| effect of plant age on endophytic bacterial diversity of balloon flower (platycodon grandiflorum) root and their antimicrobial activities. | balloon flower (platycodon grandiflorum) is widely cultivated vegetable and used as a remedy for asthma in east asia. experiments were conducted to isolate endophytic bacteria from 1-, 3-, and 6-year-old balloon flower roots and to analyze the enzymatic, antifungal, and anti-human pathogenic activities of the potential endophytic biocontrol agents obtained. total 120 bacterial colonies were isolated from the interior of all balloon flower roots samples. phylogenetic analysis based on 16s rrna ge ... | 2010 | 20221603 |
| bacillus-based biocontrol of fusarium disease on tomato cultures in burundi. | the biocontrol potential of a plant growth-promoting rhizobacterium (pgpr) bacillus subtilis s499 on tomato was studied in open field sites in low attitude area of the plain of imbo in burundi. application of the pgpr strain on seed before sowing have significantly increased growth and fruit yield of tomato plants in addition to its remarkable control of a local important disease caused by a fungus type-fusarium.. this pathogen causing large tosses in burundi tomato plantings is closely related ... | 2009 | 20222546 |
| a method to confer salinity stress tolerance to plants by helicase overexpression. | high salinity stress adversely affects plant growth and limits agricultural production worldwide. to minimize these losses it is essential to develop stress-tolerant plants. several genes, including the genes encoding for helicases, are induced in response to salinity stress. helicases are ubiquitous motor enzymes that catalyze the unwinding of energetically stable duplex dna (dna helicases) or duplex rna secondary structures (rna helicases) in an atp-dependent manner. helicase members of dead-b ... | 2010 | 20225163 |
| agrobacterium induces expression of a host f-box protein required for tumorigenicity. | agrobacterium exports dna into plant cells, eliciting neoplastic growths on many plant species. during this process, a skp1-cdc53-cullin-f-box (scf) complex that contains the bacterial virulence f-box protein virf facilitates genetic transformation by targeting for proteolysis proteins, the agrobacterium protein vire2 and the host protein vip1, that coat the transferred dna. however, some plant species do not require virf for transformation. here, we show that agrobacterium induces expression of ... | 2010 | 20227663 |
| genetic characterization of root-nodule bacteria associated with acacia salicina and a. stenophylla (mimosaceae) across south-eastern australia. | symbiotic relationships between legumes and nitrogen-fixing soil micro-organisms are of ecological importance in plant communities worldwide. for example, nutrient-poor australian soils are often dominated by shrubby legumes (e.g. species of acacia). however, relatively few studies have quantified patterns of diversity, host-specificity and effectiveness of these ecologically important plant-microbe interactions. in this study, 16s rrna gene sequence and pcr-rflp analyses were used to examine ba ... | 2011 | 20228207 |
| optimisation of the purification process of a tumour-targeting antibody produced in n. benthamiana using vacuum-agroinfiltration. | it was previously demonstrated that the tumour-targeting antibody mab h10 can be transiently expressed and purified at high levels in nicotiana benthamiana by using a vacuum-agroinfiltration system boosted by the use of a virus silencing suppressor protein. scope of this work was to analyse different steps of protein extraction from agroinfiltrated leaves to optimise the purification process of the secretory mab h10 providing new insights in the field of large-scale plant production. two differe ... | 2010 | 20229286 |
| agrobacterium-meditated gene disruption using split-marker in grosmannia clavigera, a mountain pine beetle associated pathogen. | grosmannia clavigera is a fungal pathogen associated with the mountain pine beetle (dendroctonus ponderosae) which is devastating large areas of western canada's conifer forests. this fungus also produces a dark melanin pigment that discolors pine sapwood. we have generated the draft genome of g. clavigera. however, functional characterization of genes identified in the genome sequence requires an efficient gene disruption method. in this work, we report a gene replacement strategy for g. clavig ... | 2010 | 20229292 |
| probing the catalytic roles of arg548 and gln552 in the carboxyl transferase domain of the rhizobium etli pyruvate carboxylase by site-directed mutagenesis. | the roles of arg548 and gln552 residues in the active site of the carboxyl transferase domain of rhizobium etli pyruvate carboxylase were investigated using site-directed mutagenesis. mutation of arg548 to alanine or glutamine resulted in the destabilization of the quaternary structure of the enzyme, suggesting that this residue has a structural role. mutations r548k, q552n, and q552a resulted in a loss of the ability to catalyze pyruvate carboxylation, biotin-dependent decarboxylation of oxaloa ... | 2010 | 20230056 |
| mutation in the pssm gene encoding ketal pyruvate transferase leads to disruption of rhizobium leguminosarum bv. viciae-pisum sativum symbiosis. | to study the question whether acidic exopolysaccharide (eps) modification, e.g. pyruvylation, plays any role in the development of rhizobium leguminosarum/pisum sativum symbiosis. | 2010 | 20233262 |
| functional characterization and manipulation of the apicidin biosynthetic pathway in fusarium semitectum. | apicidin is a cyclic tetrapeptide produced by certain isolates of fusarium semitectum and has been shown to inhibit apicomplexan histone deacetylase. an apicidin-producing strain (kctc16676) of the filamentous fungus was mutated using an agrobacterium tumefaciens-mediated transformation, resulting in 24 apicidin-deficient mutants. three of the mutants had a t-dna insertion in a gene that encodes a non-ribosomal peptide synthetase (nrps). results of sequence, expression, and gene deletion analyse ... | 2010 | 20233305 |
| in situ raman and x-ray spectroscopies to monitor microbial activities under high hydrostatic pressure. | until recently, monitoring of cells and cellular activities at high hydrostatic pressure (hhp) was mainly limited to ex situ observations. samples were analyzed prior to and following the depressurization step to evaluate the effect of the pressure treatment. such ex situ measurements have several drawbacks: (i) it does not allow for kinetic measurements and (ii) the depressurization step often leads to artifactual measurements. here, we describe recent advances in diamond anvil cell (dac) techn ... | 2010 | 20233376 |
| heme-responsive dna binding by the global iron regulator irr from rhizobium leguminosarum. | heme, a physiologically crucial form of iron, is a cofactor for a very wide range of proteins and enzymes. these include dna regulatory proteins in which heme is a sensor to which an analyte molecule binds, effecting a change in the dna binding affinity of the regulator. given that heme, and more generally iron, must be carefully regulated, it is surprising that there are no examples yet in bacteria in which heme itself is sensed directly by a reversibly binding dna regulatory protein. here we s ... | 2010 | 20233710 |
