| attenuated listeria infection activates natural killer cell cytotoxicity to regress melanoma growth in vivo. | listeria monocytogenes infection induces various types of immune responses. the lm-induced immunity not only protects the hosts against lm infection but also has a therapeutic effect on other diseases such as tumors and infectious diseases. in the present study, we sought to identify the cells and molecules that are primarily responsible for the lm-induced antitumor immune response. we investigated the mechanism of the antitumor immune response induced by lm infection using melanoma cells and va ... | 2008 | 18380808 |
| using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia. | the aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in chinese hospitals. the multiplexed array, based on the qiagen liquichip workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. pcr amplicons of a variable region of the bacterial 23s rrna genes were hybridized to the bead-bound probes. thirty-eight strains belonging ... | 2008 | 18381803 |
| testing human biologicals in animal host resistance models. | the purpose of immunotoxicity testing is to obtain data that is meaningful for safety assessment. host resistance assays are the best measure of a toxicant's effect on the overall ability to mount an effective immune response and protect the host from infectious disease. an outline is presented for immunotoxicological evaluation using host resistance assays. the influenza virus host resistance model is useful to evaluate the overall health of the immune system and is one of the most thoroughly c ... | 2008 | 18382855 |
| mechanical properties of a gelidium corneum edible film containing catechin and its application in sausages. | we prepared an edible gelidium corneum (gc) film containing catechin and examined the microbial growth and quality change during storage of sausages packaged with the film. incorporation of catechin in the film improved film tensile strength and water vapor permeability. the film's antimicrobial activity against eschericha coli o157:h7 increased with increasing catechin concentrations and resulted in a decrease in the populations of the bacteria by 1.93 log cfu/g at 150 mg of catechin. for the s ... | 2008 | 18387102 |
| biodegradable polylactic acid polymer with nisin for use in antimicrobial food packaging. | biodegradable polylactic acid (pla) polymer was evaluated for its application as a material for antimicrobial food packaging. pla films were incorporated with nisin to for control of foodborne pathogens. antimicrobial activity of pla/nisin films against listeria monocytogenes, escherichia coli o157:h7, and salmonella enteritidis were evaluated in culture media and liquid foods (orange juice and liquid egg white). scanned electron micrograph and confocal laser microscopy revealed that nisin parti ... | 2008 | 18387115 |
| multi-method approach indicates no presence of sub-lethally injured listeria monocytogenes cells after mild heat treatment. | application of mild inactivation treatments follows an increasing trend in the food industry and is often combined with sub-optimal intrinsic product conditions to ensure appropriate level of microbial safety. listeria monocytogenes was subjected to mild heat treatment (20 min at 60 degrees c) and subsequently exposed to various mild preservation conditions based on increased nacl concentration and decreased ph. recovery and resuscitation of l. monocytogenes cells were studied using various meth ... | 2008 | 18387685 |
| constitutive crosspresentation of tissue antigens by dendritic cells controls cd8+ t cell tolerance in vivo. | immature dendritic cells (dcs) sample tissue-specific antigens (tsas) and process them for "crosspresentation" via major histocompatibility complex (mhc) class i and ii molecules. findings with adoptively transferred t cell receptor (tcr)-transgenic cd8+ t cells in transgenic mice expressing model tsa indicate that this process contributes to tolerance induction of cd8+ t cells, a phenomenon termed "crosstolerance." however, up to now it has been unknown whether "crosstolerance" can also control ... | 2008 | 18387832 |
| effect of inoculum preparation procedure and storage time and temperature on the fate of listeria monocytogenes on inoculated salami. | although dry/semidry fermented sausages are characterized as being of low-to-moderate risk for human listeriosis on a per-serving and per-annum basis, data are lacking relative to the fate of postprocessing listeria monocytogenes contamination during storage of such products. this study evaluated the effect of inoculum preparation and storage conditions on the fate of l. monocytogenes on vacuum-packaged salami. commercially produced salami was sliced and inoculated (4 +/- 1.3 log cfu/ cm2) with ... | 2008 | 18389691 |
| antibacterial efficiency of spanish satureja montana essential oil against listeria monocytogenes among natural flora in minced pork. | the purpose of this study was to examine the effectiveness of winter savory (satureja montana) essential oil (eo) for control of growth and survival of experimentally inoculated listeria monocytogenes serovar 4b (10(4) cfu/g) among natural flora in minced pork. eos of french thyme (thymus vulgaris f) and rosemary (rosmarinus officinalis) cultivated in the same region of aragon (northeastern spain) were used as reference ingredients. the eos obtained by hydrodistillation were added at concentrati ... | 2008 | 18389692 |
| low prevalence of listeria monocytogenes in cull sows and pork. | the goal of this study was to determine the prevalence of listeria monocytogenes in sows slaughtered at a single midwestern plant on two occasions (trial 1, n = 179 sows; trial 2, n = 160 sows). fecal samples collected antemortem (trial 1) as well as animal tissues, and carcass swabs collected at the abattoir (trials 1 and 2) were analyzed. eight isolates of l. monocytogenes were recovered from five samples that represented 0.18% of the total samples (n = 2,775). in trial 1, l. monocytogenes was ... | 2008 | 18389698 |
| effect of acidified sodium chlorite, chlorine, and acidic electrolyzed water on escherichia coli o157:h7, salmonella, and listeria monocytogenes inoculated onto leafy greens. | recent foodborne outbreaks implicating spinach and lettuce have increased consumer concerns regarding the safety of fresh produce. while the most common commercial antimicrobial intervention for fresh produce is wash water containing 50 to 200 ppm chlorine, this study compares the effectiveness of acidified sodium chlorite, chlorine, and acidic electrolyzed water for inactivating escherichia coli o157:h7, salmonella, and listeria monocytogenes inoculated onto leafy greens. fresh mixed greens wer ... | 2008 | 18389712 |
| use of uv light for the inactivation of listeria monocytogenes and lactic acid bacteria species in recirculated chill brines. | ready-to-eat meat products have been implicated in several foodborne listeriosis outbreaks. microbial contamination of these products can occur after thermal processing when products are chilled in salt brines. the objective of this study was to evaluate uv radiation on the inactivation of listeria monocytogenes and lactic acid bacteria in a model brine chiller system. two concentrations of brine (7.9% [wt/wt] or 13.2% [wt/wt]) were inoculated with a approximately 6.0 log cfu/ml cocktail of l. m ... | 2008 | 18389713 |
