| comparison of the performance of 2 commercial multiplex pcr platforms for detection of respiratory viruses in upper and lower tract respiratory specimens. | the performance of the clart® pneumovir system with that of the luminex xtag rvp fast v1 assay for detection of most common respiratory viruses in upper and lower tract respiratory specimens (n=183) from unique patients with influenza-like syndrome or lower tract respiratory infection. nested pcr coupled to automated sequencing was used for resolution of discrepancies. fully concordant results were obtained for a total of 122 specimens, whereas 56 specimens gave partially (n=21) or fully discord ... | 2015 | 25724853 |
| first report of respiratory syncytial virus and human metapneumovirus co-infection in a 2-year-old kawasaki patient in iran. | respiratory virus infections in children are a leading cause of morbidity and mortality worldwide. | 2010 | 23113048 |
| association between respiratory infections in early life and later asthma is independent of virus type. | lower respiratory tract infections in the first years of life are associated with later asthma, and this observation has led to a focus on the potential causal role of specific respiratory viruses, such as rhinoviruses and respiratory syncytial virus, in asthma development. however, many respiratory viruses and bacteria trigger similar respiratory symptoms and it is possible that the important risk factors for asthma are the underlying susceptibility to infection and the exaggerated reaction to ... | 2015 | 25910716 |
| bacterial and viral etiology in hospitalized community acquired pneumonia with molecular methods and clinical evaluation. | polymerase chain reaction (pcr) method has improved the diagnosis rates for patients with community-acquired pneumonia (cap). we aimed to evaluate the bacterial and viral etiology of hospitalized cap cases and compare clinical and laboratory findings of patients with pure bacterial and bacterial and viral (mixed) infections. | 2014 | 24727518 |
| antibodies bound to aβ oligomers potentiate the neurotoxicity of aβ by activating microglia. | beta amyloid (aβ) oligomers are thought to contribute to the pathogenesis of alzheimer’s disease. however, clinical trials using aβ immunization were unsuccessful due to strong brain inflammation, the mechanisms of which are poorly understood. in this study we tested whether monoclonal antibodies to oligomeric aβ would prevent the neurotoxicity of aβ oligomers in primary neuronal-glial cultures. however, surprisingly,the antibodies dramatically increased the neurotoxicity of aβ. antibodies bound ... | 2013 | 23745639 |
| incidence and viral aetiologies of acute respiratory illnesses (aris) in the united states: a population-based study. | we conducted prospective, community-wide surveillance for acute respiratory illnesses (aris) in rochester, ny and marshfield, wi during a 3-month period in winter 2011. we estimated the incidence of aris in each community, tested for viruses, and determined the proportion of aris associated with healthcare visits. we used a rolling cross-sectional design to sample participants, conducted telephone interviews to assess ari symptoms (defined as a current illness with feverishness or cough within t ... | 2016 | 26931351 |
| etiology of acute bronchiolitis and the relationship with meteorological conditions in hospitalized infants in china. | to investigate the prevalence of common viruses and mycoplasma pneumoniae (mp) in hospitalized infants with acute bronchiolitis and study the relationship between bronchiolitis and meteorological conditions. | 2014 | 24961189 |
| prevalence and molecular characterization of human metapneumovirus in influenza a negative sample in thailand. | human metapneumovirus (hmpv) causes respiratory tract infection in influenza-like illness. the role of hmpv infections in all age groups in thailand has not yet been investigated. thus, the objective of this study was to determine prevalence of hmpv infection in all age groups in thailand during 2011. | 2014 | 24652781 |
| molecular viral epidemiology and clinical characterization of acute febrile respiratory infections in hospitalized children in taiwan. | acute respiratory infection (ari) is a leading cause of morbidity and hospitalization in children. to profile the viruses causing ari in children admitted to a community-based hospital in central taiwan, a cross-sectional study was conducted on children under 14 years of age that were hospitalized with febrile ari. viral etiology was determined using conventional cell culture and a commercial respiratory virus panel fast assay (xtag rvp), capable of detecting 19 different respiratory viruses and ... | 2015 | 26089293 |
| viral etiology of influenza-like illnesses in huizhou, china, from 2011 to 2013. | little information is available on the etiology and prevalence of viruses other than influenza viruses causing influenza-like illnesses (ilis) in china. this study was conducted for simultaneous detection and identification of 14 respiratory viruses in huizhou using real-time pcr. in total, viruses were detected in 48.66 % of ili patient samples, in which influenza virus (19.98 %) was the most commonly detected, followed by rhinovirus (7.46 %), human coronaviruses (3.63 %), human metapneumovirus ... | 2014 | 24610554 |
| epidemiology of respiratory viral infection using multiplex rt-pcr in cheonan, korea (2006-2010). | multiplex rt-pcr was used to detect respiratory viruses in 5,318 clinical samples referred to the laboratory of a tertiary teaching hospital from december 2006 to november 2010. the acquired data were analyzed with respect to types, ratio, and co-infection trends of infected respiratory viruses. trends in respiratory viral infection according to sex, age, and period of infection were also analyzed. of the 5,318 submitted clinical samples, 3,350 (63.0%) specimens were positive for at least one re ... | 2013 | 23412071 |
| risk factors of prolonged hospital stay in children with viral severe acute respiratory infections. | severe acute lower respiratory infections (saris) are one of the major causes of morbidity and mortality in young children, especially in developing countries. the present study focused on detection of risk factors for prolonged hospital stays among children with viral saris. | 2014 | 25313605 |
| evaluation of epidemiological and clinical features of influenza and other respiratory viruses. | in our study, we aimed to clinically and epidemiologically evaluate respiratory tract infections the viral agents of which were detected by molecular methods and to compare influenza and other respiratory tract viruses in this context. | 2015 | 26884691 |
