| antimicrobial activity of extracts of the lichen parmelia sulcata and its salazinic acid constituent. | the antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. all of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, yersinia enterocolitica, staphylococcus aureus ... | 2007 | 17913083 |
| pre-existing anti-salmonella vector immunity prevents the development of protective antigen-specific cd8 t-cell frequencies against murine listeriosis. | our laboratory has focused its research on the use of the type iii secretion system of salmonella enterica serovar typhimurium to translocate heterologous antigens directly into the cytosol of antigen-presenting cells. we have previously reported that the single oral immunization of mice with a recombinant salmonella aroa/sptp mutant strain expressing the translocated yersinia outer protein e fused to the immunodominant antigen p60 from listeria monocytogenes in a type iii-mediated fashion resul ... | 2007 | 17913544 |
| protein kinase c delta is essential for optimal macrophage-mediated phagosomal containment of listeria monocytogenes. | activation of macrophages and subsequent "killing" effector functions against infectious pathogens are essential for the establishment of protective immunity. nf-il6 is a transcription factor downstream of ifn-gamma and tnf in the macrophage activation pathway required for bacterial killing. comparison of microarray expression profiles of listeria monocytogenes (lm)-infected macrophages from wt and nf-il6-deficient mice enabled us to identify candidate genes downstream of nf-il6 involved in the ... | 2007 | 17913887 |
| a novel real-time pcr-based method for the detection of listeria monocytogenes in food. | a new real-time pcr-based method was developed for the detection of listeria monocytogenes in food. | 2007 | 17916129 |
| in situ imaging of the endogenous cd8 t cell response to infection. | mounting a protective immune response is critically dependent on the orchestrated movement of cells within lymphoid organs. we report here the visualization, using major histocompatability complex class i tetramers, of the cd8-positive (cd8) t cell response in the spleens of mice to listeria monocytogenes infection. a multistage pathway was revealed that included initial activation at the borders of the b and t cell zones followed by cluster formation with antigenpresenting cells leading to cd8 ... | 2007 | 17916739 |
| impact of lymphocyte apoptosis on the innate immune stages of infection. | infection of mice with listeria monocytogenes has led to a puzzling observation: mice deficient in lymphocytes are more resistant during the early innate immune response. this is counterintuitive, because mice deficient in the adaptive immune response are unable to clear the infection and eventually die. this work will highlight some of our recent work on listeria induced apoptosis and its immunological consequences. we show that listeria produces a toxin, listeriolysin o (llo), which causes apo ... | 2007 | 17917040 |
| in silico and in vitro characterization of mgbp4 splice variants. | in a systematic approach to identify interferon-gamma (ifn-gamma)-regulated host effector molecules, we found several members of the 65 kda guanylate-binding proteins (gbps) highly upregulated. during extensive characterization of these guanosine triphosphatases (gtpases), we identified discrepancies between the cloned and published sequences of the murine gtpase mgbp4. two splice variants of mgbp4 could be detected. one variant led to a premature stop codon after 312 bp. the second variant resu ... | 2007 | 17919070 |
| the listeria monocytogenes homolog of the escherichia coli era gene is involved in adhesion to inert surfaces. | two transposon-insertional mutants of listeria monocytogenes showing smaller viable surface-attached cell populations after disinfection with n,n-didecyl-n,n-dimethylammonium chloride were identified. in both mutants, transposon tn917-lac was found to be inserted into the same gene, lmo1462, which is homologous to the essential escherichia coli era gene. both l. monocytogenes lmo1462-disrupted mutants displayed lower growth rates, as was also shown for several e. coli era mutants, and the lmo146 ... | 2007 | 17921262 |
| the non-canonical role of atg family members as suppressors of innate antiviral immune signaling. | recent research on autophagy clearly demonstrates that the autophagosome-lysosome pathway plays essential roles in elimination of certain pathogens such as group a streptococcus, mycobacterium tuberculosis, listeria monocytogenes, and herpesvirus. (1-4) we have recently found that a key regulator of the autophagic process, the atg12-atg5 conjugate, associates with the signaling molecules retinoic acid-inducible gene i (rig-i) and interferon-beta promoter stimulator 1 (ips-1), which are essential ... | 2008 | 17921696 |
| improved solubilization of surface proteins from listeria monocytogenes for 2-de. | solubilization of bacterial surface (cell wall and membrane-associated) proteins for 2-de is challenging, particularly in the case of gram-positive bacteria. this is primarily due to strong protein association with the cell wall peptidoglycan and protein hydrophobicity. we solubilized surface proteins for 2-de from the gram-positive pathogen listeria monocytogenes using mutanolysin, which digests cell wall peptidoglycan, and one of three different mixtures of zwitterionic detergent and chaotrope ... | 2007 | 17922522 |
| purification and identification of the pediocin produced by pediococcus acidilactici mm33, a new human intestinal strain. | the aim of this study was to purify and identify the bacteriocin produced by pediococcus acidilactici mm33, a strain previously isolated from human gut. | 2008 | 17927747 |
| differential expression of inlb and acta in listeria monocytogenes in selective and nonselective enrichment broths. | to investigate the effect of selective and nonselective media on the expression of acta and inlb proteins in listeria monocytogenes. | 2008 | 17927754 |
| rapid diagnosis of experimental meningitis by bacterial heat production in cerebrospinal fluid. | calorimetry is a nonspecific technique which allows direct measurement of heat generated by biological processes in the living cell. we evaluated the potential of calorimetry for rapid detection of bacterial growth in cerebrospinal fluid (csf) in a rat model of bacterial meningitis. | 2007 | 17927816 |
| rapid methods to assess sanitizing efficacy of benzalkonium chloride to listeria monocytogenes biofilms. | different methods were used to investigate biofilm growth including crystal violet staining, atp bioluminescence and total viable count. seven strains of listeria monocytogenes and 8 of their derivative strains were screened for their capacity to form biofilms. both adaptation to benzalkonium chloride (bc) and curing of plasmids did not significantly affect biofilm-forming ability. the strains of l. monocytogenes belonging to serotype 1 formed biofilms significantly better as compared to serotyp ... | 2007 | 17928079 |
