| lineage and virulence of streptococcus suis serotype 2 isolates from north america. | we performed multilocus sequence typing of 64 north american streptococcus suis serotype 2 porcine isolates. strains were sequence type (st) 28 (51%), st25 (44%), and st1 (5%). we identified nonrandom associations between sts and expression of the virulence markers suilysin (sly), muramidase-relased protein (mrp), and extracellular factor (ef). expression of pili encoded by the srtf and srtg pilus clusters was also nonrandomly associated with sts. st1 strains were sly+ ef+ mrp+ srtf pilus+ srtg ... | 2011 | 22172538 |
| Application of a bacteriophage lysin to disrupt biofilms formed by the animal pathogen Streptococcus suis. | Bacterial biofilms are crucial to the pathogenesis of many important infections and are difficult to eradicate. Streptococcus suis is an important pathogen of pigs, and here the biofilm-forming ability of 32 strains of this species was determined. Significant biofilms were completely formed by 10 of the strains after 60 h of incubation, with exopolysaccharide production in the biofilm significantly higher than that in the corresponding planktonic cultures. S. suis strain SS2-4 formed a dense bio ... | 2011 | 21984241 |
| identification of a novel streptococcal adhesin p (sadp) protein recognizing galactosyl-α1-4-galactose-containing glycoconjugates: convergent evolution of bacterial pathogens to binding of the same host receptor. | bacterial adhesion is often a prerequisite for infection, and host cell surface carbohydrates play a major role as adhesion receptors. streptococci are a leading cause of infectious diseases. however, only few carbohydrate-specific streptococcal adhesins are known. streptococcus suis is an important pig pathogen and a zoonotic agent causing meningitis in pigs and humans. in this study, we have identified an adhesin that mediates the binding of s. suis to galactosyl-α1-4-galactose (galα1-4gal)-co ... | 2011 | 21908601 |
| genetic analysis of the capsular polysaccharide synthesis locus in 15 streptococcus suis serotypes. | the capsular polysaccharide (cps) synthesis locus of 13 streptococcus suis serotypes (serotype 1, 3, 4, 5, 7, 8, 9, 10, 14, 19, 23, 25 and 1/2) was sequenced and compared with that of serotype 2 and 16. the cps synthesis locus of these 15 serotypes falls into two genetic groups. the locus is located on the chromosome between orfz and aroa. all the translated proteins in the cps synthesis locus were clustered into 127 homology groups using the tribemcl algorithm. the general organization of the l ... | 2011 | 22092812 |
| streptococcus suis, an emerging drug-resistant animal and human pathogen. | streptococcus suis, a major porcine pathogen, has been receiving growing attention not only for its role in severe and increasingly reported infections in humans, but also for its involvement in drug resistance. recent studies and the analysis of sequenced genomes have been providing important insights into the s. suis resistome, and have resulted in the identification of resistance determinants for tetracyclines, macrolides, aminoglycosides, chloramphenicol, antifolate drugs, streptothricin, an ... | 2011 | 22275909 |
| optimized protocol for multiplex nested polymerase chain reaction to detect and differentiate haemophilus parasuis, streptococcus suis, and mycoplasma hyorhinis in formalin-fixed, paraffin-embedded tissues from pigs with polyserositis. | an optimized protocol was developed for the simultaneous detection and differentiation of haemophilus parasuis, streptococcus suis, and mycoplasma hyorhinis in formalin-fixed, paraffin-embedded (ffpe) tissues with multiplex nested polymerase chain reaction (pcr). this method also determines the prevalence of these bacteria in pigs with polyserositis. dna extraction with a combination of a commercial reagent and proteinase k resulted in more frequent detection of the pathogens than dna extraction ... | 2012 | 23277698 |
| catabolite control protein a of streptococcus suis type 2 contributes to sugar metabolism and virulence. | catabolite control protein a (ccpa) is the major transcriptional regulator in carbon catabolite repression in several gram-positive bacteria. we attempted to characterize the role of a ccpa homologue of streptococcus suis type 2 in sugar metabolism and virulence. addition of glucose or sucrose to the defined medium significantly reduced the activity of raffinose-inducible α-galactosidase, cellobiose-inducible β-glucosidase, and maltose-inducible α-glucosidase of the wild-type strain by about 9, ... | 2012 | 23274986 |
| postmortem diagnosis of sudden unexpected death from streptococcus suis type 2 infection: a case report. | streptococcus suis causes meningitis, septicemia, arthritis, endocarditis and death in both pigs and humans. sudden death is rarely documented in the forensic field and almost all cases were diagnosed pre-mortem. here we report a 49-year-old man who died from s. suis type 2 infection. s. suis was identified as the causative pathogen using bacterial culture, standard biochemical and coagglutination tests, specific dna amplification by polymerase chain reaction, and histopathologic examination. we ... | 2012 | 23622489 |
| structure determination of streptococcus suis serotype 14 capsular polysaccharide. | the capsular polysaccharide (cps) of streptococcus suis serotype 14 was purified, chemically modified, and characterized. sugar and absolute configuration analyses gave the following cps composition: d-gal, 3; d-glc, 1; d-glcnac, 1; d-neu5ac, 1. the sambucus nigra lectin, which recognizes the neu5ac(α2-6)gal/galnac sequence, showed binding to the native cps. sialic acid was found to be terminal, and the cps was quantitatively desialylated by mild acid hydrolysis. it was also submitted to perioda ... | 2012 | 23527632 |
| fhb, a novel factor h-binding surface protein, contributes to the antiphagocytic ability and virulence of streptococcus suis. | streptococcus suis serotype 2 is a gram-positive bacterium that causes sepsis and meningitis in piglets and humans. the mechanisms of s. suis serotype 2 invasive disease are not well understood. the surface proteins of pathogens usually play important roles in infection and bacterium-host interactions. here, we identified a novel surface protein that contributed significantly to the virulence of s. suis serotype 2 in a piglet infection model. this protein showed little similarity to other report ... | 2012 | 22526676 |
| antibody response specific to the capsular polysaccharide is impaired in streptococcus suis serotype 2-infected animals. | streptococcus suis serotype 2 is an extracellular encapsulated bacterium that causes severe septicemia and meningitis in swine and humans. albeit crucial in the fight against encapsulated bacteria, the nature of the capsular polysaccharide (cps)-specific antibody (ab) response during s. suis type 2 infection is unknown. we compared for the first time the features of cps-specific versus protein-specific ab responses during experimental infections with live virulent s. suis type 2 in mice. the pri ... | 2014 | 25385801 |
| identification and characterization of the chromosomal yefm-yoeb toxin-antitoxin system of streptococcus suis. | toxin-antitoxin (ta) systems are widely prevalent in the genomes of bacteria and archaea. these modules have been identified in escherichia coli and various other bacteria. however, their presence in the genome of streptococcus suis, an important zoonotic pathogen, has received little attention. in this study, we describe the identification and characterization of a type ii ta system, comprising the chromosomal yefm-yoeb locus of s. suis. the yefm-yoeb locus is present in the genome of most sero ... | 2015 | 26272287 |
