates for the development of live oral vaccine carrier strains that induce cd8 t cell responses. wild type yersinia enterocolitica of serotype o:8 however efficiently suppresses the immune response of the host by translocating effector proteins called yersinia outer proteins (yops) into the cytosol of ... | 2008 | 18822332 |
| frontiers in immunology research network--2008 international conference. | | 2008 | 18828067 |
| effect of gamma radiation on heat shock protein expression of four foodborne pathogens. | the effects of gamma radiation on three heat shock proteins (hsps) (groel, dnak and groes) synthesis in two gram-negative (escherichia coli and salmonella serotype typhimurium) and two gram-positive (staphylococcus aureus and listeria monocytogenes) bacteria were investigated. | 2008 | 18828787 |
| [listeria and listeriosis: from farm to fork]. | listeria monocytogenes is a bacterium widely spread in the environment. its persistence in industrial environment leads to food product contamination from the raw materials and constitutes a recurrent problem in food processing industry despite the use of cold chain procedures. l. monocytogenes is a foodborne pathogen causing severe and life-threatening infections that evolve mainly under sporadic mode, even if epidemics sometimes occur. listeriosis causes mainly septicemia, central nervous syst ... | 2009 | 18829183 |
| cancer immunotherapy targeting the high molecular weight melanoma-associated antigen protein results in a broad antitumor response and reduction of pericytes in the tumor vasculature. | the high molecular weight melanoma-associated antigen (hmw-maa), also known as melanoma chondroitin sulfate proteoglycan, has been used as a target for the immunotherapy of melanoma. this antigen is expressed on the cell surface and has a restricted distribution in normal tissues. besides its expression in a broad range of transformed cells, this antigen is also found in pericytes, which are important for tumor angiogenesis. we generated a recombinant listeria monocytogenes (lm-llo-hmw-maa-c) th ... | 2008 | 18829565 |
| endoplasmic reticulum stress regulator xbp-1 contributes to effector cd8+ t cell differentiation during acute infection. | the transcription factor x-box-binding protein-1 (xbp-1) plays an essential role in activating the unfolded protein response in the endoplasmic reticulum (er). transcribed xbp-1 mrna is converted to its active form by unconventional cytoplasmic splicing mediated by inositol-requiring enzyme-1 (ire-1) upon er stress. we report activation of the ire-1/xbp-1 pathway in effector cd8(+) t cells during the response to acute infection. transcription of unspliced xbp-1 mrna is up-regulated by il-2 signa ... | 2008 | 18832700 |
| [bacteriological evaluation of goat milk and cheese distributed in the metropolitan area of san josé, costa rica]. | in the last years, there has been an increase in the production, industrialization and consumption of goat's milk and derivate products, including cheese, worldwide. nevertheless, in costa rica there is no study of these products, reason why the objective of this work was to determine the microbiological characteristics of goat's milk and fresh cheese distributed in the metropolitan area of san josé, costa rica, in order to evaluate its impact in the economical field and as a potential risk for ... | 2008 | 18833996 |
| a novel dna microarray for rapid diagnosis of enteropathogenic bacteria in stool specimens of patients with diarrhea. | a microarray technique for the detection and identification of enteropathogenic bacteria at the species and subspecies levels was developed in this study, and the target bacteria included pathogenic escherichia coli, vibrio cholerae, vibrio parahaemolyticus, salmonella enterica, campylobacter jejuni, shigellae, yersinia enterocolitica, and listeria monocytogenes. the virulence gene of each pathogen was chosen as the amplification target, labeled with a fluorescence dye by multiplex polymerase ch ... | 2008 | 18834908 |
| pulsed-field gel electrophoresis (pfge) analysis of listeria monocytogenes isolates from different sources and geographical origins and representative of the twelve serovars. | multiplex-pcr (mpcr) serogrouping and pulsed-field gel electrophoresis (pfge) subtyping analysis are currently used by several public and private laboratories for the characterization of listeria monocytogenes. in this study a set of 80 l. monocytogenes isolates belonging to the twelve serovars was used to investigate (i) the typeability of the rare serovars, (ii) the ability of pfge analysis with apai and asci to differentiate serovars within mpcr serogroups and (iii) the association of molecul ... | 2008 | 18835121 |
