| end-products, fermentation balances and molar growth yields of homofermentative lactobacilli. | | 1972 | 4630544 |
| effect of alcohol on serum folate level. | alcohol was given orally and intravenously to normal and chronic alcoholic volunteers to study its effect on folate metabolism. oral alcohol, given to nine subjects on a low folate diet, caused a greater fall in serum lactobacillus casei folate levels than that seen in eight subjects on a low folate diet alone. this alcohol-induced fall in serum folate level occurred largely during the 1st day of the protocol. although brief infusions of intravenous ethanol had no effect on serum folate level, a ... | 1973 | 4630980 |
| ingestion and absorption of naturally occurring pteroylmonoglutamates (folates) in man. | | 1973 | 4631311 |
| [mechanisms of bacteriostasis and bactericidal activity in the stomach and upper small intestine]. | | 1972 | 4632020 |
| occurrence of glucosamine residues with free amino groups in cell wall peptidoglycan from bacilli as a factor responsible for resistance to lysozyme. | analysis by dinitrophenylation techniques revealed the occurrence of significant amounts of glucosamine residues with free amino groups in the peptidoglycan component of cell walls isolated from bacillus cereus, bacillus subtilis, and bacillus megaterium. a close correlation was demonstrated between the content of n-unacetylated glucosamine residues in the peptidoglycan component and the resistance of the cell walls to lysozyme. these lysozyme-resistant cell walls and peptidoglycan were converte ... | 1973 | 4632317 |
| experience with microbiological assay for folate using a chloramphenicol-resistant l. casei strain. | experience with a semi-automated l. casei folate assay using a chloramphenicol-resistant strain of organism is described. control of ph of medium and incubation time was required. the normal range for serum folate was 2 to 16 ng/ml and red cell folate 200 to 600 ng/ml. | 1972 | 4632329 |
| trimethoprim-sulphamethoxazole and folate metabolism. | | 1973 | 4632643 |
| a comparison of serum folate estimations using two different methods. | | 1973 | 4632762 |
| a qualitative platelet defect in severe vitamin b 12 deficiency. response, hyperresponse, and thrombosis after vitamin b 12 therapy. | | 1973 | 4632789 |
| spiral plate method for bacterial determination. | a method is described for determining the number of bacteria in a solution by the use of a machine which deposits a known volume of sample on a rotating agar plate in an ever decreasing amount in the form of an archimedes spiral. after the sample is incubated, different colony densities are apparent on the surface of the plate. a modified counting grid is described which relates area of the plate of volume of sample. by counting an appropriate area of the plate, the number of bacteria in the sam ... | 1973 | 4632851 |
| biosynthesis of membrane teichoic acid. a role of the d-alanine-activating enzyme. | | 1973 | 4633673 |
| ecology of human oral lactobacilli. | lactobacilli were found in saliva, on teeth, and on the dorsum of the tongue, the vestibular mucosa, and the hard palate in humans. their proportions in saliva, expressed as percentage of the anaerobically cultivable flora, were 10- to 100-fold higher than those on the tooth surface, but were comparable to those on the epithelial surfaces. the adherence of lactobacillus casei and l. fermenti to oral surfaces was compared with that of streptomycin-labeled streptococcus sanguis and s. salivarius b ... | 1972 | 4637297 |
| the formation of -cyclohexyl-fatty acids from shikimate in an acidophilic thermophilic bacillus. a new biosynthetic pathway. | labelled acetate, phenylalanine and shikimic acid were fed to bacillus acidocaldarius. a high proportion of the (14)c incorporated from acetate and shikimate was recovered in methyl esters from the cell lipids, but such recovery of (14)c from phenylalanine was low. only the (14)c from shikimate was selectively incorporated into 11-cyclohexylundecanoate and 13-cyclohexyltridecanoate. degradation of these cyclohexyl-fatty acids showed that shikimate was incorporated as an intact c(7) unit. | 1972 | 4638790 |
| study of antibacterial activity and bacteriology of latex from asclepias syriaca l. | whole and fractionated latex of asclepias syriaca was tested for antimicrobial or growth-promoting activity with 16 genera and species of bacteria. latex and extracted fractions (distilled water, acetic acid, sodium bicarbonate, sulfuric acid, and ethyl ether) possessed no detectable antimicrobial activity. comparison of growth curves of selected bacteria incubated with serum and rubber fractions, as well as controls, revealed no detectable inhibition of growth, except for a slight inhibitory re ... | 1973 | 4790590 |