| significant transplantation-related mortality from respiratory virus infections within the first one hundred days in children after hematopoietic stem cell transplantation. | respiratory viral infections (rvi) are important in hematopoietic stem cell transplantations (hsct) and knowledge regarding incidence, morbidity, mortality, and long-term pulmonary complications is limited. we report a study to evaluate incidence and outcomes, both short and long-term, of rvi in children receiving hsct. between january 2000 and december 2012, 844 patients underwent hematopoietic stem cell transplantation (hsct) at the hospital for sick children: 491 were allogeneic and 353 were ... | 2015 | 26117558 |
| viruses associated with pneumonia in adults. | viral pneumonia, which is typically associated with disease in childhood, is increasingly recognized as causing problems in adults. certain viruses, such as influenza virus, can attack fully immunocompetent adults, but many viruses take advantage of more-vulnerable patients. the latter include patients receiving immunosuppressive therapy and elderly subjects, particularly those residing in long-term care facilities. the range of viruses producing pneumonia in adults includes common agents, such ... | 2012 | 22423119 |
| [monitoring of viral pathogens in pediatric intensive care unit and analysis of clinical significance]. | to study the characteristics of viral spectrum and clinical features of children in pediatric intensive care unit (picu). | 2013 | 24131585 |
| [detection of respiratory viruses in influenza-like illness in shijiazhuang, china in 2011]. | this study aimed to investigate viral infections and the prevalence of influenza-like illness (ili) in shijiazhuang, china, in 2011 and to provide a scientific basis for the diagnosis and control of respiratory tract infections. throat swab specimens were collected from 483 cases of ili who were outpatients in the influenza surveillance sentinel hospitals in shijiazhuang between january and december 2011. all specimens were examined by multiplex rt-pcr for the following 15 respiratory tract viru ... | 2014 | 25272592 |
| high incidence of multiple viral infections identified in upper respiratory tract infected children under three years of age in shanghai, china. | upper respiratory tract infection (urti) is a major reason for hospitalization in childhood. more than 80% of urtis are viral. etiological diagnosis of urtis is important to make correct clinical decisions on treatment methods. however, data for viral spectrum of urtis are very limited in shanghai children. | 2012 | 22970251 |
| characterization of human coronavirus etiology in chinese adults with acute upper respiratory tract infection by real-time rt-pcr assays. | in addition to sars associated coronaviruses, 4 non-sars related human coronaviruses (hcovs) are recognized as common respiratory pathogens. the etiology and clinical impact of hcovs in chinese adults with acute upper respiratory tract infection (urti) needs to be characterized systematically by molecular detection with excellent sensitivity. | 2012 | 22719912 |
| etiology and clinical characterization of respiratory virus infections in adult patients attending an emergency department in beijing. | acute respiratory tract infections (artis) represent a serious global health burden. to date, few reports have addressed the prevalence of respiratory viruses (rvs) in adults with artis attending an emergency department (ed). therefore, the potential impact of respiratory virus infections on such patients remains unknown. | 2012 | 22389685 |
| detection of 12 respiratory viruses by duplex real time pcr assays in respiratory samples. | different viruses can be responsible for similar clinical manifestations of respiratory infections. thus, the etiological diagnosis of respiratory viral diseases requires the detection of a large number of viruses. in this study, 6 duplex real-time pcr assays, using evagreen intercalating dye, were developed to detect 12 major viruses responsible for respiratory diseases: influenza a and b viruses, enteroviruses (including enterovirus spp, and rhinovirus spp), respiratory syncytial virus, human ... | 2015 | 26334289 |
| multiplex sybr green real-time pcr assay for detection of respiratory viruses. | it is often difficult for a physician to distinguish between viral and bacterial causes of respiratory infections and this may result in overuse of antibiotics. in many cases of community-acquired respiratory infections, clinicians treat patients empirically. the development of molecular methods for direct detection of viruses has been progressed recently. | 2015 | 26468358 |
| the prevalence of human bocavirus, human coronavirus-nl63, human metapneumovirus, human polyomavirus ki and wu in respiratory tract infections in kuwait. | the aim of this study was to investigate the prevalence of human coronavirus (hcov)-nl63, human metapneumovirus (hmpv), human bocavirus (boca), human polyomavirus ki (kiv) and human polyomavirus wu (wuv) in respiratory tract infections (rti) in kuwait. | 2015 | 25925246 |
| identification of new respiratory viruses in the new millennium. | the rapid advancement of molecular tools in the past 15 years has allowed for the retrospective discovery of several new respiratory viruses as well as the characterization of novel emergent strains. the inability to characterize the etiological origins of respiratory conditions, particularly in children, led several researchers to pursue the discovery of the underlying etiology of disease. in 2001, this led to the discovery of human metapneumovirus (hmpv) and soon following that the outbreak of ... | 2015 | 25757061 |
| viral etiology of respiratory infections in children in southwestern saudi arabia using multiplex reverse-transcriptase polymerase chain reaction. | to investigate 15 respiratory viruses in children with acute respiratory tract infections (artis) using multiplex reverse-transcriptase polymerase chain reaction (rt-pcr), and to analyze the clinical and epidemiological features of these viruses. | 2014 | 25399211 |
| respiratory virus detection in nasopharyngeal aspirate versus bronchoalveolar lavage is dependent on virus type in children with chronic respiratory symptoms. | the comparative yield of respiratory virus detection from nasopharyngeal aspirate (npa) versus bronchoalveolar lavage (bal) is uncertain. furthermore, the significance of virus detection and its relationship to lower airway neutrophilic inflammation is poorly studied. | 2013 | 24125830 |