| cultural drivers and health-seeking behaviours that impact on the transmission of pig-associated zoonoses in lao people's democratic republic. | pig rearing is an important income source in the lao people's democratic republic (pdr), with many smallholder farmers using traditional free-range pig production systems. despite the potentially significant health risks posed by pig production regarding pig-associated zoonoses, information on the sociocultural drivers of these zoonoses is significantly lacking. this review summarises the existing sociocultural knowledge on eight pig-associated zoonoses suspected to be endemic in southeast asia: ... | 2015 | 25973203 |
| attenuation of streptococcus suis virulence by the alteration of bacterial surface architecture. | neub, a sialic acid synthase catalyzes the last committed step of the de novo biosynthetic pathway of sialic acid, a major element of bacterial surface structure. here we report a functional neub homologue of streptococcus suis, a zoonotic agent, and systematically address its molecular and immunological role in bacterial virulence. disruption of neub led to thinner capsules and more susceptibility to ph, and cps2b inactivation resulted in complete absence of capsular polysaccharides. these two ... | 2012 | 23050094 |
| effect of spatial separation of pigs on spread of streptococcus suis serotype 9. | the spread of an infectious agent in a population can be reduced by interfering in the infectiousness or susceptibility of individuals, and/or in their contact structure. the aim of this study was to quantify the effect of prevention of direct contact between infectious and susceptible pigs on the transmission of streptococcus suis (s. suis). in three replicate experiments, s. suis-free pigs were housed in boxes either in pairs (25 pairs) or alone (15 pigs). the distance between the boxes was ±1 ... | 2013 | 23593467 |
| bacterial etiologies of five core syndromes: laboratory-based syndromic surveillance conducted in guangxi, china. | under the existing national surveillance system in china for selected infectious diseases, bacterial cultures are performed for only a small percentage of reported cases. we set up a laboratory-based syndromic surveillance system to elucidate bacterial etiologic spectrum and detect infection by rare etiologies (or serogroups) for five core syndromes in the given study area. | 2014 | 25360596 |
| suicin 90-1330 from a nonvirulent strain of streptococcus suis: a nisin-related lantibiotic active on gram-positive swine pathogens. | streptococcus suis serotype 2 is known to cause severe infections (meningitis, endocarditis, and septicemia) in pigs and is considered an emerging zoonotic agent. antibiotics have long been used in the swine industry for disease treatment/prevention and growth promoters. this pattern of utilization resulted in the spread of antibiotic resistance in s. suis worldwide. interestingly, pigs may harbor s. suis in their tonsils without developing diseases, while north american strains belonging to the ... | 2014 | 24973067 |
| crystal structure and identification of two key amino acids involved in ai-2 production and biofilm formation in streptococcus suis luxs. | streptococcus suis has emerged as an important zoonotic pathogen that causes meningitis, arthritis, septicemia and even sudden death in pigs and humans. quorum sensing is the signaling network for cell-to-cell communication that bacterial cells can use to monitor their own population density through production and exchange of signal molecules. s-ribosylhomocysteinase (luxs) is the key enzyme involved in the activated methyl cycle. autoinducer 2 (ai-2) is the adduct of borate and a ribose derivat ... | 2015 | 26484864 |
| suicin 3908, a new lantibiotic produced by a strain of streptococcus suis serotype 2 isolated from a healthy carrier pig. | while streptococcus suis serotype 2 is known to cause severe infections in pigs, it can also be isolated from the tonsils of healthy animals that do not develop infections. we hypothesized that s. suis strains in healthy carrier pigs may have the ability to produce bacteriocins, which may contribute to preventing infections by pathogenic s. suis strains. two of ten s. suis serotype 2 strains isolated from healthy carrier pigs exhibited antibacterial activity against pathogenic s. suis isolates. ... | 2015 | 25659110 |
| clearance of streptococcus suis in stomach contents of differently fed growing pigs. | streptococcus (s.) suis translocates across the intestinal barrier of piglets after intraintestinal application. based on these findings, an oro-gastrointestinal infection route has been proposed. thus, the objective of this study was to investigate the survival of s. suis in the porcine stomach. whereas surviving bacteria of s. suis serotypes 2 and 9 were not detectable after 60 min of incubation in stomach contents with a comparatively high gastric ph of 5 due to feeding of fine pellets, the n ... | 2016 | 27509526 |
| comparative proteomic analysis of streptococcus suis biofilms and planktonic cells that identified biofilm infection-related immunogenic proteins. | streptococcus suis (ss) is a zoonotic pathogen that causes severe disease symptoms in pigs and humans. biofilms of ss bind to extracellular matrix proteins in both endothelial and epithelial cells and cause persistent infections. in this study, the differences in the protein expression profiles of ss grown either as planktonic cells or biofilms were identified using comparative proteomic analysis. the results revealed the existence of 13 proteins of varying amounts, among which six were upregula ... | 2012 | 22514606 |
| functional definition of bira suggests a biotin utilization pathway in the zoonotic pathogen streptococcus suis. | biotin protein ligase is universal in three domains of life. the paradigm version of bpl is the escherichia coli bira that is also a repressor for the biotin biosynthesis pathway. streptococcus suis, a leading bacterial agent for swine diseases, seems to be an increasingly-important opportunistic human pathogen. unlike the scenario in e. coli, s. suis lacks the de novo biotin biosynthesis pathway. in contrast, it retains a bioy, a biotin transporter-encoding gene, indicating an alternative survi ... | 2016 | 27217336 |
| sub-mics of azithromycin decrease biofilm formation of streptococcus suis and increase capsular polysaccharide content of s. suis. | streptococcus suis (s. suis) caused serious disease symptoms in humans and pigs. s. suis is able to form thick biofilms and this increases the difficulty of treatment. after growth with 1/2 minimal inhibitory concentration (mic) of azithromycin, 1/4 mic of azithromycin, or 1/8 mic of azithromycin, biofilm formation of s. suis dose-dependently decreased in the present study. furthermore, scanning electron microscopy analysis revealed the obvious effect of azithromycin against biofilm formation of ... | 2016 | 27812354 |
| complete genome sequence, lifestyle, and multi-drug resistance of the human pathogen corynebacterium resistens dsm 45100 isolated from blood samples of a leukemia patient. | corynebacterium resistens was initially recovered from human infections and recognized as a new coryneform species that is highly resistant to antimicrobial agents. bacteremia associated with this organism in immunocompromised patients was rapidly fatal as standard minocycline therapies failed. c. resistens dsm 45100 was isolated from a blood culture of samples taken from a patient with acute myelocytic leukemia. the complete genome sequence of c. resistens dsm 45100 was determined by pyrosequen ... | 2012 | 22524407 |