| synthesis of substituted 4-dimethylaminoazobenzenes and a study of their effect on lactobacillus casei and escherichia coli. | | 1968 | 4872194 |
| anomalous anaerobic growth yields of lactobacillus casei strain 103 in complex medium. | | 1968 | 4873000 |
| growth-physiological changes in escherichia coli and other bacteria during division inhibition by 5-diazouracil. | diazouracil (dzu) was shown to increase the mean cell volume of several bacterial species. the filaments which it induced with escherichia coli b and lactobacillus casei were examined in section by electron microscopy. in addition to the inhibition of division of e. coli by dzu, its effects were studied on mass increase, viability, and deoxyribonucleic acid, ribonucleic acid, and protein synthesis. the dependence of cell age on the division-inhibitory effect of dzu was examined in synchronous cu ... | 1969 | 4884820 |
| relationship of spermidine and nucleic acids to growth in lactobacillus casei and escherichia coli. | | 1969 | 4890256 |
| guanine and xanthine phosphoribosyltransfer activities of lactobacillus casei and escherichia coli. their relationship to hypoxanthine and adenine phosphoribosyltransfer activities. | | 1970 | 4910918 |
| properties of thymidylate synthetase from dichloromethotrexate-resistant lactobacillus casei. | | 1970 | 4918842 |
| molar growth yields and fermentation balances of lactobacillus casei l3 in batch cultures and in continuous cultures. | | 1970 | 4930427 |
| diacetyl and acetoin production by lactobacillus casei. | agitation of broth cultures of lactobacillus casei retarded cellular dry weight accumulation but enhanced production of both diacetyl and acetoin. addition of pyruvate overcame this retardation, but addition of sulfhydryl-protecting reagents did not. both pyruvate and citrate enhanced accumulated dry weight of l. casei incubated without agitation, but only pyruvate increased diacetyl accumulation. both actively dividing cells and cells suspended in buffer converted pyruvate to diacetyl and aceto ... | 1971 | 4943268 |
| a study of folate absorption and metabolism in man utilizing carbon-14--labeled polyglutamates synthesized by the solid phase method. | the absorption and metabolism of synthetic polyglutamates of folic acid have been compared with free pteroylglutamic acid in four subjects having chronic lymphatic leukemia and one with hodgkin's granuloma. pteroylpolyglutamates containing either three or seven glutamate residues were prepared by the solid-phase method permitting placement of carbon-14 labels in either the pteridine ring or in a selected glutamate unit of the gamma peptide chain. complete dissociation was observed between biolog ... | 1969 | 4977032 |
| dextran production by a human oral strain of lactobacillus casei. | | 1969 | 4979949 |
| studies of (3h)folic acid uptake by lactobacillus casei. | | 1970 | 4985961 |
| bacteriophage active against the lactic acid beverage-producing bacterium lactobacillus casei. | a virulent bacteriophage which causes a decrease in acid production during fermentation of a lactic acid beverage named yakult with lactobacillus casei was isolated from the abnormal fermentation tank and named pl-1. l. casei s strain was the exclusive host cell among 18 lactic acid bacteria tested. the plaque was round with an average diameter of about 0.5 mm. it exhibited serological cross-reaction with previously isolated j1 phage. under an electron microscope, the phage had a spermatozoon sh ... | 1970 | 4991958 |
| comparative studies of fructose 1,6-diphosphate aldolase from escherichia coli 518 and lactobacillus casei var. rhamnosus atcc 7469. | | 1971 | 4994351 |
| intracellular polysaccharide production by human oral strains of lactobacillus casei. | | 1971 | 4995779 |
| studies on the absorption and metabolism of folic acid. i. folate absorption in the dog after exposure of isolated intestinal segments to synthetic pteroylpolyglutamates of various chain lengths. | folic acid absorption was studied in anesthetized dogs by determining the amount and chemical nature of folate in venous blood emerging from isolated intestinal segments containing free folic acid and/or pteroylpolyglutamates of a known chain length. chromatographically pure test materials placed in the lumen were prepared by unambiguous solid phase synthetic methods. this synthetic procedure not only yields compounds of known structure, it also provides a means by which glutamic acid residues a ... | 1971 | 5000560 |
