| retail survey of brazilian milk and minas frescal cheese and a contaminated dairy plant to establish prevalence, relatedness, and sources of listeria monocytogenes isolates. | a study was designed to recover listeria monocytogenes from pasteurized milk and minas frescal cheese (mfc) sampled at retail establishments (res) and to identify the contamination source(s) of these products in the corresponding dairy processing plant. fifty milk samples (9 brands) and 55 mfc samples (10 brands) were tested from res located in juiz de fora, minas gerais, brazil. all milk samples and 45 samples from 9 of 10 mfc brands tested negative for l. monocytogenes; however, "brand f" of m ... | 2008 | 18502929 |
| listeria monocytogenes egd-e biofilms: no mushrooms but a network of knitted chains. | listeria monocytogenes is a food pathogen that can attach on most of the surfaces encountered in the food industry. biofilms are three-dimensional microbial structures that facilitate the persistence of pathogens on surfaces, their resistance toward antimicrobials, and the final contamination of processed goods. so far, little is known about the structural dynamics of l. monocytogenes biofilm formation and its regulation. the aims of this study were, by combining genetics and time-lapse laser-sc ... | 2008 | 18502930 |
| connatal listeriosis--a case report and the possibilities of microbiological diagnosis. | the third most frequent agent of perinatal bacterial meningitis is listeria monocytogenes, in hungary, its occurrence is, however, uncommon. this raises the possibility of diagnostical mistakes. a connatal listeriosis case validated microbiologically referred to in this report calls attention to listeria, as a rare but relevant pathogen of neonatal infections. if clinical background suggests infection, the pathogenic role of l. monocytogenes should be taken into consideration. the etiological si ... | 2008 | 18507152 |
| capping protein increases the rate of actin-based motility by promoting filament nucleation by the arp2/3 complex. | capping protein (cp) is an integral component of arp2/3-nucleated actin networks that drive amoeboid motility. increasing the concentration of capping protein, which caps barbed ends of actin filaments and prevents elongation, increases the rate of actin-based motility in vivo and in vitro. we studied the synergy between cp and arp2/3 using an in vitro actin-based motility system reconstituted from purified proteins. we find that capping protein increases the rate of motility by promoting more f ... | 2008 | 18510928 |
| a laboratory study of survival of selected microorganisms after heat treatment of biowaste used in biogas plants. | the aim of the study was to assess the effect of pasteurisation, as set by the european regulation ec 1774/2002, on selected pathogens and indicator organisms. unpasteurised substrate (biowaste), including animal by-products from a full-scale biogas plant was heat treated under laboratory conditions at 70 degrees c and 55 degrees c for 30 min and 60 min. heat treatment at 55 degrees c for 60 min was not sufficient to achieve a hygienically acceptable product. heat treatment at 70 degrees c for 3 ... | 2008 | 18513960 |
| listeria monocytogenes biofilm-associated protein (bapl) may contribute to surface attachment of l. monocytogenes but is absent from many field isolates. | listeria monocytogenes is a food-borne pathogen capable of adhering to a range of surfaces utilized within the food industry, including stainless steel. the factors required for the attachment of this ubiquitous organism to abiotic surfaces are still relatively unknown. in silico analysis of the l. monocytogenes egd genome identified a putative cell wall-anchored protein (lmo0435 [bapl]), which had similarity to proteins involved in biofilm formation by staphylococci. an insertion mutation was c ... | 2008 | 18515491 |
| validation of a commercial process for inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes on the surface of whole muscle beef jerky. | we validated the lethality of three time and temperature regimens for commercial processing of whole muscle beef jerky. a total of ca. 8.9 log cfu per strip of multiple-strain cocktails of escherichia coli o157:h7, salmonella typhimurium, or listeria monocytogenes were separately applied onto the surface of beef strips that were treated as follows: (i) inoculated but not marinated or (ii) inoculated and then marinated. a total of three beef strips for each treatment in each of three trials were ... | 2008 | 18522024 |
| a processing plant persistent strain of listeria monocytogenes crosses the fetoplacental barrier in a pregnant guinea pig model. | the foodborne pathogen listeria monocytogenes can cause infection in immunocompromised humans and in the fetuses of pregnant women. we have demonstrated that one group of genetically similar l. monocytogenes strains (random amplified polymorphic dna [rapd] type 9) dominate and persist in several independent fish processing plants. the purpose of the present study was to determine the virulence potential of one rapd type 9 strain (la111), one human clinical strain (scott a), and one monkey clinic ... | 2008 | 18522041 |
| dependency of caspase-1 activation induced in macrophages by listeria monocytogenes on cytolysin, listeriolysin o, after evasion from phagosome into the cytoplasm. | listeriolysin o (llo), an hly-encoded cytolysin from listeria monocytogenes, plays an essential role in the entry of this pathogen into the macrophage cytoplasm and is also a key factor in inducing the production of ifn-gamma during the innate immune stage of infection. in this study, we examined the involvement of llo in macrophage production of the ifn-gamma-inducing cytokines il-12 and il-18. significant levels of il-12 and il-18 were produced by macrophages upon infection with wild-type l. m ... | 2008 | 18523249 |
| molecular mimicry between neurons and an intracerebral pathogen induces a cd8 t cell-mediated autoimmune disease. | to identify basic mechanisms of how infections may induce a neuron-specific autoimmune response, we generated mice expressing ova as neuronal autoantigen under control of the neuron-specific enolase promoter (nse-ova mice). intracerebral, but not systemic, infection with attenuated listeria monocytogenes-secreting ova induced an atactic-paretic neurological syndrome in nse-ova mice after bacterial clearance from the brain, whereas wild-type mice remained healthy. immunization with attenuated lis ... | 2008 | 18523310 |
| listeria monocytogenes meningitis associated with rhabdomyolysis and acute renal failure. | numerous systemic infections are capable of inducing myositis and rhabdomyolysis. clinical course of the disease is in the great majority of patients benign and without development of renal dysfunction. however, serious consequences are possible if acute renal failure (arf) occurs, especially in critically ill patients. | 2009 | 18528786 |
| listeria monocytogenes and staphylococcus aureus coinfection of a prosthetic joint. | | 2008 | 18534891 |
| antimicrobial activity of bacterial isolates from different floral sources of honey. | more than two thousand bacterial strains isolated from six us domestic honeys and two manuka honeys from new zealand were screened for production of antimicrobial compounds. a high incidence of antimicrobial inhibition determined by deferred inhibition assays was observed with the bacterial isolates from all eight honey samples. in total, 2217 isolates out of 2398 strains (92.5% of total isolates) exhibited antimicrobial activity against at least one of the tested microorganisms. antifungal acti ... | 2008 | 18538876 |
