| combined effect of aqueous chlorine dioxide and modified atmosphere packaging on inhibiting salmonella typhimurium and listeria monocytogenes in mungbean sprouts. | this study was conducted to investigate the effect of chlorine dioxide (clo2) combined with modified atmosphere packaging (map) on inhibiting total mesophilic microorganisms, salmonella typhimurium, and listeria monocytogenes in mungbean sprouts during refrigerated storage. mungbean sprouts were packaged using 4 different methods (air, vacuum, co2 gas, and n2 gas) following treatment with water or 100 ppm clo2 for 5 min and stored at 5 +/- 2 degrees c. the population of total mesophilic microorg ... | 2007 | 18034740 |
| a role for translational control in listerial osmoregulation and strain variation? | | 2007 | 18041949 |
| phenotypic and molecular characterization of listeria monocytogenes strains isolated from a marine environment in morocco. | microbiological analysis of 1025 marine samples, including 345 from seawater, 337 from shellfish, and 343 from sediments collected between january 2000 and december 2002 from 18 shellfish sites on the atlantic coast of mid-west of morocco (agadir region), yielded 143 strains of listeria (listeria monocytogenes: 38; l. innocua: 109; l. ivanovii: 1). the overall incidence of listeria sp. in the coastal environment was 5.3%. thirteen l. monocytogenes strains were isolated from seawater, 7 from sedi ... | 2007 | 18041951 |
| antibacterial activity of guava (psidium guajava l.) and neem (azadirachta indica a. juss.) extracts against foodborne pathogens and spoilage bacteria. | the antibacterial activity of guava (psidium guajava) and neem (azadirachta indica) extracts against 21 strains of foodborne pathogens were determined--listeria monocytogenes (five strains), staphylococcus aureus (four strains), escherichia coli o157:h7 (six strains), salmonella enteritidis (four strains), vibrio parahaemolyticus, and bacillus cereus, and five food spoilage bacteria: pseudomonas aeroginosa, p. putida, alcaligenes faecalis, and aeromonas hydrophila (two strains). guava and neem e ... | 2007 | 18041957 |
| how university researchers can contribute to farm-to-table risk assessments: listeria monocytogenes as an example. | as a part of the sanitary and phytosanitary (sps) agreement, the world trade organization requires that member countries establish sps measures on the basis of an appropriate risk assessment. in addition, many governments use risk assessment in their management of food safety. consequently, a number of risk assessments for different foodborne pathogens have been conducted. risk assessments have also been successfully used as a research tool. while, historically, risk assessments are typically in ... | 2007 | 18041962 |
| inhibition of the rage products increases survival in experimental models of severe sepsis and systemic infection. | the receptor for advanced glycation end products (rage), a multi-ligand member of the immunoglobulin superfamily, contributes to acute and chronic disease processes, including sepsis. | 2007 | 18042296 |
| effect of biofilm dryness on the transfer of listeria monocytogenes biofilms grown on stainless steel to bologna and hard salami. | listeria monocytogenes continues to be a major cause of class i food recalls in the united states. very little is known about its transfer and cross-contamination in processing scenarios. the objective of this study was to evaluate the effect of hydration level on l. monocytogenes biofilms grown on stainless steel and its effect on the biofilm transfer to foods. biofilms were grown on stainless steel in diluted tryptic soy broth 1:20 for 48 h at 32 degrees c. after this, biofilms were equilibrat ... | 2007 | 18044424 |
| modeling and predicting the growth of lactic acid bacteria in lightly preserved seafood and their inhibiting effect on listeria monocytogenes. | a cardinal parameter model was developed to predict the effect of diacetate, lactate, co2, smoke components (phenol), ph, nacl, temperature, and the interactions between all parameters on the growth of lactic acid bacteria (lab) in lightly preserved seafood. a product-oriented approach based on careful chemical characterization and growth of bacteria in ready-to-eat seafoods was used to develop this new lab growth model. initially, cardinal parameter values for the inhibiting effect of diacetate ... | 2007 | 18044425 |
| inhibition of listeria monocytogenes in cooked ham through active packaging with natural antimicrobials and high-pressure processing. | enterocins a and b and sakacin k at 200 and 2,000 activity units (au)/cm2, nisin at 200 au/cm2, 1.8% potassium lactate, and a combination of 200 au/cm2 of nisin and 1.8% lactate were incorporated into interleavers, and their effectiveness against listeria monocytogenes spiked in sliced, cooked ham was evaluated. antimicrobial-packaged cooked ham was then subjected to high-pressure processing (hpp) at 400 mpa. in nonpressurized samples, nisin plus lactate-containing interleavers were the most eff ... | 2007 | 18044426 |
| effect of combining nisin and/or lysozyme with in-package pasteurization on thermal inactivation of listeria monocytogenes in ready-to-eat turkey bologna. | achieving a targeted lethality with minimum exposure to heat and preservation of product quality during pasteurization is a challenge. the objective of this study was to evaluate the effect of nisin and/or lysozyme in combination with in-package pasteurization of a ready-to-eat low-fat turkey bologna on the inactivation of listeria monocytogenes. sterile bologna samples were initially treated with solutions of nisin (2 mg/ml = 5,000 au/ml = 31.25 au/cm2), lysozyme (10 mg/ml = 80 au/ml = 0.5 au/c ... | 2007 | 18044427 |
| listeria prevalence and listeria monocytogenes serovar diversity at cull cow and bull processing plants in the united states. | listeria monocytogenes, the causative agent of epidemic and sporadic listeriosis, is routinely isolated from many sources, including cattle, yet information on the prevalence of listeria in beef processing plants in the united states is minimal. from july 2005 through april 2006, four commercial cow and bull processing plants were sampled in the united states to determine the prevalence of listeria and the serovar diversity of l. monocytogenes. samples were collected during the summer, fall, win ... | 2007 | 18044437 |
| resistance of listeria monocytogenes f2365 cells to synthetic gastric fluid is greater following growth on ready-to-eat deli turkey meat than in brain heart infusion broth. | ready-to-eat (rte) deli meats have been categorized as high-risk foods for contraction of foodborne listeriosis. several recent listeriosis outbreaks have been associated with the consumption of rte deli turkey meat. in this study, we examined whether the growth of listeria monocytogenes f2365 on commercially prepared rte deli turkey meat causes listerial cells to become more resistant to inactivation by synthetic gastric fluid (sgf). listerial cells grown on turkey meat to late logarithmic-earl ... | 2007 | 18044439 |
| prevalence, types, and geographical distribution of listeria monocytogenes from a survey of retail queso fresco and associated cheese processing plants and dairy farms in sonora, mexico. | in the first part of this study, samples were collected from farms, cheese processing plants (cpps), and retail markets located in various geographical areas of sonora, mexico, over a 12-month period during the summer of 2004 and winter of 2005. four (all queso fresco [qf] from retail markets) of 349 total samples tested positive for listeria monocytogenes (lm). of these four positive samples, three were collected in the northern region and one in the southern region of sonora. additionally, two ... | 2007 | 18044440 |