| phosphomannose isomerase gene for selection in lettuce (lactuca sativa l.) transformation. | a positive selection system using phosphomannose isomerase was employed for agrobacterium tumefaciens mediated transformation of lettuce (lactuca sativa l. var. 'achát'). it was shown that the mannose-based selection system works very well with the lettuce genotype used, reaching up to 25% transformation efficiency on the medium with 20 g/l mannose and 20 g/l sucrose. the best transformation efficacy with the commonly-used kanamycin at 100 mg/l as a selection agent was 21%. southern blot analyse ... | 2010 | 20234883 |
| electroporation and agrobacterium-mediated spore transformation. | genetic transformation is a key technology in modern fungal research. most commonly, protoplasts are transformed using the polyethylene glycol-mediated transformation protocols. because protoplasts are generated by treatment of mycelia with a crude enzyme preparation, the results tend to be inconsistent. furthermore, some species cannot be transformed by this method. electroporation (ep) and agrobacterium tumefaciens-mediated transformation (amt) are two alternative methods. these methods allow ... | 2010 | 20238258 |
| combined influence of temperature and urethan on the respiration of rhizobium. | | 1947 | 20240501 |
| some effects of 2,4-d, ddt, and colorado 9 on the bacteria rhizobium leguminosarum frank in the root nodules of the common bean. | | 1947 | 20252514 |
| competition of rhizobium strains in nodule-formation. | | 1947 | 20255074 |
| the biological interactions of rhizobium to its host legume. | | 1947 | 20255076 |
| hemoprotein from root nodules and nitrogen fixation by rhizobium. | | 1947 | 20270738 |
| the green fluorescent protein as an efficient selection marker for agrobacterium tumefaciens-mediated transformation in hevea brasiliensis (müll. arg). | an efficient genetic transformation procedure using a recombinant green fluorescent protein (gfp) has been developed in hevea brasiliensis clone pb260. transformation experiments have been performed using an agrobacterium tumefaciens binary vector harbouring both uida and s65t-gfp reporter genes in order to compare selection methods using glucuronidase assay (gus activity) and paromomycin resistance, gfp activity and paromomycin resistance, or gfp activity only. at transient level, the number of ... | 2010 | 20306052 |
| synergism of vam and rhizobium on production and metabolism of iaa in roots and root nodules of vigna mungo. | mature and healthy root nodules of vigna mungo appeared to contain higher amount of indole-acetic acid (iaa) than non-nodulated roots. dual effect of vam fungus, glomus fasciculatum and the nitrogen-fixing bacteria, rhizobium sp. on the nodulation of roots of v. mungo was studied. it was recorded that the roots which were inoculated simultaneously with both the symbionts i.e., g. fasciculatum and rhizobium exhibited greater amount of iaa production than the non-inoculated roots. a tryptophan poo ... | 2010 | 20306316 |
| [effects of rhizobium meliloti on pcbs degradation and transformation in solution culture]. | the capability of rhizobium meliloti on degrading 2,4,4'-trichlorobiphenyl and 18 kinds of polychlorinated biphenyl congener mixtures was studied by shaking flask experiment. the results showed that the degradation capability of rhizobium meliloti to 2, 4, 4'-trichlorobiphenyl increased gradually with the increasing concentration of 2, 4, 4'-trichlorobiphenyl. after 7 days, the degradation rates of 2, 4, 4'-trichlorobiphenyl under 1.0, 5.0 10.0, 25.0, 50.0 mg x l(-1) concentration of 2, 4, 4'-tr ... | 2010 | 20329548 |
| light-induced activation of bacterial phytochrome agp1 monitored by static and time-resolved ftir spectroscopy. | phytochromes, which regulate many biological processes in plants, bacteria, and fungi, can exist in two stable states, pr and pfr, that can be interconverted by light, via a number of intermediates such as meta-rc. herein we employ ftir spectroscopy to study the pr-to-pfr conversion of the bacteriophytochrome agp1 from agrobacterium tumefaciens. static ftir pfr/pr and meta-rc/pr difference spectra are disentangled in terms of cofactor and protein structural changes. guided by dft calculations on ... | 2010 | 20333618 |
| effect of miconazole and terbinafine on artemisinin content of shooty teratoma of artemisia annua. | the effects produced by the addition of sterol synthesis inhibitors on the artemisinin content of the transgenic organ culture (a. tumefaciens atcc 33970 or 15955) of artemisia annua are presented. the transgenic tissue produced 3-4 fold higher levels of artemisinin 0.84% (56.3 mg/l) within a short culture period compared with field grown plants (0.23%). the addition of the sterol synthesis inhibitors, miconazole and terbinafine, to these transgenic cultures resulted in enhanced artemisinin cont ... | 2010 | 20334124 |
| plant proteins involved in agrobacterium-mediated genetic transformation. | agrobacterium species genetically transform plants by transferring a region of plasmid dna, t-dna, into host plant cells. the bacteria also transfer several virulence effector proteins. t-dna and virulence proteins presumably form t-complexes within the plant cell. super-t-complexes likely also form by interaction of plant-encoded proteins with t-complexes. these protein-nucleic acid complexes traffic through the plant cytoplasm, enter the nucleus, and eventually deliver t-dna to plant chromatin ... | 2010 | 20337518 |
| use of elisa with antiexopolysaccharide antibodies to evaluate wheat-root colonization by the rhizobacterium paenibacillus polymyxa. | enzyme-linked immunosorbent assay with rabbit polyclonal antibodies developed to isolated exopolysaccharide of paenibacillus polymyxa 1465 was used to evaluate the colonization of wheat-seedling roots by this bacterium. the assay conditions were optimized for detection of the p. polymyxa exopolysaccharide determinants forming part of the samples used (homogenates of inoculated roots). the dynamics of the immunoenzymatic revealing of specific polysaccharidic antigenic determinants in the samples' ... | 2010 | 20339848 |
| agrobacterium tumefaciens type iv secretion protein virb3 is an inner membrane protein and requires virb4, virb7, and virb8 for stabilization. | agrobacterium tumefaciens virb proteins assemble a type iv secretion apparatus and a t-pilus for secretion of dna and proteins into plant cells. the pilin-like protein virb3, a membrane protein of unknown topology, is required for the assembly of the t-pilus and for t-dna secretion. using phoa and green fluorescent protein (gfp) as periplasmic and cytoplasmic reporters, respectively, we demonstrate that virb3 contains two membrane-spanning domains and that both the n and c termini of the protein ... | 2010 | 20348257 |
| increasing anthraquinone production by overexpression of 1-deoxy-d: -xylulose-5-phosphate synthase in transgenic cell suspension cultures of morinda citrifolia. | a morinda citrifolia cell line was obtained by overexpresion of 1-deoxy-d: -xylulose 5-phosphate synthase (dxs) from catharanthus roseus, a key enzyme of the metabolic pathway of anthraquinones (aqs). this cell line increased aqs production by about 24% compared to the control cell line. this transgenic cell line which carries dxs cdna isolated from catharanthus roseus, was achieved by direct transformation of cell suspension cultures of m. citrifolia using a hypervirulent agrobacterium tumefaci ... | 2010 | 20349334 |