| sel, a selective enrichment broth for simultaneous growth of salmonella enterica, escherichia coli o157:h7, and listeria monocytogenes. | multipathogen detection on a single-assay platform not only reduces the cost for testing but also provides data on the presence of pathogens in a single experiment. to achieve this detection, a multipathogen selective enrichment medium is essential to allow the concurrent growth of pathogens. sel broth was formulated to allow the simultaneous growth of salmonella enterica, escherichia coli o157:h7, and listeria monocytogenes. the results were compared to those obtained with the respective indivi ... | 2008 | 18539786 |
| isolation and partial characterization of a bacteriocin produced by pediococcus pentosaceus k23-2 isolated from kimchi. | screening and partial characterization of a bacteriocin produced by pediococcus pentosaceus k23-2 isolated from kimchi, a traditional korean fermented vegetable. | 2008 | 18540969 |
| growth and persistence of listeria monocytogenes isolates on the plant model arabidopsis thaliana. | while the majority of human listeriosis cases appear to be linked to consumption of processed ready-to-eat foods (e.g., deli meats), a few listeriosis outbreaks have been linked to consumption of contaminated vegetables. in this study, we assessed four isolates representing the major listeria monocytogenes lineages for their abilities to attach to and grow on arabidopsis thaliana, a well-characterized plant model. when plants were dipped for 5min into 3ml of water containing 8.8logcfu of l. mono ... | 2008 | 18541169 |
| chemical composition and inhibitory parameters of essential oil and extracts of nandina domestica thunb. to control food-borne pathogenic and spoilage bacteria. | the aim of this study was to examine the chemical composition of the essential oil isolated from the floral parts of nandina domestica thunb. by hydrodistillation, and to test the efficacy of essential oil and various organic extracts against a panel of food-borne pathogenic and spoilage bacteria such as bacillus subtilis atcc6633, listeria monocytogenes atcc19166, staphylococcus aureus kctc1916, s. aureus atcc6538, pseudomonas aeruginosa kctc2004, salmonella typhimurium kctc2515, salmonella ent ... | 2008 | 18541324 |
| constitutive activation of the prfa regulon enhances the potency of vaccines based on live-attenuated and killed but metabolically active listeria monocytogenes strains. | recombinant vaccines derived from the facultative intracellular bacterium listeria monocytogenes are presently undergoing early-stage clinical evaluation in oncology treatment settings. this effort has been stimulated in part due to preclinical results that illustrate potent activation of innate and adaptive immune effectors by l. monocytogenes vaccines, combined with efficacy in rigorous animal models of malignant and infectious disease. here, we evaluated the immunologic potency of a panel of ... | 2008 | 18541651 |
| a new twist on an old pathway--accessory sec [corrected] systems. | the export of proteins from their site of synthesis in the cytoplasm across the inner membrane is an important aspect of bacterial physiology. because the location of extracytoplasmic proteins is ideal for host-pathogen interactions, protein export is also important to bacterial virulence. in bacteria, there are conserved protein export systems that are responsible for the majority of protein export: the general secretion (sec) pathway and the twin-arginine translocation pathway. in some bacteri ... | 2008 | 18544071 |
| shelf life evaluation for ready-to-eat sliced uncured turkey breast and cured ham under probable storage conditions based on listeria monocytogenes and psychrotroph growth. | the growth variability of three listeria monocytogenes ribotypes in ready-to-eat (rte) sliced uncured turkey breast and cured ham was studied under storage conditions that rte foods are likely to encounter. three product treatments studied were: (1) a control; (2) a formulation subjected to high pressure processing to reduce initial microbial load (hpp); (3) a formulation containing 2.0% potassium lactate and 0.2% sodium diacetate (pl/sd). after separate inoculation with individual l. monocytoge ... | 2008 | 18544466 |
| contrasting effects of human thp-1 cell differentiation on levofloxacin and moxifloxacin intracellular accumulation and activity against staphylococcus aureus and listeria monocytogenes. | listeria monocytogenes and staphylococcus aureus invade and multiply in thp-1 monocytes. fluoroquinolones accumulate in these cells, but are less active against intracellular than extracellular forms of l. monocytogenes and s. aureus. we examined whether differentiation of thp-1 monocytes into adherent, macrophage-like cells increases fluoroquinolone uptake and activity. | 2008 | 18544595 |
| urinary retention secondary to listeria meningitis. | we report a rare case of urinary retention secondary to listeria meningitis. a 90-year-old woman presented with high fever, nausea, diarrhea, and incontinence of urination and feces. lumbar puncture was performed. the total cell number of the cerebrospinal fluid (csf) was elevated indicating that the glucose level was decreased. a csf culture and a blood culture revealed listeria monocytogenes (l. monocytogenes). we treated this bacterial meningitis with antibiotic medicine. one month after onse ... | 2008 | 18552472 |
| development of multiple strain competitive index assays for listeria monocytogenes using pimc; a new site-specific integrative vector. | the foodborne, gram-positive pathogen, listeria monocytogenes, is capable of causing lethal infections in compromised individuals. in the post genomic era of l. monocytogenes research, techniques are required to identify and validate genes involved in the pathogenicity and environmental biology of the organism. the aim here was to develop a widely applicable method to tag l. monocytogenes strains, with a particular emphasis on the development of multiple strain competitive index assays. | 2008 | 18554399 |
| interactions in dual species biofilms between listeria monocytogenes egd-e and several strains of staphylococcus aureus. | six environmental isolates of staphylococcus aureus and one collection strain were investigated for their ability to form monospecies biofilms and dual species biofilms with listeria monocytogenes egd-e on stainless steel coupons. all isolates were able to grow as biofilms but their ability to form monospecies biofilms differed. the population of l. monocytogenes egd-e in dual species biofilms was not affected by the presence of s. aureus isolates except for strain cip 53.156. the effect of l. m ... | 2008 | 18554739 |
| photoactivated chlorophyllin-based gelatin films and coatings to prevent microbial contamination of food products. | the aim of this work was to develop antimicrobial photosensitizer-containing edible films and coatings based on gelatin as the polymer matrix, incorporating sodium magnesium chlorophyllin (e-140) and sodium copper chlorophyllin (e-141). chlorophyllins were incorporated into the gelatin film-forming solution and the inhibiting effect of the cast films was tested against staphylococcus aureus and listeria monocytogenes. the results demonstrated that water soluble sodium magnesium chlorophyllin and ... | 2008 | 18555550 |