| a putative abc transporter is involved in negative regulation of biofilm formation by listeria monocytogenes. | listeria monocytogenes may persist for long periods in food processing environments. in some instances, this may be due to aggregation or biofilm formation. to investigate the mechanism controlling biofilm formation in the food-borne pathogen l. monocytogenes, we characterized lm-49, a mutant with enhanced ability of biofilm formation generated via transposon tn917 mutagenesis of l. monocytogenes 4b g. in this mutant, a tn917 insertion has disrupted the coding region of the gene encoding a putat ... | 2008 | 18836003 |
| comparative analysis of extracellular and intracellular proteomes of listeria monocytogenes strains reveals a correlation between protein expression and serovar. | listeria monocytogenes, the etiologic agent of listeriosis, remains a serious public health concern, with its frequent occurrence in food environments coupled with a high mortality rate. among the 13 serovars, human listeriosis is mostly associated with the serovar 4b, 1/2b, and 1/2a strains. to investigate the diversity of l. monocytogenes, the intracellular and extracellular proteins of 12 strains were analyzed by two-dimensional gel electrophoresis. these strains had different origins, belong ... | 2008 | 18836007 |
| development and implementation of a multiplex single-nucleotide polymorphism genotyping assay for detection of virulence-attenuating mutations in the listeria monocytogenes virulence-associated gene inla. | the virulence factor internalin a (inla) facilitates the uptake of listeria monocytogenes by epithelial cells that express the human isoform of e-cadherin. previous studies identified naturally occurring premature stop codon (pmsc) mutations in inla and demonstrated that these mutations are responsible for virulence attenuation. we assembled >1,700 l. monocytogenes isolates from diverse sources representing 90 ecori ribotypes. a subset of this isolate collection was selected based on ribotype fr ... | 2008 | 18836010 |
| immunomagnetic separation and pcr detection of listeria monocytogenes in turkey meat and antibiotic resistance of the isolates. | 1. conventional cultivation and immunomagnetic separation (ims) cultivation methods were compared for the isolation specificity and sensitivity of l. monocytogenes from turkey meat samples. pcr was used to confirm the isolates. disc diffusion was performed to determine the antibiotic susceptibility profiles. a total of 180 turkey meat samples collected from markets in turkey were tested. 2. l. monocytogenes was detected in 23 samples (12.7%) by ims and conventional cultivation. it was isolated f ... | 2008 | 18836902 |
| bacteriophage therapy and the mutant selection window. | we use kinetic models to investigate how to design antimicrobial phage therapies to minimize emergence of resistant bacteria. we do this by modifying the "mutant selection window" hypothesis in a way that accounts for the ongoing self-replication of the phage. we show that components of combination phage therapies need to be appropriately matched if treatment is to avoid the emergence of resistant bacteria. matching of components is more easily achieved when phage dosages are high enough that on ... | 2008 | 18838590 |
| birth-and-death evolution of the internalin multigene family in listeria. | the birth-and-death model of multigene family evolution describes patterns of gene origination, diversification and loss within multigene families. since it was first developed in the 1990s, the model has been found to characterize a large number of eukaryotic multigene families. in this paper, we report for the first time a bacterial multigene family that undergoes birth-and-death evolution. by analyzing the evolutionary relationships among internalins, a relatively large and diverse family of ... | 2008 | 18840511 |
| lineage specific recombination rates and microevolution in listeria monocytogenes. | the bacterium listeria monocytogenes is a saprotroph as well as an opportunistic human foodborne pathogen, which has previously been shown to consist of at least two widespread lineages (termed lineages i and ii) and an uncommon lineage (lineage iii). while some l. monocytogenes strains show evidence for considerable diversification by homologous recombination, our understanding of the contribution of recombination to l. monocytogenes evolution is still limited. we therefore used structure and c ... | 2008 | 18842152 |
| enhancing bile tolerance improves survival and persistence of bifidobacterium and lactococcus in the murine gastrointestinal tract. | the majority of commensal gastrointestinal bacteria used as probiotics are highly adapted to the specialised environment of the large bowel. however, unlike pathogenic bacteria; they are often inadequately equipped to endure the physicochemical stresses of gastrointestinal (gi) delivery in the host. herein we outline a patho-biotechnology strategy to improve gastric delivery and host adaptation of a probiotic strain bifidobacterium breve ucc2003 and the generally regarded as safe (gras) organism ... | 2008 | 18844989 |