| [virus detection in bronchoalveolar lavage fluid of 122 children with severe pneumonia]. | to investigate the prevalence of viral pathogen in children with severe pneumonia in hunan. | 2013 | 24044210 |
| [the epidemiological study of adenovirus in children with respiratory tract infections in nanjing area from 2010 to 2011]. | to investigate the epidemiological features and types of human adenoviruses (adv) in children with acute respiratory tract infection in nanjing area, china. nasopharyngeal aspirates and nasopharyngeal swabs were collected from 644 outpatients or hospitalized pediatric patients with arti at the children hospital of nanjing, jiangsu province, china, between august 2010 and july 2011. adenoviruses were identified and typed from the collected clinical specimens by nested-pcr based on the partial reg ... | 2012 | 23233929 |
| the role of infections and coinfections with newly identified and emerging respiratory viruses in children. | acute respiratory infections are a major cause of morbidity in children both in developed and developing countries. a wide range of respiratory viruses, including respiratory syncytial virus (rsv), influenza a and b viruses, parainfluenza viruses (pivs), adenovirus, rhinovirus (hrv), have repeatedly been detected in acute lower respiratory tract infections (lrti) in children in the past decades. however, in the last ten years thanks to progress in molecular technologies, newly discovered viruses ... | 2012 | 23102237 |
| incidence of viral respiratory pathogens causing exacerbations in adult cystic fibrosis patients. | respiratory infections caused by respiratory viruses are common in paediatric cystic fibrosis (cf) patients and are associated with increased morbidity. there is only little data on the incidence of viral respiratory pathogens causing exacerbations in the adult cf patient population. in this observational pilot study we show, by using molecular as well as conventional techniques for viral isolation, that during 1 y a viral pathogen could be isolated in 8/24 (33%) adult cf patients who presented ... | 2013 | 22992129 |
| infections in pediatrics: old and new diseases. | paediatric infectious diseases have been reported since hippocrates, and were always closely linked with the evolution of medicine. current insights and recommendations by the pediatric infectious diseases group of switzerland for the management of common paediatric diseases, such as acute otitis media, pharyngitis, and acute viral gastroenteritis are reviewed. the emergence of "old" vaccine-preventable diseases - pertussis and measles - is also discussed. furthermore, the impact of the appearan ... | 2012 | 22847721 |
| [viral etiology of 1165 hospitalized children with acute lower respiratory tract infection]. | to explore the viral etiology of acute low respiratory tract infection (alrti) among hospitalized children in changsha of hunan province of china. | 2012 | 22289748 |
| respiratory viruses within homeless shelters in marseille, france. | homeless shelters are identified as places where humans are at high risk of acquiring respiratory disease. we previously reported the prevalence of the main respiratory diseases affecting a population of homeless in marseille, france. here, we investigated the prevalence of 10 respiratory viruses in a similar homeless population during 2 successive winter seasons. | 2014 | 24499605 |
| clinical characteristics of children with viral single- and co-infections and a petechial rash. | children with petechial rash are more likely to undergo invasive diagnostics, to be treated with antibiotics for potential bacterial infection and to be hospitalized. however, viruses have also been associated with petechial rash. nonetheless, a systematic analysis of viral infections with modern available techniques as quantitative real-time polymerase chain reaction in the context of petechial rash is lacking. the purpose of this pediatric study was to prospectively uncover viral pathogens tha ... | 2013 | 23249918 |
| viral etiology of influenza-like illnesses in cameroon, january-december 2009. | no information is available on the viral etiology of upper respiratory tract infections in cameroon. | 2012 | 23169968 |
| epidemiology of acute lower respiratory tract infection in hiv-exposed uninfected infants. | increased morbidity and mortality from lower respiratory tract infection (lrti) has been suggested in hiv-exposed uninfected (heu) children; however, the contribution of respiratory viruses is unclear. we studied the epidemiology of lrti hospitalization in hiv-unexposed uninfected (huu) and heu infants aged <6 months in south africa. | 2016 | 27025960 |
| the role of influenza, rsv and other common respiratory viruses in severe acute respiratory infections and influenza-like illness in a population with a high hiv sero-prevalence, south africa 2012-2015. | viruses detected in patients with acute respiratory infections may be the cause of illness or asymptomatic shedding. | 2016 | 26741826 |
| human metapneumovirus-associated severe acute respiratory illness hospitalisation in hiv-infected and hiv-uninfected south african children and adults. | data on human metapneumovirus (hmpv)-associated severe acute respiratory illness (sari) are limited in settings with high human immunodeficiency virus (hiv) infection prevalence. | 2015 | 26209394 |
| epidemiology of viral-associated acute lower respiratory tract infection among children <5 years of age in a high hiv prevalence setting, south africa, 2009-2012. | data on the epidemiology of viral-associated acute lower respiratory tract infection (lrti) from high hiv prevalence settings are limited. we aimed to describe lrti hospitalizations among south african children aged <5 years. | 2015 | 25093972 |
| an investigation into the prevalence and outcome of patients admitted to a pediatric intensive care unit with viral respiratory tract infections in cape town, south africa. | to describe the prevalence and outcome of patients admitted to a pediatric intensive care unit with viral respiratory tract infections. | 2012 | 22596071 |
| human metapneumovirus infection in chronic obstructive pulmonary disease: impact of glucocorticosteroids and interferon. | human metapneumovirus (hmpv) infection is implicated in exacerbations of asthma and chronic obstructive pulmonary disease (copd). research into the pathogenesis of infection is restricted to animal models and information about hmpv replication and inflammatory and immune responses in human disease is limited. | 2017 | 28379462 |