| growth of l. monocytogenes strain f2365 on ready-to-eat turkey meat does not enhance gastrointestinal listeriosis in intragastrically inoculated a/j mice. | there have been significant outbreaks of listeriosis associated with consumption of contaminated ready-to-eat (rte) turkey meat products. in this study, we investigated whether growth on rte deli turkey meat sends environmental signals to listerial cells that makes them more virulent in the gastrointestinal tract of mice. l. listeria monocytogenes strain f2365 grew from a starting inoculum of 10(3) cfu/ml to final numbers of 10(8)-10(9) cfu/ml (within 12 days at 10 degrees c) when inoculated ont ... | 2008 | 18559288 |
| a listeria monocytogenes mutant defective in bacteriophage attachment is attenuated in orally inoculated mice and impaired in enterocyte intracellular growth. | a listeria monocytogenes bacteriophage was used to identify a phage-resistant tn917 insertion mutant of the mouse-virulent listerial strain f6214-1. the mutant was attenuated when it was inoculated orally into female a/j mice and failed to replicate efficiently in cultured mouse enterocytes. phage binding studies indicated that the mutant had a cell surface alteration that precluded phage attachment. all phenotypes associated with the mutation could be complemented in trans by a single open read ... | 2008 | 18559424 |
| curvature and torsion in growing actin networks. | intracellular pathogens such as listeria monocytogenes and rickettsia rickettsii move within a host cell by polymerizing a comet-tail of actin fibers that ultimately pushes the cell forward. this dense network of cross-linked actin polymers typically exhibits a striking curvature that causes bacteria to move in gently looping paths. theoretically, tail curvature has been linked to details of motility by considering force and torque balances from a finite number of polymerizing filaments. here we ... | 2008 | 18560043 |
| structural analysis of the l-alanoyl-d-glutamate endopeptidase domain of listeria bacteriophage endolysin ply500 reveals a new member of the las peptidase family. | similar to many other bacterial cell-wall-hydrolyzing enzymes, the listeria bacteriophage a500 endopeptidase ply500 has a modular architecture consisting of an enzymatically active domain (ead) linked to a cell-wall-binding domain (cbd) in a single polypeptide chain. the crystal structure of the ead of ply500 has been solved at 1.8 a resolution. the shape of the enzyme resembles a sofa chair: one alpha-helix and three antiparallel beta-strands form the seat, which is supported by two more alpha- ... | 2008 | 18560152 |
| listeria monocytogenes peritonitis in a patient on peritoneal dialysis: a case report and review of the literature. | listeria monocytogenes (lm) is one of the rare microorganisms causing peritonitis in peritoneal dialysis (pd) patients. we report a sporadic case of peritonitis caused by lm in a young female pd patient with lupus receiving corticosteroid therapy, who presented with abdominal pain, cloudy pd effluent, nausea, and conjunctivitis. the effluent showed a high pd effluent white cell count and monocytosis, and gram staining showed gram-positive bacilli in single or short chains and pd effluent culture ... | 2008 | 18563614 |
| how to manage infections in the era of biologics? | biologic agents are immunosuppressants that target cytokines or specific immune cell subpopulations. many therapies interfere with the normal inflammatory cascade and with the immune system, causing an increase in the incidence of infections. in particular, treatment with tumor necrosis factor (tnf)-alpha antagonists in psoriasis patients is associated with an increased risk of infection caused by intracellular microorganisms. tnf-alpha plays an important role in host resistance against infectio ... | 2008 | 18564248 |
| antimicrobial resistance of listeria monocytogenes isolated from a poultry further processing plant. | the aim of this study was to compare antimicrobial resistance profiles of distinct types of listeria monocytogenes isolated from a commercial poultry cooking plant. one hundred fifty-seven l. monocytogenes isolates representing 14 different acta types were tested for susceptibility to 19 antimicrobial drugs using the broth microdilution method. all isolates were susceptible to antimicrobials except for ceftriaxone (153 isolates [97%] intermediate or resistant), oxacillin with 2% nacl (142 isolat ... | 2008 | 18564907 |
| sigmab- and prfa-dependent transcription of genes previously classified as putative constituents of the listeria monocytogenes prfa regulon. | mounting evidence suggests that sigma(b) and prfa coregulate transcription of multiple genes in listeria monocytogenes, therefore, the relative contributions of sigma(b) and prfa to transcript levels of genes identified previously as differentially regulated by prfa were measured. group i genes are recognized virulence genes that are positively regulated by prfa; group ii genes were reported previously as negatively regulated by prfa; and multiple group iii genes were proposed to be coregulated ... | 2008 | 18564909 |
| analysis of survival rates and cellular fatty acid profiles of listeria monocytogenes treated with supercritical carbon dioxide under the influence of cosolvents. | in the present study, we identified several process variables that significantly affect the efficiency of supercritical carbon dioxide inactivation of the food-borne pathogen listeria monocytogenes. treatment with sc-co(2) completely disabled the colony-forming activity of the cells (8-log reduction) within specific treatment time (10-50 min), pressure (80-150 bar), and temperature ranges (35-45 degrees c). microorganism inactivation rates increased proportionally with pressure and temperature, ... | 2008 | 18565606 |
| immunity gene pedb enhances production of pediocin pa-1 in naturally resistant lactococcus lactis strains. | heterologous production of the antilisterial bacteriocin pediocin pa-1 in lactococci is an attractive objective to increase the safety of dairy products. in a previous paper, we developed a system for the heterologous production of the bacteriocin pediocin pa-1 in pediocin-resistant lactococcal hosts through a leader exchange strategy. the system was based on 3 genes, 1 encoding the fusion between the lactococcin a leader and propediocin pa-1, and the other 2 encoding the lactococcin a secretion ... | 2008 | 18565917 |
| innate responses to systemic infection by intracellular bacteria trigger recruitment of ly-6chigh monocytes to the brain. | blood borne listeria monocytogenes enter the cns via migration of parasitized ly-6chigh monocytes, but the signals that trigger this migration are not known. to understand more completely events leading to monocyte recruitment, experiments presented here combined microarray analysis of gene expression in the brains of experimentally infected mice with measurements of bacterial cfu and serum cytokines following i.v. infection with l. monocytogenes. at 24 and 48 h postinfection, the brain was ster ... | 2008 | 18566419 |
| systemic listeriosis in caged canaries (serinus canarius). | the occurrence of listeriosis in 12 caged canaries is described where 50% of the birds, including the female and all of the offspring, died within 2 weeks without clinical signs. at necropsy, multifocal necrotizing and partly granulomatous hepatitis, splenitis, myocarditis, interstitial nephritis, and exudative pericarditis with intra-lesional listeria monocytogenes were the predominant findings as shown by histopathology and immunohistochemistry. microbiology, serology and polymerase chain reac ... | 2008 | 18568661 |
