movement of bromacil and hexazinone in soils of hawaiian pineapple fields. | bromacil and hexazinone have been heavily used to control weeds in the pineapple fields in central oahu, hawaii, usa, since 1970s and 1980s, respectively. the last application prior to this study was at a rate of 0.6 kg active ingredient (a.i.) ha(-1) for hexazinone and 1.8 or 3.4 kg (a.i.) ha(-1) for bromacil in the six study fields during june-october 1998. soils were collected from 0 to 1860 cm below the surface in january-may 1999 to survey the residue profiles of the two herbicides. stratop ... | 2002 | 12430654 |
comparison of the nutrient content of fresh fruit juices vs commercial fruit juices. | to compare the types and quantities of carbohydrate, electrolytes, ph and osmolarity of fresh fruit juices and commercial fruit juices. | 2002 | 12403253 |
3d modeling and virtual endoscopy of the small bowel based on magnetic resonance imaging in patients with inflammatory bowel disease. | small bowel mri (mr imaging) is a new imaging modality that excellently depicts small intestine pathology in patients with inflammatory bowel disease. virtual endoscopy based on sectional imaging is a recently introduced technique to create endoscopy like views. the aim of this study was to evaluate the feasibility of virtual small bowel endoscopy based on mr imaging in patients with crohn disease. | 2002 | 12218449 |
opalescent and cloudy fruit juices: formation and particle stability. | cloudy fruit juices, particularly from tropical fruit, are becoming a fast-growing part of the fruit juice sector. the classification of cloud as coarse and fine clouds by centrifugation and composition of cloud from apple, pineapple, orange, guava, and lemon juice are described. fine particulate is shown to be the true stable cloud and to contain considerable protein, carbohydrate, and lipid components. often, tannin is present as well. the fine cloud probably arises from cell membranes and app ... | 2002 | 12180775 |
bromelain treatment alters leukocyte expression of cell surface molecules involved in cellular adhesion and activation. | bromelain is a natural proteinase preparation derived from pineapple stem that is marketed for oral use as a digestive aid and as an antiinflammatory agent. bromelain treatment in vitro has been previously shown to selectively remove certain cell surface molecules that may affect lymphocyte migration and activation. this study reports the effects of bromelain on a broad range of cell surface molecules and on lymphocytes, monocytes, and granulocytes under physiologically relevant conditions. in v ... | 2002 | 12165279 |
identification and occurrence of the novel alkaloid pentahydroxypentyl-tetrahydro-beta-carboline-3-carboxylic acid as a tryptophan glycoconjugate in fruit juices and jams. | the novel carbohydrate-derived beta-carboline, 1-pentahydroxypentyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid, was identified in fruit- and vegetable-derived products such as juices, jams, and tomato sauces. this compound occurred as two diastereoisomers, a cis isomer (the major compound) and a trans isomer, ranging from undetectable amounts to 6.5 microg/g. grape, tomato, pineapple, and tropical juices exhibited the highest amount of this alkaloid (up to 3.8 mg/l), whereas apple, bana ... | 2002 | 12137498 |
characterization of genomic sequence coding for bromelain inhibitors in pineapple and expression of its recombinant isoform. | bromelain inhibitor (bi) is a cysteine proteinase inhibitor isolated from pineapple stem (reddy, m. n., keim, p. s., heinrikson, r. l., and kézdy, f. j. (1975) j. biol. chem. 250, 1741-1750). it consists of eight isoinhibitors, and each isoinhibitor has a two-chain structure. in this study, the genomic dna has been cloned and found to encode a precursor protein with 246 amino acids (m(r) = approximately 27,500) containing three isoinhibitor domains (bi-iii, -vi, and -vii) that are 93% identical ... | 2002 | 12016215 |
total antioxidant and ascorbic acid content of fresh fruits and vegetables: implications for dietary planning and food preservation. | epidemiological evidence links high intake of ascorbic acid (aa) and other antioxidant micronutrients to health promotion. it would be useful to know the overall, or 'total' antioxidant capacity of foods, to establish the contribution of aa to this, and to assess how this information may translate into dietary intakes to meet the new us daily reference intake for aa. in this study, the total antioxidant capacity, as the ferric reducing-antioxidant power (frap) value, and aa content of thirty-fou ... | 2002 | 11898770 |
hydrogen isotopic profile in the characterization of sugars. influence of the metabolic pathway. | the site-specific natural hydrogen isotope ratios of plant metabolites determined by 2h nuclear magnetic resonance (snif-nmr method) can provide powerful criteria for inferring mechanistic and environmental effects on biosynthetic pathways. this work examines the potential of isotopic profiles for the main constituents of carbohydrates, glucose and fructose, to distinguish different photosynthetic pathways. an appropriate analytical strategy, involving three suitable isotopic probes, has been el ... | 2002 | 11879039 |
high-performance liquid chromatographic analysis of free amino acids in fruit juices using derivatization with 9-fluorenylmethyl-chloroformate. | a simple, rapid, and reliable reversed-phase high-performance liquid chromatographic method for the analysis of 16 amino acids of main interest in commercial fruit juices (pear, orange, grapefruit, pineapple, peach, and apricot) is described. no sample cleanup is required. the ph of the fruit juices is adjusted to alkaline value (8.5) using 200 mm borate buffer, then amino acid is converted to stable derivatives using 9-fluorenylmethyl-chloroformate. the excess of derivatization reagent is remov ... | 2002 | 11866381 |
a comparative study on diurnal changes in metabolite levels in the leaves of three crassulacean acid metabolism (cam) species, ananas comosus, kalanchoë daigremontiana and k. pinnata. | a comparative study on diurnal changes in metabolite levels associated with crassulacean acid metabolism (cam) in the leaves of three cam species, ananas comosus (pineapple), a hexose-utilizing species, and kalanchoë daigremontiana and k. pinnata, two starch-utilizing species, were made. all three cam species showed a typical feature of cam with nocturnal malate increase. in the two kalanchoë species, isocitrate levels were higher than citrate levels; the reverse was the case in pineapple. in th ... | 2002 | 11807138 |
water quality of a reservoir as affected by agriculture in the east of thailand: a preliminary study. | a preliminary study on the water quality of a reservoir, affected by agriculture, in the east of thailand was conducted during 1996-1997. monitoring water quality of a reservoir is important because the sloping lands surrounding the reservoirs are mainly utilized for cultivating cash crops (pineapple, cassava, etc). a lot of fertilizers and agrochemicals were applied to soil and crops which can polluted the water. the results from the preliminary studied will be applied for the monitoring of the ... | 2001 | 11724479 |