| surveillance for respiratory infectious diseases caused by 6 common viruses in a recruit training site in the northern region of china. | recruit training sites are places with a high incidence of respiratory infectious diseases. effective surveillance for acute respiratory infectious diseases in a recruit training site is an important way to prevent disease outbreaks. | 2017 | 28373908 |
| clinical features of human metapneumovirus infection in ambulatory children aged 5-13 years. | we detected human metapneumovirus (hmpv) in 54 (5%) of 1055 children aged 5 to 13 years with acute respiratory illness (ari) identified by outpatient and emergency department surveillance between november and may 2003-2009. its clinical features were similar to those of hmpv-negative ari, except a diagnosis of pneumonia was more likely (13% vs 4%, respectively; p = .005) and a diagnosis of pharyngitis (7% vs 24%, respectively; p = .005) was less likely in patients with hmpv- positive ari than th ... | 2017 | 28369564 |
| 180-nucleotide duplication in the g gene of human metapneumovirus a2b subgroup strains circulating in yokohama city, japan, since 2014. | human metapneumovirus (hmpv), a member of the family paramyxoviridae, was first isolated in 2001. seroepidemiological studies have shown that hmpv has been a major etiological agent of acute respiratory infections in humans for more than 50 years. molecular epidemiological, genetic, and antigenetic evolutionary studies of hmpv will strengthen our understanding of the epidemic behavior of the virus and provide valuable insight for the control of hmpv and the development of vaccines and antiviral ... | 2017 | 28352258 |
| simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time rt-pcr in patients with respiratory symptoms. | both respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) are important viral pathogens causing respiratory tract infection (rti) in the pediatric population. however, the clinical manifestations of rsv and hmpv infections are similar. therefore, a reliable and rapid diagnostic tool is needed for diagnostic performance. | 2017 | 28347279 |
| is therapeutic hypothermia for acute respiratory distress syndrome the future? | severe acute respiratory distress syndrome (ards) has a high mortality, and there is limited knowledge about management of severe ards refractory to standard therapy. early evidence suggests that therapeutic hypothermia (th) could be a viable treatment for acute respiratory failure. we present 2 cases where th was successfully used to manage refractory ards on extracorporeal membrane oxygenation (ecmo) and a review of the literature around th and acute respiratory failure. | 2017 | 28343415 |
| neutrophils regulate the lung inflammatory response via γδ t cell infiltration in an experimental mouse model of human metapneumovirus infection. | neutrophils are the most abundant leukocytes in human circulation. they are the first immune cell population recruited to the sites of infection. however, the role of neutrophils to regulate host immune responses during respiratory viral infections is largely unknown. to elucidate the role of neutrophils in respiratory antiviral defense, we used an experimental mouse model of human metapneumovirus (hmpv) infection. hmpv, a member of the paramyxoviridae family, is a leading respiratory pathogen c ... | 2017 | 28336678 |
| respiratory dna viruses are undetectable in nasopharyngeal secretions from adenotonsillectomized children. | respiratory viruses are frequently detected in association with chronic tonsillar hypertrophy in the absence of symptoms of acute respiratory infection (ari). the present analysis was done in follow-up to a previous clinical study done by this same group. nasopharyngeal washes (npws) were obtained from 83 of 120 individuals at variable times post adenotonsillectomy, in the absence of ari symptoms. a look back at virus detection results in npws from the same 83 individuals at the time of tonsille ... | 2017 | 28306724 |
| human metapnuemovirus infections in hospitalized children and comparison with other respiratory viruses. 2005-2014 prospective study. | human metapneumovirus (hmpv) has an important etiological role in acute lower respiratory infections in children under five years. our objectives were to estimate the relative contribution of hmpv to hospitalization in children with acute respiratory infection, to define the clinical and epidemiological features of hmpv single and multiple infections, and to compare hmpv infections with respiratory syncytial virus (hrsv), rhinovirus (hrv), adenovirus and human bocavirus infections in the same po ... | 2017 | 28301570 |
| influenza and other respiratory viruses: standardizing disease severity in surveillance and clinical trials. | influenza-like illness is a leading cause of hospitalization in children. disease burden due to influenza and other respiratory viral infections is reported on a population level, but clinical scores measuring individual changes in disease severity are urgently needed. areas covered: we present a composite clinical score allowing individual patient data analyses of disease severity based on systematic literature review and who-criteria for uncomplicated and complicated disease. the 22-item vivi ... | 2017 | 28277820 |
| expression of nkp46 splice variants in nasal lavage following respiratory viral infection: domain 1-negative isoforms predominate and manifest higher activity. | the natural killer (nk) cell activating receptor nkp46/ncr1 plays a critical role in elimination of virus-infected and tumor cells. the ncr1 gene can be transcribed into five different splice variants, but the functional importance and physiological distribution of nkp46 isoforms are not yet fully understood. here, we shed light on differential expression of nkp46 splice variants in viral respiratory tract infections and their functional difference at the cellular level. nkp46 was the most predo ... | 2017 | 28261217 |
| nk-cell receptors nkp46 and ncr1 control human metapneumovirus infection. | natural killer (nk) cells are capable of killing various pathogens upon stimulation of activating receptors. human metapneumovirus (hmpv) is a respiratory virus, which was discovered in 2001 and is responsible for acute respiratory tract infection in infants and children worldwide. hmpv infection is very common, infecting around 70% of all children under the age of five. under immune suppressive conditions, hmpv infection can be fatal. not much is known on how nk cells respond to hmpv. in this s ... | 2017 | 28191644 |