| detection of relationships between streptococcus faecalis and lactobacillus casei by immunological studies with two forms of malic enzyme. | antisera prepared against two isologous malic enzymes from lactobacillus casei strains 64h and m40 were used to survey and categorize the various malic enzymes found within this diverse species. in addition to detecting three major antigenic variants of malic enzyme within this group, both antisera readily reacted with streptococcus faecalis malic enzyme. the cross-reactions between the l. casei malic enzyme antisera and the s. faecalis malic enzyme indicated that these iso-functional enzymes fo ... | 1971 | 5001196 |
| the bactoprenol content of plasma and mesosome membranes from lactobacillus casei. | | 1971 | 5001951 |
| thymidylate synthetase and its relationship to dihydrofolate reductase. | | 1971 | 5002295 |
| confirmation of collateal sensitivity to pyrimethamine in lactobacillus casei atcc 7469 resistant to chlorguanide triazine. | | 1971 | 5002297 |
| discussion paper: amethopterin linked covalently to water-soluble macromolecules. | | 1971 | 5002437 |
| action of diastereoisomers of tetrahydrohomofolate on the growth of lactobacillus casei. | | 1971 | 5002438 |
| folate antagonists as antibacterial and antiprotozoal agents. | | 1971 | 5002439 |
| pteroylglutamate derivatives in pisum sativum l. biosynthesis of cotyledonary tetrahydropteroylglutamates during germination. | 1. the concentrations of pteroylglutamate derivatives in the cotyledons of pea (pisum sativum l.) seedlings were determined by microbiological assay by using lactobacillus casei (a.t.c.c. 7469), streptococcus faecalis (a.t.c.c. 8043) and pediococcus cerevisiae (a.t.c.c. 8081). during germination the pteroylglutamate content of the cotyledons increased rapidly from 0.2mug/g dry wt. to 4.0mug/g dry wt., the maximum values being reached approx. 120h after imbibition. 2. approx. 50% of the pteroylgl ... | 1971 | 5003530 |
| [the sensitivity of microorganisms to growth factors. ii. effect of various reducing agents on the extraction and determination of folates by lactobacillus casei, streptococcus faecalis and pediococcus cerevisiae]. | | 1971 | 5003561 |
| crystalline thymidylate synthetase from dichloromethotrexate resistant lactobacillus casei. | | 1971 | 5003635 |
| 2,4-diamino-6-arylethylpteridines as streptococcus faecium growth inhibitors. | | 1971 | 5003787 |
| specific establishment of lactobacilli in the digestive tract of germ-free chickens. | | 1971 | 5003790 |
| a lytic enzyme(s) acting on lactobacilli. | | 1971 | 5003791 |
| experimental granulomatous myocarditis: genesis and immunological aspects. | | 1969 | 5003876 |
| free and conjugated folates in the livers of kittens. | | 1971 | 5005208 |
| laboratory aids in the diagnosis of folic acid and vitamin b 12 deficiencies. | | 1971 | 5005357 |
| [the detection of folic acid deficiency by determination of l. casei folate activity in blood serum, total blood, and red cells]. | | 1971 | 5005658 |
| [the ability of milk fermentation bacteria to use vitamin b 12 - 58 co]. | | 1971 | 5005659 |
| [results of comparative riboflavin analysis in the comecon countries]. | | 1971 | 5006002 |
| lactobacillus casei tablets in the treatment of intestinal infection. | | 1971 | 5135364 |
| studies on encapsulated lactobacilli. iv. sucrose utilization by lactobacillus casei. | | 1966 | 5226799 |
| further characterization of an antibacterial factor in human parotid secretions, active against lactobacillus casei. | | 1965 | 5226826 |
| dental caries in gnotobiotic rats inoculated with lactobacillus casei. | | 1968 | 5240782 |
| in vitro demonstration of a vasodepressant enhanced by lactobacillus casei. | | 1967 | 5248981 |
| studies on the regulation of one-carbon metabolism. the effects of folate concentration in the growth medium on the activity of three folate-dependent enzymes in lactobacillus casei. | | 1969 | 5253268 |
| the temperate phages of lactobacillus salivarius and lactobacillus casei. | | 1970 | 5311549 |
| phage receptor material in lactobacillus casei cell wall. i. effect of l-rhamnose on phage adsorption to the cell wall. | | 1971 | 5316574 |
| toxic properties of the cell wall of gram-positive bacteria. | the biological activity of odontomyces viscosus, which has been reported to cause periodontal disease in hamsters, was examined. the microorganism was cultured anaerobically in brain heart infusion broth, and the cells were harvested. the washed cells were injected intradermally into the abdomen of rabbits. after 72 hr, a well-defined, firm, raised nodule (about 1.0 by 1.5 cm) with an erythematous border was seen at the injection site. suspensions of cell wall and cytoplasmic material were injec ... | 1967 | 5335900 |