| flavonoid treatment of murine macrophages does not alter ingestion or intracellular survival of listeria monocytogenes. | it was recently reported that the aryl hydrocarbon receptor (ahr) plays a role in innate immunity to listeria monocytogenes infection in mice. in this study the authors analyzed whether incubation of murine macrophages with various flavonoids that have agonist or antagonist activity for the ahr (beta-naphthoflavone, alpha-naphthoflavone, quercetin, luteolin) affect the uptake and survival of l. monocytogenes. using the raw264.7 and j774 murine macrophage cell lines and bone marrow-derived macrop ... | 2008 | 18569079 |
| microarray evaluation of the listeria monocytogenes infection and amoxicillin treatment in mice. | by using affymetrix mouse genome arrays and 20 biological replicates per experimental condition, the predictive value of liver and blood gene expression profiles previously identified was validated as predictive of listeria monocytogenes infection severity (lethal and nonlethal infection). the ability of these genes to predict the outcome of antibiotic treatment was also assessed. lethally infected balb/c mice were treated with amoxicillin at 10 or 20 mg/kg; only the higher dose prevented death. ... | 2008 | 18569167 |
| staphylococcus saprophyticus atcc 15305 is internalized into human urinary bladder carcinoma cell line 5637. | invasion of bacteria into nonphagocytic host cells is an important pathogenicity factor for escaping the host defence system. gram-positive organisms, for example staphylococcus aureus and listeria monocytogenes, are invasive in nonphagocytic cells, and this mechanism is discussed as an important part of the infection process. uropathogenic escherichia coli and staphylococcus saprophyticus can cause acute and recurrent urinary tract infections as well as bloodstream infections. staphylococcus sa ... | 2008 | 18573154 |
| safety improvement and preservation of typical sensory qualities of traditional dry fermented sausages using autochthonous starter cultures. | traditional dry fermented sausages are manufactured without addition of starter cultures in small-scale processing units, their fermentation relying on indigenous microflora. characterisation and control of these specific bacteria are essential for the sensory quality and the safety of the sausages. the aim of this study was to develop an autochthonous starter culture that improves safety while preserving the typical sensory characteristics of traditional sausages. an autochthonous starter compo ... | 2008 | 18573561 |
| decreased susceptibility of mice to infection with listeria monocytogenes in the absence of interleukin-18. | the induction of proinflammatory cytokines such as gamma interferon (ifn-gamma) and tumor necrosis factor alpha is crucial for the early control of bacterial infections. since interleukin-18 (il-18) acts as a potent inducer of ifn-gamma, it might play an important role in the induction of a protective immune response in listeriosis. we used a murine model of systemic listeria monocytogenes infection to study the immune response to these intracellular bacteria in the absence of il-18. for this pu ... | 2008 | 18573894 |
| diverse myeloid and lymphoid cell subpopulations produce gamma interferon during early innate immune responses to francisella tularensis live vaccine strain. | francisella tularensis, a small gram-negative intracellular bacterium responsible for causing tularemia, is highly pathogenic and classified as a category a agent of bioterrorism. as for other intracellular pathogens, successful protective immune responses to francisella tularensis require rapid and efficient induction of gamma interferon (ifn-gamma) production. studies using intracellular bacteria such as listeria monocytogenes as well as francisella suggest that natural killer (nk) and t cells ... | 2008 | 18573901 |
| rapid eradication of listeria monocytogenes by moxifloxacin in a murine model of central nervous system listeriosis. | listeriosis is a rare but life-threatening infection. a favorable outcome is greatly aided by early administration of antibiotics with rapid bactericidal activity against listeria monocytogenes. moxifloxacin, a new-generation fluoroquinolone with extended activity against gram-positive bacteria, has proved its effectiveness in vitro against intracellular reservoirs of bacteria. the efficacies of moxifloxacin and amoxicillin were compared in vivo by survival curve assays and by studying the kinet ... | 2008 | 18573932 |
| cooperation between prokaryotic (lde) and eukaryotic (mrp) efflux transporters in j774 macrophages infected with listeria monocytogenes: studies with ciprofloxacin and moxifloxacin. | antibiotic efflux is observed in both eukaryotic and prokaryotic cells, modulating accumulation and resistance. the present study examines whether eukaryotic and prokaryotic fluoroquinolone transporters can cooperate in the context of an intracellular infection. we have used (i) j774 macrophages (comparing a ciprofloxacin-resistant cell line overexpressing an mrp-like transporter with wild-type cells with basal expression), (ii) listeria monocytogenes (comparing a clinical isolate [clip21369] di ... | 2008 | 18573933 |
| antimicrobial activity of dc-159a, a new fluoroquinolone, against 1,149 recently collected clinical isolates. | the activity of dc-159a, a novel orally administered fluorinated quinolone, was evaluated by reference broth microdilution or agar dilution methods against 1,149 recently collected clinical isolates from five continents. against pathogens associated with community-acquired respiratory tract infections (ca-rtis), the mic(90)s were 0.12 microg/ml for streptococcus pneumoniae, 0.015 to 0.03 microg/ml for haemophilus influenzae, 0.03 microg/ml for moraxella catarrhalis, and 0.12 microg/ml for beta-h ... | 2008 | 18573936 |
| single-chain variable fragment antibodies selected by phage display against the sporozoite surface antigen p23 of cryptosporidium parvum. | cryptosporidium parvum, an apicomplexan parasite transmitted via animal fecal wastes, is the causative agent of cryptosporidiosis. clones were selected from 2 synthetic naïve human single-chain variable fragment (scfv) phagemid libraries that bound to the recombinant p23 protein of c. parvum. panning the tomlinson i and j phagemid libraries resulted in 6 distinct clones. two clones had full-length scfv sequences, while the remaining clones were either truncated or missing a section of the heavy ... | 2009 | 18576885 |
| growth kinetics of listeria monocytogenes in broth and beef frankfurters--determination of lag phase duration and exponential growth rate under isothermal conditions. | the objective of this study was to develop a new kinetic model to describe the isothermal growth of microorganisms. the new model was tested with listeria monocytogenes in tryptic soy broth and frankfurters, and compared with 2 commonly used models-baranyi and modified gompertz models. bias factor (bf), accuracy factor (af), and root mean square errors (rmse) were used to evaluate the 3 models. either in broth or in frankfurter samples, there were no significant differences in bf (approximately ... | 2008 | 18576996 |
| edible coatings for enhancing microbial safety and extending shelf life of hard-boiled eggs. | hard-boiled eggs were coated with chitosan-lysozyme (cl), whey protein isolate (wpi), or bake sheen (bs), inoculated with listeria monocytogenes or salmonella enterica ser. enteritidis at 10(4) cfu/g, and stored for 4 wk at 10 degrees c. microbial populations were enumerated weekly. two nonchallenge studies were also conducted with hard-boiled eggs: coated shell-on eggs packaged in plastic containers or peeled eggs coated and vacuum-packaged. total plate counts (tpc), coliforms, yeasts, molds, w ... | 2008 | 18577005 |