bromelain: biochemistry, pharmacology and medical use. | bromelain is a crude extract from the pineapple that contains, among other components, various closely related proteinases, demonstrating, in vitro and in vivo, antiedematous, antiinflammatory, antithrombotic and fibrinolytic activities. the active factors involved are biochemically characterized only in part. due to its efficacy after oral administration, its safety and lack of undesired side effects, bromelain has earned growing acceptance and compliance among patients as a phytotherapeutical ... | 2001 | 11577981 |
comparison of two cysteine endopeptidases from pseudananas macrodontes (morr.) harms (bromeliaceae). | the properties of two cysteine peptidases (macrodontain i and ii) isolated from fruits of pseudananas macrodontes have been compared. the enzymes showed optimum ph ranges near neutrality and were inhibited by e-64 and other cysteine peptidase inhibitors. molecular masses were 23459 and 23703 kda, the isoelectric points were 6.1 and 5.9, and the km values were 13.4 and 8.9 microm (bz-phe-val-arg-amc) for macrodontain i and ii, respectively. n-alpha-cbz-l-amino acid p-nitrophenyl esters were teste ... | 2001 | 11517945 |
quantification of l-ascorbic acid and total ascorbic acid in fruits and spinach by capillary zone electrophoresis. | a standard curve for the quantification of l-ascorbic acid (l-aa) by capillary zone electrophoresis (cze) was established, and the quantification of ascorbic acid and total ascorbic acid in fruits (lemon, sunkist, and pineapple) and spinach were performed using d-isoascorbic acid (d-iaa) as an internal standard. the minimum detection limits (mdls) for l-aa and d-iaa were determined to be 1 and 2 microg/ml, respectively, at 265 nm. dehydroascorbic acid (dhaa) in fruits and spinach was quantified ... | 2001 | 11386660 |
ferulic acid is esterified to glucuronoarabinoxylans in pineapple cell walls. | the ester-linkage of ferulic acid (mainly e) to polysaccharides in primary cell walls of pineapple fruit (ananas comosus) (bromeliaceae) was investigated by treating a cell-wall preparation with 'driselase' which contains a mixture of endo- and exo-glycanases, but no hydroxycinnamoyl esterase activity. the most abundant feruloyl oligosaccharide released was o-[5-o-(e-feruloyl)-alpha-l-arabinofuranosyl](1-->3)-o-beta-d-xylopyranosyl-(1-->4)-d-xylopyranose (faxx). this indicated that the ferulic a ... | 2001 | 11261585 |
method for the quantitative determination of 1-naphthaleneacetic acid in spiked canned pineapple samples by micelle-stabilized room temperature phosphorescence. | this paper presents a convenient method for the determination of 1-naphthaleneacetic acid (naa) plant growth regulator in spiked canned pineapple samples by micellar-stabilized room temperature phosphorescence with sodium dodecyl sulfate as a micellar medium, thallium nitrate as an external heavy atom, and sodium sulfite as an oxygen scavenger. a multivariate optimization approach using the type of central composite blocked cube-star design was carried out. the analytical curve of naa gives a li ... | 1998 | 10554277 |
blood glucose responses of diabetes mellitus type ii patients to some local fruits. | to determine the glucose responses of diabetes mellitus type ii subjects to fruits, four locally available fruits (containing 25 g of available carbohydrates per serving portion) of chico, mango, pineapple, and papaya were tested among ten type ii diabetic subjects, using wheat bread as the control. results of the in vivo test indicated that chico and mango had significantly lower (p ≤ 0.05) blood glucose areas compared to wheat bread. chico and mango also had a much lower glycemic index (gi), 5 ... | 2000 | 24394507 |
high-performance liquid chromatographic determination with photodiode array detection of ellagic acid in fresh and processed fruits. | a high-performance liquid chromatographic (hplc) procedure based on an isocratic elution with photodiode array detection has been developed for a simple and rapid determination of ellagic acid (ea) in fresh and processed fruits. the homogenized sample was refluxed with methanol and then the extract was refined using a solid-phase cartridge before hplc. we analyzed ea in 40 kinds of fresh fruits and 11 kinds of processed fruits by the developed method. ea was found in several berries, fueijoa, pi ... | 2000 | 11093643 |
multiresidue method for the determination of residues of 251 pesticides in fruits and vegetables by gas chromatography/mass spectrometry and liquid chromatography with fluorescence detection. | a method is described for the determination of 251 pesticide and degradation product residues in fruit and vegetable samples. extraction of the sample with acetonitrile is followed by a salting-out step. co-extractives are removed by passing a portion of the acetonitrile extract through an octadecyl (c18) solid-phase extraction cleanup cartridge and then, in a second cleanup, through a carbon cartridge coupled to an amino propyl cartridge. determination is by gas chromatography with mass-selecti ... | 2008 | 10868594 |
food entrapped in papilla of vater: uncommon cause of vomiting. | a 20 month old girl was admitted for intractable vomiting over several days, with no other symptoms. family and personal history were not contributive. clinical and neurological examination, and routine blood tests and investigations (plain abdominal x ray, upper gastrointestinal tract contrast study, abdominal ultrasonography) were normal. the upper gastrointestinal endoscopy showed a mild antral gastritis and the second portion of duodenum was occupied by a tough, fibrous mass partially embedd ... | 2000 | 10833184 |
specific natural isotope profile studied by isotope ratio mass spectrometry (snip-irms): (13)c/(12)c ratios of fructose, glucose, and sucrose for improved detection of sugar addition to pineapple juices and concentrates. | the delta(13)c values of fructose, glucose, and sucrose have been determined in authentic pineapple juices. the sugar fraction is separated from the organic acids by an anionic exchange process. then the individual components (fructose, glucose, and sucrose) are isolated on a preparative hplc device using a nh(2)-type column. it is demonstrated that no significant isotope fractionation occurs when close to 100% of material is recovered and when the hydrolysis of sucrose is avoided. the control o ... | 1999 | 10794628 |
purification and characterization of macrodontain i, a cysteine peptidase from unripe fruits of pseudananas macrodontes (morr.) harms (bromeliaceae). | a new papain-like cysteine peptidase isolated from fruits of pseudananas macrodontes (morr.) harms, a species closely related to pineapple (ananas comosus l.), has been purified and characterized. the enzyme, named macrodontain i, is the main proteolytic component present in fruit extracts and was purified by acetone fractionation followed by anion-exchange chromatography. separation was improved by selecting both an adequate ph value and a narrow saline gradient. optimum ph range (more than 90% ... | 2000 | 10686143 |