| influence of peroxyacetic acid and nisin and coculture with enterococcus faecium on listeria monocytogenes biofilm formation. | biofilm formation is a matter of concern in food industries because biofilms facilitate the survival of pathogenic bacteria such as listeria monocytogenes, which may contaminate food-processing equipment and products. in this study, nisin and two enterococcus faecium strains were evaluated for their effect on biofilm formation by l. monocytogenes cultured in brain heart infusion broth and on stainless steel coupons. elimination of preformed l. monocytogenes biofilms by peroxyacetic acid also was ... | 2008 | 18389714 |
| microbial growth and the effects of mild acidification and preservatives in refrigerated sweet potato puree. | refrigerated sweet potato puree is a convenient form of sweet potato that can be used as an ingredient in formulated foods. the microbiology of refrigerated sweet potato puree during storage for up to 5 weeks was evaluated. because the puree was made by comminuting steam-cooked sweet potatoes before refrigeration, no naturally occurring vegetative bacterial cells were detected during a 4-week period of refrigerated storage at 4 degrees c. however, if postprocessing microbial contamination of the ... | 2008 | 18389715 |
| the long isoform of cellular flip is essential for t lymphocyte proliferation through an nf-kappab-independent pathway. | although the long isoform of cellular flip (c-flip(l)) has been implicated in tcr-mediated signaling, its role in t cell proliferation remains controversial. some studies have demonstrated that overexpression of c-flip(l) promotes t cell proliferation and nf-kappab activation, whereas others have reported that c-flip(l) overexpression has no effect or even inhibits t cell proliferation. to establish the role of c-flip(l) in t lymphocyte proliferation, we have generated a conditional knockout mou ... | 2008 | 18390734 |
| protein kinase c-theta critically regulates the proliferation and survival of pathogen-specific t cells in murine listeriosis. | protein kinase c-theta (pkc-theta) is essential for the activation of t cells in autoimmune disorders, but not in viral infections. to study the role of pkc-theta in bacterial infections, pkc-theta(-/-) and wild-type mice were infected with listeria monocytogenes (lm). in primary and secondary listeriosis, the numbers of lm-specific cd8 and cd4 t cells were drastically reduced in pkc-theta(-/-) mice, resulting in increased cfus in spleen and liver of both pkc-theta(-/-) c57bl/6 and balb/c mice. ... | 2008 | 18390745 |
| acquisition of the vacuolar atpase proton pump and phagosome acidification are essential for escape of francisella tularensis into the macrophage cytosol. | the francisella tularensis-containing phagosome (fcp) matures to a late-endosome-like phagosome prior to bacterial escape into the cytosols of macrophages, where bacterial proliferation occurs. our data show that within the first 15 min after infection of primary human monocyte-derived macrophages (hmdms), approximately 90% of the fcps acquire the proton vacuolar atpase (vatpase) pump and the lysomotropic dye lysotracker, which concentrates in acidic compartments, similar to phagosomes harboring ... | 2008 | 18390995 |
| blood monocytes: distinct subsets, how they relate to dendritic cells, and their possible roles in the regulation of t-cell responses. | monocytes can have important effects on the polarization and expansion of lymphocytes and may contribute to shaping primary and memory t-cell responses in humans and mice. however, their precise contribution in terms of cellular subsets and the molecular mechanisms involved remains to be determined. mouse monocytes originate from a bone marrow progenitor, the macrophage and dc precursor (mdp), which also gives rise to conventional dendritic cells through a separate differentiation pathway. mouse ... | 2008 | 18392044 |
| processing plant persistent strains of listeria monocytogenes appear to have a lower virulence potential than clinical strains in selected virulence models. | listeria monocytogenes is an important foodborne bacterial pathogen that can colonize food processing equipment. one group of genetically similar l. monocytogenes strains (rapd type 9) was recently shown to reside in several independent fish processing plants. persistent strains are likely to contaminate food products, and it is important to determine their virulence potential to evaluate risk to consumers. we compared the behaviour of food processing persistent and clinical l. monocytogenes str ... | 2008 | 18394737 |
| listeria comet tails: the actin-based motility machinery at work. | listeria monocytogenes is a master of mimicry that uses the host cell actin system both to move within the cytoplasm of infected cells and for cell-to-cell spread. recent studies of listeria and similarly acting pathogens have generated leaps in our understanding of the actin-based force producing machinery. this machinery is essential for most motile properties of cells, not least for cell migration. in a minimal configuration, it consists of the arp2/3-complex, ena-vasp proteins, cofilin, capp ... | 2008 | 18396046 |
| importance of murine vdelta1gammadelta t cells expressing interferon-gamma and interleukin-17a in innate protection against listeria monocytogenes infection. | murine gammadelta t cells participate in the innate immune response against infection by an intracellular pathogen listeria monocytogenes. vdelta1+gammadelta t cells coexpressing vgamma6 are a major gammadelta t-cell subpopulation induced at an early stage of l. monocytogenes infection in the livers of infected mice. to investigate the protective role of the vgamma6/vdelta1+gammadelta t cells against l. monocytogenes infection, vdelta1 gene-deficient (vdelta1-/-) mice were analysed because these ... | 2008 | 18397272 |
| interactions of some common pathogenic bacteria with acanthamoeba polyphaga. | protozoan grazing is a major trophic pathway whereby the biomass re-enters the food web. nonetheless, not all bacteria are digested by protozoa and the number known to evade digestion, resulting in their environmental augmentation, is increasing. we investigated the interactions of bacillus cereus, enterococcus faecalis, enteropathogenic escherichia coli (epec), listeria monocytogenes, salmonella enterica serovar typhimurium, and methicillin-sensitive staphylococcus aureus (mssa), with the amoeb ... | 2008 | 18399997 |
| prodrug converting enzyme gene delivery by l. monocytogenes. | listeria monocytogenes is a highly versatile bacterial carrier system for introducing protein, dna and rna into mammalian cells. the delivery of tumor antigens with the help of this carrier into tumor-bearing animals has been successfully carried out previously and it was recently reported that l. monocytogenes is able to colonize and replicate within solid tumors after local or even systemic injection. | 2008 | 18402662 |
| effect of water activity on inactivation of listeria monocytogenes and lactate dehydrogenase during high pressure processing. | the aim of this study was to investigate the effect of water activity (aw) on the inactivation of listeria monocytogenes and lactate dehydrogenase (ldh) during high pressure processing (hpp). for microbial inactivation lyophilized cells of l. monocytogenes 19,115 were left dry or were suspended in 10 ml of 0.1% peptone water, 10 ml of glycerol, or mixtures of glycerol and peptone water. all samples of various aws were high pressure (hp) processed at ambient temperature at 600 mpa for 300 s. foll ... | 2008 | 18403036 |