| survival and growth of listeria monocytogenes in broth as a function of temperature, ph, and potassium lactate and sodium diacetate concentrations. | the objective of this study was to determine the antimicrobial effect of a combination of potassium lactate and sodium diacetate (0, 1.8, 3, and 4.5%; purasal p opti. form 4, 60% solution) on the survival and growth of listeria monocytogenes scott a in ph-adjusted broth (5.5, 6.0, 6.5, and 7.0) stored at 4, 10, 17, 24, 30, and 37 degrees c. appropriate dilutions of broth were enumerated by spiral plating on tryptose agar and counted with an automated colony counter. growth data were iteratively ... | 2007 | 18044445 |
| effects of essential oils of oregano and nutmeg on growth and survival of yersinia enterocolitica and listeria monocytogenes in barbecued chicken. | the in vitro effects of plant essential oils (eos) against pathogenic bacteria are well known, yet few studies have addressed the effects of these compounds against pathogens associated with ready-to-cook foods. experiments were conducted to determine the effectiveness of oregano and nutmeg eos on the growth and survival of yersinia enterocolitica and listeria monocytogenes in broth culture and in iranian barbecued chicken. ready-to-cook iranian barbecued chicken was prepared according to the co ... | 2007 | 18044446 |
| antimicrobial efficacy of eugenol microemulsions in milk against listeria monocytogenes and escherichia coli o157:h7. | the antimicrobial activity of eugenol microemulsions (eugenol encapsulated in surfactant micelles) in ultrahigh-temperature pasteurized milk containing different percentages of milk fat (0, 2, and 4%) was investigated. antimicrobial microemulsions were prepared from a 5% (wt) aqueous surfactant solution (surfynol 485w) with 0.5% (wt) eugenol. two strains each of listeria monocytogenes and escherichia coli o157:h7 previously shown to be the least and most resistant to the microemulsion in microbi ... | 2007 | 18044447 |
| determination of 5-log reduction times for food pathogens in acidified cucumbers during storage at 10 and 25 degrees c. | outbreaks of acid-resistant foodborne pathogens in acid foods with ph values below 4.0, including apple cider and orange juice, have raised concerns about the safety of acidified vegetable products. for acidified vegetable products with ph values between 3.3 and 4.6, previous research has demonstrated that thermal treatments are needed to achieve a 5-log reduction in the numbers of escherichia coli o157:h7, listeria monocytogenes, or salmonella enterica. for some acidified vegetable products wit ... | 2007 | 18044448 |
| impact of dilution ratios on listeria monocytogenes growth during university of vermont medium enrichment of deli meats. | in the u.s. department of agriculture (usda) method for listeria detection, a 25-g composite food sample is enriched in 225 ml of university of vermont medium (uvm), giving a detection limit of 0.04 cfu/g. however, in a recent large-scale four-state deli meat survey for l. monocytogenes, 125-g samples enriched in 1,125 ml of uvm were requested to increase the detection limit to 0.008 cfu/g. to circumvent problems associated with large volumes of uvm, the impact on l. monocytogenes growth of lowe ... | 2007 | 18044452 |
| the glutamate decarboxylase acid resistance mechanism affects survival of listeria monocytogenes lo28 in modified atmosphere-packaged foods. | the contribution of the glutamate decarboxylase (gad) acid resistance system to survival and growth of listeria monocytogenes lo28 in modified atmosphere-packaged foods was examined. | 2007 | 18045416 |
| virulence characterization and genotypic analyses of listeria monocytogenes isolates from food and processing environments in eastern china. | in this study, twenty l. monocytogenes food-related isolates collected from eastern china zhejiang province were compared by in vivo ld50 assays as well as in vitro cytopathic plaque forming assay. nineteen l. monocytogenes isolates (19/20) were as virulent as reference strain 10403s, while the isolate m4 had low pathogenicity. the unique isolate m4 fell into lineage iii based on the partial nucleotide variations of acta, while the other isolates belonged to the more common lineages i and ii. l. ... | 2008 | 18045718 |
| requirement of secondary lymphoid tissues for the induction of primary and secondary t cell responses against listeria monocytogenes. | activation of naive t cells is tightly controlled and depends on cognate interactions with professional antigen-presenting cells. we analyzed dependency on secondary lymphoid tissues for the activation of naive and memory cd4(+) and cd8(+) t cells following primary and secondary listeria monocytogenes infection, respectively. in splenectomized lymphotoxin-beta receptor-deficient mice, lacking all secondary lymphoid tissues, oral infection with l. monocytogenes failed to induce bacteria-specific ... | 2008 | 18050270 |
| physical properties of nisin-incorporated gelatin and corn zein films and antimicrobial activity against listeria monocytogenes. | edible films of gelatin and corn zein were prepared by incorporating nisin to the film-forming solutions. corn zein film with nisin of 12,000 iu/ml had an increase of 11.6 mpa in tensile strength compared with the control, whereas gelatin film had a slight increase with the increase of nisin concentration added. water vapor permeability for both corn zein and gelatin films decreased with the increase of nisin concentration, thus providing a better barrier against water. antimicrobial activity ag ... | 2007 | 18050958 |
| morphological changes induced in listeria monocytogenes v7 by a bacteriocin produced by pediococcus acidilactici. | pediococcus acidilactici produces bacteriocin, which kills listeria monocytogenes. the bactericidal mode of action of the bacteriocin against l. monocytogenes v7 was investigated by transmission electron microscopy. the bacteriocin was purified partially from the cell-free extract using micro-cel and cation-exchange chromatography, and the specific activity was increased 1,791 fold. the bacteriocin (6,400 au/ ml) was inoculated with l. monocytogenes v7 and incubated for 0.5 h, 1 h, 3 h, and 6 h. ... | 2007 | 18051280 |
| production of monoclonal antibody against listeria monocytogenes and its application to immunochromatography strip test. | an immunochromatography (icg) strip test based on a monoclonal antibody for the rapid detection of l. monocytogenes in meat and processed-meat samples was developed in this study. a monoclonal antibody (mab) specific to l. monocytogenes was produced from cloned hybridoma cells (fklm-3b12-37) and used to develop an icg strip test. the antibody showed a stronger binding to l. monocytogenes than other listeria species, and a weak cross-reaction to s. aureus based on an elisa. the detection limit of ... | 2007 | 18051327 |
| ft-ir microspectroscopy: a promising method for the rapid identification of listeria species. | this work presents a pilot study to investigate the potential of fourier transform infrared (ft-ir) microspectroscopy for rapid identification of listeria at the species level. using this technique, ft-ir spectra were acquired from 30 strains from five listeria species. the ft-ir spectra were analysed using stepwise canonical discriminant analysis and partial least-squares regression in a stepwise identification scheme. the results showed that 93% of all the samples were assigned to the correct ... | 2008 | 18053065 |