| control of listeria monocytogenes in ready-to-eat meats containing sodium levulinate, sodium lactate, or a combination of sodium lactate and sodium diacetate. | this study investigated the use of sodium levulinate to prevent outgrowth of listeria monocytogenes in refrigerated ready-to-eat (rte) meat products. turkey breast roll and bologna were formulated to contain 1%, 2%, or 3% (w/w) sodium levulinate, 2% sodium lactate, a 2% combination of sodium lactate and sodium diacetate (1.875% sodium lactate and 0.125% sodium diacetate), or no antimicrobial (control). samples of the rte products were sliced, inoculated with 10(2) to 10(3) cfu/cm(2) of a 5-strai ... | 2008 | 18577007 |
| genomic and proteomic analysis of the alkali-tolerance response (altr) in listeria monocytogenes 10403s. | information regarding the alkali-tolerance response (altr) in listeria monocytogenes is very limited. treatment of alkali-adapted cells with the protein synthesis inhibitor chloramphenicol has revealed that the altr is at least partially protein-dependent. in order to gain a more comprehensive perspective on the physiology and regulation of the altr, we compared differential gene expression and protein content of cells adapted at ph 9.5 and un-adapted cells (ph 7.0) using complementary dna (cdna ... | 2008 | 18577215 |
| close packing of listeria monocytogenes acta, a natively unfolded protein, enhances f-actin assembly without dimerization. | studies of the biochemistry of listeria monocytogenes virulence protein acta have typically focused on the behavior of bacteria in complex systems or on the characterization of the protein after expression and purification. although prior in vivo work has proposed that acta forms dimers on the surface of l. monocytogenes, dimerization has not been demonstrated in vitro, and little consideration has been given to the surface environment where acta performs its pivotal role in bacterial actin-base ... | 2008 | 18577520 |
| the effect of an acidic, copper sulfate-based commercial sanitizer on indicator, pathogenic, and spoilage bacteria associated with broiler chicken carcasses when applied at various intervention points during poultry processing. | studies were conducted to evaluate 1) the effect of an acidic, copper sulfate-based commercial sanitizer on pathogenic, indicator, and spoilage bacteria in a model scalder system, 2) the effect of this sanitizer on total aerobic bacteria (apc) and escherichia coli counts, and salmonella prevalence on broiler chicken carcasses when applied during scalding or scalding and postpick dipping, and 3) the ability of sanitizer to extend the shelf-life of broiler chicken carcasses. exposure of salmonella ... | 2008 | 18577627 |
| [neurological complications in renal transplant recipients]. | renal transplantation is method of choice for treatment of patients with end-stage renal disease without contraindications for immunosuppressive therapy. neurological complications occur frequently in renal transplant recipients. they may be the consequence of immunosuppressive treatment, but more often evolve as the consequence of previous disturbances which developed during the state of uraemia and treatment with dialysis. the most pronounced neurotoxic effect has calcineurin inhibitors tacrol ... | 2008 | 18578336 |
| serum procalcitonin for discrimination between septic and non-septic arthritis. | early differentiation between septic and non-septic arthritis is difficult. a previous study showed promising diagnostic accuracy of serum procalcitonin (pct) in septic arthritis, limited by a low sensitive pct test kit. | 2008 | 18578968 |
| efns guideline on the management of community-acquired bacterial meningitis: report of an efns task force on acute bacterial meningitis in older children and adults. | acute bacterial meningitis (abm) is a potentially life-threatening neurological emergency. an agreed protocol for early, evidence-based and effective management of community-acquired abm is essential for best possible outcome. a literature search of peer-reviewed articles on abm was used to collect data on the management of abm in older children and adults. based on the strength of published evidence, a consensus guideline was developed for initial management, investigations, antibiotics and sup ... | 2008 | 18582342 |
| the dead-box helicase ddx3x is a critical component of the tank-binding kinase 1-dependent innate immune response. | tank-binding kinase 1 (tbk1) is of central importance for the induction of type-i interferon (ifn) in response to pathogens. we identified the dead-box helicase ddx3x as an interaction partner of tbk1. tbk1 and ddx3x acted synergistically in their ability to stimulate the ifn promoter, whereas rnai-mediated reduction of ddx3x expression led to an impairment of ifn production. chromatin immunoprecipitation indicated that ddx3x is recruited to the ifn promoter upon infection with listeria monocyto ... | 2008 | 18583960 |
| pcr-based detection in a micro-fabricated platform. | we present a novel, on-chip system for the electrokinetic capture of bacterial cells and their identification using the polymerase chain reaction (pcr). the system comprises a glass-silicon platform with a set of micro-channels, -chambers, and -electrodes. a platinum thin film resistor, placed in the proximity of the chambers, is used for temperature monitoring. the whole chip assembly is mounted on a printed circuit board (pcb) and wire-bonded to it. the pcb has an embedded heater that is utili ... | 2008 | 18584089 |
| anti-infective mode of action of eps 7630 at the molecular level. | clinical trials have shown that eps 7630, an aqueous ethanolic extract from the roots of pelargonium sidoides, is an efficacious treatment for respiratory tract infections. a large body of in vitro studies has provided evidence for an anti-infective principle associated with activation of the non-specific immune system. however, the mode of action at the cellular and molecular level is still insufficiently defined. this study, therefore, aimed to provide further insight into the underlying princ ... | 2008 | 18584813 |
| loss of flagellum-based motility by listeria monocytogenes results in formation of hyperbiofilms. | biofilm formation by the gram-positive, motile, food-borne pathogen listeria monocytogenes was demonstrated to occur by an ordered series of stages. biofilm development involves flagellum-based motility, which when blocked decreases initial bacterial surface attachment but subsequently leads to the formation of hyperbiofilms, surface-attached communities reaching high density. | 2008 | 18586947 |
| differences in survival among 13 listeria monocytogenes strains in a dynamic model of the stomach and small intestine. | thirteen listeria monocytogenes strains were compared for the ability to survive in a dynamic gastrointestinal model. strains displayed various degrees of susceptibility to gastric acidity; however, strain-to-strain variations became evident mainly after 90 min of exposure (ph 2.0). cell levels transferred to the intestine depended on initial populations, while reductions during intestinal exposure were relatively small for all strains. | 2008 | 18586963 |
| [analysis of antibiotic susceptibility of foodborne listeria monocytogenes in china]. | to study the antibiotic susceptibility of foodborne listeria monocytogenes in china. | 2008 | 18589603 |