routes of ethephon uptake in pineapple (ananas comosus) and reasons for failure of flower induction. | ethylene-releasing agents such as ethephon (2-chloroethylphosphonic acid) are used widely to induce flowering in pineapples (ananas comosus (l.) merrill). however, ethephon treatment is less reliable in summer, particularly if plants are treated on abnormally hot days. [(14)c]ethephon was used to follow uptake and translocation in leaf tissues. up to 30% of the ethephon entered the leaf within 4 h, and up to 60% by 24 h. uptake was dramatically modified by temperature, relative humidity, solutio ... | 1999 | 10688702 |
combinatorial approach to flavor analysis. 2. olfactory investigation of a library of s-methyl thioesters and sensory evaluation of selected components. | the odor characteristics of individual components present in a library comprised of s-methyl thioesters were determined independently by two laboratories using similar but not identical techniques. the odor potency was assessed by values of best estimate-gc-lower amount detected by sniffing (be-gc-loads). for small and medium chain s-methyl thioesters, these values were found to increase from 6 ng for s-methyl thiobutanoate to 90 ng for s-methyl thiostearate. all assessors detected a "green", "f ... | 1999 | 10552644 |
characterization of sinapyl derivatives in pineapple (ananas comosus [l.] merill) juice. | three previously unidentified phenolic compounds were found in pineapple (ananas comosus [l.] merill) juice in substantial concentrations and were isolated by semipreparative reverse phase hplc. the structures were elucidated from uv spectra, acid hydrolysis, and subsequent amino acid analysis, mass spectrometry, and two-dimensional nmr spectroscopy. the compounds are identified as s-sinapyl-l-cysteine, n-l-gamma-glutamyl-s-sinapyl-l-cysteine, and s-sinapylglutathione. | 1999 | 10552379 |
effects of oral bromelain administration on the impaired immunocytotoxicity of mononuclear cells from mammary tumor patients. | the protease bromelain from pineapple was suggested for adjuvant therapy of malignant diseases. we studied immunological effects of an orally applied bromelain drug on 16 breast cancer patients in comparison with healthy donors. bromelain was applied for 10 days with a daily dose of 3000 f.i.p. units and the immunocytotoxicity of blood monocytes and lymphocytes against the leukemic k562 and mda-mb-231 mammary carcinoma target cells was determined in vitro. in addition, the expression of the cell ... | 2006 | 10523679 |
role of carbohydrate moieties in ige binding to allergenic components of cupressus arizonica pollen extract. | a reduction of ige immunoreactivity after periodate-treatment has been previously reported for various glycoprotein allergens. | 1999 | 10457113 |
bromelain, from pineapple stems, proteolytically blocks activation of extracellular regulated kinase-2 in t cells. | recently, it has emerged that extracellular proteases have specific regulatory roles in modulating immune responses. proteases may act as signaling molecules to activate the raf-1/extracellular regulated kinase (erk)-2 pathway to participate in mitogenesis, apoptosis, and cytokine production. most reports on the role of protease-mediated cell signaling, however, focus on their stimulatory effects. in this study, we show for the first time that extracellular proteases may also block signal transd ... | 1999 | 10452995 |
incidence of patulin in fruits and fruit juices marketed in campinas, brazil. | one hundred and eleven samples of processed fruit juices (apple, grape, pineapple, papaya, guava, banana and mango) and 38 samples of sound fruits (apple, papaya, mango, pear and peach) produced and marketed in brazil, were analysed for patulin by hplc. only one out of 30 samples of apple juice was found positive at 17 micrograms/l. patulin was not detected in the other foodstuffs. it was found in 14 samples of spoiled fruit samples of apple (150-267 micrograms/kg), pear (134-245 micrograms/kg) ... | 1999 | 10435075 |
structural characterization of the n-linked oligosaccharides from tomato fruit. | the primary structures of the n-linked oligosaccharides from tomato fruit (lycopersicon esculentum) have been elucidated. for the isolation of the protein fraction, two procedures were employed alternatively: a low temperature acetone powder method and ammonium sulfate precipitation of the tomato extract. after peptic digestion, the glycopeptides were purified by cation-exchange chromatography; the oligosaccharides were released by n-glycosidase a and fluorescently labelled with 2-aminopyridine. ... | 1999 | 10365448 |
azoospermia and oligospermia among a large cohort of dbcp applicators in 12 countries. | azoospermia and oligospermia have been well demonstrated among workers exposed to 1,2-dibromo-3-chloropropane (dbcp) in manufacturing and formulation of this pesticide. after dbcp was banned in the united states in the late 1970s, two american companies continued to export it to many less developed countries. in the early to mid-1990s, attorneys assembled a cohort of approximately 26,400 male plaintiffs who, as workers on banana and pineapple plantations in 12 of these countries, had been expose ... | 2015 | 10330512 |
risk factors for latex allergy in patients with spina bifida and latex sensitization. | some subjects with spina bifida (sb) and latex sensitization (cutaneous and/or serum ige) can have clinical reactions, while others may have no symptoms after the exposure to latex products. | 1999 | 10231329 |
prevalence of and risk factors for latex sensitization in patients with spina bifida. | we determined the prevalence of and risk factors for latex sensitization in patients with spina bifida. | 1998 | 9783950 |
retrotransposon-like sequences integrated into the genome of pineapple, ananas comosus. | retrotransposon-like sequences have been serendipitously detected in the genome of commercial pineapple, ananas comosus. the sequence from a 2.6 kb cloned fragment of this element had greatest similarity to the del1 lilium henryi retrotransposon and the gypsy/ty3 group of retroelements. the order of the genes from 5' to 3' was reverse transcriptase, ribonuclease h and integrase. the integrase domain contained the amino acid sequence motifs which have been associated with recognition of the long ... | 1998 | 9747853 |
determination of soil-to-plant transfer factors of 137cs and 90sr in the tropical environment of bangladesh. | soil-to-plant transfer factors (tf) of 137cs and 90sr have been determined for different plants/crops, such as rice, beans, peanuts, pineapple, cabbage, tomato, spinach and grass. they were obtained from radioisotope experiments on plants grown in pots under outdoor ambient tropical conditions for three growing seasons (1994-1996). in the case of 137cs and concerning the above mentioned plants/crops, the average tfs were found to be 0.28, 0.25, 0.77, 0.19, 0.23, 0.28, 0.59 and 0.18, respectively ... | 1998 | 9728745 |