| gida, a trna modification enzyme, contributes to the growth, and virulence of streptococcus suis serotype 2. | glucose-inhibited division protein (gida), is a trna modification enzyme functioning together with mnme in the addition of a carboxymethylaminomethyl group to position 5 of the anticodon wobble uridine of trna. here, we report a gida homolog from a chinese isolate sc-19 of the zoonotic streptococcus suis serotype 2 (ss2). gida disruption led to a defective growth, increased capsule thickness, and reduced hemolytic activity. moreover, the gida deletion mutant (δgida) displayed reduced mortality a ... | 2016 | 27148493 |
| rapid visual detection of highly pathogenic streptococcus suis serotype 2 isolates by use of loop-mediated isothermal amplification. | streptococcus suis serotype 2 (s. suis 2) is an important zoonotic pathogen that causes considerable economic losses to the pig industry and significantly threatens public health worldwide. the highly pathogenic s. suis 2, which contains the 89k pathogenicity island (pai), has caused large-scale outbreaks of infections in humans, resulting in high mortality rates. in this study, we established two loop-mediated isothermal amplification (lamp)-based assays that can rapidly detect s. suis 2 and th ... | 2013 | 23884995 |
| trem-1 signaling promotes host defense during the early stage of infection with highly pathogenic streptococcus suis. | infection with highly pathogenic streptococcus suis can cause septic shock, which is characterized by high levels of inflammatory cytokines and a high mortality rate. our previous study indicated that trem-1 (triggering receptor expressed on myeloid cells 1) was upregulated in swine spleen cells in response to s. suis infection. the role of trem-1 signaling in enhancement of the proinflammatory response promoted us to examine its effect on the outcome of s. suis infection. in the present study, ... | 2015 | 26056380 |
| auxotrophic actinobacillus pleurpneumoniae grows in multispecies biofilms without the need for nicotinamide-adenine dinucleotide (nad) supplementation. | actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, which causes important worldwide economic losses in the swine industry. several respiratory tract infections are associated with biofilm formation, and a. pleuropneumoniae has the ability to form biofilms in vitro. biofilms are structured communities of bacterial cells enclosed in a self-produced polymer matrix that are attached to an abiotic or biotic surface. virtually all bacteria can grow as a biofi ... | 2016 | 27349384 |
| a case of streptococcus suis infection causing pneumonia with empyema in korea. | streptococcus suis causes meningitis and sepsis in pigs, but human infection has increased over the past few years in those who are exposed to pigs or raw pork. most cases have occurred in southeast asia, but only two cases have been reported in south korea, presenting with arthritis and meningitis. here, we report a rare case of s. suis infection, a 60-year-old sailor, who visited the emergency room presenting septicemia, pneumonia with empyema and meningitis, showed full recovery; however, neu ... | 2012 | 23166552 |
| isolation and characterization of a native avirulent strain of streptococcus suis serotype 2: a perspective for vaccine development. | streptococcus suis, an emerging infectious pathogen, is the cause of two large-scale outbreaks of human streptococcal toxic shock syndrome in china, and has attracted much attention from the scientific community. the genetic basis of its pathogenesis remains enigmatic, and no effective prevention measures have been established. to better understand the virulence differentiation of s. suis and develop a promising vaccine, we isolated and sequenced a native avirulent s. suis strain (05has68). anim ... | 2015 | 25891917 |
| egfr transactivation contributes to neuroinflammation in streptococcus suis meningitis. | streptococcus suis serotype 2 (ss2) is an important zoonotic bacterial pathogen in both humans and animals, which can cause high morbidity and mortality. meningitis is one of the major clinical manifestations of ss2 infection. however, the specific process of ss2 meningitis and its molecular mechanisms remain unclear. epidermal growth factor receptor (egfr) has been reported to initiate transduction of intracellular signals and regulate host inflammatory responses. whether and how egfr contribut ... | 2016 | 27756321 |
| recruitment of factor h to the streptococcus suis cell surface is multifactorial. | streptococcus suis is an important bacterial swine pathogen and a zoonotic agent. recently, two surface proteins of s. suis, fhb and fhbp, have been described for their capacity to bind factor h-a soluble complement regulatory protein that protects host cells from complement-mediated damages. results obtained in this study showed an important role of host factor h in the adhesion of s. suis to epithelial and endothelial cells. both fhb and fhbp play, to a certain extent, a role in such increased ... | 2016 | 27399785 |
| aetiologies of central nervous system infection in viet nam: a prospective provincial hospital-based descriptive surveillance study. | infectious diseases of the central nervous system (cns) remain common and life-threatening, especially in developing countries. knowledge of the aetiological agents responsible for these infections is essential to guide empiric therapy and develop a rational public health policy. to date most data has come from patients admitted to tertiary referral hospitals in asia and there is limited aetiological data at the provincial hospital level where most patients are seen. | 2012 | 22662232 |
| polar invasion and translocation of neisseria meningitidis and streptococcus suis in a novel human model of the blood-cerebrospinal fluid barrier. | acute bacterial meningitis is a life-threatening disease in humans. discussed as entry sites for pathogens into the brain are the blood-brain and the blood-cerebrospinal fluid barrier (bcsfb). although human brain microvascular endothelial cells (hbmec) constitute a well established human in vitro model for the blood-brain barrier, until now no reliable human system presenting the bcsfb has been developed. here, we describe for the first time a functional human bcsfb model based on human choroid ... | 2012 | 22253884 |
| capsular sialic acid of streptococcus suis serotype 2 binds to swine influenza virus and enhances bacterial interactions with virus-infected tracheal epithelial cells. | streptococcus suis serotype 2 is an important swine bacterial pathogen, and it is also an emerging zoonotic agent. it is unknown how s. suis virulent strains, which are usually found in low quantities in pig tonsils, manage to cross the first host defense lines to initiate systemic disease. influenza virus produces a contagious infection in pigs which is frequently complicated by bacterial coinfections, leading to significant economic impacts. in this study, the effect of a preceding swine influ ... | 2013 | 24082069 |
| group b streptococcus and streptococcus suis capsular polysaccharides induce chemokine production by dendritic cells via toll-like receptor 2- and myd88-dependent and -independent pathways. | streptococcus agalactiae (also known as group b streptococcus [gbs]) and streptococcus suis are encapsulated streptococci causing severe septicemia and meningitis. bacterial capsular polysaccharides (cpss) are poorly immunogenic, but anti-cps antibodies are essential to the host defense against encapsulated bacteria. the mechanisms underlying anti-cps antibody responses are not fully elucidated, but the biochemistry of cpss, particularly the presence of sialic acid, may have an immunosuppressive ... | 2013 | 23774593 |