| molecular cloning and characterisation of cytoplasmic glutamine synthetase gene bcgs1 from non-heading chinese cabbage. | glutamine synthetase (gs; ec 6.3.1.2) is a key enzyme of nitrogen (n) assimilation, catalysing the synthesis of glutamine from ammonium and glutamate. plants have two types of gs isoenzyme that are localised in different compartments: one in the cytosol (gs1) and the other in the chloroplast (gs2). gs1 is the major form of gs in plant roots and directly converts ammonium taken up by plant roots to glutamine. | 2010 | 20355127 |
| agrobacterium tumefaciens-mediated transformation of maize endosperm as a tool to study endosperm cell biology. | developing maize (zea mays) endosperms can be excised from the maternal tissues and undergo tissue/cell-type differentiation under in vitro conditions. we have developed a method to transform in vitro-grown endosperms using agrobacterium tumefaciens and standard binary vectors. we show that both aleurone and starchy endosperm cells can be successfully transformed using a short cocultivation with a. tumefaciens cells. the highest transformation rates were obtained with the a. tumefaciens eha101 s ... | 2010 | 20357137 |
| baca is essential for bacteroid development in nodules of galegoid, but not phaseoloid, legumes. | baca is an integral membrane protein, the mutation of which leads to increased resistance to the antimicrobial peptides bleomycin and bac7(1-35) and a greater sensitivity to sds and vancomycin in rhizobium leguminosarum bv. viciae, r. leguminosarum bv. phaseoli, and rhizobium etli. the growth of rhizobium strains on dicarboxylates as a sole carbon source was impaired in baca mutants but was overcome by elevating the calcium level. while baca mutants elicited indeterminate nodule formation on pea ... | 2010 | 20363949 |
| the frankia alni symbiotic transcriptome. | the actinobacteria frankia spp. are able to induce the formation of nodules on the roots of a large spectrum of actinorhizal plants, where they convert dinitrogen to ammonia in exchange for plant photosynthates. in the present study, transcriptional analyses were performed on nitrogen-replete free-living frankia alni cells and on alnus glutinosa nodule bacteria, using whole-genome microarrays. distribution of nodule-induced genes on the genome was found to be mostly over regions with high synten ... | 2010 | 20367468 |
| [clinical and microbiological characteristics of rhizobium radiobacter infectious: six cases report and literature review]. | to explore the clinical and microbiological characteristics of rhizobium radiobacter infection, and therefore to provide information for the prevention and treatment of the disease. | 2010 | 20367947 |
| plant growth-promoting trait of rhizobacteria isolated from soil contaminated with petroleum and heavy metals. | three hundred and seventy-four rhizobacteria were isolated from the rhizosphere soil (rs) or rhizoplane (rp) of echinochloa crus-galli, carex leiorhyncha, commelina communis, persicaria lapathifolia, carex kobomugi, and equisetum arvense, grown in contaminated soil with petroleum and heavy metals. the isolates were screened for plant growth-promoting potential (pgpp), including indole acetic acid (iaa) productivity, 1-aminocyclopropane-1-carboxylic acid (acc) deaminase activity, and siderophore( ... | 2010 | 20372032 |
| light-induced conformational changes of the chromophore and the protein in phytochromes: bacterial phytochromes as model systems. | recombinant phytochromes agp1 and agp2 from agrobacterium tumefaciens are used as model phytochromes for biochemical and biophysical studies. in biliverdin binding phytochromes the site for covalent attachment of the chromophore lies in the n-terminal region of the protein, different from plant phytochromes. the issue which stereochemistry the chromophore adopts in the so-called pr and pfr forms is addressed by using a series of locked chromophores which form spectrally characteristic adducts wi ... | 2010 | 20373318 |
| aerobic biodegradation of a sulfonated phenylazonaphthol dye by a bacterial community immobilized in a multistage packed-bed bac reactor. | a microbial community able to aerobically degrade the azo dye acid orange 7 was selected from riparian or lacustrine sediments collected at sites receiving textile wastewaters. three bacterial strains, pertaining to the genera pseudomonas, arthrobacter, and rhizobium, constitute the selected community. the biodegradation of ao7 was carried out in batch-suspended cell culture and in a continuously operated multistage packed-bed bac reactor. the rapid decolorization observed in batch culture, join ... | 2010 | 20376575 |
| improvement of coenzyme q(10) production by increasing the nadh/nad(+) ratio in agrobacterium tumefaciens. | previously screened coq(10)-overproducing agrobacterium tumefaciens a603-35 showed a relatively high nadh/nad(+) ratio (1.1), as compared to parental strain c58 (0.2) when we increased the expression levels of nadh-generating enzymes. also, the intracellular nadh/nad(+) ratio showed a positive correlation with the coq(10) content in a603-35. overexpression of glyceraldehyde 3-phosphate dehydrogenase in a603-35 shifted the nadh/nad(+) ratio at 48 h from 0.8 to 1.2, and thus the coq(10) content in ... | 2010 | 20378960 |
| a tobamovirus expression vector for agroinfection of legumes and nicotiana. | the highest recombinant protein expression levels in plants have been achieved using tobacco mosaic virus (tmv) vectors via agroinoculation of the tobacco, nicotiana benthamiana. these vectors have been utilized for pharmaceutical protein production and also can serve as rapid gene expression screens for proteonomics. we have constructed a similar vector based on the legume-infecting tobamovirus, sunn hemp mosaic virus (shmv), by deleting the coat protein gene (shmv eliminate coat protein gene o ... | 2010 | 20380855 |
| rhizobium vignae sp. nov., a symbiotic bacterium isolated from multiple legume species. | a group of rhizobial strains isolated from nodules of multiple legume species grown in different geographical regions of china had identical 16s rrna genes. phylogenetic analysis based on the 16s rrna gene sequences showed that the novel strains formed a subclade in the genus rhizobium together with rhizobium galegae, rhizobium huautlense and rhizobium alkalisoli, with 99.8 % gene sequence similarity between the strains. the dna-dna relatedness values between the representative strain ccbau 051 ... | 2011 | 20382786 |
| rhizobium tubonense sp. nov., isolated from root nodules of oxytropis glabra. | four rhizobial strains, designated ccbau 85046(t), ccbau 85051, ccbau 85048 and ccbau 85049, isolated from root nodules of oxytropis glabra grown in tibet, china, were previously defined, using amplified 16s rrna gene restriction analysis, as a novel group within the genus rhizobium. to clarify their taxonomic position, these strains were further analysed and compared with reference strains of related bacteria using a polyphasic approach. the 16s rrna gene analysis showed that the four isolates ... | 2011 | 20382797 |
