| psamm: a portable system for the analysis of metabolic models. | the genome-scale models of metabolic networks have been broadly applied in phenotype prediction, evolutionary reconstruction, community functional analysis, and metabolic engineering. despite the development of tools that support individual steps along the modeling procedure, it is still difficult to associate mathematical simulation results with the annotation and biological interpretation of metabolic models. in order to solve this problem, here we developed a portable system for the analysis ... | 2016 | 26828591 |
| identification of a conserved 5'-drp lyase activity in bacterial dna repair ligase d and its potential role in base excision repair. | bacillus subtilis is one of the bacterial members provided with a nonhomologous end joining (nhej) system constituted by the dna-binding ku homodimer that recruits the atp-dependent dna ligase d (bsuligd) to the double-stranded dna breaks (dsbs) ends. bsuligd has inherent polymerization and ligase activities that allow it to fill the short gaps that can arise after realignment of the broken ends and to seal the resulting nicks, contributing to genome stability during the stationary phase and ger ... | 2016 | 26826709 |
| hexr controls glucose-responsive genes and central carbon metabolism in neisseria meningitidis. | neisseria meningitidis, an exclusively human pathogen and the leading cause of bacterial meningitis, must adapt to different host niches during human infection. n. meningitidis can utilize a restricted range of carbon sources, including lactate, glucose, and pyruvate, whose concentrations vary in host niches. microarray analysis of n. meningitidis grown in a chemically defined medium in the presence or absence of glucose allowed us to identify genes regulated by carbon source availability. most ... | 2016 | 26644430 |
| characterization of a carbapenem-hydrolyzing enzyme, poxb, in pseudomonas aeruginosa pao1. | pseudomonas aeruginosa is an opportunistic pathogen often associated with severe and life-threatening infections that are highly impervious to treatment. this microbe readily exhibits intrinsic and acquired resistance to varied antimicrobial drugs. resistance to penicillin-like compounds is commonplace and provided by the chromosomal ampc β-lactamase. a second, chromosomally encoded β-lactamase, poxb, has previously been reported in p. aeruginosa. in the present work, the contribution of this cl ... | 2016 | 26621621 |
| a hospital-wide outbreak of serratia marcescens, and ishikawa's "fishbone" analysis to support outbreak control. | a nosocomial outbreak of serratia marcescens in respiratory samples predominantly from patients in a surgical intensive care unit is reported. most of these patients were cardiac surgical patients. initially, a vigorous but inconclusive investigation was implemented on the basis of standardized (according the us centers for disease control and prevention) steps of outbreak investigation. then, a systemic quality management approach with "fishbone" analysis was added. as a consequence, plausible ... | 2016 | 26783861 |
| epidemiologic and genotypic review of carbapenemase-producing organisms in british columbia, canada, between 2008 and 2014. | carbapenemase-producing organisms (cpos) are a serious emerging problem for health care facilities worldwide. owing to their resistance to most antimicrobial therapies, cpos are difficult to treat and pose a challenge for infection prevention and control. since 2010, lab-based surveillance for cpos and pcr-based testing were implemented in british columbia (bc), canada. a review of cpos in bc from 2008 to march 2014 was done to characterize the resistance mechanisms and possible clonal strain tr ... | 2016 | 26607987 |
| specific gene loci of clinical pseudomonas putida isolates. | pseudomonas putida are ubiquitous inhabitants of soils and clinical isolates of this species have been seldom described. clinical isolates show significant variability in their ability to cause damage to hosts because some of them are able to modulate the host's immune response. in the current study, comparisons between the genomes of different clinical and environmental strains of p. putida were done to identify genetic clusters shared by clinical isolates that are not present in environmental ... | 2016 | 26820467 |
| a multi-substrate approach for functional metagenomics-based screening for (hemi)cellulases in two wheat straw-degrading microbial consortia unveils novel thermoalkaliphilic enzymes. | functional metagenomics is a promising strategy for the exploration of the biocatalytic potential of microbiomes in order to uncover novel enzymes for industrial processes (e.g. biorefining or bleaching pulp). most current methodologies used to screen for enzymes involved in plant biomass degradation are based on the use of single substrates. moreover, highly diverse environments are used as metagenomic sources. however, such methods suffer from low hit rates of positive clones and hence the dis ... | 2016 | 26822785 |
| threonine aldolases: perspectives in engineering and screening the enzymes with enhanced substrate and stereo specificities. | threonine aldolases have emerged as a powerful tool for asymmetric carbon-carbon bond formation. these enzymes catalyse the unnatural aldol condensation of different aldehydes and glycine to produce highly valuable β-hydroxy-α-amino acids with complete stereocontrol at the α-carbon and moderate specificity at the β-carbon. a range of microbial threonine aldolases has been recently recombinantly produced by several groups and their biochemical properties were characterized. numerous studies have ... | 2016 | 26810201 |
| pqsbc, a condensing enzyme in the biosynthesis of the pseudomonas aeruginosa quinolone signal: crystal structure, inhibition, and reaction mechanism. | pseudomonas aeruginosaproduces a number of alkylquinolone-type secondary metabolites best known for their antimicrobial effects and involvement in cell-cell communication. in the alkylquinolone biosynthetic pathway, the β-ketoacyl-(acyl carrier protein) synthase iii (fabh)-like enzyme pqsbc catalyzes the condensation of octanoyl-coenzyme a and 2-aminobenzoylacetate (2-aba) to form the signal molecule 2-heptyl-4(1h)-quinolone. pqsbc, a potential drug target, is unique for its heterodimeric arrang ... | 2016 | 26811339 |
| data on the standardization of a cyclohexanone-responsive expression system for gram-negative bacteria. | engineering of robust microbial cell factories requires the use of dedicated genetic tools somewhat different from those traditionally used for laboratory-adapted microorganisms. we have edited and formatted the chnr/p chnb regulatory node of acinetobacter johnsonii to ease the targeted engineering of ectopic gene expression in gram-negative bacteria. the proposed compositional standard was thoroughly verified with a monomeric and superfolder green fluorescent protein (msf•gfp) in escherichia co ... | 2016 | 26870759 |
| terpene arms race in the seiridium cardinale - cupressus sempervirens pathosystem. | the canker-causing fungus seiridium cardinale is the major threat to cupressus sempervirens worldwide. we investigated the production of terpenes by canker-resistant and susceptible cypresses inoculated with s. cardinale, the effect of these terpenes on fungal growth, and the defensive biotransformation of the terpenes conducted by the fungus. all infected trees produced de novo terpenes and strongly induced terpenic responses, but the responses were stronger in the canker-resistant than the sus ... | 2016 | 26796122 |