| etiology of severe pneumonia in ecuadorian children. | in latin america, community-acquired pneumonia remains a major cause of morbidity and mortality among children. few studies have examined the etiology of pneumonia in ecuador. | 2017 | 28182741 |
| burden of human metapneumovirus infections in patients with cancer: risk factors and outcomes. | human metapneumovirus (hmpv) causes upper and lower respiratory tract infections (uris and lris, respectively) in healthy and immunocompromised patients; however, its clinical burden in patients with cancer remains unknown. | 2017 | 28178369 |
| other community respiratory viruses. | polymerase chain reaction-based diagnosis has become the standard for viral pneumonia and other respiratory tract infections. expansion of respiratory viral panels (rvps) outside of influenza and, possibly, respiratory syncytial virus has led to the ability to diagnose viral infections for which no approved specific antiviral treatment exists. careful clinical evaluation of the patient with a positive rvp is, therefore, critical given the limited repertoire of treatments. generic treatments with ... | 2017 | 28159160 |
| antiviral treatments. | most viral respiratory tract infections are caused by classic respiratory viruses, including influenza, respiratory syncytial virus, human metapneumovirus, parainfluenza, rhinovirus, and adenovirus, whereas other viruses, such as herpes simplex, cytomegalovirus, and measles virus, can opportunistically affect the respiratory tract. the m2 inhibitors, amantadine and rimantadine, were historically effective for the prevention and treatment of influenza a but all circulating strains are currently r ... | 2017 | 28159156 |
| clinical evaluation of the isothermal amplification assays for the detection of four common respiratory viruses in children with pneumonia. | respiratory viruses are recognized as serious causes of morbidity and mortality in lower respiratory tract infections in patients. isothermal amplification assays are increasingly used in their detection because of their rapidity, simplicity and cost-effectiveness, when compared to traditional molecular diagnostic methods. however, systematic assessment of these assays in the clinical settings is rarely reported. medline (pubmed) searches were done analysing subject headings and words in the abs ... | 2017 | 28155195 |
| a case of rapid progression of postoperative hyperthermia: dantrolene or not dilemma? | malignant hyperthermia (mh) is an extremely rare and life-threatening differential diagnosis of postoperative fever. we present an 8-month-old child scheduled for elective outpatient procedure who rapidly developed high fever, tachycardia, and respiratory acidosis shortly after transfer to the postanesthesia care unit. mh hotline expert recommended administering dantrolene, but there was no evidence of hypermetabolism or lactic acidosis. the patient remained clinically stable after admission to ... | 2017 | 28149827 |
| structural basis for antibody cross-neutralization of respiratory syncytial virus and human metapneumovirus. | respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) are two closely related viruses that cause bronchiolitis and pneumonia in infants and the elderly(1), with a significant health burden(2-6). there are no licensed vaccines or small-molecule antiviral treatments specific to these two viruses at present. a humanized murine monoclonal antibody (palivizumab) is approved to treat high-risk infants for rsv infection(7,8), but other treatments, as well as vaccines, for both viruses are ... | 2017 | 28134915 |
| viral influenza-like illnesses: dynamic interrelationships during the 2015-2016 influenza season in hospitalized patients. | in hospitalized children and adults, the temporal relationship of viruses causing influenza-like illnesses (ilis) and influenza has not been well described. during the 2015-2016 influenza season at our hospital, the dynamic interrelationships between ili viruses (human metapneumovirus, respiratory syncytial virus, human parainfluenza viruses 3 and 4, rhinoviruses/enteroviruses, and coronaviruses) and influenza a were characterized in 768 hospitalized children and adults. | 2017 | 28131642 |
| current developments and prospects on human metapneumovirus vaccines. | human metapneumovirus (hmpv) has become one of the major pathogens causing acute respiratory infections (ari) mainly affecting young children, immunocompromised patients, and the elderly. currently there are no licensed vaccines against this virus. areas covered: since the discovery of hmpv in 2001, many groups have focused on developing vaccines against this pathogen. this review presents the outcomes and perspectives derived from preclinical studies performed in cell cultures and animals as we ... | 2017 | 28116910 |
| rapid profiling of rsv antibody repertoires from the memory b cells of naturally infected adult donors. | respiratory syncytial virus (rsv) causes substantial morbidity and mortality in young children and the elderly. there are currently no licensed rsv vaccines, and passive prophylaxis with the monoclonal antibody palivizumab is restricted to high-risk infants in part due to its modest efficacy. although it is widely agreed that an effective rsv vaccine will require the induction of a potent neutralizing antibody response against the rsv fusion (f) glycoprotein, little is known about the specificit ... | 2016 | 28111638 |
| cerebrospinal fluid findings in an adult with human metapneumovirus-associated encephalitis. | | 2017 | 28098534 |
| human quarantine: toward reducing infectious pressure on chimpanzees at the taï chimpanzee project, côte d'ivoire. | due to their genetic relatedness, great apes are highly susceptible to common human respiratory pathogens. although most respiratory pathogens, such as human respiratory syncytial virus (hrsv) and human metapneumovirus (hmpv), rarely cause severe disease in healthy human adults, they are associated with considerable morbidity and mortality in wild great apes habituated to humans for research or tourism. to prevent pathogen transmission, most great ape projects have established a set of hygiene m ... | 2017 | 28095600 |
| comparing human metapneumovirus and respiratory syncytial virus: viral co-detections, genotypes and risk factors for severe disease. | it is unclarified as to whether viral co-detection and human metapneumovirus (hmpv) genotypes relate to clinical manifestations in children with hmpv and lower respiratory tract infection (lrti), and if the clinical course and risk factors for severe lrti differ between hmpv and respiratory syncytial virus (rsv). | 2017 | 28095451 |
