| aeroallergens in dairy barns near cooperstown, new york and rochester, minnesota. | we sampled atmospheric barn air using a volumetric air sampler in ten barns near cooperstown, ny and six barns near rochester, mn, and, with radioimmunoassays, measured allergens of aspergillus fumigatus, thermoactinomyces vulgaris, micropolyspori faeni, short ragweed, rye grass group i pollen, alternaria (alt-1), dermatophagoides sp. lepidoglyphus destructor, common insect allergen, mouse urine, rat urine, and cattle epithelium. the most abundant allergen present was a. fumigatus followed by l. ... | 1989 | 2764367 |
| fluctuations of serum iga and its subclasses in allergic bronchopulmonary aspergillosis. | exacerbations of allergic bronchopulmonary aspergillosis (abpa) are characterized by chest roentgenographic infiltrates, peripheral blood eosinophilia, and elevation of total serum ige. also elevated are serum immunoglobulin antibodies directed against aspergillus fumigatus, ige-af, serum igg-af, and serum iga-af. we measured serum iga-af, iga1-af, and iga2-af by elisa to determine whether elevations of iga-af occurred before or during an exacerbation (like igg-af) or after (like ige-af). ten ex ... | 1989 | 2778243 |
| keratinophilic fungi from the duck nails in egypt. | the mycoflora of one hundred and sixty of duck nail samples, collected from the duck farm, faculty of agriculture, assiut university, were examined. using the soil plate technique and sabouraud's dextrose agar, thirty six species appertaining to twenty genera of keratinophilic fungi were isolated. chrysosporium was the genus most frequently isolated (50% of the samples). aspergillus and penicillium occurred in low incidences (13.1 and 12.5% of total samples). trichophyton rubrum was found to col ... | 1989 | 2778647 |
| [allergenic and antigenic activities of the osmophilic fungus wallemia sebi asthmatic patients]. | recently large amounts of wallemia sebi, a species of osmophilic fungi, have been detected in house dust by low water activity media. the allergenic activity of w. sebi was examined by skin prick tests and radioallergosorbent tests (rast) in 74 asthmatic patients (mean age 11.7, range 6-32). aspergillus fumigatus and house dust were used for comparison. in the skin prick tests, w. sebi extract, a. fumigatus extract and house dust extract elicited positive reactions in 4 (5.4%), 4 (5.4%) and 51 ( ... | 1989 | 2783035 |
| the aspergillus toxin restriction is a suitable cytotoxic agent for generation of immunoconjugates with monoclonal antibodies directed against human carcinoma cells. | the protein toxin restriction, isolated from the mould aspergillus restrictus, inactivates protein synthesis in eukaryotic cells by blocking the ribosome elongation cycle. this protein acts as a specific nuclease that cuts off a small fragment from the 28-s rrna. biochemical and biological characterization of this toxin indicated that it is a non-glycosylated polypeptide of mr 16836, exhibiting in cell-free systems a protein synthesis inhibition capacity similar to that of the ricin a chain. thi ... | 1989 | 2783572 |
| allergic bronchopulmonary aspergillosis does exist in brazil. | a case of allergic bronchopulmonary aspergillosis is presented. the patient had corticosteroid-dependent asthma, recurrent pulmonary infiltrates clearing with oral prednisone bursts, positive dual (immediate and late) skin test (prick and intradermal) reactivity to aspergillus fumigatus, 11% blood eosinophilia, elevated total serum ige, positive precipitating antibody against aspergillus fumigatus, and elevated specific serum antibodies to aspergillus fumigatus (positive ige and igg antibody ind ... | 1989 | 2802269 |
| [bacterial infections in pulmonary aspergilloma]. | 28 cavernoscopies were carried out in 26 patients. complete or partial removal of mycetomas was achieved in 18 patients with 20 caverns. under aerobic conditions aspergillus fumigatus was cultured from the 28 removed mycetomas in 22 cases (78.6%) and bacterial organisms in 20 (71.4%). bacteria were isolated from mycetomas that had demonstrable a. fumigatus organisms as well as from those that did not have them. in all infected mycetomas gram negative rods were cultured, in only 4 cases gram (+) ... | 1989 | 2813148 |
| value of bronchoalveolar lavage in the management of severe acute pneumonia and interstitial pneumonitis in the immunocompromised child. | the diagnostic value of 73 bronchoalveolar lavages was assessed in 67 immunocompromised children (aged 3 months to 16 years) with pulmonary infiltrates. thirty one children had primary and 19 secondary immune deficiency, 14 acquired immunodeficiency syndrome (aids), and three aids related complex. bronchoalveolar lavage was performed during fibreoptic bronchoscopy, under local anaesthesia in all but two. one or more infective agents was found in eight of 11 patients with severe acute pneumonia a ... | 1987 | 2827334 |
| a comparison of the efficacy of itraconazole, amphotericin b and 5-fluorocytosine in the treatment of aspergillus fumigatus endocarditis in the rabbit. | the efficacy of amphotericin b, 5-fluorocytosine and itraconazole was compared for the treatment of experimental rabbit aspergillus fumigatus endocarditis. therapy with amphotericin b or 5-fluorocytosine, at dosages of 3.0 and 35 mg/kg body weight respectively, failed to eradicate aspergillus from the cardiac vegetations in all but one of the animals tested; none of these animals survived for longer than nine treatment days. when similar doses of amphotericin and 5-fluorocytosine were administer ... | 1987 | 2828301 |