| effects of sub-lethal concentrations of hexanal and 2-(e)-hexenal on membrane fatty acid composition and volatile compounds of listeria monocytogenes, staphylococcus aureus, salmonella enteritidis and escherichia coli. | in recent years, the interest in the possible use of natural alternatives to food additives to prevent bacterial and fungal growth has notably increased. plants and plant products can represent a source of natural alternatives to improve the shelf-life and the safety of food. some of these compounds, i.e. hexanal, hexanol, 2-(e)-hexenal and 3-(z)-hexenol, produced throughout the lipoxygenase pathway have important roles in plant defence with a protective action towards microbial proliferation in ... | 2008 | 18055050 |
| development of a mast cell-based biosensor. | a mast cell-based biosensor has been developed to enable the use of these cells in numerous applications including pharmaceutical screening, environmental monitoring, clinical diagnosis and homeland security. rat basophilic leukemia (rbl) mast cells offer excellent potential for biosensor applications because they are robust and undergo a dramatic exocytotic response within minutes of antigen addition. to monitor mast cell activation, fluorescent dyes were loaded into the cells and used as indic ... | 2008 | 18055192 |
| listeria monocytogenes desensitizes immune cells to subsequent ca2+ signaling via listeriolysin o-induced depletion of intracellular ca2+ stores. | listeriolysin o (llo), the pore-forming toxin of listeria monocytogenes, is a prototype of the cholesterol-dependent cytolysins (cdcs) secreted by several pathogenic and nonpathogenic gram-positive bacteria. in addition to mediating the escape of the bacterium into the cytosol, this toxin is generally believed to be a central player in host-pathogen interactions during l. monocytogenes infection. llo triggers the influx of ca(2+) into host cells as well as the release of ca(2+) from intracellula ... | 2008 | 18056478 |
| detection, quantification and vitality of listeria monocytogenes in food as determined by quantitative pcr. | in this paper we describe the development of a quantitative pcr (qpcr) technique to detect, quantify and determine the vitality of listeria monocytogenes in foods. the method was based on the amplification of the intergenic region spacer (igs) between the 16s and 23s rrna genes. a panel of more than 100 strains of listeria spp. and non-listeria was used in order to verify the specificity of the primers and taqman probe and amplification signals were obtained only when l. monocytogenes dna and rn ... | 2008 | 18061295 |
| a deterministic model for the processing and presentation of bacteria-derived antigenic peptides. | the amount and the dynamics of antigen supply to the cellular antigen processing and presentation machinery differ largely among diverse microbial antigens and various types of antigen presenting cells. the precise influence, however, of antigen supply on the antigen presentation pattern of cells is not known. here, we provide a basic deterministic mathematical model of antigen processing and presentation of microbial antigens. the model predicts that different types of antigen presenting cells ... | 2008 | 18061621 |
| proteomic analyses of a listeria monocytogenes mutant lacking sigmab identify new components of the sigmab regulon and highlight a role for sigmab in the utilization of glycerol. | in listeria monocytogenes the alternative sigma factor sigmab plays important roles in both virulence and stress tolerance. in this study a proteomic approach was used to define components of the sigmab regulon in l. monocytogenes 10403s (serotype 1/2a). using two-dimensional gel electrophoresis and the recently developed isobaric tags for relative and absolute quantitation technique, the protein expression profiles of the wild type and an isogenic delta sigb deletion strain were compared. overa ... | 2008 | 18065622 |
| inhibition of toll-like receptor signaling in primary murine microglia. | microglial cells respond to the herpes simplex virus (hsv)-1 by producing proinflammatory cytokines and chemokines. after this inflammatory burst, these cells undergo apoptotic cell death. we have recently demonstrated that both virus-induced immune mediator production and apoptosis were induced through toll-like receptor 2 (tlr2) signaling. based upon these findings, we hypothesized that the inhibition of tlr2 signaling may serve as a means to alleviate excessive neuroinflammation. in the prese ... | 2008 | 18066668 |
| a bacterial pore-forming toxin forms aggregates in cells that resemble those associated with neurodegenerative diseases. | listeria monocytogenes is a bacterial, facultative intracellular pathogen, which secretes a pore-forming toxin called listeriolysin o (llo). llo mediates the dissolution of the phagosomal membrane allowing l. monocytogenes to reach and grow in the host cytosolic compartment. in this study we report the localization of llo secreted in infected cells. we described that llo (i) forms small perinuclear aggregates, (ii) accumulates in large autophagosome-like structures and (iii) sequesters to large ... | 2008 | 18067608 |
| inhibition of listeria monocytogenes in dairy products using the bacteriocin-like peptide cerein 8a. | the efficacy of the antimicrobial peptide cerein 8a to control the development of listeria monocytogenes in milk and soft cheese was investigated. the addition of 160 au ml(-1) cerein 8a to uht milk resulted in a decrease of 3 log cycles in viable cells within the 14-day period at 4 degrees c. the viable counts of l. monocytogenes in pasteurized milk samples containing cerein 8a was lower than those observed in controls without bacteriocin. addition of cerein 8a to minas-type soft cheese caused ... | 2008 | 18068253 |
| a significant role for sigma b in the detergent stress response of listeria monocytogenes. | to investigate the contribution of the alternative sigma factor sigma b to detergent stress in listeria monocytogenes upon exposure to the surface-active agents, benzalkoniumchloride (bc), cetylpyridinium chloride (cpc) and sodium docecyl sulfate (sds). | 2008 | 18069984 |
| high-throughput sequence alignment using graphics processing units. | the recent availability of new, less expensive high-throughput dna sequencing technologies has yielded a dramatic increase in the volume of sequence data that must be analyzed. these data are being generated for several purposes, including genotyping, genome resequencing, metagenomics, and de novo genome assembly projects. sequence alignment programs such as mummer have proven essential for analysis of these data, but researchers will need ever faster, high-throughput alignment tools running on ... | 2007 | 18070356 |
| dose-response model for listeria monocytogenes-induced stillbirths in nonhuman primates. | a dose-response model using rhesus monkeys as a surrogate for pregnant women indicates that oral exposure to 10(7) cfu of listeria monocytogenes results in about 50% stillbirths. ten of 33 pregnant rhesus monkeys exposed orally to a single dose of 10(2) to 10(10) cfu of l. monocytogenes had stillbirths. a log-logistic model predicts a dose affecting 50% of animals at 10(7) cfu, comparable to an estimated 10(6) cfu based on an outbreak among pregnant women but much less than the extrapolated esti ... | 2008 | 18070908 |