| effective t-cell immune responses in the absence of the serine/threonine kinase rip2. | the serine/threonine kinase rip2 has been reported to be essential for nod1 and nod2 mediated cell activation, and has been suggested to play a role in the signaling cascade downstream of the t-cell receptor. we sought to ascertain the exact role of rip2 in t-helper cell differentiation and cd8+ t-cell effector function in vivo and in vitro. in contrast to previous reports, we found that rip2-deficient t cells did not exhibit impaired proliferation upon tcr engagement in vitro, and differentiati ... | 2008 | 18403232 |
| lipopolysaccharide sensing an important factor in the innate immune response to gram-negative bacterial infections: benefits and hazards of lps hypersensitivity. | in this review, we summarize our investigations concerning the differential importance of cd14 and lbp in toll-like receptor 4 (tlr4)/myeloid differentiation protein-2 (md-2)-mediated signaling by smooth and rough-form lipopolysaccharide (lps) chemotypes and include the results obtained in studies with murine and human tlr4-transgenic mice. furthermore, we present more recent data on the mechanisms involved in the induction of lps hypersensitivity by bacterial and viral infections and on the rea ... | 2008 | 18406367 |
| fusogenicity of membranes: the impact of acid sphingomyelinase on innate immune responses. | acid sphingomyelinase (asmase) has been implemented in cellular signaling mainly because its reaction product, ceramide, has been assumed to be a mediator within signaling pathways. our studies of three independent infection systems show that asmase is required for phago-lysosomal fusion in macrophages infected with listeria monocytogenes, for exocytosis of secretory lysosomes by lymphocytic choriomeningitis virus-specific cytotoxic t cells, and for generation of multinucleated giant cells in gr ... | 2008 | 18406376 |
| immediate lymphotoxin beta receptor-mediated transcriptional response in host defense against l. monocytogenes. | the lymphotoxin beta receptor (ltbetar) mediates crucial signals in host defense against intracellular bacteria and viruses. mice deficient in ltbetar readily succumb to infections with listeria monocytogenes, mycobacterium tuberculosis and murine cytomegalovirus (mcmv). ltbetar has been shown to be important for the early induction of interferon (ifn) beta after infection with mcmv. however, up to now, it is not known which host effector molecules are induced in cells of the innate immune syste ... | 2008 | 18406380 |
| modelling the effect of the redox potential and ph of heating media on listeria monocytogenes heat resistance. | study the effect of redox potential and ph of the heating media on listeria monocytogenes heat resistance and model its action at fixed temperature. | 2008 | 18410341 |
| investigation into the effect of detergents on disinfectant susceptibility of attached escherichia coli and listeria monocytogenes. | investigate the effect of detergent treatment on susceptibility of attached escherichia coli and listeria monocytogenes to subsequent disinfectant treatment. | 2008 | 18410344 |
| epstein-barr virus-induced gene 3 negatively regulates il-17, il-22 and rorgamma t. | epstein-barr virus-induced gene 3 (ebi3) associates with p28 to form il-27 and with il-12p35 to form il-35. il-27ralpha(-/-) mice studies indicate that il-27 negatively regulates th17 cell differentiation. however, no ebi3, p28 or p35-deficiency studies that directly address the role of ebi3, p28 or p35 on th17 cells have been done. here, we demonstrate that spleen cells derived from ebi3(-/-) mice produce significantly higher levels of il-17 as well as il-22 upon stimulation with ova. in vitro ... | 2008 | 18412165 |
| fate of foodborne pathogens on green onions and tomatoes by electrolysed water. | to investigate the efficacy of electrolysed water (ew) in killing escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes on the surfaces of spot-inoculated green onions and tomatoes. | 2008 | 18416702 |
| effects of dielectrophoresis on growth, viability and immuno-reactivity of listeria monocytogenes. | abstract: dielectrophoresis (dep) has been regarded as a useful tool for manipulating biological cells prior to the detection of cells. since dep uses high ac electrical fields, it is important to examine whether these electrical fields in any way damage cells or affect their characteristics in subsequent analytical procedures. in this study, we investigated the effects of dep manipulation on the characteristics of listeria monocytogenes cells, including the immuno-reactivity to several listeria ... | 2008 | 18416836 |
| horizontal gene transfer of virulence determinants in selected bacterial foodborne pathogens. | this review describes horizontal gene transfer from a historical point of view, with descriptions of the first instances of the different bacterial transfer mechanisms: conjugation, transduction and transformation, as well as examples of some of the early acknowledged transfer events. gene transfer from four selected foodborne pathogens: escherichia coli, listeria monocytogenes,staphylococcus aureus and salmonella are highlighted. | 2009 | 18420327 |
| the role of horizontal gene transfer in the evolution of selected foodborne bacterial pathogens. | bacteria use various ways to transfer genetic information. these methods include: conjugation, which requires cell to cell contact between cells, transduction, which is bacteriophage-facilitated transfer of genetic information, and transformation, which is the uptake of free dna from the environment. usually the genes to be transferred lie on mobile genetic elements, pieces of dna that encode proteins important to facilitate movement of dna within or between genomes. this review highlights the t ... | 2009 | 18420329 |
| hematopoietic response of rats exposed to the impact of an acute psychophysiological stressor on responsiveness to an in vivo challenge with listeria monocytogenes: modulation by chlorella vulgaris prophylactic treatment. | in this study, we investigated the hematopoietic response of rats pretreated with cv and exposed to the impact of acute escapable, inescapable or psychogenical stress on responsiveness to an in vivo challenge with listeria monocytogenes. no consistent changes were observed after exposure to escapable footshock. conversely, the impact of uncontrollable stress (inescapable and psychogenical) was manifested by an early onset and increased severity and duration of myelossuppression produced by the i ... | 2008 | 18420376 |
| microbial and sensory changes throughout the ripening of prato cheese made from milk with different levels of somatic cells. | the objective of this research was to evaluate the effects of 2 levels of raw milk somatic cell count (scc) on the composition of prato cheese and on the microbiological and sensory changes of prato cheese throughout ripening. two groups of dairy cows were selected to obtain low-scc (<200,000 cells/ml) and high-scc (>700,000 cells/ml) milks, which were used to manufacture 2 vats of cheese. the pasteurized milk was evaluated according to the ph, total solids, fat, total protein, lactose, standard ... | 2008 | 18420604 |
