| interclade recombination in porcine parvovirus strains. | a detailed analysis of the ns1/vp1vp2 genome region of the porcine parvovirus (ppv) strains isolated from vaccinated animals was performed. we found many inconsistencies in the phylogenetic trees of these viral isolates, such as low statistical support and strains with long branches in the phylogenetic trees. thus, we used distance-based and phylogenetic methods to distinguish de facto recombinants from spurious recombination signals. we found a mosaic virus in which the ns1 gene was acquired fr ... | 2012 | 22993191 |
| family-based linkage and association mapping reveals novel genes affecting plum pox virus infection in arabidopsis thaliana. | sharka is a devastating viral disease caused by the plum pox virus (ppv) in stone fruit trees and few sources of resistance are known in its natural hosts. since any knowledge gained from arabidopsis on plant virus susceptibility factors is likely to be transferable to crop species, arabidopsis's natural variation was searched for host factors essential for ppv infection. to locate regions of the genome associated with susceptibility to ppv, linkage analysis was performed on six biparental popul ... | 2012 | 22943366 |
| chloroplast protection in plum pox virus-infected peach plants by l-2-oxo-4-thiazolidine-carboxylic acid treatments: effect in the proteome. | sharka, a disease caused by plum pox virus (ppv), has a significant economic impact on fruit tree production. in this work, we analysed the effect of (2,1,3)-benzothiadiazole (bth) and l-2-oxo-4-thiazolidine-carboxylic acid (otc) on plant growth and virus content. otc reduced sharka symptom, stimulated plant growth and alleviated ppv-induced oxidative stress, indicated by a lack of changes in some oxidative stress parameters. ppv infection reduced chloroplast electron transport efficiency. howev ... | 2013 | 22913613 |
| cloning of the complete infectious cdna of the plum pox virus strain ppv-rec. | plum pox virus (ppv) is the causal agent of sharka, considered to be the most detrimental viral disease of prunus spp. worldwide. so far, several ppv strains have been recognized, three of them (ppv-d, ppv-m, and ppv-rec) having shown serious economic impact in the european area. infectious cdna clones of plant rna viruses are excellent tools for functional studies of viral genomes. preparation and use of ppv-d and ppv-m infectious clones have been previously reported. here we describe the const ... | 2012 | 22720703 |
| plant growth stimulation in prunus species plantlets by bth or otc treatments under in vitro conditions. | the effects of benzothiadiazole (bth) and l-2-oxothiazolidine-4-carboxylic acid (otc) on the growth and viral content of micropropagated, plum pox virus (ppv)-infected peach [(prunus persica (l.) batsch] 'gf305' plantlets were analyzed. low bth and otc concentrations resulted in a significant increase in the growth of gf305 peach and plum plants, with greater effects in ppv-infected than in healthy gf305 peach plantlets. neither bth nor otc reduced the virus content. in fact, the highest growth ... | 2012 | 22595304 |
| high titer and avidity of nonneutralizing antibodies against influenza vaccine antigen are associated with severe influenza. | the importance of neutralizing antibody in protection against influenza virus is well established, but the role of the early antibody response during the initial stage of infection in affecting the severity of disease is unknown. the 2009 influenza pandemic provided a unique opportunity for study because most patients lacked preexisting neutralizing antibody. in this study, we compared the antibody responses of 52 patients with severe or mild disease, using sera collected at admission. a microne ... | 2012 | 22573737 |
| evaluation of the genetic diversity of plum pox virus in a single plum tree. | genetic diversity of plum pox virus (ppv) and its distribution within a single perennial woody host (plum, prunus domestica) has been evaluated. a plum tree was triply infected by chip-budding with ppv-m, ppv-d and ppv-rec isolates in 2003 and left to develop untreated under open field conditions. in september 2010 leaf and fruit samples were collected from different parts of the tree canopy. a 745-bp nib-cp fragment of ppv genome, containing the hypervariable region encoding the cp n-terminal e ... | 2012 | 22521553 |
| virus variants with differences in the p1 protein coexist in a plum pox virus population and display particular host-dependent pathogenicity features. | subisolates segregated from an m-type plum pox virus (ppv) isolate, ppv-ps, differ widely in pathogenicity despite their high degree of sequence similarity. a single amino acid substitution, k109e, in the helper component proteinase (hcpro) protein of ppv caused a significant enhancement of symptom severity in herbaceous hosts, and notably modified virus infectivity in peach seedlings. the presence of this substitution in certain subisolates that induced mild symptoms in herbaceous hosts and did ... | 2012 | 22458641 |
| serum protein profiles as potential biomarkers for infectious disease status in pigs. | in veterinary medicine and animal husbandry, there is a need for tools allowing the early warning of diseases. preferably, tests should be available that warn farmers and veterinarians during the incubation periods of disease and before the onset of clinical signs. the objective of this study was to explore the potential of serum protein profiles as an early biomarker for infectious disease status. serum samples were obtained from an experimental pig model for porcine circovirus-associated disea ... | 2012 | 22439879 |
| pvx-potyvirus synergistic infections differentially alter microrna accumulation in nicotiana benthamiana. | in comparison to single infections, co-infection of nicotiana benthamiana with potato virus x (pvx) and potato virus y (pvy) or plum pox virus (ppv), resulted in increased systemic symptoms (synergism in pathology). previous studies have shown that virus infections affected the accumulation of various micrornas (mirnas) and mirna target genes. our studies revealed that double infection by pvx and pvy or ppv that produced the most severe symptoms in n. benthamiana altered accumulation of mir156, ... | 2012 | 22387565 |
| the 3'-proximal part of the plum pox virus p1 gene determinates the symptom expression in two herbaceous host plants. | three major strains of the plum pox virus (ppv) are the most important in europe: ppv-d, ppv-m, and ppv-rec. by combining the genomes of two different strains of ppv (ppv-d with ppv-rec; ppv-d with ppv-m), 20 inter-strain chimeric infectious clones (cicppv) were constructed. biological properties of cicppv were tested by inoculating them on different herbaceous host species susceptible to ppv. four of the seven species tested, exhibited visible symptoms. in nicotiana benthamiana all cicppv induc ... | 2012 | 22367316 |
| virulence determines beneficial trade-offs in the response of virus-infected plants to drought via induction of salicylic acid. | it has been hypothesized that plants can get beneficial trade-offs from viral infections when grown under drought conditions. however, experimental support for a positive correlation between virus-induced drought tolerance and increased host fitness is scarce. we investigated whether increased virulence exhibited by the synergistic interaction involving potato virus x (pvx) and plum pox virus (ppv) improves tolerance to drought and host fitness in nicotiana benthamiana and arabidopsis thaliana. ... | 2017 | 28718885 |