| minimum core genome sequence typing of bacterial pathogens: a unified approach for clinical and public health microbiology. | bacterial pathogens impose a heavy health burden worldwide. in the new era of high-throughput sequencing and online bioinformatics, real-time genome typing of infecting agents, and in particular those with potential severe clinical outcomes, holds promise for guiding clinical care to limit the detrimental effects of infections and to prevent potential local or global outbreaks. here, we sequenced and compared 85 isolates of streptococcus suis, a zoonotic human and swine pathogen, wherein we anal ... | 2013 | 23720795 |
| bacterial adhesion of streptococcus suis to host cells and its inhibition by carbohydrate ligands. | streptococcus suis is a gram-positive bacterium, which causes sepsis and meningitis in pigs and humans. this review examines the role of known s. suis virulence factors in adhesion and s. suis carbohydrate-based adhesion mechanisms, as well as the inhibition of s. suis adhesion by anti-adhesion compounds in in vitro assays. carbohydrate-binding specificities of s. suis have been identified, and these studies have shown that many strains recognize galα1-4gal-containing oligosaccharides present in ... | 2013 | 24833053 |
| epidemiology, clinical manifestations, and outcomes of streptococcus suis infection in humans. | streptococcus suis, a bacterium that affects pigs, is a neglected pathogen that causes systemic disease in humans. we conducted a systematic review and meta-analysis to summarize global estimates of the epidemiology, clinical characteristics, and outcomes of this zoonosis. we searched main literature databases for all studies through december 2012 using the search term "streptococcus suis." the prevalence of s. suis infection is highest in asia; the primary risk factors are occupational exposure ... | 2014 | 24959701 |
| role of capsule and suilysin in mucosal infection of complement-deficient mice with streptococcus suis. | virulent streptococcus suis serotype 2 strains are invasive extracellular bacteria causing septicemia and meningitis in piglets and humans. one objective of this study was to elucidate the function of complement in innate immune defense against s. suis. experimental infection of wild-type (wt) and c3(-/-) mice demonstrated for the first time that the complement system protects naive mice against invasive mucosal s. suis infection. s. suis wt but not an unencapsulated mutant caused mortality asso ... | 2014 | 24686060 |
| streptococcus suis meningitis: a systematic review and meta-analysis. | streptococcus suis is the most common cause of meningitis in pork consuming and pig rearing countries in south-east asia. we performed a systematic review of studies on s. suis meningitis to define the clinical characteristics, predisposing factors and outcome. | 2015 | 26505485 |
| interactions of streptococcus suis serotype 2 with human meningeal cells and astrocytes. | streptococcus suis serotype 2 is an important porcine pathogen and emerging zoonotic agent responsible for meningitis, of which different sequence types predominate worldwide. though bacterial meningitis is defined as an exacerbated inflammation of the meninges, the underlying astrocytes of the glia limitans superficialis may also be implicated. however, the interactions between this pathogen and human meningeal cells or astrocytes remain unknown. furthermore, the roles of well-described virulen ... | 2015 | 26502903 |
| isolation, characterization and biological properties of membrane vesicles produced by the swine pathogen streptococcus suis. | streptococcus suis, more particularly serotype 2, is a major swine pathogen and an emerging zoonotic agent worldwide that mainly causes meningitis, septicemia, endocarditis, and pneumonia. although several potential virulence factors produced by s. suis have been identified in the last decade, the pathogenesis of s. suis infections is still not fully understood. in the present study, we showed that s. suis produces membrane vesicles (mvs) that range in diameter from 13 to 130 nm and that appear ... | 2015 | 26110524 |
| immune-responsiveness of cd4(+) t cells during streptococcus suis serotype 2 infection. | the pathogenesis of streptococcus suis infection, a major swine and human pathogen, is only partially understood and knowledge on the host adaptive immune response is critically scarce. yet, s. suis virulence factors, particularly its capsular polysaccharide (cps), enable this bacterium to modulate dendritic cell (dc) functions and potentially impair the immune response. this study aimed to evaluate modulation of t cell activation during s. suis infection and the role of dcs in this response. s. ... | 2016 | 27905502 |
| identification of streptococcus suis meningitis through population-based surveillance, togo, 2010-2014. | during 2010-2014, we enrolled 511 patients with suspected bacterial meningitis into surveillance in 2 districts of northern togo. we identified 15 persons with streptococcus suis infection; 10 had occupational contact with pigs, and 12 suffered neurologic sequelae. s. suis testing should be considered in rural areas of the african meningitis belt. | 2016 | 27314251 |
| protection against streptococcus suis serotype 2 infection using a capsular polysaccharide glycoconjugate vaccine. | streptococcus suis serotype 2 is an encapsulated bacterium and one of the most important bacterial pathogens in the porcine industry. despite decades of research for an efficient vaccine, none is currently available. based on the success achieved with other encapsulated pathogens, a glycoconjugate vaccine strategy was selected to elicit opsonizing anti-capsular polysaccharide (anti-cps) igg antibodies. in this work, glycoconjugate prototypes were prepared by coupling s. suis type 2 cps to tetanu ... | 2016 | 27113360 |
| binding of human fibrinogen to mrp enhances streptococcus suis survival in host blood in a αxβ2 integrin-dependent manner. | the gram-positive bacterium streptococcus suis serotype 2 (s. suis 2), an important zoonotic pathogen, induces strong systemic infections in humans; sepsis and meningitis are the most common clinical manifestations and are often accompanied by bacteremia. however, the mechanisms of s. suis 2 survival in human blood are not well understood. in our previous study, we identified muramidase-released protein (mrp), a novel human fibrinogen (hfg)-binding protein (fbp) in s. suis 2 that is an important ... | 2016 | 27231021 |
| host-pathogen interactions in bacterial meningitis. | bacterial meningitis is a devastating disease occurring worldwide with up to half of the survivors left with permanent neurological sequelae. due to intrinsic properties of the meningeal pathogens and the host responses they induce, infection can cause relatively specific lesions and clinical syndromes that result from interference with the function of the affected nervous system tissue. pathogenesis is based on complex host-pathogen interactions, some of which are specific for certain bacteria, ... | 2016 | 26744349 |
| functional and structural characterization of the antiphagocytic properties of a novel transglutaminase from streptococcus suis. | streptococcus suis serotype 2 (ss2) is an important swine and human zoonotic pathogen. in the present study, we identified a novel secreted immunogenic protein, sstgase, containing a highly conserved eukaryotic-like transglutaminase (tgase) domain at the n terminus. we found that inactivation of sstgase significantly reduced the virulence of ss2 in a pig infection model and impaired its antiphagocytosis in human blood. we further solved the crystal structure of the n-terminal portion of the prot ... | 2015 | 26085092 |