| foodborne zoonoses due to meat: a quantitative approach for a comparative risk assessment applied to pig slaughtering in europe. | foodborne zoonoses have a major health impact in industrialised countries. new european food safety regulations were issued to apply risk analysis to the food chain. the severity of foodborne zoonoses and the exposure of humans to biological hazards transmitted by food must be assessed. for meat, inspection at the slaughterhouse is historically the main means of control to protect consumers. however, the levels of detection of biological hazards during meat inspection have not been established i ... | 2008 | 18073088 |
| mapping quantitative trait loci from a single-tail sample of the phenotype distribution including survival data. | a new effective bayesian quantitative trait locus (qtl) mapping approach for the analysis of single-tail selected samples of the phenotype distribution is presented. the approach extends the affected-only tests to single-tail sampling with quantitative traits such as the log-normal survival time or censored/selected traits. a great benefit of the approach is that it enables the utilization of multiple-qtl models, is easy to incorporate into different data designs (experimental and outbred popula ... | 2007 | 18073434 |
| effect of relative inoculum concentration on listeria monocytogenes growth in co-culture. | growth suppression of multi-species bacterial populations in batch cultures by a single 'dominant' strain has been referred to in the literature as the 'jameson effect'. the effect is often attributed to production of specific inhibitors of growth by one species against another. to explore its basis, we hypothesised that the jameson effect is often a non-specific interaction and that growth inhibition of species in co-culture can be controlled by manipulation of inoculum concentration and growth ... | 2008 | 18083261 |
| activation of cytosolic phospholipase a2alpha in resident peritoneal macrophages by listeria monocytogenes involves listeriolysin o and tlr2. | eicosanoid production by macrophages is an early response to microbial infection that promotes acute inflammation. the intracellular pathogen listeria monocytogenes stimulates arachidonic acid release and eicosanoid production from resident mouse peritoneal macrophages through activation of group iva cytosolic phospholipase a2 (cpla2alpha). the ability of wild type l. monocytogenes (wtlm) to stimulate arachidonic acid release is partially dependent on the virulence factor listeriolysin o; howeve ... | 2008 | 18083708 |
| post-colonoscopic listeria septicemia in ulcerative colitis during immunosuppressive therapy. | a 78-year-old man who had been diagnosed with ulcerative colitis was admitted because of uncontrolled severe, frequent, bloody diarrhea. he was treated with immunosuppressive therapy that included corticosteroid and azathioprine. colonoscopy was used to assess disease activity. this revealed that the mucosa of his digestive tract from the rectum to the ileum was damaged. he developed a high-grade fever soon after colonoscopy. blood culture demonstrated listeria monocytogenes. treatment was chang ... | 2007 | 18084128 |
| transient transfection of echinococcus multilocularis primary cells and complete in vitro regeneration of metacestode vesicles. | a major limitation in studying molecular interactions between parasitic helminths and their hosts is the lack of suitable in vitro cultivation systems for helminth cells and larvae. here we present a method for long-term in vitro cultivation of larval cells of the tapeworm echinococcus multilocularis, the causative agent of alveolar echinococcosis. primary cells isolated from cultivated metacestode vesicles in vitro showed a morphology typical of echinococcus germinal cells, displayed an echinoc ... | 2008 | 18086473 |
| use of nisin-coated plastic films to control listeria monocytogenes on vacuum-packaged cold-smoked salmon. | cold-smoked (salmo salar) salmon samples were surface-inoculated with a cocktail of three nisin-resistant strains of l. monocytogenes (psu1, psu2 and psu21) to a level of approximately 5 x 10(2) or 5 x 10(5) cfu/cm2 of salmon surface. the inoculated smoked salmon samples were vacuum-packaged with control film (no nisin) or nisin-coated plastic films and stored at either 4 or 10 degrees c. when the inoculated smoked salmon samples were packaged with film coated with 2000 iu/cm2 of nisin, a reduct ... | 2008 | 18086503 |
| antigen delivered by anthrax lethal toxin induces the development of memory cd8+ t cells that can be rapidly boosted and display effector functions. | memory cd8+ t cells are essential for protective immunity against many intracellular pathogens; therefore, stimulation of this population of cells is an important goal of vaccination. we have previously shown that a detoxified derivative of bacillus anthracis anthrax lethal toxin (lt) can deliver heterologous cd8+ t-cell epitopes to the major histocompatibility complex class i processing and presentation pathway of murine host cells and that immunization of mice with these lt-antigen fusion prot ... | 2008 | 18086810 |
| isolation and purification of enterocin e-760 with broad antimicrobial activity against gram-positive and gram-negative bacteria. | strain nrrl b-30745, isolated from chicken ceca and identified as enterococcus durans, enterococcus faecium, or enterococcus hirae, was initially identified as antagonistic to campylobacter jejuni. the isolate produced a 5,362-da bacteriocin (enterocin) that inhibits the growth of salmonella enterica serovar enteritidis, s. enterica serovar choleraesuis, s. enterica serovar typhimurium, s. enterica serovar gallinarum, escherichia coli o157:h7, yersinia enterocolitica, citrobacter freundii, klebs ... | 2008 | 18086839 |
| characterization of a listeria monocytogenes protein interfering with rab5a. | listeria monocytogenes (lm) phagocytic strategy implies recruitment and inhibition of rab5a. here, we identify a listeria protein that binds to rab5a and is responsible for rab5a recruitment to phagosomes and impairment of the gdp/gtp exchange activity. this protein was identified as a glyceraldehyde-3-phosphate dehydrogenase (gapdh) from listeria (p40 protein, lmo 2459). the p40 protein was found within the phagosomal membrane. analysis of the sequence of lm p40 protein revealed two enzymatic d ... | 2008 | 18088303 |
| effect of inhibitory extracts derived from liquid smoke combined with postprocess pasteurization for control of listeria monocytogenes on ready-to-eat meats. | surface pasteurization was examined in combination with low-phenolic antimicrobial extracts derived from liquid smoke to inhibit and prevent the growth of listeria monocytogenes during the shelf life of ready-to-eat meats. in preliminary trials with retail frankfurters, one smoke derivative (2-min dip) produced a 0.3-log reduction of l. monocytogenes and a 1-min in-bag pasteurization (73.9 degrees c) produced a 2.9-log reduction, whereas a combination of the two treatments produced a 5.3-log red ... | 2007 | 18095426 |
| antimicrobial activity of clove and cinnamon essential oils against listeria monocytogenes in pasteurized milk. | the antimicrobial activity of essential oils (eos) of cinnamon bark, cinnamon leaf, and clove against listeria monocytogenes scott a were studied in semiskimmed milk incubated at 7 degrees c for 14 days and at 35 degrees c for 24 h. the mic was 500 ppm for cinnamon bark eo and 3,000 ppm for the cinnamon leaf and clove eos. these effective concentrations increased to 1,000 ppm for cinnamon bark eo, 3,500 ppm for clove eo, and 4,000 ppm for cinnamon leaf eo when the semiskimmed milk was incubated ... | 2007 | 18095427 |