| [first experience with detecting bacterial dna in the cerebrospinal fluid of patients with purulent meningitis using broad spectrum multiplex nested pcr]. | to propose and verify a pcr assay for detecting escherichia coli, haemophilus influenzae, listeria monocytogenes, staphylococcus species, streptococcus pneumoniae and neisseria meningitidis serogroups b and c in a single sample of the cerebrospinal fluid of patients with purulent meningitis. | 2007 | 17929222 |
| [viable but non-culturable form of bacteria]. | viable but non-culturable (vbnc) bacteria concept has been defined in 1982 when it has been shown that there exists bacteria whose metabolic activity continue and which can have the ability to reproduce in suitable conditions although they have lost their capability to reproduce in culture. recent studies have shown that most of the human pathogens (campylobacter spp., escherichia coli, francisella tularensis, helicobacter pylori, legionella pneumophila, listeria monocytogenes, mycobacterium tub ... | 2007 | 17933263 |
| enumeration of viable listeria monocytogenes cells by real-time pcr with propidium monoazide and ethidium monoazide in the presence of dead cells. | propidium monoazide (pma) and ethidium monoazide were used for enumeration of viable listeria monocytogenes cells in the presence of dead cells. pma had no antimicrobial effect on l. monocytogenes. viable cell counts were linearly related to real-time pcr threshold cycle values for pma-treated cells from planktonic and biofilm sources over a 4-log range. | 2007 | 17933922 |
| phenotypic and transcriptomic analyses demonstrate interactions between the transcriptional regulators ctsr and sigma b in listeria monocytogenes. | listeria monocytogenes sigma(b) positively regulates the transcription of class ii stress response genes; ctsr negatively regulates class iii stress response genes. to identify interactions between these two stress response systems, we constructed l. monocytogenes deltactsr and deltactsr deltasigb strains, as well as a deltactsr strain expressing ctsr in trans under the control of an iptg (isopropyl-beta-d-thiogalactopyranoside)-inducible promoter. these strains, along with a parent and a deltas ... | 2007 | 17933929 |
| morphological changes in listeria monocytogenes subjected to sublethal alkaline stress. | scanning electron microscopy (sem) studies revealed that exposure to 4lethal alkaline stress induced statistically significant (p<0.05) changes in mean cell length, radius and volume in listeria monocytogenes and a derived sigma(b) deficient mutant. bacterial morphology was altered at ph values above 9.0, to include single filamentous or elongated chain forms. such filamentation and chain formation was observed in the parent strain and in the sigma(b) deficient strain, and in buffered and non-bu ... | 2007 | 17935812 |
| listeria monocytogenes as novel carrier system for the development of live vaccines. | listeria monocytogenes is a facultative intracellular bacterium that enters a variety of non-professional mammalian cells by triggered phagocytosis ("zipper mechanism") and replicates in the cytosol of the infected host cells. therefore, it is a promising vaccine vector for the presentation of passenger antigens to the mhc class ii and especially class i pathways. here, we review recent progress made in our laboratory on the development of novel attenuated l. monocytogenes carrier strains for th ... | 2008 | 17936682 |
| development of a tripartite vector system for live oral immunization using a gram-negative probiotic carrier. | the mucosa represents the primary target site and thus the first barrier for most microbial pathogens. nevertheless, nearly all present-day vaccines are applied by an invasive route, target the systemic immune system, and do not confer efficient mucosal protection. currently, mucosal immunity can only be achieved by the delivery of antigens via the mucosal route. therefore, multiple efforts are under way to develop mucosal vaccines and particularly live oral vaccines as these would confer consid ... | 2008 | 17936683 |
| generation and maintenance of listeria-specific cd8+ t cell responses in perforin-deficient mice chronically infected with lcmv. | disruption of the perforin gene results in primary immunodeficiency and an increased susceptibility to opportunistic pathogens. perforin-deficient (pko) mice fail to clear primary lymphocytic choriomeningitis virus (lcmv) armstrong, resulting in persistent infection and functional exhaustion of virus-specific cd8+ t cells. cd8+ t cell responses to listeria monocytogenes (lm) challenge within the first week after lcmv infection were diminished in both wt and pko mice, and correlated with enhanced ... | 2008 | 17936870 |
| identification of novel listeria monocytogenes secreted virulence factors following mutational activation of the central virulence regulator, prfa. | upon bacterial entry into the cytosol of infected mammalian host cells, the central virulence regulator prfa of listeria monocytogenes becomes activated and induces the expression of numerous factors which contribute to bacterial pathogenesis. the mechanism or signal by which prfa becomes activated during the course of infection has not yet been determined; however, several amino acid substitutions within prfa (known as prfa* mutations) that appear to lock the protein into a constitutively activ ... | 2007 | 17938228 |
| live attenuated listeria monocytogenes effectively treats hepatic colorectal cancer metastases and is strongly enhanced by depletion of regulatory t cells. | the liver represents a major and frequently sole site of metastases for many types of cancer, particularly gastrointestinal cancers. we showed previously that coadministration of an engineered hepatic-targeting listeria monocytogenes (lm) with a cancer vaccine enhanced the antitumor effect of vaccine-induced t cells selectively against hepatic metastases. here, we show that administration of multiple doses of lm, in the absence of vaccine, generates therapeutic responses against hepatic metastas ... | 2007 | 17942940 |
| protective effect of lactobacillus sakei 2a against experimental challenge with listeria monocytogenes in gnotobiotic mice. | lactobacillus sakei 2a isolated from sausage and presenting an in vitro antagonistic activity against listeria monocytogenes scott a was tested for a protective effect in mice experimentally challenged with the enterobacteria. | 2007 | 17944842 |
| mesothelin targeted cancer immunotherapy. | mesothelin is a tumour differentiation antigen that is normally present on the mesothelial cells lining the pleura, peritoneum and pericardium. it is, however, highly expressed in several human cancers including malignant mesothelioma, pancreatic, ovarian and lung adenocarcinoma. the normal biologic function of mesothelin is unknown but recent studies have shown that it binds to ca-125 and may play a role in the peritoneal spread of ovarian cancer. the limited mesothelin expression in normal tis ... | 2008 | 17945478 |
| new opto-plasmonic tweezers for manipulation and rotation of biological cells--design and fabrication. | opto-plasmonic tweezers are proposed as a new optical manipulator and rotator for biological cells. the approach utilizes polarized light to excite localized surface plasmon resonance (lspr) on an array of au nanostructure. large dielectrophoretic trapping force is expected to be induced by the highly non-uniform scattering field from the resonant oscillating dipoles. fine orientation control of the cells can be realized by tuning the polarization state of the input light. | 2006 | 17946622 |