| structure of the cell wall peptidoglycan of lactobacillus casei ro94. | | 1969 | 5367451 |
| the measurement of serum pyridoxal by a microbiological assay using lactobacillus casei. | a new method has been developed for the assay of serum pyridoxal using l. casei. bound pyridoxal phosphate in serum is converted by acid hydrolysis to pyridoxal for which this organism is specific. this method proved to be considerably more sensitive than other methods so far reported in the literature. serum pyridoxal concentrations were measured in 151 control subjects aged 17 to 80 years. the range of concentrations found was 1.5 to 13.5 ng/ml which compared well with values obtained by most ... | 1970 | 5420735 |
| [studies on the antibacterial action against cariogenic lactobacillus casei of the salivary peroxidase antibacterial system]. | | 1970 | 5422777 |
| the inhibitory effect of tungstate on the assay of riboflavin by lactobacillus casei (atcc 7469) and its reversal by molybdate. | | 1970 | 5427034 |
| growth stimulation of lactobacillus casei by sodium citrate. | | 1970 | 5441913 |
| diacetyl reductase of lactobacillus casei. | | 1970 | 5483647 |
| identification of a stimulant for lactobacillus casei produced by streptococcus lactis. | a compound stimulatory to the growth of lactobacillus casei was isolated from cell extracts of streptococcus lactis, purified, and characterized. the stimulant was identified as a small peptide with a molecular weight of approximately 4,500 daltons. the purified peptide gave negative tests for nucleic acids, phosphorus, glucosamine, and carbohydrates. sixteen amino acids were detected in acid hydrolysates of this peptide. serine, proline, glycine, alanine, leucine, and glutamic acid were present ... | 1970 | 5485084 |
| characteristics of growth inhibition of lactobacillus casei by 4-nitroquinoline-n-oxide. | the bacteriostatic action of 4-nitroquinoline-n-oxide (4-nqo) for lactobacillus casei is substantially reversed by d-and l-cysteine, glutathione, and 2,2-dihydroxy-1,4-dithiolbutane (dithioerythritol). the action appears to involve a chemical reaction between carbon atom 4 of 4-nqo and nucleophilic centers, such as -sh groups, located on essential cell constituents. the evidence presented indicates that the protective effect of d- and l-cysteine, glutathione, and dithioerythritol against the act ... | 1970 | 5498599 |
| inhibition of growth of lactobacillus casei by tungstate and its reversal by molybdate. | | 1970 | 5500289 |
| thymidylate synthetase from amethopterin-resistant lactobacillus casei. | | 1971 | 5538615 |
| conversion of vitamin b 6 compounds to active forms in the red blood cell. | in studies with pyridoxine and other b(6) compounds in blood, the active forms pyridoxal and pyridoxal phosphate were measured by differential assays using lactobacillus casei. red cell uptake of tritiated pyridoxine was also measured. a new metabolic pathway for conversion of pyridoxine to active forms was demonstrated in red cells. in vivo studies in normal subjects suggested that pyridoxine was taken up by red cells where it was converted to pyridoxal phosphate and then pyridoxal, followed by ... | 1971 | 5567559 |
| adaptive alterations in the fermentative sequence of lactobacillus casei. | | 1968 | 5650312 |
| the glycolipids of lactobacillus casei a.t.c.c. 7469. | 1. the lipids were extracted from lactobacillus casei a.t.c.c. 7469 with chloroform-methanol mixtures. the glycolipids were obtained by chromatography on silicic acid and deae-cellulose (acetate form). 2. hydrolysis of the glycolipids with alkali gave two glycerol glycosides and a mixture of fatty acids. 3. the glycosides were separated and their structures elucidated. the major component was o-alpha-d-galactopyranosyl-(1-->2)-o-alpha-d-glucopyranosyl-(1-->1)-glycerol and the minor component o-a ... | 1968 | 5660629 |
| ultrastructure and sites of tetranito-blue tetrazolium reduction of lactobacillus casei. | | 1968 | 5667334 |
| effect of light on the lactobacillus casei icrobiological assay. | because of the considerable differences in the reported values for serum folate in normal subjects using lactobacillus casei as assay organism the effect of daylight when preparing media for this assay was studied. it was found that the growth properties of the medium were destroyed by direct sunlight and impaired by bright reflected sunlight, therefore reducing the sensitivity of the assay. adequate precautions regarding exposure to light must be taken. | 1968 | 5697339 |
| [on inhibition of the growth of lactobacillus casei by some antiphlogistic agents]. | | 1968 | 5711868 |