| mvat family proteins encoded on incp-7 plasmid pcar1 and the host chromosome regulate the host transcriptome cooperatively but differently. | mvat proteins are members of the h-ns family of proteins in pseudomonads. the incp-7 conjugative plasmid pcar1 carries an mvat-homologous gene, pmr. in pseudomonas putida kt2440 bearing pcar1, pmr and the chromosomally carried homologous genes, tura and turb, are transcribed at high levels, and pmr interacts with tura and turb in vitro. in the present study, we clarified how the three mvat proteins regulate the transcriptome of p. putida kt2440(pcar1). analyses performed by a modified chromatin ... | 2016 | 26590283 |
| isolation of polyvalent bacteriophages by sequential multiple-host approaches. | many studies on phage biology are based on isolation methods that may inadvertently select for narrow-host-range phages. consequently, broad-host-range phages, whose ecological significance is largely unexplored, are consistently overlooked. to enhance research on such polyvalent phages, we developed two sequential multihost isolation methods and tested both culture-dependent and culture-independent phage libraries for broad infectivity. lytic phages isolated from activated sludge were capable o ... | 2016 | 26590277 |
| bromochloromethane, a methane analogue, affects the microbiota and metabolic profiles of the rat gastrointestinal tract. | bromochloromethane (bcm), an inhibitor of methanogenesis, has been used in animal production. however, little is known about its impact on the intestinal microbiota and metabolic patterns. the present study aimed to investigate the effect of bcm on the colonic bacterial community and metabolism by establishing a wistar rat model. twenty male wistar rats were randomly divided into two groups (control and treated with bcm) and raised for 6 weeks. bacterial fermentation products in the cecum were d ... | 2016 | 26567308 |
| substrate and cofactor range differences of two cysteine dioxygenases from ralstonia eutropha h16. | cysteine dioxygenases (cdos), which catalyze the sulfoxidation of cysteine to cysteine sulfinic acid (csa), have been extensively studied in eukaryotes because of their roles in several diseases. in contrast, only a few prokaryotic enzymes of this type have been investigated. in ralstonia eutropha h16, two cdo homologues (cdoa and cdob) have been identified previously. in vivo studies showed that escherichia coli cells expressing cdoa could convert 3-mercaptopropionate (3mp) to 3-sulfinopropiona ... | 2016 | 26590284 |
| cd(ii) sorption on montmorillonite-humic acid-bacteria composites. | soil components (e.g., clays, bacteria and humic substances) are known to produce mineral-organic composites in natural systems. herein, batch sorption isotherms, isothermal titration calorimetry (itc), and cd k-edge exafs spectroscopy were applied to investigate the binding characteristics of cd on montmorillonite(mont)-humic acid(ha)-bacteria composites. additive sorption and non-additive cd(ii) sorption behaviour is observed for the binary mont-bacteria and ternary mont-ha-bacteria composite, ... | 2016 | 26792640 |
| engineering cell factories for producing building block chemicals for bio-polymer synthesis. | synthetic polymers are widely used in daily life. due to increasing environmental concerns related to global warming and the depletion of oil reserves, the development of microbial-based fermentation processes for the production of polymer building block chemicals from renewable resources is desirable to replace current petroleum-based methods. to this end, strains that efficiently produce the target chemicals at high yields and productivity are needed. recent advances in metabolic engineering h ... | 2016 | 26794242 |
| highly active and stable large catalase isolated from a hydrocarbon degrading aspergillus terreus mtcc 6324. | a hydrocarbon degrading aspergillus terreus mtcc 6324 produces a high level of extremely active and stable cellular large catalase (cat) during growth on n-hexadecane to combat the oxidative stress caused by the hydrocarbon degrading metabolic machinery inside the cell. a 160-fold purification with specific activity of around 66 × 10(5) u mg(-1) protein was achieved. the native protein molecular mass was 368 ± 5 kda with subunit molecular mass of nearly 90 kda, which indicates that the native ca ... | 2016 | 27057351 |
| uncovering the transmembrane metal binding site of the novel bacterial major facilitator superfamily-type copper importer ccoa. | uptake and trafficking of metals and their delivery to their respective metalloproteins are important processes. cells need precise control of each step to avoid exposure to excessive metal concentrations and their harmful consequences. copper (cu) is a required micronutrient used as a cofactor in proteins. however, in large amounts, it can induce oxidative damage; hence, cu homeostasis is indispensable for cell survival. biogenesis of respiratory heme-cu oxygen (hco) reductases includes inserti ... | 2016 | 26787831 |
| comparative genomics between two xenorhabdus bovienii strains highlights differential evolutionary scenarios within an entomopathogenic bacterial species. | bacteria of the genus xenorhabdus are symbionts of soil entomopathogenic nematodes of the genus steinernema. this symbiotic association constitutes an insecticidal complex active against a wide range of insect pests. within xenorhabdus bovienii species, the x. bovienii cs03 strain (xb cs03) is nonvirulent when directly injected into lepidopteran insects, and displays a low virulence when associated with its steinernema symbiont. the genome of xb cs03 was sequenced and compared with the genome of ... | 2016 | 26769959 |
| a loop-mediated isothermal amplification assay and sample preparation procedure for sensitive detection of xanthomonas fragariae in strawberry. | xanthomonas fragariae is a bacterium that causes angular leaf spot of strawberry. asymptomatic infection is common and contributes to the difficulties in disease management. the aim of this study was to develop a loop-mediated isothermal amplification (lamp) assay as an efficient method for detection of asymptomatic infections of x. fragariae. in addition, a new method of sample preparation was developed that allows sampling of a larger amount of plant tissue, hence increasing the detection rate ... | 2016 | 26766068 |
| bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness. | recent studies have shown that rates of bacterial dispersion in soils are controlled by hydration conditions that define size and connectivity of the retained aqueous phase. despite the ecological implications of such constraints, microscale observations of this phenomenon remain scarce. here, we quantified aqueous film characteristics and bacterial flagellated motility in response to systematic variations in microhydrological conditions on porous ceramic surfaces that mimic unsaturated soils. w ... | 2016 | 26757676 |
| construction of a subunit-fusion nitrile hydratase and discovery of an innovative metal ion transfer pattern. | metallochaperones are metal-binding proteins designed to deliver the appropriate metal to a target protein. the metal is usually transferred between different proteins. in this study, we discovered that metal was transferred between the same subunit of a mutant nitrile hydratase (nhase). various "activator proteins" mediate the trafficking of metal ions into nhases. we constructed fusion nhases by fusing the β- and α-subunits and/or the "activator proteins" of the nhase from pseudomonas putida. ... | 2016 | 26755342 |