| leukotriene b4 generation by human neutrophils following igg-dependent stimulation. | it has previously been shown that human neutrophils generate substantial quantities of ltb4 when stimulated with the calcium ionophore, a23187, or with unopsonized zymosan. we now report that normal human neutrophils produced substantial quantities of ltb4 (measured by radioimmunoassay and validated by rp-hplc) when incubated with large non-phagocytosable igg-coated beads (sepharose 4b). ltb4 was identified in both the extra and intracellular compartments. the production of ltb4 was dependent up ... | 1987 | 2832306 |
| use of bronchoalveolar lavage in immunocompromised children with pneumonia. | because pneumonia in the immunocompromised host presents a diagnostic dilemma to the pediatrician, we prospectively studied the use of bronchoalveolar lavage (bal) with flexible fiberoptic bronchoscopy in 14 immunocompromised children with pneumonia over a 5-month period. the children received immunosuppressive agents because of organ transplants, cancer, or systemic lupus erythematosus. a diagnosis was made by bal in 10 (71%) of the 14 children. pathogenic organisms included pneumocystis carini ... | 1988 | 2845341 |
| simultaneous aspergillus endophthalmitis and cytomegalovirus retinitis after kidney transplantation. | we report a case of disseminated and intraocular aspergillus and cytomegalovirus (cmv) infection in a renal transplant recipient. aggressive management included reduction of the immunosuppressive medications, systemic and intraocular administration of antifungal agents, treatment with a systemic antiviral drug, and ophthalmic surgery. the clinical and histopathologic features of intraocular aspergillus and cytomegalovirus infections are discussed. the literature of intraocular mixed opportunisti ... | 1988 | 2852837 |
| studies on antifungal agents. 18. in vitro activity of 5,5-disubstituted-3-phenyl-3-(1h-imidazol-1-ylmethyl)-2-methy lisoxazol idines and related compounds. | the in vitro antifungal activity of a series of novel 5,5-disubstituted-3-phenyl-3-(1h-imidazol-1-ylmethyl)-2-methyli soxazolidines and related compounds against trichophyton rubrum, aspergillus fumigatus and candida albicans was studied. the screening was performed in solid agar cultures and some structure-activity relationships involving the c-5-substitution pattern are discussed. | 1988 | 2854041 |
| participation of cell-mediated immunity in allergic bronchopulmonary aspergillosis. | while ige and igg antibodies are thought to play important pathogenetic roles in allergic bronchopulmonary aspergillosis (aba), the microscopic lung picture would implicate cell-mediated immunity. patients with aba do not manifest delayed skin test reaction to aspergillus fumigatus (af). this has been attributed to inhibition of lymphokines by histamine locally released as a result of the immediate skin test reaction. we tested 5 aba patients for delayed hypersensitivity to af. an attempt was ma ... | 1987 | 2886438 |
| prevention of airborne fungal infection in immunocompromised patients. | | 1988 | 2896699 |
| production and characterization of murine monoclonal antibodies to histoplasma capsulatum yeast cell antigens. | four monoclonal antibodies (mabs) were produced by immunizing mice with a disrupted yeast cell homogenate of histoplasma capsulatum. mabs 1 and 2 reacted only with the yeast cell antigens of h. capsulatum and blastomyces dermatitidis, whereas mabs 3 and 4 showed broader cross-reactivity. mab 3 cross-reacted with b. dermatitidis, paracoccidioides brasiliensis, sporothrix schenckii, and candida albicans, and mab 4 cross-reacted with b. dermatitidis, c. albicans, coccidioides immitis, aspergillus f ... | 1989 | 2917790 |
| [clinical observations on nasosinus aspergillosis]. | | 1987 | 2955485 |
| cellular and humoral immune responses in experimental aspergillosis in mice. | invasive aspergillosis was established in a naive murine model. both humoral and cellular aspects of the immunological mechanism responded to invasion. circulating immune complexes containing igg in the infected group of animals were detected by both the c1q and conglutinin solid phase assays. attempts to identify these complexes as aspergillus antigen-specific complexes were unsuccessful. cellular responses, as measured by blastogenic activity and inhibition of migration of sensitized cells wer ... | 1987 | 2957876 |
| aspergillary mycetomas ("aspergillomas") of the lung. histopathologic and mycologic investigations in 45 cases. | | 1987 | 2958697 |
| [aspergillus spondylodiscitis]. | | 1987 | 2962114 |
| high-performance liquid chromatography of siderophores from fungi. | a reversed-phase hplc separation of iron(iii) chelates of 16 representative fungal siderophores including ferrichromes, coprogens and triacetylfusarinine c was established in order to investigate siderophore production of fungi. for comparison purposes, the widely used bacterial siderophore ferrioxamine b was included. culture filtrates of the fungi penicillium resticulosum, fusarium dimerum, aspergillus fumigatus and neurospora crassa were quantitatively analyzed for the presence of known and u ... | 1988 | 2978959 |
| neurological manifestations of the acquired immunodeficiency syndrome (aids): experience at ucsf and review of the literature. | in this review of the acquired immunodeficiency syndrome (aids), the authors have evaluated a total of 352 homosexual patients with aids or generalized lymphadenopathy managed at the university of california, san francisco (ucsf), between 1979 and 1984. of an initial unselected group of 318 patients, 124 (39%) were neurologically symptomatic, and one-third already had their neurological complaints at the time of presentation. an additional 210 aids patients with neurological symptoms have been r ... | 1985 | 2983051 |