| simultaneous quantification and differentiation of streptococcus suis serotypes 2 and 9 by quantitative real-time pcr, evaluated in tonsillar and nasal samples of pigs. | invasive streptococcus suis (s. suis) infections in pigs are often associated with serotypes 2 and 9. mucosal sites of healthy pigs can be colonized with these serotypes, often multiple serotypes per pig. to unravel the contribution of these serotypes in pathogenesis and epidemiology, simultaneous quantification of serotypes is needed. a quantitative real-time pcr (qpcr) targeting cps2j (serotypes 2 and 1/2) and cps9h (serotype 9) was evaluated with nasal and tonsillar samples from s. suis expos ... | 2016 | 27376336 |
| occurrence of pasteurella multocida among pigs with respiratory disease in china between 2011 and 2015. | prior to the 1990s, p. multocida capsular serogroup a was the most prevalent in china, followed by serogroups b and d. thirty years later, serogroup d became the most prevalent, followed by serogroups a and b. however, the p. multocida capsular serogroups currently circulating in china remain unclear. therefore, the aim of the present study was to provide an update on p. multocida serogroups isolated from diagnostic samples collected from clinically diseased pigs in central and eastern china fro ... | 2017 | 28078081 |
| comparative genomic hybridization identifies virulence differences in streptococcus suis. | streptococcus suis is an important zoonotic pathogen. however, identification of virulent s. suis strains is complicated because of the high diversity of the species. here we evaluated the genetic difference among s. suis strains using comparative genomic hybridization (cgh) and virulence variation in vivo and in vitro. we showed that different clades differed in their ability to activate tlr2/6 in vitro and their capacity to induce cytokine production in vivo as well as their resistance to phag ... | 2014 | 24503649 |
| rare events of intragenus and intraspecies horizontal transfer of the 16s rrna gene. | horizontal gene transfer (hgt) of operational genes has been widely reported in prokaryotic organisms. however, informational genes such as those involved in transcription and translation processes are very difficult to be horizontally transferred, as described by woese's complexity hypothesis. here, we analyzed all of the completed prokaryotic genome sequences (2,143 genomes) in the ncbi (national center for biotechnology information) database, scanned for genomes with high intragenomic heterog ... | 2015 | 26220935 |
| investigation of pathogenesis of h1n1 influenza virus and swine streptococcus suis serotype 2 co-infection in pigs by microarray analysis. | swine influenza virus and streptococcus suis are two important contributors to the porcine respiratory disease complex, and both have significant economic impacts. clinically, influenza virus and streptococcus suis co-infections in pigs are very common, which often contribute to severe pneumonia and can increase the mortality. however, the co-infection pathogenesis in pigs is unclear. in the present study, co-infection experiments were performed using swine h1n1 influenza virus and streptococcus ... | 2015 | 25906258 |
| exposure of feral swine (sus scrofa) in the united states to selected pathogens. | feral swine (sus scrofa) are widely distributed in the united states. in 2011 and 2012, serum samples and tonsils were recovered from 162 and 37 feral swine, respectively, in the us to evaluate exposure to important swine endemic pathogens. antibodies against porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2) were found in 2.5% and 25.3% of tested sera, respectively. positive serological reactions against mycoplasma hyopneumoniae and actinobacillus p ... | 0 | 25673913 |
| evaluation of galleria mellonella larvae for studying the virulence of streptococcus suis. | streptococcus suis is an encapsulated gram-positive bacterium and the leading cause of sepsis and meningitis in young pigs, resulting in considerable economic losses in the porcine industry. s. suis is considered an emerging zoonotic agent with increasing numbers of human cases over the last years. in the environment, both avirulent and virulent strains occur in pigs, with no evidence for consistent adapatation of virulent strains to the human host. currently, there is an urgent need for a conve ... | 2016 | 27978817 |
| identification of a novel host-specific igm protease in streptococcus suis. | streptococcus suis serotype 2 is a highly invasive, extracellular pathogen in pigs with the capacity to cause severe infections in humans. this study was initiated by the finding that igm degradation products are released after opsonization of s. suis. the objective of this work was to identify the bacterial factor responsible for igm degradation. the results of this study showed that a member of the ides family, designated ide(ssuis) (immunoglobulin m-degrading enzyme of s. suis), is responsibl ... | 2012 | 23243300 |
| survey of susceptibility to marbofloxacin in bacteria isolated from diseased pigs in europe. | a monitoring programme of marbofloxacin susceptibility of bacteria from europe causing respiratory tract infection and meningitis in pigs has been active since 1994 and 2002, respectively. monitoring digestive, metritis and urinary tract infection (uti) in pigs has been active since 2005 and susceptibility results until 2013 are presented. minimum inhibitory concentration (mic) was determined by broth microdilution. for mic interpretation, vétoquinol-evaluated breakpoints were applied. for diges ... | 2017 | 28348142 |
| serological patterns of actinobacillus pleuropneumoniae, mycoplasma hyopneumoniae, pasteurella multocida and streptococcus suis in pig herds affected by pleuritis. | respiratory illness is traditionally regarded as the disease of the growing pig, and has historically mainly been associated to bacterial infections with focus on mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae. these bacteria still are of great importance, but continuously increasing herd sizes have complicated the scenario and the influence of secondary invaders may have been increased. the aim of this study was to evaluate the presence of a. pleuropneumoniae and m. hyopneumoniae, ... | 2016 | 27716292 |
| a survey of porcine respiratory disease complex (prdc) associated pathogens among commercial pig farms of korea via oral fluid method. | oral fluid analysis for monitoring herd is an interest in commercial pig farms in korea. the aim of this study was to investigate the positive rates and correlation of eight pathogens associated with porcine respiratory disease complex (prdc) by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. the samples collected by rope-chewing method were analyzed with reverse-transcription polymerase chain reaction (rt-pcr) or standard pcr depending on microorganisms. the pathogens ... | 2016 | 27586468 |
| monitoring of antimicrobial susceptibility of respiratory tract pathogens isolated from diseased cattle and pigs across europe, 2009-2012: vetpath results. | vetpath is an ongoing pan-european antibiotic susceptibility monitoring programme that collects pathogens from diseased cattle, pigs and poultry. in the current study, 996 isolates from cattle and pig respiratory tract infections were tested for their antimicrobial susceptibilities. non-replicate lung samples or nasopharyngeal/nasal swabs were collected from animals with acute clinical signs in 10 countries during 2009-2012. pasteurella multocida, mannheimia haemolytica and histophilus somni fro ... | 2016 | 27102206 |