| impact of a transition from respiratory virus shell vial to multiplex pcr on clinical outcomes and cost in hospitalized children. | while respiratory virus pcr panel (rvpp) is more expensive than shell vial (sv) cell culture, it has been shown to reduce unnecessary diagnostic procedures, decrease the inappropriate use of antimicrobials, and shorten the hospital length of stay (los). we therefore hypothesized that, for hospitalized children, rvpp would be associated with improved clinical outcomes but higher hospital charges than sv cell culture. we performed a retrospective cohort study of hospitalized children. multivariate ... | 2017 | 28067857 |
| antiviral therapy for respiratory viral infections in immunocompromised patients. | respiratory viruses (influenza, parainfluenza, respiratory syncytial virus, coronavirus, human metapneumovirus, and rhinovirus) represent the most common causes of respiratory viral infections in immunocompromised patients. also, these infections may be more severe in immunocompromised patients than in the general population. early diagnosis and treatment of viral infections continue to be of paramount importance in immunocompromised patients; because once viral replication and invasive infectio ... | 2017 | 28067078 |
| paramyxovirus outbreak in a long-term care facility: the challenges of implementing infection control practices in a congregate setting. | objective we report an outbreak of respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) infections in a dementia care ward containing 2 separately locked units (a and b) to heighten awareness of these pathogens in the older adult population and highlight some of the infection prevention challenges faced during a noninfluenza respiratory viral outbreak in a congregate setting. methods cases were defined by the presence of new signs or symptoms that included (1) a single oral tempera ... | 2017 | 28065183 |
| development of a multiplex rt-pcr for simultaneous diagnosis of human metapneumovirus (hmpv) and human respiratory syncytial virus (hrsv) from clinical specimens. | human metapneumovirus (hmpv) and human respiratory syncytial virus (hrsv) are ubiquitous respiratory viral pathogens. they belong to the family paramyxoviridae (subfamily pneumovirinae) and is responsible for acute respiratory tract infections in children, elderly and immunocompromised patients. we designed and tested a multiplex reverse transcriptase polymerase chain reaction (mrt-pcr) as a cost-effective alternative to real-time pcr and cell culture based detection for hmpv and hrsv. the newly ... | 2016 | 28004017 |
| viral pathogens among elderly people with acute respiratory infections in shanghai, china: preliminary results from a laboratory-based surveillance, 2012-2015. | acute respiratory infections (aris), with viral pathogens as the major contributors, are the most common illnesses worldwide, and increase the morbidity and mortality among the elderly population. the clinical and pathological features of elderly people with aris need to be identified for disease intervention. from january 1(st) , 2012 through december 31(st) , 2015, respiratory specimens from patients above 60 years old with aris were collected from the outpatient and inpatient settings of six ... | 2016 | 27943329 |
| aberrant t cell immunity triggered by human respiratory syncytial virus and human metapneumovirus infection. | human respiratory syncytial virus (hrsv) and human metapneumovirus (hmpv) are the two major etiological viral agents of lower respiratory tract diseases, affecting mainly infants, young children and the elderly. although the infection of both viruses trigger an antiviral immune response that mediate viral clearance and disease resolution in immunocompetent individuals, the promotion of long-term immunity appears to be deficient and reinfection are common throughout life. a possible explanation f ... | 2016 | 27911218 |
| human metapneumovirus in the preterm neonate: current perspectives. | premature birth (<37 weeks gestation) occurs in ~11% of all births in the us. these infants are at risk of chronic lung disease and respiratory conditions, including bronchopulmonary dysplasia. respiratory viruses are important causes of acute respiratory illness (ari) in preterm infants, leading to rehospitalization, increased health care burden, and long-term morbidity. human metapneumovirus (hmpv) is a paramyxovirus discovered in 2001 that is related to respiratory syncytial virus. epidemiolo ... | 2016 | 27891060 |
| viral respiratory infections in a nursing home: a six-month prospective study. | the knowledge on viral respiratory infections in nursing home (nh) residents and their caregivers is limited. the purpose of the present study was to assess and compare the incidence of acute respiratory infections (ari) in nursing home (nh) residents and staff, to identify viruses involved in ari and to correlate viral etiology with clinical manifestations of ari. | 2016 | 27814689 |
| [monoinfection of human metapneumovirus in cordoba: first clinical and epidemiological research in children with respiratory infection in 2011]. | human metapneumovirus (hmpv) rna virus discovered in 2001, is a pathogen associated with acute respiratory infection (ari) in children under 5 years; its prevalence ranges from 5-15%. in córdoba, it is not integrated into the viral research in patients with low ira (lari). | 2016 | 27805553 |
| serology enhances molecular diagnosis of respiratory virus infections other than influenza in children and adults hospitalized with community-acquired pneumonia. | both molecular and serological assays have been used previously to determine the etiology of community-acquired pneumonia (cap). however, the extent to which these methods are correlated and the added diagnostic value of serology for respiratory viruses other than influenza virus have not been fully evaluated. using data from patients enrolled in the centers for disease control and prevention (cdc) etiology of pneumonia in the community (epic) study, we compared real-time reverse transcription-p ... | 2017 | 27795341 |
| molecular epidemiology of human metapneumovirus in riyadh province, saudi arabia. | human metapneumovirus (hmpv) is an important cause of respiratory tract illness in children. two hmpv subgroups, a and b, and four genotypes, a1, a2, b1 and b2, have been identified. concurrent circulation of the different genotypes in yearly epidemics has been recorded globally, but not in saudi arabia. the current report was designed to study hmpv epidemiology in saudi children and to analyze the genetic diversity and circulation patterns. nasopharyngeal aspirates (n = 174) were collected from ... | 2016 | 27694746 |