| lignin engineering in field-grown poplar trees affects the endosphere bacterial microbiome. | cinnamoyl-coa reductase (ccr), an enzyme central to the lignin biosynthetic pathway, represents a promising biotechnological target to reduce lignin levels and to improve the commercial viability of lignocellulosic biomass. however, silencing of the ccr gene results in considerable flux changes of the general and monolignol-specific lignin pathways, ultimately leading to the accumulation of various extractable phenolic compounds in the xylem. here, we evaluated host genotype-dependent effects of ... | 2016 | 26755604 |
| the effect of root exudate 7,4'-dihydroxyflavone and naringenin on soil bacterial community structure. | our goal was to investigate how root exudate flavonoids influence the soil bacterial community structure and to identify members of the community that change their relative abundance in response to flavonoid exudation. using a model system that approximates flavonoid exudation of medicago sativa roots, we treated a soil with 7,4'-dihydroxyflavone and naringenin in two separate experiments using three different rates: medium (equivalent to the exudation rate of 7,4'-dihydroxyflavone from m. sativ ... | 2016 | 26752410 |
| going beyond the control of quorum-sensing to combat biofilm infections. | most bacteria attach to surfaces where they form a biofilm, cells embedded in a complex matrix of polymers. cells in biofilms are much better protected against noxious agents than free-living cells. as a consequence it is very difficult to control pathogens with antibiotics in biofilm infections and novel targets are urgently needed. one approach aims at the communication between cells to form and to maintain a biofilm, a process called quorum-sensing. water soluble small-sized molecules mediate ... | 2016 | 27025518 |
| diversity and interactions of microbial functional genes under differing environmental conditions: insights from a membrane bioreactor and an oxidation ditch. | the effect of environmental conditions on the diversity and interactions of microbial communities has caused tremendous interest in microbial ecology. here, we found that with identical influents but differing operational parameters (mainly mixed liquor suspended solid (mlss) concentrations, solid retention time (srt) and dissolved oxygen (do) concentrations), two full-scale municipal wastewater treatment systems applying oxidation ditch (od) and membrane bioreactor (mbr) processes harbored a ma ... | 2016 | 26743465 |
| identification of genes conferring tolerance to lignocellulose-derived inhibitors by functional selections in soil metagenomes. | the production of fuels or chemicals from lignocellulose currently requires thermochemical pretreatment to release fermentable sugars. these harsh conditions also generate numerous small-molecule inhibitors of microbial growth and fermentation, limiting production. we applied small-insert functional metagenomic selections to discover genes that confer microbial tolerance to these inhibitors, identifying both individual genes and general biological processes associated with tolerance to multiple ... | 2016 | 26546427 |
| characterization of the vibrio fischeri fatty acid chemoreceptors, vfcb and vfcb2. | bacteria use a wide variety of methyl-accepting chemotaxis proteins (mcps) to mediate their attraction to or repulsion from different chemical signals in their environment. the bioluminescent marine bacterium vibrio fischeri is the monospecific symbiont of the hawaiian bobtail squid, euprymna scolopes, and encodes a large repertoire of mcps that are hypothesized to be used during different parts of its complex, multistage lifestyle. here, we report the initial characterization of two such mcps f ... | 2016 | 26567312 |
| understanding butanol tolerance and assimilation in pseudomonas putida bird-1: an integrated omics approach. | pseudomonas putida bird-1 has the potential to be used for the industrial production of butanol due to its solvent tolerance and ability to metabolize low-cost compounds. however, the strain has two major limitations: it assimilates butanol as sole carbon source and butanol concentrations above 1% (v/v) are toxic. with the aim of facilitating bird-1 strain design for industrial use, a genome-wide mini-tn5 transposon mutant library was screened for clones exhibiting increased butanol sensitivity ... | 2016 | 26986205 |
| diffusion of bacterial cells in porous media. | the chemotaxis signal transduction network regulates the biased random walk of many bacteria in favorable directions and away from harmful ones through modulating the frequency of directional reorientations. in mutants of diverse bacteria lacking the chemotaxis response, migration in classic motility agar, which constitutes a fluid-filled porous medium, is compromised; straight-swimming cells unable to tumble become trapped within the agar matrix. spontaneous mutations that restore spreading hav ... | 2016 | 26745427 |
| efficient and automated large-scale detection of structural relationships in proteins with a flexible aligner. | the total number of known three-dimensional protein structures is rapidly increasing. consequently, the need for fast structural search against complete databases without a significant loss of accuracy is increasingly demanding. recently, topsearch, an ultra-fast method for finding rigid structural relationships between a query structure and the complete protein data bank (pdb), at the multi-chain level, has been released. however, comparable accurate flexible structural aligners to perform effi ... | 2016 | 26732380 |
| the cytochrome p450 cyp6p4 is responsible for the high pyrethroid resistance in knockdown resistance-free anopheles arabiensis. | pyrethroid insecticides are the front line vector control tools used in bed nets to reduce malaria transmission and its burden. however, resistance in major vectors such as anopheles arabiensis is posing a serious challenge to the success of malaria control. herein, we elucidated the molecular and biochemical basis of pyrethroid resistance in a knockdown resistance-free anopheles arabiensis population from chad, central africa. using heterologous expression of p450s in escherichia coli coupled w ... | 2016 | 26548743 |
| the antibacterial activity and mechanism of ginkgolic acid c15:1. | the present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (ga) c15:1 monomer using green fluorescent protein (gfp)-labeled bacteria strains. | 2017 | 28088196 |
| metabolic pathway for a new strain pseudomonas synxantha lsh-7': from chemotaxis to uptake of n-hexadecane. | bacteria can use n-hexadecane as a carbon source, but it remains incompletely understood whether n-hexadecane is transformed into metabolic intermediates prior to cellular uptake or not. we newly isolated a strain identified as pseudomonas synxantha lsh-7' and conducted chemotaxis experiment of this bacterial strain towards n-hexadecane, hexadecanol and hexadecanoic acid with qualitative assays respectively. furthermore, we described the identification of extracellular alkane hydroxylase and alc ... | 2017 | 28051099 |
| enhanced heterologous protein productivity by genome reduction in lactococcus lactis nz9000. | the implementation of novel chassis organisms to be used as microbial cell factories in industrial applications is an intensive research field. lactococcus lactis, which is one of the most extensively studied model organisms, exhibits superior ability to be used as engineered host for fermentation of desirable products. however, few studies have reported about genome reduction of l. lactis as a clean background for functional genomic studies and a model chassis for desirable product fermentation ... | 2017 | 28049473 |
| isolation and identification of phyllospheric bacteria possessing antimicrobial activity from astragalus obtusifolius, prosopis juliflora, xanthium strumarium and hippocrepis unisiliqousa. | the widespread utilization of antimicrobial compounds has caused emergence of resistant microorganisms in the world. hence, the research to probe the products with antimicrobial features has led to finding natural habitats and discovering new pharmaceutical products. | 2017 | 28090278 |