| challenging semi-bootstrapping molecular-replacement strategy reveals intriguing crystal packing of rhizavidin. | the structure of rhizavidin, the first dimeric member of the avidin family which maintains high affinity towards biotin, was determined to high resolution by semet sad. consequently, the structure of the rhizavidin-biotin complex was determined by molecular-replacement methods using the apo structure as the search model; this ran into complications and required combined programs as well as bootstrapping approaches. although present as a dimer in solution, rhizavidin packs as unique oligomers in ... | 2010 | 20383003 |
| metabolic engineering of glyoxalase pathway for enhancing stress tolerance in plants. | glyoxalase system consists of two enzymes glyoxalase i (gly i) and glyoxalase ii (gly ii). gly i detoxifies methylglyoxal (mg), a cytotoxic byproduct of glycolysis, to s-lactoylglutathione (slg) where it uses one molecule of reduced glutathione. subsequently, slg is converted to lactate by gly ii and one molecule of reduced glutathione is recycled back into the system. the level of mg, which is produced ubiquitously in all living organisms, is enhanced upon exposure to different abiotic stresses ... | 2010 | 20387042 |
| functional characterization of water-deficit stress responsive genes using rnai. | in response to water-deficit stress, plants alter expression of thousands of genes and as a result, cellular, physiological, and biochemical processes are modified. understanding the functional role of water-deficit stress-responsive genes is important in order to develop stress-tolerant plants. rna interference (rnai) technology is one of the potential reverse genetics tool for assessing the functional significance of these genes. we describe here the protocols for developing stable gene knockd ... | 2010 | 20387047 |
| [soil microbial properties under different vegetation types in loess hilly region]. | by using fumigation-extract (fe) method and biolog ecoplate, this paper investigated the microbial biomass and diversity in 0-20 cm soil layer under five vegetation types, including artificial woodland, shrubland, cropland, abandoned farmland, and natural grassland, in dingxi of gansu province. in the meanwhile, the relationships between soil microbes and soil nutrients were studied by path analysis, and the five typical vegetation types were evaluated from the aspect of soil microbes. relative ... | 2010 | 20387439 |
| [construction of t-dna insertion mutants of botrytis cinerea via agrobacterium tumefaciens mediated transformation and sequence analysis of insertion site]. | construction of botrytis cinerea t-dna insertion mutant library through agrobacterium-mediated transformation and for further understanding of the molecular mechanisms underlying the pathogenicity of botrytis cinerea. | 2010 | 20387458 |
| novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of xanthomonas fuscans subsp. aurantifolii. | citrus canker is a disease that has severe economic impact on the citrus industry worldwide. there are three types of canker, called a, b, and c. the three types have different phenotypes and affect different citrus species. the causative agent for type a is xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. xanthomonas fuscans subsp. aurantifolii strain b causes canker b and xanthomonas fuscans subsp. aurantifolii strain c causes canker c. | 2010 | 20388224 |
| mutation of a chloroplast-targeting signal in alternanthera mosaic virus tgb3 impairs cell-to-cell movement and eliminates long-distance virus movement. | cell-to-cell movement of potexviruses requires coordinated action of the coat protein and triple gene block (tgb) proteins. the structural properties of alternanthera mosaic virus (altmv) tgb3 were examined by methods differentiating between signal peptides and transmembrane domains, and its subcellular localization was studied by agrobacterium-mediated transient expression and confocal microscopy. unlike potato virus x (pvx) tgb3, altmv tgb3 was not associated with the endoplasmic reticulum, an ... | 2010 | 20392901 |
| transcriptional profiling of ests responsive to rhizobium vitis from 'tamnara' grapevines (vitis sp.). | genes related with defense responses were screened from the cdna library constructed with rhizobium vitis-inoculated or salicylic acid (sa)-treated 'tamnara' grapevine (vitis sp.) leaves. among 13,728 expressed sequence tags (ests) from 'tamnara' grapevine upon r. vitis inoculation and sa treatment, 6776 unigenes containing 1915 contigs and 4860 singletons were obtained. in gene ontology analysis, there were about 3200 clones related with biological process, 3555 with molecular function, and 335 ... | 2010 | 20398958 |
| a high-throughput colorimetric assay for screening halohydrin dehalogenase saturation mutagenesis libraries. | here we have reported a high throughput ph indicator-based assay to measure the activity of halohydrin dehalogenases (hhec). the assay relies upon the absorbance change at 560nm and the visual color change of phenol red in a weakly buffered system, due to the release of protons from the enzyme-catalyzed ring-closure reactions. the assay can be performed in a microplate format using whole cells, making the assay simple and robust. thus, it is suitable for library screening. the assay has been fur ... | 2010 | 20399816 |
| monomethylamine as a nitrogen source for a nonmethylotrophic bacterium, agrobacterium tumefaciens. | monomethylamine can be used by nonmethylotrophs as a sole nitrogen source but not as a carbon source; however, little is known about the genes and enzymes involved. the gamma-glutamylmethylamide/n-methylglutamate pathway for monomethylamine utilization by methylotrophs has recently been resolved. we have identified genes encoding key enzymes of this pathway in nonmethylotrophs (e.g., agrobacterium tumefaciens) and demonstrated that this pathway is also involved in the utilization of monomethylam ... | 2010 | 20400554 |
| phenolic acids act as signaling molecules in plant-microbe symbioses. | phenolic acids are the main polyphenols made by plants. these compounds have diverse functions and are immensely important in plant-microbe interactions/symbiosis. phenolic compounds act as signaling molecules in the initiation of legumerhizobia symbioses, establishment of arbuscular mycorrhizal symbioses and can act as agents in plant defense. flavonoids are a diverse class of polyphenolic compounds that have received considerable attention as signaling molecules involved in plant-microbe inter ... | 2010 | 20400851 |
| a role for psk signaling in wounding and microbial interactions in arabidopsis. | psk-alpha is a disulfated peptide that acts as a growth factor in plants. psk-alpha is derived from preproproteins which are encoded by five psk precursor genes in arabidopsis thaliana (l.) heynh and is perceived by leucine-rich repeat receptor kinases. arabidopsis has two psk receptor genes, pskr1 and pskr2. although ligand and receptor are well characterized, the biological functions of psk signaling are not well understood. using reporter lines and receptor knockout mutants of arabidopsis, a ... | 2010 | 20403122 |