| fate of listeria spp. on parsley leaves grown in laboratory and field cultures. | to investigate the population dynamics of listeria monocytogenes and listeria innocua on the aerial surfaces of parsley. | 2007 | 17953592 |
| listeria monocytogenes in spontaneous abortions in humans and its detection by multiplex pcr. | to assess the extent of listeria monocytogenes in causation of human spontaneous abortions by isolation methods and pcr analysis for the presence of virulence-associated genes. | 2007 | 17953598 |
| listeria monocytogenes is common in wild birds in helsinki region and genotypes are frequently similar with those found along the food chain. | to evaluate the prevalence and genetic diversity of listeria monocytogenes in wild birds and to compare the genotypes with isolates previously collected from foods and food processing environments. | 2008 | 17953682 |
| detuning cd8 t cells: down-regulation of cd8 expression, tetramer binding, and response during ctl activation. | cd8 is critical for t cell recognition of peptide/class i major histocompatability complex ligands, yet is down-regulated during activation of cd8 t cells. we report that loss of cd8 expression early during in vivo responses to vaccinia virus or listeria monocytogenes (lm) correlates with decreased t cell staining with specific class i/peptide tetramers and reduced cd8 t cell sensitivity for antigen. loss of cd8 cell surface expression occurs despite sustained mrna expression, and cd8 levels ret ... | 2007 | 17954566 |
| prevalence of and risk factors for listeria species on dairy farms. | this cross-sectional study determined the prevalence of listeria spp. in bulk-tank milk on dairy farms in the region of galicia in northwest spain. the aim was to identify management practices associated with the presence of listeria spp. and possible effects on milk hygienic quality. a total of 98 farms was randomly selected on the basis of an expected prevalence of 6.5% for listeria monocytogenes from 20,107 dairy farms in the region. bulk-tank milk samples were obtained from 98 farms, fecal s ... | 2007 | 17954748 |
| effect of temperature and growth media on the attachment of listeria monocytogenes to stainless steel. | listeria monocytogenes atcc 19111 cultivated in nutrient-rich medium (brain heart infusion, bhi) or starved in minimal medium (10% filter sterilized pond water and 90% sterilized distilled water) were investigated for their initial attachment to austenitic stainless steel no. 4 with satin finish at 4 degrees c, 20 degrees c, 30 degrees c, 37 degrees c, or 42 degrees c. a droplet (10 microl) containing approximately 10(7) cfu/ml of l. monocytogenes suspended in bhi or minimal medium was placed on ... | 2007 | 17959265 |
| antibacterial effect of five zingiberaceae essential oils. | essential oil obtained by hydrodistillation and two different solvent extractions (petroleum ether and ethanol) from five zingiberaceae species: ginger (zingiber officinale roscoe.), galanga (alpinia galanga sw.), turmeric (curcuma longa l.), kaempferia (boesenbergia pandurata holtt.) and bastard cardamom (amomum xanthioides wall.) was characterized. volatile components of all extracts were analyzed by gas chromatography-mass spectrometry (gc-ms). the major components of ginger, turmeric, galang ... | 2007 | 17960105 |
| unique functions of splenic cd8alpha+ dendritic cells during infection with intracellular pathogens. | deciphering the prerequisites for the induction of protective cytotoxic t cell responses is essential for future development of more effective cd8(+) t cell-based vaccines against infectious diseases and cancer. since crucial events for cd8(+) t cell priming and differentiation occur during the first contacts of naïve t cells with distinct antigen-presenting cells (apcs), the identification and therapeutic targeting of these 'master' apcs has become a major quest in the field. a decade ago, dend ... | 2007 | 17964665 |
| the metalloprotease of listeria monocytogenes is activated by intramolecular autocatalysis. | the metalloprotease (mpl) of listeria monocytogenes is a thermolysin-like protease that mediates the maturation of a broad-range phospholipase c, whose function contributes to the ability of this food-borne bacterial pathogen to survive intracellularly. mpl is made as a proprotein that undergoes maturation by proteolytic cleavage of a large n-terminal prodomain. in this study, we identified the n terminus of mature mpl and generated mpl catalytic mutants to investigate the mechanism of mpl matur ... | 2008 | 17965168 |
| transcriptomic and phenotypic analyses suggest a network between the transcriptional regulators hrca and sigmab in listeria monocytogenes. | listeria monocytogenes hrca and ctsr negatively regulate class i and iii stress response genes, respectively, while sigma(b) positively regulates the transcription of class ii stress response genes. to define the hrca regulon and identify interactions between hrca, ctsr, and sigma(b), we characterized newly generated l. monocytogenes deltahrca, deltactsr deltahrca, and deltahrca deltasigb strains, along with previously described deltasigb, deltactsr, and deltactsr deltasigb strains, using phenot ... | 2007 | 17965207 |
| atmospheric plasma inactivation of foodborne pathogens on fresh produce surfaces. | a study was conducted to determine the effect of one atmosphere uniform glow discharge plasma (oaugdp) on inactivation of escherichia coli o157:h7, salmonella, and listeria monocytogenes on apples, cantaloupe, and lettuce, respectively. a five-strain mixture of cultured test organisms was washed, suspended in phosphate buffer, and spot inoculated onto produce (7 log cfu per sample). samples were exposed inside a chamber affixed to the oaugdp blower unit operated at a power of 9 kv and frequency ... | 2007 | 17969610 |
| effect of potassium lactate and a potassium lactate-sodium diacetate blend on listeria monocytogenes growth in modified atmosphere packaged sliced ham. | two commercially available organic acid salts, potassium lactate (purasal hipure p) and a potassium lactate-sodium diacetate blend (purasal opti. form pd 4), were assessed as potential inhibitors of listeria monocytogenes growth in modified atmosphere packaged (map) sliced ham in challenge studies. the influence of the initial inoculation level of l. monocytogenes (10(1) or 10(3) cfu g(-1)) and storage temperature (4 or 8 degrees c) was also examined. the addition of either organic acid salt to ... | 2007 | 17969611 |
| controlling listeria monocytogenes on sliced ham and turkey products using benzoate, propionate, and sorbate. | the objective of this study was to identify concentrations of sorbate, benzoate, and propionate that prevent the growth of listeria monocytogenes on sliced, cooked, uncured turkey breast and cured ham. sixteen test formulations plus a control formulation for each product type were manufactured to include potassium sorbate, sodium benzoate, or sodium propionate, used alone and combined (up to 0.3% [wt/wt]), or with sodium lactate-sodium diacetate combinations. products were inoculated with l. mon ... | 2007 | 17969612 |