| pulsed-plasma gas-discharge inactivation of microbial pathogens in chilled poultry wash water. | a pulsed-plasma gas-discharge (ppgd) system was developed for the novel decontamination of chilled poultry wash water. treatment of poultry wash water in the plasma generation chamber for up to 24 s at 4 degrees c reduced escherichia coli nctc 9001, campylobacter jejuni atcc 33560, campylobacter coli atcc 33559, listeria monocytogenes nctc 9863, salmonella enterica serovar enteritidis atcc 4931, and s. enterica serovar typhimurium atcc 14028 populations to non-detectable levels (< or = 8 log cfu ... | 2007 | 18095434 |
| comparing uncertainty resulting from two-step and global regression procedures applied to microbial growth models. | two different microbial modeling procedures were compared and validated against independent data for listeria monocytogenes growth. the most generally used method is two consecutive regressions: growth parameters are estimated from a primary regression of microbial counts, and a secondary regression relates the growth parameters to experimental conditions. a global regression is an alternative method in which the primary and secondary models are combined, giving a direct relationship between exp ... | 2007 | 18095435 |
| different enrichment procedures for recovery of listeria monocytogenes from raw chicken samples can affect the results of detection (by chromogenic plating or real-time pcr) and lineage or strain identification. | this study aimed to evaluate the effect of different enrichment procedures on the detection of listeria monocytogenes in food, by a comparison of subculture onto chromogenic agar with real-time pcr. two different culture media, the primary and secondary enrichment broths of the u.s. food safety and inspection service (fsis) method used for pcr detection of l. monocytogenes, were compared for the primary enrichment of retail ground chicken samples. l. monocytogenes was detected after the completi ... | 2007 | 18095442 |
| bacterial contamination of sago starch in papua new guinea. | sago starch is an important food in lowland papua new guinea. extraction of the starch from the palm and storage were performed by way of traditional methods that have been used for thousands of years. currently, very little is known about the microbiology of sago starch. sago samples were collected from areas of high starch utilization and analyzed for the presence of bacterial pathogens and indicator organisms. storage methods and duration were recorded at the time of collection, and ph and wa ... | 2007 | 18095446 |
| cleaning and handling implements as potential reservoirs for bacterial contamination of some ready-to-eat foods in retail delicatessen environments. | this study assessed the association of bacteria with cleaning tools, such as floor mops (n = 25) and cleaning cloths (n = 39), and handling devices, such as disposable plastic gloves (n = 20), used during filled baguette and assorted salad preparation in four selected retail delicatessens in johannesburg, south africa. samples of each cleaning or handling tool were prepared for aerobic (apc), coliform (cc), escherichia coli (ec), bacillus cereus (bcc), and staphylococcus aureus (sac) counts, as ... | 2007 | 18095448 |
| multiple-locus variable-number tandem-repeats analysis of listeria monocytogenes using multicolour capillary electrophoresis and comparison with pulsed-field gel electrophoresis typing. | the multiple-locus variable-number tandem-repeats analysis (mlva) method for genotyping has proven to be a fast and reliable typing tool in several bacterial species. mlva is in our laboratory the routine typing method for salmonella enterica subsp. enterica serovar typhimurium and escherichia coli o157. the gram-positive bacteria listeria monocytogenes, while not isolated as frequent as s. typhimurium and e. coli, causes severe illness with an overall mortality rate of 30%. thus, it is importan ... | 2008 | 18096258 |
| sequential exposure to carbon nanotubes and bacteria enhances pulmonary inflammation and infectivity. | carbon nanotubes (cnt), with their applications in industry and medicine, may lead to new risks to human health. cnt induce a robust pulmonary inflammation and oxidative stress in rodents. realistic exposures to cnt may occur in conjunction with other pathogenic impacts (microbial infections) and trigger enhanced responses. we evaluated interactions between pharyngeal aspiration of single-walled cnt (swcnt) and bacterial pulmonary infection of c57bl/6 mice with listeria monocytogenes (lm). mice ... | 2008 | 18096873 |
| antimicrobial activity of nisin, reuterin, and the lactoperoxidase system on listeria monocytogenes and staphylococcus aureus in cuajada, a semisolid dairy product manufactured in spain. | the inhibitory activity of nisin (n), reuterin (r), and the lactoperoxidase system (lps), added individually or in combination, against listeria monocytogenes and staphylococcus aureus was investigated in "cuajada" (curdled milk), a semisolid dairy product manufactured in spain. cuajada was manufactured from uht skim milk separately inoculated with l. monocytogenes and staph. aureus, each at approximately 4 log cfu/ml, and held under conditions of temperature abuse (10 degrees c). on d 3, a syne ... | 2008 | 18096926 |
| studies on listeria monocytogenes; isolation of a monocytosis-producing agent. | | 1949 | 18127671 |
| studies on listeria monocytogenes; role of listeria in infectious mononucleosis. | | 1949 | 18127672 |
| use of results of microbiological analyses for risk-based control of listeria monocytogenes in marinated broiler legs. | microbial risk assessment provides a means of estimating consumer risks associated with food products. the methods can also be applied at the plant level. in this study results of microbiological analyses were used to develop a robust single plant level risk assessment. furthermore, the prevalence and numbers of listeria monocytogenes in marinated broiler legs in finland were estimated. these estimates were based on information on the prevalence, numbers and genotypes of l. monocytogenes in 186 ... | 2008 | 18155311 |
| ammonia disinfection of animal feeds --laboratory study. | animal feeds may be contaminated, accidentally or maliciously, with a number of zoonotic bacteria. animal infections with these bacterial agents, whether or not they cause animal disease, may lead to human illnesses. anhydrous ammonia was introduced on farms in developed countries as a high-nitrogen soil amendment, but later found use in enhancing crude protein in low-quality roughage fed to ruminants and in neutralizing mycotoxins in fungus-infested feed grains. although ammonia has been known ... | 2008 | 18155794 |
| the inhibitory spectrum of thermophilin 9 from streptococcus thermophilus lmd-9 depends on the production of multiple peptides and the activity of blpg(st), a thiol-disulfide oxidase. | the blp(st) cluster of streptococcus thermophilus lmd-9 was recently shown to contain all the genetic information required for the production of bacteriocins active against other s. thermophilus strains. in this study, we further investigated the antimicrobial activity of s. thermophilus lmd-9 by testing the susceptibility of 31 bacterial species (87 strains). we showed that lmd-9 displays an inhibitory spectrum targeted toward related gram-positive bacteria, including pathogens such as listeria ... | 2008 | 18156339 |