| outbreak of listeria monocytogenes infections associated with pasteurized milk from a local dairy--massachusetts, 2007. | on november 27, 2007, a local health officer in central massachusetts contacted the massachusetts department of public health (mdph) to report listeriosis in a man aged 87 years. pulsed-field gel electrophoresis (pfge) performed on the patient's listeria monocytogenes isolate produced a pattern indistinguishable from that of isolates from three other cases identified in residents of central massachusetts in june, october, and early november 2007. mdph, in collaboration with local public health o ... | 2008 | 18846031 |
| characterization of human invasive isolates of listeria monocytogenes in sweden 1986-2007. | since 1986, 68% of the listeria monocytogenes isolates from human cases of invasive listeriosis in sweden are available for retrospective studies. the aim of the present study was to characterize 601 human invasive isolates of l. monocytogenes in sweden from 1986 to 2007 by using serotyping and pulsed-field gel electrophoresis. since 1996, serovar 4b was permanently reduced to the second or third most common serovar in human cases in sweden. during the latter period, 2000-2007, only 13% belonged ... | 2008 | 18847381 |
| precut prepackaged lettuce: a risk for listeriosis? | the most recent outbreaks of listeriosis have been traced back to contaminated ready-to-eat (rte) poultry and meat products. however, listeria monocytogenes can be isolated from every food group, including fresh vegetables. this review is focused on one of the most popular rte vegetable products, precut prepackaged lettuce. the available literature concerning listeria contamination of vegetables is reviewed, and possible reasons why no recent outbreaks or sporadic cases of listeriosis due to con ... | 2008 | 18847382 |
| the tsc-mtor signaling pathway regulates the innate inflammatory response. | the innate inflammatory immune response must be tightly controlled to avoid damage to the host. here, we showed that the tuberous sclerosis complex-mammalian target of rapamycin (tsc-mtor) pathway regulated inflammatory responses after bacterial stimulation in monocytes, macrophages, and primary dendritic cells. inhibition of mtor by rapamycin promoted production of proinflammatory cytokines via the transcription factor nf-kappab but blocked the release of interleukin-10 via the transcription fa ... | 2008 | 18848473 |
| dynamic regimes and bifurcations in a model of actin-based motility. | propulsion by actin polymerization is widely used in cell motility. here, we investigate a model of the brush range of an actin gel close to a propelled object, describing the force generation and the dynamics of the propagation velocity. we find transitions between stable steady states and relaxation oscillations when the attachment rate of actin filaments to the obstacle is varied. the oscillations set in at small values of the attachment rate via a homoclinic bifurcation. a second transition ... | 2008 | 18851073 |
| colony-stimulating factor-1-dependent macrophage functions regulate the maternal decidua immune responses against listeria monocytogenes infections during early gestation in mice. | the association between extreme-prematurity births and intrauterine infection emphasizes the importance of understanding the host immune responses against uterine-invading microbes during early pregnancy to the prevention of preterm births. listeria monocytogenes, a clinically relevant intracellular bacterium, has a predilection for replication at the maternofetal interface during pregnancy. here, using mice carrying the recessive null osteopetrotic mutation in the colony-stimulating factor-1 (c ... | 2009 | 18852237 |
| listeria monocytogenes: maternal-foetal infections in denmark 1994-2005. | maternal-foetal infection by listeria monocytogenes is a rare complication in pregnancy. in the period 1994-2005, 37 culture-confirmed cases of maternal-foetal listeria monocytogenes infections were reported in denmark. we examined 36 patients' files in order to evaluate risk factors, clinical and laboratory findings, response to therapy, and outcome for maternal-foetal listeriosis. patient data and bacteriological findings were divided into 2 groups for comparison: 1 group with children born al ... | 2009 | 18855227 |
| structural bioinformatics study of pnp from listeria monocytogenes. | this work describes for the first time a model of purine nucleoside phosphorylase from listeria monocytogenes (lmpnp). we modeled the complexes of lmpnp with ligands in order to determine the structural basis for specificity. comparative analysis of the model of lmpnp allowed identification of structural features responsible for ligand affinities. | 2008 | 18855758 |
| listeria monocytogenes in voles. | | 1948 | 18880520 |
| listeria meningitis, a description of a strain of listeria monocytogenes and a report of a case. | | 1948 | 18884978 |
| unusual infections due to listeria monocytogenes in the southern california desert. | during the past 22 years, 14 patients have been hospitalized with infection due to listeria monocytogenes at the eisenhower medical center, a regional 300-bed hospital in the desert southwest of southern california. a large number of patients are retired, elderly, and have underlying and often systemic disease. | 2008 | 18922716 |