| heavy metal tolerance genes alter cellular thermodynamics in pseudomonas putida and river pseudomonas spp. and influence amebal predation. | predation rates were measured for two acanthamoeba castellanii strains feeding on metal-tolerant and metal-sensitive strains of pseudomonas putida and compared with cellular thermodynamic data. predation rates by a. castellanii strain atcc 30010 correlated with cell volume of the prey. to explore whether this observation could be environmentally relevant, pseudomonad species were isolated from a pristine and a metal-contaminated river and were paired based on phylogenetic and physiological relat ... | 2013 | 23895438 |
| enhanced bacterial metabolism of a pseudomonas strain in response to the addition of culture filtrate of a bacteriophagous amoeba. | in a previous work, levrat et al. [21] showed an enhancement of the production of pyoverdin (siderophore) by pseudomonas putida in the presence of amoeba. to explain the mechanism of stimulation, the hypothesis of production of stimulatory factors by amoeba was proposed. filtrates of both mixed culture of bacteria and amoeba (pseudomonas putida + acanthamoeba castellanii) and of axenic culture of amoeba were added to the culture medium of pseudomonas. the production of pyoverdin was increased in ... | 1992 | 23194985 |
| regio- and stereoselective aliphatic-aromatic cross-benzoin reaction: enzymatic divergent catalysis. | the catalytic asymmetric synthesis of chiral 2-hydroxy ketones by using different thiamine diphosphate dependent enzymes, namely benzaldehyde lyase from pseudomonas fluorescens (pfbal), a variant of benzoylformate decarboxylase from pseudomonas putida (ppbfd-l461a), branched-chain 2-keto acid decarboxylase from lactococcus lactis (llkdca) and a variant of pyruvate decarboxylase from acetobacter pasteurianus (appdc-e469g), was studied. starting with the same set of substrates, substituted benzald ... | 2016 | 27515897 |
| sequencing of plasmids pambl1 and pambl2 from pseudomonas aeruginosa reveals a blavim-1 amplification causing high-level carbapenem resistance. | carbapenemases are a major concern for the treatment of infectious diseases caused by gram-negative bacteria. although plasmids are responsible for the spread of resistance genes among these pathogens, there is limited information on the nature of the mobile genetic elements carrying carbapenemases in pseudomonas aeruginosa. | 2015 | 26209313 |
| development of a growth-dependent selection system for identification of l-threonine aldolases. | threonine aldolases (tas) are useful enzymes for the synthesis of β-hydroxy-α-amino acids due to their capability to catalyze asymmetric aldol reactions. starting from two prochiral compounds, an aldehyde and glycine, two chiral stereocenters were formed in a single step via c-c bond formation. owing to poor diastereoselectivity and low activity, the enzymatic synthesis of β-hydroxy-α-amino acids by tas is still a challenge. for identification of new tas, a growth-dependent selection system in p ... | 2015 | 25616526 |
| the influence of bioaugmentation and biosurfactant addition on bioremediation efficiency of diesel-oil contaminated soil: feasibility during field studies. | the study focused on assessing the influence of bioaugmentation and addition of rhamnolipids on diesel oil biodegradation efficiency during field studies. initial laboratory studies (measurement of emitted co2 and dehydrogenase activity) were carried out in order to select the consortium for bioaugmentation as well as to evaluate the most appropriate concentration of rhamnolipids. the selected consortium consisted of following bacterial taxa: aeromonas hydrophila, alcaligenes xylosoxidans, gordo ... | 2014 | 24291585 |
| prevalence and antimicrobial resistance pattern of bacterial strains isolated from patients with urinary tract infection in messalata central hospital, libya. | to investigate the prevalence of urinary tract infection among patients at messalata central hospital, libya, to identify the causative bacteria, and to explore their resistance pattern to antimicrobials. | 2016 | 27569886 |
| application of cinder gel-beads/reeds combination strategy for bioremediation of pyrene- and indeno(1,2,3-cd)pyrene-contaminated estuarine wetlands. | pseudomonas putida pyr1 and acinetobacter baumannii inp1 isolated from liaohe estuarine wetlands were entrapped in cinder beads to make cinder gel-beads. they were combined with reeds for bioremediation of pyrene- and indeno(1,2,3-cd)pyrene-contaminated estuarine wetlands. the results showed that the removal percentages of pyrene and indeno(1,2,3-cd)pyrene (69.2 and 89.8 % respectively) in 40 days using cinder gel-beads/reeds were obviously higher than those using cinder gel-beads(52.6 and 70.0 ... | 2016 | 26897584 |
| [oil biodegradation by microbial-plant associations]. | the degradation of petroleum hydrocarbons by plant-microbial associations, as well as the peculiarities of the interaction between microorganisms in consortium and the associated plants, have been studied. it was shown that degrader microorganisms that are part of the consortium rhodococcus erythropolis s26, acinetobacter baumannii 1 b, acinetobacter baumannii 7, and pseudomonas putida f701 were effective in the degradation of oil and were good colonizers of plant roots (barley). the efficiency ... | 2015 | 26027354 |
| proteomic characterization of the outer membrane vesicle of pseudomonas putida kt2440. | outer membrane vesicles (omvs) are produced by various pathogenic gram-negative bacteria such as escherichia coli, pseudomonas aeruginosa, and acinetobacter baumannii. in this study, we isolated omvs from a representative soil bacterium, pseudomonas putida kt2440, which has a biodegradative activity toward various aromatic compounds. proteomic analysis identified the outer membrane proteins (omps) oprc, oprd, opre, oprf, oprh, oprg, and oprw as major components of the omv of p. putida kt2440. th ... | 2014 | 25198519 |
| [investigation of the presence of new delhi metallo-beta-lactamase-1 (ndm-1) by pcr in carbapenem-resistant gram-negative isolates]. | bacteria producing new delhi metallo-beta-lactamase-1 (ndm-1) exhibit high level resistance to beta-lactams including carbapenems. this broad-spectrum resistance limits treatment options for infections caused by ndm-1 producers. ndm-1 was first isolated from an indian patient in sweden; since then, ndm-1 producing isolates have been identified in many countries including turkey. in this study, we investigated the presence of ndm-1 by pcr method in various gram-negative isolates recovered from cl ... | 2013 | 23621739 |
| identification and characterization of an indigo-producing oxygenase involved in indole 3-acetic acid utilization by acinetobacter baumannii. | acinetobacter baumannii harbours a gene cluster similar to the iac locus of pseudomonas putida 1290, which can catabolize the plant hormone indole 3-acetic acid (iaa) as an energy source. however, there has been no evidence showing that iaa can be utilized by a. baumannii. this study showed that a. baumannii can grow in m9 minimal medium containing iaa as the sole carbon source. a mutagenesis study indicated that iaca, encoded in the iac locus of a. baumannii, is involved in the catabolism of ia ... | 2012 | 22311185 |