| one-step, zero-background ligation-independent cloning intron-containing hairpin rna constructs for rnai in plants. | *the hairpin-based rna interference (rnai) technique plays an important role in exploring gene function in plants. although there are several methods for making hairpin rna (hprna) constructs, these methods usually need multiple relatively laborious, time-consuming or high-cost cloning steps. here we describe a one-step, zero-background ligation-independent cloning (oz-lic) method for making intron-containing hprna (ihprna) constructs by our vector prnai-lic. *to generate the ihprna constructs w ... | 2010 | 20406406 |
| study of polytopic membrane protein topological organization as a function of membrane lipid composition. | a protocol is described using lipid mutants and thiol-specific chemical reagents to study lipid-dependent and host-specific membrane protein topogenesis by the substituted-cysteine accessibility method as applied to transmembrane domains (scam). scam is adapted to follow changes in membrane protein topology as a function of changes in membrane lipid composition. the strategy described can be adapted to any membrane system. | 2010 | 20419405 |
| unveiling the intergenic transcriptome across rhizobium etli's lifecycle. | | 2009 | 20420221 |
| the small subunit arob of arsenite oxidase: lessons on the [2fe-2s] rieske protein superfamily. | here, we describe the characterization of the [2fe-2s] clusters of arsenite oxidases from rhizobium sp. nt-26 and ralstonia sp. 22. both reduced rieske proteins feature epr signals similar to their homologs from rieske-cyt b complexes, with g values at 2.027, 1.88, and 1.77. redox titrations in a range of ph values showed that both [2fe-2s] centers have constant e(m) values up to ph 8 at approximately +210 mv. above this ph value, the e(m) values of both centers are ph-dependent, similar to what ... | 2010 | 20421651 |
| isolation and partial characterization of bacterial strains on low organic carbon medium from soils fertilized with different organic amendments. | a total of 720 bacterial strains were isolated from soils with four different organic amendment regimes on a low organic carbon (low-c) agar medium (10 µg c ml(-1)) traditionally used for isolation of oligotrophs. organic amendments in combination with field history resulted in differences in dissolved organic carbon contents in these soils. there were negative correlations between total and dissolved organic carbon content and the number of isolates on low-c agar medium, whereas these correlati ... | 2010 | 20422409 |
| new approaches to enzymatic glycoside synthesis through directed evolution. | the expanding field of glycobiology requires tools for the synthesis of structurally defined oligosaccharides and glycoconjugates, while any potential therapeutic applications of sugar-based derivates would require access to substantial quantities of such compounds. classical chemical approaches are not well suited for such large-scale syntheses, thus enzymatic approaches are sought. traditional routes to the enzymatic assembly of oligosaccharides have involved the use of either nature's own bio ... | 2010 | 20427037 |
| the small heat-shock protein hspl is a virb8 chaperone promoting type iv secretion-mediated dna transfer. | agrobacterium tumefaciens is a plant pathogen that utilizes a type iv secretion system (t4ss) to transfer dna and effector proteins into host cells. in this study we discovered that an alpha-crystallin type small heat-shock protein (alpha-hsp), hspl, is a molecular chaperone for virb8, a t4ss assembly factor. hspl is a typical alpha-hsp capable of protecting the heat-labile model substrate citrate synthase from thermal aggregation. it forms oligomers in a concentration-dependent manner in vitro. ... | 2010 | 20427270 |
| impact of nutrient components on production of the phytoestrogens daidzein and genistein by hairy roots of psoralea corylifolia. | transformed hairy roots of psoralea corylifolia were established by infection with agrobacterium rhizogenes lba 9402. the aim of this work was to elucidate the effects of media constituents on production of the phytoestrogenic isoflavones daidzein and genistein. a. rhizogenes strain lba 9402 harboring ri plasmid was used to transform stem segments of in vitro seedlings. the resultant hairy roots were confirmed by polymerase chain reaction (pcr) and exhibited ri t-dna. transformed hairy root clon ... | 2010 | 20428956 |
| an efficient in vitro-inoculation method for tomato yellow leaf curl virus. | tomato yellow leaf curl virus (tylcv) is a member of the family geminiviridae, genus begomovirus. to test the infectivity of tylcv in tomato plants, an improved protocol for inoculation of in vitro-cultured tomato plants was developed. | 2010 | 20429892 |
| analysis of the mechanism of action of the antisense rna that controls the replication of the repabc plasmid p42d. | replication and segregation of the rhizobium etli symbiotic plasmid (pretcfn42d) depend on the presence of a repabc operon, which carries all the plasmid-encoded elements required for these functions. all repabc operons share three protein-encoding genes (repa, repb, and repc), an antisense rna (ctrna) coding gene, and at least one centromere-like region (pars). the products of repa and repb, in conjunction with the pars region, make up the segregation system, and they negatively regulate operon ... | 2010 | 20435728 |
| proteomic and transcriptomic characterization of a virulence-deficient phosphatidylcholine-negative agrobacterium tumefaciens mutant. | phosphatidylcholine (pc) is the most abundant phospholipid in eukaryotic membranes, whereas only a limited number of bacteria are able to synthesize pc. intriguingly, many of the bacteria with pc-containing membranes interact with eukaryotic hosts. pc is one of the major membrane lipids in the phytopathogenic bacterium agrobacterium tumefaciens. the presence of pc is critical for diverse cellular processes like motility, biofilm formation, stress resistance, and virulence. the exact role of pc i ... | 2010 | 20437057 |
| fast technique for agrobacterium-mediated transient gene expression in seedlings of arabidopsis and other plant species. | genome sequencing has identified a massive number of uncharacterized genes in arabidopsis thaliana and several other plant species. to decipher these unknown gene functions, several transient expression assays have been developed as rapid and convenient alternatives to the lengthy creation of transgenic plants. as one of these transient assays, agrobacterium-mediated transformation harnesses the natural capability of agrobacterium to transfer foreign dna into plant cells with intact cell walls. ... | 2010 | 20439415 |
| agrobacterium-mediated transformation of euphorbia tirucalli callus. | in order to establish a basis for transformation technology in the petroleum plant euphorbia tirucalli, the callus of the plant was infected with agrobacterium, washed with distilled water, sterilized with distilled water containing 100 mg/l of carbenicillin, selected on solidified b5 medium containing 13 mg/l of g418 and 100 mg/l of carbenicillin, and then on solidified b5 medium containing 25 mg/l of g418 and 100 mg/l of carbenicillin for the transgenic calli, and then the callus lines were su ... | 2010 | 20445322 |