| preparation, characterization and pharmacokinetics of doxycycline hydrochloride and florfenicol polyvinylpyrroliddone microparticle entrapped with hydroxypropyl-β-cyclodextrin inclusion complexes suspension. | in order to effectively control the bacterial pneumonia in pigs, doxycycline hydrochloride (doxhcl) and florfenicol (ff) microparticle suspension together with inclusion complexes was prepared by using hydroxypropyl-β-cyclodextrin (hp-β-cd) as host molecules, polyvinylpyrroliddone (pvp) as polymer carriers and hydroxypropyl methyl cellulose (hpmc) as suspending agents. in vitro antibacterial activity, properties, stability and pharmacokinetics of the suspension were studied. the results demonstr ... | 2016 | 26918512 |
| canada: distribution of streptococcus suis (from 2012 to 2014) and actinobacillus pleuropneumoniae (from 2011 to 2014) serotypes isolated from diseased pigs. | | 2015 | 26483588 |
| erythema multiforme associated with respiratory disease in a commercial breeding pig herd. | this study describes an erythema multiforme (em) in breeding sows, after their mixing in the group housing system. sows at 30-35 days of gestation showed red and raised skin areas, depression, anorexia, fever, respiratory problems, and increased return to estrus. blood and nasal samples from diseased sows were examined by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay for respiratory pathogens. hematological and biochemical analyses were performed on the blood sampl ... | 2015 | 26266696 |
| antimicrobial susceptibility monitoring of respiratory tract pathogens isolated from diseased cattle and pigs across europe: the vetpath study. | vetpath is an ongoing pan-european antibiotic susceptibility monitoring programme collecting pathogens from diseased antimicrobial non-treated cattle, pigs and poultry. in the current study, 1001 isolates from cattle and pig respiratory tract infections were tested for their antimicrobial susceptibilities. non-replicate lung samples or nasopharyngeal/nasal swabs were collected from animals with acute clinical signs in 11 countries during 2002-2006. pasteurella multocida and mannheimia haemolytic ... | 2014 | 24837878 |
| screening of actinobacillus pleuropneumoniae luxs inhibitors. | luxs, a conserved bacterial enzyme involved in the activated methyl cycle, catalyzes s-ribosylhomocysteine (srh) into homocysteine and ai-2 (the inter-species quorum-sensing signal molecule). this enzyme has been reported to be essential for the survival of actinobacillus pleuropneumoniae in its natural host. therefore, it is a potential drug target against a. pleuropneumoniae, an important swine respiratory pathogen causing great economic losses in the pig industry worldwide. in this study, the ... | 2013 | 23743601 |
| zinc as an agent for the prevention of biofilm formation by pathogenic bacteria. | biofilm formation is important for the persistence of bacteria in hostile environments. bacteria in a biofilm are usually more resistant to antibiotics and disinfectants than planktonic bacteria. our laboratory previously reported that low concentrations of zinc inhibit biofilm formation of actinobacillus pleuropneumoniae. the aim of this study is to evaluate the effect of zinc on growth and biofilm formation of other bacterial swine pathogens. | 2013 | 23509865 |
| haemophilus parasuis serovars isolated from pathological samples in northern italy. | from january 2007 to december 2011, a total of 106 haemophilus parasuis strains isolated from pigs were serotyped by agar gel diffusion test (dg). serovar 4 was the most prevalent (24.5%), followed by serovar 13 (19.8%) and serovar 5 (11.3%). twenty-nine strains were non-typeable (27.3%). the strains were divided into two groups, depending on whether they were isolated from specific pathological lesions of systemic disease such as polyserositis, arthritis or meningitis (73 cases of 106) or from ... | 2013 | 22494561 |
| acute meningitis of piglets and mice caused by co-infected with streptococcus suis and aerococcus viridans. | the two opportunistic pathogens, streptococcus suis (s. suis) and aerococcus. viridans (a. viridans) were isolated from the brains of piglets suffered bacterial meningitis in a farm of china. the murine model has been established to evaluate the pathogenicity and symbiotic relationship of s. suis and a. viridans simultaneously infection. our results demonstrated the ability of new serotype s. suis to cause the classical bacterial meningitis and death were greatly enhanced during co-infection wit ... | 2016 | 27816682 |
| chemical constituents from campylotropis hirtella. | a phytochemical investigation on the roots of campylotropis hirtella afforded nine new isoflavones (3-9, 12, 15), two new isoflavans (10 and 11), one new coumestan (1), and three new prenylated benzoic acid derivatives (2, 13, 14), together with twenty-four known compounds. their structures were established by spectroscopic analysis and circular dichroism data. the isolated compounds were also evaluated for their antibacterial activities against enterococcus faecalis, salmonella gallinarum, stre ... | 2016 | 27220079 |
| further identification of novel lantibiotic operons using lanm-based genome mining. | lantibiotics are gene-encoded antimicrobial peptides that are distinguished by the presence of the unusual structures, lanthionine and β-methyllanthionine, which are introduced through enzyme-catalysed post-translational modification. lantibiotics can be subdivided on the basis of the nature of the enzyme(s) which catalyse this reaction. lantibiotic synthetases, generically designated lanm, which catalyse the dehydration of serines (and threonines) followed by the formation lanthionine (and β-me ... | 2011 | 26781497 |
| investigations of selected pathogens among village pigs in central papua, indonesia. | village pig husbandry is an important part of livestock production in papua province, eastern indonesia. however, high level of disease and mortality constrains production. the aim of this study was to investigate the prevalence of the selected pathogens in village pigs in the jayawijaya region of papua province, indonesia. two studies were conducted: study 1 determined the prevalence of selected pathogens in dead or moribund pigs sent to the main local market for sale. study 2 recorded the prev ... | 2016 | 26381546 |
| identifying associations between pig pathologies using a multi-dimensional machine learning methodology. | abattoir detected pathologies are of crucial importance to both pig production and food safety. usually, more than one pathology coexist in a pig herd although it often remains unknown how these different pathologies interrelate to each other. identification of the associations between different pathologies may facilitate an improved understanding of their underlying biological linkage, and support the veterinarians in encouraging control strategies aimed at reducing the prevalence of not just o ... | 2012 | 22937883 |
| a novel inhibition elisa for the detection and monitoring of penicillium marneffei antigen in human serum. | the thermally dimorphic fungus penicillium marneffei is a causative agent of penicilliosis marneffei, a disease considered to be an acquired immune deficiency syndrome (aids)-defining illness in southeast asia and southern china. we have developed an inhibition enzyme-linked immunosorbent assay (inh-elisa) incorporating the yeast phase specific mannoprotein-binding monoclonal antibody 4d1 for the detection of p. marneffei infection. in our sample set, the test detected antigenemia in all 45 (100 ... | 2016 | 26838686 |