| efficacy of lactoferricin b in controlling ready-to-eat vegetable spoilage caused by pseudomonas spp. | the microbial content of plant tissues has been reported to cause the spoilage of ca. 30% of chlorine-disinfected fresh vegetables during cold storage. the aim of this work was to evaluate the efficacy of antimicrobial peptides in controlling microbial vegetable spoilage under cold storage conditions. a total of 48 bacterial isolates were collected from ready-to-eat (rte) vegetables and identified as belonging to acinetobacter calcoaceticus, aeromonas media, pseudomonas cichorii, pseudomonas flu ... | 2015 | 26453993 |
| appraising freeze-drying for storage of bacteria and their ready access in a rapid toxicity assessment assay. | direct toxicity assessment (dta) techniques seek to measure the impact of toxic chemicals on biological materials resident in the environment. this study features the use of freeze-dried bacterial cells in combination with a rapid dta analyser, scitox. the effects of three factors-cryoprotectant type, bacterial strain, and storage temperature-were tested in order to validate the shelf life of the freeze-dried cells. three freeze-dried gram-negative bacterial strains, acinetobacter calcoaceticus, ... | 2013 | 23377787 |
| unusual microorganisms and antimicrobial resistances in a group of syrian migrants: sentinel surveillance data from an asylum seekers centre in italy. | three years of civil war in syria have caused death and increase of communicable diseases. the suffering population has been forced to migrate creating a fertile condition for epidemic spread of infection within the refugee camps. | 2017 | 26987764 |
| degradation capacities of bacteria and yeasts isolated from the gut of dendroctonus rhizophagus (curculionidae: scolytinae). | bark beetles (curculionidae: scolytinae) feed on the xylem and phloem of their host, which are composed of structural carbohydrates and organic compounds that are not easily degraded by the insects. some of these compounds might be hydrolyzed by digestive enzymes produced by microbes present in the gut of these insects. in this study, we evaluated the enzymatic capacity of bacteria (acinetobacter lwoffii, arthrobacter sp., pseudomonas putida, pseudomonas azotoformans, and rahnella sp.) and yeast ... | 2017 | 27544667 |
| in vitro evidence of chain transfer to tetraethylene glycols in enzymatic polymerization of polyhydroxyalkanoate. | a polyhydroxyalkanoate (pha) was enzymatically synthesized in vitro, and the end structure of pha associated with a chain transfer (ct) reaction was investigated. in the ct reaction, pha chain transfers from pha synthase (phac) to a ct agent, resulting in covalent bonding of ct agent to the pha chain at its carboxyl end. in vitro ct reaction has never been demonstrated because of relatively low yields of in vitro synthesized poly[(r)-3-hydroxybutyrate)] (p(3hb)), which makes it difficult to char ... | 2013 | 23474615 |
| silver nanoparticles formation by extracellular polymeric substances (eps) from electroactive bacteria. | microbial extracellular polymeric substances (eps) excreted from microorganisms were a complex natural biological polymer mixture of proteins and polysaccharides, which played an important roles in the transport of metals, such as ag(+). electroactive bacteria, is an important class of environmental microorganisms, which can use iron or manganese mineral as terminal electron acceptors to generate energy for biosynthesis and cell maintenance. in this work, the eps extracted of three electroactive ... | 2016 | 26797954 |
| bacterial study of fin rot in brown trout by api20e. | in order to study caudal fin rot with emphasis on aeromonas sp. and pseudomonas sp. in salmo trutta caspius from the salmonids propagation and breeding center of shahid bahonar of kelardasht region, one hundred and eighty brood stocks having fin damage symptoms were chosen. two bacterial samples from each fish were cultured on aeromonas and pseudomonas specific media. to identify isolated bacterial colonies by api20e diagnostic system, samples obtained from bacterial cultures 18 to 24 h were pre ... | 2014 | 24897801 |
| microorganisms hydrolyse amide bonds; knowledge enabling read-across of biodegradability of fatty acid amides. | to get insight in the biodegradation and potential read-across of fatty acid amides, n-[3-(dimethylamino)propyl] cocoamide and n-(1-ethylpiperazine) tall oil amide were used as model compounds. two bacteria, pseudomonas aeruginosa pk1 and pseudomonas putida pk2 were isolated with n-[3-(dimethylamino)propyl] cocoamide and its hydrolysis product n,n-dimethyl-1,3-propanediamine, respectively. in mixed culture, both strains accomplished complete mineralization of n-[3-(dimethylamino)propyl] cocoamid ... | 2014 | 24509885 |
| spalf4: a newly identified anti-lipopolysaccharide factor from the mud crab scylla paramamosain with broad spectrum antimicrobial activity. | anti-lipopolysaccharide factors (alfs) are antimicrobial peptides with binding and neutralizing activities to lipopolysaccharide (lps) in crustaceans. this study identified and characterized a novel alf homolog (spalf4) from the mud crab scylla paramamosain. the complete cdna of spalf4 had 756 bp with a 381 bp open reading frame encoding a protein with 126 aa. the deduced protein contained a signal peptide and a lps-binding domain. spalf4 shared the highest identity with ptalf5 at amino acid lev ... | 2014 | 24239582 |
| pseudomonas putida strain fstm2 isolated from shark skin: a potential source of bacteriocin. | bacteriocin-producing pseudomonas putida strain fstm2 isolated from shark showed broad range of antibacterial activity against all pathogens tested except bacillus subtilis atcc11774, mrsa n32064, proteus mirabilis atcc12453, enterococcus faecalis atcc14506, salmonella typhimurium atcc51312, salmonella mutan atcc25175, and aeromonas hydrophila wbf314. of the three growth media tested in this study, tsb was observed to support the bacteriocin activity the most. while the highest bacteriocin activ ... | 2013 | 26782985 |
| engineered escherichia coli for short-chain-length medium-chain-length polyhydroxyalkanoate copolymer biosynthesis from glycerol and dodecanoate. | short-chain-length medium-chain-length polyhydroxyalkanoate (scl-mcl pha) copolymers are promising as bio-plastics with properties ranging from thermoplastics to elastomers. in this study, the hybrid pathway for the biosynthesis of scl-mcl pha copolymers was established in recombinant escherichia coli by co-expression of β-ketothiolase (phaare) and nadph-dependent acetoacetyl-coa reductase (phabre) from ralstonia eutropha together with pha synthases from r. eutropha (phacre), aeromonas hydrophil ... | 2013 | 23748789 |
| broad diversity of conjugative plasmids in integron-carrying bacteria from wastewater environments. | in this study we assessed the occurrence, diversity and conjugative potential of plasmids in integron-carrying aeromonas and enterobacteriaceae from wastewaters. sixty-six strains were included as donors in mating assays using rifampicin-resistant escherichia coli and pseudomonas putida recipient strains. the diversity of plasmids from donors and transconjugants (resistant to tetracycline or streptomycin) was evaluated by restriction analysis and replicon typing targeting 19 incompatibility grou ... | 2012 | 22409355 |
| growth ability of gram negative bacteria in free-living amoebae. | when bacteria and free-living amoebae (flas) live both in natural waters and man-made aquatic systems, they constantly interact with each other. some bacteria can survive and grow within flas. therefore, it has recently been thought that flas play an important role in spreading pathogenic bacteria in aquatic systems. in this study we investigated the intracellular growing ability of 7 different gram-negative bacteria (pseudomonas fluorescens, pseudomonas putida, pasteurella pneumotropica, aeromo ... | 2014 | 24955522 |