| magnetic resonance images in rhombencephalitis due to listeria monocytogenes mimicking wernicke encephalopathy. | | 2008 | 18421210 |
| chitin hydrolysis by listeria spp., including l. monocytogenes. | listeria spp., including the food-borne pathogen listeria monocytogenes, are ubiquitous microorganisms in the environment and thus are difficult to exclude from food processing plants. the factors that contribute to their multiplication and survival in nature are not well understood, but the ability to catabolize various carbohydrates is likely to be very important. one major source of carbon and nitrogen in nature is chitin, an insoluble linear beta-1,4-linked polymer of n-acetylglucosamine (gl ... | 2008 | 18424542 |
| prevention of allograft tolerance by bacterial infection with listeria monocytogenes. | exposure to certain viruses and parasites has been shown to prevent the induction of transplantation tolerance in mice via the generation of cross-reactive memory t cell responses or the induction of bystander activation. bacterial infections are common in the perioperative period of solid organ allograft recipients in the clinic, and correlations between bacterial infections and acute allograft rejection have been reported. however, whether bacterial infections at the time of transplantation ha ... | 2008 | 18424719 |
| genome classification by gene distribution: an overlapping subspace clustering approach. | genomes of lower organisms have been observed with a large amount of horizontal gene transfers, which cause difficulties in their evolutionary study. bacteriophage genomes are a typical example. one recent approach that addresses this problem is the unsupervised clustering of genomes based on gene order and genome position, which helps to reveal species relationships that may not be apparent from traditional phylogenetic methods. | 2008 | 18430250 |
| infected ruptured popliteal artery aneurysm by listeria monocytogenes. a case report and review of the literatures. | we describe a case of a voluminous infected aneurysm of the popliteal artery, with listeria monocytogenes (lm) associated with rupture, in a 72-year old man. after radical resection of the aneurysm a reconstruction was not necessary, because of the sufficient blood supply, due to the pre-existent good development of collateral circulation. the patient was discharged on the 12th postoperative day with primary healing of the wound and viable leg. adequate antibiotic treatment was continued for 4 w ... | 2008 | 18431345 |
| choosing and preparing antigen-presenting cells. | the first issue in many antigen-processing experiments is to choose and obtain appropriate antigen-presenting cells (apcs). macrophages have characteristics that dictate their use in certain antigen-processing experiments, so this unit describes preparation of activated macrophages, which involves eliciting peritoneal macrophages from mice by infection with listeria monocytogenes. this method is often preferred because the resulting macrophages are more consistently activated to a high degree, b ... | 2001 | 18432740 |
| animal model for infection with listeria monocytogenes. | this unit describes methods for infecting mice with l. monocytogenes. optimal media for growth and methods to maintain bacterial virulence by passage through mice are included. methods for determining the severity of splenic and hepatic infection are detailed, with strategies for distinguishing innate from specific immune responses following l. monocytogenes infection. this infection induces mhc class i-restricted cd8(+) cytolytic t lymphocytes that clear infection and provide long-term immunity ... | 2001 | 18432760 |
| liposome-encapsulated antigens induce a protective ctl response against listeria monocytogenes independent of cd4+ t cell help. | protection against intracellular pathogens is usually mediated by cytotoxic t lymphocytes (ctl). induction of a protective ctl response for vaccination purposes has proven difficult because of the limited access of protein antigens or attenuated pathogens to the mhc class i presentation pathway. we show here that ph-sensitive pe/chems liposomes can be used as a vehicle to efficiently deliver intact proteins for presentation by mhc class i. mice immunized with listerial proteins encapsulated in s ... | 2008 | 18433404 |
| [listeria monocytogenes abscess of the brain]. | listeriosis commonly involves the central nervous system. meningoencephalitis and rhomboencephalitis are the most frequent manifestations. brain abscesses are rare. | 2008 | 18439933 |
| response regulator degu of listeria monocytogenes controls temperature-responsive flagellar gene expression in its unphosphorylated state. | we demonstrate that in listeria monocytogenes, temperature-responsive transcriptional control of flagellar genes does not rely on the phosphorylation of the conserved phosphorylation site (d55) in the receiver domain of response regulator degu. furthermore, proper control of degu-regulated genes involved in ethanol tolerance and virulence is independent of receiver phosphorylation. | 2008 | 18441066 |
| tools for functional postgenomic analysis of listeria monocytogenes. | we describe the development of genetic tools for regulated gene expression, the introduction of chromosomal mutations, and improved plasmid transfer by electroporation in the food-borne pathogen listeria monocytogenes. pimk, a kanamycin-resistant, site-specific, integrative listeriophage vector was constructed and then modified for overexpression (pimk2) or for isopropyl-beta-d-thiogalactopyranoside (iptg)-regulated expression (pimk3 and pimk4). the dynamic range of promoters was assessed by det ... | 2008 | 18441118 |
| recombinant listeria monocytogenes expressing an immunodominant peptide fails to protect after intravaginal challenge with herpes simplex virus-2. | recombinant listeria monocytogenes expressing a type-common herpes simplex virus (hsv) gb-peptide was shown previously to protect against footpad inoculation with hsv-1. we tested this construct for protection against vaginal challenge with hsv-2. primed mice demonstrated strong recall responses, had modest reductions in hsv-2 dna in vaginal mucosa, but were not protected from disease. | 2008 | 18443737 |
| [investigation of listeria monocytogenes serotype o antibodies in maternal and cord sera and the evaluation of risk factors for listeriosis in pregnant women]. | listeria monocytogenes may lead to intrauterine infections which can be treated if diagnosed promptly. however, there is not a rapid routine screening test with high specificity and sensitivity for the diagnosis of listeriosis during pregnancy. we investigated the presence of different l. monocytogenes o antibodies for diagnosis of listeriosis in 275 paired maternal-cord sera using the agglutination test, and aimed to evaluate the correlation between poor pregnancy outcomes, level of l. monocyto ... | 2008 | 18444561 |
| human listeria monocytogenes infections in europe--an opportunity for improved european surveillance. | the 2006 community summary report from the european food safety authority (efsa) and the european centre for disease prevention and control (ecdc) was published recently with the latest trends and figures on the occurrence of zoonotic infections and agents, antimicrobial resistance and foodborne outbreaks in the then 25 european union (eu) member states and five non-eu countries. this article seeks to expand further upon reports of human listeriosis (listeria monocytogenes infections) and change ... | 2008 | 18445429 |