| the action of itraconazole and ketoconazole on growth and sterol synthesis in aspergillus fumigatus and aspergillus niger. | growth and sterol synthesis of aspergillus fumigatus and a. niger were studied in control cultures and in the presence of ketoconazole or itraconazole, the latter compound being 100 times more growth inhibitory than the former. sterol synthesis is inhibited more rapidly than any visible fungal outgrowth. this inhibition results in an accumulation of 4,14 dimethyl- and 4,4',14 trimethylsterols. the presence of these membrane-disturbing sterols may result in a pertubation of membrane-bound enzyme ... | 1985 | 2986303 |
| mechanisms of resistance of aspergillus fumigatus conidia to killing by neutrophils in vitro. | despite the critical role for neutrophils in host defenses against invasive aspergillosis, previous studies have established that neutrophils are unable to kill resting conidia of aspergillus fumigatus. the mechanisms of resistance of the conidia were therefore investigated. electron microscopy studies showed the fusion of phagosomes containing a. fumigatus conidia with lysosomes of the neutrophil. resting conidia of a. fumigatus were then compared with those that had been preincubated in broth ... | 1985 | 2989387 |
| [experimental renal infection with aspergillus fumigatus in mice: a chemotherapeutic model of an organic mycosis]. | | 1985 | 2989682 |
| itraconazole treatment of murine aspergillosis. | icr mice were challenged with conidia of aspergillus fumigatus given either intranasally or intravenously, and treated beginning 1 day later with itraconazole, amphotericin b, or the vehicles for these two agents. mice challenged intravenously and treated with either antifungal drug had prolonged survival over controls, and had lower tissue counts in the kidneys than the controls. however, mice challenged intranasally had neither prolonged survival nor lower lung tissue counts than the controls. | 1985 | 2992106 |
| the activity of ketoconazole and itraconazole against aspergillus fumigatus in mixed cultures with macrophages or leukocytes. | | 1986 | 3012331 |
| [invasive aspergillar pneumopathy during the treatment of microcellular bronchial cancer. efficacy of early antifungal treatment]. | the invasive aspergillus pneumonias have been described particularly in chemotherapy for patients with haematological disorders. in respiratory disorders such cases are exceptional. the authors report a case of invasive aspergillus pneumonia, occurring during treatment of a small cell cancer; the rapid commencement of anti-fungal treatment by amphotericin "b" and flucytosine enabled an apparent cure of the tumour by radiotherapy and chemotherapy. the authors stress the difficulty of definitive d ... | 1985 | 3012720 |
| [clinical aspects of pulmonary aspergilloma (31 cases)]. | | 1985 | 3012754 |
| [cytomegalovirus-associated superinfection of the lung following kidney transplantation]. | in a kidney-transplant patient, there was superinfection of the lungs by klebsiella pneumoniae and legionella pneumophilia with multiple necroses in the course of a primary cytomegalovirus infection. in a later phase of the disease, there was an opportunistic colonization of the necrotic cavities and the adjacent lung tissue by aspergillus fumigatus and candida albicans. the cytomegalovirus infection led to a pronounced cellular immunosuppression in the patient. this favored superinfections by b ... | 1987 | 3026768 |
| degenerative changes in fungi after itraconazole treatment. | changes in morphogenetic behavior and structural degeneration after exposure to itraconazole are illustrated in candida albicans, cryptococcus neoformans, pityrosporum ovale, paracoccidioides brasiliensis, trichophyton rubrum, and aspergillus fumigatus. with the exception of p. ovale, primary alterations are seen at the cell periphery and the cytoplasmic vacuoles in which lipid-like vesicles assemble. these changes are usually accompanied by a marked increase in cell volume, impaired cell divisi ... | 1987 | 3027844 |
| early experience with itraconazole in vitro and in patients: pharmacokinetic studies and clinical results. | the efficacy of itraconazole, a new triazole antifungal agent, was studied in vitro and assessed in patients. the mics of itraconazole for 16 strains of aspergillus fumigatus were in the same range as those of amphotericin b: less than 0.09-0.36 microgram/ml vs. less than 0.09-0.78 microgram/ml, respectively. eight adult patients with systemic fungal infections were treated orally with 100-200 mg of itraconazole two times a day. a patient with relapsing histoplasmosis (histoplasma capsulatum var ... | 1987 | 3027850 |
| [aspergillosis following liver transplantation as a hospital infection]. | in the first four weeks after a liver transplantation, there was an invasive aspergillosis with a lethal course in three out of five patients who were treated postoperatively in the same room. the clinical symptoms were very different. one patient was asymptomatic, and the diagnosis could only be made by autopsy. in another patient, pulmonary symptoms, and in the third patient, cerebral symptoms were the most prominent. in the two latter patients, the infection was demonstrated in the sputum and ... | 1987 | 3028750 |
| efficacy of fluconazole (uk-49,858) against experimental aspergillosis and cryptococcosis in mice. | the efficacy of fluconazole, a new bis-triazole antifungal agent, was compared with that of orally administered ketoconazole and parenterally administered amphotericin b against aspergillus and cryptococcus infections in mice. fluconazole was 5-20-fold more active than ketoconazole against systemic aspergillosis and against systemic, intracranial and pulmonary cryptococcosis but was less active than amphotericin b. | 1987 | 3038824 |