| short-term inhalation exposure to mild steel welding fume had no effect on lung inflammation and injury but did alter defense responses to bacteria in rats. | many workers worldwide are continually exposed to complex aerosols generated from welding processes. the objective was to assess the effect of inhalation exposure to mild steel (ms) welding fume on lung injury, inflammation, and defense responses. male sprague-dawley rats were exposed to ms fume at a concentration of 40 mg/m(3) x 3 h/day x 3 or 10 days using a robotic welding fume generator. controls were exposed to filtered air. to assess lung defense responses, a group of animals were intratra ... | 2009 | 18925477 |
| novel functions of type i interferons revealed by infection studies with listeria monocytogenes. | infection of cells and mice with listeria monocytogenes stimulates production of type i interferons (ifn). these in turn sensitise the listeria host to lethal sequelae of infection with these bacteria. here, we summarise recent findings on the production and biological effects of type i ifn in the course of l. monocytogenes infection. | 2008 | 18926303 |
| listerial rhombencephalitis in an immunocompetent young adult. | listeria monocytogenes is a common cause of central nervous system infections, especially in immunosuppressed patients, infants and elderly people. listerial rhombencephalitis is a rare and severe infection of the brainstem that is reported to have high mortality and frequent serious sequelae for survivors. we report the case of a 19-year-old healthy male who presented with listerial brainstem infection due to listeria monocytogenes. | 2009 | 18926754 |
| growth, survival, proliferation and pathogenesis of listeria monocytogenes under low oxygen or anaerobic conditions: a review. | listeria monocytogenes is a gram positive facultative anaerobe that causes listeriosis, a disease that mainly affects the immune-compromised, the elderly, infants and pregnant women. in the susceptible immune challenged population, listeriosis is very severe and has a fatality rate of up to 30%. control of l. monocytogenes is difficult due to its: 1) widespread presence in the environment, 2) intrinsic physiological resistance, 3) ability to adapt to external stresses and 4) ability to grow at a ... | 2009 | 18926916 |
| development of a quantitative polymerase chain reaction method using a live bacterium as internal control for the detection of listeria monocytogenes. | a novel real-time quantitative polymerase chain reaction (q-pcr) assay was developed for rapid and accurate detection of listeria monocytogenes. in this q-pcr assay, a computational dna random shuffling method was used to design an internal amplification control (iac) sequence, which was the same in length and g + c content to the hly amplicon. this iac sequence was inserted into the genome of l. monocytogenes to create a mutant strain named l. monocytogenes-iac. the lm-iac was used as an intern ... | 2008 | 18930623 |
| random mutagenesis identifies novel genes involved in the secretion of antimicrobial, cell wall-lytic enzymes by lactococcus lactis. | lactococcus lactis is a gram-positive bacterium that is widely used in the food industry and is therefore desirable as a candidate for the production and secretion of recombinant proteins. previously, we generated a l. lactis strain that expressed and secreted the antimicrobial cell wall-lytic enzyme lysostaphin. to identify lactococcal gene products that affect the production of lysostaphin, we isolated and characterized mutants generated by random transposon mutagenesis that had altered lysost ... | 2008 | 18931288 |
| multilocus genotyping assays for single nucleotide polymorphism-based subtyping of listeria monocytogenes isolates. | listeria monocytogenes is responsible for serious invasive illness associated with consumption of contaminated food and places a significant burden on public health and the agricultural economy. we recently developed a multilocus genotyping (mlgt) assay for high-throughput subtype determination of l. monocytogenes lineage i isolates based on interrogation of single nucleotide polymorphisms (snps) via multiplexed primer extension reactions. here we report the development and validation of two add ... | 2008 | 18931295 |
| pathogenicity of listeria monocytogenes scott a after oral and oculonasal challenges of day-old turkey poults. | listeria monocytogenes is a ubiquitous, environmental pathogen that has contaminated poultry ready-to-eat products resulting in large-scale recalls. research is needed to determine the source of product and processing plant contamination with l. monocytogenes. the purpose of this study was to compare the oral and oculonasal routes of infection on the pathogenicity of l. monocytogenes in turkey poults under different housing conditions. one-day-old turkey poults were challenged by either route wi ... | 2008 | 18939633 |