| survival of selected pathogens in diluted sludge of a thermophilic waste treatment plant and in nacl-solution under aerobic and anaerobic conditions. | decimal reduction times (drt or d-value) of campylobacter jejuni, salmonella enterica (formerly salmonella choleraesuis) serovar senftenberg, escherichia coli, and listeria monocytogenes were determined in two different matrices, diluted fermenter sludge (dfs) and 0.95% nacl-solution (nacl) at 50 degrees c, both under aerobic and anaerobic conditions. depending on aeration, matrix composition, and the respective organism, the d-values varied between 10min and more than 15h. generally the viabili ... | 2009 | 18445518 |
| method to detect only live bacteria during pcr amplification. | ethidium monoazide (ema) is a dna cross-linking agent and eukaryotic topoisomerase ii poison. we previously reported that the treatment of ema with visible light irradiation (ema + light) directly cleaved chromosomal dna of escherichia coli (t. soejima, k. iida, t. qin, h. taniai, m. seki, a. takade, and s. yoshida, microbiol. immunol. 51:763-775, 2007). herein, we report that ema + light randomly cleaved chromosomal dna of heat-treated, but not live, listeria monocytogenes cells within 10 min o ... | 2008 | 18448692 |
| in vivo hierarchy of individual t-cell epitope-specific helper t-cell subset against an intracellular bacterium. | cellular immunity is indispensable for efficient protection against intracellular bacterial infection. cd4+ and cd8+ t cells specific for a variety of antigenic peptides derived from particular bacteria are induced after the infection. t cells recognizing different antigenic peptides have been speculated to have different functions in terms of the protective immunity. we here induced individual cd4+ t cells specific for each antigenic peptide derived from listeria monocytogenes independently wit ... | 2008 | 18450341 |
| naive and innate memory phenotype cd4+ t cells have different requirements for active itk for their development. | the tec family kinase itk regulates the development of conventional and innate cd8(+) t cells. however, little is known about the role of itk in the development of cd4(+) t cell lineages, although the role of itk in the t cell activation and function is well defined. we show in this study that itk null mice have increased percentage of cd62l(low)cd44(high) memory phenotype cd4(+) t cells compared with wild-type mice. these cells arise directly in the thymus, express high levels of transcripts fo ... | 2008 | 18453573 |
| additive roles for mcp-1 and mcp-3 in ccr2-mediated recruitment of inflammatory monocytes during listeria monocytogenes infection. | chemokine receptor-mediated recruitment of inflammatory cells is essential for innate immune defense against microbial infection. recruitment of ly6c(high) inflammatory monocytes from bone marrow to sites of microbial infection is dependent on ccr2, a chemokine receptor that responds to mcp-1 and mcp-3. although ccr2(-/-) mice are markedly more susceptible to listeria monocytogenes infection than are wild-type mice, mcp-1(-/-) mice have an intermediate phenotype, suggesting that other ccr2 ligan ... | 2008 | 18453605 |
| characterization of virulence properties of listeria monocytogenes serotype 4b strains of different origins. | in this study, the virulence heterogeneity of listeria monocytogenes serotype 4b strains of different origins was analysed on different levels. on one hand, the survival of l. monocytogenes strains in synthetic gastric fluid was studied. on the other hand, the pathogenic potential of strains with different inlb expression levels was analysed in an a/j mouse model for gastrointestinal listeriosis. differences in survival capacity in gastric fluid and in in vivo virulence potential were observed b ... | 2008 | 18454745 |
| listeriosis in pregnancy. | | 2008 | 18455101 |
| macrophages express multiple ligands for gammadelta tcrs. | as only a handful of ligands have been identified, the general nature of the ligands recognized by gammadelta t cells remains unresolved. in this study, soluble multimerized gammadelta t cell receptors (smtcrs) representing the tcrs of two gammadelta t cell subsets common in the mouse were used to detect and track their own ligands. ligands for both subsets were found on resident peritoneal macrophages taken from untreated mice, and the expression of both was further induced by listeria monocyto ... | 2008 | 18455236 |
| acupuncture-associated listeria monocytogenes arthritis in a patient with rheumatoid arthritis. | septic arthritis is a rare complication of acupuncture. we present a patient with rheumatoid arthritis who developed septic arthritis of the right knee after consecutive weekly sessions of acupuncture therapy for 3 weeks. the infection was localized by musculoskeletal sonography and magnetic resonance imaging, with culture of the synovial fluid aspirated from the joint yielding listeria monocytogenes. the patient responded well to antibiotic treatment and regained joint mobility. a high index of ... | 2008 | 18455948 |
| combined effect of freeze chilling and map on quality parameters of raw chicken fillets. | the effect of short-term frozen storage prior to thawing on the quality of freeze-chilled chicken fillets was investigated, as was the effect of modified atmosphere packaging (map). four process treatments were used: (1) fresh chicken chilled at 4 degrees c without previous freezing, (2) freeze-chilled for 7 days and thawed at 4 degrees c, (3) chilled at 4 degrees c packaged under map (70% n(2)-30%co(2)), and (4) packaged under map, freeze-chilled for 7 days and thawed at 4 degrees c. microbiolo ... | 2008 | 18456112 |
| identification of a non-pathogenic surrogate organism for chlorine dioxide (clo2) gas treatment. | the identification of non-pathogenic surrogate microorganisms is beneficial for determining and validating the efficacy of antimicrobial treatments in food manufacturing environments. a surrogate organism was identified to aid in the decontamination process of fresh produce when treated with chlorine dioxide (clo(2)) gas. thirty-two known strains of pathogenic and non-pathogenic microorganisms and seven unknown microbial isolates from mushroom, tomatoes, and strawberries were evaluated. the prim ... | 2008 | 18456115 |
| characteristics of an environmental strain, enterococcus faecalis cect7121, and its effects as additive on craft dry-fermented sausages. | lactic acid bacteria are the most adequate microorganisms for natural preservation of food. in the present work, the strain of enterococcus faecalis cect7121 was employed in the manufacture of craft dry-fermented sausages and its performance as a biopreservative was analysed. this strain is devoid of the genes for haemolysin and gelatinase and does not produce biogenic amines. it is sensitive to almost all the antibiotics tested and opsonophagocytic assays showed that it is devoid of a capsule. ... | 2008 | 18456116 |