| potential probiotic attributes of a new strain of bacillus coagulans cgmcc 9951 isolated from healthy piglet feces. | a new strain of bacillus coagulans cgmcc 9551, which has a broad range of antibacterial activities against six main pathogenic bacteria including escherichia coli o8, staphylococcus aureus, salmonella enterica subsp. enterica serovar enteritidis, streptococcus suis, listeria monocytogenes and pasteurella multocida, was isolated from healthy piglet feces. in adhesion assay, the isolate exhibited a stronger adhesion to pig intestinal mucus than that of b. subtilis jt143 and l. acidophilus ly24 res ... | 2015 | 25752235 |
| bacterial cell surface properties: role of loosely bound extracellular polymeric substances (lb-eps). | this study investigated the effect of loosely bound extracellular polymeric substances (lb-eps) on the comprehensive surface properties of four bacteria (bacillus subtilis, streptococcus suis, escherichia coli and pseudomonas putida). the removal of lb-eps from bacterial surfaces by high-speed centrifugation (12,000×g) was confirmed by sem images. viability tests showed that the percentages of viable cells ranged from 95.9% to 98.0%, and no significant difference was found after treatment (p>0.0 ... | 2015 | 25805151 |
| high-level expression, purification and characterisation of porcine β-defensin 2 in pichia pastoris and its potential as a cost-efficient growth promoter in porcine feed. | porcine β-defensin 2 (pbd2), a recently discovered porcine defensin that is produced by the intestine, exerts antimicrobial activities and innate immune effects that are linked to intestinal diseases in pigs. here, we report a codon-optimised protein corresponding to mature pbd2 cdna that was expressed and purified in pichia pastoris yeast. the highest amount of secreted protein (3,694.0 mg/l) was reached 144 h into a 150-h induction during high-density cultivation. precipitation followed by gel ... | 2014 | 24515729 |
| combined antibacterial activity of phage lytic proteins holin and lysin from streptococcus suis bacteriophage smp. | development of novel antibacterial agents is required to control infection with multidrug-resistant streptococcus suis. holsmp and lysmp, the holin and lysin of s. suis serotype 2 bacteriophage, named smp, are responsible for lysis of host cells and release of progeny phage. holsmp and lysmp expressed in escherichia coli bl21(de3) exerted efficient activity at 37 °c, ph 5.2, with addition of 0.8 % β-mercaptoethanol. lytic spectra of purified holsmp, lysmp or holsmp + lysmp mixture were investiga ... | 2012 | 22526567 |
| causes and outcomes of sepsis in southeast asia: a multinational multicentre cross-sectional study. | improved understanding of pathogens that cause sepsis would aid management and antimicrobial selection. in this study, we aimed to identify the causative pathogens of sepsis in southeast asia. | 2017 | 28104185 |
| microbiological identification and analysis of swine lungs collected from carcasses in swine farms, china. | the primary objective of this 3 years study was to determine the prevalence of porcine pathogens of the lungs of swine in swine farms in southern china. a total of 5,420 samples were collected from 200 swine farms. the bacterium that was most commonly isolated was streptococcus suis, with 10.24 % of the samples being positive, 114 lungs (2.1 %) were positive for pseudorabies virus and 263 (4.85 %) were positive for classical swine fever virus; much lower than positive for prrsv (15.1 %, p = 0.02 ... | 2013 | 24426158 |
| improved bacterial mutagenesis by high-frequency allele exchange, demonstrated in clostridium difficile and streptococcus suis. | here we show that the frequency of mutant isolation by two-step allele exchange can be improved by increasing the length of homologous dna and the opportunity for recombination, obviating the need for counterselection markers. these principles are demonstrated in clostridium difficile and streptococcus suis but are likely to be generally applicable. | 2013 | 23728809 |
| macedovicin, the second food-grade lantibiotic produced by streptococcus macedonicus aca-dc 198. | streptococcus macedonicus aca-dc 198 was found to produce a second lantibiotic named macedovicin in addition to macedocin. macedovicin was purified to homogeneity and mass spectrometric analysis identified a peptide of approximately 3.4 kda. partial n-terminal sequence analysis and tandem mass spectrometry revealed that macedovicin was identical to bovicin hj50 and thermophilin 1277 produced by streptococcus bovis and streptococcus thermophilus, respectively. macedovicin inhibits a broad spectru ... | 2013 | 23122510 |
| identification and proteome analysis of the two-component virr/virs system in epidemic streptococcus suis serotype 2. | streptococcus suis serotype 2 (ss2) is an important zoonotic pathogen that infects pigs and sporadically causes serious infections in humans. two recent large-scale outbreaks of human streptococcal toxic-shock-like syndrome with high mortality occurred in china, posing new challenges for global public health. however, the global regulation of the virulence of epidemic ss2 isolates lacks a systematic understanding. in this study, we performed a mutational and functional analysis of an ss2 two-com ... | 2012 | 22670712 |
| development and application of loop-mediated isothermal amplification assays based on its-1 for rapid detection of toxoplasma gondii in pork. | the loop-mediated isothermal amplification (lamp) assay is a novel method that rapidly amplifies dna with high specificity and sensitivity under isothermal conditions. in this study, we established a lamp assay with six primers targeting a highly conserved region of toxoplasma gondii its-1 sequence. the amplification protocol completes within 30 min under isothermal condition in a 65°c water bath while specificity tests confirmed no cross-reactivity with dna templates of neospora caninum, eimeri ... | 2015 | 25624074 |
| [construction and identification of recombinant adenovirus of muramidase-released protein gene fragment from streptococcus suis type 2]. | to construct and identify the recombinant adenovirus of muramidase-released protein (mrp) gene fragment from streptococcus suis type 2 (ss2). | 2013 | 23643163 |
| characterisation of a novel integrative and conjugative element icessd9 carrying erm(b) and tet(o) resistance determinants in streptococcus suis, and the distribution of icessd9-like elements in clinical isolates. | this study identified a novel integrative and conjugative element (icessd9) carrying erm(b) and tet(o) resistance determinants in streptococcus suis d9 and determined its prevalence in clinical isolates. comparative genome analysis was performed using mauve and artemis comparison tool visualisation programs. inverse pcr was utilised to detect its circular intermediate. the transfer capacity of icessd9 was evaluated by mating assays using s. suis a7 and enterococcus faecalis jh2-2 as recipients. ... | 2016 | 27531000 |
| colloidal gold-based immunochromatographic strip test compromising optimised combinations of anti-s. suis capsular polysaccharide polyclonal antibodies for detection of streptococcus suis. | a rapid diagnosis kit that detects streptococcus suis (s. suis) antigens from urine with an immunochromatographic stripe (ics) test was developed in this study. the ics test was produced using colloidal gold coated with polyclonal antibodies (pabs) against s. suis. the pabs were developed from rabbits immunised with s. suis serotype 2 capsular polysaccharides (cps). development of the pabs was investigated to establish their binding to cps and to determine the maximum sensitivity of two combinat ... | 2014 | 24800681 |