fear of negative evaluation and gender interact to predict alcoholic beverage preference. | this study was designed to look at the influence of gender, alcohol expectancies, and fear of negative evaluation on beverage selection. participants tasted pineapple juice, tonic water, beer, and diet cola and were then asked which they would prefer when presented with each of all possible alternative pairings. results indicated no effect on beer selection due to alcohol expectancies, but fear of negative evaluation and gender interacted to predict beer selection. post hoc analyses indicated th ... | 2004 | 9698979 |
the amino acid sequences of isoforms of the bromelain inhibitor from pineapple stem. | eight bromelain inhibitor (bi) isoform fractions were isolated from pineapple stem, and then submitted to conventional amino acid sequencing after performic acid oxidation and subsequent separation of the resulting light and heavy chains. the results revealed that all fractions exhibited microheterogeneity, containing at least two major components, but that all the isoinhibitors have a common double-chain structure (mr = ca. 5,700-5,900) with five disulfide bonds and similar amino acid sequences ... | 1998 | 9685742 |
[cross-reactivity between bromelain and soluble fraction from wheat flour]. | it is revealed that patients allergic to a water-soluble fraction from wheat flour were sensitive to pineapple enzyme, bromelain. since bromelain has a high similarity to other sh-proteases such as papain, it may imply that the patients may recognize varieties of other sh-proteases as the epitope. | 1997 | 9436335 |
pollination increases gibberellin levels in developing ovaries of seeded varieties of citrus. | reproductive and vegetative tissues of the seeded pineapple cultivars of sweet orange (citrus sinensis l.) contained the following c-13 hydroxylated gibberellins (gas): ga53, ga17, ga19, ga20, ga1, ga29, and ga8, as well as ga97, 3-epi-ga1, and several uncharacterized gas. the inclusion of 3-epi-ga1 as an endogenous substance was based on measurements of the isomerization rates of previously added [2h2]ga1. pollination enhanced amounts of ga19, ga20, ga29, and ga8 in developing ovaries. levels o ... | 1997 | 12223728 |
"latex-fruit syndrome": frequency of cross-reacting ige antibodies. | an association between allergies to latex proteins and to various foods has been reported and confirmed by rast and immunoblotting inhibition. however, no significant data had been collected on the frequency of specific ige antibodies to fruits in these patients and the frequency of a history of fruit intolerance. serum samples of 136 patients with well-documented, clinically relevant, immediate-type hypersensitivity against latex proteins were analyzed for ige antibodies against a panel of diff ... | 1997 | 9188921 |
purification and structural and kinetic characterization of the pyrophosphate:fructose-6-phosphate 1-phosphotransferase from the crassulacean acid metabolism plant, pineapple. | pyrphosphate-dependent phosphofructokinase (pfp) was purified to electrophoretic homogeneity from illuminated pineapple (ananas comosus) leaves. the purified enzyme consists of a single subunit of 61.5 kd that is immunologically related to the potato tuber pfp [beta] subunit. the native form of pfp likely consists of a homodimer of 97.2 kd, as determined by gel filtration. pfp's glycolytic activity was strongly dependent on ph, displaying a maximum at ph 7.7 to 7.9. gluconeogenic activity was re ... | 1997 | 12223643 |
[physico-chemical characterization of acerola (malpighia glabra l.) produced in maringá, paraná state, brazil]. | the acerola malpighia glabra l., originally from the antillas and north of south america, known by the people as cereja-das-antilhas or cereja-do-pará distinguish itself by its high content of vitamin c. the ripe and fresh acerola fruits utilized in experiments, were obtained from farmers of maringá region, paraná state, brazil. the fruits were hulled in steel sieve with 25 mesh and the bagasse (seeds and hull) discarded. these physico-chemical analysis were realized in the pulp: vitamin c, mois ... | 1997 | 9429646 |
determination of selenium levels in dairy products and drinks by hydride generation atomic absorption spectrometry: correlation with daily dietary intake. | the selenium concentrations in different dairy products and drinks in the mediterranean coastal area of southeastern spain have been determined by hydride generation atomic absorption spectrometry. mineralization was carried out with an hno3-hclo4 mixture (4:1) in a thermostatted sand bath. selenium determination was carried out by the standard addition method. mean concentrations obtained in diary products varied from 6.87 ng/g (rice pudding) to 178.90 ng/g (caramel custard), and those in drink ... | 2013 | 9102343 |
the prevalence of latex allergy in children seen in a university hospital allergy clinic. | natural rubber latex allergy is responsible for a wide spectrum of clinical symptoms, ranging from rhinoconjunctivitis to severe anaphylaxis, in both adults and children. an association between allergy to latex and allergy to various fruits has been reported. this study investigated the prevalence and clinical significance of latex sensitization in children seen in a university hospital allergy clinic. a total of 453 consecutive children were screened in a 7-month period. a detailed clinical his ... | 1997 | 9062637 |
coprecipitation of proteins with matrix ligands: scaleable protein isolation. | matrix ligands are agents for isolating proteins out of dilute crudes by coprecipitating proteins. the ligands have a strong anion sulfonate head which initiates binding to proteins having a positive net charge, zh+ approximately 5-20. initial binding tightens protein conformation and starts to squeeze water from conformationally motile proteins. the tails are stackable hydrophobic organic groups, azoaromatic dyes which draw protein-ligand complexes together. proteins coprecipitate as guests, in ... | 2013 | 9174921 |
synthesis and antifungal activity of cinnamic acid esters. | cinnamic, p-coumaric and ferulic acids were isolated from pineapple stems (ananas comosus var. cayenne). twenty-four kinds of esters were prepared from these acids, alcohols and the components of alpinia. isopropyl 4-hydroxycinnamate (11) and butyl 4-hydroxycinnamate (12) were found to have almost the same effectiveness in antifungal activity against pythium sp. at 10 ppm as that of the commercial fungicide iprobenfos (kitazin p). | 1996 | 8704323 |
solution structure of bromelain inhibitor iv from pineapple stem: structural similarity with bowman-birk trypsin/chymotrypsin inhibitor from soybean. | bromelain inhibitor vi from pineapple stem (bi-vi) is a unique double-chain inhibitor with an 11-residue light chain and a 41-residue heavy chain by disulfide bonds and inhibits the cysteine proteinase bromelain competitively. the structure of bi-vi in aqueous solution was determined using nuclear magnetic resonance spectroscopy and simulated annealing-based calculations. its three-dimensional structure was shown to be composed of two distinct domains, each of which is formed by a three-stranded ... | 1996 | 8611527 |