| microbial changes and growth of listeria monocytogenes during chilled storage of brined shrimp (pandalus borealis). | thirteen storage trials and ten challenge tests were carried out to examine microbial changes, spoilage and the potential growth of listeria monocytogenes in brined shrimp (pandalus borealis). shrimp in brine as well as brined and drained shrimp in modified atmosphere packaging (map) were produced and studied. different recipes were used to study the effect of preserving parameters (organic acids, ph and nacl) on growth of microorganisms and shelf life at 7-8 degrees c or 12 degrees c. particula ... | 2008 | 18456355 |
| evaluation of an enumeration method for listeria monocytogenes at low contamination levels in cold-smoked salmon. | for the enumeration of listeria monocytogenes in cold-smoked salmon, a sensitive enumeration method, based on membrane filtration followed by transfer of the filter on a selective medium has been recently developed (gnanou besse et al., 2004, a contribution to the improvement of l. monocytogenes enumeration in cold-smoked salmon. international journal of food microbiology, 91, 119-127). the aim of the study was to assess the performance of this enumeration method through an inter-laboratory stud ... | 2008 | 18456358 |
| the two-component system cesrk controls the transcriptional induction of cell envelope-related genes in listeria monocytogenes in response to cell wall-acting antibiotics. | the two-component system cesrk of listeria monocytogenes responds to cell wall-acting antibiotics. we show here that cesrk controls the transcription of several cell envelope-related genes. the cesrk-dependent induction of these genes may be viewed as an attempt by l. monocytogenes to protect itself against the damaging effects of cell wall-acting antibiotics. | 2008 | 18456805 |
| development of a multilocus variable-number of tandem repeat typing method for listeria monocytogenes serotype 4b strains. | listeria monocytogenes serotype 4b strains have been identified as the causative agent in many human listeriosis epidemics as well as in a considerable number of sporadic cases. due to the genetic homogeneity of serotype 4b isolates, development of rapid subtyping methods with high discriminatory power for serotype 4b isolates is required to allow for improved outbreak detection and source tracking. in this study, multilocus variable-number tandem repeat analysis (mlva) was developed and used to ... | 2008 | 18457891 |
| identification of listeria innocua surrogates for listeria monocytogenes in hamburger patties. | listeria innocua m1 has been used by many researchers as a nonpathogenic thermal processing surrogate for listeria monocytogenes. however, l. innocua m1 has been criticized because its thermal survivability characteristics are not as closely parallel to l. monocytogenes as some would like in a variety of foods and processing conditions. the present study was conducted to compare multiple l. innocua and l. monocytogenes strains to validate l. innocua m1 as the ideal surrogate under high-temperatu ... | 2008 | 18460133 |
| characterization of bacteriocin-coated antimicrobial polyethylene films by atomic force microscopy. | antimicrobial agents can be used to give antimicrobial properties to polymeric materials used to envelope foods for packaging purposes. in this study, we exploited an atomic force microscopy (afm) analysis for the characterization of plastic films activated with antimicrobial agents. the aim was to acquire information on the distribution of the antimicrobials on plastic materials with the ultimate scope of understanding the mechanisms of interaction between antimicrobials and materials to be use ... | 2008 | 18460145 |
| survival of bacterial pathogens during the thermophilic anaerobic digestion of biowaste: laboratory experiments and in situ validation. | anaerobic digestion is continually gaining importance for the processing of the organic fraction of municipal solid wastes. although methods for studying the survival of pathogen exist, these methods often need adaptations, are expensive, time consuming or generally not well suited for the harsh conditions within an anaerobic digestion system. in the present study we investigated the applicability of commercially available, mechanically stable and inexpensive pathogen carriers to validate in sit ... | 2008 | 18460419 |
| cd14 works with toll-like receptor 2 to contribute to recognition and control of listeria monocytogenes infection. | toll-like receptor 2 (tlr2) signaling has been shown to contribute to resistance to listeria monocytogenes infection, as tlr2-deficient mice have a heightened susceptibility to infection with this organism. because cd14 may associate with tlr2, we investigated the role of cd14 in listeria responses. in both cd14-deficient and tlr2-deficient macrophages, nuclear factor kappab translocation; cd40 and cd86; and the production of interleukin (il)-12, il-6, tumor necrosis factor, and nitric oxide are ... | 2008 | 18462086 |
| listeria monocytogenes: epidemiology, human disease, and mechanisms of brain invasion. | listeria monocytogenes is a facultative intracellular bacterium that has predilection for causing central nervous systemic infections in humans and domesticated animals. this pathogen can be found worldwide in the food supply and most l. monocytogenes infections are acquired through ingestion of contaminated food. the main clinical syndromes caused by l. monocytogenes include febrile gastroenteritis, perinatal infection, and systemic infections marked by central nervous system infections with or ... | 2008 | 18462388 |
| antimicrobial activity of some new thioureides derived from 2-(4-chlorophenoxymethyl)benzoic acid. | we report here the characterisation of eight newly synthesized thioureides of 2-(4-chlorophenoxymethyl)-benzoic acid and the evaluation of the in vitro antimicrobial activity of the new compounds against gram-positive [listeria monocytogenes,staphylococcus aureus, bacillus subtilis], gram-negative [psedomonas aeruginosa,escherichia coli, salmonella enteritidis], as well as candida spp., using both reference and clinical multidrug resistant strains to establish the minimal inhibitory concentratio ... | 2008 | 18463566 |
| a case of listeria meningoencephalitis complicated by hydrocephalus in an immunocompetent infant. | we report a very unusual case of meningoencephalitis due to listeria monocytogenes in a 7-month-old immunocompetent boy. cerebrospinal fluid (csf) culture was initially negative, but was positive on the seventh day. the disease was complicated by seizures and hydrocephalus managed with temporary ventriculostomy. the infant was discharged without obvious neurological sequelae after 30 days and developed without neurological or developmental sequelae at two years of age. listeria is difficult to i ... | 2009 | 18463893 |
| circumvention of regulatory cd4(+) t cell activity during cross-priming strongly enhances t cell-mediated immunity. | immunization with purified antigens is a safe and practical vaccination strategy but is generally unable to induce sustained cd8(+) t cell-mediated protection against intracellular pathogens. most efforts to improve the cd8(+) t cell immunogenicity of these vaccines have focused on co-administration of adjuvant to support cross-presentation and dendritic cell maturation. in addition, it has been shown that cd4(+) t cell help during the priming phase contributes to the generation of protective cd ... | 2008 | 18465771 |