| acid and nacl limits to growth of listeria monocytogenes and influence of sequence of inimical acid and nacl levels on inactivation kinetics. | variability in growth limits of listeria monocytogenes in response to low ph (adjusted with hcl) or high salinity (nacl) was evaluated for 127 strains in brain heart infusion broth at 25 degrees c. over 95% of strains habituated at ph 5.0 grew subsequently at ph 4.2, while 25% were able to grow at ph 4.1. more than 85% of strains preadapted to growth at 8.5% nacl (wt/vol) subsequently grew in the presence of 11.3% nacl, while 25% were able to grow at 13% nacl, and 4.7% grew in the presence of 13 ... | 2008 | 18592742 |
| identification and characterization of enterococcus spp. in greek spontaneous sausage fermentation. | a total of 108 enterococcal strains previously isolated from spontaneously fermented sausages were identified using phenotypic traits, pulsed-field gel electrophoresis (pfge), and sequencing of the 16s rrna gene. the proteolytic and lipolytic activities of these isolates and their ability to decarboxylate lysine, tyrosine, ornithine, and histidine and to produce antimicrobial compounds also were assessed. all strains were identified as enterococcus faecium, and a lack of correlation between data ... | 2008 | 18592753 |
| role of the u.s. food and drug administration in the regulatory management of human listeriosis in the united states. | from 1986 to 2006, the incidence of listeriosis in the united states dropped from approximately seven to three cases per million population, a reduction that most likely reflects the joint efforts of industry, government, consumers, and academia. herein, we describe the u.s. food and drug administration (fda) strategy over the past three decades to combat listeriosis. specifically, we discuss early actions taken to address outbreaks during the 1980s, policy decisions regarding the presence of li ... | 2008 | 18592761 |
| incidence and growth of listeria monocytogenes in persimmon (diospyros kaki) fruit. | the incidence of listeria monocytogenes on persimmon (diospyros kaki) surface of 'fuyu' and 'rama forte' was evaluated during a 5-month-period (from march to july) of two seasons periods (years 2005 and 2006). the fruits were collected in wholesale and street markets and retail in sao paulo and campinas city, brazil. a total of 582 fruits were analyzed using the bax system which is based on the polymerase chain reaction. the ability of this pathogen to grow on the peel and pulp of the two persim ... | 2008 | 18599141 |
| a novel cell wall-anchored peptidoglycan hydrolase (autolysin), ispc, essential for listeria monocytogenes virulence: genetic and proteomic analysis. | we have recently concluded that a listeria monocytogenes 86 kda immunogenic surface protein, ispc, is a cell wall-anchored peptidoglycan hydrolase (autolysin), capable of degrading the cell wall peptidoglycan of the bacterium itself. to determine if this enzyme has any biological functions and/or plays a role in virulence, we in-frame-deleted the ispc gene from the l. monocytogenes chromosome. this deltaispc mutant exhibited complete abrogation of expression of ispc and displayed no defects in i ... | 2008 | 18599819 |
| point mutations within the streptococcal regulator of virulence (srv) alter protein-dna interactions and srv function. | group a streptococcus (gas) possesses a complex regulatory system enabling the organism to colonize a range of physiologically distinct host sites. within this network of regulators is the streptococcal regulator of virulence (srv). srv is a member of the crp/fnr family of transcriptional regulators and is most similar to pleiotropic regulatory factor a (prfa), a positive regulator of virulence in listeria monocytogenes. members of this family possess a characteristic c-terminal helix-turn-helix ... | 2008 | 18599828 |
| risk profiles of pork and poultry meat and risk ratings of various pathogen/product combinations. | risk profiles of pork and poultry meat were carried out using an excel-based software program, risk ranger. it is a semi-quantitative risk estimator answering various questions relating to the probability of exposure to a hazard, susceptibility of the population of interest, severity of the illness caused by the hazard if present and probability of food containing an infectious dose. therefore, qualitative and quantitative inputs were used to estimate and rank the risk of various hazards/food co ... | 2008 | 18602180 |
| the evaluation of the immunomodulating properties of era-63 a pharmaceutical with estrogenic activity. | this paper describes studies performed with era-63 a low molecular weight pharmaceutical with intended immunomodulatory effects. since this compound was also known to have estrogenic activity a non-conventional approach was taken in order to differentiate between estrogenic and non-estrogenic-induced immunomodulatory effects. ee was included not only for qualitative comparison (hazard identification) between immunomodulatory effects but also, in case of similar effects, to facilitate the extrapo ... | 2008 | 18602456 |
| autophagic control of listeria through intracellular innate immune recognition in drosophila. | autophagy, an evolutionally conserved homeostatic process for catabolizing cytoplasmic components, has been linked to the elimination of intracellular pathogens during mammalian innate immune responses. however, the mechanisms underlying cytoplasmic infection-induced autophagy and the function of autophagy in host survival after infection with intracellular pathogens remain unknown. here we report that in drosophila, recognition of diaminopimelic acid-type peptidoglycan by the pattern-recognitio ... | 2008 | 18604211 |
| inactivation of barotolerant strains of listeria monocytogenes and escherichia coli o157:h7 by ultra high pressure and tert-butylhydroquinone combination. | antimicrobial efficacy of ultra-high-pressure (uhp) can be enhanced by application of additional hurdles. the objective of this study was to systematically assess the enhancement in pressure lethality by tbhq treatment, against barotolerant strains of escherichia coli o157:h7 and listeria monocytogenes. two l. monocytogenes scott a and the barotolerant osy-328 strain, and two e. coli o157:h7 strains, edl-933 and its barotolerant mutant, osy-asm, were tested. cell suspensions containing tbhq (50 ... | 2008 | 18604498 |
| intracellular bacteriolysis triggers a massive apoptotic cell death in shigella-infected epithelial cells. | infected epithelial cells, which act as a first barrier against pathogens, seldom undergo apoptosis. rather, infected epithelial cells undergo a slow cell death that displays hallmarks of necrosis. here, we demonstrate that rapid intracellular lysis of shigella flexneri, provoked by either the use of a diaminopimelic acid auxotroph mutant or treatment of infected cells with antibiotics of the beta-lactam family, resulted in a massive and rapid induction of apoptotic cell death. this intracellula ... | 2008 | 18606244 |
| granzymes drive a rapid listeriolysin o-induced t cell apoptosis. | the listeria monocytogenes protein listeriolysin o (llo) is a pore-forming protein essential for virulence. although the major role for llo is to allow l. monocytogenes entry into the cytosol, it also induces apoptosis of activated lymphocytes, an obligatory cellular response that modulates the infection. induction of apoptosis by llo proceeds through a fast, caspase-dependent pathway and a slow, caspase-independent pathway. polyclonal t cell lines were generated from either normal mice or mice ... | 2008 | 18606691 |