acidic catecholamine metabolites and 5-hydroxyindoleacetic acid in urine: the influence of diet. | concentrations of vanillylmandelic acid (vma), 3,4-dihydroxyphenylacetic acid (dopac), homovanillic acid (hva), vanillic acid (va) and 5-hydroxyindoleacetic acid (5-hiaa) in urine from healthy subjects were determined by a high-performance liquid chromatography system with a mixed-mode (c18/anion-exchange) column and an 8-channel electrochemical detector, in order to study the influence of diet, diurnal variation and age. the urinary excretion of 5-hiaa increased significantly after eating banan ... | 1996 | 8929065 |
neutral detergent fibre and pectic substances in pineapple and its derivates: juices and nectars. | | 1995 | 8549548 |
dietary fibre in pineapple fruit. | | 1995 | 8549542 |
[chemical characteristics of the pulp and oil of the annona tree (annona coriaceae)]. | belonging to the annonaceae family, marolo (annona coriaceae) is a native species of the brazilian "cerrado" región (minas gerais, goiás and distrito federal) and can be found in south american tropical zones. its fruits are highly consumed by local people and commercialized in markets or street stalls. there is, however, a tendency for the extinction of marolo due to deforestation and the large scale plantation of monocultures instead of native plants. the literature still offers no data on the ... | 1995 | 9382684 |
primary structure, sequence-specific 1h-nmr assignments and secondary structure in solution of bromelain inhibitor vi from pineapple stem. | one of the bromelain inhibitors, isoinhibitor vi (bi-vi), was purified from pineapple stem powder and its complete amino acid sequence was determined by conventional protein sequencing. these results revealed that the protein consists of an 11-residue light chain and a 41-residue heavy chain, cross-linked to each other by disulfide bonds to form the native inhibitor of 52 residues (m(r) = 5888). the secondary structure of bi-vi was analyzed based on the sequence-specific 1h resonance assignment ... | 1995 | 7556179 |
from the rotating drum to the pineapple-cancoil kidney: the unpublished history of the twin-coil. | | 1995 | 8573012 |
context effect of common objects on visual processing. | in previous experiments on visual processing it was shown that correct identification of sequentially and simultaneously presented numerals declined as the presentation interval was decreased from 50.0 to 16.7 ms/numeral. to determine how performance was influenced in context, common everyday objects were used in place of the numerals. these objects were grouped into 36 different categories with 4 objects per category. an example of a category was fruit: apple, banana, pear, pineapple. for all e ... | 1995 | 8539008 |
conformations and internal mobility of a glycopeptide derived from bromelain using molecular dynamics simulations and noesy analysis. | the conformation and internal flexibility of a glycopeptide manalpha1-6 (xylbeta1-2)manbeta1-4glcnacbeta1-4(fucalpha1-3) glcnacbeta1-n(asn-glu-ser-ser), prepared from pineapple stem bromelain, have been analyzed using a combination of molecular dynamics (md) simulations in water with noesy 1h nmr spectroscopy. theoretical noesy cross-peak intensities were calculated by the crosrel program on the basis of models, obtained from md simulations, using a full relaxation matrix approach. special atten ... | 1995 | 7663144 |
enhancement of ethylenethiourea recoveries in food analyses by addition of cysteine hydrochloride. | the effectiveness of cysteine hydrochloride (cys-hcl) as a preservative of ethylenethiourea (etu) in product matrixes and during analysis was studied. etu recoveries were adversely affected by certain product matrixes when fortified directly into the product. recoveries in 8 selected food items were 0-92% when analyzed 30 min after fortification and 0-51% when analyzed after 24 h. when cys-hcl was added to product prior to fortification, recoveries increased to 71-95% even after frozen storage f ... | 2013 | 7580323 |
the influence of flavor and color on drink identification by children and adults. | this study investigated how color and flavor influences drink identification by children and adults. the children ranged in age from 2 to 18 years of age. each subject tasted four drinks that differed in color and flavor. each drink had an atypical color-flavor pairing (e.g., brown-pineapple) or a typical pairing (e.g., brown-chocolate). after tasting each drink, the subject chose which of four flavor names identified the drink. for the atypical drinks, the selection of color-associated names (e ... | 1995 | 7621986 |
polysaccharide composition of unlignified cell walls of pineapple [ananas comosus (l.) merr.] fruit. | the polysaccharides of cell walls isolated from the fleshy, edible part of the fruit of the monocotyledon pineapple [ananas comosus (l.) merr.] (family bromeliaceae) were analyzed chemically. these cell walls were derived mostly from parenchyma cells and were shown histochemically to be unlignified, but they contained ester-linked ferulic acid. the analyses indicated that the noncellulosic polysaccharide composition of the cell walls was intermediate between that of unlignified cell walls of spe ... | 1995 | 7770529 |
[latex allergy in children: description of two cases]. | here we present two cases of latex hypersensitivity. the clinical manifestations were conjunctivitis, urticaria, angioedema and dermatitis. the patients presented positive skin prick test (spt) to latex with a commercial extract and by pricking through a latex surgical glove. radioallergosorbent test (rast) to latex and patch testing to common additives and to latex were negative. skin prick tests with fruits (banana, kiwi, pineapple, apricot, avocado, grape) were positive but children presented ... | 2006 | 7610085 |
isolation and partial characterization of basic proteinases from stem bromelain. | crude bromelain extracts from pineapple stems (ananas comosus) were fractionated by two-step fplc-cation-exchange chromatography. at least eight basic proteolytically active components were detected. the two main components f4 and f5 together with the most active proteinase fraction f9 were characterized by sds-page, mass spectroscopy, multizonal cathodal electrophoresis, partial amino acid sequence, and monosaccharide composition analysis. f9 amounts to about 2% of the total protein and has a 1 ... | 1995 | 7779262 |
effect of processing and storage on the ascorbic acid (vitamin c) content of some pineapple varieties grown in the rivers state of nigeria. | ascorbic acid contents of the juice of four different pineapples species grown in the rivers state of nigeria were determined before and after storage of whole pineapple and processing and storage of the juice for two months. ascorbic acid of the fresh juice ranged from 22.5 mg to 33.5 mg/100 g sample. after storage at room temperature (30-32 degrees c) of whole pineapple for two weeks, ascorbic acid was reduced to between 59 and 65 percent of the fresh juice. processing the juice by pasteurisat ... | 1994 | 7716115 |