| prevalence of listeria monocytogenes in poultry production in france. | this study aimed to update and create a data set from laying hens and broilers regarding contamination by listeria monocytogenes. two hundred laying-hen flocks were sampled, with 88 flocks reared in cages and 112 reared on the floor. one hundred forty-five broiler flocks were sampled, with 85 conventional and 60 free-range flocks. a total of 774 and 725 samples were analyzed from laying hens and broilers, respectively. l. monocytogenes was detected in 31 of 200 flocks, yielding an estimated prev ... | 2008 | 18939743 |
| elimination of listeria monocytogenes from vacuum-packed dry-cured ham by e-beam radiation. | the inactivation kinetics for listeria monocytogenes scott a (cip 103575, serotype 4b) and listeria innocua (ntc 11288) after e-beam radiation were studied in vacuum-packed ready-to-eat dry-cured ham to optimize the sanitation treatment of this product. a treatment of 1.12 kgy was calculated to reach the food safety objective according to the u.s. department of agriculture criterion. no irradiation treatment is necessary to meet the european union microbiological criterion for this bacterium. no ... | 2008 | 18939744 |
| inactivation kinetics of three listeria monocytogenes strains under high hydrostatic pressure. | high hydrostatic pressure (hhp) inactivation of three listeria monocytogenes strains (egde, lo28, and scott a) subjected to 350 mpa at 20 degrees c in aces buffer resulted in survival curves with significant tailing for all three strains. a biphasic linear model could be fitted to the inactivation data, indicating the presence of an hhp-sensitive and an hhp-resistant fraction, which both showed inactivation according to first-order kinetics. inactivation parameters of these subpopulations of the ... | 2008 | 18939745 |
| survival of listeria monocytogenes in a simulated dynamic gastrointestinal model during storage of inoculated bologna and salami slices in vacuum packages. | listeria monocytogenes counts were determined during storage (82 days, 4 degrees c) in vacuum packages of inoculated bologna and salami slices and after exposure to a simulated dynamic model of the stomach and small intestine. variables controlled in the model included gastric emptying and gastrointestinal fluid secretion rates, gradual gastric acidification, and intestinal ph maintenance. l. monocytogenes populations increased on bologna and decreased on salami, reaching 8.7 and 1.4 log cfu/g, ... | 2008 | 18939746 |
| rechargeable antimicrobial surface modification of polyethylene. | polyethylene films were surface modified, to incorporate amine and amide functionalities, and subsequently were evaluated for their ability to recharge the antimicrobial n-halamine structures after contact with sodium hypochlorite, a common food-approved sanitizer. surfaces were tested for chlorine retention and release, as well as antimicrobial activity against microorganisms relevant to food quality and food safety, including escherichia coli k-12, pseudomonas fluorescens, bacillus cereus, and ... | 2008 | 18939750 |
| transmission electron microscopy study of listeria monocytogenes treated with nisin in combination with either grape seed or green tea extract. | the objective of this study was to use transmission electron microscopy to investigate the morphological changes that occurred in listeria monocytogenes cells treated with grape seed extract (gse), green tea extract (gte), nisin, and combinations of nisin with either gse or gte. the test solutions were prepared with (i) 1% gse, 1% gte, 6,400 iu of nisin, and the combination of these dilutions with nisin or with (ii) the pure major phenolic constituents of gse (0.02% epicatechin plus 0.02% catech ... | 2008 | 18939761 |
| comparison of four commercial dna extraction kits for pcr detection of listeria monocytogenes, salmonella, escherichia coli o157:h7, and staphylococcus aureus in fresh, minimally processed vegetables. | four commercial dna extraction methods, prepman ultra (applied biosystems), instagene matrix (biorad), dneasy tissue kit (qiagen), and ultraclean (mobio), were tested for pcr detection of listeria monocytogenes, escherichia coli o157: h7, salmonella, and staphylococcus aureus in fresh, minimally processed vegetables. for comparative purposes, sensitivity assays with specific pcrs were carried out after dna extraction with the four methods in green pepper, broccoli, and onion artificially inocula ... | 2008 | 18939762 |
| ox40 costimulatory signals potentiate the memory commitment of effector cd8+ t cells. | a t cell costimulatory molecule, ox40, contributes to t cell expansion, survival, and cytokine production. although several roles for ox40 in cd8(+) t cell responses to tumors and viral infection have been shown, the precise function of these signals in the generation of memory cd8(+) t cells remains to be elucidated. to address this, we examined the generation and maintenance of memory cd8(+) t cells during infection with listeria monocytogenes in the presence and absence of ox40 signaling. we ... | 2008 | 18941188 |