| estimation of red cell folate activity. | the conditions described by hoffbrand, newcombe, and mollin (1966) for the estimation of red cell folate activity have been confirmed using a different method (spray, 1964) of micro-biological assay with lactobacillus casei. in 81 control subjects the levels of folate activity in the red cells were between 80 and 470 mmug/ml (mean 192), and in plasma between 2.1 and 13 mmug/ml (mean 4.9). in 16 patients with megaloblastic anaemia due to folic acid deficiency, the red cell folate level was betwee ... | 1969 | 5776553 |
| substitution of oxytetracycline for riboflavin as a growth factor for lactobacillus casei, strain epsilon. | | 1969 | 5817428 |
| the linkage between the polysaccharide and mucopeptide components of the cell wall of lactobacillus casei. | 1. the linkage between the polysaccharide and mucopeptide components of the cell wall of lactobacillus casei is rapidly hydrolysed under mild acid-hydrolysis conditions. 2. the release of the polysaccharide is accompanied by the hydrolysis of an n-acetylhexosaminide linkage. the n-acetylhexosamine residue readily forms chromogen and it is concluded that it is substituted on c((3)) by the adjacent sugar. 3. continued heating of the polysaccharide in acid results in a slower release of reactive n- ... | 1965 | 5837777 |
| properties of the polysaccharide and mucopeptide components of the cell wall of lactobacillus casei. | 1. the polysaccharide and mucopeptide components of the cell wall of lactobacillus casei have been separated by mild conditions of acid hydrolysis. 2. removal of the polysaccharide renders the mucopeptide susceptible to lysozyme. 3. the mucopeptide and polysaccharide components have been analysed and the results compared with those obtained previously. 4. the polysaccharides responsible for group specificity have a terminal reducing n-acetylgalactosamine residue substituted on c((3)) by the adja ... | 1965 | 5837778 |
| numerical survey of some bacterial taxa. | focht, d. d. (iowa state university, ames), and w. r. lockhart. numerical survey of some bacterial taxa. j. bacteriol. 90:1314-1319. 1965.-a numerical analysis was made of 77 properties of each of 43 bacterial strains, representing 25 genera from 8 families in the orders eubacteriales and pseudomonadales. four major groups were found, related to one another at approximately the same level of similarity: (1) a large cluster containing the subgroups (1a) athiorhodaceae-spirillaceae, (1b) xanthomon ... | 1965 | 5848329 |
| technicon autoanalysis of the ninhydrin-positive phospholipids of lactobacillus casei. | | 1965 | 5866156 |
| phage susceptibility of encapsulated lactobacillus casei. | | 1965 | 5866164 |
| aseptic addition method for lactobacillus casei assay of folate activity in human serum. | an ;aseptic addition' method is described for microbiological assay with lactobacillis casei of folate activity in human serum. it has the following advantages over the previously reported ;standard' method. 1 the manipulations involved in the assay are halved, by deleting autoclaving of serum in buffers. 2 the use of 1 g.% ascorbate better preserves serum folates than the lower amounts of ascorbate which are the maximum quantities usable in the standard methods. 3 only 0.3 ml. of serum is requi ... | 1966 | 5904975 |
| method of assay of red cell folate activity and the value of the assay as a test for folate deficiency. | a simplified microbiological assay for determining the folate content of red cells is described. as in previously reported methods lactobacillus casei is used as test organism but two modifications are introduced. first, haemolysis is carried out in water containing 1 g.% of ascorbic acid; secondly, haemolysates are not incubated before the assay. using this assay, recovery of pteroylglutamic acid added in two different concentrations to five different whole blood samples was 97.0 +/- 1.9 s.e. % ... | 1966 | 5904976 |
| effect of ascorbic acid on the serum folic acid estimation. | ascorbic acid added to the basal medium increased the growth response of lactobacillus casei in folic acid standards. the effect in the serum extracts was not marked, resulting in lowered serum folic acid estimations. however, results obtained on prolonged incubation were similar whether ascorbic acid was added or not. the presence or absence of ascorbic acid and variation of the incubation period may account for differences in reported normal ranges of serum folic acid levels. | 1966 | 5904981 |
| resistance to inhibitors of dihydrofolate reductase in strains of lactobacillus casei and proteus vulgaris. | | 1966 | 5912585 |