| suppression of host defences by aspergillus fumigatus. | an important feature of the microbicidal action of phagocytic cells is their ability to produce reactive oxygen intermediates. in an attempt to identify the mechanisms by which the fungus aspergillus fumigatus resists normal host defences the effect of spores and spore diffusates of a fumigatus on the production of superoxide anion and hydrogen peroxide by primed rodent phagocytic cells has been measured. for comparison we have used the non-pathogenic fungus penicillium ochrochloron. production ... | 1987 | 3039682 |
| oxidative killing of aspergillus fumigatus proceeds by parallel myeloperoxidase-dependent and -independent pathways. | the relative importance of several oxygen intermediates in fungicidal action against opsonized aspergillus fumigatus conidia was investigated with monocytes from normal volunteers and patients with either chronic granulomatous disease or myeloperoxidase (mpo) deficiency. results from experiments in which catalase, taurine, mannitol, or glucose-glucose oxidase were added to these phagocytes indicated that the mpo-hydrogen peroxide-halide system and an mpo-independent oxidative system exerted comp ... | 1987 | 3040589 |
| analysis of bronchoalveolar lavage in allergic bronchopulmonary aspergillosis: divergent responses of antigen-specific antibodies and total ige. | bronchoalveolar lavage (bal) was performed in eight patients with allergic bronchopulmonary aspergillosis (abpa) at a time when chest roentgeongraphy did not reveal an infiltrate, and respiratory status was stable. bal was tolerated well by all patients with only one patient experiencing mild wheezing. bal fluid recovery averaged 40%, and total cells/lavage were 22.3 x 10(6) (range 3.5 to 49.5 x 10(6)). cell viability, as determined by trypan blue exclusion, averaged 48% (range 34% to 60%). mean ... | 1988 | 3042837 |
| lectin-like attachment sites on murine pulmonary alveolar macrophages bind aspergillus fumigatus conidia. | murine pulmonary alveolar macrophages bound aspergillus fumigatus conidia in vitro at 4 c and 37 c in the absence of serum or opsonins. this attachment was dependent on calcium and was sensitive to mild trypsinization and paraformaldehyde pretreatment of the macrophage membrane. chitotriose, n-acetylglucosamine, d-mannose, alpha-methyl-mannoside, and l-fucose, but not d-galactose, were effective inhibitors of conidial binding. this pattern of reduction of conidial binding was consistent with tha ... | 1988 | 3042877 |
| effect of immunosuppression and amphotericin b on aspergillus antigenemia in an experimental model. | a modified enzyme-linked immunosorbent assay (elisa) for crude carbohydrate antigen was used to evaluate the kinetics of aspergillus antigenemia and to determine the effect of therapy on circulating antigen levels in an experimental model. the elisa was rapid, simple to perform, and able to detect less than 10 ng of antigen/ml of serum. the model was also used to evaluate the effect of temporary and persistent immunosuppression on experimental disease. antigen levels rose progressively in untrea ... | 1988 | 3042878 |
| production and characterization of a murine monoclonal antibody to aspergillus fumigatus antigen having igg- and ige-binding activity. | by employing hybridoma technology, a monoclonal antibody against aspergillus fumigatus was produced. this antibody, isotyped as igm k, reacted with 12 of 16 antigens extracted from 9 different a. fumigatus strains. this antibody also reacted with all 3 aspergillus flavus antigens studied, but not with aspergillus niger, aspergillus terreus, penicillium notatum or candida albicans antigens. western blot analysis indicated that this antibody reacted with two concanavalin a (con-a) binding bands of ... | 1988 | 3045013 |
| [mycologic analyses of house dust in patients with mycotic infections of the paranasal sinuses and in healthy probands]. | dust samples from 30 patients suffering from an aspergillus sinusitis and from 20 healthy persons were analysed for moulds as a possible cause of mycotic sinusitis. questionnaires were also distributed to assess the ecological environment of the patients. mycological analyses of the dust samples showed no statistical difference in the presence of moulds, especially those of aspergillus fumigatus and aspergillus niger. both moulds were found to roughly the same extent in 75%-95% of subjects. the ... | 1988 | 3045056 |
| [pseudo-allergic rhinitis in sinus aspergillosis. apropos of a case]. | | 1988 | 3045939 |
| igg antibodies to aspergillus fumigatus in cystic fibrosis: a laboratory correlate of disease activity. | serum was collected from 50 patients with cystic fibrosis, and igg antibodies to aspergillus fumigatus were measured by enzyme linked immunosorbent assay (elisa). in addition, total ige and aspergillus specific ige antibodies were measured in 41 of the 50. a close association was found between pulmonary function and clinical state, and igg antibodies to aspergillus. there was no association between pulmonary function or clinical state and ige antibodies. it is postulated that in patients with cy ... | 1988 | 3046514 |
| infection of the central nervous system in organ transplant recipients. | infections of the central nervous system are an important cause of morbidity and mortality in recipients of organ transplants. three organisms--listeria monocytogenes, aspergillus fumigatus, and cryptococcus neoformans--account for the great majority of such infections. the incidence of these infections is directly related to the patient's net state of immunosuppression and to the epidemiologic exposures he encounters. there is an expected timetable of when particular infections are likely to oc ... | 1988 | 3047541 |
| electroretinograms in early fungal endophthalmitis. | to facilitate the early diagnosis of exogenous fungal endophthalmitis, we developed a rabbit model for aspergillus fumigatus endophthalmitis. six eyes of six new zealand white rabbits were inoculated with forty spores of a. fumigatus. a control group of their six contralateral eyes received a similar but sterile inoculum. the rabbit eyes were evaluated with respect to clinical appearance and the electroretinogram. clinically evident endophthalmitis developed in all six infected eyes at an averag ... | 1988 | 3048943 |