| exploring regulatory mechanisms of cd8+ t cell contraction. | a small fraction of the cd8 t cell effector population that responds to an infection progresses through the contraction phase to the memory stage. the factors regulating the extent of contraction are poorly understood. competition for limited resources has been widely postulated to be the cause of cell death during the contraction phase, but our data show that competition does not affect contraction kinetics. we go on to demonstrate that all effector cells present at the peak of the response hav ... | 2008 | 18946035 |
| antimicrobial activity of endemic crataegus tanacetifolia (lam.) pers and observation of the inhibition effect on bacterial cells. | up to now an increasing number of antibiotic-resistant bacteria have been reported and thus new natural therapeutic agents are needed in order to eradicate these pathogens. through the discovery of plants such as crataegus tanacetifolia (lam.) pers that have antimicrobial activity, it will be possible to discover new natural drugs serving as chemotherapeutic agents for the treatment of nosocomial pathogens and take these antibiotic-resistant bacteria under control. the objective of the present s ... | 2008 | 18946875 |
| adenovirus-mediated gene transfer of pathogen-associated molecular patterns for cancer immunotherapy. | the delivery of stimulatory signals to dendritic cells (dcs) in the tumor microenvironment could be an effective means to break tumor-induced tolerance. the work presented here evaluates the immunostimulatory properties of pathogen-associated molecular patterns (pamps), microbial molecules which bind toll-like receptors and deliver activating signals to immune cells, when expressed in tumor cells using adenoviral (ad) vectors. in vitro, transduction of a549 tumor cells with ad vectors expressing ... | 2009 | 18949016 |
| [listeria monocytogenes: old bug, permanent challenge]. | | 2008 | 18949141 |
| [listeria monocytogenes outbreaks: a review of the routes that favor bacterial presence]. | listeria monocytogenes is a foodborne pathogen that causes serious invasive illness, mainly in certain well-defined high-risk groups, including immunocompromised patients, pregnant women and neonates. l. monocytogenes primarily causes abortion, septicaemia or infections of the central nervous systems. listeriosis outbreaks have mostly been linked to consumption of raw milk or cheese made of unpasteurized milk. previous outbreaks of listeriosis have been linked to a variety of foods especially pr ... | 2008 | 18949142 |
| [listeria monocytogenes infection in pregnancy: experience of pontificia universidad católica de chile university hospital]. | listeria monocytogenes is gram-positive facultative intracellular pathogen often foodborne and found elsewhere. it is an uncommon cause of illness in the general population. however, it is an important cause of severe infection in neonates, pregnant women, elderly and immunosuppressed patients. listeriosis has unique preference for pregnant women. maternal listeriosis is a diagnostic challenge, and intrauterine infection can lead to severe complications such as amnionitis, preterm labor, spontan ... | 2008 | 18949143 |
| [listeria monocytogenes: report of a rise in pregnant women and literature review]. | listeria monocytogenes, rare pathogen in the general population, causes serious infections in patients at the extreme ages of life, pregnant woman, and those with immunosuppression. the clinical manifestations are essential to suspect the disease in patients at risk, allowing an early prescription of antimicrobial therapy, before the results of the cultures are available. clinical course and prognosis depends on how early treatment is started and, in pregnant women, the gestational age. in clíni ... | 2008 | 18949144 |
| [microbiological portrait: listeria monocytogenes]. | | 2008 | 18949145 |
| [historical note: in homage to lister]. | joseph lister, who disputes with klebs the third place as the father of microbiology, was just about to remain without a bacteria immortalizing his ñame. thanks to seeliger, now listeria is used for identifying the genus that pirie had already named listerella in 1927. through a quick review of the history of listeria monocytogenes, we get to know the principal facts in the lives of pirie and seeliger, investigators with absolutely different characters. we also review a brief history of the life ... | 2008 | 18949146 |
| relationship between sublethal injury and microbial inactivation by the combination of high hydrostatic pressure and citral or tert-butyl hydroquinone. | the aim was to investigate (i) the occurrence of sublethal injury in listeria monocytogenes, escherichia coli, and saccharomyces cerevisiae after high hydrostatic pressure (hhp) treatment as a function of the treatment medium ph and composition and (ii) the relationship between the occurrence of sublethal injury and the inactivating effect of a combination of hhp and two antimicrobial compounds, tert-butyl hydroquinone (tbhq) and citral. the three microorganisms showed a high proportion of suble ... | 2008 | 18952869 |