| [microbial composition of various surfaces of skin during develop- ment of atopic dermatitis based on data from maldi-tof mass-spectrometry identification method]. | study transformation of skin microflora during development of atopic dermatitis. | 2016 | 27228668 |
| nicotine-degrading microorganisms and their potential applications. | nicotine-degrading microorganisms (ndms) are a special microbial group which can use nicotine as the sole carbon and nitrogen source for growth. since the 1950s, the bioconversion of nicotine by microbes has received increasing attention, and several ndms have been identified, such as arthrobacter nicotinovorans, microsporum gypseum, pellicularia filamentosa jts-208, and pseudomonas sp. 41. in recent years, increasing numbers of ndms have been isolated and identified from tobacco plantation soil ... | 2015 | 25805341 |
| efficacy of root-associated fungi and pgpr on the growth of pisum sativum (cv. arkil) and reproduction of the root-knot nematode meloidogyne incognita. | the effects of root-associated fungi (aspergillus awamori and glomus mosseae) and plant growth promoting rhizobacteria (pgpr) (pseudomonas putida, pseudomonas alcaligenes and paenibacillus polymyxa) were studied alone and in combination in glasshouse experiments on the growth of pea, enzyme activity (peroxidase and catalase) and reproduction of root-knot nematode meloidogyne incognita. application of a. awamori, g. mosseae and pgpr caused a significant increase in pea growth and enzyme activitie ... | 2013 | 22915081 |
| bioaugmented composting of jatropha de-oiled cake and vegetable waste under aerobic and partial anaerobic conditions. | this study was conducted to assess the effect of microbial inoculation in jatropha cake composting with different vegetable waste. the microbial inoculums composed of fungal strains (aspergillus awamori, aspergillus nidulans, trichoderma viride, phanerochaete chrysosporium) and bacterial inoculums (pseudomonas striata as phosphorus solublizer and azotobacter chroococcum as nitrogen fixer) were added to the compost mixture after the thermophilic phase was over for bioaugmenting of jatropha cake u ... | 2013 | 22736484 |
| molecular and spectroscopic characterization of aspergillus flavipes and pseudomonas putida l-methionine γ-lyase in vitro. | pseudomonas putida l-methionine γ-lyase (ppmgl) has been recognized as an efficient anticancer agent, however, its antigenicity and stability remain as critical challenges for its clinical use. from our studies, aspergillus flavipes l-methionine γ-lyase (afmgl) displayed more affordable biochemical properties than ppmgl. thus, the objective of this work was to comparatively assess the functional properties of afmgl and ppmgl via stability of their internal aldimine linkage, tautomerism of pyrido ... | 2016 | 27796875 |
| pharmacokinetics, immunogenicity and anticancer efficiency of aspergillus flavipes l-methioninase. | methionine starvation can powerfully modulate dna methylation, cell cycle transition, polyamines and antioxidant synthesis of tumor cells, in contrary to normal ones. aspergillus flavipesl-methioninase was previously characterized by our studies, displaying affordable biochemical properties comparing to pseudomonas putida enzyme (oncase). thus, the objective of current study was to evaluate the catalytic properties of af-metase in new zealand rabbits, exploring its antitumor efficacy. in vivo, a ... | 2012 | 22883554 |
| rice-field drowning-associated pneumonia in which pseudomonas spp., aspergillus fumigatus, and cunninghamella sp. are isolated. | we herein report the case of an 84-year-old who developed pneumonia after drowning in a rice field. besides aspergillus fumigatus, many pathogens previously not reported in drowning-associated pneumonia (such as pseudomonas fluorescens, pseudomonas putida, nocardia niigatensis, and cunninghamella sp.) were isolated from his sputum. he received sulbactam/ampicillin, trimethoprim/sulfamethoxazole, voriconazole, levofloxacin and liposomal amphotericin b, but died due to respiratory failure. because ... | 2016 | 27041173 |
| enantioselective resolution of (r,s)-carvedilol to (s)-(-)-carvedilol by biocatalysts. | among the microorganisms employed in the study, aspergillus niger (gufcc5443), escherichia coli (atcc9637), streptomyces halstedii (ckm-2), pseudomonas putida (ncib9494), cunninghamella elegans (ncim689) and sphingomonas paucimobilis (nctc11030) were capable for the enantioselective conversion of racemic carvedilol. immobilization technique enhanced the enantioselectivity of microorganisms and thus increased the enantiomeric purity of the drug. excellent enantiomeric ratios (e) were found in rea ... | 2017 | 28064425 |
| rational design, synthesis, anti-hiv-1 rt and antimicrobial activity of novel 3-(6-methoxy-3,4-dihydroquinolin-1(2h)-yl)-1-(piperazin-1-yl)propan-1-one derivatives. | in the present study, fifteen novel 3-(6-methoxy-3,4-dihydroquinolin-1(2h)-yl)-1-(piperazin-1-yl)propan-1-one (6a-o) derivatives were designed as inhibitor of hiv-1 rt using ligand based drug design approach and in-silico evaluated for drug-likeness properties. designed compounds were synthesized, characterized and in-vitro evaluated for rt inhibitory activity against wild hiv-1 rt strain. among the tested compounds, four compounds (6a, 6b, 6j and 6o) exhibited significant inhibition of hiv-1 rt ... | 2016 | 27288643 |
| mechanism-specific and whole-organism ecotoxicity of mono-rhamnolipids. | biosurfactants like rhamnolipids are promising alternatives to chemical surfactants in a range of applications. a wider use requires an analysis of their environmental fate and their ecotoxicological potential. in the present study mono-rhamnolipids produced by a recombinant pseudomonas putida strain were analyzed using the green toxicology concept for acute and mechanism-specific toxicity in an ecotoxicological test battery. acute toxicity tests with the invertebrate daphnia magna and with zebr ... | 2016 | 26802344 |
| vanadium removal from ld converter slag using bacteria and fungi. | removal of vanadium from linz-donawits (ld) converter slag was investigated by means of three different species of microbial systems: acidithiobacillus thiooxidans (autotrophic bacteria), pseudomonas putida (heterotrophic bacteria) and aspergillus niger (fungi). the bioleaching process was carried out in both one-step and two-step process and the leaching efficiencies in both cases were compared. formation of inorganic and organic acids during the leaching process caused mobilization of vanadium ... | 2015 | 25697901 |
| interesterification of butter fat by partially purified extracellular lipases from pseudomonas putida, aspergillus niger and rhizopus oryzae. | three extracellular lipases were produced by batch fermentation of pseudomonas putida atcc 795, aspergillus niger cbs 131.52 and rhizopus oryzae atcc 34612 during the late phase of growth, at 72, 96 and 96 h, respectively. the lipases were partially purified by (nh4)2so4 fractionation. the lipase of p. putida was optimal at ph 8.0 whereas those from a. niger and r. oryzae were optimal at ph 7.5. the a. niger lipase had the lowest v max value (0.51×10(-3) u/min) and r. oryzae the highest (1.86×10 ... | 1995 | 24415019 |
| extraordinary solute-stress tolerance contributes to the environmental tenacity of mycobacteria. | mycobacteria are associated with a number of well-characterized diseases, yet we know little about their stress biology in natural ecosystems. this study focuses on the isolation and characterization of strains from yellowstone national park (ynp) and glacier national park (gnp; usa), the majority of those identified were mycobacterium parascrofulaceum, mycobacterium avium (ynp) or mycobacterium gordonae (gnp). generally, their windows for growth spanned a temperature range of > 60 °c; selected ... | 2015 | 26059202 |