measurement of 13co2 in expired air as an index of compliance to a high carbohydrate diet naturally enriched in 13c. | the aim of this study was to determine whether breath 13co2 measurements could be used to assess the compliance to a diet containing carbohydrates naturally enriched in 13c. the study was divided into two periods: period 1 (baseline of 4 days) with low 13c/12c ratio carbohydrates. period 2 (5 days) isocaloric diet with a high 13c/12c ratio (corn, cane sugar, pineapple, millet) carbohydrates. measurements were made of respiratory gas exchange by indirect calorimetry, urinary nitrogen excretion an ... | 1994 | 7812411 |
purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain. | a mixture of ananain (ec 3.4.22.31) and comosain purified from crude pineapple stem extract was found to contain numerous closely related enzyme forms. chromatographic separation of the major enzyme forms was achieved after treatment of the mixture with thiol-modifying reagents: reversible modification with 2-hydroxyethyl disulphide provided enzyme for kinetic studies, and irreversible alkylation with bromotrifluoroacetone or iodoacetamide gave enzyme for structural analyses by 19f-n.m.r. and el ... | 1994 | 8053898 |
normative data for commercial pineapple juice from concentrate. | normative data for pineapple juice from concentrate were determined for 19 samples, including 5 that had been aseptically processed and representing 4 of the major pineapple growing regions of the world. values are reported for sugars, organic acids, including isocitric acid, metals (specifically potassium, sodium calcium, and magnesium), delta 13c, and oligosaccharides. although geographical variation existed, the observed ranges and variances were small enough to be useful in describing authen ... | 2016 | 8069130 |
the precipitation of symptoms by common foods in children with atopic dermatitis. | atopic dermatitis (ad) is a chronic and disabling condition that has a major impact on financial and social resources of the individual and the community. its incidence is increasing dramatically, and no cure is available. pharmacological treatment is only partially effective. the evidence that diet plays a role in children with atopic dermatitis is now irrefutable. prophylactic measures can prevent or limit the development of ad, and partially restricted diets can modify the disease's course or ... | 2016 | 7806078 |
pineapple cysteine endopeptidases. | | 1994 | 7845232 |
sugarcane workers: morbidity and mortality. | sugarcane is, after pineapple, the largest agricultural industry in hawaii. there have been reports that this industry poses certain health hazards. to investigate this possible hazard in hawaii, the relationship of employment on a sugarcane plantation to total mortality, the development of definite coronary heart disease (chd), stroke, cancer, lung cancer and certain risk factors were examined in men of japanese ancestry participating in the honolulu heart program. after 18 years of follow-up, ... | 1993 | 8300403 |
a prospective randomized study with mineral oil and oral lavage solution for treatment of faecal impaction in children. | faecal impaction with or without encopresis is a very common problem. we performed a randomized, open-label, prospective study to compare the efficacy and acceptability of the treatment of faecal impaction using either mineral oil or pineapple-flavoured isotonic intestinal lavage solution containing polyethylene glycol-3350 (colyte). thirty-six patients over 2 years of age were randomized to receive either mineral oil or flavoured lavage solution. the dose of mineral oil was 2 to 8 tablespoons t ... | 1993 | 8280820 |
[effect of the addition of tropical fruits--pineapple (ananas comosus) and papaya (carica papaya)--on the production of biological silage from fish]. | microbial fish silage was produced from under-utilized fish mixed with juice and waste fruits (pineapple and papaya) at 35 degrees c. six different products were elaborated as following: a: fish muscle with pineapple juice; b: fish muscle with fruit wastes; c: gutted fish with pineapple juice; d: gutted fish with fruit wastes; e: whole fish with pineapple juice; f: whole fish with fruit wastes. process development was evaluated by measuring: ph, acidity, non-protein nitrogen, consistency and exu ... | 1993 | 8779625 |
chemical ecology of the palm weevilrhynchophorus palmarum (l.) (coleoptera: curculionidae): attraction to host plants and to a male-produced aggregation pheromone. | attraction to host plants by adultrhynchophorus palmarum (l.) palm weevils was studied in the field and in the laboratory. chemical analysis revealed the presence of ethanol and ethyl-acetate in stems of coco palms and in pineapple fruits and of pentane, hexanal, and isopentanol in coco stems. in the olfactometer, the first two compounds and isoamyl-acetate were attractive to the insects and the last three compounds, although not attractive by themselves, increased attractiveness when mixed with ... | 1993 | 24249235 |
systemic allergic reaction and diarrhoea after pineapple ingestion. | some foods may initiate allergic reactions. anaphylaxis due to mangoes, oranges, nuts and other foods has been reported earlier. we report the clinical and laboratory features of 32 patients who became symptomatic shortly after they had eaten pineapples. seventeen patients were males and 15 females with ages ranging from 5 to 70 years. most of the patients complained of intense itching and urticarial rashes, followed by abdominal pain, vomiting and diarrhoea. sixty-eight percent of the patients ... | 1993 | 8511816 |
purification and some properties of peroxidase isozymes from pineapple stem. | the enzyme peroxidase is widely distributed among the higher plants. isozymes of peroxidase are known to occur in a variety of tissues in a large number of plant species. in this study, peroxidase isozymes were purified from the extract of pineapple stem through successive steps of ammonium sulfate fractionation, cm-sepharose cl-6b chromatographies and deae-sepharose cl-6b chromatographies. by these steps, twelve isozymes of peroxidase were obtained. some properties of the isozymes were studied ... | 1993 | 8490564 |
influence of fruit and vegetable juices on the endogenous formation of n-nitrosoproline and n-nitrosothiazolidine-4-carboxylic acid in humans on controlled diets. | water, green pepper, pineapple, tomato, strawberry, carrot, and celery juices were made 46 mg/100 ml in ascorbic acid by the addition of distilled water or ascorbate. the ability of each juice to inhibit endogenous formation of n-nitrosoamino acids (naa) in humans was determined in controlled experiments. sixteen men consumed a standard diet low in nitrate and ascorbic acid for 18 consecutive days. nitrate (5.24 mmol) and l-proline (4.35 mmol) were given orally on days 3, 5, 7, 9, 11, 13, 15, 17 ... | 1992 | 1473234 |