| experimental aspergillosis in japanese quails (coturnix coturnix japonica). clinical signs and haematological changes. | intratracheal inoculation of 2-week-old quail chicks with aspergillus fumigatus resulted in the development of clinical signs within 24 h of infection. these were characterized by anorexia, depression, accelerated respiration and gasping followed by death. the acute course of the disease lasted for 7-10 days followed by recovery in the surviving chicks. the overall mortality during a 6-week observation period was 20%. although the mean body weight of a. fumigatus infected quail chicks continued ... | 1988 | 3050527 |
| studies on antifungal agents. 17. effects of the c-5-substitution on the in vitro activity of novel 3,5-substituted isoxazolidines. | a number of novel 5-(substituted thiomethyl)-3-phenyl-3-(1h-imidazol-1-ylmethyl)-2- methylisoxazolidine derivatives were prepared and evaluated in vitro for antifungal activity in solid agar cultures against trichophyton rubrum, aspergillus fumigatus and candida albicans. the effect of the 5-(substituted thiomethyl) group on the degree and scope of activity was studied and compared to that of the corresponding 5-phenoxymethyl and 5-[(substituted phenyl)thio(or amino)methyl] derivatives. | 1988 | 3053064 |
| [disseminated aspergillosis with marked morphological and functional defects of polymorphonuclear leukocytes in a case of myelodysplastic syndrome]. | | 1988 | 3054200 |
| effects of t-2 mycotoxin ingestion on phagocytosis of aspergillus fumigatus conidia by rabbit alveolar macrophages and on hematologic, serum biochemical, and pathologic changes in rabbits. | rabbits were given t-2 mycotoxin orally at 0, 0.25, 0.5, and 0.75 mg/kg of body weight/day for 21 days. only rabbits in the 0.75 mg/kg/day group (4 of 5 rabbits) died. alveolar macrophages were harvested on day 22 and used for in vitro phagocytosis of killed aspergillus fumigatus conidia. cultures included sera from untreated rabbits or rabbits treated with t-2. phagocytosis was significantly (p less than 0.01) reduced in cultures that used serum from rabbits treated with 0.5 mg of t-2/kg/day an ... | 1988 | 3056139 |
| [obstructive bronchial aspergillosis of favorable course under corticoids in a hiv seropositive patient]. | | 1988 | 3056177 |
| sequential pathological studies in japanese quails infected experimentally with aspergillus fumigatus. | intratracheal inoculation of young quail chicks with aspergillus fumigatus spores resulted in the development of characteristic gross and microscopic lesions. the lesions were restricted to respiratory tract and there was no dissemination of infection to other tissues of the body. gross changes in lungs and air sacs were observed within 24 hours and continued up to 20 days while in trachea these were noticed from the 3rd to the 9th day post-infection. the lesions, in general, included congestion ... | 1988 | 3057379 |
| a case of aspergillus osteomyelitis complicating an open fracture of the tibia. | | 1988 | 3058608 |
| serum antibodies to the catalase antigen of aspergillus fumigatus in cattle. | | 1988 | 3059782 |
| interferon protects mice against an extracellular infection of aspergillus fumigatus. | | 1988 | 3063178 |
| [a rare case of aspergilloma of the maxillary sinus]. | | 1988 | 3063937 |
| allergic bronchopulmonary aspergillosis: report of three cases. | | 1988 | 3067130 |
| brainstem invasion by aspergillus fumigatus in a child with leukemia. | | 1988 | 3068594 |
| surgery for pulmonary aspergilloma and pleural aspergillosis. | pulmonary aspergilloma and pleural aspergillosis are a potentially lifethreatening disease resulting from the colonization of lung or pleural cavities by the ubiquitous fungus aspergillus fumigatus. twenty four patients with pulmonary aspergilloma and five with pleural aspergillosis underwent major thoracic procedures at our hospital between 1976 and 1986. fourteen of the patients had haemoptysis, in 9 it was recurrent, and in 5 life-threatening. tuberculosis, pneumonia, and sarcoidosis were the ... | 1988 | 3068829 |
| an outbreak of disseminated aspergillosis associated with an intensive care unit. | | 1988 | 3069301 |
| pathologic, hematologic, and serologic changes in rabbits given t-2 mycotoxin orally and exposed to aerosols of aspergillus fumigatus conidia. | the influence of immunosuppression by t-2 mycotoxin on the fungal disease aspergillosis was investigated in rabbits. four groups of rabbits (groups 1a, 1b, 3a, and 3b) were given 0.5 mg of t-2 toxin/kg of body weight/day, po; in addition, rabbits of groups 3a and 3b were exposed to aerosols of aspergillus fumigatus conidia from days 7 through 16. rabbits of groups 2a and 2b were exposed to a fumigatus aerosols, but were not given t-2 toxin, and rabbits of group 0 served as controls. two rabbits ... | 1988 | 3071196 |
| binding of fibrinogen to the pathogenic aspergillus species. | the binding of human fibrinogen to the pathogenic aspergilli was investigated in vitro by different procedures using either fibrinogen in solution or fixed, insolubilized fibrinogen. binding of fibrinogen was detected at the surface of hyphae and conidia by an immunofluorescence assay. ultrastructural localization of the binding sites was visualized with fibrinogen-sensitized gold particles. the labelling was restricted to the outer cell wall layer of the 'smooth' walled conidia. quantitative an ... | 1988 | 3073204 |