| effect of packaging and storage temperature on the survival of listeria monocytogenes inoculated postprocessing on sliced salami. | the survival of postprocess listeria monocytogenes contamination on sliced salami, stored under the temperatures associated with retail and domestic storage, was investigated. sliced salami was inoculated with low and high concentrations of l. monocytogenes before being packaged under vacuum or air. survival of l. monocytogenes was determined after storage of sausages for 45 or 90 days for low or high sample inocula, respectively, at 5, 15, and 25 degrees c. all survival curves of l. monocytogen ... | 2007 | 17969613 |
| effect of salt, smoke compound, and storage temperature on the growth of listeria monocytogenes in simulated smoked salmon. | smoked salmon can be contaminated with listeria monocytogenes. it is important to identify the factors that are capable of controlling the growth of l. monocytogenes in smoked salmon so that control measures can be developed. the objective of this study was to determine the effect of salt, a smoke compound, storage temperature, and their interactions on l. monocytogenes in simulated smoked salmon. a six-strain mixture of l. monocytogenes (10(2) to 10(3) cfu/g) was inoculated into minced, cooked ... | 2007 | 17969614 |
| modeling the effect of storage atmosphere on growth-no growth interface of listeria monocytogenes as a function of temperature, sodium lactate, sodium diacetate, and nacl. | the effect of aerobic and anaerobic conditions on growth initiation by a 10-strain composite of listeria monocytogenes (10(4) cfu/ml) was evaluated in tryptic soy broth with 0.6% yeast extract (tsbye) as a function of 220 combinations of ph (3.82 to 7.42), sodium lactate (sl) (0 to 10%, vol/vol), and sodium diacetate (sd) (0 to 0.5%, wt/vol) at 10 or 30 degrees c (a slightly abusive and the optimal growth temperature, both above the growth limiting range of 0 to 3 degrees c for l. monocytogenes) ... | 2007 | 17969615 |
| antimicrobial susceptibility of listeria monocytogenes isolated from food and clinical cases in navarra, spain. | the susceptibility of 440 listeria monocytogenes strains isolated from food (n=401) and clinical cases (n=39) between 1995 and 2005 was determined by standard agar dilution and e-test methods. antimicrobial drugs currently used in veterinary and human therapy were tested, and they included penicillin g, ampicillin, cephalothin, gentamicin, chloramphenicol, tetracycline, doxycycline, trimethoprim, erythromycin, and clindamycin. the sensitivity of strains was established using clinical and laborat ... | 2007 | 17969626 |
| survey of temperature and consumption patterns of fresh-cut leafy green salads: risk factors for listeriosis. | increasing demand for fresh-cut or ready-to-eat fruits and vegetables, developed to meet the consumer need for quick and convenient products, has prompted extensive research into their microbiological quality, safety, processing, and packaging. the microbial ecology of listeria monocytogenes is recognized as a major safety concern for fresh-cut produce. a survey was performed to collect information on consumption patterns of fresh-cut leafy green salads and the temperature of domestic refrigerat ... | 2007 | 17969627 |
| blockade of endogenous b7-h1 suppresses antibacterial protection after primary listeria monocytogenes infection. | b7-h1 (also known as cd274 and pd-l1) is a cosignalling molecule regulating t-cell immunity positively or negatively in vivo. however, little is known about the role of endogenous b7-h1 in bacterial infection. we found that b7-h1 expression was up-regulated in various cell populations including cd4+ and cd8+ t cells, natural killer (nk) cells and macrophages following listeria monocytogenes infection. administration of the antagonistic b7-h1 monoclonal antibody resulted in a significant increase ... | 2008 | 17971153 |
| miniature protein ligands for evh1 domains: interplay between affinity, specificity, and cell motility. | dynamic rearrangements of the actin cytoskeleton power cell motility in contexts ranging from intracellular microbial pathogenesis to axon guidance. the ena/vasp family proteins-mena, vasp, and evl-are believed to control cell motility by serving as a direct link between signaling events and the actin cytoskeleton. it has previously been reported that a novel miniature protein, pgolemi, binds with high affinity to the evh1 domain of mena (mena1-112) but not to those of vasp (vasp1-115) or evl (e ... | 2007 | 17973491 |
| induction of suicidal erythrocyte death by listeriolysin from listeria monocytogenes. | listeriolysin, the secreted cytolysin of the facultative intracellular bacterium listeria monocytogenes, is its major virulence factor. previously, non-lytic concentrations of listeriolysin were shown to induce ca2+-permeable nonselective cation channels in human embryonic kidney cells. in erythrocytes, ca2+ entry is followed by activation of k+ channels resulting in k+-exit as well as by membrane scrambling resulting in phosphatidylserine exposure at the cell surface. phosphatidylserine-exposin ... | 2007 | 17975307 |
| isolation of the bacillus subtilis antimicrobial peptide subtilosin from the dairy product-derived bacillus amyloliquefaciens. | to purify and characterize an antimicrobial protein (bacteriocin) isolated from the dairy product-derived bacillus amyloliquefaciens. | 2008 | 17976171 |
| glycine betaine improves listeria monocytogenes tolerance to desiccation on parsley leaves independent of the osmolyte transporters betl, gbu and opuc. | to investigate the effect of glycine betaine (gb) on the survival of listeria monocytogenes on leaf surfaces under low relative humidity (rh). | 2008 | 17976173 |
| progression of pulmonary tuberculosis and efficiency of bacillus calmette-guérin vaccination are genetically controlled via a common sst1-mediated mechanism of innate immunity. | using a mouse model for genetic analysis of host resistance to virulent mycobacterium tuberculosis, we have identified a genetic locus sst1 on mouse chromosome 1, which controls progression of pulmonary tuberculosis. in vitro, this locus had an effect on macrophage-mediated control of two intracellular bacterial pathogens, m. tuberculosis and listeria monocytogenes. in this report, we investigated a specific function of the sst1 locus in antituberculosis immunity in vivo, especially its role in ... | 2007 | 17982083 |
| the aryl hydrocarbon receptor is required for optimal resistance to listeria monocytogenes infection in mice. | the aryl hydrocarbon receptor (ahr) is part of a powerful signaling system that is triggered by xenobiotic agents such as polychlorinated hydrocarbons and polycyclic aromatic hydrocarbons. although activation of the ahr by 2,3,7,8-tetrachlorodibenzo-p-dioxin or certain polycyclic aromatic hydrocarbons can lead to immunosuppression, there is also increasing evidence that the ahr regulates certain normal developmental processes. in this study, we asked whether the ahr plays a role in host resistan ... | 2007 | 17982086 |