| folding thermodynamics and kinetics of the leucine-rich repeat domain of the virulence factor internalin b. | although the folding of alpha-helical repeat proteins has been well characterized, much less is known about the folding of repeat proteins containing beta-sheets. here we investigate the folding thermodynamics and kinetics of the leucine-rich repeat (lrr) domain of internalin b (inlb), an extracellular virulence factor from the bacterium lysteria monocytogenes. this domain contains seven tandem leucine-rich repeats, of which each contribute a single beta-strand that forms a continuous beta-sheet ... | 2008 | 18156467 |
| listeria meningitis complicating infliximab treatment for crohn's disease. | infliximab, a monoclonal antibody directed against tumour necrosis factor-alpha, is an effective therapy for crohn's disease. though uncommon, serious opportunistic infections, including reactivation of tuberculosis, have occurred in patients after infliximab administration.meningitis caused by listeria monocytogenes developed in a 37-year-old man six days after the second infusion of infliximab. the patient, who also was treated with azathioprine and corticosteroids, had an uneventful recovery ... | 2005 | 18159561 |
| involvement of cell fatty acid composition and lipid metabolism in adhesion mechanism of listeria monocytogenes. | the cell fatty acid (fa) composition of adhered and floating cells of two listeria monocytogenes strains, selected on the basis of the biofilm forming ability, was taken into consideration. anteiso-15:0, iso-c15:0 and anteiso-17:0 resulted the principal fas in floating cells. on the other hand the adhered cells of the two strains presented a fas profile characterized by a prevalence of straight fas such as hexadecanoic acid (16:0) and octadecanoic acid (18:0). when adhered and free floating cell ... | 2008 | 18160165 |
| the proteasome immunosubunit multicatalytic endopeptidase complex-like 1 is a t-cell-intrinsic factor influencing homeostatic expansion. | homeostatic regulatory mechanisms maintain the constant ratios between different lymphocyte subsets in the secondary lymphoid organs. how this dynamic equilibrium is achieved, in particular following the clonal expansion and subsequent contraction of different cells after infection, remains poorly understood. expression of the proteasome immunosubunits has been shown to influence not only major histocompatibility complex class i (mhc-i) antigen processing and thereby t-cell responses, but also t ... | 2008 | 18160473 |
| card6 is interferon inducible but not involved in nucleotide-binding oligomerization domain protein signaling leading to nf-kappab activation. | we have previously reported the cloning and characterization of card6, a caspase recruitment domain (card)-containing protein that is structurally related to the interferon (ifn)-inducible gtpases. card6 associates with microtubules and with receptor-interacting protein 2 (rip2). rip2 mediates nf-kappab activation induced by the intracellular nucleotide-binding oligomerization domain (nod) receptors that sense bacterial peptidoglycan. here we report that the expression of card6 and rip2 in bone ... | 2008 | 18160713 |
| early invasive listeria monocytogenes infection after orthotopic liver transplantation: case report and review of the literature. | infection with listeria monocytogenes is rare, with a reported annual incidence of 4.4 cases per million individuals. it is caused by a gram-positive rod-shaped bacterium (listeria monocytogenes) that can be found in soil, vegetation, water, sewage, and silage and in feces of humans and animals. it is a facultative intracellular pathogen with the ability to survive and multiply in phagocytic host cells, even in adverse environmental circumstances. listeriosis has rarely been reported after ortho ... | 2008 | 18161771 |
| preparation of listeria monocytogenes specimens for molecular detection and identification. | listeria monocytogenes is a common foodborne pathogen that has the capacity to cause severe clinical illness in vulnerable human population groups. the availability of rapid and specific laboratory tests to identify this bacterium is essential for preventing an otherwise easily treated malaise from developing into a life-threatening disease. to this end, a variety of rapid, sensitive and precise nucleic acid-based assays have been developed, contributing to the improved diagnosis of listeriosis. ... | 2008 | 18164775 |
| radical scavenging, antioxidant and antimicrobial activities of halophytic species. | for the first time, both antioxidant and antimicrobial activities are simultaneously reported in halophytic plants, particularly on polar fractions. chloroformic and methanolic extracts of the halophytes eryngium maritimum l., crithmum maritimum l. and cakile maritima scop. were tested for their antimicrobial activities against 12 bacterial and yeast strains. in addition, radical scavenging and antioxidant activities were assessed, as well as total phenol contents. only one bacterial strain (lis ... | 2008 | 18164885 |
| screening of the enterocin genes and antimicrobial activity against pathogenic bacteria in enterococcus strains obtained from different origins. | antimicrobial activities of 139 enterococcus isolates (48 e. faecium and 91 e. faecalis) obtained from canine feces, boiler meat samples, swine feces, wild waterfowl feces, and human feces were examined against respective bacteria, including streptococcus pyogenes, staphylococcus aureus, bacillus subtilis, listeria monocytogenes, salmonella enteritidis, and escherichia coli. bacteriocin (bac) production assay revealed that the antimicrobial activity against at least one of 6 indicator strains (b ... | 2007 | 18176018 |
| acid and heat tolerance of persistent and nonpersistent listeria monocytogenes food plant strains. | acid and heat tolerance of 17 persistent and 23 nonpersistent listeria monocytogenes strains, recovered from three meat-processing plants, were investigated. | 2008 | 18179448 |
| developmental switch of intestinal antimicrobial peptide expression. | paneth cell-derived enteric antimicrobial peptides provide protection from intestinal infection and maintenance of enteric homeostasis. paneth cells, however, evolve only after the neonatal period, and the antimicrobial mechanisms that protect the newborn intestine are ill defined. using quantitative reverse transcription-polymerase chain reaction, immunohistology, reverse-phase high-performance liquid chromatography, and mass spectrometry, we analyzed the antimicrobial repertoire in intestinal ... | 2008 | 18180308 |
| type i ifn are host modulators of strain-specific listeria monocytogenes virulence. | type i ifn (ifn-i) increase the sensitivity of cells and mice to lethal infection with listeria monocytogenes. therefore the amount of ifn-i produced during infection might be an important factor determining listeria virulence. two commonly used strains of l. monocytogenes, egd and lo28, were identified as, respectively, low and high inducers of ifn-i synthesis in infected macrophages. increased ifn-i production resulted from the stronger ability of the lo28 strain to trigger the irf3 signalling ... | 2008 | 18182083 |
| quantification of bacterial cells based on autofluorescence on a microfluidic platform. | bacterial counts provide important information during the processes such as pathogen detection and hygiene inspection and these processes are critical for public health and food/pharmaceutical production. in this study, we demonstrate the quantification of the number of bacterial cells based on the autofluorescence from the cell lysate on a microfluidic chip. we tested three model pathogenic bacteria (listeria monocytogenes f4244, salmonella enteritidis pt1 and escherichia coli o157:h7 edl 933). ... | 2008 | 18187141 |