| morphological studies on clinical isolates of aspergillus fumigatus. | morphological variation was examined in 19 strains of aspergillus fumigatus fres. isolated from the sputum of five patients with aspergilloma. for each patient a series of variant strains was obtained which exhibited gross differences in growth rate, colour and texture of the colony. on microscopical examination, some of the strains were found to produce markedly abnormal fruiting heads which were uncharacteristic for the species. scanning electron microscopy revealed that the variant produced c ... | 1988 | 3073205 |
| [biological activity of the soluble conidial proteins of aspergillus fumigatus fres. during germination]. | | 1988 | 3074256 |
| [toxigenicity and pathogenicity of micromycetes isolated from fish roe]. | | 1988 | 3074261 |
| invasive and granulomatous pulmonary aspergillosis with cerebral dissemination. | | 1988 | 3075268 |
| [invasive form of pulmonary aspergillosis in a patient with chronic lymphocytic leukemia successfully treated with amphotericin b]. | | 1988 | 3075740 |
| immunomodulation by candida albicans: crucial role of organ colonization and chronic infection with an attenuated agerminative strain of c. albicans for establishment of anti-infectious protection. | intravenous inoculation of an attenuated agerminative strain of candida albicans (pca-2) of low virulence, but not of two other species of candida of low virulence (c. parapsilosis and c. viswanathii) into cd2f1 mice conferred protection against the highly virulent microbes c. albicans ca-6, staphylococcus aureus and aspergillus fumigatus. to provide protection, a definite inoculum size (10(6) cells per mouse) resulting in organ colonization and establishment of a long-lasting chronic infection ... | 1988 | 3076189 |
| [pneumopathy caused by aspergillus in patients undergoing renal transplantation]. | | 1988 | 3077208 |
| [detection of specific antibodies in patients with aspergillosis using an immunoenzyme method]. | | 1988 | 3077714 |
| tremorgenic effects of mould extracts isolated in wood industry tested in rats. | | 1988 | 3078625 |
| pulmonary infection in cystic fibrosis. | | 1986 | 3090153 |
| significance of isolation of aspergillus from the respiratory tract in diagnosis of invasive pulmonary aspergillosis. results from a three-year prospective study. | the isolation of aspergillus species from respiratory secretions has been regarded as being of limited usefulness in the antemortem diagnosis of invasive pulmonary aspergillosis. one hundred and eight consecutive patients were evaluated in whom aspergillus species were isolated from respiratory secretions. invasive aspergillosis was not demonstrated in non-immunosuppressed patients or in patients with solid tumors in the absence of neutropenia. lung tissue was examined in 17 patients with leukem ... | 1986 | 3090879 |
| enzyme-linked immunosorbent assay in the detection of specific antibodies against aspergillus in patient sera. | a biotin-avidin linked enzyme immunoassay (balisa) was used to detect aspergillus fumigatus specific antibodies in the sera of patients with aspergillosis. culture filtrate antigens from a strain of a. fumigatus grown in synthetic medium was used in the elisa. this antigen was characterized by various immunochemical methods including isoelectric focusing, two-dimensional electrophoresis and antigen antibody crossed-immunoelectrophoresis. sera from patients having allergic asthma, allergic bronch ... | 1986 | 3094289 |
| selective partitioning of conidia of some penicillium and aspergillus species in aqueous two-phase systems. | conidia of aspergillus fumigatus, aspergillus flavus, aspergillus niger, penicillium brevi-compactum, penicillium frequentans, penicillium spinulosum, and penicillium verrucosum var. cyclopium were subjected to partition at varying ph values in an aqueous two-phase system containing charged polyethylene glycol. in the system, the partition behavior of the conidia of the penicillium species varied when the ph was raised, while the conidia of the aspergillus species seemed unaffected. p. brevi-com ... | 1986 | 3096203 |
| enzyme profile and immunochemical characterization of aspergillus fumigatus antigens. | we have compared the immunochemical characteristics of culture-filtrate antigens (ag) from aspergillus fumigatus extracted in our laboratory with commercially available ags. a total of 20 different preparations were studied for protein and carbohydrate content, presence of endotoxins, mycotoxins, and hemolytic toxins. these extracts were analyzed by two-dimensional electrophoresis for protein components. the immunogenicity of the preparations was determined by rocket electrophoresis with rabbit ... | 1986 | 3097110 |
| pulmonary aspergilloma. results of surgical treatment. | between 1953 and 1984, 53 patients (40 male and 13 female) underwent thoracotomy for treatment of pulmonary aspergilloma. the median age was 58 years (range 4 to 86 years). either underlying lung disease or immunologic risk factors were present in 49 patients (92%). twenty-one patients (31%) had simple aspergilloma and 32 (47%) had complex aspergilloma. the most common indication for operation was an indeterminate mass, hemoptysis, or severe cough. lobectomy, wedge excision, and pneumonectomy we ... | 1986 | 3097424 |
| galactomannan antigenemia and antigenuria in aspergillosis: studies in patients and experimentally infected rabbits. | purified galactomannan (gm) from aspergillus fumigatus was used in both a radioimmunoassay and an enzyme-linked immunoassay for antigen detection. results of the two tests seemed interchangeable. by one or both assays, gm was detected in serum from four of 12 rabbits lethally infected with a. fumigatus in concentrations ranging from 108 to 356 ng/ml. serum antigen was detected in only two of 12 patients with invasive aspergillosis. results of assay for gm in urine were far more encouraging. urin ... | 1987 | 3098861 |