| evidence of lethal and sublethal injury in food-borne bacterial pathogens exposed to high-intensity pulsed-plasma gas discharges. | to apply scanning electron microscopy, image analysis and a fluorescent viability stain to assess lethal and sublethal injury in food-borne bacteria exposed to pulsed-plasma gas discharges (ppgd). | 2008 | 17983430 |
| an itam-signaling pathway controls cross-presentation of particulate but not soluble antigens in dendritic cells. | dendritic cells (dc) possess a unique capacity for presenting exogenous antigen on major histocompatibility class i, a process that is referred to as cross-presentation, which serves a critical role in microbial and tumor immunity. during cross-presentation, antigens derived from pathogen-infected or tumor cells are internalized and processed by dcs for presentation to cytotoxic t lymphocytes (ctls). we demonstrate that a signaling pathway initiated by the immunoreceptor tyrosine-based activatio ... | 2007 | 17984307 |
| isolation of listeria monocytogenes from the skin of slaughtered beef cattle. | we attempted to isolate listeria monocytogenes from skin, contents of large intestines and carcasses of cattle introduced to a slaughterhouse in order to identify source of contamination for this pathogen. sixty skin samples, 60 samples of the contents of large intestines and 30 carcass samples were colleted in june, august and november 2003 for use in this study. listeria spp. and l. monocytogenes were isolated from 30 (50%) and 3 (5%) of the cattle skin samples, respectively. however, no liste ... | 2007 | 17984598 |
| the development of a 'labeless' immunosensor for the detection of listeria monocytogenes cell surface protein, internalin b. | electrochemical impedance spectroscopy (eis) is a widely used technique for probing bioaffinity interactions at the surfaces of electrically conducting polymers. eis methods can be employed to investigate 'labeless' detection of analytes via impedimetric transduction. this paper describes the development of a direct immunosensor for the detection of a cell-surface protein on listeria monocytogenes, an extremely important food-borne pathogen. l. monocytogenes are facultative anaerobic, non-sporin ... | 2008 | 17988853 |
| listeriosis in a cockatiel (nymphicus hollandicus). | listeriosis was diagnosed in a 4-yr-old female cockatiel (nymphicus hollandicus) that died after exhibiting clinical signs that included a fluffed-up appearance, weakness, and loss of weight of several days duration. grossly, the bird was moderately emaciated, and the liver and spleen were enlarged. microscopically, there was mild-to-moderate inflammation associated with rod-shaped, gram-positive bacteria in the liver, spleen, kidneys, adrenal glands, bone marrow, and esophagus. listeria monocyt ... | 2007 | 17992946 |
| reduction of bacteria on spinach, lettuce, and surfaces in food service areas using neutral electrolyzed oxidizing water. | food safety issues and increases in food borne illnesses have promulgated the development of new sanitation methods to eliminate pathogenic organisms on foods and surfaces in food service areas. electrolyzed oxidizing water (eo water) shows promise as an environmentally friendly broad spectrum microbial decontamination agent. eo water is generated by the passage of a dilute salt solution ( approximately 1% nacl) through an electrochemical cell. this electrolytic process converts chloride ions an ... | 2008 | 17993375 |
| rapid real-time pcr detection of listeria monocytogenes in enriched food samples based on the ssra gene, a novel diagnostic target. | a real-time pcr assay was designed to detect a 162-bp fragment of the ssra gene in listeria monocytogenes. the specificity of the assay for l. monocytogenes was confirmed against a panel of 6 listeria species and 26 other bacterial species. a detection limit of 1-10 genome equivalents was determined for the assay. application of the assay in natural and artificially contaminated culture enriched foods, including soft cheese, meat, milk, vegetables and fish, enabled detection of 1-5 cfu l. monocy ... | 2008 | 17993379 |
| the antimicrobial effect of thyme essential oil, nisin, and their combination against listeria monocytogenes in minced beef during refrigerated storage. | the antimicrobial effect of thyme essential oil (eo) at 0.3%, 0.6%, or 0.9%, nisin at 500 or 1000iu/g, and their combination against listeria monocytogenes was examined in both tryptic soy broth (tsb) and minced beef meat. thyme eo at 0.3% possessed a weak antibacterial activity against the pathogen in tsb, whereas at 0.9% showed unacceptable organoleptic properties in minced meat. thus, only the level of 0.6% of eo was further examined against the pathogen in minced meat. treatment of minced be ... | 2008 | 17993385 |
| high-pressure processing and antimicrobial biodegradable packaging to control listeria monocytogenes during storage of cooked ham. | the efficiency of combining high-pressure processing (hpp) and active packaging technologies to control listeria monocytogenes growth during the shelf life of artificially inoculated cooked ham was assessed. three lots of cooked ham were prepared: control, packaging with alginate films, and packaging with antimicrobial alginate films containing enterocins. after packaging, half of the samples were pressurized. sliced cooked ham stored at 6 degrees c experienced a quick growth of l. monocytogenes ... | 2008 | 17993392 |
| subtractive screening for probiotic properties of lactobacillus species from the human gastrointestinal tract in the search for new probiotics. | in the search for new probiotics, 61 lactobacillus spp. isolates, belonging to 12 species and isolated as dominant lactic acid bacteria from the feces of healthy humans, were subjected to a subtractive system of in vitro analyses, which included desirable and undesirable traits. twenty-four isolates were able to grow in 2% bovine bile, of which 13 grew in acidified broth at ph 3.5 in acidified cysteine-containing mrs broth. intrinsic resistance to certain antimicrobial agents (cefoxitin, metroni ... | 2007 | 17995611 |
| antimicrobial activity of epsilon-polylysine against escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in various food extracts. | this study compared the antimicrobial effects of epsilon-polylysine (epsilon-pl) against escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in 6 food extracts and in broth. the food extracts (10% (w/w) in distilled water) evaluated were fat-free and whole fat milk, beef, bologna, rice, and vegetables (50:50 ratio of broccoli and cauliflower). epsilon-pl was tested at 0.005% and 0.02% (w/v) against e. coli o157:h7 and l. monocytogenes, and 0.02% and 0.04% (w/v) against s ... | 2007 | 17995614 |
| modeling the lag phase and growth rate of listeria monocytogenes in ground ham containing sodium lactate and sodium diacetate at various storage temperatures. | refrigerated ready-to-eat (rte) meats contaminated with listeria monocytogenes were implicated in several listeriosis outbreaks. lactate and diacetate have been shown to control l. monocytogenes in rte meats. the objective of this study was to examine and model the effect of lactate (1.0% to 4.2%) and diacetate (0.05% to 0.2%) in ground ham on the lag phase duration (lpd, h) and growth rate (gr, log cfu/h) of l. monocytogenes at a range of temperatures (0 to 45 degrees c). a 6-strain mixture of ... | 2007 | 17995648 |