characterization and structure of pineapple stem inhibitor of cysteine proteinases. | the complete amino acid sequence of the inhibitor of cysteine proteinases from pineapple stem acetone powder was determined. the inhibitor consists of 52 amino acids and is composed of two polypeptide chains (41 and 11 amino acids) linked via disulphide bonds. it differs from already known sequences in one to four amino acids. data from its amino acid sequence analysis clearly show that this inhibitor cannot be a member of the cystatin superfamily. the ki values for papain, bromelain and catheps ... | 1992 | 1515075 |
asean. | asean is a group of counties encompassing 3 million sq. km with 333 million inhabitants, of whom approximately 65% are literate. the group was formed august 8, 1967, and includes brunei, indonesia, malaysia, the philippines, singapore, and thailand. malay, indonesian, filipino, thai, chinese, and english are spoken by malay, thai, chinese, indian, and other ethnic groups who variously are of the islamic, buddhist, christian, taoist, and hindu faiths. life expectancy is approximately 85 years, wi ... | 1992 | 12178041 |
diversifying crops: the nicaraguan experiment. | over 1/2 of nicaragua's population lives in the pacific plains where cotton has been grown intensively for 40 years. this single-crop economy has led to massive deforestation, wind and water erosion has affected the soil, and extensive use of pesticides has deposited excessive amounts of ddt in the breast milk of nursing mothers. after the downfall of the somoza dictatorship the subsequent agrarian reform has been hampered by lack of information and training on sustainable methods of farming. th ... | 1992 | 12317702 |
effect of fabrication, sterilization and mediators--blood compatibility of polyurethanes. | the possible changes in the surface and physical properties of polyether urethane urea (peuu) implants, including their interaction with blood, due to the different preparation methods, sterilization techniques and long term storage in different environmental conditions have been investigated by conducting the studies of mechanical properties, contact angle, platelet adhesion and protein adsorption. considerable variations in the mechanical properties have been observed for the peuu grafts store ... | 1992 | 1573556 |
[obtainment of pineapple juice powder by foam-mat drying]. | the foam-mat production and stability using pineapple juice concentrate (25, 30 and 40 degrees brix), adding a surfactants mixture and maltodextrin (de 10) as co-adjuvant, stirred in a commercial mixer, was studied. adequate foam formation conditions were as follows: concentrate of 25 degrees brix using surface active agents (sorbac 60-polisorbac 80) 0.285% surface active agent/total solids, hlb = 6, and stirring time, 7 min. the foam was dehydrated in an oven dried with a horizontal air flow ci ... | 1991 | 1811453 |
atomic absorption spectrophotometric method for determination of polydimethylsiloxane residues in pineapple juice: collaborative study. | an atomic absorption spectrophotometric method for determination of polydimethylsiloxane (pdms) residues in pineapple juice was collaboratively studied by 9 laboratories. pdms residues are extracted from pineapple juice with 4-methyl-2-pentanone and the extracted silicone is measured by atomic absorption spectrophotometry using a nitrous oxide/acetylene flame. collaborators analyzed 5 samples including 1 blind duplicate. reproducibility relative standard deviations (rsdr) were 13.1% at 31 ppm, 6 ... | 2003 | 2272995 |
on the pharmacology of bromelain: an update with special regard to animal studies on dose-dependent effects. | bromelain, a standardized complex of proteases from the pineapple plant, is absorbed unchanged from the intestine of animals at a rate of 40%; in animal experiments it was found to have primarily anti-edema, antiinflammatory, and coagulation-inhibiting effects. these effects are due to an enhancement of the serum fibrinolytic activity and inhibition of the fibrinogen synthesis, as well as a direct degradation of fibrin and fibrinogen. bromelain lowers kininogen and bradykinin serum and tissue le ... | 1990 | 2203073 |
morphology of griseofulvin-resistant isolates of mongolian variant of trichophyton schoenleini. | clinical, experimental and electronmicroscopic observations, and computer-enhanced images were made on 5 patients with tinea favosa resistant to griseofulvin. the pathologic fungus was identified as the mongolian variant of trichophyton schoenleini. the results of physical examinations, routine tests of blood, urine and stool, liver and immunologic function examinations were normal in all of the 5 patients. under scanning electron microscopy, the surface of the spore showed pineapple-like form, ... | 1990 | 2119962 |
determination of total sulfite in shrimp, potatoes, dried pineapple, and white wine by flow injection analysis: collaborative study. | a method for the determination of total sulfite in shrimp, potatoes, dried pineapple, and white wine by flow injection analysis (fia) was collaboratively studied by 8 laboratories. in the method, the sample solution is reacted with sodium hydroxide to liberate aldehyde-bound sulfite. the sample stream is acidified to produce so2 gas, which diffuses across a teflon membrane in the gas diffusion cell into a flowing stream of malachite green. the degree of discoloration of the malachite green is pr ... | 2013 | 2312511 |
[food taboos in the northern region of brazil]. | a study was carried out into alimentary taboos in two localities (são félix and murumuru) in the county of marabá, state of pará, brazil. ninety families were studied in são félix and thirty-five in murumuru. as regards the feeding taboos found, the most frequent was that prohibiting the simultaneous ingestion of milk and various types of fruit, including especially mango, orange, cashew and pineapple. the ingestion of eggs with fruit was also considered harmful as well as the eating of mammalia ... | 1989 | 2641837 |
[antiphlogistic effect of bromelaine following third molar removal]. | a placebo-controlled double-blind study of 100 patients with impacted and/or dislocated lower wisdom teeth was conducted to examine the tolerance and antiphlogistic efficacy of bromelaine--a mixture of proteolytic enzymes from ananas comosus. treatment was started 1 day prior to third molar surgery with a daily dose of 3 x 80 mg and was continued for a total of 6 days. on the 1st day following surgery, linear measurement (distance: tragus-pogonion) showed swelling to be 7.5% lower under drug tre ... | 1989 | 2700702 |