| lotus endemic to the canary islands are nodulated by diverse and novel rhizobial species and symbiotypes. | genetic and symbiotic characterization of 34 isolates from several lotus species endemic to the canary islands showed extraordinary diversity, with bacteria belonging to different species of the genera mesorhizobium (17 isolates), sinorhizobium (12 isolates) and rhizobium/agrobacterium (5 isolates). in a previous report, we showed that the sinorhizobium isolates mostly belonged to s. meliloti. here, we focused on the remaining isolates. the lotus mesorhizobial strains were distributed in the rrs ... | 2010 | 20447791 |
| resonance assignments of the nucleotide-free wildtype mlok1 cyclic nucleotide-binding domain. | cyclic nucleotide-sensitive ion channels, known as hcn and cng channels play crucial roles in neuronal excitability and signal transduction of sensory cells. these channels are activated by binding of cyclic nucleotides to their intracellular cyclic nucleotide-binding domain (cnbd). a comparison of the structures of wildtype ligand-free and ligand-bound cnbd is essential to elucidate the mechanism underlying nucleotide-dependent activation of cnbds. we recently reported the solution structure of ... | 2010 | 20449776 |
| sinorhizobium versus ensifer: may a taxonomy subcommittee of the icsp contradict the judicial commission? | in response to a request for an opinion, the judicial commission (opinion 84) confirmed the earlier synonym, ensifer, as the correct name for the genus better known as sinorhizobium. the judicial commission considered that this decision would not cause sufficient confusion to justify the conservation of the latter name. the subcommittee on the taxonomy of agrobacterium and rhizobium of the international committee on systematics of prokaryotes (icsp) publicly disagreed with this conclusion. was i ... | 2010 | 20453103 |
| rhizobium borbori sp. nov., aniline-degrading bacteria isolated from activated sludge. | three aniline-degrading bacteria, strains dn316(t), dn316-1 and dn365, were isolated from activated sludge. according to 16s rrna gene sequence-based phylogenetic analysis, the isolates belonged to the genus rhizobium, with rhizobium ( = agrobacterium) radiobacter lmg 140(t) as the closest relative, with 96.5 % sequence similarity. phylogenetic analysis of the representative strain dn316(t) using sequences of the glna, thrc and reca genes and the 16s-23s intergenic spacer region confirmed the ph ... | 2011 | 20453105 |
| characterization of the nifa-rpon regulon in rhizobium etli in free life and in symbiosis with phaseolus vulgaris. | the nifa-rpon complex is a master regulator of the nitrogen fixation genes in alphaproteobacteria. based on the complete rhizobium etli genome sequence, we constructed an r. etli cfn42 oligonucleotide (70-mer) microarray and utilized this tool, reverse transcription (rt)-pcr analysis (transcriptomics), proteomics, and bioinformatics to decipher the nifa-rpon regulon under microaerobic conditions (free life) and in symbiosis with bean plants. the r. etli nifa-rpon regulon was determined to contai ... | 2010 | 20453139 |
| agrobacterium rhizogenes-mediated transformation of a high oil-producing filamentous fungus umbelopsis isabellina. | the agrobacterium rhizogenes-mediated transformation procedure was developed by using the hygromycin b phosphotransferase gene (hph) as a selective marker for the oil-producing fungus umbelopsis isabellina. different conditions were combined to increase the transformation efficiency. the highest efficiency was obtained by using a. rhizogenes strain r105 and a vector with zygomycete promoter. southern blot analysis demonstrated that 71% of transformants contained random integrations of t-dna sequ ... | 2010 | 20453313 |
| [toxicological evaluation of biopreparations on the basis of nitrogen-fixing bacteria]. | a comparative analysis of results of toxicological research of microbiological preparations on the basis of different species of nitrogen-fixing microorganisms of azotobacter, agrobacterium, azospirillum general and pathogenic properties of strains-producers has been carried out. a possibility to improve methodical principles of toxicological estimation and hygienic regulation of associative nitrogen-fixing microorganisms-producers and preparations on their basis in the industrial objects and en ... | 2009 | 20455429 |
| characterization of swarming motility in rhizobium leguminosarum bv. viciae. | we have characterized swarming motility in rhizobium leguminosarum strains 3841 and vf39sm. swarming was dependent on growth on energy-rich media, and both agar concentration and incubation temperature were critical parameters for surface migration. a cell density-dependent lag period was observed before swarming motility was initiated. surface migration began 3-5 days after inoculation and a full swarming phenotype was observed 3 weeks after inoculation. the swarming front was preceded by a cle ... | 2010 | 20455952 |
| nodulation and nitrogen fixation by mimosa spp. in the cerrado and caatinga biomes of brazil. | *an extensive survey of nodulation in the legume genus mimosa was undertaken in two major biomes in brazil, the cerrado and the caatinga, in both of which there are high degrees of endemicity of the genus. *nodules were collected from 67 of the 70 mimosa spp. found. thirteen of the species were newly reported as nodulating. nodules were examined by light and electron microscopy, and all except for m. gatesiae had a structure typical of effective mimosa nodules. the endosymbiotic bacteria in nodu ... | 2010 | 20456044 |
| multiple evolutionary origins of legume traits leading to extreme rhizobial differentiation. | summary: *when rhizobia differentiate inside legume host nodules to become nitrogen-fixing bacteroids, they undergo a physiological as well as a morphological transformation. these transformations are more extreme in some legume species than others, leading to fundamental differences in rhizobial life history and evolution. here, we analysed the distribution of different bacteroid morphologies over a legume phylogeny to understand the evolutionary history of this host-influenced differentiation. ... | 2010 | 20456052 |
| mutation of outer-shell residues modulates metal ion co-ordination strength in a metalloenzyme. | the metal ion co-ordination sites of many metalloproteins have been characterized by a variety of spectroscopic techniques and small-molecule model systems, revealing many important insights into the structural determinants of metal ion co-ordination. however, our understanding of this fundamentally and practically important phenomenon remains frustratingly simplistic; in many proteins it is essentially impossible to predict metal ion specificity and the effects of remote 'outer-shell' residues ... | 2010 | 20459397 |
| expression of an immunogenic f1-v fusion protein in lettuce as a plant-based vaccine against plague. | yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. the development of an efficient and low-cost oral vaccine against these diseases is highly desirable. in this study, the immunogenic fusion protein f1-v from y. pestis was introduced into lettuce via agrobacterium-mediated transformation, and putative transgenic lines were developed. the presence of the transgene in these putative transgenic lines was determined using polymerase chain reaction (pcr), and transgen ... | 2010 | 20461403 |