| a bioinformatics approach to the determination of genes involved in endophytic behavior in burkholderia spp. | the vast majority of plants harbor endophytic bacteria that colonize a portion of the plant's interior tissues without harming the plant. like plant pathogens, endophytes gain entry into their plants hosts through various mechanisms. bacterial endophytes display a broad range of symbiotic interactions with their host plants. the molecular bases of these plant-endophyte interactions are currently not fully understood. in the present study, a set of genes possibly responsible for endophytic behavi ... | 2014 | 24513137 |
| preparation and biological activity of new collagen composites, part i: collagen/zinc titanate nanocomposites. | the aim of this investigation was to develop new antimicrobial collagen/zinc titanate (zntio3) biomaterials using a sol-gel cryogenic draying technology in keeping the native collagen activity. broad-spectrum antimicrobial activity was demonstrated against firmicutes (staphylococcus epidermidis, bacillus cereus, and candida lusitaniae) and gracilicutes (escherichia coli, salmonella enterica, and pseudomonas putida) microorganisms. the antimicrobial activity as well as the cytotoxicity were speci ... | 2016 | 27138724 |
| effect of fermented broth from lactic acid bacteria on pathogenic bacteria proliferation. | in this study, the effect that 5 fermented broths of lactic acid bacteria (lab) strains have on the viability or proliferation and adhesion of 7 potentially pathogenic microorganisms was tested. the fermented broth from lactococcus lactis c660 had a growth inhibitory effect on escherichia coli k92 that reached of 31%, 19% to pseudomonas fluorescens, and 76% to staphylococcus epidermidis. the growth of staph. epidermidis was negatively affected to 90% by lc. lactis 11454 broth, whereas the growth ... | 2016 | 26851857 |
| isolation and characterization of bacterial endophytes of curcuma longa l. | fourteen endophytic bacterial isolates were isolated from the rhizome of curcuma longa l. were characterized on the basis of morphology, biochemical characteristics and 16s rrna gene sequence analysis. the isolates were identified to six strains namely bacillus cereus (ecl1), bacillus thuringiensis (ecl2), bacillus sp. (ecl3), bacillus pumilis (ecl4), pseudomonas putida (ecl5), and clavibacter michiganensis (ecl6). all the strains produced iaa and solubilized phosphate and only two strains produ ... | 2016 | 28330130 |
| pyrosequencing analysis of microbial community and food-borne bacteria on restaurant cutting boards collected in seri kembangan, malaysia, and their correlation with grades of food premises. | this study adopts the pyrosequencing technique to identify bacteria present on 26 kitchen cutting boards collected from different grades of food premises around seri kembangan, a city in malaysia. pyrosequencing generated 452,401 of total reads of otus with an average of 1.4×10(7) bacterial cells/cm(2). proteobacteria, firmicutes and bacteroides were identified as the most abundant phyla in the samples. taxonomic richness was generally high with >1000 operational taxonomic units (otus) observed ... | 2015 | 25679309 |
| dechlorination of chloroorganics, decolorization, and simultaneous bioremediation of cr6+ from real tannery effluent employing indigenous bacillus cereus isolate. | a native bacillus cereus isolate has been employed, for the first time, for simultaneous decolorization, dechlorination of chloroorganics, and cr(6+) remediation from the real tannery effluent. most of the physicochemical variables in 3:1 diluted effluent were well above the standard prescribed limits, which decreased substantially upon microbial treatment. the extent of bioremediation was better in diluted (3:1) as compared to undiluted effluent supplemented with nutrients and augmented with b. ... | 2014 | 24390198 |
| community composition and cellulase activity of cellulolytic bacteria from forest soils planted with broad-leaved deciduous and evergreen trees. | cellulolytic bacteria in forest soil provide carbon sources to improve the soil fertility and sustain the nutrient balance of the forest ecological system through the decomposition of cellulosic remains. these bacteria can also be utilized for the biological conversion of biomass into renewable biofuels. in this study, the community compositions and activities of cellulolytic bacteria in the soils of forests planted with broad-leaved deciduous (chang qing garden, cqg) and broad-leaved evergreen ... | 2014 | 23893311 |
| anti-biofilm forming and anti-quorum sensing activity of selected essential oils and their main components on food-related micro-organisms. | the aim of this study was to investigate the effect of clary sage, juniper, lemon and marjoram essential oils (eos) and their major components on the formation of bacterial and yeast biofilms and on the inhibition of ahl-mediated quorum sensing (qs). | 2013 | 23789847 |
| novel polymer anchored cr(iii) schiff base complexes: synthesis, characterization and antimicrobial properties. | new polymer-bound schiff bases and cr(iii) complexes have been synthesized by the reaction of 4-benzyloxybenzaldehyde, polymer-bound with 2-aminophenol, 2-amino-4-chlorophenol and 2-amino-4-methylphenol. the structure of polymeric-schiff bases and their cr(iii) complexes have been characterized by elemental analyses, magnetic measurements, ir, uv-vis, tg-dta and (1)h-nmr. all these compounds have also been investigated for antibacterial activity by the well-diffusion method against staphylococcu ... | 2012 | 22622060 |
| a preliminary study on the antibacterial mechanism of tegillarca granosa hemoglobin by derived peptides and peroxidase activity. | the blood clam, tegillarca granosa, is one of the few bivalve molluscs containing hemoglobin (hb). in the present study, we purified two types of t. granosa hemoglobin, tg-hbi and tg-hbii, using size exclusion chromatography and measured their antibacterial and peroxidase activities. we also tested antibacterial activities of peptides prepared by trypsin digestion of purified tg-hb and reversed-phase high-performance liquid chromatography purification. purified tg-hbi and tg-hbii showed antibact ... | 2016 | 26876330 |
| the ability of consortium wastewater protozoan and bacterial species to remove cod in the presence of nanomaterials under varying ph conditions. | the aim of this study was to ascertain the survival limit and capability of commonly found wastewater protozoan (aspidisca sp, trachelophyllum sp and peranema sp) and bacterial (bacillus licheniformis, brevibacillus laterosporus and pseudomonas putida) species to remove cod while exposed to commercial nanomaterials under varying ph conditions. the experimental study was carried out in modified mixed liquor media adjusted to various ph levels (ph 2, 7 and 10) and a comparative study was performed ... | 2017 | 28368692 |
| effect of vanadium toxicity at its different oxidation states on selected bacterial and protozoan isolates in wastewater systems. | this study assesses and compares vanadium toxicity in its different oxidation states towards bacterial isolates (pseudomonas putida and bacillus licheniformis) and protozoan isolates (peranema sp. and trachelophyllum sp.). the isolates were exposed to various concentrations of v in mixed liquors and their tolerance to v was assessed at 30 degrees c at a ph of 4. the results revealed that the increase in v oxidation state increased its toxicity to bacterial isolates, whereas its toxicity decrease ... | 2014 | 24956802 |