| [analysis of pathogen spectrum of encephalitis/meningitis in northwestern area of china]. | to learn the characteristics of pathogen spectrum of encephalitis /meningitis in northwestern area of china. | 2013 | 24378133 |
| genomic and proteomic characterization of se-i, a temperate bacteriophage infecting erysipelothrix rhusiopathiae. | a bacteriophage infecting pathogenic erysipelothrix rhusiopathiae was isolated from a swine farm experiencing an outbreak of acute swine erysipelas; we designated this phage se-i. se-i has an icosahedral head, a long tail and a double-stranded dna genome. the 34,997-bp genome has a gc content of 34 % and contains 43 open reading frames (orfs) encoding packaging, structural, lysin-holin, and hypothetical proteins. components of purified se-i were separated using sds-page and analyzed using liquid ... | 2016 | 27541818 |
| roles of the putative type iv-like secretion system key component vird4 and prsa in pathogenesis of streptococcus suis type 2. | streptococcus suis type 2 (ss2) is a zoonotic pathogen causing septic infection, meningitis and pneumonia in pigs and humans. ss2 may cause streptococcal toxic shock syndrome (stss) probably due to excessive release of inflammatory cytokines. a previous study indicated that the vird4 gene in the putative type iv-like secretion system (t4ss) within the 89k pathogenicity island specific for recent epidemic strains contributed to the development of stss. however, the functional basis of vird4 in st ... | 2016 | 27995095 |
| development of a sandwich elisa for ehec o157:h7 intimin γ1. | enterohemorrhagic escherichia coli (ehec) o157:h7 is a zoonotic pathogen of worldwide importance that causes foodborne infections in humans. intimin gamma 1 (intimin γ1) is one of the most important outer membrane proteins required for ehec's intimate adhesion to epithelial cells. here, we generated a polyclonal antibody (pab) and a monoclonal antibody (mab) against intimin γ1 to develop a double antibody sandwich elisa (das-elisa) with increased sensitivity and specificity for measuring ehec o1 ... | 2016 | 27603508 |
| high diversity of viable bacteria isolated from lymph nodes of slaughter pigs and its possible impacts for food safety. | ileocaecal lymph nodes (iclns) of pigs, the key immune inductive site for bacterial systemic invasion, were examined in this study with emphasis on viable and cultivable bacteria. | 2015 | 26283649 |
| cloning, expression, and purification of a new antibacterial substance gene from larvae of musca domestica (diptera: muscidae). | musca domestica l. (diptera: muscidae), the housefly, exhibits unique immune defenses and can produce antibacterial substances upon stimulation with bacteria. on the basis of the cdna library constructed using the suppression subtractive hybridization method, a 1188-bp antibacterial substance gene, which we named as566, was amplified by rapid amplification of cdna ends from m. domestica larva stimulated with salmonella pullorum (enterobacteriaceae: salmonella). in this study, the full-length as5 ... | 2014 | 25434038 |
| [enolase of streptococcus suis serotype 2 is involved in the antiphagocytosis of streptococcus suis]. | to prepare the recombinant enolase of streptococcus suis (sseno), analyze the effect of sseno on the antiphagocytosis by antibody-blocking assay in the human blood bactericidal model, and identify the human fibrinogen (hfg)-binding activity of sseno protein. | 2014 | 25374077 |
| impact of antibiotic use in the swine industry. | antibiotic resistance in bacteria associated with pigs not only affects pig production but also has an impact on human health through the transfer of resistant organisms and associated genes via the food chain. this can compromise treatment of human infections. in the past most attention was paid to glycopeptide and streptogramin resistance in enterococci, fluoroquinolone resistance in campylobacter and multi-drug resistance in escherichia coli and salmonella. while these are still important the ... | 2014 | 24959754 |
| [expression, purification and higg-binding activity identification of truncated streptococcus suis factor h-binding protein]. | to construct a prokaryotic expression vector of the his-tagged truncated factor h-binding protein (fhb) fragments, fhb-n (amino acids 45-344aa) and fhb-c (amino acids 345-644aa), of streptococcus suis serotype 2, express it in e.coli bl21 (de3) in order to acquire high-purity recombinant protein, and finally identify the binding activity with human serum igg (higg). | 2014 | 24796751 |
| antimicrobial susceptibility of escherichia coli f4, pasteurella multocida, and streptococcus suis isolates from a diagnostic veterinary laboratory and recommendations for a surveillance system. | antimicrobial susceptibility data on escherichia coli f4, pasteurella multocida, and streptococcus suis isolates from ontario swine (january 1998 to october 2010) were acquired from a comprehensive diagnostic veterinary laboratory in ontario, canada. in relation to the possible development of a surveillance system for antimicrobial resistance, data were assessed for ease of management, completeness, consistency, and applicability for temporal and spatial statistical analyses. limited farm locati ... | 2014 | 24688133 |
| a functional peptidoglycan hydrolase characterized from t4ss in 89k pathogenicity island of epidemic streptococcus suis serotype 2. | streptococcus suis serotype 2 (s. suis 2) has evolved efficient mechanisms to cause streptococcal toxic shock syndrome (stss), which is a new emerging infectious disease linked to s. suis. we have previously reported that a type iv secretion system (t4ss) harbored by the specific 89k pathogenicity island (pai) of s. suis 2 contributes to the development of stss and mediates horizontal transfer of 89k. however, the 89k t4ss machinery assembly in vivo and in vitro is poorly understood, and the com ... | 2014 | 24655418 |
| adhesion of bacterial pathogens to soil colloidal particles: influences of cell type, natural organic matter, and solution chemistry. | bacterial adhesion to granular soil particles is well studied; however, pathogen interactions with naturally occurring colloidal particles (<2 μm) in soil has not been investigated. this study was developed to identify the interaction mechanisms between model bacterial pathogens and soil colloids as a function of cell type, natural organic matter (nom), and solution chemistry. specifically, batch adhesion experiments were conducted using nom-present, nom-stripped soil colloids, streptococcus sui ... | 2014 | 24495985 |
| multiple-class antimicrobial resistance surveillance in swine escherichia coli f4, pasteurella multocida and streptococcus suis isolates from ontario and the impact of the 2004-2006 porcine circovirus type-2 associated disease outbreak. | the objective of this work was to describe trends in multiple-class antimicrobial resistance present in clinical isolates of escherichia coli f4, pasteurella multocida and streptococcus suis from ontario swine 1998-2010. temporal changes in multiple-class resistance varied by the pathogens examined; significant yearly changes were apparent for the e. coli and p. multocida data. although not present in the e. coli data, significant increases in multiple-class resistance within p. multocida isolat ... | 2014 | 24309129 |