| [amphotericin b and flucytosine therapy of aspergillus pneumonia and acute renal failure]. | kidney failure and pneumonia by aspergillus flavus and a. fumigatus were found in a 56-year-old woman who had received antibiotic and corticoid treatment to control high fever. her bloody tracheal secretion was a suspension of granule-like spore-free colonies of both aspergillus species. hemorrhages in mucous membranes and skin suggested a hematogenous dissemination of the fungi. aspergillus spores in the soil of ornamental plants were assumed to be responsible for the inhalatory infection. the ... | 1987 | 3104658 |
| enzyme-linked immuno-filtration assay (elifa) for the detection of igg, igm, iga and ige antibodies against aspergillus fumigatus. | the enzyme-linked immuno-filtration assay (elifa) permits detection of serological precipitating systems preformed by immunoelectro-diffusion on cellulose acetate strips and simultaneous characterization of immunoglobulins g, m, a and e specific for antigens of aspergillus fumigatus. we selected 36 sera from 9 patients who were followed up regularly and who suffered from aspergilloma (5 cases), allergic bronchopulmonary aspergillosis (2 cases), aspergillus bronchitis and invasive aspergillosis ( ... | 1987 | 3110398 |
| botrytis cinerea: a study of the immunological properties during growth. incidence of antibodies against b. cinerea in a group of patients with aspergillosis. | when growing botrytis cinerea in sabouraud medium, different phases of growth could be recognized using ph, mycelium yield, and culture filtrate antigens as parameters of growth. immunological characteristics of the different phases (early, intermediate, and late) of growth were measured using ige and igg binding by enzyme allergosorbent test and enzyme-linked immunosorbent assay, respectively, and quantities of precipitating components (double immunodiffusion and rocket immunoelectrophoresis). ... | 1987 | 3112026 |
| physical and morphological characteristics of eucaryotic ribosomes and lipopolysaccharide complexes. | lipopolysaccharides (lps) from pasteurella multocida or brucella abortus were complexed with aspergillus fumigatus ribosomes by mixing and fixation for 3 days in 3.8% formaldehyde. to investigate the nature of their physical association, ribosomes, lps, and ribosome-lps complexes were (i) centrifuged in cscl gradients to determine buoyant densities, (ii) examined by electron microscopy, and (iii) monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. ribosomes were found to bind ... | 1987 | 3114145 |
| effects of endotoxin (lipopolysaccharides) on experimental aspergillosis in leukemic mice. | in an attempt to evaluate effects of bacterial endotoxin on systemic fungal infection, experimental systemic aspergillus infection was induced in leukemic mice (akr strain) with (group b) or without (group a) preceding intraperitoneal administration of pseudomonas aeruginosa derived lipopolysaccharides. all mice in group a died within 4 days after intravenous inoculation of aspergillus fumigatus spores and developed extensive and disseminated fungal lesions without inflammatory reactions. in con ... | 1987 | 3116430 |
| qualitative and quantitative microbiological analysis of sputa of 102 patients with cystic fibrosis. | a microbiological analysis of 102 patients suffering from cystic fibrosis was conducted over a 22 month period. 20 microbial species with the following incidence were identified: pseudomonas aeruginosa: 83.4%; candida albicans: 29.4%; staphylococcus aureus: 24.5%; staphylococcus epidermidis: 11.8%; haemophilus influenzae: 11.8%; streptococcus pneumoniae; 6.9%; pseudomonas maltophilia: 6.8%; aspergillus fumigatus: 5.9%. other species were present in less than 5% of the patients. in the majority o ... | 1987 | 3117700 |
| [solid phase radioimmunoassay for the quantitative determination of antigen-specific igg-antibodies in the serum of patients with farmer's lung]. | in this study we have investigated a number of technical aspects of a sensitive solid-phase radioimmunoassay (microtitre-plate ria) for the quantitative determination of antigen-specific igg antibodies in the sera of patients suffering from farmer's lung. microtitre-plates were coated with antigens of aspergillus fumigatus, micropolyspora faeni, aureobasidium pullulans, alternaria tenuis, thermoactinomyces vulgaris and hay. optimal antigen concentration and serum dilution were determined, the te ... | 1987 | 3121779 |
| detection of antibodies against aspergillus fumigatus: comparison between double immunodiffusion, elisa and immunoblot analysis. | the performance of elisa to detect igg and igm antibodies to aspergillus fumigatus has been evaluated in strongly precipitin-positive, weakly precipitin-positive and precipitin-negative patient sera, with immunoblot analysis as the confirmatory test. all strongly precipitin-positive sera contained increased igg titers and showed clearly positive immunoblot patterns. most of the weakly precipitin-positive sera contained elisa titers within the normal range established with sera of healthy blood d ... | 1988 | 3123399 |
| distribution of the genus aspergillus in hospital room air conditioners. | | 1987 | 3123413 |
| serum antibodies to aspergillus fumigatus catalase in patients with cystic fibrosis. | seven to ten percent of patients with cystic fibrosis had serum antibodies to the catalase antigen of aspergillus fumigatus in three cross-sectional surveys between 1977 and 1984. a total of 208 patients participated at least once, and the cumulated frequency of catalase antibodies in 94 patients included in all three surveys was 16%. the titre range was 1 to 16. the prevalence rate of aspergillus fumigatus in sputum was 50% for a 2.5-year observation period. catalase antibodies were strongly as ... | 1988 | 3132374 |
| adrenal mass in an immunocompromised man. | | 1988 | 3132469 |