| expression of the fluorescent proteins dsred and egfp to visualize early events of colonization of the chickpea blight fungus ascochyta rabiei. | ascochyta blight caused by the ascomycete fungus ascochyta rabiei, is a major biotic constraint of chickpea (cicer arietinum l.), resulting in disastrous crop losses worldwide. to study early stages of development and pathogenic mechanisms of the fungus, two binary vectors for the constitutive expression of the red fluorescent protein (dsred-express) and the green fluorescent protein (egfp1) were constructed. furthermore, we have developed an improved and highly reproducible agrobacterium tumefa ... | 2010 | 20461519 |
| the chitinase gene (bbchit1) from beauveria bassiana enhances resistance to cytospora chrysosperma in populus tomentosa carr. | the chinese white poplar (populus tomentosa carr.) is susceptible to infection by plant diseases which severely affect its growth and substantially decrease its economic value. a chitinase gene (bbchit1) from beauveria bassiana was introduced into chinese white poplar (populus tomentosa carr.) by agrobacterium-mediated transformation. the t-dna of plant transformation vector contained the beta-glucuronidase reporter gene (gus) under the control of camv 35s promoter and the neomycin phosphotransf ... | 2010 | 20464449 |
| an improved agrobacterium-mediated transformation system for the functional genetic analysis of penicillium marneffei. | we have developed an improved agrobacterium-mediated transformation (amt) system for the functional genetic analysis of penicillium marneffei, a thermally dimorphic, human pathogenic fungus. our amt protocol included the use of conidia or pre-germinated conidia of p. marneffei as the host recipient for t-dna from agrobacterium tumefaciens and co-cultivation at 28°c for 36 hours. bleomycin-resistant transformants were selected as yeast-like colonies following incubation at 37°c. the efficiency of ... | 2010 | 20465521 |
| 1-deoxynojirimycins with dansyl capped n-substituents as probes for morbus gaucher affected cell lines. | cyclization by double reductive amination of d-xylo-hexos-5-ulose with methyl 6-aminohexanoate gave (methoxycarbonyl)pentyl-1-deoxynojirimycin. reaction of the terminal carboxylic acid with n-dansyl-1,6-diaminohexane provided the corresponding chain-extended fluorescent derivative. by reaction with bis(6-dansylaminohexyl)amine, the corresponding branched di-n-dansyl compound was obtained. both compounds are strong inhibitors of d-glucosidases and could also be shown to distinctly improve, at sub ... | 2010 | 20471633 |
| novel functions of stomatal cytokinesis-defective 1 (scd1) in innate immune responses against bacteria. | eukaryotes employ complex immune mechanisms for protection against microbial pathogens. here, we identified scd1 (stomatal cytokinesis-defective 1), previously implicated in growth and development through its role in cytokinesis and polarized cell expansion (falbel, t. g., koch, l. m., nadeau, j. a., segui-simarro, j. m., sack, f. d., and bednarek, s. y. (2003) development 130, 4011-4024) as a novel component of innate immunity. in arabidopsis, scd1 is a unique gene encoding for the only protein ... | 2010 | 20472560 |
| genetic and metabolic divergence within a rhizobium leguminosarum bv. trifolii population recovered from clover nodules. | rhizobia are able to establish symbiosis with leguminous plants and usually occupy highly complex soil habitats. the large size and complexity of their genomes are considered advantageous, possibly enhancing their metabolic and adaptive potential and, in consequence, their competitiveness. a population of rhizobium leguminosarum bv. trifolii organisms recovered from nodules of several clover plants growing in each other's vicinity in the soil was examined regarding possible relationships between ... | 2010 | 20472725 |
| vip1: linking agrobacterium-mediated transformation to plant immunity? | agrobacterium tumefaciens is the most efficient vehicle used today for the production of transgenic plants and plays an essential role in basic scientific research and in agricultural biotechnology. previously, plant vire2-interacting protein 1 (vip1) was shown to play a role in agrobacterium-mediated transformation. recent reports demonstrate that vip1, as one of the bzip transcription factors, is also involved in plant immunity responses. agrobacterium is able to activate and abuse vip1 for tr ... | 2010 | 20473505 |
| high-yield production, refolding and a molecular modelling of the catalytic module of (1,3)-beta-d-glucan (curdlan) synthase from agrobacterium sp. | biosynthesis of the (1,3)-beta-d: -glucan (curdlan) in agrobacterium sp., is believed to proceed by the repetitive addition of glucosyl residues from udp-glucose by a membrane-embedded curdlan synthase (crds) [udp-glucose: (1,3)-beta-d: -glucan 3-beta-d: -glucosyltransferase; ec 2.4.1.34]. the catalytic module of crds (cm-crds) was expressed in good yield from a cdna encoding cm-crds cloned into the pet-32a(+) vector, containing a coding region for thioredoxin, and from the champion pet sumo sys ... | 2010 | 20473714 |
| rapid, high-yield production in plants of individualized idiotype vaccines for non-hodgkin's lymphoma. | animal and clinical studies with plant-produced single-chain variable fragment lymphoma vaccines have demonstrated specific immunogenicity and safety. however, the expression levels of such fragments were highly variable and required complex engineering of the linkers. moreover, the downstream processing could not be built around standard methods like protein a affinity capture. | 2010 | 20494963 |
| a high-conductance cation channel from the inner membrane of the free-living soil bacteria rhizobium etli. | in this communication we reported the study of a cation channel present in the cytoplasmic membrane of the nitrogen fixing bacterium rhizobium etli. inner-membrane (im) vesicles were purified and fused into planar lipid bilayers (plbs), under voltage clamp conditions. we have found that fusion of im-enriched vesicle fractions with these model membranes leads, mainly (>30% of 46 experiments), to the reconstitution of high-conductance channels. following this strategy, the activity of a channel wi ... | 2010 | 20502878 |
| interactions of botryococcus braunii cultures with bacterial biofilms. | unicellular microalgae generally grow in the presence of bacteria, particularly when they are farmed massively. this study analyzes the bacteria associated with mass culture of botryococcus braunii: both the planktonic bacteria in the water column and those forming biofilms adhered to the surface of the microalgal cells (∼10⁷-10⁸ culturable cells per gram microalgae). furthermore, we identified the culturable bacteria forming a biofilm in the microalgal cells by 16s rdna sequencing. at least eig ... | 2010 | 20502890 |
| crystal structure of exotype alginate lyase atu3025 from agrobacterium tumefaciens. | alginate, a major component of the cell wall matrix in brown seaweeds, is degraded by alginate lyases through a beta-elimination reaction. almost all alginate lyases act endolytically on substrate, thereby yielding unsaturated oligouronic acids having 4-deoxy-l-erythro-hex-4-enepyranosyluronic acid at the nonreducing end. in contrast, agrobacterium tumefaciens alginate lyase atu3025, a member of polysaccharide lyase family 15, acts on alginate polysaccharides and oligosaccharides exolytically an ... | 2010 | 20507980 |