| identification of formyl peptides from listeria monocytogenes and staphylococcus aureus as potent chemoattractants for mouse neutrophils. | the prototypic formyl peptide n-formyl-met-leu-phe (fmlf) is a major chemoattractant found in escherichia coli culture supernatants and a potent agonist at human formyl peptide receptor (fpr) 1. consistent with this, fmlf induces bactericidal functions in human neutrophils at nanomolar concentrations. however, it is a much less potent agonist for mouse fpr (mfpr) 1 and mouse neutrophils, requiring micromolar concentrations for cell activation. to determine whether other bacteria produce more pot ... | 2008 | 18606697 |
| rapid identification and typing of listeria species by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | listeria monocytogenes is a food-borne pathogen that is the causative agent of human listeriosis, an opportunistic infection that primarily infects pregnant women and immunologically compromised individuals. rapid, accurate discrimination between listeria strains is essential for appropriate therapeutic management and timely intervention for infection control. a rapid method involving matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) that shows promise f ... | 2008 | 18606788 |
| subinhibitory concentrations of beta-lactams and other cell-wall antibiotics inhibit listeriolysin production by listeria monocytogenes. | in the present study we tested the ability of beta-lactam antibiotics and other cell-wall antibiotics to inhibit the production of listeriolysin which is the main virulence factor of l. monocytogenes. the amount of listeriolysin produced was measured in the supernatants with a sensitive hemolysin assay and as beta-galactosidase expression from the listeriolysin promoter. when tested in concentrations that did not affect growth of the bacteria, all beta-lactam antibiotics and all other cell-wall ... | 1996 | 18611740 |
| the protein tyrosine phosphatase ptpn4/ptp-meg1, an enzyme capable of dephosphorylating the tcr itams and regulating nf-kappab, is dispensable for t cell development and/or t cell effector functions. | t cell receptor signaling processes are controlled by the integrated actions of families of protein tyrosine kinases (ptks) and protein tyrosine phosphatases (ptpases). several distinct cytosolic protein tyrosine phosphatases have been described that are able to negatively regulate tcr signaling pathways, including shp-1, shp-2, ptph1, and pep. using ptpase substrate-trapping mutants and wild type enzymes, we determined that ptpn4/ptp-meg1, a ptph1-family member, could complex and dephosphorylat ... | 2008 | 18614237 |
| genetic variation of listeria monocytogenes isolates from domestic and imported foods in japan. | phylogenetic analyses were carried out on a total of 118 listeria monocytogenes isolates from foods or food processing environments, and 7 isolates from listeriosis patients in japan to evaluate the genetic variation in the pathogen in this country. isolates of serotypes 1/2a, 1/2b and 4b were mainly examined to assess the risk of exposure of humans to l. monocytogenes from foods in japan. the nucleotide sequences of the part of the iap gene that contains the region encoding the threonine-aspara ... | 2008 | 18614253 |
| children with bacterial meningitis presenting to the emergency department during the pneumococcal conjugate vaccine era. | the epidemiology of bacterial meningitis in children in the era of widespread heptavalent conjugate pneumococcal vaccination (pcv7) is unknown. | 2008 | 18616437 |
| echinacea-induced macrophage activation. | public interest in echinacea is growing rapidly. unfortunately, there is little scientific evidence to support claims of efficacy of this widely used botanical, and little information about potential mechanism of action. this study examines the ability of echinacea to upregulate macrophage function and begins to elucidate the mechanism of echinacea-induced macrophage activation. murine peritoneal macrophages were cultured with e. purpurea extracts enriched for plant polysaccharide (ep). elisa wa ... | 2008 | 18618312 |
| listeria monocytogenes septicaemia and concurrent clostridial infection in an adult alpaca (lama pacos). | a 10-year-old alpaca with a history of anorexia, weight loss and diarrhoea was humanely destroyed and shown to have a multifocal necrotizing hepatitis, splenitis and colitis, as well as an ulcerative to diphtheroid ileitis. immunohistochemical examination revealed listeria monocytogenes antigen in the liver and ileum. in addition, l. monocytogenes and listeria sp.-specific gene fragments were detected by the polymerase chain reaction. l. monocytogenes was isolated from liver and small intestine ... | 2008 | 18619608 |
| modulation of stress and virulence in listeria monocytogenes. | listeria monocytogenes can respond rapidly to changing environmental conditions, as illustrated by its ability to transition from a saprophyte to an orally transmitted facultative intracellular pathogen. differential associations between various alternative sigma factors and a core rna polymerase provide a transcriptional mechanism for regulating bacterial gene expression that is crucial for survival in rapidly changing conditions. alternative sigma factors are key components of complex l. monoc ... | 2008 | 18619843 |
| bacteriocin-producing lactobacillus strains isolated from poto poto, a congolese fermented maize product, and genetic fingerprinting of their plantaricin operons. | thirty one bacteriocin-producing lactobacillus isolates were identified among 135 lactobacilli isolated from the congolese fermented maize product poto poto, during the preparation and from the finished product. using species-specific pcr and 16s rrna gene sequencing, 28 and 3 isolates were identified as l. plantarum and l. fermentum, respectively. cluster analysis of rapd-pcr fingerprints revealed two main groups (g1 and g2) plus the l. fermentum isolate c4-13. group g1 contained 23 isolates wi ... | 2008 | 18620772 |
| viability of multi-strain mixtures of listeria monocytogenes, salmonella typhimurium, or escherichia coli o157:h7 inoculated into the batter or onto the surface of a soudjouk-style fermented semi-dry sausage. | the fate of listeria monocytogenes, salmonella typhimurium, or escherichia coli o157:h7 were separately monitored both in and on soudjouk. fermentation and drying alone reduced numbers of l. monocytogenes by 0.07 and 0.74 log(10)cfu/g for sausages fermented to ph 5.3 and 4.8, respectively, whereas numbers of s. typhimurium and e. coli o157:h7 were reduced by 1.52 and 3.51 log(10)cfu/g and 0.03 and 1.11 log(10)cfu/g, respectively. when sausages fermented to ph 5.3 or 4.8 were stored at 4, 10, or ... | 2008 | 18620971 |
| the response of hek293 cells transfected with bovine tlr2 to established pathogen-associated molecular patterns and to bacteria causing mastitis in cattle. | toll-like receptors (tlrs) are key sensors of pathogen-associated molecular patterns (pamps). their role in immunity is difficult to examine in species of veterinary interest, due to restricted access to the knockout technology and tlr-specific antibodies. an alternative approach is to generate cell lines transfected with various tlrs and to examine the recognition of pamps or relevant bacteria. in this report, we examined whether recognition of various pamps and mastitis-causing bacteria is ach ... | 2008 | 18621422 |