| in vitro efficacy of diclofenac against listeria monocytogenes. | chemotherapy is often futile in systemic listeriosis, translating to being a peril to public health. there is, thus, an imperative need for novel antilisterial compounds, possibly acting through mechanisms dissimilar to those of existing drugs. the present study describes one such agent-the non-steroidal anti-inflammatory drug (nsaid) diclofenac sodium (dc). the national committee for clinical laboratory standards (nccls) minimum inhibitory concentration (mic), mode of action, and two mechanisms ... | 2008 | 18188616 |
| rage: exacting a toll on the host in response to polymicrobial sepsis and listeria monocytogenes. | the receptor for advanced glycation endproducts (rage) has complex roles in the immune/inflammatory response. rage is expressed on monocytes/macrophages, t and b lymphocytes, and dendritic cells. previous studies illustrated that homozygous rage-/- mice subjected to overwhelming bacterial sepsis displayed normal clearance of pathogenic bacteria and significantly increased survival. in this issue of critical care, lutterloh and colleagues confirm these findings and provide evidence that blocking ... | 2007 | 18190725 |
| bioprotection of golden delicious apples and iceberg lettuce against foodborne bacterial pathogens by lactic acid bacteria. | lactic acid bacteria were isolated from fresh vegetables and fruit and its ability to inhibit the growth of foodborne human pathogens (escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, and staphylococcus aureus) was tested using the agar spot assay. eighteen isolates showed a strong antagonistic capacity and were further characterised and identified using 16s rdna sequencing and api 50ch. most of them pertained to leuconostoc spp. and lactobacillus plantar ... | 2008 | 18191266 |
| teichoic acid glycosylation mediated by gtca is required for phage adsorption and susceptibility of listeria monocytogenes serotype 4b. | an insertion mutant of gtca, responsible for serotype-specific glycosylation of the cell wall teichoic acid in serotype 4b strains of listeria monocytogenes, was also resistant to both listeria genus- and serotype 4b-specific phages. the sugar substituents on teichoic acid appeared essential for the adsorption of phages a500 (serotype 4b specific) and a511 (listeria genus specific) to serotype 4b l. monocytogenes. | 2008 | 18192405 |
| heavy-metal and benzalkonium chloride resistance of listeria monocytogenes isolates from the environment of turkey-processing plants. | the resistance of listeria monocytogenes to cadmium and arsenic has been used extensively for strain subtyping. however, limited information is available on the prevalence of such resistance among isolates from the environment of food-processing plants. in addition, it is not known whether the resistance of such isolates to heavy metals may correlate with resistance to quaternary ammonium compounds extensively used as disinfectants in the food-processing industry. in this study, we characterized ... | 2008 | 18192428 |
| distinct tlr- and nlr-mediated transcriptional responses to an intracellular pathogen. | how the innate immune system tailors specific responses to diverse microbial infections is not well understood. cells use a limited number of host receptors and signaling pathways to both discriminate among extracellular and intracellular microbes, and also to generate responses commensurate to each threat. here, we have addressed these questions by using dna microarrays to monitor the macrophage transcriptional response to the intracellular bacterial pathogen listeria monocytogenes. by utilizin ... | 2008 | 18193943 |
| influence of temperature on biofilm formation by listeria monocytogenes on various food-contact surfaces: relationship with motility and cell surface hydrophobicity. | to assess the ability of listeria monocytogenes to form biofilm on different food-contact surfaces with regard to different temperatures, cellular hydrophobicity and motility. | 2008 | 18194252 |
| crosstalk between the alpha2beta1 integrin and c-met/hgf-r regulates innate immunity. | data from several investigators suggest that the alpha2beta1 integrin, a receptor for collagens, laminins, decorin, e-cadherin, matrix metalloproteinase-1, endorepellin, and several viruses, is required for innate immunity and regulation of autoimmune/allergic disorders. we demonstrated that the innate immune response to listeria monocytogenes required alpha2beta1 integrin expression by peritoneal mast cells (pmcs). ligation of the alpha2beta1 integrin by c1q contained in immune complexes compri ... | 2008 | 18198349 |
| listeriolysin o allows listeria monocytogenes replication in macrophage vacuoles. | listeria monocytogenes is an intracellular bacterial pathogen that replicates rapidly in the cytosol of host cells during acute infection. surprisingly, these bacteria were found to occupy vacuoles in liver granuloma macrophages during persistent infection of severe combined immunodeficient (scid) mice. here we show that l. monocytogenes can replicate in vacuoles within macrophages. in livers of scid mice infected for 21 days, we observed bacteria in large lamp1(+) compartments that we termed sp ... | 2008 | 18202661 |
| isolation of lactic acid bacteria with inhibitory activity against pathogens and spoilage organisms associated with fresh meat. | the use of lactic acid bacteria (lab) as protective cultures in vacuum-packed chill-stored meat has potential application for assuring and improving food quality, safety and market access. in a study to identify candidate strains suitable for evaluation in a meat model, agar-based methods were employed to screen 181 chilled meat and meat process-related lab for strains inhibitory to pathogens and spoilage organisms of importance to the meat industry. six meat-derived strains, including lactobaci ... | 2008 | 18206764 |
| control of listeria monocytogenes on ham steaks by antimicrobials incorporated into chitosan-coated plastic films. | contamination of ready-to-eat (rte) meat products such as ham steaks with listeria monocytogenes has been a concern for the meat processing industry. the objective of this study was to evaluate the antilisterial efficacy of chitosan-coated plastic films alone or incorporating five generally recognized as safe (gras) antimicrobials. effect of chitosan-coated plastic film on the growth of l. monocytogenes was first investigated in an aqueous system of culture medium broth and chitosan-coated films ... | 2008 | 18206768 |
| succession of dominant and antagonistic lactic acid bacteria in fermented cucumber: insights from a pcr-based approach. | the goal of the investigation was to study the succession of major groups of lactic acid bacteria (lab) and their antagonism in salt-fermented cucumber using pcr. in a direct detection method as well as a short enrichment process, pcr enabled detection of leuconostoc and lactobacillus during early hours of fermentation. subsequently, lactobacillus and pediococcus emerged as the dominant genera. nucleic acid sequence of culture-independent clones confirmed the detection of pediococcus as a domina ... | 2008 | 18206770 |
| use of mild-heat treatment following high-pressure processing to prevent recovery of pressure-injured listeria monocytogenes in milk. | this study examined the inactivation of listeria monocytogenes in milk by high-pressure processing (hpp) and bacterial recovery during storage after hpp. we developed a technique to inhibit the bacterial recovery during storage after hpp (550 mpa for 5 min) using a mild-heat treatment (30-50 degrees c). various mild-heat treatments were conducted following hpp to investigate the condition on which the bacterial recovery was prevented. immediately after hpp of 550 mpa at 25 degrees c for 5 min, n ... | 2008 | 18206771 |