| impact of nitrite on detection of listeria monocytogenes in selected ready-to-eat (rte) meat and seafood products. | the impact of sodium nitrite (nano2) on detection and recovery of listeria monocytogenes from select ready-to-eat (rte) foods including smoked salmon, smoked ham, beef frankfurters, and beef bologna was assessed. nitrite-containing (nc; 100 to 200 ppm nano2) or nitrite-free (nf) foods were inoculated with a 5-strain cocktail of l. monocytogenes by immersion into butterfield's buffer solution containing 5.4 to 7.4 x 10(3) l. monocytogenes per milliliter. inoculated products were vacuum-packaged a ... | 2007 | 17995651 |
| characterization and selection of autochthonous lactic acid bacteria isolated from traditional iberian dry-fermented salchichón and chorizo sausages. | a total of 192 lactic acid bacteria were isolated from 2 types of naturally fermented dry sausages at 3 different stages of the ripening process in order to select the most suitable strains as starter cultures in dry-cured sausage manufacture according to their technological characteristics such as glucose fermentation, lactic and acetic acid production, and proteolytic, lipolytic, and antimicrobial activities. identification of the isolates revealed that 31.2% were pediococcus pentosaceus, 26.9 ... | 2007 | 17995686 |
| recipes for antimicrobial wine marinades against bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, and salmonella enterica. | we have evaluated bactericidal activities against bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, and salmonella enterica of several antimicrobial wine recipes, each consisting of red or white wine extracts of oregano leaves with added garlic juice and oregano oil. dose-response plots were used to determine the percentage of the recipes that resulted in a 50% decrease in colony-forming units (cfu) at 60 min (ba(50)). studies designed to optimize antibacterial activities of the ... | 2007 | 17995688 |
| effect of ph on the antimicrobial activity and oxidative stability of oil-in-water emulsions containing caffeic acid. | antioxidant properties in food are dependent on various parameters. these include the ph value and interactions with food components, including proteins or metal ions. food components affect antioxidant stability and also influence the properties of microorganisms and their viability. this paper describes an investigation of the effect of ph on the antioxidant and antibacterial properties of caffeic acid in different media. the ph values studied, using an oil-in-water emulsion as model system, w ... | 2007 | 17995712 |
| numerical analysis of survival of listeria monocytogenes during in-package pasteurization of frankfurters by hot water immersion. | the objective of this research was to develop and validate a more accurate method to analyze and calculate the inactivation of listeria monocytogenes in frankfurter packages during postlethality hot water immersion heating and the subsequent cooling processes. finite difference analysis with implicit scheme was used to simulate the heat transfer process during in-package pasteurization of frankfurters. a volumetrically distributed simulation method was developed to calculate the lethality of the ... | 2007 | 17995728 |
| effect of water phase salt content and storage temperature on listeria monocytogenes survival in chum salmon (oncorhynchus keta) roe and caviar (ikura). | salmon caviar, or ikura, is a ready-to-eat food prepared by curing the salmon roe in a brine solution. other seasonings or flavorants may be added, depending upon the characteristics of the product desired. listeria monocytogenes growth is a potential risk, since it can grow at high salt concentrations (>10%) and in some products at temperatures as low as 3 degrees c. ikura was prepared from chum salmon (oncorhynchus keta) roe by adding food-grade nacl to yield water phase salt contents (wps) of ... | 2007 | 17995738 |
| uncertainty in thermal process calculations due to variability in first-order and weibull kinetic parameters. | alternatives to first-order model of death kinetics of microorganisms have been proposed as improvements in the calculation of lethality for a thermal process. although such models can potentially improve predictions for many situations, this article tries to answer the question of whether the added complexities of these models are a worthwhile investment once we include the effect of uncertainties in various microbiological and process parameters. monte carlo technique is used to include variab ... | 2007 | 17995767 |
| inactivation of shigella boydii 18 idph and listeria monocytogenes scott a with power ultrasound at different acoustic energy densities and temperatures. | the effect of acoustic energy density (aed) on inactivation of shigella boydii 18 idph and listeria monocytogenes scott a in a cell suspension was studied at sublethal temperatures and at aeds of 0.49, 0.85, and 1.43 w/ml. the effect of temperature on ultrasonic inactivation of l. monocytogenes scott a at 35, 50, and 65 degrees c was examined at an aed of 1.43 w/ml. increasing aed increased the rate of inactivation for both s. boydii and l. monocytogenes. the destruction of s. boydii and l. mono ... | 2007 | 17995776 |
| a 2-step cooking method of searing and hot water pasteurization to maximize the safety of refrigerated, vacuum packaged, chicken breast meat. | americans consume almost 40 kg per capita of chicken each year. increasing consumption of chicken surpassed pork in 1982 and beef in 1992. the objectives of this study were to examine the effectiveness of a novel, 2-step cooking method of grilling, slicing, vacuum packaging, and hot water pasteurization to inhibit the growth of listeria monocytogenes in chicken breast meat. because this study required the use of pilot plant scale pasteurization equipment, listeria innocua m1, a nonpathogen with ... | 2007 | 17995778 |
| inhibition of foodborne pathogens by native microflora recovered from fresh peeled baby carrot and propagated in cultures. | the inhibitory effect of fresh peeled baby carrot and associated microflora on the viability and growth of listeria monocytogenes, yersinia enterocolitica, salmonella enterica, escherichia coli o157:h7, and pseudomonas marginalis was investigated. the population of viable l. monocytogenes was reduced by more than 2 logs following the dipping of sliced baby carrots in the bacterial suspension for 2 min. however, other pathogens subjected to the same treatment showed no reduction in the population ... | 2007 | 17995781 |
| thermal inactivation of escherichia coli o157:h7, salmonella, and listeria monocytogenes in breaded pork patties. | decimal reduction times (d-values) and thermal resistance constants (z-values) for 3 foodborne pathogenic bacteria in formulated ready-to-eat breaded pork patties were determined with thermal inactivation studies. meat samples, inoculated with escherichia coli o157:h7, salmonella, and listeria monocytogenes cultures or uninoculated controls, were packaged in sterile bags, immersed in circulated water bath, and held at 55, 57.5, 60, 62.5, 65, 67.5, and 70 degrees c for different durations of time ... | 2007 | 17995843 |