| functional impact of mutational activation on the listeria monocytogenes central virulence regulator prfa. | the transcriptional activator prfa is required for the expression of virulence factors necessary for listeria monocytogenes pathogenesis. prfa is believed to become activated following l. monocytogenes entry into the cytosol of infected host cells, resulting in the induction of target genes whose products are required for bacterial intracellular growth and cell-to-cell spread. several mutations have been identified that appear to lock prfa into its highly activated cytosolic form (known as prfa* ... | 2008 | 18957610 |
| chromium in stainless steel welding fume suppresses lung defense responses against bacterial infection in rats. | pulmonary infections have been reported to be increased in welders. previous animal studies have indicated that manual metal arc, stainless steel welding fume (mma-ss) increased susceptibility to lung infections. mma-ss is composed of a complex of metals (e.g., iron, chromium, nickel). the objective was to determine which metal component of mma-ss welding fume alters lung defense responses. at day 0, rats were intratracheally instilled one time with saline or mma-ss at a concentration of 2 mg/ra ... | 2007 | 18958720 |
| evaluation of enterococcus mundtii st4sa and lactobacillus plantarum 423 as probiotics by using a gastro-intestinal model with infant milk formulations as substrate. | enterococcus mundtii st4sa and lactobacillus plantarum 423 produce bacteriocins with activity against a number of gram-positive and gram-negative bacteria. both strains survived intestinal conditions simulated in a gastro-intestinal model (gim) with infant milk formulations as substrate and prevented the growth of listeria monocytogenes scotta. the strains are inhibited by the antibiotics amoxicillin, cefadroxil, roxithromycin and doxycycline, anti-inflammatory medicaments containing meloxicam, ... | 2008 | 18963159 |
| recombinant single chain antibodies in bioelectrochemical sensors. | recombinant antibodies provide an emerging strategy in the development of new immunosensors. in particular, single chain antibodies (scfvs) can be isolated and expressed in bacterial systems that also allow their in vitro manipulation at the gene level. in this work, we present for the first time results of single-chain phage displayed antibodies combined with amperometric detection and its application as an immunosensor. the scfv is immobilized on a carbon electrode and used to capture and quan ... | 2001 | 18968440 |
| simultaneous detection of escherichia coli o157:h7, salmonella spp. and listeria monocytogenes with an array-based immunosorbent assay using universal protein g-liposomal nanovesicles. | a novel universal reagent for immunoassays, protein g-liposomal nanovesicles has been developed and successfully used in an immunomagnetic bead sandwich assay for the detection of escherichia coli o157:h7 [c.-s. chen, a.j. baeumner, r.a. durst, talanta 67 (2005) 205]. to demonstrate the universal capability of protein g-liposomal nanovesicles, this reagent was used to develop an array-based immunosorbent assay for the simultaneous detection of e. coli o157:h7, salmonella, and listeria monocytoge ... | 2006 | 18970559 |
| cell biology: a listeria escape trick. | | 2008 | 18972010 |
| inhibition of listeria monocytogenes by oregano, cranberry and sodium lactate combination in broth and cooked ground beef systems and likely mode of action through proline metabolism. | listeria monocytogenes is a food safety challenge in various food systems including fresh and frozen meat and poultry. natural antimicrobials from plant sources in combination with salts of organic acids have the potential to control l. monocytogenes. in this research the efficiency of water soluble phenolic extracts of oregano and cranberry in combination with sodium lactate was evaluated for control of l .monocytogenes. in both broth and cooked meat studies, the results indicated that the comb ... | 2008 | 18973962 |
| listeriosis. | | 2008 | 18977860 |
| streptococcus mutans smu.623c codes for a functional, metal-dependent polysaccharide deacetylase that modulates interactions with salivary agglutinin. | the genome sequence of the oral pathogen streptococcus mutans predicts the presence of two putative polysaccharide deacetylases. the first, designated pgda in this paper, shows homology to the catalytic domains of peptidoglycan deacetylases from streptococcus pneumoniae and listeria monocytogenes, which are both thought to be involved in the bacterial defense mechanism against human mucosal lysozyme and are part of the cazy family 4 carbohydrate esterases. s. mutans cells in which the pgda gene ... | 2009 | 18978064 |