| β-odap accumulation could be related to low levels of superoxide anion and hydrogen peroxide in lathyrus sativus l. | level of the neuroexcitatory β-n-oxalyl-l-α,β-diaminopropionic acid (β-odap) in grass pea (lathyrus sativus l.) varies with development and environmental stress. reactive oxygen species (ros) (mainly o(2)ⁱ- and h(2)o(2)) are frequently reported to play important roles in plant development and in response to various stresses. to investigate the possible inter-relationship between contents of β-odap and ros, grass pea leaves have been analyzed for contents of β-odap, o(2)ⁱ- and h(2)o(2). the resul ... | 2011 | 20510333 |
| factors affecting β-odap content in lathyrus sativus and their possible physiological mechanisms. | a neuroexcitatory non-protein amino acid, β-n-oxalyl-l-α,β-diaminopropionic acid (β-odap), present in the seeds of the hardy legume crop grass pea (lathyrus sativus l.), was considered responsible for human lathyrism. the levels of β-odap were reported to vary in different tissues during plant development, and to be affected by a wide range of environmental stresses. in this paper, dynamic changes in β-odap level at specific stages of plant development as well as the influences of various enviro ... | 2011 | 20510335 |
| improvement of phosphate solubilization and medicago plant yield by an indole-3-acetic acid-overproducing strain of sinorhizobium meliloti. | nitrogen (n) and phosphorus (p) are the most limiting factors for plant growth. some microorganisms improve the uptake and availability of n and p, minimizing chemical fertilizer dependence. it has been published that the rd64 strain, a sinorhizobium meliloti 1021 strain engineered to overproduce indole-3-acetic acid (iaa), showed improved nitrogen fixation ability compared to the wild-type 1021 strain. here, we present data showing that rd64 is also highly effective in mobilizing p from insolub ... | 2010 | 20511434 |
| influence of soil fumigation by methyl bromide and methyl iodide on rhizosphere and phyllosphere microbial community structure. | rhizosphere and phyllosphere microbial communities were evaluated on roots and leaves of growth chamber-grown lettuce (lactuca sativa (l.) cv. green forest) plants by culture-dependent and -independent methods after soil fumigation. denaturing gradient gel electrophoresis (dgge) with 16s rrna primers followed by cloning and sequencing was used to identify major rrna bands from the rhizosphere and phyllosphere. three weeks after fumigation, there were no differences (p = 0.16) in rhizosphere micr ... | 2010 | 20512733 |
| rhizobium kunmingense sp. nov., isolated from rhizosphere soil of camptotheca acuminata decne. | strain lxd30(t) was isolated from rhizosphere soil of a plant of the species camptotheca acuminata decne which is native to warm, humid stream banks in southern china. analysis of the 16s rrna gene sequence revealed that the gram-negative, rod-shaped bacterium fell within the realm of the genus rhizobium and was most closely related to rhizobium huautlense so2(t) (96.4% sequence similarity) and rhizobium cellulosilyticum lmg 23642(t) (96.4%). the isolate grew optimally at ph7.0 and 25-28 degrees ... | 2010 | 20513962 |
| lasr receptor for detection of long-chain quorum-sensing signals: identification of n-acyl-homoserine lactones encoded by the avsi locus of agrobacterium vitis. | bacterial biosensor strains have greatly facilitated the rapid discovery, isolation, and study of quorum-sensing systems. in this study, we determined the relative sensitivity of a lasr-based e. coli bacterial bioluminescence biosensor jm109 (psb1075) for 13 diverse long-chain n-acyl-homoserine lactones (ahls) including oxygen-substituted and -unsubstituted ahls containing 14, 16, and 18 carbons and with and without double bonds. furthermore, we show by bioassay, hplc, and gc/ms that four long-c ... | 2011 | 20514483 |
| production of pharmaceutical-grade recombinant aprotinin and a monoclonal antibody product using plant-based transient expression systems. | plants have been proposed as an attractive alternative for pharmaceutical protein production to current mammalian or microbial cell-based systems. eukaryotic protein processing coupled with reduced production costs and low risk for mammalian pathogen contamination and other impurities have led many to predict that agricultural systems may offer the next wave for pharmaceutical product production. however, for this to become a reality, the quality of products produced at a relevant scale must equ ... | 2010 | 20514694 |
| [induction and in vitro culture of wedelia trilobata hairy roots]. | to study the possibilities for improvement of the ornamental character and production of secondary metabolites by using wedelia trilobata hairy roots, we investigated the induction of w. trilobata l. hairy roots and its consumption changes of carbon resource, nitrogen resource, phosphate and calcium in the medium during liquid culture. the results showed that hairy roots could be incited from the cut edges of leaf explants 7 days after inoculation with agrobacterium rhizogenes atcc15834 and coul ... | 2010 | 20518352 |
| polyphasic analysis of the bacterial community in the rhizosphere and roots of cyperus rotundus l. grown in a petroleum-contaminated soil. | cyperus rotundus l. is a perennial herb which was found prevailing in an area in the northeast of brazil previously contaminated with petroleum. in order to enlarge the knowledge of microorganism-plant interaction in phytoremediation, the bacterial community present in the rhizosphere and roots of c. rotundus was evaluated by culture-dependent and molecular approaches. pcr-dgge analyses based on the 16s rrna gene showed that the profiles of bulk soil, rhizosphere and root samples had a high degr ... | 2010 | 20519908 |
| rapid and efficient identification of agrobacterium species by reca allele analysis: agrobacterium reca diversity. | the analysis of housekeeping reca gene sequences from 138 strains from 13 species or genomic species of agrobacterium, nine being biovar 1 genomospecies, and the others agrobacterium larrymoorei, agrobacterium rubi, agrobacterium sp. ncppb 1650, and agrobacterium vitis and one "former" agrobacterium species, rhizobium rhizogenes, led to the identification of 50 different reca alleles and to a clear delineation of the 14 species or genomospecies entirely consistent with that obtained by amplified ... | 2010 | 20521039 |
| composition of bacterial communities associated with a plant-parasitic nematode bursaphelenchus mucronatus. | bursaphelenchus mucronatus is a plant-parasitic nematode widely existing in eurasian pine forests. to analyze the diversity and role of bacteria associated with the nematode, culture-dependent and culture-independent methods were used to identify and characterize the composition of bacterial community. a total of 13 bacterial isolates were obtained from b. mucronatus by the culture-dependent method. sixty-four species of bacteria were identified from two 16s rdna clone libraries constructed from ... | 2011 | 20524117 |