| invasiveness and intracellular growth of multidrug-resistant salmonella and other pathogens in caco-2 cells. | the increase of multidrug-resistant pathogens of human and animal origins is a major public health concern. for a better understanding of the health consequences of multidrug-resistant bacteria transmitted from animal products to humans, the host interaction of zoonotic salmonella isolates along with other pathogenic and commensal bacteria was evaluated using a human intestinal caco-2 cell system. multidrug-resistant s. agona, s. heidelberg, and s. typhimurium possessed plasmid-mediated class 1 ... | 2007 | 17995846 |
| characterization of new hybridoma clones producing monoclonal antibodies reactive against both live and heat-killed listeria monocytogenes. | the objective of this study is to develop high affinity monoclonal antibody (mab) probes recognizing all major serotypes of listeria monocytogenes cells. from 500 candidate hybridoma clones, 2 new monoclonal antibody-producing hybridomas were selected and evaluated. mabs 22d10 and 24f6 reacted strongly with live cells of most serotypes of l. monocytogenes except 4c and 4e and with some l. innocua strains; mab 22d10 reacted strongly with both live and heat-killed cells (100 degrees c for 20 min) ... | 2007 | 17995886 |
| searching for new mathematical growth model approaches for listeria monocytogenes. | different secondary modeling approaches for the estimation of listeria monocytogenes growth rate as a function of temperature (4 to 30 degrees c), citric acid (0% to 0.4% w/v), and ascorbic acid (0% to 0.4% w/v) are presented. response surface (rs) and square-root (sr) models are proposed together with different artificial neural networks (ann) based on product functions units (pu), sigmoidal functions units (su), and a novel approach based on the use of hybrid functions units (psu), which resul ... | 2007 | 17995887 |
| effectiveness of potassium lactate and sodium diacetate in combination with irradiation to control listeria monocytogenes on frankfurters. | the use of antimicrobial ingredients in combination with irradiation is an effective antilisterial intervention strategy for ready-to-eat meat products. microbial safety was evaluated for frankfurters formulated with 0% or 3% added potassium lactate/sodium diacetate solution and inoculated with listeria monocytogenes before or after treatment with irradiation (0, 1.8, or 2.6 kgy). frankfurters were stored aerobically or vacuum packaged and l. monocytogenes counts and apcs were determined while r ... | 2007 | 17995888 |
| epigallocatechin gallate inhibits intracellular survival of listeria monocytogenes in macrophages. | epigallocatechin gallate (egcg), the major tea catechin, is known as a potent anti-microbial and anti-tumor compound. the effects of egcg on host defense mechanisms against listeria monocytogenes infection were examined in vitro using mouse peritoneal exudate cells. the study showed that egcg inhibited the intracellular growth of l. monocytogenes in macrophages. the enhancement of in vitro anti-l. monocytogenes activity by egcg is not due to the modulation of reactive oxygen intermediates or the ... | 2008 | 17996193 |
| community-acquired bacterial meningitis in cirrhotic patients. | the hospital universitari de bellvitge (barcelona, spain) records all cases of bacterial meningitis in a 120-variable database. the characteristics of bacterial meningitis in cirrhotic patients are not well-known, and all cases of community-acquired bacterial meningitis occurring in cirrhotic patients were therefore identified. during 1977-2002, there were 602 episodes of community-acquired bacterial meningitis in adults, of which 29 (4.8%) occurred in cirrhotic patients. compared to non-cirrhot ... | 2008 | 18005179 |
| controlling listeria monocytogenes in cottage cheese through heterologous production of enterocin a by lactococcus lactis. | enterocin a is an example of a class iia bacteriocin with potent anti-listerial activity. this study was initiated with a view to harnessing this activity, through heterologous production by a lactococcal starter strain, to limit levels of listeria monocytogenes in a food (cottage cheese). | 2008 | 18005345 |
| results of a 12-month long enhanced surveillance of listeriosis in italy. | since 1993, the reporting of listeriosis has been mandatory in italy. the surveillance system based on case notifications from physicians is managed by the ministry of health. the information collected includes only gender, age and case distribution by region. to gather more information, an active surveillance was conducted for 12 months (2002-2003). all hospital microbiological laboratories in italy (n=103) were given clinical and food questionnaires and were requested to report positive cases ... | 2007 | 18005656 |
| a staphylococcus aureus regulatory system that responds to host heme and modulates virulence. | staphylococcus aureus, a bacterium responsible for tremendous morbidity and mortality, exists as a harmless commensal in approximately 25% of humans. identifying the molecular machinery activated upon infection is central to understanding staphylococcal pathogenesis. we describe the heme sensor system (hssrs) that responds to heme exposure and activates expression of the heme-regulated transporter (hrtab). inactivation of the hss or hrt systems leads to increased virulence in a vertebrate infect ... | 2007 | 18005689 |
| designer bugs: structural engineering to build a better mouse model. | many animal models of bacterial diseases are hampered by differences in tissue tropism and the course of pathogenesis. in a recent issue of cell, by rationally mutating a surface invasion protein (inla) to have higher binding affinity for its cognate host receptor (e-cadherin), wollert et al. were able to "murinize"listeria monocytogenes, creating a strain capable of invading intestinal epithelial cells in mice, mimicking the route of infection in humans. | 2007 | 18005702 |
| invasive and adherent bacterial pathogens co-opt host clathrin for infection. | infection by the bacterium listeria monocytogenes depends on host cell clathrin. to determine whether this requirement is widespread, we analyzed infection models using diverse bacteria. we demonstrated that bacteria that enter cells following binding to cellular receptors (termed "zippering" bacteria) invade in a clathrin-dependent manner. in contrast, bacteria that inject effector proteins into host cells in order to gain entry (termed "triggering" bacteria) invade in a clathrin-independent ma ... | 2007 | 18005755 |
| spontaneous splenic rupture associated with listeria endocarditis. | | 2007 | 18022517 |
| reversible nk1.1 surface expression on invariant liver natural killer t cells during listeria monocytogenes infection. | the invariant (i) natural killer (nk)t cells consistently express the valpha14 chain of the t cell receptor (tcr) and recognize alpha-galactosylceramide (alpha-galcer) presented by the nonpolymorphic presentation molecule cd1d. despite their name, the inkt cells represent a heterogeneous population, which can be divided on the basis of nk1.1 surface expression. here we show that nk1.1 surface expression on liver inkt cells in mice fluctuates during listeria monocytogenes infection. at early stag ... | 2007 | 18024119 |