| human metapneumovirus in haematopoietic stem cell transplantation recipients: a case series and review of the diagnostic and therapeutic approach. | human metapneumovirus (hmpv) is a paramyxovirus that causes respiratory tract infections ranging from mild upper airway infection to severe pneumonia. patients with haematological disease, especially haematopoietic stem cell transplantation (hsct) recipients, are more likely to develop more severe infections. we describe three cases of hmpv infection in hsct patients. the most reliable diagnostic procedure for hmpv is multiplex ligation-dependent probe amplification (mlpa) on a nasopharyngeal sw ... | 2016 | 27762221 |
| [acute respiratory infection caused by the human metapneumovirus: analysis of 39 cases]. | | 2016 | 27345851 |
| functional motifs responsible for human metapneumovirus m2-2-mediated innate immune evasion. | human metapneumovirus (hmpv) is a major cause of lower respiratory infection in young children. repeated infections occur throughout life, but its immune evasion mechanisms are largely unknown. we recently found that hmpv m2-2 protein elicits immune evasion by targeting mitochondrial antiviral-signaling protein (mavs), an antiviral signaling molecule. however, the molecular mechanisms underlying such inhibition are not known. our mutagenesis studies revealed that pdz-binding motifs, 29-demi-32 a ... | 2016 | 27743962 |
| maternal vitamin d supplementation during pregnancy and lactation to prevent acute respiratory infections in infancy in dhaka, bangladesh (mdari trial): protocol for a prospective cohort study nested within a randomized controlled trial. | early infancy is a high-risk period for severe acute respiratory infection (ari), particularly in low-income countries with resource-limited health systems. lower respiratory tract infection (lrti) is commonly preceded by upper respiratory infection (urti), and often caused by respiratory syncytial virus (rsv), influenza and other common community-acquired viral pathogens. vitamin d status is a candidate modifiable early-life determinant of the host antiviral immune response and thus may influen ... | 2016 | 27737646 |
| human metapneumovirus impairs apoptosis of nasal epithelial cells in asthma via hsp70. | asthmatics are highly susceptible to respiratory viral infections, possibly due to impaired innate immunity. however, the exact mechanisms of susceptibility are likely to differ amongst viruses. therefore, we infected primary nasal epithelial cells (necs) from adults with mild-to-moderate asthma, with respiratory syncytial virus (rsv) or human metapneumovirus (hmpv) in vitro and investigated the antiviral response. necs from these asthmatics supported elevated hmpv but not rsv infection, compare ... | 2017 | 27723652 |
| the presence of fever in adults with influenza and other viral respiratory infections. | we compared the rates of fever in adult subjects with laboratory-confirmed influenza and other respiratory viruses and examined the factors that predict fever in adults. symptom data on 158 healthcare workers (hcws) with a laboratory-confirmed respiratory virus infection were collected using standardized data collection forms from three separate studies. overall, the rate of fever in confirmed viral respiratory infections in adult hcws was 23·4% (37/158). rates varied by virus: human rhinovirus ... | 2017 | 27691995 |
| human metapneumovirus induces reorganization of the actin cytoskeleton for direct cell-to-cell spread. | paramyxovirus spread generally involves assembly of individual viral particles which then infect target cells. we show that infection of human bronchial airway cells with human metapneumovirus (hmpv), a recently identified paramyxovirus which causes significant respiratory disease, results in formation of intercellular extensions and extensive networks of branched cell-associated filaments. formation of these structures is dependent on actin, but not microtubule, polymerization. interestingly, u ... | 2016 | 27683250 |
| community acquired respiratory virus infections in cancer patients-guideline on diagnosis and management by the infectious diseases working party of the german society for haematology and medical oncology. | community acquired viruses (crvs) may cause severe disease in cancer patients. thus, efforts should be made to diagnose crv rapidly and manage crv infections accordingly. | 2016 | 27681877 |
| spirometry filters can be used to detect exhaled respiratory viruses. | respiratory viruses are very common in the community and contribute to the burden of illness for patients with chronic respiratory diseases, including acute exacerbations. traditional sampling methods are invasive and problematic to repeat. accordingly, we explored whether respiratory viruses could be isolated from disposable spirometry filters and whether detection of viruses in this context represented presence in the upper or lower respiratory tract. discovery (n = 53) and validation (n = ... | 2016 | 27669334 |
| respiratory viruses and influenza-like illness: epidemiology and outcomes in children aged 6 months to 10 years in a multi-country population sample. | better population data on respiratory viruses in children in tropical and southern hemisphere countries is needed. | 2017 | 27667752 |
| etiology and clinical characteristics of single and multiple respiratory virus infections diagnosed in croatian children in two respiratory seasons. | the aim of this study was to determine the causative agent of acute respiratory infection (ari) in hospitalized children, as well as investigate the characteristics of aris with single and multiple virus detection in two respiratory seasons. in 2010 and 2015, nasopharyngeal and pharyngeal swabs from a total of 134 children, admitted to the hospital due to ari, were tested using multiplex pcr. viral etiology was established in 81.3% of the patients. coinfection with two viruses was diagnosed in 2 ... | 2016 | 27656298 |
| multiple strategies reveal a bidentate interaction between the nipah virus attachment and fusion glycoproteins. | the paramyxoviral family contains many medically important viruses, including measles virus, mumps virus, parainfluenza viruses, respiratory syncytial virus, human metapneumovirus, and the deadly zoonotic henipaviruses hendra and nipah virus (niv). to both enter host cells and spread from cell to cell within infected hosts, the vast majority of paramyxoviruses utilize two viral envelope glycoproteins: the attachment glycoprotein (g, h, or hemagglutinin-neuraminidase [hn]) and the fusion glycopro ... | 2016 | 27654290 |