| aspergillus in pulmonary infections. | | 1988 | 3133316 |
| mic and fungicidal activity of terbinafine against clinical isolates of aspergillus spp. | terbinafine and amphotericin b mics for 90% of strains tested were 1.6 and 0.4 micrograms/ml against aspergillus fumigatus (16 strains), 0.8 and 3.2 micrograms/ml against aspergillus flavus (10 strains), and 0.4 and 1.6 micrograms/ml against aspergillus niger (10 strains), respectively. for all species tested, the minimal inhibitory and fungicidal concentrations for 90% of strains of both drugs were identical and the inoculum size did not have a major effect on the results. | 1988 | 3134851 |
| leukotriene c4 generation from human eosinophils stimulated with igg-aspergillus fumigatus antigen immune complexes. | sepharose beads coated with igg stimulate eosinophils to produce leukotriene c4 (ltc4). this observation has been extended with specific immobilized igg/antigen immune complexes to elicit mediator generation. an extract of aspergillus fumigatus was covalently coupled to sepharose beads and incubated with the igg fraction of immune serum from patients with allergic bronchopulmonary aspergillosis. these beads elicited generation of 7.72 +/- 1.7 pmol of ltc4 immunoreactive material (n = 5) from 1 x ... | 1988 | 3139729 |
| dot-immunobinding assay in the detection of igg antibodies against farmer's lung antigens. | circulating antibodies against faenia rectivirgula, thermoactinomyces candidus, t. vulgaris and aspergillus fumigatus were studied in the sera of 14 clinically proven farmer's lung patients and 10 normal controls using three immunological methods. these methods were agar gel double diffusion (dd), biotin-avidin-linked immunosorbent assay (balisa) and dot-immunobinding assay (diba). agar gel diffusion, the least sensitive of the three methods, failed to detect antibodies in some of the patients, ... | 1988 | 3140016 |
| an investigation of the bactericidal and fungicidal effects of certain disinfectants by use of a capacity test. | the bactericidal and fungicidal effects of five disinfectants and one combination of two disinfectants were tested using a modified kelsey-sykes method in which living microorganisms suspended in a sterilized yeast suspension ("dirty" conditions) and in sterile distilled water ("clean" conditions) were added to the disinfectants in three stages. six bacteria and two fungal organisms were employed as test microbes. results showed that formaldehyde was virtually inactive at the dilution tested (1/ ... | 1988 | 3140847 |
| catabolism of single ring aromatic acids by four aspergillus species. | four species of the genus aspergillus, viz a. fumigatus, a. japonicus, a. niger and a. terreus, decarboxylated, demethoxylated and ring-cleaved aromatic compounds but to different extents. decarboxylation of vanillate occurred before ring-cleavage, which preceded the release of 14co2 from the methoxyl group. a large proportion of labelled carbon from the ring of ferulate and vanillate was found in particulate or trichloracetic acid precipitable material of homogenized fungal mycelium. the four a ... | 1988 | 3144642 |
| sterilization potential of contact lens solutions. | | 1988 | 3148554 |
| isolation of aflatoxin from acacia and the incidence of aspergillus flavus in the sudan. | a total of 1300 seeds from 13 commodities were surveyed for aspergillus flavus. an additional inoculum was obtained from 6 divergent sources. a. flavus isolants, randomly selected on the bases of visual cultural characteristics and inoculum source, were assayed for aflatoxin yielding capacity. out of 97 analyzed isolates 76 (78.3%) were aflatoxin-elaborators of which 27 (27.8%) colonies were classified as very highly toxinogenic (greater than 1000 micrograms/kg). acacia, described a new source o ... | 1988 | 3148862 |
| serological diagnosis of aspergillus infections. | | 1988 | 3152082 |
| aspergillus fumigatus fungus ball in hospitalized patients with chronic pulmonary disease. usefulness of double immunodiffusion test as a screening procedure. | double immunodiffusion (did) was used as a screening test for the diagnosis of aspergillosis. three hundred and fifty patients were tested, all of them referred from a specialized chest disease hospital and without a definitive etiological diagnosis. when did was positive additional information such as clinical history and radiographic findings were requested and also surgical specimens were obtained whenever possible. specific precipitin bands for aspergillus fumigatus antigen were found in 29 ... | 1988 | 3152275 |
| neutrophil chemotactic responses induced by fresh and swollen rhizopus oryzae spores and aspergillus fumigatus conidia. | with the induction of germination, rhizopus oryzae spores and aspergillus fumigatus conidia activate the complement system and induce neutrophil chemotaxis. in contrast, freshly isolated r. oryzae spores did not induce neutrophil migration into lung tissue of mice after intranasal inoculation. moreover, in microchemotaxis assays neither fresh r. oryzae spores nor a. fumigatus conidia activated sera to stimulate human neutrophil chemotaxis above control migration until at least 10(7) or 10(8) spo ... | 1985 | 3157647 |
| studies on antifungal agents. 23. novel substituted 3,5-diphenyl-3-(1h-imidazol-1-ylmethyl)- 2-alkylisoxazolidine derivatives. | the synthesis and antifungal activity of a novel series of substituted 3,5-diphenyl-3-(1h-imidazol-1-ylmethyl)-2-alkylisoxazolidine derivatives (15-30) are described. the synthesis of the title compounds was accomplished via a 1,3-dipolar cycloaddition reaction of alpha-substituted ketonitrones with appropriate styrene precursors. the compounds when tested in vitro in solid agar cultures exerted a very potent antifungal activity against a wide variety of yeast and systemic mycoses and dermatophy ... | 1988 | 3172137 |