| the role of apoptosis in listeria monocytogenes neural infection: listeriolysin o interaction with neuroblastoma neuro-2a cells. | listeria monocytogenes is the etiological agent of meningitis that affects individuals at high risk such as pregnant women, neonates, the elderly and immunocompromised individuals. infection by this intracellular pathogen can be lethal if not diagnosed and treated. mouse neuroblastoma neuro-2a cells, a neuron-like cell line, were infected with l. monocytogenes. in this study apoptotic changes of neuroblastoma neuro-2a cells infected with strains of listeria producing different listeriolysin o le ... | 2008 | 18024235 |
| comparative analysis of the sigma b-dependent stress responses in listeria monocytogenes and listeria innocua strains exposed to selected stress conditions. | the alternative sigma factor sigma(b) contributes to transcription of stress response and virulence genes in diverse gram-positive bacterial species. the composition and functions of the listeria monocytogenes and listeria innocua sigma(b) regulons were hypothesized to differ due to virulence differences between these closely related species. transcript levels in stationary-phase cells and in cells exposed to salt stress were characterized by microarray analyses for both species. in l. monocytog ... | 2008 | 18024685 |
| defective t cell development and function in the absence of abelson kinases. | thymocyte proliferation, survival, and differentiation are tightly controlled by signaling from the pre-tcr. in this study, we show for the first time that the abelson (abl) kinases regulate proximal signaling downstream of the pre-tcr. conditional deletion of abl kinases in thymocytes reveals a cell-autonomous role for these proteins in t cell development. the conditional knockout mice have reduced numbers of thymocytes, exhibit an increase in the percentage of the cd4(-)cd8(-) double-negative ... | 2007 | 18025176 |
| activation of immature hepatic nk cells as immunotherapy for liver metastatic disease. | nk cells can identify and eliminate emerging tumors due to altered expression of activating and inhibitory ligands on aberrant cells, a process that is greatly enhanced following nk cell activation. as a principal site of both tumor metastases and immature nk cells, the liver represents a unique anatomic location in which activation of the innate immune system could provide substantial therapeutic benefit. we describe here the nk cell-dependent destruction of a primary hepatic tumor following in ... | 2007 | 18025181 |
| extensive characterization of ifn-induced gtpases mgbp1 to mgbp10 involved in host defense. | ifn-gamma orchestrates a potent antimicrobial host response. however, the underlying molecular basis for this immunological defense system is largely unknown. in a systematic approach to identify ifn-gamma-regulated host effector molecules, a notable number of transcripts with consensus gtp-binding motives were obtained. further extensive transcriptome and genome analyses identified five novel family members of murine guanylate-binding proteins (mgbps) now designated mgbp6, 7, 8, 9, and 10. more ... | 2007 | 18025219 |
| production of nisin z by lactococcus lactis isolated from dahi. | lactococcus lactis cm1, an isolate from homemade "dahi," a traditional fermented milk from india, used maltose as carbon source to produce a high level of bacteriocin. the bacterial cell mass and the bacteriocin production correlated with the initial ph of the medium and were highest when the initial ph was 11.0. the level of bacteriocin reached its peak at the late log phase with concomitant reduction of culture ph to 4.2, regardless of the initial ph of the medium. a combination of maltose and ... | 2007 | 18025595 |
| characteristics and frequency of detection of fecal listeria monocytogenes shed by livestock, wildlife, and humans. | listeria monocytogenes is a facultative intracellular pathogen that can be carried asymptomatically in various animals and can be shed in feces. we investigated the prevalence and characteristics of l. monocytogenes isolated from livestock, wildlife, and human potential sources of contamination in 2 areas in ontario, canada. from february 2003 to november 2005, a total of 268 fecal samples were collected from different animals. listeria monocytogenes was isolated using selective enrichment, isol ... | 2007 | 18026208 |
| neuroinfections due to listeria monocytogenes. | listeria monocytogenes is not a rare pathogen causing meningitis, mainly in small children and in close contacts to livestock. the pathogen is naturally resistant to cephalosporins and some glycopeptides as well, therefore despite of syndromologic diagnosis of meningitis and initial therapy with 3rd generation cephalosporins according to the guidelines therapeutic failures with clinical consequences may occur. | 2007 | 18030269 |
| rapid exonuclease digestion of pcr-amplified targets for improved microarray hybridization. | | 2007 | 18030700 |
| comparison of 3m petrifilm environmental listeria plates against standard enrichment methods for the detection of listeria monocytogenes of epidemiological significance from environmental surfaces. | environmental monitoring using sensitive methods for detection and elimination of harborage sites of listeria monocytogenes is key to the control of this organism. the 3m petrifilm environmental listeria (el) plate-a no enrichment method-was compared with the usda/fsis, modified usda/fsis (musda), and iso methods for detection/recovery of l. monocytogenes on 4 environmental surfaces (brick, dairy board, stainless steel, and epoxy resin). the efficacy of 3 sampling devices including the microbial ... | 2007 | 18034727 |
| antimicrobial chitosan-lysozyme (cl) films and coatings for enhancing microbial safety of mozzarella cheese. | this study investigated the antimicrobial activities of chitosan-lysozyme (cl) composite films and coatings against tested microorganisms inoculated onto the surface of mozzarella cheese. cl film-forming solutions (ffs) with a ph of 4.4 to 4.5 were prepared by incorporating 0% or 60% lysozyme (per dry weight of chitosan) into chitosan ffs with or without a ph adjustment to 5.2. sliced cheese was subjected to 3 cl package applications: film, lamination on a multilayer coextruded film, and coating ... | 2007 | 18034728 |
| antimicrobial effect of electrolyzed oxidizing water against escherichia coli o157:h7 and listeria monocytogenes on fresh strawberries (fragaria x ananassa). | antibacterial activity of electrolyzed oxidizing (eo) water prepared from 0.05% or 0.10% (w/v) sodium chloride (nacl) solutions against indigenous bacteria associated with fresh strawberries (fragaria x ananassa) was evaluated. the efficacy of eo water and sodium hypochlorite (naocl) solution in eliminating and controlling the growth of listeria monocytogenes and escherichia coli o157:h7 inoculated onto strawberries stored at 4 +/- 1 degrees c up to 15 d was investigated at exposure time of 1, 5 ... | 2007 | 18034734 |