| suppression of human metapneumovirus (hmpv) infection by the innate sensing gene ceacam1. | the innate sensing system is equipped with prrs specialized in recognizing molecular structures (pamps) of various pathogens. this leads to the induction of anti-viral genes and inhibition of virus growth. human metapneumovirus (hmpv) is a major respiratory virus that causes an upper and lower respiratory tract infection in children. in this study we show that upon hmpv infection, the innate sensing system detects the viral rna through the rig-i sensor leading to induction of ceacam1 expression. ... | 2016 | 27634893 |
| clinical characteristics and viral load of respiratory syncytial virus and human metapneumovirus in children hospitaled for acute lower respiratory tract infection. | respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) are two common viral pathogens in acute lower respiratory tract infections (alrti). however, the association of viral load with clinical characteristics is not well-defined in alrti. to explore the correlation between viral load and clinical characteristics of rsv and hmpv in children hospitalized for alrti in lanzhou, china. three hundred and eighty-seven children hospitalized for alrti were enrolled. nasopharyngeal aspirates (n ... | 2017 | 27632796 |
| effect of sialidase fusion protein (das 181) on human metapneumovirus infection of hep-2 cells. | human metapneumovirus is an emerging cause of lower respiratory disease in infants, young children, and immunocompromised adults. we have previously demonstrated that human metapneumovirus infection is mediated by interaction of human metapneumovirus attachment (g) and/or fusion (f) proteins with cellular glycosaminoglycans. we report here the activity of an investigational sialidase fusion protein, das181, on human metapneumovirus infection of hep-2 cells. these results suggest that human metap ... | 2016 | 27620888 |
| engineering, structure and immunogenicity of the human metapneumovirus f protein in the postfusion conformation. | human metapneumovirus (hmpv) is a paramyxovirus that is a common cause of bronchiolitis and pneumonia in children less than five years of age. the hmpv fusion (f) glycoprotein is the primary target of neutralizing antibodies and is thus a critical vaccine antigen. to facilitate structure-based vaccine design, we stabilized the ectodomain of the hmpv f protein in the postfusion conformation and determined its structure to a resolution of 3.3 å by x-ray crystallography. the structure resembles an ... | 2016 | 27611367 |
| human metapneumovirus (hmpv) infection in immunocompromised children. | human metapneumovirus (hmpv) is a major cause of upper and lower respiratory tract infection (urti, lrti) in children. the prognosis of hmpv is unclear in immunocompromised patients. | 2016 | 27522636 |
| respiratory viral infections and coinfections in children with cancer, fever and neutropenia: clinical outcome of infections caused by different respiratory viruses. | respiratory viral infections in episodes of fever and neutropenia (fn) in children with cancer are not well characterized. we compared the clinical outcome of infections caused by different respiratory viruses (rvs) and by rv coinfection in this population. | 2016 | 27518750 |
| etiology, seasonality, and clinical characteristics of respiratory viruses in children with respiratory tract infections in eastern india (bhubaneswar, odisha). | acute respiratory tract infections (artis) are a leading cause of morbidity and mortality in young children in low and middle income countries. to analyse the overall burden of respiratory viruses responsible for artis in paediatrics population in eastern india, this study was performed. clinical information, demographic information and nasal/oral swabs were collected from 332 paediatric patients (aged from 1 month to 12 years old) with the symptoms of arti, enrolled from the outpatient departme ... | 2017 | 27509268 |
| metapneumovirus infections and respiratory complications. | acute respiratory tract infections (artis) are the most common illnesses experienced by people of all ages worldwide. in 2001, a new respiratory pathogen called human metapneumovirus (hmpv) was identified in respiratory secretions. hmpv is an rna virus of the paramyxoviridae family, and it has been isolated on every continent and from individuals of all ages. hmpv causes 7 to 19% of all cases of artis in both hospitalized and outpatient children, and the rate of detection in adults is approximat ... | 2016 | 27486733 |
| incidence and etiology of hospitalized acute respiratory infections in the egyptian delta. | acute respiratory infections (ari) are responsible for nearly two million childhood deaths worldwide. a limited number of studies have been published on the epidemiology of viral respiratory pathogens in egypt. | 2017 | 27458989 |
| codetection of respiratory syncytial virus in habituated wild western lowland gorillas and humans during a respiratory disease outbreak. | pneumoviruses have been identified as causative agents in several respiratory disease outbreaks in habituated wild great apes. based on phylogenetic evidence, transmission from humans is likely. however, the pathogens have never been detected in the local human population prior to or at the same time as an outbreak. here, we report the first simultaneous detection of a human respiratory syncytial virus (hrsv) infection in western lowland gorillas (gorilla gorilla gorilla) and in the local human ... | 2016 | 27436109 |
| pathogen profiles and molecular epidemiology of respiratory viruses in japanese inpatients with community-acquired pneumonia. | the etiological profile of viruses among adult patients with community-acquired pneumonia (cap) has not been characterized yet. the aim of this study was twofold: first, investigate the pathogen profiles and the molecular epidemiology of respiratory viruses among japanese cap patients; and second, explore the clinical significance of viral infections. | 2016 | 27424825 |
| modulation of host immunity by the human metapneumovirus. | globally, as a leading agent of acute respiratory tract infections in children <5 years of age and the elderly, the human metapneumovirus (hmpv) has gained considerable attention. as inferred from studies comparing vaccinated and experimentally infected mice, the acquired immune response elicited by this pathogen fails to efficiently clear the virus from the airways, which leads to an exaggerated inflammatory response and lung damage. furthermore, after disease resolution, there is a poor develo ... | 2016 | 27413096 |