| first molluscan tnf-alpha homologue of the tnf superfamily in disk abalone: molecular characterization and expression analysis. | tumor necrosis factor alpha (tnf-alpha) is considered as a multifunctional immune modulator that plays an important role in the innate and adaptive immune systems in vertebrates. here, we described the characterization and expression analysis of the first tnf-alpha homologue in mollusk abalone, named as abtnf-alpha. it has 930-bp full length with a 717-bp open reading frame (orf), encoding 239 amino acids. the abtnf-alpha amino acid sequence shows the characteristic tnf family signature, n-termi ... | 2009 | 18984056 |
| listeria monocytogenes in gorgonzola: subtypes, diversity and persistence over time. | l. monocytogenes represents a primary concern in the production of gorgonzola, a protected designation of origin (pdo) italian blue-veined cheese produced only in the piedmont and lombardy regions. l. monocytogenes isolates (n=95) obtained from gorgonzola rinds, paste, and production/ripening environments were serotyped and then genotyped using pulsed field gel electrophoresis (pfge). the goal of this study was to investigate the variability of l. monocytogenes pfge-types across different pdo go ... | 2009 | 18990461 |
| met receptor tyrosine kinase degradation is altered in response to the leucine-rich repeat of the listeria invasion protein internalin b. | entry of the bacterial pathogen listeria monocytogenes into host epithelial cells is critical for infection and virulence. one major pathway for listeria entry involves binding of the bacterial protein internalin b to the host receptor tyrosine kinase met (hepatocyte growth factor receptor). activation of met and downstream signaling cascades is critical for listeria entry. internalin b is composed of several structural domains including an n-terminal leucine-rich repeat that is sufficient for b ... | 2009 | 18990695 |
| the poloxamer p85 is protective against listeria monocytogenes invasion. | listeria monocytogenes remains an important foodborne pathogen, and strategies designed to decrease the susceptibility of selected patient populations to foodborne pathogens are therefore desirable. our objective was to determine if the poloxamer p85 was protective against l. monocytogenes infection. caco-2 cells were treated with 0.1% (w/v) p85 and challenged with 10(7) l. monocytogenes egd for 1 hour. a standard gentamicin protection assay was performed to determine invasion differences betwee ... | 2008 | 18991538 |
| evaluation of a multiplex pcr system for simultaneous detection of salmonella spp., listeria monocytogenes, and escherichia coli o157:h7 in foods and in food subjected to freezing. | conventional culture methods were compared to a multiplex polymerase chain reaction (pcr) assay for simultaneous detection of salmonella spp., listeria monocytogenes, and escherichia coli o157:h7 from enrichment cultures of various types of artificially inoculated and naturally contaminated foods. the multiplex pcr assay was evaluated in 44 types of spiked food samples, including meat, produce, fish, and dairy products targeting genes specific for each pathogen for simultaneous detection. the se ... | 2009 | 18991547 |
| the role of l. monocytogenes serotype 4b gtca in gastrointestinal listeriosis in a/j mice. | serotype 4b strains of listeria monocytogenes have been responsible for most large outbreaks of listeriosis. in l. monocytogenes serotype 4b, gtca and glta have been implicated in serotype-specific glycosylation of the teichoic acid of the cell wall with galactose and glucose. in this study, we investigated the impact of mutations in glta (resulting in absence of glucose on teichoic acid) and gtca (resulting in absence of galactose, and markedly reduced glucose on teichoic acid) on virulence fol ... | 2009 | 18991548 |
| pathological and bacteriological studies of hydrosalpinx in buffaloes. | the objectives of the present study were to investigate the bacteria accompanying hydrosalpinx of the buffalo cow and investigate the correlation between bacterial infection of the uterus and hydrosalpinx. buffalo cows' reproductive tracts were collected from mosul abattoir. a total 385 uterine samples were examined of which 25 were having hydrosalpinx. swabs for bacteriology, fluid for cytology and biopsies for histopathology were collected from the hydrosalpinx and the uterus from each samples ... | 2010 | 18992119 |
| the ability of listeria monocytogenes pi-plc to facilitate escape from the macrophage phagosome is dependent on host pkcbeta. | listeria monocytogenes are facultative intracellular pathogenic bacteria that can infect macrophages as well as non-professional phagocytes. after entry in the host cell, the bacteria escape from the phagosome into the cytoplasm. in murine macrophages and in cell lines derived from these cells, escape of l. monocytogenes from the phagosome is absolutely dependent on listeriolysin o (llo) and facilitated by a secreted phosphatidylinositol-specific phospholipase c (pi-plc). work in this laboratory ... | 2009 | 18996181 |