soluble sugars as the carbohydrate reserve for cam in pineapple leaves : implications for the role of pyrophosphate:6-phosphofructokinase in glycolysis. | neutral ethanol-soluble sugar pools serve as carbohydrate reserves for crassulacean acid metabolism (cam) in pineapple (ananas comosus (l.) merr.) leaves. levels of neutral soluble sugars and glucans fluctuated reciprocally with concentrations of malic acid. hexose loss from neutral soluble-sugar pools was sufficient to account for malic acid accumulation with about 95% of the required hexose accounted for by turnover of fructose and glucose pools. hexose loss from starch or starch plus lower mo ... | 1989 | 16666775 |
co(2) and o(2) exchanges in the cam plant ananas comosus (l.) merr: determination of total and malate-decorboxylation-dependent co(2)-assimilation rates; study of light o(2)-uptake. | photosynthesis and light o(2)-uptake of the aerial portion of the cam plant ananas comosus (l.) merr. were studied by co(2) and o(2) gas exchange measurements. the amount of co(2) which was fixed during a complete day-night cycle was equal to the amount of total net o(2) evolved. this finding justifies the assumption that in each time interval of the light period, the difference between the rates of net o(2)-evolution and of net light atmospheric co(2)-uptake give the rates of malate-decarboxyla ... | 1989 | 16666547 |
[comparative observations of the behavior of ethephon in fruit, coffee and paprika]. | after application of flordimex to soft and stone fruit for fruit abscission and acceleration of ripening obvious ethephon residues have been found. therefore, the dose rate has to be restricted to keep the maximum residue limit. the same is true for the use of flordimex for fruit abscission in apples, whereas for yield regulation residues are far below 0.05 mg/kg in apples. a typical feature of the active ingredient is the large quantity of its residues in juice and wine, which is as high as eth ... | 1989 | 2608103 |
production of pineapple plants in vitro. | in vitro culture of pineapple (ananas comosus) was studied to determine the efficiency of axillary bud culture for rapid propagation of several cultivars. the technique used maximizes the success rate of various steps in the production of pineapple plants. rapid mass multiplication of plantlets started 9 months after explanting with a significant log phase. the number of plantlets obtained from the culture of a single bud by the thirteenth month ranged from 210 to 380 for 'perolera'; 300 to 350 ... | 1988 | 24240410 |
a carbohydrate epitope expressed uniquely on the cell surface of drosophila neurons is altered in the mutant nac (neurally altered carbohydrate). | antibodies against horseradish peroxidase (anti-hrp) recognize neural specific cell surface antigens in drosophila and other insects. the nature of these antigens was investigated in drosophila and found to include a complex set of developmentally regulated proteins. their common epitope appears to be a carbohydrate that shares features with the sugar moiety of pineapple stem bromelain, a plant glycoprotein whose carbohydrate structure has been determined. a mutation was identified that eliminat ... | 1988 | 2463162 |
bromelain, the enzyme complex of pineapple (ananas comosus) and its clinical application. an update. | after a short description of the uses of pineapple as folk medicine by the natives of the tropics, the more important new pharmaceutical applications of bromelain, reported between 1975 and 1978, are presented. although the exact chemical structure of all active components of bromelain is not fully determined, this substance has shown distinct pharmacological promise. its properties include: (1) interference with growth of malignant cells; (2) inhibition of platelet aggregation; (3) fibrinolytic ... | 2005 | 3287010 |
antimetastatic effect of bromelain with or without its proteolytic and anticoagulant activity. | bromelain, a pineapple-derived plant product, added to c57bl/6 mice laboratory chow decreased lung metastasis of lewis lung cancer cells implanted s.c. this antimetastatic potential was demonstrated by both the active and inactive bromelain with or without proteolytic, anticoagulant properties. | 1988 | 3182910 |
milk free desserts. | robinson's baby foods have added a new range of milk free desserts, including banana and pineapple treat and summer fruit salad. the desserts can be mixed with water for mothers who need to feed their babies on a milk free diet. | 1987 | 27319541 |
occupational exposure to 1,3-dichloropropene (telone ii) in hawaiian pineapple culture. | 1,3-dichloropropene (dcp), the primary constituent of telone ii, is a subsoil fumigant that has supplanted 1,2-dibromo-3-chloropropane (dbcp) and ethylene dibromide (edb) as primary fumigant in hawaiian pineapple culture. to determine the potential for adverse health effects, an environmental survey was done to assess worker exposures. exposures were predominantly below 1 ppm, which is the no-effect level determined with experimental animals, and the threshold limit value (tlv) recommended by th ... | 2016 | 3452296 |
fructose 2,6-bisphosphate, carbohydrate partitioning, and crassulacean acid metabolism. | fructose 2,6-bisphosphate (f 2,6-p(2)) was detected in the cam species, ananas comosus and bryophyllum tubiflorum, and in c(3)- and cam-mesembryanthemum crystallinum. in both mesembryanthemum tissues, f 2,6-p(2) was located outside the chloroplast. the levels of f 2,6-p(2), malate, starch, or soluble sugars were measured during various periods during the day-night cycle in the leaves of ananas, a species which stores carbohydrate in an extrachloroplastic compartment, and in bryophyllum, a specie ... | 1987 | 16665395 |
survey of occupational injuries on a large pineapple plantation during the harvesting season. | | 1987 | 3570760 |
sulfur dioxide effects on plants exhibiting crassulacean acid metabolism. | the effects of so(2) on species exhibiting crassulacean acid metabolism (cam) were determined with short term-high concentration 'acute' greenhouse exposures (0.6 to 3.0 microl liter(-1) (ppm) so(2) for 2 and 8 h), and long term-low concentration 'chronic' field exposures (0.35 to 0.90 microl liter(-1) so(2) for 32 to 79 h periodically over 7 to 13 days). in the acute greenhouse exposures, visible injury was observed on opuntia basilaris engelm. & bigel., exposed to 2.0 microl liter(-1) so(2), b ... | 1987 | 15092814 |
electrophoretic analysis of protoplast, vacuole, and tonoplast vesicle proteins in crassulacean acid metabolism plants. | protoplasts and vacuoles were isolated and purified in large numbers from the cam plants ananas comosus (pineapple) and sedum telephium for protein characterization. vacuoles were further fractionated to yield a tonoplast vesicle preparation. polypeptides of protoplasts, vacuoles, and tonoplast vesicles were compared to whole leaf polypeptides from both plants by one-dimensional sodium dodecylsulfate-polyacrylamide gel electrophoresis. approximately 100 vacuole polypeptides could be resolved of ... | 1986 | 16665166 |
inhibition of tumour growth in vitro by bromelain, an extract of the pineapple plant (ananas comosus). | | 1985 | 17345291 |
inhibition of tumour growth in vitro by bromelain, an extract of the pineapple plant (ananas comosus). | | 1985 | 4095199 |