| control of listeria monocytogenes on vacuum-packaged frankfurters sprayed with lactic acid alone or in combination with sodium lauryl sulfate. | u.s. regulations require that processors employ lethal or inhibitory antimicrobial alternatives in production of ready-to-eat meat and poultry products that support growth of listeria monocytogenes and may be exposed to the processing environment after a lethality treatment. in this study, lactic acid (la; 5%, vol/vol) and sodium lauryl sulfate (sls; 0.5%, wt/vol) were evaluated individually or as a mixture (lasls) for control of l. monocytogenes on frankfurters. frankfurters were inoculated wit ... | 2008 | 18468026 |
| monitoring hygiene on- and at-line is critical for controlling listeria monocytogenes during produce processing. | the prevalence of listeria monocytogenes in different types of produce and on processing plant environments was investigated over a 4-year period in a large produce processing plant in poland. prevalence of l. monocytogenes was 46% in frozen vegetables and 41.3% in swab samples taken from the plant environment. survival studies using artificial inocula demonstrated that the number of listeria in frozen produce stored for 100 days did not significantly decrease in relation to the initial contamin ... | 2008 | 18468027 |
| a radial basis function neural network approach to determine the survival of listeria monocytogenes in katiki, a traditional greek soft cheese. | a radial basis function neural network was developed to determine the kinetic behavior of listeria monocytogenes in katiki, a traditional white acid-curd soft spreadable cheese. the applicability of the neural network approach was compared with the reparameterized gompertz, the modified weibull, and the geeraerd primary models. model performance was assessed with the root mean square error of the residuals of the model (rmse), the regression coefficient (r2), and the f test. commercially prepare ... | 2008 | 18468029 |
| modification of the submerged coil to prevent microbial carryover error in thermal death studies. | a submerged coil unit generates death rate data for foodborne pathogens through precise computer-controlled sequential sampling rather than the usual manually timed, labor-intensive single sampling associated with other approaches. our work with yersinia pseudotuberculosis and listeria monocytogenes scott a using the submerged coil unit indicated non-log-linear death rates with large degrees of tailing. varying degrees of cell adhesion to the surface of the exit port resulted in carryover that w ... | 2008 | 18468032 |
| enhanced rapidity for qualitative detection of listeria monocytogenes using an enzyme-linked immunosorbent assay and immunochromatography strip test combined with immunomagnetic bead separation. | an enzyme-linked immunosorbent assay (elisa), immunochromatography (icg) strip test, and immunomagnetic bead separation (imbs) system based on a monoclonal antibody were individually developed for the detection and isolation of listeria monocytogenes in meat samples. the three methods showed a strong reaction with listeria species and a weak reaction with staphylococcus aureus. to increase the rapidity of l. monocytogenes detection, combinations of the elisa and icg strip test with the imbs syst ... | 2008 | 18468033 |
| prevalence of listeria monocytogenes and salmonella in ready-to-eat food in catalonia, spain. | listeria monocytogenes and salmonella are pathogenic bacteria that can contaminate food products during or after processing. ready-to-eat (rte) food does not undergo any treatment to ensure its safety before consumption, and therefore risk of foodborne disease must be considered if these pathogens are present in the food. to evaluate the prevalence of these pathogens in rte food, 140 rte fish product samples, 501 rte meat product samples, 462 rte dairy samples, and 123 rte dishes and desserts, p ... | 2008 | 18468047 |
| microbiological quality of sushi from sushi bars and retailers. | sushi is a traditional japanese food, mostly consisting of rice and raw fish. fish is considered a healthy food, but as with other animal products, consumption of raw muscle incurs potential health risks such as ingestion of pathogenic bacteria or parasites. in this study, 250 sushi samples were analyzed for their microbiological status and the prevalence of pathogenic bacteria. a comparison was made between frozen sushi from supermarkets and fresh sushi from sushi bars. aerobic mesophilic bacte ... | 2008 | 18468048 |
| endocarditis due to listeria: description of two cases and review of the literature. | endocarditis due to listeria monocytogenes is a very uncommon and very serious disease that may lead to valve dysfunction and cardiac arrest. we report two cases of endocarditis caused by l. monocytogenes and review the papers previously published on the subject. | 2008 | 18471682 |
| validation of nmkl method no. 136--listeria monocytogenes, detection and enumeration in foods and feed. | a collaborative study was organised to define the performance characteristics of the revised nmkl method no.136 "listeria monocytogenes. detection and enumeration in foods". chromogenic l. monocytogenes specific plating medium, agar listeria according to ottaviani and agosti (aloa) was introduced in the revised method in order to improve the sensitivity and specificity of the method, and to shorten the analysis time. efficacy of aloa one day from aes (ready-to-use agar in bottles), listeria chro ... | 2008 | 18472176 |
| selected prfa* mutations in recombinant attenuated listeria monocytogenes strains augment expression of foreign immunogens and enhance vaccine-elicited humoral and cellular immune responses. | while recombinant listeria monocytogenes strains can be explored as vaccine candidates, it is important to develop attenuated but highly immunogenic l. monocytogenes vaccine vectors. here, prfa* mutations selected on the basis of upregulated expression of l. monocytogenes prfa-dependent genes and proteins were assessed to determine their abilities to augment expression of foreign immunogens in recombinant l. monocytogenes vectors and therefore enhance vaccine-elicited immune responses (a prfa* m ... | 2008 | 18474644 |
| first trimester listeria monocytogenes septicemia. | background: little is known about fetal outcome after listeria monocytogenes septicemia in the first trimester of pregnancy.case: a primigravida with l. monocytogenes septicemia at 9 weeks gestation was treated with amoxicillin. at 40 weeks gestation a healthy female infant was born.conclusion: this case shows that fetal outcome can be normal after appropriate treatment of maternal l. monocytogenes septicemia during embryogenesis. | 1997 | 18476140 |
| [construction and characterization of a mutant strain of listeria monocytogenes with a deletion of acta and plcb]. | the virulence of listeria monocytogenes is directly related to its ability to spread from cell to cell without leaving the intracellular milieu. among the bacterial factors involved in cell-to-cell spread, actin-polymerizing protein acta is required for bacterial spread to adjacent cells, while the broad-range phospholipase c (pc-plc) contributes to bacterial escape from secondary vacuoles. | 2008 | 18479054 |
| [bacteremia, brain abscess, and meningitis caused by listeria monocytogenes]. | | 2008 | 18479654 |