| identification of a sigma b-dependent small noncoding rna in listeria monocytogenes. | in listeria monocytogenes, the alternative sigma factor sigma(b) plays important roles in stress tolerance and virulence. here, we present the identification of sbra, a novel small noncoding rna that is produced in a sigma(b)-dependent manner. this finding adds the sigma(b) regulon to the growing list of stress-induced regulatory circuits that include small noncoding rnas. | 2008 | 18621897 |
| successive post-translational modifications of e-cadherin are required for inla-mediated internalization of listeria monocytogenes. | listeria monocytogenes surface proteins internalin (inl)a and inlb interact with the junctional protein e-cadherin and the hepatocyte growth factor (hgf) receptor met, respectively, on the surface of epithelial cells to mediate bacterial entry. here we show that inla triggers two successive e-cadherin post-translational modifications, i.e. the src-mediated tyrosine phosphorylation of e-cadherin followed by its ubiquitination by the ubiquitin-ligase hakai. e-cadherin ubiquitination induces the re ... | 2008 | 18624796 |
| bioprotective leuconostoc strains against listeria monocytogenes in fresh fruits and vegetables. | ten leuconostoc mesenteroides and one ln. citreum strains isolated from fresh fruit and vegetables were tested for their antagonistic capacity against listeria monocytogenes. genetic differences among strains were analyzed by random amplified polymorphic dna (rapd). all the isolates clustered together and differed from the type strain ln. mesenteroides atcc 8293 as well as from ln. fallax and ln. citreum. organic acids, hydrogen peroxide and bacteriocins were detected as main inhibition mechanis ... | 2008 | 18625532 |
| carbon metabolism of listeria monocytogenes growing inside macrophages. | the intracellular metabolism of listeria monocytogenes was studied by (13)c-isotopologue profiling using murine j774a.1 macrophages as host cells. six hours after infection, bacteria were separated from the macrophages and hydrolyzed. amino acids were converted into tert-butyl-dimethylsilyl derivatives and subjected to gas chromatography/mass spectrometry. when the macrophages were supplied with [u-(13)c(6)]glucose prior to infection, but not during infection, label was detected only in ala, asp ... | 2008 | 18627458 |
| ribdb: an srs based infrastructure for realis. | the realis project is an eu-funded consortium for the post genomic analysis of the food pathogen listeria monocytogenes. the data generated by the consortium members is stored under the ribdb database, a system built using srs which integrates consortium data, public databases, and applications for analysis. ribdb is available to all consortium members through a web server, with the option of installing a local mirror of the main server for local analysis. | 2002 | 18628878 |
| realis: postgenomic analysis of listeria monocytogenes. | listeria monocytogenes is a remarkably successful food-borne pathogen. it is capable a) of surviving and proliferating under conditions that exist within the food chain, such as at low temperatures, high salt and low ph and b) of colonizing animal host tissues after ingestion of contaminated food, causing opportunistic infections mainly, but not exclusively, in immunocompromised hosts. the ultimate goals of realis are two fold: firstly, it aims to completely decipher all genes required for survi ... | 2002 | 18628879 |
| listeria monocytogenes multidrug resistance transporters activate a cytosolic surveillance pathway of innate immunity. | to gain insight into the interaction of intracellular pathogens with host innate immune pathways, we performed an unbiased genetic screen of listeria monocytogenes mutants that induced an enhanced or diminished host innate immune response. here, we show that the major facilitator superfamily of bacterial multidrug resistance transporters (mdrs) controlled the magnitude of a host cytosolic surveillance pathway, leading to the production of several cytokines, including type i ifn. mutations mappin ... | 2008 | 18632558 |
| regional variations in paneth cell antimicrobial peptide expression along the mouse intestinal tract. | enteric antimicrobial peptides secreted from paneth cells, including alpha-defensins (in mice named cryptdins), are key effector molecules of innate immunity in the small intestine. the importance of paneth cells alpha-defensins emerged from studies of enteric bacterial infection in genetically modified mice, as well as from recent studies linking reduced levels of these alpha-defensins to crohn's disease localized to the ileum. however, analysis of expression of paneth cell alpha-defensins is i ... | 2008 | 18637162 |
| ifn-gamma induces the erosion of preexisting cd8 t cell memory during infection with a heterologous intracellular bacterium. | memory t cells are critical for the control of intracellular pathogens and require few signals for maintenance; however, erosion of established preexisting memory cd8(+) t cells has been shown to occur during infection with heterologous viral infections. we evaluated whether this also occurs during infection with various intracellular bacteria and what mechanisms may be involved. we demonstrate that erosion of established memory is also induced during infection of mice with various intracellular ... | 2008 | 18641306 |
| lipoproteins of listeria monocytogenes are critical for virulence and tlr2-mediated immune activation. | numerous cell surface components of listeria influence and regulate innate immune recognition and virulence. here, we demonstrate that lipidation of prelipoproteins in listeria monocytogenes is required to promote nf-kappab activation via tlr2. in hela cells transiently expressing tlr2, l. monocytogenes and listeria innocua mutants lacking the prolipoprotein diacylglyceryl transferase (lgt) gene are unable to induce tlr2-dependent activation of nf-kappab, a property intrinsic to their isogenic p ... | 2008 | 18641340 |
| adhesion of the probiotic strains enterococcus mundtii st4sa and lactobacillus plantarum 423 to caco-2 cells under conditions simulating the intestinal tract, and in the presence of antibiotics and anti-inflammatory medicaments. | adhesion of enterococcus mundtii st4sa and lactobacillus plantarum 423 to caco-2 (human carcinoma epithelial) cells was visualized by fluorescent staining. both strains showed good adhesion compared to l. casei mb1, l. casei shirota, l. johnsonii la1 and l. rhamnosus gg. no correlation was found between hydrophobicity, aggregation and adhesion to caco-2 cells. presence of antibiotics and anti-inflammatory medicaments reduced adhesion of bacterial strains to caco-2 cells. proteins sensitive to pe ... | 2008 | 18641972 |
| the anti-inflammatory drug diclofenac retains anti-listerial activity in vivo. | the interactions between nonsteroidal anti-inflammatory drugs (nsaid) and listeria monocytogenes have not been sufficiently documented to date. the aim of this study was to investigate the possible effects of diclofenac (dc) in a murine listerial infection model. | 2008 | 18643914 |
| a 'fly-by' killing with a primordial cellular weapon. | | 2008 | 18645586 |
| [listeria monocytogenes infection during pregnancy--modern aspects]. | during a ten years period (1997-2006) at the delivery ward of maychin dom university hospital were admitted 31,430 parturients. among all of them we indentified and evaluated only 4 pregnant patients with listeria monocytogenes infection. during the first 8 years of the investigated period there was no even single case of the infection, whilst during the last 2 years the frequency of the listeria infection among all birth deliveries was 0,068%. all of our four cases were admitted with clinical s ... | 2007 | 18646305 |