| heat and acid tolerance of listeria monocytogenes after exposure to single and multiple sublethal stresses. | the majority of published studies on the adaptive heat or acid tolerance response of listeria monocytogenes have been performed with a single strain exposed to a single adaptation treatment; however, in food ecosystems, microorganisms commonly exist as multi-species communities and encounter multiple stresses, which may result in "stress hardening". therefore, the present study evaluated the adaptive responses to heat (52, 57 and 63 degrees c) or lactic acid (ph 3.5) of a 10-strain composite of ... | 2008 | 18206772 |
| microbiological quality of retail cheeses made from raw, thermized or pasteurized milk in the uk. | two studies of retail fresh, ripened and semi-hard cheeses made from raw, thermized or pasteurized milk were undertaken in the uk during 2004 and 2005 to determine the microbiological quality of these products. using microbiological criteria in european commission recommendations 2004/24/ec and 2005/175/ec, 2% of both raw, thermized (37/1819 samples) and pasteurized (51/2618 samples) milk cheeses were of unsatisfactory quality. raw or thermized milk cheeses were of unsatisfactory quality due to ... | 2008 | 18206773 |
| microbial ecology of food contact surfaces and products of small-scale facilities producing traditional sausages. | the microbial status in 7 small-scale facilities (ssfs) producing traditional fermented and/or dry sausages was investigated. it was shown that the hygienic status of the processing environment and equipment plays an essential role in the microbial stability and safety of the final products. the current study revealed that the majority of the sampling sites (control points) tested were highly (>4 log cfu/cm(2)) contaminated by spoilage flora (i.e. pseudomonas, enterobacteriaceae), with knives, t ... | 2008 | 18206774 |
| enumeration and growth of naturally occurring listeria spp. in unpackaged ham. | a total of 301 unpackaged retail ham samples were tested for the presence and number of listeria spp. after 7 days at 5 degrees c to simulate domestic storage. thirteen samples (4.3%) contained listeria monocytogenes, with the highest count being 1.6 x 10(3)cfu g(-1). thirteen samples contained other listeria spp. genotyping showed that only one l. monocytogenes isolate from the 14 tested was of a type previously identified in new zealand human cases. listeria-contaminated batches were incubated ... | 2008 | 18206784 |
| [arthritis due to listeria monocytogenes]. | a 69-year-old man with liver cirrhosis presented to a surgeon, complaining of pain and swelling involving the right knee joint. a cloudy yellow synovial fluid was aspirated. the examination revealed polymorphonuclear leukocytes and listeria monocytogenes was grown in the culture. the patient was treated with oral cotrimoxazole. further follow-up was uneventful. the literature on listerial joint infections is briefly reviewed. | 2008 | 18210136 |
| elimination of listeria monocytogenes on hotdogs by infrared surface treatment. | the objective of this research was to develop an infrared pasteurization process with automatic temperature control for inactivation of surface-contaminated listeria monocytogenes on ready-to-eat meats such as hotdogs. the pasteurization system contained 4 basic elements: an infrared emitter, a hotdog roller, an infrared sensor, and a temperature controller. the infrared sensor was used to monitor the surface temperature of hotdogs while the infrared emitter, modulated by a power controller, was ... | 2008 | 18211358 |
| avoiding death by autophagy: interactions of listeria monocytogenes with the macrophage autophagy system. | autophagy restricts the growth of a variety of intracellular pathogens. however, cytosol-adapted pathogens have evolved ways to evade restriction by this innate immune mechanism. listeria monocytogenes is a gram-positive bacterial pathogen that utilizes a cholesterol-dependent pore-forming toxin, listeriolysin o (llo), to escape from the phagosome. autophagy targets l. monocytogenes in llo-damaged phagosomes and also in the cytosol under some experimental conditions. however, this bacterium has ... | 2008 | 18216493 |
| disinfectant test against monoculture and mixed-culture biofilms composed of technological, spoilage and pathogenic bacteria: bactericidal effect of essential oil and hydrosol of satureja thymbra and comparison with standard acid-base sanitizers. | to assess the antimicrobial action of three natural-derived products (essential oil, decoction and hydrosol of satureja thymbra) against biofilms, composed of useful, spoilage and pathogenic bacteria (formed as monoculture or/and mixed-culture), and to compare their efficiency with three standard acid and alkaline chemical disinfectants. | 2008 | 18217930 |
| a study on the kinetic behavior of listeria monocytogenes in ice cream stored under static and dynamic chilling and freezing conditions. | the kinetic behavior of listeria monocytogenes in 2 commercial ice cream products (a and b) that were inoculated and stored under static chilling (4 to 16 degrees c), static freezing (-5 to -33 degrees c), dynamic chilling, and dynamic chilling-freezing conditions was studied, simulating conditions of the aging process and of normal or abuse conditions during distribution and storage. the ice cream products a and b had different compositions but similar ph (6.50 and 6.67, respectively) and water ... | 2008 | 18218738 |
| listeria-based anti-infective vaccine strategies. | listeria monocytogenes is a gram-positive intracellular bacterium that has accounted for a significant proportion of human foodborne infections in recent decades. although infection with sublethal level of l. monocytogenes generates enduring immunity, it is impractical to apply intact virulent strains as vaccine. through use of killed, attenuated, naturally avirulent, subcellular and dna vaccine preparations, significant protection has been achieved in experimental animals against listeriosis. b ... | 2006 | 18221153 |
| prevalence of listeria species in food products in isfahan, iran. | a total of 617 meat and meat products, diary, vegetables and ready to eat food samples were collected. listeria spp. isolated by using usda method of isolation and l. monocytogenes identified by biochemical and polymerase chain reaction (pcr). the incidence of listeria spp. was 4.6% in all food samples. l. monocytogenes was found in 1.2% of food samples. it was found that listeria spp. was present in 6.7% of meat and meat product samples, 1.3% of diary samples, 1.2% of vegetable samples and 12% ... | 2008 | 18221811 |
| transcription of the listeria monocytogenes fri gene is growth-phase dependent and is repressed directly by fur, the ferric uptake regulator. | the listeria monocytogenes fri gene encodes the only ferritin-like protein of this pathogen, a dps protein (dna binding protein from starved cells). listeria dps is endowed with the capacity to detoxify concurrently free iron and h(2)o(2), is essential for virulence and is required for efficient bacterial growth at early stages of the infection process. the transcription of fri is known to depend on sigma(a) and sigma(b) factors, to be affected by growth conditions and to be derepressed in a per ... | 2008 | 18222616 |