| soil bacterial consortia and previous exposure enhance the biodegradation of sulfonamides from pig manure. | persistence or degradation of synthetic antibiotics in soil is crucial in assessing their environmental risks. microbial catabolic activity in a sandy loamy soil with pig manure using 12c- and 14c-labelled sulfamethazine (smz) respirometry showed that smz was not readily degradable. but after 100 days, degradation in sulfadiazine-exposed manure was 9.2%, far greater than soil and organic manure (0.5% and 0.11%, respectively, p < 0.05). abiotic degradation was not detected suggesting microbial ca ... | 2012 | 22286498 |
| contribution of arbuscular mycorrhizal fungi and/or bacteria to enhancing plant drought tolerance under natural soil conditions: effectiveness of autochthonous or allochthonous strains. | autochthonous microorganisms [a consortium of arbuscular-mycorrhizal (am) fungi and bacillus thuringiensis (bt)] were assayed and compared to rhizophagus intraradices (ri), bacillus megaterium (bm) or pseudomonas putida (psp) and non-inoculation on trifolium repens in a natural arid soil under drought conditions. the autochthonous bacteria bt and the allochthonous bacteria psp increased nutrients and the relative water content and decreased stomatal conductance, electrolyte leakage, proline and ... | 2015 | 25462971 |
| controlled oxidation of aliphatic ch bonds in metallo-monooxygenases: mechanistic insights derived from studies on deuterated and fluorinated hydrocarbons. | the control over the regio- and/or stereo-selective aliphatic ch oxidation by metalloenzymes is of great interest to scientists. typically, these enzymes invoke host-guest chemistry to sequester the substrates within the protein pockets, exploiting sizes, shapes and specific interactions such as hydrogen-bonding, electrostatic forces and/or van der waals interactions to control the substrate specificity, regio-specificity and stereo-selectivity. over the years, we have developed a series of deut ... | 2014 | 24629413 |
| impact of carbon source and variable nitrogen conditions on bacterial biosynthesis of polyhydroxyalkanoates: evidence of an atypical metabolism in bacillus megaterium dsm 509. | twenty bacterial strains were examined on their ability to produce polyhydroxyalkanoates (pha) from different carbon sources under rich and depleted nitrogen conditions. preliminary experiments with glucose as sole carbon source allowed to select pha producing bacteria using ftir spectroscopy. they were further tested with eight additional carbon substrates including organic, fatty acids or sugars. pha content and monomer composition of four chosen strains (pseudomonas putida mt-2, bacillus mega ... | 2013 | 23548274 |
| effect of plant growth-promoting rhizobacteria (pgprs) on plant growth, yield, and quality of tomato (lycopersicon esculentum mill.) under simulated seawater irrigation. | to determine the effects of three pgprs on plant growth, yield, and quality of tomato under simulated seawater irrigation, a two consecutive seasons' field experiment was conducted in yancheng teachers university plot from april to june and august to october, 2011. the results showed that erwinia persicinus ra2 containing acc deaminase exhibited the best ability compared with bacillus pumilus wp8 and pseudomonas putida rbp1 which had no acc deaminase activity to enhance marketable yields of fres ... | 2012 | 22990485 |
| novel tools for the functional expression of metagenomic dna. | functional expression of genes from metagenomic libraries is limited by various factors including inefficient transcription and/or translation of target genes as well as improper folding and assembly of the corresponding proteins caused by the lack of appropriate chaperones and cofactors. it is now well accepted that the use of different expression hosts of distinct phylogeny and physiology can dramatically increase the rate of success. in the following chapter, we therefore describe tools and p ... | 2017 | 27900689 |
| influence of bacterial extracellular polymeric substances on the sorption of zn on γ-alumina: a combination of ftir and exafs studies. | extracellular polymeric substances (eps) isolated from bacteria, are abound of functional groups which can react with metals and consequently influence the immobilization of metals. in this study, we combined with zn k-edge extended x-ray absorption fine structure (exafs), fourier transform infrared (ftir) spectroscopy, and high-resolution transmission electron microscopy (hrtem) techniques to study the effects of eps isolated from bacillus subtilis and pseudomonas putida on zn sorption on γ-alu ... | 2017 | 27876416 |
| inhibited transport of graphene oxide nanoparticles in granular quartz sand coated with bacillus subtilis and pseudomonas putida biofilms. | increasing production and use of graphene oxide nanoparticles (gonps) boost their wide dissemination in the subsurface environments where biofilms occur ubiquitously, representative of the physical and chemical heterogeneities. this study aimed at investigating the influence of gram-positive bacillus subtilis (bs) and gram-negative pseudomonas putida (pp) biofilms on the transport of gonps under different ionic strengths (0.1, 0.5, and 1.0 mm cacl2) at neutral ph 7.2 in water-saturated porous me ... | 2017 | 27855326 |
| aquatic toxicity and biodegradability of a surfactant produced by bacillus subtilis ica56. | in this work, the environmental compatibility of a biosurfactant produced by a bacillus subtilis strain isolated from the soil of a brazilian mangrove was investigated. the biosurfactant, identified as surfactin, is able to reduce surface tension (st) to 31.5 ± 0.1 mn m(-1) and exhibits a lowcritical micelle concentration (cmc) value (0.015 ± 0.003 g l(-1)). the highest crude biosurfactant concentration (224.3 ± 1.9 mg l(-1)) was reached at 72 h of fermentation. acute toxicity tests, carried out ... | 2017 | 27791474 |
| bacteria induce pigment formation in the basidiomycete serpula lacrymans. | basidiomycete fungi are characterized ecologically for their vital functional role in ecosystem carbon recycling and chemically for their capacity to produce a diverse array of small molecules. chromophoric natural products derived from the quinone precursor atromentin, such as variegatic acid and involutin, have been shown to function in redox cycling. yet, in the context of an inter-kingdom natural system these pigments are still elusive. here, we co-cultured the model saprotrophic basidiomyce ... | 2016 | 27699944 |
| purification and properties of glycine oxidase from pseudomonas putida kt2440. | glycine oxidase, encoded by the thio gene, participates in the biosynthesis of thiamin by providing glyoxyl imine to form the thiazole moiety of thiamin. we have purified and characterized thio from pseudomonas putida kt2440. it has a monomeric structure that is distinct from the homotetrameric thios from bacillus subtilis and geobacillus kaustophilus. the p. putida thio is unique in that glycine is its preferred substrate, which differs markedly from the b. subtilis and g. kaustophilus enzymes ... | 2015 | 26875494 |
| mcpq is a specific citrate chemoreceptor that responds preferentially to citrate/metal ion complexes. | chemoreceptors are at the beginning of chemosensory pathways that mediate chemotaxis. pseudomonas putida kt2440 is predicted to have 27 chemoreceptors, most of which uncharacterized. we have previously identified mcps as chemoreceptor for krebs cycle intermediates. citrate is primarily present in the environment as metal complex, which, however, is not recognized by mcps. we show here that the mcps paralogue mcpq recognizes specifically citrate and citrate/metal(2+) complexes. the mcpq ligand bi ... | 2016 | 26463109 |