| protease treatment affects both invasion ability and biofilm formation in listeria monocytogenes. | listeria monocytogenes is a notably invasive bacterium associated with life-threatening food-borne disease in humans. several surface proteins have been shown to be essential in the adhesion of l. monocytogenes, and in the subsequent invasion of phagocytes. because the control of the invasion of host cells by listeria could potentially hinder its spread in the infected host, we have examined the effects of a protease treatment on the ability of l. monocytogenes to form biofilms and to invade tis ... | 2008 | 18479885 |
| [primary listeria monocytogenes infection in a cirrhotic woman: report of one case]. | l. monocytogenes infections are infrequent. sepsis in pregnant women and newborns and central nervous system infections in the elderly are the most common clinical manifestations. we report a 61 years old woman with diabetes mellitus and a child b hepatic cirrhosis, admitted for persistent fever. blood cultures were positive for listeria monocytogenes. cerebrospinal fluid was normal and sterile. she was treated with ampicillin and amikacin with a good response. control blood cultures were negati ... | 2008 | 18483678 |
| the generation of nisin variants with enhanced activity against specific gram-positive pathogens. | nisin is the prototype of the lantibiotic group of antimicrobial peptides. it exhibits broad spectrum inhibition of gram-positive bacteria including important food pathogens and clinically relevant antibiotic-resistant bacteria. significantly, the gene-encoded nature of nisin means that it can be subjected to gene-based bioengineering to generate novel derivatives. here, we take advantage of this to generate the largest bank of randomly mutated nisin derivatives reported to date, with the ultima ... | 2008 | 18485077 |
| acid sphingomyelinase is required for efficient phago-lysosomal fusion. | the acid sphingomyelinase (asmase) localizes to the lumen of endosomes, phagosomes and lysosomes as well as to the outer leaflet of the plasma membrane and hydrolyses sphingomyelin to ceramide and phosphorylcholine. using the facultative intracellular bacterium listeria monocytogenes, we show that maturation of phagosomes into phagolysosomes is severely impaired in macrophages genetically deficient for asmase. unlike in wild-type macrophages, phagosomes containing l. monocytogenes in asmase(-/-) ... | 2008 | 18485117 |
| synergistic effect between different milk-derived peptides and proteins. | antimicrobial peptides derived from food proteins constitute a new field in the combined use of antimicrobial agents in food. the best examples of milk-derived peptides are those constituted by bovine lactoferricin [lactoferrin f(17-41)] (lfcin-b) and bovine alpha(s2)-casein f(183-207). the aim of this work was to study if the antimicrobial activity of a natural compound employed in food preservation, nisin, could be enhanced by combination with the aforementioned milk-derived peptides. furtherm ... | 2008 | 18487640 |
| listeria monocytogenes and salmonella spp. in raw milk produced in brazil: occurrence and interference of indigenous microbiota in their isolation and development. | this study aimed to verify the occurrence of listeria monocytogenes and salmonella spp. in raw milk produced in brazil. on account of the poor microbiological quality of this product, possible interference from the indigenous microbiota in these pathogens was also evaluated. two-hundred and ten raw milk samples were collected in four important milk-producing areas in brazil, tested for l. monocytogenes and salmonella spp. presence, and for enumeration of indicator microorganisms: mesophilic aero ... | 2008 | 18489543 |
| pdl-1 blockade impedes t cell expansion and protective immunity primed by attenuated listeria monocytogenes. | infection with attenuated listeria monocytogenes (lm) is a robust in vivo model for examining how ag-specific t cells are primed, and subsequent challenge with virulent lm allows for the protective effects of t cell priming to be quantified. herein, we investigated the role of programmed death ligand 1 (pdl-1) in t cell priming and immunity conferred after primary infection with lm deltaacta followed by virulent lm challenge. in striking contrast to the inhibitory role of pdl-1 on t cell immunit ... | 2008 | 18490756 |
| multiple nod-like receptors activate caspase 1 during listeria monocytogenes infection. | listeria monocytogenes escapes from the phagosome of macrophages and replicates within the cytosolic compartment. the macrophage responds to l. monocytogenes through detection pathways located on the cell surface (tlrs) and within the cytosol (nod-like receptors) to promote inflammatory processes aimed at clearing the pathogen. cytosolic l. monocytogenes activates caspase 1, resulting in post-translational processing of the cytokines il-1beta and il-18 as well as caspase 1-dependent cell death ( ... | 2008 | 18490757 |
| listeriosis: a model for the fine balance between immunity and morbidity. | listeriosis is a severe food-borne disease caused by listeria monocytogenes. it mostly affects immune-compromised individuals, pregnant women, and the elderly, and it is associated with huge economic losses, especially to the food industry. in the last decade, a sharp increase in listeriosis incidence was observed in several european countries. no suitable explanation was found for this increase, which occurred only in old patients and not in pregnant women. | 2008 | 18496469 |
| lineage specific recombination and positive selection in coding and intragenic regions contributed to evolution of the main listeria monocytogenes virulence gene cluster. | the major virulence cluster of listeria monocytogenes harbors six virulence genes that encode proteins critical for the intracellular life cycle of this human and animal pathogen. in this study, we determined the sequence (8709nt) of the virulence gene cluster (including the six main virulence genes) in 40 l. monocytogenes isolates from different source populations (human clinical cases, animal clinical cases, foods, and natural environments). an alignment of the full length cluster as well as i ... | 2008 | 18499533 |
| glycerol metabolism and prfa activity in listeria monocytogenes. | listeria monocytogenes is able to efficiently utilize glycerol as a carbon source. in a defined minimal medium, the growth rate (during balanced growth) in the presence of glycerol is similar to that in the presence of glucose or cellobiose. comparative transcriptome analyses of l. monocytogenes showed high-level transcriptional upregulation of the genes known to be involved in glycerol uptake and metabolism (glpfk and glpd) in the presence of glycerol (compared to that in the presence of glucos ... | 2008 | 18502850 |
| coordinated regulation of cold-induced changes in fatty acids with cardiolipin and phosphatidylglycerol composition among phospholipid species for the food pathogen listeria monocytogenes. | we present here the structural identification of four phospholipid (phl) classes in listeria monocytogenes, the fatty acid (fa) composition for each individual phl species, and a description of cold-induced fa changes. cardiolipin (48.5%) and phosphatidylglycerol (18.1%) are dominated by anteiso-fa, and the previously recognized branched fa chain shortening by cold was observed singularly in these phls. phosploaminolipid (19.9%) and phosphatidylinositol, (9.1%) are significantly different, conta ... | 2008 | 18502923 |