| modifications in the lactobacillus casei assay of serum folate activity. | | 1966 | 5916020 |
| serum lactobacillus casei activity: ascorbic acid and the "thermo-labile factor". | | 1966 | 5919100 |
| modifications in the lactobacillus casei assay of serum folate activity. | | 1966 | 5921416 |
| metabolism of biotin and analogues of biotin by microorganisms. iv. degradation of biotin, oxybiotin, and desthiobiotin by lactobacillus casei. | birnbaum, jerome (university of cincinnati, cincinnati, ohio), and herman c. lichstein. metabolism of biotin and analogues of biotin by microorganisms. iv. degradation of biotin, oxybiotin, and desthiobiotin by lactobacillus casei. j. bacteriol. 92:925-930. 1966.-lactobacillus casei degrades biotin when it is present in excess to products not utilizable for growth by l. plantarum or saccharomyces cerevisiae. degrading activity was initiated in the early stationary phase and was controlled by the ... | 1966 | 5926760 |
| [the determination of substances with folic acid activity in serum with lactobacillus casei in the diagnosis of folic acid deficiency]. | | 1966 | 5935414 |
| complete remission in two cases of choriocarcinoma with observations of uric acid metabolism and serum inhibition of lactobacillus casei. | | 1966 | 5940876 |
| growth response of lactobacillus casei (atcc 7469) to riboflavin, fmn, and fad. | | 1966 | 5944861 |
| the utilization of riboflavin antagonists by a mutant of lactobacillus casei. | | 1966 | 5971734 |
| [a lactic acid mixture from lactobacillus casei in the diet of infants]. | | 1966 | 5986610 |
| lactobacillus casei c.s.f. folate activity. | | 1967 | 6036432 |
| effect of antimicrobial agents on the lactobacillus casei folate assay. | | 1967 | 6053928 |
| futile xylitol cycle in lactobacillus casei. | a futile xylitol cycle appears to be responsible for xylitol-mediated inhibition of growth of lactobacillus casei cl-16 at the expense of ribitol. the gratuitously induced xylitol-specific phosphoenolpyruvate-dependent phosphotransferase accumulates the pentitol as xylitol-5-phosphate, a phosphatase cleaves the latter, and an export system expels the xylitol. operation of the cycle rapidly dissipates the ribitol-5-phosphate pool (and ultimately the energy supply of the cell), thereby producing b ... | 1984 | 6090413 |
| oxidation of thymidylate synthase by inorganic compounds. | thymidylate synthase from methotrexate-resistant lactobacillus casei was rapidly and completely inactivated by low concentrations of permanganate, periodate, or potassium triiodide at 0 degree c. the enzyme was not inactivated to any appreciable extent by iodate, iodide, ferricyanate, iodosobenzoate, or hydrogen peroxide. the inactivation by permanganate was retarded by the substrate 2'-deoxyuridylate and, to a lesser extent, by phosphate. titration of enzyme activity with permanganate showed th ... | 1984 | 6094726 |
| the inactivation of thymidylate synthase by periodate. | thymidylate synthase from methotrexate-resistant lactobacillus casei rapidly lost about 90% of its catalytic activity when incubated with an equimolar concentration of io4- at 0 degree c. nearly complete inhibition resulted when the io4- concentration was twice the enzyme concentration or higher. the inhibition reaction appeared to be pseudo-first-order with respect to enzyme when io4- was in excess. the substrate dump, the product dtmp, and inorganic phosphate all protected the enzyme from inac ... | 1984 | 6097648 |
| deficient autolytic enzyme activity in antibiotic-tolerant lactobacilli. | to define the mechanism(s) of penicillin tolerance in lactobacilli, one nontolerant and two tolerant strains were examined for autolytic enzyme activity. when incubated with 14c-labeled cell wall preparations, autolysin extracts of tolerant lactobacilli released significantly less radioactivity than did extracts of nontolerant lactobacilli (p less than 0.02). these differences in the release of radioactivity by nontolerant and tolerant strains were maximal during the logarithmic growth phase. mo ... | 1982 | 6123485 |
| purification and properties of lactobacillus casei folylpoly-gamma-glutamate synthetase. | folylpolyglutamate synthetase was purified 200,000-fold from extracts of lactobacillus casei. the homogeneous protein was a monomer of mr = 43,000. the purified enzyme catalyzed a mgatp-dependent addition of glutamate to 5,10-methylene-tetrahydropteroylmono-, di-, and triglutamate substrates and metabolized (6r)-5,10-methylene-tetrahydro[3h]folate to the tetraglutamate derivative. other folate derivatives were poor substrates or lacked activity. the specificity of the nucleotide site was wide. t ... | 1983 | 6138353 |